CN115010252A - Enrichment culture device for anaerobic ammonium oxidation bacteria - Google Patents
Enrichment culture device for anaerobic ammonium oxidation bacteria Download PDFInfo
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- CN115010252A CN115010252A CN202210702098.XA CN202210702098A CN115010252A CN 115010252 A CN115010252 A CN 115010252A CN 202210702098 A CN202210702098 A CN 202210702098A CN 115010252 A CN115010252 A CN 115010252A
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- main body
- reactor main
- culture device
- enrichment culture
- reactor
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- 241000894006 Bacteria Species 0.000 title abstract description 19
- 230000003647 oxidation Effects 0.000 title abstract description 15
- 238000007254 oxidation reaction Methods 0.000 title abstract description 15
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 title abstract description 11
- 239000000945 filler Substances 0.000 claims abstract description 29
- 238000006243 chemical reaction Methods 0.000 claims abstract description 20
- 238000005191 phase separation Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 241001453382 Nitrosomonadales Species 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 6
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 239000010802 sludge Substances 0.000 description 12
- 239000007789 gas Substances 0.000 description 10
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000010865 sewage Substances 0.000 description 7
- 229910021529 ammonia Inorganic materials 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 239000004677 Nylon Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biological Treatment Of Waste Water (AREA)
Abstract
The invention relates to an enrichment culture device of anaerobic ammonium oxidation bacteria, which comprises a reactor main body, wherein the reactor main body is provided with a reaction zone and a three-phase separation zone from bottom to top; the reaction zone is internally provided with a filler support and a circulation impeller, the filler support comprises a fixed support at the middle part and a detachable support arranged around the fixed support, and the circulation impeller is fixed below the fixed support and is provided with a guide cylinder arranged from top to bottom. The enrichment culture device provided by the invention adopts the detachable filler support, can be taken out from the reactor main body after film hanging is finished, so that the rapid transfer of the filler is realized, and the enrichment culture device is particularly suitable for the transfer of the filler in a device with a deeper height-diameter, and realizes three-dimensional circulation in the reactor main body by adopting a form of combining a circulation impeller and a guide cylinder, thereby reducing the area of a dead zone, enhancing the mass transfer effect in the reactor and promoting the rapid growth of strains.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to an enrichment culture device for anaerobic ammonium oxidation bacteria.
Background
The anaerobic ammonia oxidation sewage treatment process is a novel sewage treatment process which can replace the traditional nitrification and denitrification process in the future due to the advantages of low energy consumption operation cost, small residual sludge amount, small process floor area and the like.
The rapid start of the anaerobic ammoxidation reactor is mainly influenced by the inoculated sludge, the regulation and control of environmental factors, the selection of fillers and carriers and the like. Theoretically, inoculation of anammox activated sludge would significantly accelerate the start-up process of anammox bacteria. However, from practical application, the anaerobic ammonium oxidation bacteria have long doubling time and slow growth rate, and less inoculation sludge can be used for starting, so that the anaerobic ammonium oxidation bacteria become a main obstacle for engineering application. Therefore, how to rapidly optimize the enrichment process of the anammox bacteria by using a trace amount of functional bacteria sources is an urgent problem to be solved in the current engineering application.
Based on the characteristics of easy aggregation and easy attachment of anammox bacteria, the method is a common mode for rapidly enriching the anammox bacteria by adding the filler into the reactor and utilizing the advantages of a microorganism immobilization technology to ensure that the bacteria are attached to the filler to grow so as to reduce strain loss. Wherein, the upflow anaerobic sludge blanket becomes an excellent anaerobic ammonia oxidizing bacteria attached growth type enrichment culture device due to the advantages of small occupied area, continuous operation, high treatment efficiency and the like. However, the anaerobic ammonium oxidation bacteria have excellent settling property, are easy to accumulate at the bottom of the device, can cause that substrates cannot be fully contacted with microorganisms, have low mass transfer efficiency and low film forming efficiency, and influence the growth speed of strains; on the other hand, the conventional upflow anaerobic sludge blanket device has higher height-diameter ratio, and the traditional filler fixing mode is adopted, so that the fillers are difficult to take out after the membrane is hung, and the transfer culture of strains cannot be realized.
Therefore, the prior art has yet to be developed.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide an enrichment culture device for anaerobic ammonium oxidation bacteria, which aims to realize rapid transfer of a filler and realize three-dimensional circulation inside a reactor main body.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides an enrichment culture device for anaerobic ammonia oxidation bacteria, which comprises a reactor main body, wherein the reactor main body is provided with a reaction zone and a three-phase separation zone from bottom to top, the bottom of the reactor main body is provided with a water inlet communicated with the reaction zone, and the upper end of the reactor main body is provided with a gas outlet and a water outlet communicated with the three-phase separation zone;
the reactor is characterized in that a filler support and a circulation impeller are arranged in the reaction area, the filler support comprises a fixed support in the middle and detachable supports arranged around the fixed support, and the circulation impeller is fixed below the fixed support and is provided with a guide cylinder arranged from top to bottom.
Further, reactor main part upper end is provided with the intercommunication the last backward flow mouth of delivery port, reactor main part lower extreme is provided with the intercommunication the lower backward flow mouth in reaction zone, go up the backward flow mouth with through the pipe connection between the backward flow mouth down, and be provided with the backwash pump.
Furthermore, a plurality of fixing buckles are arranged in the peripheral wall of the reactor main body, the detachable support is placed on the fixing buckles, the bottom of the fixing support is fixed at the bottom of the reactor main body, and the detachable support is connected with the fixing support through a connecting line.
Furthermore, the reactor main body is provided with an observation window and an access hole at the fixed buckle position.
Furthermore, a guide plate is arranged at the upper end of the three-phase separation area, one end of the guide plate is fixed inside the reactor main body, the other end of the guide plate inclines towards the inside of the reactor main body from top to bottom, a through hole is formed between the guide plates, a gas collecting hood is arranged above the through hole of the reactor main body, and the gas outlet is arranged on the gas collecting hood.
Furthermore, the lower end of the gas-collecting hood is trumpet-shaped.
The technical scheme of the invention has the following beneficial effects:
the enrichment culture device provided by the invention adopts the detachable filler support, can be taken out from the reactor main body after film hanging is finished, so that the rapid transfer of the filler is realized, and the enrichment culture device is particularly suitable for the transfer of the filler in a device with a deep height-diameter ratio.
Drawings
FIG. 1 is a schematic view of an enrichment culture apparatus according to the invention;
FIG. 2 is a top view of the inventive packing support.
Description of reference numerals:
10-a reactor main body, 11-a water inlet, 12-a gas outlet, 13-a water outlet, 14-an upper return port, 15-a lower return port, 17-a return pump, 18-a fixed buckle, 19-a water inlet pump, 101-an observation window, 102-an inspection port, 20-a reaction zone, 21-a filler support, 211-a fixed support, 212-a detachable support, 22-a circulation impeller, 221-a guide cylinder, 30-a three-phase separation zone, 31-a guide plate and 33-a gas collecting hood.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the specific embodiments described herein are only for explaining the present invention and are not intended to limit the present invention. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.
It should be noted that all directional indicators (such as upper, lower, left, right, front and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the movement situation, etc. in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly.
In the present invention, unless expressly stated or limited otherwise, the terms "connected," "secured," and the like are to be construed broadly, and for example, "connected" may be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be interconnected within two elements or in a relationship where two elements interact with each other unless otherwise specifically limited. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature.
Referring to fig. 1 and 2, the present invention provides an enrichment culture apparatus for anammox bacteria, comprising a reactor main body 10, wherein the reactor main body 10 is provided with a reaction zone 20 and a three-phase separation zone 30 from bottom to top, the bottom of the reactor main body 10 is provided with a water inlet 11 communicated with the reaction zone 20, and a water inlet pump 19 can be disposed at the water inlet 11 to accelerate the speed of sewage entering the reaction zone 20. The upper end of the reactor main body 10 is provided with an air outlet 12 and a water outlet 13 which are communicated with the three-phase separation area 30.
And a filler support 21 and a circulation flow impeller 22 are arranged in the reaction zone 20, the filler support 21 is used for the attachment growth of anaerobic ammonium oxidation bacteria to form a biological film, the concentration of internal microorganisms is improved, and the seed sludge loss is reduced. The filler is preferably suspended ball filler, sponge is arranged in the filler, and nylon ropes can be connected in series.
Specifically, the filler holder 21 includes a fixed holder 211 in the middle and a detachable holder 212 disposed around the fixed holder 211, and the circulation impeller 22 is fixed below the fixed holder 211 and is provided with a guide cylinder 221 disposed from top to bottom. The mud-water mixed liquid enters the circular flow impeller 22 from the upper part of the guide cylinder 221 to form high-speed water flow to be sprayed and diffused from the lower part to the bottom of the reactor main body 10, and the pushing flow acts on the inside of the reactor main body 10 to circulate the water flow up and down, so that the circular flow state is realized, the sludge is always in a suspension state, the area of a dead zone is reduced, the mass transfer effect is enhanced, the contact area of a substrate and anaerobic ammonium oxidation bacteria is increased, and the biological denitrification effect and the sewage treatment efficiency are improved.
The sewage firstly enters the reaction zone 20 through the water inlet pump 19 for biological denitrification, most of ammonia nitrogen and nitrite nitrogen are degraded by anaerobic ammonia oxidizing bacteria and converted into nitrogen, then the nitrogen is separated through the three-phase separation zone 30, the generated nitrogen is collected and discharged through the air outlet 12, the sewage is discharged through the water outlet 13, and floating sludge solids are intercepted and returned to the reaction zone 20.
The reactor main body 10 is provided with the circulation flow impeller 22 to realize uniform mixing of muddy water, and the guide cylinder 221 is arranged to enable the muddy water in the reaction area 20 to be in a circulation state, so that the film forming efficiency is improved, the contact area of the muddy water surface is increased, and the sludge can keep high activity.
After the film formation is finished, the detachable support 212 can be taken out from the reactor main body 10, and the fillers after the film formation are used for starting other anaerobic ammonia oxidation devices, so that the transfer culture of strains is realized, and suitable seed sludge is provided for the large-scale application of the anaerobic ammonia oxidation technology.
Further, the upper end of the reactor main body 10 is provided with an upper return port 14 communicated with the water outlet 13, the lower end of the reactor main body 10 is provided with a lower return port 15 communicated with the reaction zone 20, the upper return port 14 is connected with the lower return port 15 through a pipeline, and a return pump 17 is arranged. According to the invention, part of the effluent is refluxed by the reflux pump 17, so that reflux power is provided to enhance the mud-water mixing effect, and meanwhile, some high-concentration wastewater inlet matrixes can be diluted, so that the inhibition effect of the high-concentration matrixes on anaerobic ammonium oxidation bacteria is relieved.
As an embodiment, a plurality of fixing buckles 18 are disposed in the peripheral wall of the reactor main body 10, the detachable bracket 212 is placed on the fixing buckles 18, the bottom of the fixing bracket 211 is fixed to the bottom of the reactor main body 10, and the fixing bracket 211 can be welded on the reactor main body 10 and can be used for fixing the guide cylinder 221. The detachable support 212 is connected with the fixed support 211 through a connecting line, and the connecting line can be connected through a nylon rope.
Specifically, the reactor body 10 is provided with an observation window 101 and an access opening 102 at the fixed buckle 18, and the detachable bracket 212 can be taken out through the observation window 101 and the access opening 102, so as to facilitate the transfer of the filler.
In this embodiment, the detachable bracket 212 is a horizontally arranged layered structure, the detachable bracket 212 may be provided with multiple layers, and the detachable bracket 212 on the same level is connected to the middle fixed bracket 211 through a connecting line (such as a nylon rope), so that the detachable bracket 212 may be directly taken out through the viewing port 101 or the access port 102, thereby achieving a rapid transfer of the filler, and being particularly suitable for a transfer of the filler in a device with a deeper height and diameter.
In this embodiment, a guide plate 31 is disposed at an upper end of the three-phase separation zone 30, one end of the guide plate 31 is fixed inside the reactor main body 10, and the other end of the guide plate 31 is inclined from top to bottom toward the inside of the reactor main body 10, so that the floating sludge solids can be trapped and then returned to the reaction zone 20.
A through hole is formed between the flow guide plates 31, a gas collecting hood 33 is arranged above the through hole of the reactor main body 10, and the gas outlet 12 is arranged on the gas collecting hood 33. Preferably, the lower end of the gas-collecting hood 33 is trumpet-shaped, so that the area for collecting the generated nitrogen can be enlarged, and more and wider gas can be collected.
The above examples of the present invention are merely examples for clearly illustrating the present invention and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. Not all embodiments are exhaustive. All obvious changes and modifications which are obvious to the technical scheme of the invention are covered by the protection scope of the invention.
Claims (6)
1. The enrichment culture device for the anaerobic ammonia oxidizing bacteria is characterized by comprising a reactor main body, wherein the reactor main body is provided with a reaction zone and a three-phase separation zone from bottom to top, the bottom of the reactor main body is provided with a water inlet communicated with the reaction zone, and the upper end of the reactor main body is provided with a gas outlet and a water outlet communicated with the three-phase separation zone;
the reactor is characterized in that a filler support and a circulation impeller are arranged in the reaction area, the filler support comprises a fixed support in the middle and detachable supports arranged around the fixed support, and the circulation impeller is fixed below the fixed support and is provided with a guide cylinder arranged from top to bottom.
2. The enrichment culture device of claim 1, wherein the upper end of the reactor main body is provided with an upper return port communicated with the water outlet, the lower end of the reactor main body is provided with a lower return port communicated with the reaction zone, the upper return port and the lower return port are connected through a pipeline, and a return pump is arranged.
3. The enrichment culture device of claim 1, wherein a plurality of fixing buckles are arranged in the peripheral wall of the reactor main body, the detachable bracket is placed on the fixing buckles, the bottom of the fixing bracket is fixed at the bottom of the reactor main body, and the detachable bracket is connected with the fixing bracket through a connecting wire.
4. The enrichment culture device of claim 3, wherein the reactor body is provided with an observation window and an access opening at the fixing buckle.
5. The enrichment culture device of claim 1, wherein the upper end of the three-phase separation zone is provided with a flow guide plate, one end of the flow guide plate is fixed inside the reactor main body, the other end of the flow guide plate is inclined towards the inside of the reactor main body from top to bottom, a through hole is formed between the flow guide plates, the reactor main body is provided with a gas collecting hood above the through hole, and the gas outlet is arranged on the gas collecting hood.
6. The enrichment culture device of claim 5, wherein the lower end of the gas-collecting hood is trumpet-shaped.
Priority Applications (1)
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CN202210702098.XA CN115010252A (en) | 2022-06-21 | 2022-06-21 | Enrichment culture device for anaerobic ammonium oxidation bacteria |
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CN202210702098.XA CN115010252A (en) | 2022-06-21 | 2022-06-21 | Enrichment culture device for anaerobic ammonium oxidation bacteria |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060180546A1 (en) * | 2005-02-15 | 2006-08-17 | William L. Stuth, Sr. | Wastewater treatment system and method |
CN108609734A (en) * | 2018-07-09 | 2018-10-02 | 江苏金博源环保科技有限公司 | Anaerobic ammonia oxidation reactor |
CN209210479U (en) * | 2018-12-10 | 2019-08-06 | 河北旭杰环境工程有限公司 | Anaerobic ammonia oxidation reactor |
WO2021104541A1 (en) * | 2019-11-27 | 2021-06-03 | 苏州科技大学 | Integrated wastewater treatment apparatus and method |
CN113860488A (en) * | 2021-10-08 | 2021-12-31 | 广东中微环保生物科技有限公司 | Method and device for culturing anaerobic ammonium oxidation bacteria particles |
CN114314835A (en) * | 2021-12-31 | 2022-04-12 | 佛山市绿能环保有限公司 | Short-cut nitrification and denitrification coupling anaerobic ammonia oxidation denitrification reactor and integrated equipment |
CN216712108U (en) * | 2021-12-31 | 2022-06-10 | 大连海川博创环保科技有限公司 | Device for rapidly enriching and culturing anaerobic ammonium oxidation bacteria |
-
2022
- 2022-06-21 CN CN202210702098.XA patent/CN115010252A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
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US20060180546A1 (en) * | 2005-02-15 | 2006-08-17 | William L. Stuth, Sr. | Wastewater treatment system and method |
CN108609734A (en) * | 2018-07-09 | 2018-10-02 | 江苏金博源环保科技有限公司 | Anaerobic ammonia oxidation reactor |
CN209210479U (en) * | 2018-12-10 | 2019-08-06 | 河北旭杰环境工程有限公司 | Anaerobic ammonia oxidation reactor |
WO2021104541A1 (en) * | 2019-11-27 | 2021-06-03 | 苏州科技大学 | Integrated wastewater treatment apparatus and method |
CN113860488A (en) * | 2021-10-08 | 2021-12-31 | 广东中微环保生物科技有限公司 | Method and device for culturing anaerobic ammonium oxidation bacteria particles |
CN114314835A (en) * | 2021-12-31 | 2022-04-12 | 佛山市绿能环保有限公司 | Short-cut nitrification and denitrification coupling anaerobic ammonia oxidation denitrification reactor and integrated equipment |
CN216712108U (en) * | 2021-12-31 | 2022-06-10 | 大连海川博创环保科技有限公司 | Device for rapidly enriching and culturing anaerobic ammonium oxidation bacteria |
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高大文等: "《环境工程学》", 31 July 2017, 哈尔滨工业大学出版社 * |
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Application publication date: 20220906 |