CN113549552B - Umbilical cord placenta collection liquid preparation device - Google Patents
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- CN113549552B CN113549552B CN202110919597.XA CN202110919597A CN113549552B CN 113549552 B CN113549552 B CN 113549552B CN 202110919597 A CN202110919597 A CN 202110919597A CN 113549552 B CN113549552 B CN 113549552B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
本发明提供一种脐带胎盘采集液配液装置，涉及干细胞培育领域。该脐带胎盘采集液配液装置。该脐带胎盘采集液配液装置包括固定台、控制面板、电动滑台、第一支撑杆、环形培养皿和注液机构，控制面板设置在固定台的一侧外壁，固定台的顶端外壁设置有电动滑轨，且第一支撑杆的底端外壁与电动滑轨滑动连接，第一支撑杆靠近顶端的外壁固定连接有固定头，环形培养皿上开设有可闭合的通液口，环形培养皿的一侧外壁开设有固定槽，且固定槽与固定头相适配，注液机构设置在固定台的另一侧的顶端外壁。该脐带胎盘采集液配液装置能够对环形培养皿的内部输入培养液，能够定时定量对环形培养皿中自动添加培养液，提高了培育效果。
The present invention provides a liquid preparation device for collecting umbilical cord and placenta, and relates to the field of stem cell cultivation. The liquid preparation device for collecting umbilical cord and placenta. The liquid preparation device for collecting umbilical cord and placenta comprises a fixed platform, a control panel, an electric slide, a first support rod, an annular culture dish and a liquid injection mechanism, wherein the control panel is arranged on an outer wall of one side of the fixed platform, an electric slide is arranged on the outer wall of the top end of the fixed platform, and the outer wall of the bottom end of the first support rod is slidably connected to the electric slide, a fixed head is fixedly connected to the outer wall of the first support rod near the top end, a closable liquid passage is provided on the annular culture dish, a fixed groove is provided on the outer wall of one side of the annular culture dish, and the fixed groove is adapted to the fixed head, and the liquid injection mechanism is arranged on the outer wall of the top end of the other side of the fixed platform. The liquid preparation device for collecting umbilical cord and placenta can input culture fluid into the inside of the annular culture dish, and can automatically add culture fluid to the annular culture dish at a fixed time and quantity, thereby improving the cultivation effect.
Description
Technical Field
The invention relates to the technical field of stem cell culture, in particular to a liquid preparation device for umbilical cord placenta collection liquid.
Background
The umbilical cord mesenchymal stem cells refer to multifunctional stem cells existing in umbilical cord tissues of newborns, can differentiate into various tissue cells, and have wide clinical application prospects. The human umbilical cord mesenchymal stem cells can be successfully amplified by using the inactivated umbilical cord serum culture system, and the cultured cells have the basic characteristics of the mesenchymal stem cells, so that theoretical basis is provided for establishing a mesenchymal stem cell library and clinical application.
In the storage process of stem cell collection liquid and preparation liquid, partial stem cells are easy to deposit at the bottom, so that nutrient substances obtained by cells at the bottom are poor, the cultivation effect of the cells is affected, and meanwhile, the opening is opened at regular time in the storage device to supplement cultivation liquid, so that the burden of staff is increased.
Disclosure of Invention
(One) solving the technical problems
Aiming at the defects of the prior art, the invention provides a liquid preparation device for umbilical cord placenta collection liquid, which solves the problems that partial stem cells are easy to deposit at the bottom, so that the nutrition substances acquired by the cells at the bottom are poor and the cultivation effect of the cells is affected.
(II) technical scheme
In order to achieve the above purpose, the invention is realized by the following technical scheme: the utility model provides a liquid device is joined in marriage to umbilical cord placenta collection liquid, includes fixed station, control panel, electronic slip table, first bracing piece, annular culture dish and notes liquid mechanism, control panel sets up one side outer wall of fixed station, the top outer wall of fixed station is provided with electronic slide rail, just the bottom outer wall of first bracing piece with electronic slide rail sliding connection, the outer wall fixedly connected with fixed head that first bracing piece is close to the top, the closable logical liquid mouth has been seted up on the annular culture dish, the fixed slot has been seted up to one side outer wall of annular culture dish, just the fixed slot with fixed head looks adaptation, annotate liquid mechanism setting the top outer wall of the opposite side of fixed station.
Preferably, the liquid injection mechanism comprises a liquid storage box and a second support rod, a hydraulic pump is arranged on the inner wall of the bottom end of the liquid storage box, the second support rod is symmetrically arranged with the first support rod, a liquid through pipe is arranged in the second support rod, and one end of the liquid through pipe is communicated with the output end of the hydraulic pump.
Preferably, the inner wall sliding connection of second bracing piece has electric telescopic handle, electric telescopic handle is close to the outer wall of one end and has seted up the play liquid groove that distributes with electric telescopic handle axis equidistance, electric telescopic handle's the other end outer wall with the other end of leading to the liquid pipe is linked together.
Preferably, the annular culture dish is kept away from the one end outer wall of fixed slot has seted up the liquid mouth, the liquid mouth with electric telescopic handle looks adaptation.
Preferably, the liquid through hole is fixedly connected with a fixing plate, and a first communication groove corresponding to the liquid outlet groove is formed in the fixing plate.
Preferably, the inner wall fixedly connected with rotation motor of annular culture dish, just rotation motor's one end fixedly connected with rotor plate, the second intercommunication groove that the equidistance was distributed has been seted up on the rotor plate.
Preferably, a charging plate is arranged on the inner wall of the top end of the fixed table, an induction plate is arranged on the outer wall of the annular culture dish, and the induction plate is electrically connected with the rotating motor.
(III) beneficial effects
(1) The umbilical cord placenta collection liquid preparation device can input culture liquid into the annular culture dish, can automatically add the culture liquid into the annular culture dish at regular time and quantity, and improves the cultivation effect.
(2) According to the umbilical cord placenta collection liquid preparation device, the annular culture dish can rotate, so that the effect of cultivation is prevented from being influenced by cell deposition at the bottom, and after the culture liquid is injected, the culture liquid can be rapidly and uniformly distributed everywhere, so that the concentration of the upper part and the lower part of the culture liquid in the annular culture dish is kept balanced.
Drawings
FIG. 1 is a perspective view of a liquid preparation device for umbilical cord placenta collection liquid;
FIG. 2 is a perspective view showing the structure of the umbilical cord placenta collection fluid preparation device with the annular culture dish removed;
FIG. 3 is a cross-sectional view of the structure at the stationary stage of the present invention;
FIG. 4 is a cross-sectional view of the annular culture dish according to the invention;
Fig. 5 is an enlarged view of the structure at a of the present invention.
The device comprises a fixed table-1, a control panel-2, an electric sliding table-3, a first supporting rod-4, an annular culture dish-5, a fixed head-6, a second supporting rod-7, an electric telescopic rod-8, a liquid storage box-9, a hydraulic pump-10, a liquid passing pipe-11, a liquid outlet groove-12, a liquid passing port-13, a fixed groove-14, a fixed plate-15, a rotating motor-16, a rotating plate-17, a charging plate-18 and an induction plate-19.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples
As shown in fig. 1-5, an embodiment of the invention provides a liquid preparation device for umbilical cord placenta collection liquid, which comprises a fixed table 1, a control panel 2, an electric sliding table 3, a first support rod 4, an annular culture dish 5 and a liquid injection mechanism, wherein the control panel 2 is arranged on one side outer wall of the fixed table 1, an electric sliding rail is arranged on the top end outer wall of the fixed table 1, the bottom end outer wall of the first support rod 4 is in sliding connection with the electric sliding rail, a fixed head 6 is fixedly connected with the outer wall of the first support rod 4 close to the top end, a closable liquid through hole is formed in the annular culture dish 5, a fixed groove 14 is formed in one side outer wall of the annular culture dish 5, the fixed groove 14 is matched with the fixed head 6, and the liquid injection mechanism is arranged on the top end outer wall of the other side of the fixed table 1.
In the invention, the liquid injection mechanism comprises a liquid storage box 9 and a second support rod 7, wherein a hydraulic pump 10 is arranged on the inner wall of the bottom end of the liquid storage box 9, the second support rod 7 and the first support rod 4 are symmetrically arranged, a liquid through pipe 11 is arranged in the second support rod 7, and one end of the liquid through pipe 11 is communicated with the output end of the hydraulic pump 10.
In the invention, the inner wall of the second supporting rod 7 is connected with an electric telescopic rod 8 in a sliding way, the outer wall of one end, close to the electric telescopic rod 8, is provided with liquid outlet grooves 12 which are distributed at equal intervals on the central axis of the electric telescopic rod 8, and the outer wall of the other end of the electric telescopic rod 8 is communicated with the other end of a liquid through pipe 11.
In the invention, the outer wall of one end of the annular culture dish 5 far away from the fixed groove 14 is provided with the liquid through hole 13, and the liquid through hole 13 is matched with the electric telescopic rod 8.
In the invention, a fixed plate 15 is fixedly connected to a liquid inlet 13, and a first communication groove corresponding to a liquid outlet groove 12 is formed on the fixed plate 15.
In the invention, the inner wall of the annular culture dish 5 is fixedly connected with a rotating motor 16, one end of the rotating motor 16 is fixedly connected with a rotating plate 17, and the rotating plate 17 is provided with second communicating grooves which are distributed at equal intervals.
In the invention, a charging plate 18 is arranged on the inner wall of the top end of the fixed table 1, an induction plate 19 is arranged on the outer wall of the annular culture dish 5, and the induction plate 19 is electrically connected with a rotating motor 16.
Working principle: during the use, control electronic slip table 3 moves to the outside, this moment, the distance between first bracing piece 4 and second bracing piece 7 increases, can be fixed mutually fixed with fixed head 6 with fixed slot 14 on the annular culture dish 5, it is fixed to reset electronic slip table 3, can fix through opening 13 departments through starting electronic telescopic handle 8, through the electric motor in the first bracing piece 4, can rotate annular culture dish 5, thereby make the cell culture liquid in the annular culture dish 5 rotate thereupon, prevent that the cell deposit from influencing cultivation or storing the effect in the bottom of annular culture dish 5, because the play cistern 12 has been seted up to the outer wall of electronic telescopic handle 8, through starting hydraulic pump 10, can carry the culture liquid in the receiver 9 to leading to liquid pipe 11, start through starting rotating motor 16, rotating motor 16 can drive the rotation board 17 and rotate, the second intercommunication groove on the rotation board 17 communicates with each other with the first intercommunication groove on the fixed plate 15, can be to the inside input liquid to annular culture dish 5 through electronic telescopic handle 8 at this moment, can regularly add the culture liquid to the annular culture dish 5, improve the cell culture liquid in the automatic feed-through motor, prevent cell culture liquid deposit in the annular culture dish 5 from influencing cultivation or storing effect, the bottom of cell culture dish 5 has been realized, the effect can not be evenly distributed in the time, can be reached at the bottom of the fixed plate that the rotation of the fixed plate is realized in the rotation of the annular culture plate 17 is realized after the time, the concentration is realized, the cell culture liquid is filled up in the bottom is completely, and the medium is completely evenly filled with the culture liquid, and can be evenly rotates in the culture liquid, and can be filled up in the culture liquid, and can be well is in the concentration, and can be well up in the concentration, the culture plate 5.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims.
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| CN202110919597.XA CN113549552B (en) | 2021-08-11 | 2021-08-11 | Umbilical cord placenta collection liquid preparation device |
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| CN202110919597.XA CN113549552B (en) | 2021-08-11 | 2021-08-11 | Umbilical cord placenta collection liquid preparation device |
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| CN113549552B true CN113549552B (en) | 2024-05-31 |
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| CN118995420B (en) * | 2024-10-25 | 2024-12-27 | 深圳知因细胞生物科技有限公司 | Bionic culture device for embryonic stem cells |
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| CN112471349A (en) * | 2020-12-11 | 2021-03-12 | 南京绿瞬电子科技有限公司 | Pig feed and preparation method thereof |
| CN112760223A (en) * | 2021-01-22 | 2021-05-07 | 宋铁英 | Stem cell culture system and culture method thereof |
| CN213295382U (en) * | 2020-08-31 | 2021-05-28 | 山西医科大学 | Three-dimensional breeding device of tumour cell for anti-tumor experiments |
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2021
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| CN2398289Y (en) * | 1999-11-16 | 2000-09-27 | 中国科学院力学研究所 | Hollow rotary disc-type three-D cell culturing apparatus with on-line air-supply and liquid supply |
| KR200283693Y1 (en) * | 2002-04-29 | 2002-07-27 | 주식회사 셀론텍 | Apparatus for collecting a blood from Placenta and umbilical cord |
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| JP6759497B1 (en) * | 2019-11-27 | 2020-09-23 | 浙江大学Ｚｈｅｊｉａｎｇ Ｕｎｉｖｅｒｓｉｔｙ | Method for culturing fertilized sheep eggs with high survival rate for in vitro culture of embryos |
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