CN219490037U

CN219490037U - Cell culture apparatus

Info

Publication number: CN219490037U
Application number: CN202320856647.9U
Authority: CN
Inventors: 仲强
Original assignee: Shandong Zhisheng Biotechnology Co ltd
Current assignee: Shandong Zhisheng Biotechnology Co ltd
Priority date: 2023-04-18
Filing date: 2023-04-18
Publication date: 2023-08-08
Anticipated expiration: 2033-04-18

Abstract

The utility model belongs to the technical field of cell culture, in particular to a cell culture device, which aims at the problems that when the existing cells are cultured, oxygen is difficult to uniformly meet the use requirement of all cells in aerobic culture due to large culture quantity, the culture states of all cells are different, and the cultured cells are inconvenient to take and place in an incubator. The utility model has simple operation and convenient use, can conveniently take and put a large number of cells in culture, and simultaneously can conveniently inject oxygen into a plurality of layers of cells, thereby being convenient for people to use.

Description

Cell culture apparatus

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture device.

Background

Cell culture refers to a method for simulating in vitro in vivo environment (asepsis, proper temperature, pH value, a certain nutrition condition and the like) to enable the cells to survive, grow, reproduce and maintain main structures and functions, and is also called a cell cloning technology, a normal term in biology is a cell culture technology, the cell culture is an essential process for the whole bioengineering technology or one of the bioengineering technologies, the cell culture is a large-scale cloning of cells, the cell culture technology can be a simple single cell or a few differentiated multiple cells from one cell through a large-scale culture, the cloning technology is an essential link, and the cell culture is a cloning of cells. Cell culture techniques are important and commonly used techniques in cell biology research methods, by which a large number of cells can be obtained, and by which signal transduction of cells, anabolism of cells, growth and proliferation of cells, etc. can be studied. The cells need to be cultured aerobically and anaerobically during culturing.

When the existing cells are cultured, because the quantity of the cells is large, oxygen is difficult to uniformly meet the use requirement of all the cells in aerobic culture, so that the culture states of all the cells are different, and the cultured cells are inconvenient to take and place in an incubator, so we propose a cell culture device.

Disclosure of Invention

The utility model aims to solve the defects that when the existing cells are cultured, the oxygen is difficult to uniformly meet the use requirement of all cells in aerobic culture due to the large quantity of the cells, the culture states of the cells are different, and the cultured cells are inconvenient to take and place in an incubator.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model provides a cell culture device, includes the incubator, the top of incubator is equipped with the apron, sliding connection has the cultivation frame on the inner wall of incubator, install a plurality of lamellas on the cultivation frame, all seted up a plurality of cultivation holes on cultivation frame and the plywood, be equipped with oxygen injection mechanism on the apron, be equipped with elevating system in the incubator, elevating system is connected with cultivation frame transmission, fixed mounting has the positioning seat that two symmetries set up on the inner wall of incubator, and fixed mounting has the motor on one of two positioning seats, the output shaft and the lift transmission of motor are connected, sealed pad is installed to the inboard of apron.

Preferably, the oxygen injection mechanism comprises an oxygen generator, the oxygen generator is arranged on the cover plate, an air duct is arranged on the oxygen generator, a plurality of branch pipes are arranged on the air duct, and a plurality of through holes are formed in the branch pipes.

Preferably, the elevating system includes two screws that the symmetry set up, and two screws rotate and install on the bottom inner wall of incubator, and the screw is rotated with the positioning seat that corresponds and is connected, and the output shaft of motor and the screw fixed connection who corresponds, two screw drive are connected, and the both sides of cultivateing the frame are all seted up flutedly, and fixed mounting has the removal seat on the inner wall of recess, removes seat and screw threaded connection who corresponds.

Preferably, the screw is fixedly sleeved with a belt pulley, and the two belt pulleys are connected with the same belt in a transmission way.

Preferably, the top fixed mounting of cultivateing the frame has two pull rods of symmetry setting, and the top fixed mounting of pull rod has the baffle, and the bottom fixed mounting of baffle has a top spring, the bottom and the apron fixed connection of top spring, fixed mounting has the locating plate on the pull rod.

Compared with the prior art, the utility model has the advantages that:

according to the scheme, due to the arrangement of the oxygenerator and the air guide pipe and the effects of the plurality of branch pipes and the through holes, oxygen manufactured by the oxygenerator can be rapidly and uniformly distributed between each laminate and the through holes, so that oxygen can be supplemented for each cell;

because the transmission of two belt pulleys and belt is connected, and screw rod and the threaded connection who removes the seat for the output shaft of motor can drive two screw rods and rotate simultaneously, and then can drive the culture rack and remove, and then is convenient for shift out the incubator and get and put.

The utility model has simple operation and convenient use, can conveniently take and put a large number of cells in culture, and simultaneously can conveniently inject oxygen into a plurality of layers of cells, thereby being convenient for people to use.

Drawings

FIG. 1 is a schematic diagram of a cell culture apparatus according to the present utility model;

FIG. 2 is a schematic diagram showing the cross-sectional structure of a culture rack and a laminate of a cell culture apparatus according to the present utility model;

FIG. 3 is a schematic diagram showing the sectional structures of the air duct and the branched pipes of the cell culture apparatus according to the present utility model.

In the figure: 1. an incubator; 2. a cover plate; 3. a culture rack; 4. a laminate; 5. a culture well; 6. a groove; 7. a movable seat; 8. a screw; 9. a positioning seat; 10. a motor; 11. a pull rod; 12. a baffle; 13. a top spring; 14. a positioning plate; 15. an oxygenerator; 16. an air duct; 17. a branch pipe; 18. a through hole; 19. an electric heating plate; 20. a temperature sensor; 21. a controller; 22. and a sealing gasket.

Detailed Description

The technical solutions of the present embodiment will be clearly and completely described below with reference to the drawings in the present embodiment, and it is apparent that the described embodiments are only some embodiments of the present embodiment, not all embodiments.

Example 1

Referring to fig. 1-3, a cell culture device, including incubator 1, the top of incubator 1 is equipped with apron 2, sliding connection has cultivate frame 3 on the inner wall of incubator 1, install a plurality of plywood 4 on cultivate frame 3, a plurality of cultivation holes 5 have all been seted up on cultivate frame 3 and the plywood 4, be equipped with oxygen injection mechanism on the apron 2, be equipped with elevating system in the incubator 1, elevating system is connected with cultivate frame 3 transmission, fixed mounting has two locating seat 9 of symmetry setting on the inner wall of incubator 1, fixed mounting has motor 10 on one locating seat 9 in two locating seats 9, the output shaft and the lift transmission of motor 10 are connected, sealing gasket 22 is installed to the inboard of apron 2.

In this embodiment, the oxygen injection mechanism includes the oxygenerator 15, and the oxygenerator 15 is installed on apron 2, installs air duct 16 on the oxygenerator 15, installs a plurality of minutes pipes 17 on the air duct 16, has seted up a plurality of through-holes 18 on minutes pipes 17, and the setting of oxygenerator 15 and air duct 16, and a plurality of minutes pipes 17 and the effect of through-holes 18 for the oxygen that oxygenerator 15 was made can evenly distributed fast between each plywood 4 and through-holes 18, thereby can carry out oxygen to each cell and supplement.

In this embodiment, elevating system includes the screw rod 8 that two symmetries set up, two screw rods 8 rotate and install on incubator 1's bottom inner wall, screw rod 8 rotates with the positioning seat 9 that corresponds to be connected, motor 10's output shaft and the screw rod 8 fixed connection that corresponds, two screw rod 8 transmission are connected, the recess 6 has all been seted up to the both sides of cultivateing frame 3, fixed mounting has on the inner wall of recess 6 and removes seat 7, remove seat 7 and the screw rod 8 threaded connection that corresponds, fixed cover is equipped with the belt pulley on the screw rod 8, the transmission is connected with same belt on two belt pulleys, motor 10's output shaft passes through the transmission of two belt pulleys and belt to can drive two screw rods 8 and rotate simultaneously, pivoted screw rod 8 moves through the threaded connection drive cultivateing frame 3 with remove seat 7, and then can be convenient for get the cell of placing of cultivateing.

In this embodiment, the top fixed mounting of cultivateing frame 3 has two pull rods 11 that symmetry set up, and the top fixed mounting of pull rod 11 has baffle 12, and the bottom fixed mounting of baffle 12 has top spring 13, and the bottom and the apron 2 fixed connection of top spring 13 have locating plate 14 on the pull rod 11, and the cultivateing frame 3 of removal can drive apron 2 through pull rod 11 and locating plate 14 and remove to can press apron 2 through top spring 13 for apron 2 seals incubator 1.

The theory of operation, when need get the cell and put, starter motor 10 switch, the output shaft of motor 10 is connected with the transmission of belt through two belt pulleys, thereby can drive two screw rods 8 and rotate simultaneously, pivoted screw rod 8 is through moving the threaded connection with remove seat 7 and drive cultivate frame 3 and remove, and then can be convenient for get the cell of cultivating and put, the cultivation frame 3 of removal can drive apron 2 through pull rod 11 with locating plate 14 and remove, and can press apron 2 through top spring 13, make apron 2 seal incubator 1, oxygenerator 15 and the setting of air duct 16 simultaneously, and the effect of a plurality of being in charge of 17 and through-hole 18, make the oxygen that oxygenerator 15 made can be quick evenly distributed between each plywood 4 and through-hole 18, thereby can carry out oxygen to each cell and supplement.

Example two

The difference between the second embodiment and the first embodiment is that the electric heating plate 19 is installed on the inner wall of the bottom of the incubator 1, the temperature sensor 20 is installed on the inner wall of one side of the incubator 1, the controller 21 is installed on one side of the incubator 1, and the arrangement of the electric heating plate 19 and the temperature sensor 20 can facilitate the control of the temperature in the incubator 1 and the monitoring through the controller 21.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art will be able to apply equally to the technical solution of the present utility model and the inventive concept thereof, within the scope of the present utility model.

Claims

1. The utility model provides a cell culture device, includes incubator (1), its characterized in that, the top of incubator (1) is equipped with apron (2), sliding connection has cultivate frame (3) on the inner wall of incubator (1), install a plurality of plywood (4) on cultivateing frame (3), cultivate all offered a plurality of cultivation holes (5) on frame (3) and plywood (4), be equipped with oxygen injection mechanism on apron (2), be equipped with elevating system in incubator (1), elevating system is connected with cultivateing frame (3) transmission, fixed mounting has positioning seat (9) of two symmetry settings on the inner wall of incubator (1), fixed mounting has motor (10) on one positioning seat (9) in two positioning seats (9), the output shaft and the lift transmission of motor (10) are connected, sealed pad (22) are installed to the inboard of apron (2).

2. The cell culture apparatus according to claim 1, wherein the oxygen injection mechanism comprises an oxygen generator (15), the oxygen generator (15) is mounted on the cover plate (2), an air duct (16) is mounted on the oxygen generator (15), a plurality of branch pipes (17) are mounted on the air duct (16), and a plurality of through holes (18) are formed in the branch pipes (17).

3. The cell culture apparatus according to claim 1, wherein the lifting mechanism comprises two symmetrically arranged screws (8), the two screws (8) are rotatably mounted on the inner wall of the bottom of the incubator (1), the screws (8) are rotatably connected with corresponding positioning seats (9), the output shaft of the motor (10) is fixedly connected with the corresponding screws (8), the two screws (8) are in transmission connection, grooves (6) are formed in two sides of the culture rack (3), movable seats (7) are fixedly mounted on the inner wall of the grooves (6), and the movable seats (7) are in threaded connection with the corresponding screws (8).

4. A cell culture apparatus according to claim 3, wherein the screw (8) is fixedly sleeved with a pulley, and the two pulleys are in driving connection with the same belt.

5. The cell culture apparatus according to claim 1, wherein two symmetrically arranged pull rods (11) are fixedly arranged at the top of the culture rack (3), a baffle plate (12) is fixedly arranged at the top end of the pull rods (11), a top spring (13) is fixedly arranged at the bottom of the baffle plate (12), the bottom end of the top spring (13) is fixedly connected with the cover plate (2), and a positioning plate (14) is fixedly arranged on the pull rods (11).