CN216712108U - Device for rapidly enriching and culturing anaerobic ammonium oxidation bacteria - Google Patents
Device for rapidly enriching and culturing anaerobic ammonium oxidation bacteria Download PDFInfo
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- CN216712108U CN216712108U CN202123428406.4U CN202123428406U CN216712108U CN 216712108 U CN216712108 U CN 216712108U CN 202123428406 U CN202123428406 U CN 202123428406U CN 216712108 U CN216712108 U CN 216712108U
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Abstract
The utility model discloses a device for rapid enrichment culture of anaerobic ammonium oxidation bacteria, which comprises a culture reactor, a uniform water distribution device, a constant temperature control system and a pH control system, wherein the constant temperature control system comprises a temperature heating device, a temperature controller and a temperature probe which is arranged in the culture reactor in a built-in mode, and the temperature probe and the temperature heating device are connected with the temperature controller; the pH control system comprises a pH controller and a pH probe which is arranged in the culture reactor; the bottom of the culture reactor is provided with an even water distribution device to realize even water distribution on the water passing section; the middle lower part of the culture reactor is a sludge bed reaction area, so that anaerobic ammonia oxidizing bacteria are fully contacted with a reaction substrate; and a three-phase separator is arranged above the sludge bed reaction zone, and the floating sludge is intercepted and sunk through the three-phase separator. The anaerobic ammonium oxidation bacteria are sensitive to temperature and pH, and the temperature and the pH of the reactor can be maintained in proper ranges through the constant temperature control system and the pH control system, so that the stability of the system is ensured.
Description
Technical Field
The utility model relates to the technical field of wastewater treatment and microbial culture, in particular to a device for quickly enriching and culturing anaerobic ammonium oxidation bacteria.
Background
Ammonia nitrogen is an important index for measuring the degree of water pollution and is also one of the control indexes for reducing the emission of water pollutants in China. At present, a widely applied ammonia nitrogen removal mode is mainly a nitrification-denitrification technology, the denitrification efficiency is low, a large amount of carbon sources and alkalinity agents need to be supplemented in actual operation, the sludge production amount is large, and a large burden is brought to actual operation.
Anammox (Anammox) is a green and efficient biological denitrification technology. The anammox bacteria use ammonia as an electron donor to reduce nitrite into nitrogen, and no carbon source or oxygen is needed in the whole reaction process. Has the advantages of saving energy consumption and medicine consumption, high denitrification load, small occupied area and the like, and has wide application prospect.
However, the anammox bacteria grow slowly, have long multiplication time, have high requirements on living environment, are greatly influenced by factors such as substrate concentration, dissolved oxygen, pH, temperature and the like, and have a certain inhibition effect on anammox reaction when the concentration of free ammonia in the system is high. In addition, the starting period of the anammox bacteria in a conventional device is long, the anammox bacteria are easy to run off and not easy to enrich, and the comprehensive problems limit the industrial application of the anammox bacteria. Therefore, the research and the exploration of the device for the rapid enrichment culture of the anaerobic ammonium oxidation bacteria are of great significance.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a device for rapidly enriching and culturing anaerobic ammonium oxidation bacteria, which can accurately monitor and control reaction conditions, prevent sludge loss, improve the enrichment speed of the anaerobic ammonium oxidation bacteria and simultaneously has engineering operability.
In order to achieve the purpose, the application provides a device for rapidly enriching and culturing anaerobic ammonium oxidation bacteria, which comprises a culture reactor, an even water distribution device, a constant temperature control system and a pH control system, wherein the constant temperature control system comprises a temperature heating device, a temperature controller and a temperature probe which is arranged in the culture reactor in a built-in mode, and the temperature probe and the temperature heating device are connected with the temperature controller; the pH control system comprises a pH controller and a pH probe which is arranged in the culture reactor; the bottom of the culture reactor is provided with an even water distribution device to realize even water distribution on the water passing section; the middle lower part of the culture reactor is a sludge bed reaction area, so that anaerobic ammonia oxidizing bacteria are fully contacted with a reaction substrate; and a three-phase separator is arranged above the sludge bed reaction zone, the floating sludge is intercepted and sunk through the three-phase separator, nitrogen generated in the culture reactor is discharged through a top gas outlet, and supernatant liquid after the sludge-water separation enters a water outlet pipe through an overflow weir to be discharged.
Further, the pH controller is connected with a pH regulator medicine barrel through a medicine inlet pump.
Furthermore, the inlet of the culture reactor is connected with the nutrient solution dispensing barrel through a water inlet pump, and an adjusting pipeline is connected between the water inlet pump and the culture reactor and is connected with the drug inlet pump.
Furthermore, the nutrient solution in the nutrient solution dispensing barrel adopts artificially synthesized sewage, and the components of the nutrient solution comprise a nitrogen source, a buffering agent and trace elements. The nitrogen source may be (NH)4)2SO4、NaNO2The buffer mainly adopts KHCO3、KH2PO4The trace elements mainly adopt EDTA and FeSO4、CaCl2、MgSO4NaCl, KCl, etc.
Furthermore, the uniform water distribution device adopts a perforated pattern plate, and a water inlet pipe connected with the perforated pattern plate is provided with a flow controller.
Furthermore, the temperature heating device adopts a heat tracing band which is uniformly arranged on the outer wall of the culture reactor, and heat preservation cotton is laid on the outer layer of the heat tracing band.
Furthermore, the culture reactor is provided with sampling monitoring ports at three different height positions.
Furthermore, a settling zone is arranged between the outer wall of the three-phase separator and the inner wall of the culture reactor, and an air chamber is arranged above the settling zone.
As a further step, a one-way valve is arranged in front of an air outlet at the top of the culture reactor.
As a further step, the culture reactor employs a cylindrical upflow anaerobic sludge blanket.
As a further step, the temperature of the culture reactor is controlled to be maintained at 30-35 ℃, the pH value is 6.8-8.0, the inoculated seed sludge is added into the culture reactor, the sludge concentration after inoculation is not less than 1500mg VSS/L, the total nitrogen concentration of inlet water in the starting stage is controlled to be 50mg/L, and the total nitrogen volume load is controlled to be 0.1kgTN/m3D, after the total nitrogen removal rate is increased to 80%, gradually increasing the total nitrogen volume load to 1.5kgTN/m while maintaining the total nitrogen removal rate not to decrease3·d。
Compared with the prior art, the technical scheme adopted by the utility model has the advantages that: the perforated flower plate is arranged at the bottom of the culture reactor, so that uniform water distribution is realized, the functions of water distribution and hydraulic stirring are realized, the traditional mechanical stirring mode is not needed, the energy consumption is saved in practical application, and the operation is simple and convenient; the upflow anaerobic sludge blanket reactor is adopted, the ascending water flow is fully contacted with anaerobic ammonium oxidation bacteria, the mass transfer effect is better, and the growth rate of microorganisms is improved; the three-phase separator at the top of the culture reactor can efficiently complete mud-water separation, reduce sludge loss and save the subsequent setting of a sedimentation tank; sampling ports are arranged at different longitudinal heights of the culture reactor, and the concentration of a substrate, free ammonia and sludge properties in the reactor are monitored so as to adjust parameters in time; the anaerobic ammonia oxidizing bacteria are sensitive to temperature and pH, and the temperature and the pH of the reactor can be maintained in proper ranges through the constant temperature control system and the pH control system, so that the stable operation of the device is ensured.
Drawings
FIG. 1 is a schematic diagram of a device for rapid enrichment culture of anammox bacteria;
the sequence numbers in the figures illustrate: 1. nutrient solution dispensing barrel, 2 water inlet pump, 3 pH regulator dispensing barrel, 4 medicine inlet pump, 5 pH controller, 6 pH probe, 7 temperature controller, 8 temperature probe, 9 perforated flower plate, 10 sampling monitoring port, 11 air outlet, 12 one-way valve, 13 three-phase separator, 14 air chamber, 15 settling zone, 16 sludge bed reaction zone, 17 overflow weir, 18 heat tracing band.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the application, i.e., the embodiments described are only a subset of, and not all embodiments of the application. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present application without making any creative effort, shall fall within the protection scope of the present application.
Example 1
As shown in fig. 1, the embodiment provides a device for rapid enrichment culture of anammox bacteria, which comprises a culture reactor, a uniform water distribution device, a constant temperature control system and a pH control system; the culture reactor adopts a cylindrical upflow anaerobic sludge blanket form, and a perforated flower plate 9 is arranged at the bottom of the culture reactor, so that nutrient solution enters and is uniformly distributed on a water passing section, and a water inrush area and a dead water area are avoided; the middle lower part of the culture reactor is a sludge bed reaction zone 16, anaerobic ammonia oxidizing bacteria are in a suspension state under the action of ascending water flow and gravity, and a reaction substrate and the anaerobic ammonia oxidizing bacteria are fully contacted and fully reacted in the zone; the upper part of the sludge bed reaction zone is provided with a three-phase separator 13, the floating sludge is intercepted by the three-phase separator 13 and sinks, the generated nitrogen is discharged through a top air outlet 11, and a one-way valve 12 is arranged in front of the air outlet in order to prevent the external air from flowing backwards in an accidental situation; the supernatant fluid after the mud-water separation enters the water outlet pipe through the overflow weir 17 and is discharged.
In the nutrient solution dispensing barrel 1, (NH) can be selected4)2SO4、NaNO2KHCO can be selected to provide the necessary nitrogen source3、KH2PO4EDTA and FeSO can be used as the buffer4、CaCl2、MgSO4NaCl and KCl as trace elements.
In the constant temperature control system, a heat tracing band 18 and heat preservation cotton are uniformly arranged on the outer wall of the culture reactor, a temperature probe 8 is placed in a sludge bed reaction zone 16, and the heat tracing band 18 and the temperature probe 8 are connected with a temperature controller 7, so that the temperature of the sludge bed reaction zone is maintained between 30 ℃ and 35 ℃.
In the pH control system, a pH probe 6 is arranged in a sludge bed reaction zone 16 and is connected with a pH controller 5, and the pH controller 5 simultaneously controls a medicine feeding pump 4 in a linkage manner so as to regulate and control the pH of the sludge bed reaction zone to be maintained between 6.8 and 8.0.
The side wall of the culture reactor and the position of the sludge bed reaction zone are provided with an upper sampling monitoring port 10, a middle sampling monitoring port 10 and a lower sampling monitoring port 10 which are different in height, the reaction zone is sampled periodically, and sludge parameters are monitored so as to adjust the parameters.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the utility model to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the utility model and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the utility model and various alternatives and modifications. It is intended that the scope of the utility model be defined by the claims and their equivalents.
Claims (10)
1. A device for rapid enrichment culture of anaerobic ammonium oxidation bacteria is characterized by comprising a culture reactor, a uniform water distribution device, a constant temperature control system and a pH control system, wherein the constant temperature control system comprises a temperature heating device, a temperature controller and a temperature probe which is arranged in the culture reactor, and the temperature probe and the temperature heating device are connected with the temperature controller; the pH control system comprises a pH controller and a pH probe which is arranged in the culture reactor; the bottom of the culture reactor is provided with an even water distribution device to realize even water distribution on the water passing section; the middle lower part of the culture reactor is a sludge bed reaction area, so that anaerobic ammonia oxidizing bacteria are fully contacted with a reaction substrate; and a three-phase separator is arranged above the sludge bed reaction zone, the floating sludge is intercepted and sinks through the three-phase separator, nitrogen generated in the culture reactor is discharged through a top gas outlet, and supernatant fluid after mud-water separation enters a water outlet pipe through an overflow weir to be discharged.
2. The device for rapidly enriching and culturing the anaerobic ammonium oxidation bacteria according to claim 1, wherein the pH controller is connected with the pH regulator medicine barrel through a medicine inlet pump.
3. The device for rapidly enriching and culturing the anaerobic ammonium oxidation bacteria according to claim 1, wherein the inlet of the culture reactor is connected with the nutrient solution dispensing barrel through a water inlet pump, and an adjusting pipeline is connected between the water inlet pump and the culture reactor and is connected with the drug inlet pump.
4. The apparatus of claim 3, wherein the nutrient solution in the nutrient solution dispensing tank is artificially synthesized sewage, and the components of the sewage comprise nitrogen source, buffer and trace elements.
5. The device for rapidly enriching and culturing the anammox according to claim 1, wherein the uniform water distribution device is a perforated flower plate, and a water inlet pipe connected with the perforated flower plate is provided with a flow controller.
6. The device for rapidly enriching and culturing the anammox according to claim 1, wherein the temperature heating device adopts a heat tracing band, the heat tracing band is uniformly arranged on the outer wall of the culture reactor, and heat insulation cotton is laid on the outer layer of the heat tracing band.
7. The device for rapidly enriching and culturing the anammox bacteria according to claim 1, wherein the culture reactor is provided with sampling monitoring ports at three different height positions.
8. The device for rapidly enriching and culturing the anammox bacteria according to claim 1, wherein a settling zone is arranged between the outer wall of the three-phase separator and the inner wall of the culture reactor, and an air chamber is arranged above the settling zone.
9. The device for rapidly enriching and culturing the anammox bacteria according to claim 1, wherein a check valve is arranged in front of an air outlet at the top of the culture reactor.
10. The device for rapidly enriching and culturing the anaerobic ammonia oxidizing bacteria according to claim 1, wherein the culture reactor adopts a cylindrical upflow anaerobic sludge blanket.
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| CN202123428406.4U CN216712108U (en) | 2021-12-31 | 2021-12-31 | Device for rapidly enriching and culturing anaerobic ammonium oxidation bacteria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115010252A (en) * | 2022-06-21 | 2022-09-06 | 深圳市深水水务咨询有限公司 | Enrichment culture device for anaerobic ammonium oxidation bacteria |
| CN116218640A (en) * | 2023-02-28 | 2023-06-06 | 乐山师范学院 | Device for domesticating concentrated sludge into Feamox flora |
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2021
- 2021-12-31 CN CN202123428406.4U patent/CN216712108U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115010252A (en) * | 2022-06-21 | 2022-09-06 | 深圳市深水水务咨询有限公司 | Enrichment culture device for anaerobic ammonium oxidation bacteria |
| CN116218640A (en) * | 2023-02-28 | 2023-06-06 | 乐山师范学院 | Device for domesticating concentrated sludge into Feamox flora |
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