CN115287188B - Stem cell culture instrument - Google Patents
Stem cell culture instrument Download PDFInfo
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- CN115287188B CN115287188B CN202210959041.8A CN202210959041A CN115287188B CN 115287188 B CN115287188 B CN 115287188B CN 202210959041 A CN202210959041 A CN 202210959041A CN 115287188 B CN115287188 B CN 115287188B
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 32
- 238000004113 cell culture Methods 0.000 title claims abstract description 13
- 238000007789 sealing Methods 0.000 claims abstract description 61
- 230000008878 coupling Effects 0.000 claims abstract description 15
- 238000010168 coupling process Methods 0.000 claims abstract description 15
- 238000005859 coupling reaction Methods 0.000 claims abstract description 15
- 238000009423 ventilation Methods 0.000 claims description 12
- 230000005540 biological transmission Effects 0.000 claims description 7
- 230000001360 synchronised effect Effects 0.000 claims description 7
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000002146 bilateral effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 238000010370 cell cloning Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a culture instrument, in particular to a stem cell culture instrument. It is necessary to design a stem cell culture apparatus which is convenient to fix culture vessels and does not increase the working strength of staff. The utility model provides a stem cell's culture apparatus, including sealed underframe, support chassis, observation window, sealed cabinet door and control panel, sealed underframe lower part rigid coupling has the support chassis, and sealed underframe upper portion embedding is equipped with the observation window, and sealed underframe front portion left side rotation is equipped with sealed cabinet door, is equipped with the control panel in the middle of the sealed cabinet door left portion. According to the invention, the culture dish filled with stem cells is placed on the supporting tray, the sealing cabinet door is pulled to swing backwards for resetting, the positioning sliding block moves rightwards for resetting to enable the positioning rope to be loosened, the right positioning screw rod is driven by the resetting belt component to rotate forwards for resetting due to the action of the resetting spring, and the soft rope limiting frame also moves downwards for resetting to limit and fix the culture dish, so that people can conveniently fix the culture dish.
Description
Technical Field
The invention relates to a culture instrument, in particular to a stem cell culture instrument.
Background
Cell culture, also called cell cloning technology, is a normal term in biology as cell culture technology, which is an essential process for either whole bioengineering technology or for one of the biological cloning technologies.
Patent application CN111518692A discloses an automatic culture instrument of human stem cells, including cultivateing the appearance body, cultivate this internal culture room that is equipped with of appearance, be equipped with the base in the culture room, the conical frame, culture flask and motor, conical frame and base rotate and connect, motor drive conical frame rotates, the culture flask stacks up and fixes on the conical frame, be located the distance of culture flask at last layer to conical frame center and be less than the distance of culture flask at next layer to conical frame center, the same layer's culture flask is the same to conical frame center's distance, one side that the conical frame center was kept away from to the culture flask is equipped with first through-hole, first through-hole penetrates first inverted U-shaped pipe, the bottom in the culture flask is arranged in to first inverted U-shaped pipe one end, the other end is equipped with the bifurcation pipe, bifurcation respectively communicates two at least culture flasks of next adjacent layer. The invention provides an automatic human stem cell culture instrument which can automatically divide stem cells in an original culture bottle into other culture bottles in a halving way, and is convenient to operate. Although the above patent can realize the cultivation of stem cells, the fixing mode of the culture vessel is complex, the operation is complex, and the working strength of staff is increased.
Based on the defects in the above patent, we propose a stem cell culture apparatus which is convenient to fix culture vessels and does not increase the working strength of staff.
Disclosure of Invention
In order to overcome the defects that the fixing mode of the culture vessel is complex, the operation is complicated and the working strength of staff is increased in the patent, the invention provides the stem cell culture instrument which is convenient for fixing the culture vessel and does not increase the working strength of the staff.
The technical scheme of the invention is as follows:
The utility model provides a stem cell's culture apparatus, including sealed base box, support chassis, the observation window, sealed cabinet door, control screen and hold the tray of putting, sealed base box lower part rigid coupling has the support chassis, sealed base box upper portion embedded be equipped with the observation window, sealed base box front portion left side rotation is equipped with sealed cabinet door, be equipped with the control screen in the middle of the sealed cabinet door left part, sealed base box medial surface lower part rigid coupling has holds the tray of putting, still including ventilation mechanism and locking mechanical system, be provided with the ventilation mechanism that is used for the exhaust air on the sealed base box, be provided with the locking mechanical system who is used for locking the door plant between sealed cabinet door and the sealed base box.
Further, ventilation mechanism is including air duct, constant temperature end cover, servo motor, drive shaft, fan and intake pipe, and sealed end case upper portion is equipped with the air duct, and sealed end case lateral surface lower part rigid coupling has the constant temperature end cover, and constant temperature end cover middle part rigid coupling has servo motor, control screen and servo motor electric connection, is connected with the drive shaft on servo motor's the output shaft, and the constant temperature end cover both sides all rotate and are equipped with the fan, pass through the hold-in range transmission between fan and the drive shaft both sides, are connected with four intake pipes between air duct and the constant temperature end cover.
Further, locking mechanical system is including lock plate, location slider, location magnetic pole, articulated connecting rod and slip locking piece, and sealing cabinet door right part upper and lower symmetry rotation is equipped with the lock plate, and sealing bottom box right part front side upper and lower symmetry slip is equipped with the slip locking piece, and upper and lower both sides slip locking piece contacts with upper and lower both sides lock plate respectively, and sealing bottom box front portion upside rigid coupling has the location magnetic pole, and the slip is equipped with the location slider on the location magnetic pole, and the rotation is equipped with articulated connecting rod between location slider and the sealing cabinet door.
Further, the limiting mechanism comprises a positioning rope, a positioning screw rod, a rope wheel, a soft rope limiting frame, a reset belt assembly and a reset spring, wherein the middle parts of the left side and the right side of the sealed bottom box are rotatably provided with the positioning screw rod, the left side and the right side of the positioning screw rod are in transmission through a synchronous belt, the upper part of the right side of the positioning screw rod is fixedly connected with the rope wheel, the rope wheel is wound with the positioning rope, the tail end of the positioning rope is fixedly connected with a positioning slide block, the left side and the right side of the positioning screw rod are in threaded connection with the soft rope limiting frame for limiting the culture dish, the reset belt assembly is connected between the lower part of the right side of the positioning screw rod and the sealed bottom box and consists of two belt pulleys and a belt, one belt pulley is installed at the lower part of the right side of the positioning screw rod, the other belt pulley is rotatably installed at the lower part of the sealed bottom box, the belt is wound between the two belt pulleys, and the reset spring is connected between the belt of the reset belt assembly and the sealed bottom box.
Further, the guide mechanism comprises a guide pipe, a positioning seat, a column positioning transverse plate, a connecting vertical frame, a column disc and a speed reducing belt component, the guide pipe for uniform exhaust is rotationally arranged on the left side and the right side of the air duct, the positioning seat is fixedly connected to the rear portion of the guide pipe on the left side and the right side, the column disc is rotationally arranged on the upper side of the rear portion of the sealed bottom box, the connecting vertical frame is slidably connected with the column disc, the column positioning transverse plate is fixedly connected to the connecting vertical frame, the column positioning transverse plate is slidably connected with the left side and the right side positioning seat, the speed reducing belt component is connected between the rear portion of the column disc and the front portion of the driving shaft, the speed reducing belt component consists of two belt pulleys and a belt, one belt pulley is arranged at the rear portion of the column disc, the other belt pulley is arranged at the front portion of the driving shaft, and the belt is wound between the two belt pulleys.
Further, sealing mechanism for avoiding air reflux is still including being used for, sealing mechanism is including sealing the end cover, one-way airtight board, guide frame and positioning spring reset, and sealing end cover bottom rigid coupling has sealing end cover, and sealing end cover right part rotation is equipped with the one-way airtight board that is used for avoiding air reflux, and sealing end cover outer bottom right side rigid coupling has the guide frame that resets, and the guide frame that resets and one-way airtight board sliding connection have positioning spring evenly spaced rigid coupling between guide frame that resets and the one-way airtight board.
Further, the light shielding mechanism comprises a light shielding plate and hinge joints, wherein the hinge joints are fixedly connected on the left side and the right side of the rear side of the outer top of the sealed bottom box in a bilateral symmetry manner, and the light shielding plate for shielding light is fixedly connected between the hinge joints on the left side and the right side.
Further, the sealing cabinet door also comprises a stressed holding rod, and the middle part of the front side surface of the sealing cabinet door is fixedly connected with two stressed holding rods.
The beneficial effects of the invention are as follows:
1. According to the invention, the culture dish filled with stem cells is placed on the supporting tray, the sealing cabinet door is pulled to swing backwards for resetting, the positioning sliding block moves rightwards for resetting to enable the positioning rope to be loosened, the right positioning screw rod is driven by the resetting belt component to rotate forwards for resetting due to the action of the resetting spring, and the soft rope limiting frame also moves downwards for resetting to limit and fix the culture dish, so that people can conveniently fix the culture dish.
2. Under the effect of guiding mechanism, the left and right sides positioning seat swing about drive left and right sides stand pipe swing about respectively, and left and right sides stand pipe swing about making the even blowout of air current, so, can make the even discharge of air current.
3. Under sealing mechanism's effect, the unidirectional closure board opens and shuts through the positioning spring self-adaptation and ventilates to guarantee to effectively carry out unidirectional water conservancy diversion, prevent that outside air from can flowing backward into sealed base box, so, avoid guaranteeing the unidirectional flow of air current.
Drawings
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a schematic view of a first partially cut-away structure of the present invention.
Fig. 3 is a schematic view of a partially cut-away structure of the ventilation mechanism of the present invention.
Fig. 4 is a schematic view of a first partially cut-away configuration of the locking mechanism of the present invention.
Fig. 5 is a schematic view of a second partially cut-away configuration of the locking mechanism of the present invention.
Fig. 6 is a schematic view of a second partially cut-away structure of the present invention.
Fig. 7 is a schematic view of a first partially cut-away configuration of the spacing mechanism of the present invention.
Fig. 8 is a schematic view of a second partially cut-away configuration of the spacing mechanism of the present invention.
Fig. 9 is a schematic view of a third partial cross-sectional structure of the spacing mechanism of the present invention.
Fig. 10 is a schematic view of a first partially cut-away configuration of the guide mechanism of the present invention.
Fig. 11 is a schematic view of a second partially cut-away configuration of the guide mechanism of the present invention.
Fig. 12 is a schematic view of a third partial cross-sectional structure of the present invention.
Fig. 13 is a schematic view of a partially cut-away structure of the sealing mechanism of the present invention.
Fig. 14 is a schematic view of a partially cut-away structure of a shading mechanism of the present invention.
Part names and serial numbers in the figure: the door assembly comprises a 1-sealing bottom box, a 2-supporting bottom frame, a 3-observing window, a 4-sealing cabinet door, a 41-stressed holding rod, a 42-control screen, a 5-bearing tray, a 6-ventilation mechanism, a 61-air duct, a 62-constant temperature cover, a 63-servo motor, a 64-driving shaft, a 65-fan, a 66-air inlet pipe, a 7-locking mechanism, a 71-locking plate, a 72-positioning sliding block, a 73-positioning magnetic rod, a 74-hinging connecting rod, a 75-sliding locking plate, a 8-limiting mechanism, a 81-positioning rope, a 82-positioning lead screw, a 83-rope, a 86-rope limiting frame, a 87-resetting belt assembly, a 88-resetting spring, a 9-guiding mechanism, a 91-guiding pipe, a 92-positioning seat, a 93-pillar positioning transverse plate, a 94-connecting vertical frame, a 95-pillar disc, a 96-decelerating belt assembly, a 10-sealing mechanism, a 101-sealing bottom cover, a 102-unidirectional sealing plate, a 103-resetting guide frame, a 104-positioning spring, a 11-shading mechanism, a 111-shading plate and a 112-hinging.
Detailed Description
The technical scheme of the invention is further described below with reference to the accompanying drawings.
Example 1
The utility model provides a stem cell's culture apparatus, as shown in fig. 1-5, including sealed underframe 1, support underframe 2, the observation window 3, sealed cabinet door 4, atress holding rod 41, control panel 42, hold and put tray 5, ventilation mechanism 6 and locking mechanical system 7, sealed underframe 1 lower part rigid coupling has support underframe 2, sealed underframe 1 upper portion embedding is equipped with observation window 3, sealed underframe 1 front portion left side rotation is equipped with sealed cabinet door 4, sealed cabinet door 4 leading flank middle part rigid coupling has two atress holding rods 41, be equipped with control panel 42 in the middle of the sealed cabinet door 4 left part, sealed underframe 1 medial surface lower part rigid coupling has holds and puts tray 5, be provided with ventilation mechanism 6 on the sealed underframe 1, ventilation mechanism 6 can be used to be used for discharging into sealed underframe 1 with hot air, be provided with locking mechanical system 7 between sealed cabinet door 4 and the sealed underframe 1, locking mechanical system 7 can be used to carry out the locking to sealed cabinet door 4.
As shown in fig. 2 and 3, the ventilation mechanism 6 includes an air duct 61, a constant temperature bottom cover 62, a servo motor 63, a driving shaft 64, a fan 65 and an air inlet pipe 66, the air duct 61 is arranged at the upper part of the sealed bottom box 1, the constant temperature bottom cover 62 is fixedly connected at the lower part of the outer rear side surface of the sealed bottom box 1, the servo motor 63 is fixedly connected at the middle part of the constant temperature bottom cover 62, the control screen 42 is electrically connected with the servo motor 63, the driving shaft 64 is connected on the output shaft of the servo motor 63, the fan 65 is rotatably arranged at the left side and the right side of the constant temperature bottom cover 62, the fan 65 at the left side and the right side are all driven with the driving shaft 64 through synchronous belts, and four air inlet pipes 66 are connected between the air duct 61 and the constant temperature bottom cover 62.
As shown in fig. 2, 4 and 5, the locking mechanism 7 comprises a locking plate 71, a positioning sliding block 72, a positioning magnetic rod 73, a hinged connecting rod 74 and a sliding locking block 75, wherein the locking plate 71 is symmetrically arranged on the right part of the sealed cabinet door 4 in a vertically rotating mode, the sliding locking block 75 is symmetrically arranged on the front side of the right part of the sealed bottom box 1 in a vertically sliding mode, the sliding locking blocks 75 on the upper side and the lower side are respectively contacted with the locking plate 71 on the upper side and the lower side, the positioning magnetic rod 73 is fixedly connected on the upper side of the front part of the sealed bottom box 1, the positioning sliding block 72 is arranged on the positioning magnetic rod 73 in a sliding mode, and the hinged connecting rod 74 is rotatably arranged between the positioning sliding block 72 and the sealed cabinet door 4.
Firstly, an operator pulls the upper and lower sliding locking blocks 75 to move backwards, the upper and lower sliding locking blocks 75 move backwards to stop limiting the upper and lower locking plates 71 respectively, then, the person pulls the sealing cabinet door 4 to swing forwards through the stressed holding rod 41, the sealing cabinet door 4 swings forwards to drive the positioning sliding blocks 72 to move leftwards through the hinged connecting rods 74, then, the person can place a culture dish containing stem cells on the supporting tray 5, pull the sealing cabinet door 4 to swing backwards to reset, then, the upper and lower sliding locking blocks 75 are inserted into the upper and lower locking plates 71 respectively, the upper and lower sliding locking blocks 75 limit the locking plates 71 respectively, the sealing cabinet door 4 is limited, then, the person starts the servo motor 63 to work through the control screen 42, the servo motor 63 drives the driving shaft 64 to rotate, the driving shaft 64 drives the left and right fans 65 to rotate through synchronous belt transmission, the left and right fans 65 rotate to generate air flow, and as the constant temperature bottom cover 62 generates certain heat, the air flow is heated and then is discharged into the air duct 61 through the air inlet pipe 66, so that the air duct 61 discharges hot air to cultivate stem cells, the observation window 3 is beneficial to operators to observe, then the air flow is discharged from the bottom of the sealed bottom box 1, the operation is repeated, the stem cells can be continuously cultivated, after the stem cell cultivation is completed, the servo motor 63 is closed, the driving shaft 64 stops driving the left and right fans 65 to rotate through synchronous belt transmission, and the left and right fans 65 also stop rotating.
Example 2
On the basis of the embodiment 1, as shown in fig. 6-9, the device further comprises a limiting mechanism 8, the limiting mechanism 8 comprises a positioning rope 81, a positioning screw rod 82, a rope pulley 83, a soft rope limiting frame 86, a reset belt assembly 87 and a reset spring 88, the middle parts of the left side and the right side of the sealed bottom box 1 are rotatably provided with the positioning screw rod 82, the positioning screw rods 82 on the left side and the right side are transmitted through synchronous belts, the rope pulley 83 is fixedly connected with the upper part of the right positioning screw rod 82, the rope pulley 83 is wound with the positioning rope 81, the tail end of the positioning rope 81 is fixedly connected with the positioning sliding block 72, the soft rope limiting frame 86 is connected between the left side and the right side positioning screw rods 82 in a threaded manner, the soft rope limiting frame 86 can limit a culture dish, a reset belt assembly 87 is connected between the lower part of the right positioning screw rod 82 and the sealed bottom box 1, the reset belt assembly 87 is composed of two belt pulleys and a belt, one belt pulley is rotatably arranged at the lower part of the right positioning screw rod 82, the other belt pulley is rotatably arranged at the lower part of the sealed bottom box 1, the belt is wound between the two belt pulleys, and the reset spring 88 is connected between the belt of the reset belt assembly 87 and the sealed bottom box 1.
As shown in fig. 6, 10 and 11, the air guide device further comprises a guide mechanism 9, the guide mechanism 9 comprises a guide tube 91, a positioning seat 92, a column positioning transverse plate 93, a connecting vertical frame 94, a column disc 95 and a speed reducing belt assembly 96, the guide tube 91 is rotatably arranged at the left side and the right side of the air guide tube 61, the guide tube 91 can uniformly discharge air flow, the positioning seat 92 is fixedly connected at the rear part of the guide tube 91 at the left side and the right side, the column disc 95 is rotatably arranged at the middle upper side of the rear part of the sealed bottom box 1, the connecting vertical frame 94 is slidably connected with the column disc 95 at the upper side of the rear part of the sealed bottom box 1, the column positioning transverse plate 93 is fixedly connected with the column positioning transverse plate 93 on the connecting vertical frame 94, the speed reducing belt assembly 96 is connected between the rear part of the column disc 95 and the front part of the driving shaft 64, the speed reducing belt assembly 96 consists of two belt pulleys and a belt, one belt pulley is mounted at the rear part of the column disc 95, and the other belt pulley is mounted at the front part of the driving shaft 64 and the belt is wound between the two belt pulleys.
When the sealing cabinet door 4 is pulled to swing forwards, the sealing cabinet door 4 swings forwards, the positioning slide block 72 is driven to move leftwards through the hinged connecting rod 74, the positioning slide block 72 moves leftwards, the rope pulley 83 is driven to rotate reversely through the positioning rope 81, the rope pulley 83 rotates reversely to drive the right positioning screw rod 82 to rotate reversely, the right positioning screw rod 82 rotates reversely to drive the reset belt assembly 87 to rotate reversely, the reset spring 88 is compressed, the positioning screw rods 82 on the left side and the right side rotate synchronously through synchronous belt transmission, the soft rope limiting frame 86 is driven to move upwards by the left positioning screw rod 82 and the right positioning screw rod 82 to rotate reversely, then the culture dish containing stem cells is placed on the supporting tray 5, the sealing cabinet door 4 is pulled to swing backwards to reset, the positioning slide block 72 moves rightwards to reset the positioning rope 81 to be relaxed, and the reset belt assembly 87 drives the right positioning screw rod 82 to rotate forwards to reset due to the action of the reset spring 88, and the soft rope limiting frame 86 moves downwards to reset to limit and fix the culture dish, so that the culture dish can be conveniently limited.
When the servo motor 63 works, the driving shaft 64 rotates to drive the speed reducing belt assembly 96 to rotate, the speed reducing belt assembly 96 rotates to drive the column disc 95 to rotate, the column disc 95 rotates to drive the connecting stand 94 to move left and right, the connecting stand 94 moves left and right to drive the column positioning transverse plate 93 to move left and right, the column positioning transverse plate 93 moves left and right to drive the left and right side positioning seats 92 to swing left and right, the left and right side positioning seats 92 swing left and right to drive the left and right side guide pipes 91 to swing left and right respectively, the left and right side guide pipes 91 swing left and right to enable air flow to be evenly blown out, after stem cell culture is completed, the servo motor 63 is closed, the driving shaft 64 stops driving the column disc 95 to rotate through the speed reducing belt assembly 96, and the left and right side guide pipes 91 stop swinging left and right, so that air flow can be evenly discharged.
Example 3
On the basis of embodiment 1 and embodiment 2, as shown in fig. 12 and 13, the sealing mechanism 10 is further comprised of a sealing mechanism 10, the sealing mechanism 10 comprises a sealing bottom cover 101, a unidirectional sealing plate 102, a reset guide frame 103 and a positioning spring 104, the outer bottom of the sealing bottom box 1 is fixedly connected with the sealing bottom cover 101, the unidirectional sealing plate 102 is rotatably arranged on the right part of the sealing bottom cover 101, the reset guide frame 103 is fixedly connected on the right side of the outer bottom of the sealing bottom box 1, the reset guide frame 103 is in sliding connection with the unidirectional sealing plate 102, the positioning spring 104 is fixedly connected between the reset guide frame 103 and the unidirectional sealing plate 102 at uniform intervals, and the unidirectional sealing plate 102 is opened and closed in a self-adaptive manner through the positioning spring 104 so as to ensure that unidirectional air flow guiding is effectively carried out.
As shown in fig. 12 and 14, the device further comprises a light shielding mechanism 11, the light shielding mechanism 11 comprises a light shielding plate 111 and hinge hinges 112, the hinge hinges 112 are fixedly connected on the rear side of the outer top of the sealed bottom box 1 in a bilateral symmetry manner, the light shielding plate 111 is fixedly connected between the hinge hinges 112 on the left side and the hinge hinges 112 on the right side, and the light shielding plate 111 can shield the observation window 3 and prevent stem cells from illuminating for a long time.
When people use the device, air flow is discharged from the bottom of the sealed bottom box 1, and the unidirectional sealing plate 102 is opened and closed in a self-adaptive mode through the positioning spring 104 so as to ensure that unidirectional flow guiding is effectively carried out, and prevent external air from flowing backwards into the sealed bottom box 1, so that the air flow can flow unidirectionally.
When people use the device, if stem cells need to be in the environment with weaker light for culturing, people can pull the light shielding plate 111 to cover the observation window 3, long-time illumination of the stem cells is avoided, the culturing effect is influenced, and therefore the light shielding treatment of the stem cells can be conveniently carried out.
The technical principles of the embodiments of the present invention are described above in connection with specific embodiments. The description is only intended to explain the principles of the embodiments of the invention and should not be taken in any way as limiting the scope of the embodiments of the invention. Based on the explanations herein, those skilled in the art will recognize other embodiments of the present invention without undue burden, and those ways that are within the scope of the present invention.
Claims (6)
1. The utility model provides a stem cell's culture apparatus, including sealed underframe (1), support underframe (2), observation window (3), sealed cabinet door (4), control screen (42) and hold and put tray (5), sealed underframe (1) lower part rigid coupling has support underframe (2), sealed underframe (1) upper portion embedded be equipped with observation window (3), sealed underframe (1) anterior left side rotation is equipped with sealed cabinet door (4), be equipped with control screen (42) in the middle of sealed cabinet door (4) left part, sealed underframe (1) medial surface lower part rigid coupling has holds and puts tray (5), characterized by still including ventilation mechanism (6) and locking mechanical system (7), be provided with on sealed underframe (1) and be used for the ventilation mechanism (6) of air-intake, be provided with between sealed cabinet door (4) and sealed underframe (1) locking mechanical system (7) that are used for locking the door plant; the locking mechanism (7) comprises a locking plate (71), a positioning sliding block (72), a positioning magnetic rod (73), a hinged connecting rod (74) and a sliding locking block (75), wherein the locking plate (71) is symmetrically arranged on the right part of the sealing cabinet door (4) in a vertically symmetrical and rotating mode, the sliding locking block (75) is symmetrically arranged on the front side of the right part of the sealing bottom box (1) in a vertically sliding mode, the sliding locking blocks (75) on the upper side and the lower side are respectively contacted with the locking plates (71) on the upper side and the lower side, the positioning magnetic rod (73) is fixedly connected on the upper side of the front part of the sealing bottom box (1), the positioning sliding block (72) is arranged on the positioning magnetic rod (73) in a sliding mode, and the hinged connecting rod (74) is rotatably arranged between the positioning sliding block (72) and the sealing cabinet door (4); still including being used for spacing stop gear (8) to the culture dish, stop gear (8) are including locating rope (81), location lead screw (82), rope sheave (83), rope spacing (86), reset belt subassembly (87) and reset spring (88), sealed base box (1) left and right sides middle part all rotates and is equipped with location lead screw (82), through the hold-in range transmission between left and right sides location lead screw (82), right side location lead screw (82) upper portion rigid coupling has rope sheave (83), around having locating rope (81) on rope sheave (83), locating rope (81) tail end and location slider (72) fixed connection, threaded connection has between left and right sides location lead screw (82) and is used for spacing rope spacing (86) to the culture dish, be connected with reset belt subassembly (87) between right side location lead screw (82) lower part and the sealed base box (1), reset belt subassembly (87) are by two belt pulleys and belt constitution, one belt pulley is installed in right side location lead screw (82) lower part, another belt pulley rotation is installed in sealed base box (1) lower part, the belt pulley is wound between two reset belt subassembly (87) and reset spring (1).
2. The stem cell incubator of claim 1, wherein the ventilation mechanism (6) comprises an air duct (61), a constant temperature bottom cover (62), a servo motor (63), a driving shaft (64), a fan (65) and air inlet pipes (66), the air duct (61) is arranged on the upper portion of the sealed bottom box (1), the constant temperature bottom cover (62) is fixedly connected to the lower portion of the outer rear side face of the sealed bottom box (1), the servo motor (63) is fixedly connected to the middle portion of the constant temperature bottom cover (62), the control screen (42) is electrically connected with the servo motor (63), the driving shaft (64) is connected to an output shaft of the servo motor (63), the fan (65) is rotatably arranged on the left side and the right side of the constant temperature bottom cover (62), the fans (65) and the driving shaft (64) are in transmission through synchronous belts, and four air inlet pipes (66) are connected between the air duct (61) and the constant temperature bottom cover (62).
3. The stem cell incubator of claim 2, further comprising a guide mechanism (9) for uniform exhaust, wherein the guide mechanism (9) comprises a guide tube (91), a positioning seat (92), a column positioning cross plate (93), a connecting stand (94), a column positioning cross plate (95) and a speed reducing belt assembly (96), the left side and the right side of the air guide tube (61) are both rotatably provided with the guide tube (91) for uniform exhaust, the rear parts of the guide tube (91) on the left side and the right side are both fixedly connected with the positioning seat (92), the upper side in the rear part of the sealing bottom box (1) is rotatably provided with the column positioning cross plate (95), the upper side of the rear part of the sealing bottom box (1) is slidably provided with the connecting stand (94), the column positioning cross plate (93) is fixedly connected with the column positioning cross plate (93), the speed reducing belt assembly (96) is connected between the rear part of the column positioning cross plate (93) and the front part of the driving shaft (64), the speed reducing belt assembly (96) is arranged between the rear part of the column positioning cross plate (95) and the front part of the driving shaft (64), and the driving shaft (64) is arranged between the two belt pulleys.
4. A stem cell culture apparatus according to claim 3, further comprising a sealing mechanism (10) for preventing air from flowing back, wherein the sealing mechanism (10) comprises a sealing bottom cover (101), a unidirectional sealing plate (102), a reset guide frame (103) and a positioning spring (104), the sealing bottom cover (101) is fixedly connected to the outer bottom of the sealing bottom case (1), the unidirectional sealing plate (102) for preventing air from flowing back is rotatably arranged on the right part of the sealing bottom cover (101), the reset guide frame (103) is fixedly connected to the right side of the outer bottom of the sealing bottom case (1), the reset guide frame (103) is slidably connected with the unidirectional sealing plate (102), and the positioning spring (104) is fixedly connected between the reset guide frame (103) and the unidirectional sealing plate (102) at uniform intervals.
5. The stem cell incubator of claim 4, further comprising a light shielding mechanism (11) for shielding light, wherein the light shielding mechanism (11) comprises a light shielding plate (111) and a hinge (112), the hinge (112) is symmetrically and fixedly connected to the rear side of the outer top of the sealed bottom case (1), and the light shielding plate (111) for shielding light is fixedly connected between the hinge (112) on the left side and the hinge (112) on the right side.
6. The stem cell incubator of claim 5, further comprising a stressed holding rod (41), wherein the middle part of the front side surface of the sealing cabinet door (4) is fixedly connected with two stressed holding rods (41).
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| CN202210959041.8A CN115287188B (en) | 2022-08-11 | 2022-08-11 | Stem cell culture instrument |
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| CN214690835U (en) * | 2021-03-17 | 2021-11-12 | 河南和泽干细胞基因工程有限公司 | Portable stem cell temporary storage device |
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| JP2012147678A (en) * | 2009-05-14 | 2012-08-09 | Otake:Kk | Multiple culture apparatus for single sample |
| CN212833823U (en) * | 2020-06-08 | 2021-03-30 | 浙江海福生物科技有限责任公司 | Stem cell amplification device |
| CN111733075B (en) * | 2020-07-03 | 2023-03-14 | 上海中成诺舟生物科技有限公司 | Stem cell culture device |
| CN213232318U (en) * | 2020-07-31 | 2021-05-18 | 江西新纪元生物医学技术有限公司 | Convenient fixed stem cell culture appearance |
| CN214881592U (en) * | 2021-07-09 | 2021-11-26 | 广州苿莱生物科技有限公司 | Constant-temperature culture and storage device for adult stem cells |
| CN215560342U (en) * | 2021-07-19 | 2022-01-18 | 浙江立尚环保科技有限公司 | Novel bacteria culture device |
| CN114657065A (en) * | 2021-09-07 | 2022-06-24 | 河南茵特赛尔生物技术有限公司 | Stem cell culture device and stem cell culture method |
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Address after: 514700 Group 4, Lantian Village, Meinan Town, Meixian County, Meizhou City, Guangdong Province Applicant after: Wu Yuanfen Address before: 518100 District 44, Anle Community, Xin'an Street, Bao'an District, Shenzhen, Guangdong Applicant before: Wu Yuanfen |
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Effective date of registration: 20240613 Address after: 200000 building 4, No. 686, Nanfeng Road, Fengcheng Town, Fengxian District, Shanghai Applicant after: Zhongyou Kangheng (Shanghai) Stem Cell Co.,Ltd. Country or region after: China Address before: 514700 Group 4, Lantian Village, Meinan Town, Meixian County, Meizhou City, Guangdong Province Applicant before: Wu Yuanfen Country or region before: China |
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