CN201952429U - Cell exposure-based contamination control device for cigarette smoke - Google Patents
Cell exposure-based contamination control device for cigarette smoke Download PDFInfo
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- CN201952429U CN201952429U CN 201020656082 CN201020656082U CN201952429U CN 201952429 U CN201952429 U CN 201952429U CN 201020656082 CN201020656082 CN 201020656082 CN 201020656082 U CN201020656082 U CN 201020656082U CN 201952429 U CN201952429 U CN 201952429U
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- smoke
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- control device
- cellular exposure
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Abstract
The utility model provides a cell exposure-based contamination control device for cigarette smoke, which is characterized in that one air inlet end of a main air pipe is connected with a cigarette smoke generator, while the other end of the main air pipe is connected with a purification treatment device via an exhaust pipe which is provided with a flow controller. An air inlet pipe is connected with a cell exposure cavity via a diffusion port, and an air outlet is connected with a smoke collecting cavity via a collecting port. An isolation cover is arranged between the smoke collecting cavity and the cell exposure cavity, and a cell diaphragm plate is arranged between the cell exposure cavity and a cell culture fluid cavity and a warm bath cavity is arranged outside the cell diaphragm plate. A culture fluid supplying device is connected with the cell culture fluid cavity. The cell exposure-based contamination control device for cigarette smoke has the beneficial effects: experimental animals are replaced with in-vitro cultures in a contamination experiment on cigarette smoke to optimize experimental methods, the contamination experiment is directly carried out on cultures, such as target organ cells, and the like so as to conveniently control the full process of the experiment, thus the accurate experimental results are obtained. The cell exposure-based contamination control device for cigarette smoke can be widely applied in the cell exposure-based contamination experiments for such fields as medical science, public health and environmental sciences, molecular biology, cytology, toxicology and the like.
Description
Technical field
The utility model relates to a kind of cellular exposure smoke from cigarette inhalation control device.
Background technology
In the scientific domains such as present stage biology, environmentalism, diseases prevention and control, medical science, military science, toxicology, suck the experiment of smoke from cigarette toxic exposure and also rest on animal experiment stage.This exists that the laboratory animal quantity that needs is many, experiment condition is harsh, animal individual difference causes greatly that experiment effect is not good, the cost height of testing and the requirement of animal welfare and protection aspect etc. are unfavorable for factor seriously restricting going deep into of this type of research.And the appearance of cell toxicology research and swift and violent a kind of can accurately control and the direct smoke from cigarette exposure experimental device of effect of development exigence.
The utility model content
For addressing the above problem, the utility model provides a kind of cellular exposure smoke from cigarette inhalation control device, gets rid of the interference of other factors in the experiment, and target organ is directly carried out the smoke from cigarette exposure experiment, and can control the experiment whole process.
For achieving the above object, the technical solution of the utility model provides a kind of cellular exposure smoke from cigarette inhalation control device, comprise that main tracheae air inlet one end is connected with the smoke from cigarette generating unit, the other end links to each other with purifying processing device by the vapor pipe that has flow director.Inlet pipe links to each other with the cellular exposure chamber by the diffusion gas port, and venting port links to each other with smoke collecting chamber by collecting gas port.The lid of isolation is arranged between smoke collecting chamber and the cellular exposure chamber, between cellular exposure chamber and the cell cultures sap cavity cell lamina membranacea is arranged, this part has temperature to bathe the chamber outward, connects temperature and bathes control device; The nutrient solution feeding mechanism links to each other with the cell cultures sap cavity.
There is the isolation lid that evenly is covered with aperture in described cellular exposure chamber, it on it smoke collecting chamber, be placed with the cell lamina membranacea between itself and the cell cultures sap cavity, cell lamina membranacea upper surface is exposed in the cellular exposure chamber fully, lower surface soaks in the cell cultures sap cavity, and this four part of smoke collecting chamber, cellular exposure chamber, cell cultures sap cavity and cell lamina membranacea is all bathed chamber institute parcel fully by temperature.
Between main tracheae and the smoke from cigarette generating unit, on the inlet air source, inlet pipe, vapor pipe flow director is housed respectively.
Described inlet pipe bottom is for spreading gas port in the cellular exposure chamber, and the vapor pipe bottom is for collecting gas port in smoke collecting chamber.
Described temperature is bathed between control device and the temperature bath chamber to be connected by conduit and is formed the temperature control loop.
Described cell cultures sap cavity and be equipped with between the nutrient solution feeding mechanism of nutrient solution flow director the current stabilization pump is housed, the other end is equipped with cell culture fluid water conservancy diversion delivery pipe.
Described smoke from cigarette generating unit is inner for the cigarette rotating disk by the equidistant cigarette pickup groove of driven by motor is arranged, and the cigarette rotating disk has telescopic electronic cigarette lighter down for the cigarette ash pallet on smoke from cigarette generating unit sidewall; Motor and electronic cigarette lighter are subjected to computer control simultaneously.
Described smoke from cigarette generating unit links to each other with inlet air source, and inlet air source is given smoke from cigarette generating unit supply air inlet, and the drive smoke from cigarette enters the cellular exposure chamber.
Its beneficial effect is to substitute laboratory animal with the vitro culture thing in the smoke from cigarette exposure experiment, has optimized experimental technique, and cultures such as target organ cell are directly carried out exposure experiment, and the whole process of control experiment easily obtains accurate experimental result.Can be widely used in the cellular exposure smoke from cigarette exposure experiment in each field such as medical science, public hygienic environment, molecular biology, cytology, toxicology.
Description of drawings
Accompanying drawing is the synoptic diagram of the utility model structure.
Among the figure:
1, purifying processing device 2, vapor pipe 3, collection gas port
4, diffusion gas port 5, conduit 6, temperature are bathed control device
7, nutrient solution feeding mechanism 8, nutrient solution flow director
9, current stabilization pump 10, temperature are bathed chamber 11, cell cultures sap cavity
12, cell culture fluid water conservancy diversion delivery pipe 13, cell lamina membranacea
14, cellular exposure chamber 15, smoke collecting chamber 16, isolation lid
17, inlet pipe 18, main tracheae 19, flow director
20, smoke from cigarette generating unit 21, cigarette rotating disk 22, cigarette pickup groove
23, electronic cigarette lighter 24, computer 25, motor
26, cigarette ash pallet 27, inlet air source
Embodiment
In conjunction with the accompanying drawings the utility model is illustrated.
As shown in drawings, main tracheae 18 air inlets one end is connected with smoke from cigarette generating unit 20 and inlet air source 27, and the end of giving vent to anger links to each other with purifying processing device 1 by flow director 19.Purifying processing device 1 can carry out thorough purifying treatment with experiment waste gas and reach safety dumping.The isolation lid 16 that evenly is covered with aperture is arranged at 14 tops, cellular exposure chamber, its top is smoke collecting chamber 15, be placed with cell lamina membranacea 13 between cellular exposure chamber 14 and the cell cultures sap cavity 11, cell lamina membranacea 13 upper surfaces are exposed in the cellular exposure chamber 14 fully, and lower surface soaks in cell cultures sap cavity 11.The nutrient solution feeding mechanism 7 that nutrient solution flow director 8 is housed on it links to each other with cell cultures sap cavity 11, and current stabilization pump 9 is housed between the two, and the other end is equipped with cell culture fluid water conservancy diversion delivery pipe 12, guarantees that the required nutrition of cell growth is constantly supplied.
And smoke collecting chamber 15, cellular exposure chamber 14, cell cultures sap cavity 11 and cell lamina membranacea 13 these four parts are all wrapped up fully by 10 in temperature bath chamber.Temperature is bathed chamber 10 and is wrapped in whole cellular exposure chamber 14, cell lamina membranacea 13 and cell cultures sap cavity 11 outsides fully, and bathes between the control device 6 with temperature and to be connected by conduit 5, and the temperature that temperature is bathed in the chamber 10 keep constant.
The cigarette rotating disk 21 of the equidistant cigarette pickup groove 22 of smoke from cigarette generating unit 20 inside rotates under the drive of motor 25, it contacts with electronic cigarette lighter 23 on smoke from cigarette generating unit 20 sidewalls and lights cigarette, and the length of the cigarette that electronic cigarette lighter 23 can be lighted is as required stretched; The cigarette rotating disk is a cigarette ash pallet 26 21 times, collects the cigarette ash after the burning; Motor 25 and electronic cigarette lighter 23 are subjected to computer 24 controls simultaneously, accurately locate the rotating speed and the position of cigarette rotating disk, and can adjust the stroke of electronic cigarette lighter 23 automatically.
On inlet pipe 17 on smoke from cigarette generating unit 20, inlet air source 27, the main tracheae 18 and vapor pipe 2, flow director 19 is housed respectively, can accurately controls the generating capacity of smoke from cigarette and concentration etc.There is diffusion gas port 4 inlet pipe 17 bottoms in cellular exposure chamber 14, can fully cover cell lamina membranacea 13 and make the abundant exposing cell upper surface of gaseous substance in the cellular exposure chamber 14; There is the gas port 3 of collection vapor pipe 2 bottoms in smoke collecting chamber 15, can collect fully by the discarded smoke from cigarette of isolating the aperture derivation of covering on 16.
The structure of described herein device can be applied to simultaneously a plurality of by inlet pipe 17, vapor pipe 2, smoke collecting chamber 15, have to isolate and cover the experiment module that 16 cellular exposure chamber 14, cell lamina membranacea 13, cell cultures sap cavity 11 and temperature bath chamber 10 are formed.
When using, at first open cellular exposure chamber 14, the cell lamina membranacea 13 that has experimental cell will be isolated after being placed under the aseptic condition on the cell cultures sap cavity 11, steadily putting well with cultivating cover 16 and cover completely.Regulate flow director 19 and nutrient solution flow director 8 then, open nutrient solution feeding mechanism 7 and temperature bath control device 6 and inlet air source 27.Cigarette is put into cigarette pickup groove 22, build smoke from cigarette generating unit 20, adjust computer program, light cigarette, smoke from cigarette enters main tracheae 18 and is transported to uniformly in the cellular exposure chamber 14 by diffusion gas port 4, and the upper surface that evenly spreads in experimental cell carries out the smoke from cigarette exposure experiment to experimental cell.Waste gas is collected in the purifying processing device 1 by the collection gas port 3 of vapor pipe 2 bottoms, carries out safe handling.After experiment finishes, take out the cell lamina membranacea 13 that has experimental cell and carry out the poisonous effect analysis, thereby obtain the toxicity analytical results of science.
Claims (8)
1. cellular exposure smoke from cigarette inhalation control device, it is characterized in that: comprise that main tracheae (18) air inlet one end is connected with smoke from cigarette generating unit (20), the other end links to each other with purifying processing device (1) by the vapor pipe (2) that has flow director (19), inlet pipe (17) links to each other with cellular exposure chamber (14) by diffusion gas port (4), venting port links to each other with smoke collecting chamber (15) by collecting gas port (3), the lid of isolation (16) is arranged between smoke collecting chamber (15) and cellular exposure chamber (14), between cellular exposure chamber (14) and the cell cultures sap cavity (11) cell lamina membranacea (13) is arranged, this part has temperature to bathe chamber (10) connection temperature bath control device (6) outward, and nutrient solution feeding mechanism (7) links to each other with cell cultures sap cavity (11).
2. cellular exposure smoke from cigarette inhalation control device according to claim 1, it is characterized in that: there is the isolation lid (16) that evenly is covered with aperture in described cellular exposure chamber (14), on it smoke collecting chamber (15), be placed with cell lamina membranacea (13) between itself and the cell cultures sap cavity (11), cell lamina membranacea (13) upper surface is exposed in the cellular exposure chamber (14) fully, lower surface soaks in cell cultures sap cavity (11), and smoke collecting chamber (15), cellular exposure chamber (14), cell cultures sap cavity (11) and this four part of cell lamina membranacea (13) are all bathed chamber (10) institute parcel fully by temperature.
3. cellular exposure smoke from cigarette inhalation control device according to claim 1 is characterized in that: between main tracheae (18) and the smoke from cigarette generating unit (20), on the inlet air source (27), inlet pipe (17), vapor pipe (2) flow director (19) is housed respectively.
4. cellular exposure smoke from cigarette inhalation control device according to claim 1, it is characterized in that: described inlet pipe (17) bottom is for spreading gas port (4) in cellular exposure chamber (14), and vapor pipe (2) bottom is for collecting gas port (3) in smoke collecting chamber (15).
5. cellular exposure smoke from cigarette inhalation control device according to claim 1 is characterized in that: described temperature is bathed between control device (6) and the temperature bath chamber (10) to be connected by conduit (5) and is formed the temperature control loop.
6. cellular exposure smoke from cigarette inhalation control device according to claim 1, it is characterized in that: described cell cultures sap cavity (11) and be equipped with between the nutrient solution feeding mechanism (7) of nutrient solution flow director (8) current stabilization pump (9) is housed, the other end is equipped with cell culture fluid water conservancy diversion delivery pipe (12).
7. cellular exposure smoke from cigarette inhalation control device according to claim 1, it is characterized in that: described smoke from cigarette generating unit (20) is inner for the cigarette rotating disk (21) of the equidistant cigarette pickup groove (22) that is driven by motor (25) is arranged, cigarette rotating disk (21) for cigarette ash pallet (26), has telescopic electronic cigarette lighter (23) down on smoke from cigarette generating unit (20) sidewall; Motor (25) and electronic cigarette lighter (23) are subjected to computer (24) control simultaneously.
8. cellular exposure smoke from cigarette inhalation control device according to claim 1, it is characterized in that: described smoke from cigarette generating unit (20) links to each other with inlet air source (27), inlet air source (27) is given smoke from cigarette generating unit (20) supply air inlet, and the drive smoke from cigarette enters cellular exposure chamber (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020656082 CN201952429U (en) | 2010-12-13 | 2010-12-13 | Cell exposure-based contamination control device for cigarette smoke |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020656082 CN201952429U (en) | 2010-12-13 | 2010-12-13 | Cell exposure-based contamination control device for cigarette smoke |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201952429U true CN201952429U (en) | 2011-08-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020656082 Expired - Fee Related CN201952429U (en) | 2010-12-13 | 2010-12-13 | Cell exposure-based contamination control device for cigarette smoke |
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| CN (1) | CN201952429U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154433A (en) * | 2010-12-13 | 2011-08-17 | 天津开发区合普工贸有限公司 | Cell-exposed contamination control device for cigarette smoke |
-
2010
- 2010-12-13 CN CN 201020656082 patent/CN201952429U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154433A (en) * | 2010-12-13 | 2011-08-17 | 天津开发区合普工贸有限公司 | Cell-exposed contamination control device for cigarette smoke |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110831 Termination date: 20161213 |