CN102061258B - Cell exposed anaerobic environment experiment device - Google Patents
Cell exposed anaerobic environment experiment device Download PDFInfo
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- CN102061258B CN102061258B CN201010538996.3A CN201010538996A CN102061258B CN 102061258 B CN102061258 B CN 102061258B CN 201010538996 A CN201010538996 A CN 201010538996A CN 102061258 B CN102061258 B CN 102061258B
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Abstract
The invention provides a cell exposed anaerobic environment experiment device, which comprises a main air pipe, wherein the air inlet end of the main air pipe is connected with a pure oxygen source and a nitrogen source or other inert gas source, and the air outlet end of the main air pipe is connected with a waste gas treatment device. An air inlet pipe and an air outlet pipe on the main air pipe are simultaneously connected with a cell exposing cavity. A cell membrane plate is arranged between the cell exposing cavity and a cell culture solution cavity, and a warm bath cavity is positioned outside the cell membrane plate. A culture solution supply device is connected with the cell culture solution cavity. The device has the advantages that: an experimental animal is substituted by using an in vitro culture substance in the anaerobic environment experiment to realize optimization and improvement of an experiment method; and anaerobic experiments are directly performed on the culture substances such as target organ cells and the like, so the whole process of the experiments is controlled conveniently, and precise experiment results are obtained. The special cell exposed anaerobic environment experiment device can be widely applied in the fields of medicinal science, public health environmental science, molecular biology, cytology, toxicology and the like.
Description
Technical field
The present invention relates to a kind of cell-exposure oxygen-deficient environment experimental device.
Background technology
In the scientific domains such as present stage biology, environmentalism, diseases prevention and control, medical science, military science, toxicology, anoxic experiment also rests on animal experiment stage.This exists that the laboratory animal quantity needing is many, experiment condition is harsh, animal individual difference causes greatly that experiment effect is not good, the requirement of cost high and animal welfare and the protection aspect of testing etc. is unfavorable for factor seriously restricting going deep into of this type of research.And appearance and the swift and violent development exigence one of cell toxicology research can accurately be controlled and the direct experimental installation of effect.
Summary of the invention
For addressing the above problem, the invention provides a kind of cell-exposure oxygen-deficient environment experimental device, get rid of the interference of other factors in experiment, target organ is directly carried out to anoxic experiment, and can control experiment whole process.
For achieving the above object, technical scheme of the present invention is to provide a kind of cell-exposure oxygen-deficient environment experimental device, comprises that main tracheae air inlet one end is connected with nitrogen or other rare gas element sources of the gas with pure oxygen source of the gas, and the one end of giving vent to anger is connected with emission-control equipment.Inlet pipe on main tracheae exposes chamber with cell with escape pipe simultaneously and is connected.Cell exposes between chamber and cell cultures sap cavity cell lamina membranacea, has temperature to bathe chamber outside it.Cell cultures sap cavity is connected with nutrient solution feeding mechanism.
Described cell lamina membranacea is placed on cell and exposes between chamber and cell cultures sap cavity, cell lamina membranacea upper surface is exposed to cell completely and exposes in chamber, lower surface infiltrates in cell cultures sap cavity, and cell exposure chamber, cell cultures sap cavity and this three part of cell lamina membranacea are all wrapped up completely by temperature bath chamber.
Main tracheae and pure oxygen source of the gas and nitrogen or other rare gas element sources of the gas are connected by the inlet pipe with control damper, on main tracheae, between these two sources of the gas and inlet pipe, tensimeter are housed, and between inlet pipe and escape pipe, control damper are housed.
Described inlet pipe bottom and escape pipe bottom are diffusing opening.
Described temperature is bathed chamber and is connected by conduit and temperature bath control device.
Described cell cultures sap cavity and be equipped with between the nutrient solution feeding mechanism of nutrient solution flow director current stabilization pump is housed, the other end is equipped with cell culture fluid water conservancy diversion delivery pipe.
Its beneficial effect is to substitute laboratory animal with vitro culture thing in anaerobic environment experiment, realizes optimization and the improvement of test method, and the cultures such as target organ cell are directly carried out to anoxic experiment, controls easily experiment whole process, obtains accurate experimental result.Can be widely used in the special cell-exposure oxygen-deficient environment experimental device in each fields such as medical science, public hygienic environment, molecular biology, cytology, toxicology.
Brief description of the drawings
Accompanying drawing is the schematic diagram of structure of the present invention.
In figure:
1, emission-control equipment
2, control damper
3, main tracheae
4, tensimeter
5, pure oxygen source of the gas
6, nitrogen or other rare gas element sources of the gas
7, cell culture fluid water conservancy diversion delivery pipe
8, cell exposes chamber
9, diffusing opening
10, inlet pipe
11, escape pipe
12, conduit
13, temperature is bathed control device
14, nutrient solution feeding mechanism
15, nutrient solution flow director
16, current stabilization pump
17, temperature is bathed chamber
18, cell cultures sap cavity
19, cell lamina membranacea
20, prop up inlet pipe
Embodiment
By reference to the accompanying drawings the present invention is illustrated.
As shown in drawings, main tracheae (3) air inlet one end is connected with nitrogen or other rare gas element sources of the gas (6) with pure oxygen source of the gas (5), the one end of giving vent to anger is connected with emission-control equipment (1), and emission-control equipment (1) can carry out thorough purifying treatment by experiment waste gas and reach safety dumping.Main tracheae (3) and pure oxygen source of the gas (5) and nitrogen or other rare gas element sources of the gas (6) are connected by the inlet pipe (20) with control damper (2), control damper (2) can accurately be controlled the ratio of induction air flow ratio and two kinds of experimental gas, main tracheae (3) is upper is equipped with tensimeter (4) between these two sources of the gas and inlet pipe (10), show at any time the gaseous tension in current main tracheae (3), between inlet pipe (10) and escape pipe (11), control damper (2) is housed, regulates at any time the size of air-flow.Inlet pipe (10) on main tracheae (3) exposes chamber (8) with cell with escape pipe (11) simultaneously and is connected, and the acting in conjunction that control damper (2) is housed between inlet pipe (10) and escape pipe (11) is issued to the object of accurate control degree of oxygen deficiency.Inlet pipe (10) bottom and escape pipe (11) bottom are diffusing opening, can fully cover micropore lamina membranacea and make cell gaseous expose the abundant exposing cell upper surface of interior gaseous substance in experiment chamber.Cell exposes between chamber (8) and cell cultures sap cavity (18) cell lamina membranacea (19), cell lamina membranacea (19) upper surface is exposed to cell completely and exposes in chamber (8), lower surface infiltrates in cell cultures sap cavity (18), ensures that the required nutrition of Growth of Cells is constantly supplied.Temperature is bathed chamber (17) and is wrapped in whole cell exposure chamber (8), cell lamina membranacea (19) and cell cultures sap cavity (18) outside completely, and bathe control device (13) by conduit (12) and temperature and be connected, the temperature that temperature is bathed in chamber (17) keeps constant.Cell cultures sap cavity (18) is connected with nutrient solution feeding mechanism (14), and current stabilization pump (16) is housed between the two, and the other end is equipped with cell culture fluid water conservancy diversion delivery pipe (7).Nutrient solution flow director (15) is housed on nutrient solution feeding mechanism (14).
When use, first open cell exposure chamber (8) and under aseptic condition, be placed on cell cultures sap cavity (18) with the cell lamina membranacea (19) of experimental cell cultivating, after steadily putting well, cell is exposed to chamber (8) and cover tightly.Then regulate gas flow controller (2) and nutrient solution flow director (15), open nutrient solution feeding mechanism (14) and temperature bath control device (13), open pure oxygen source of the gas (5) and nitrogen or other rare gas element sources of the gas (6).Two kinds of gas imports main tracheae (3) simultaneously and is transported to uniformly cell and exposes the upper surface that spreads in experimental cell in chamber (8) experimental cell is exposed to anaerobic environment experiment.
Claims (4)
1. a cell-exposure oxygen-deficient environment experimental device, it is characterized in that: comprise that main tracheae (3) air inlet one end is connected with nitrogen or other rare gas element sources of the gas (6) with pure oxygen source of the gas (5), the one end of giving vent to anger is connected with emission-control equipment (1), inlet pipe (10) on main tracheae (3) exposes chamber (8) with cell with escape pipe (11) simultaneously and is connected, inlet pipe (10) bottom and escape pipe (11) bottom are diffusing opening (9), cell exposes between chamber (8) and cell cultures sap cavity (18) cell lamina membranacea (19), cell lamina membranacea (19) upper surface is exposed to cell completely and exposes in chamber (8), lower surface infiltrates in cell cultures sap cavity (18), and cell exposes chamber (8), cell cultures sap cavity (18) and this three part of cell lamina membranacea (19) are all bathed chamber (17) by temperature and are wrapped up completely, outside it, there is temperature to bathe chamber (17), cell cultures sap cavity (18) is connected with nutrient solution feeding mechanism (14).
2. cell-exposure oxygen-deficient environment experimental device according to claim 1, it is characterized in that: main tracheae (3) and pure oxygen source of the gas (5) and nitrogen or other rare gas element sources of the gas (6) are connected by the inlet pipe (20) with control damper (2), main tracheae (3) is upper is equipped with tensimeter (4) between these two sources of the gas and inlet pipe (10), between inlet pipe (10) and escape pipe (11), control damper (2) is housed.
3. cell-exposure oxygen-deficient environment experimental device according to claim 1, is characterized in that: described temperature is bathed chamber (17) and is connected by conduit (12) and temperature bath control device (13).
4. cell-exposure oxygen-deficient environment experimental device according to claim 1, it is characterized in that: described cell cultures sap cavity (18) and be equipped with between the nutrient solution feeding mechanism (14) of nutrient solution flow director (15) current stabilization pump (16) is housed, the other end is equipped with cell culture fluid water conservancy diversion delivery pipe (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010538996.3A CN102061258B (en) | 2010-11-11 | 2010-11-11 | Cell exposed anaerobic environment experiment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010538996.3A CN102061258B (en) | 2010-11-11 | 2010-11-11 | Cell exposed anaerobic environment experiment device |
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| Publication Number | Publication Date |
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| CN102061258A CN102061258A (en) | 2011-05-18 |
| CN102061258B true CN102061258B (en) | 2014-07-02 |
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| CN201010538996.3A Expired - Fee Related CN102061258B (en) | 2010-11-11 | 2010-11-11 | Cell exposed anaerobic environment experiment device |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102443534B (en) * | 2011-10-14 | 2013-12-25 | 天津开发区合普工贸有限公司 | Cell intermittent hypoxic simulation experiment equipment |
| CN102352313A (en) * | 2011-10-20 | 2012-02-15 | 天津开发区合普工贸有限公司 | Cellular low-pressure low-oxygen environment simulated experiment system |
| CN102660459A (en) * | 2012-05-14 | 2012-09-12 | 韩宁 | Anaerobic culture box and using method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992019715A1 (en) * | 1991-04-26 | 1992-11-12 | Life Technologies, Inc. | A system and method for delivering oxygen to a cell culture medium |
| CN1876803A (en) * | 2006-04-19 | 2006-12-13 | 东南大学 | Portable hypoxia culture box |
| EP1900804A1 (en) * | 2005-07-01 | 2008-03-19 | Nikon Corporation | Incubator |
| CN201433219Y (en) * | 2009-04-01 | 2010-03-31 | 湖南省天骑医学新技术有限公司 | Cell and bacterium culture box |
-
2010
- 2010-11-11 CN CN201010538996.3A patent/CN102061258B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992019715A1 (en) * | 1991-04-26 | 1992-11-12 | Life Technologies, Inc. | A system and method for delivering oxygen to a cell culture medium |
| EP1900804A1 (en) * | 2005-07-01 | 2008-03-19 | Nikon Corporation | Incubator |
| CN101213289A (en) * | 2005-07-01 | 2008-07-02 | 株式会社尼康 | Incubator |
| CN1876803A (en) * | 2006-04-19 | 2006-12-13 | 东南大学 | Portable hypoxia culture box |
| CN201433219Y (en) * | 2009-04-01 | 2010-03-31 | 湖南省天骑医学新技术有限公司 | Cell and bacterium culture box |
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| CN102061258A (en) | 2011-05-18 |
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Address after: 300020 Tianjin city Nankai District Weishui Road Building 2 room 602 atlas win Applicant after: Tianjin Development Zone Hepu Industry and Trade Co., Ltd. Address before: 300020 Xinjiang Road, Heping District, Tianjin, No. 9 Applicant before: Tianjin Development Zone Hepu Industry and Trade Co., Ltd. |
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Granted publication date: 20140702 Termination date: 20161111 |