CN213295382U - Three-dimensional breeding device of tumour cell for anti-tumor experiments - Google Patents
Three-dimensional breeding device of tumour cell for anti-tumor experiments Download PDFInfo
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- CN213295382U CN213295382U CN202021864808.1U CN202021864808U CN213295382U CN 213295382 U CN213295382 U CN 213295382U CN 202021864808 U CN202021864808 U CN 202021864808U CN 213295382 U CN213295382 U CN 213295382U
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- 239000002699 waste material Substances 0.000 claims abstract description 15
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- 230000012010 growth Effects 0.000 claims description 7
- 239000011148 porous material Substances 0.000 claims description 4
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Abstract
本实用新型公开了一种抗肿瘤实验用肿瘤细胞三维培育装置，包括外壳体；所述外壳体的外侧面前端设有控制开关组和触控显示器，外壳体的内侧面中部设有培育舱，培育舱的上端面为敞开设置，培育舱的内侧面底部设有第二电动伸缩杆，本抗肿瘤实验用肿瘤细胞三维培育装置，可以在降低培育中的癌细胞感染的情况下，实时观测癌细胞的生长，同时又可以很方便的进行培养液更换，气动伸缩杆的设置方便了密封罩的开启与关闭，触控显示器的设置方便了癌细胞的观测，通过气泵可以对外壳体内部的空气进行快速交换，通过储液箱可以对培养液进行储存，通过纳米级过滤网可以对培育中的癌细胞支撑，废液箱的设置方便了废液的收集。
The utility model discloses a three-dimensional culturing device for tumor cells for anti-tumor experiments. The upper end face of the incubation cabin is open, and the bottom of the inner side of the incubation cabin is provided with a second electric telescopic rod. This three-dimensional tumor cell incubation device for anti-tumor experiments can observe cancer cells in real time while reducing the infection of the cells being cultivated. The growth of cells, and at the same time, it is very convenient to replace the culture medium. The setting of the pneumatic telescopic rod facilitates the opening and closing of the sealing cover. The setting of the touch display facilitates the observation of cancer cells. For rapid exchange, the culture solution can be stored through the liquid storage tank, and the cancer cells in cultivation can be supported through the nano-scale filter. The setting of the waste liquid tank facilitates the collection of waste liquid.
Description
Technical Field
The utility model relates to a cell cultivation technical field specifically is a three-dimensional breeding device of tumour cell for anti-tumor experiments.
Background
Tumor cell culture is the basis and guarantee for a series of oncology experiments, and only cells with good growth states can show real and reliable results in the subsequent experiment process. Cell culture is considered the most common experiment, but it is not easy to culture cells in good condition. The factors influencing the cell growth are many, and the problem in any one link can cause the progress of the experimental process to be difficult, thereby causing the waste of time and economic cost.
With the development of technology and improvements in culture methods, it has been discovered in recent years that gene expression, signal transduction, and morphology of cells cultured in vitro in two dimensions may differ from those of cells in an organism. At present, two-dimensional cell culture is used for in vitro cell research, which can only partially reflect cytological characteristics, and normal forms of cells are changed after in vitro culture for several generations, and biochemical characteristics and normal biological functions of the cells are changed, so that the effectiveness of experimental results is reduced. With the establishment of the three-dimensional cell culture method, an efficient research means is provided for the work, the three-dimensional cell culture completely simulates the internal environment of an organism, the cultured cells have the optimal viability and differentiation degree, and the evaluation of a drug test is more effective and accurate. Scientists have studied the formation rate, size and survival rate of human glioblastoma cells and the function of protein expression using two-dimensional and three-dimensional cell culture methods, and have found that three-dimensional cultured cells express appropriate proteins, while the corresponding two-dimensional cultured cells do not. It can be seen that different culture methods have direct effects on cell function and morphology.
The development of three-dimensional cell culture bioreactors is rapid, and the three-dimensional cell culture bioreactors are various in variety and different in characteristics, and are provided for the optimum growth environment of cells in different ways, and currently, various bioreactors of a stirring type, a hollow fiber type, a rotating type and the like are typical. However, when the existing three-dimensional cell culture device is used for culturing cancer cells, the culture device needs to be opened frequently to observe the growth condition of the cells, so that the cancer cells during culturing are easily polluted, meanwhile, the culture solution needs to be replaced frequently in the growth process of some cancer cells, and the existing three-dimensional cell culture device is complicated when the culture solution is replaced, has more operation steps and is inconvenient to use.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to overcome current defect, provide a three-dimensional breeding device of tumour cell for anti-tumor experiments, can be convenient carry out the culture solution and change, can survey the cancer cell in cultivating in real time again simultaneously, avoided the infection of cancer cell, can effectively solve the problem in the background art.
In order to achieve the above object, the utility model provides a following technical scheme: a three-dimensional tumor cell culturing device for anti-tumor experiments comprises an outer shell; the front end of the outer side surface of the outer shell is provided with a control switch group and a touch display, the middle part of the inner side surface of the outer shell is provided with a cultivation cabin, the upper end surface of the cultivation cabin is open, the bottom of the inner side surface of the cultivation cabin is provided with a second electric telescopic rod, the upper end surface of the second electric telescopic rod is provided with a cultivation box, the middle part of the inner side surface of the cultivation box is provided with a nano-scale filter screen, the upper surface of the nano-scale filter screen is provided with a cell support, the right end of the inner side surface of the outer shell is provided with a liquid pump, a first electric telescopic rod, a liquid storage box and an air pump, the air inlet of the air pump is communicated with the outside, the air outlet of the air pump is connected with the cultivation cabin through a soft conduit, the first electric telescopic rod is connected with a, the reference column rotates through the hinge that sets up at its left end face and is connected with the shower nozzle, and the outside front end of shower nozzle is equipped with the camera, and the input of control switch group is connected with external power source's output electricity, and the output of control switch group is connected with the liquid pump respectively, first electric telescopic handle, air pump, second electric telescopic handle, positioning motor and fourth electric telescopic handle's input electricity, and control switch group is connected with touch-control display and the two-way electricity of camera respectively.
Furthermore, four support legs are uniformly distributed on the periphery of the lower surface of the outer shell, the outer shell is rotatably connected with a sealing cover plate through pneumatic telescopic rods arranged at the left end and the right end of the upper surface of the outer shell, and the input end of each pneumatic telescopic rod is electrically connected with the output end of the control switch group.
Furthermore, a pore structure is arranged in the cell scaffold, and the size of pores meets the requirements of growth and transplantation of tumor cells on the scaffold.
Furthermore, the spray head is rotatably connected with a third electric telescopic rod through a hinge arranged on the outer side face of the spray head, the end part of the third electric telescopic rod is rotatably connected with the outer side face of the rotary table through a hinge, and the input end of the third electric telescopic rod is electrically connected with the output end of the control switch group.
Furthermore, the liquid inlet of the spray head is connected with the liquid outlet of the liquid pump through a soft conduit, and the liquid inlet of the liquid pump is connected with the liquid storage tank through a conduit.
Furthermore, the lateral surface left end of shell body is equipped with the waste liquid case, and the waste liquid case passes through the back flow and links to each other with the lateral surface lower extreme of artificial containers, is equipped with first solenoid valve on the back flow, and the input of second solenoid valve is connected with the output electricity of control switch group.
Further, the outer side upper end of the cultivation cabin is connected with the outer side of the outer shell through the exhaust pipe, the exhaust pipe is provided with a second electromagnetic valve, and the input end of the second electromagnetic valve is electrically connected with the output end of the control switch group.
Furthermore, both ends all are equipped with the closing plate about the lateral surface of shell body.
Compared with the prior art, the beneficial effects of the utility model are that: this three-dimensional breeding device of tumour cell for anti-tumor experiments has following benefit:
1. this three-dimensional breeding device of tumour cell for anti-tumor experiments can reduce under the condition of the cancer cell infection in cultivating, observes cancer cell's growth in real time, and the culture solution that carries on that simultaneously can be very convenient again is changed, and pneumatic telescopic link's setting has made things convenient for opening and closing of sealed cowling.
2. The touch display set up the observation that has made things convenient for the cancer cell, can carry out quick exchange to the inside air of shell body through the air pump, can store the culture solution through the liquid reserve tank, can support the cancer cell in cultivating through the nanometer filter screen, the waste liquid case set up the collection that has made things convenient for the waste liquid.
Drawings
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is a sectional view of the present invention;
FIG. 3 is an enlarged view of a portion A;
fig. 4 is a left side view of the present invention.
In the figure: 1 pneumatic telescopic link, 2 control switch group, 3 cultivate the cabin, 4 touch display, 5 stabilizer blades, 6 outer casings, 7 liquid pumps, 8 first electric telescopic handle, 9 liquid storage tanks, 10 air pumps, 11 culture dishes, 12 sealing plates, 13 back flow pipes, 14 nanometer filter screens, 15 second electric telescopic handle, 16 artificial containers, 17 cell support, 18 waste liquid boxes, 19 blast pipes, 20 support frames, 21 carousel, 22 shower nozzles, 23 cameras, 24 reference columns, 25 third electric telescopic handle, 26 motor that transposes, 27 fourth electric telescopic handle.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a three-dimensional tumor cell culturing device for anti-tumor experiments comprises an outer shell 6; four support legs 5 are uniformly distributed around the lower surface of the outer shell 6, the outer shell 6 is rotatably connected with a sealing cover plate through pneumatic telescopic rods 1 arranged at the left end and the right end of the upper surface of the outer shell, the input end of the pneumatic telescopic rod 1 is electrically connected with the output end of a control switch group 2, the pneumatic telescopic rod 1 is convenient to open and close in a sealing way, the front end of the outer side surface of the outer shell 6 is provided with the control switch group 2 and a touch display 4, the touch display 4 is convenient to observe cancer cells, a cultivation cabin 3 is arranged in the middle of the inner side surface of the outer shell 6, the upper end surface of the cultivation cabin 3 is open, a second electric telescopic rod 15 is arranged at the bottom of the inner side surface of the cultivation cabin 3, a cultivation box 16 is arranged on the upper end surface of the second electric telescopic rod 15, a nano-scale filter screen 14 is, the upper surface of the nanometer filter screen 14 is provided with a cell support 17, the inside of the cell support 17 is provided with a hole structure, the size of the hole meets the requirements of growth and transplantation of tumor cells on the support, the right end of the inner side surface of the outer shell 6 is provided with a liquid pump 7, a first electric telescopic rod 8, a liquid storage tank 9 and an air pump 10, the air inlet of the air pump 10 is communicated with the outside, the air outlet of the air pump 10 is connected with the cultivation cabin 3 through a soft conduit, the air inside the outer shell 6 can be rapidly exchanged through the air pump 10, the first electric telescopic rod 8 is connected with a fourth electric telescopic rod 27 through a support frame 20 arranged on the upper end surface of the first electric telescopic rod, the output shaft of the fourth electric telescopic rod 27 is connected with a positioning motor 26, the output shaft of the positioning motor 26 is connected with a turntable 21, the middle part of the, the spray head 22 is rotatably connected with a third electric telescopic rod 25 through a hinge arranged on the outer side surface thereof, the end part of the third electric telescopic rod 25 is rotatably connected with the outer side surface of the rotary disc 21 through a hinge, the input end of the third electric telescopic rod 25 is electrically connected with the output end of the control switch group 2, the liquid inlet of the spray head 22 is connected with the liquid outlet of the liquid pump 7 through a soft conduit, the liquid inlet of the liquid pump 7 is connected with the liquid storage tank 9 through a conduit, the front end of the outer side surface of the spray head 22 is provided with a camera 23, the input end of the control switch group 2 is electrically connected with the output end of an external power supply, the left end of the outer side surface of the outer shell 6 is provided with a waste liquid tank 18, the waste liquid tank 18 is connected with the lower end of the outer side surface of the incubator 16 through a return pipe 13, the waste liquid, cultivate the lateral surface upper end of cabin 3 and link to each other with shell body 6's lateral surface through blast pipe 19, be equipped with the second solenoid valve on the blast pipe 19, the input of second solenoid valve is connected with control switch group 2's output electricity, both ends all are equipped with closing plate 12 about shell body 6's lateral surface, control switch group 2's output respectively with liquid pump 7, first electric telescopic handle 8, air pump 10, second electric telescopic handle 15, positioning motor 26 and fourth electric telescopic handle 27's input electricity are connected, control switch group 2 is connected with touch display 4 and camera 23 both-way electricity respectively, this three-dimensional breeding device of tumour cell for anti-tumour experiment, can be under the condition that reduces the cancer cell infection in cultivating, observe the growth of cancer cell in real time, simultaneously again can be very convenient carry out the culture solution and change.
When in use: switch on external power supply, will the utility model discloses arrange in the thermostated container after the disinfection, it is inside to transplant cancer cell to cell support 17, with the inside of cell culture liquid injection liquid reserve tank 9, by liquid pump 7 with nutrient solution pump-feed to shower nozzle 22, the culture solution sprays on cell support 17 through shower nozzle 22, make the inside of culture solution is soaked to the bottom of cell support 17, when needs carry out the culture solution and change, open first solenoid valve, 11 inside waste liquid of culture dish enters into the inside of waste liquid case 18 via back flow 13, when needs observe cancer cell's growth process, need not to open sealed apron, can carry out simple observation to the cell growth condition through camera 23.
It should be noted that the core chip of the control switch group 2 disclosed in this embodiment is a PLC single chip, the specific model is siemens S7-200, and the pneumatic telescopic rod 1, the liquid pump 7, the first electric telescopic rod 8, the air pump 10, the second electric telescopic rod 15, the third electric telescopic rod 25, the positioning motor 26, and the fourth electric telescopic rod 27 may be freely configured according to the actual application scenario. The control switch group 2 controls the pneumatic telescopic rod 1, the liquid pump 7, the first electric telescopic rod 8, the air pump 10, the second electric telescopic rod 15, the third electric telescopic rod 25, the positioning motor 26 and the fourth electric telescopic rod 27 to work by adopting a common method in the prior art.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
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| CN202021864808.1U CN213295382U (en) | 2020-08-31 | 2020-08-31 | Three-dimensional breeding device of tumour cell for anti-tumor experiments |
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| CN202021864808.1U CN213295382U (en) | 2020-08-31 | 2020-08-31 | Three-dimensional breeding device of tumour cell for anti-tumor experiments |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113549552A (en) * | 2021-08-11 | 2021-10-26 | 安徽惠恩生物科技股份有限公司 | Liquid collecting and distributing device for umbilical cord placenta |
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2020
- 2020-08-31 CN CN202021864808.1U patent/CN213295382U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113549552A (en) * | 2021-08-11 | 2021-10-26 | 安徽惠恩生物科技股份有限公司 | Liquid collecting and distributing device for umbilical cord placenta |
| CN113549552B (en) * | 2021-08-11 | 2024-05-31 | 安徽惠恩生物科技股份有限公司 | Umbilical cord placenta collection liquid preparation device |
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