CN215328152U

CN215328152U - Cell culture device

Info

Publication number: CN215328152U
Application number: CN202121089277.8U
Authority: CN
Inventors: 姚鲁帅; 宋姗姗; 王燚
Original assignee: Wuxi Zenzer Accurate Medical Laboratories Co ltd
Current assignee: Wuxi Zenzer Accurate Medical Laboratories Co ltd
Priority date: 2021-05-20
Filing date: 2021-05-20
Publication date: 2021-12-28
Anticipated expiration: 2031-05-20

Abstract

The utility model discloses a cell culture device, belonging to the related technical field of medical equipment, the technical scheme is characterized in that the device comprises a placing box, a plurality of uniformly distributed placing cavities are arranged on the front end surface of the placing box, a culture shelf is arranged in the middle of each placing cavity, a plurality of uniformly distributed culture containers are arranged in the middle of the upper part of the culture shelf, an oxygen box, a controller and a ventilator are sequentially arranged on the upper end surface of the placing box from left to right, one side of the oxygen box, opposite to the ventilator, is respectively communicated with an oxygen conveying tank and an exhaust pipe, the left side and the right side of each placing cavity are respectively communicated with an oxygen flow dividing pipe and an exhaust flow dividing pipe which are matched with the oxygen conveying tank and the exhaust pipe, air detection probes are arranged at the top ends of the inner side walls of the placing cavities, the oxygen box is started, oxygen can be conveniently conveyed into the placing cavities, the requirement on oxygen during cell culture is met, and the ventilator is started, the gas in the placing cavity achieves the effects of replacement and circulation, and the quality of cell culture is improved.

Description

Cell culture device

Technical Field

The utility model relates to the technical field related to medical equipment, in particular to a cell culture device.

Background

Cells, which are the basic units of organisms in biology, were discovered by the british scientist robert hooke in 1665, and were composed mainly of nuclei and cytoplasm, with cell membranes on the surface. The cells can be divided into prokaryotic cells and eukaryotic cells, have functions of movement, nutrition, propagation and the like, and all organisms except viruses are known to be composed of the cells, but the vital activities of the viruses can be embodied only in the cells.

Need oxygen in the cell culture process and need the culture solution again, simultaneously, the circulation of air, and current cell culture device simple structure has reduced cell culture's quality.

SUMMERY OF THE UTILITY MODEL

The present invention has been made in view of the above problems, and provides a cell culture apparatus to solve the above problems.

The utility model is realized in such a way, the cell culture device comprises a placing box, a plurality of evenly distributed placing cavities are arranged on the front end surface of the placing box, a culture shelf is arranged in the middle of each placing cavity, a plurality of evenly distributed culture containers are arranged in the middle of the upper part of the culture shelf, an oxygen box, a controller and a ventilator are sequentially arranged on the upper end surface of the placing box from left to right, a conveying oxygen tank and an exhaust tube are respectively communicated with one side of the oxygen box opposite to the ventilator, an oxygen shunt tube and an exhaust shunt tube which are matched with the conveying oxygen tank and the exhaust tube are respectively communicated with the left side and the right side of each placing cavity, air detection probes are respectively arranged at the top ends of the inner side walls of the placing cavities, ultraviolet sterilizing lamps are respectively arranged at the left side and the right side of each air detection probe, electric control valves which are matched with the oxygen shunt tube and the exhaust shunt tube are respectively arranged at the left side and the right side of the placing box, cell culture grooves are formed in the middle of the upper end faces of the culture containers, culture covers are arranged above the cell culture grooves, the left side and the right side of each culture cover are connected with the culture containers in a sliding mode, and a plurality of through holes which are uniformly distributed penetrate through the upper end faces of the culture covers;

cultivate the middle part of frame and be provided with and place and separate the frame, place the mid-mounting that separates the frame up end have a plurality ofly and cultivate container assorted protective sheath, cultivate the preceding terminal surface of frame and install temperature operating panel and handle from a left side to the right side in proper order.

In order to conveniently and stably place the culture rack in the placing cavity, the cell culture device is preferably used as the cell culture device provided by the utility model, the left side and the right side of the side walls of the placing cavities are respectively provided with a clamping groove, and the left side and the right side of the culture rack are respectively provided with a clamping piece matched with the clamping grooves.

In order to facilitate the culture container to be taken out from the upper part of the culture shelf by using the holding rod, the cell culture device is preferably used as the cell culture device of the utility model, the outer side wall of the culture container is fixedly connected with a limiting sleeve, the holding rod is arranged above the limiting sleeve, and the middle part in front of the upper end surface of the culture cover is fixedly connected with a push block.

In order to prevent bacteria and dust from entering the placing box, it is preferable that a box door is movably connected to the left side of the front end surface of the placing box.

In order to facilitate the movement of the position of the placing box, it is preferable that a plurality of universal wheels are uniformly distributed and fixedly connected to the lower end surface of the placing box.

Compared with the prior art, the utility model has the beneficial effects that:

1. the cell culture device is characterized in that a plurality of uniformly distributed placing cavities are formed in the front end face of a placing box, cultured cells of different types are conveniently placed in the placing cavities respectively, the placing cavities are connected in a clamping mode to install a culture frame, a plurality of culture containers are further convenient to place above the culture frame, an oxygen box and an air interchanger are installed on the upper end face of the placing box, the oxygen box is started to conveniently convey oxygen to the placing cavities, the requirement for oxygen during cell culture is met, the air interchanger is started to enable gas in the placing cavities to achieve the effect of replacement and circulation, the quality of cell culture is improved, an air detection probe and an ultraviolet germicidal lamp are installed at the top end of the side wall in the placing cavities, the ultraviolet germicidal lamp is convenient to sterilize the placing cavities regularly, the air detection probe is convenient to monitor the air content in the placing cavities in real time, and an electric control valve above an oxygen shunt tube and an air exhaust shunt tube is convenient to open in time, the air in the placing cavity is adjusted, the culture cover is arranged above the cell culture tank, when the culture container is taken out of the placing box for inspection, the culture cover reduces the entry of bacteria, and the through hole is formed above the culture cover, so that the oxygen can conveniently enter, meanwhile, nutrient solution can be conveniently dripped into the cell culture tank, and the service performance of the cell culture device is enhanced;

2. this kind of cell culture device, through the mid-mounting placing the shelf up end have a plurality ofly with cultivate container assorted protective sheath, and then conveniently place a plurality of culture containers above cultivateing the shelf, at the preceding terminal surface installation temperature operating panel who cultivates the shelf, the inside of cultivateing the shelf is provided with the warmer, through the temperature that temperature operating panel conveniently adjusted cultivation shelf, guarantee that the cell cultivates under suitable temperature, the handle of setting is conveniently got and is put cultivation shelf, improves cell culture's quality and efficiency.

Drawings

FIG. 1 is a view showing the overall configuration of a cell culture apparatus according to the present invention;

FIG. 2 is a view showing the internal structure of the placing box of the present invention;

FIG. 3 is a schematic view of the structure of a culture shelf and a culture vessel according to the present invention;

FIG. 4 is a schematic diagram of a culture vessel of the present invention.

In the figure, 1, a placing box; 101. a box door; 102. a universal wheel; 103. a placement chamber; 104. an air detection probe; 105. an ultraviolet germicidal lamp; 106. a clamping groove; 2. an oxygen tank; 201. a delivery oxygen tank; 202. an oxygen shunt tube; 3. a ventilator; 301. an air exhaust pipe; 302. an air exhaust shunt pipe; 4. a controller; 401. an electrically controlled valve; 5. a culture shelf; 501. a clamping piece; 502. a handle; 503. a temperature operating panel; 504. placing a separation frame; 505. a protective sleeve; 6. a culture vessel; 601. a holding rod; 602. a limiting sleeve; 603. a cell culture tank; 604. a culture cover; 605. and a through hole.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.

In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

Referring to fig. 1-4, the present invention provides a technical solution: a cell culture device comprises a placing box 1, a plurality of uniformly distributed placing cavities 103 are formed in the front end face of the placing box 1, a culture frame 5 is arranged in the middle of each placing cavity 103, a plurality of uniformly distributed culture containers 6 are arranged in the middle of the upper portion of the culture frame 5, an oxygen box 2, a controller 4 and a ventilator 3 are sequentially arranged on the upper end face of the placing box 1 from left to right, a conveying oxygen tank 201 and an exhaust tube 301 are respectively communicated with one side of each oxygen box 2 opposite to the ventilator 3, oxygen shunt tubes 202 and exhaust shunt tubes 302 matched with the conveying oxygen tank 201 and the exhaust tube 301 are respectively communicated with the left side and the right side of the placing cavity 103, air detecting probes 104 are respectively arranged at the top ends of the inner side walls of the placing cavities 103, ultraviolet sterilizing lamps 105 are respectively arranged on the left side and the right side of each air detecting probe 104, electric control valves 401 matched with the oxygen shunt tubes 202 and the exhaust shunt tubes 302 are respectively arranged on the left side and the right side of the placing box 1, the middle parts of the upper end surfaces of the plurality of culture containers 6 are provided with cell culture grooves 603, culture covers 604 are arranged above the cell culture grooves 603, the left side and the right side of each culture cover 604 are connected with the culture containers 6 in a sliding manner, and the upper end surfaces of the culture covers 604 are provided with a plurality of through holes 605 which are uniformly distributed in a penetrating manner;

the middle part of the culture shelf 5 is provided with a placing separation shelf 504, the middle part of the upper end surface of the placing separation shelf 504 is provided with a plurality of protective sleeves 505 matched with the culture containers 6, and the front end surface of the culture shelf 5 is sequentially provided with a temperature operation panel 503 and a handle 502 from left to right.

In this embodiment: a plurality of evenly distributed placing cavities 103 are formed in the front end face of a placing box 1, and then cultured cells of different types are respectively and conveniently placed in the placing cavities 103, a culture frame 5 is installed in the placing cavities 103 in a clamping manner, a plurality of culture containers 6 are further convenient to place above the culture frame 5, an oxygen box 2 and a ventilator 3 are installed on the upper end face of the placing box 1, the oxygen box 2 is started to conveniently convey oxygen into the placing cavities 103, the requirement of the cells on oxygen during sample culture is met, the ventilator 3 is started, so that the gas in the placing cavities 103 achieves the effects of replacement and circulation, the quality of cell culture is improved, an air detecting probe 104 and an ultraviolet sterilizing lamp 105 are installed at the top end of the inner side wall of the placing cavities 103, the ultraviolet sterilizing lamp 105 is convenient for regularly sterilizing the placing cavities 103, the air detecting probe 104 is convenient for monitoring the air content in the placing cavities 103 in real time, and is convenient for opening an electric control valve 401 above an oxygen shunt pipe 202 and an air exhaust shunt pipe 302 in time, the air in the placing cavity 103 is adjusted, the culture cover 604 is arranged above the cell culture tank 603, when the culture container 6 is taken out of the placing box 1 for inspection, the culture cover 604 reduces the entering of bacteria, and the through hole 605 is arranged above the culture cover 604, so that the oxygen can enter conveniently, meanwhile, nutrient solution can be dripped into the cell culture tank 603 conveniently, and the service performance of the cell culture device is enhanced;

through placing the mid-mounting that separates frame 504 up end have a plurality ofly with cultivate 6 assorted protective sheaths 505 of container, and then conveniently place a plurality of culture containers 6 above cultivateing frame 5, install temperature operating panel 503 at the preceding terminal surface of cultivateing frame 5, the inside of cultivateing frame 5 is provided with the warmer, conveniently adjust the temperature of cultivateing frame 5 through temperature operating panel 503, guarantee that the cell is cultivateed under suitable temperature, the handle 502 of setting conveniently gets puts and cultivates frame 5, improve cell culture's quality and efficiency.

As a technical optimization scheme of the utility model, the left side and the right side of the inner side walls of the plurality of placing cavities 103 are both provided with the clamping grooves 106, and the left side and the right side of the culture rack 5 are both provided with the clamping pieces 501 matched with the clamping grooves 106.

In this embodiment: through all being provided with in the left and right sides of cultivateing frame 5 with joint groove 106 assorted joint spare 501, further make things convenient for stable placing of cultivateing frame 5 to place in the chamber 103.

As a technical optimization scheme of the utility model, the outer side wall of the culture container 6 is fixedly connected with a limiting sleeve 602, a holding rod 601 is arranged above the limiting sleeve 602, and the middle part in front of the upper end surface of the culture cover 604 is fixedly connected with a push block.

In this embodiment: by arranging the holding rod 601 above the limiting sleeve 602, the culture container 6 can be conveniently taken out from the upper part of the culture shelf 5 by using the holding rod 601, and the culture cover 604 can be conveniently opened by the push block.

As a technical optimization scheme of the utility model, the left side of the front end surface of the placing box 1 is movably connected with a box door 101.

In this embodiment: through the left side swing joint at placing case 1 front end face have chamber door 101, chamber door 101 plays sealed effect, prevents that bacterium and dust from entering into and placing in the case 1.

As a technical optimization scheme of the present invention, the lower end surface of the placing box 1 is fixedly connected with a plurality of uniformly distributed universal wheels 102.

In this embodiment: the lower end face of the placing box 1 is fixedly connected with a plurality of universal wheels 102 which are uniformly distributed, so that the position of the placing box 1 is further convenient to move.

The working principle is as follows: firstly, a plurality of evenly distributed placing cavities 103 are arranged on the front end face of a placing box 1, and then cultured cells of different types are respectively and conveniently placed in the placing cavities 103, a culture frame 5 is clamped and installed in the placing cavity 103, a plurality of culture containers 6 are further and conveniently placed above the culture frame 5, an oxygen box 2 and a ventilator 3 are installed on the upper end face of the placing box 1, the oxygen box 2 is started to conveniently convey oxygen into the placing cavity 103, the requirement of the cells on the oxygen during sample culture is met, the ventilator 3 is started, so that the gas in the placing cavity 103 achieves the effects of replacement and circulation, the quality of cell culture is improved, an air detecting probe 104 and an ultraviolet sterilizing lamp 105 are installed at the top end of the inner side wall of the placing cavity 103, the ultraviolet sterilizing lamp 105 is convenient for regularly sterilizing the placing cavity 103, and the air detecting probe 104 is convenient for monitoring the air content in the placing cavity 103 in real time, it in time opens the automatically controlled valve 401 of oxygen shunt tubes 202 and the shunt tubes 302 top of bleeding to be convenient for, adjust and place the air in chamber 103, top at cell culture groove 603 sets up cultivates lid 604, when cultivateing container 6 and taking to place the outside of case 1 and examining, cultivate the entering that lid 604 reduces the bacterium, set up through-hole 605 above cultivateing lid 604, not only make things convenient for the entering of oxygen, and simultaneously, be convenient for to drip the nutrient solution in cell culture groove 603, make cell culture apparatus's performance reinforcing, the inside of cultivateing frame 5 is provided with the warmer, conveniently adjust the temperature of cultivateing frame 5 through temperature operating panel 503, guarantee that the cell cultivates under the suitable temperature, the handle 502 that sets up makes things convenient for cultivateing frame 5, improve cell culture's quality and efficiency of getting and putting.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell culture device, including placing case (1), its characterized in that: the front end face of the placing box (1) is provided with a plurality of uniformly distributed placing cavities (103), the middle parts of the placing cavities (103) are provided with a culture frame (5), the middle part above the culture frame (5) is provided with a plurality of uniformly distributed culture containers (6), the upper end face of the placing box (1) is sequentially provided with an oxygen box (2), a controller (4) and a ventilator (3) from left to right, one side of the oxygen box (2) opposite to the ventilator (3) is respectively communicated with an oxygen conveying tank (201) and an air exhaust pipe (301), the left side and the right side of the placing cavity (103) are respectively communicated with an oxygen shunt pipe (202) and an air exhaust shunt pipe (302) which are matched with the oxygen conveying tank (201) and the air exhaust pipe (301), the top ends of the inner side walls of the placing cavity (103) are respectively provided with an air detection probe (104), and the left side and the right side of the air detection probe (104) are respectively provided with an ultraviolet sterilizing lamp (105), the left side and the right side of the placing box (1) are respectively provided with an electric control valve (401) matched with the oxygen shunt pipe (202) and the air exhaust shunt pipe (302), the middle parts of the upper end surfaces of the culture containers (6) are respectively provided with a cell culture tank (603), a culture cover (604) is arranged above the cell culture tanks (603), the left side and the right side of the culture cover (604) are connected with the culture containers (6) in a sliding way, and the upper end surface of the culture cover (604) is provided with a plurality of through holes (605) which are uniformly distributed in a penetrating way;

cultivate the middle part of frame (5) and be provided with and place and separate frame (504), place the mid-mounting that separates frame (504) up end have a plurality ofly with cultivate container (6) assorted protective sheath (505), cultivate the preceding terminal surface of frame (5) and install temperature operating panel (503) and handle (502) from a left side to the right side in proper order.

2. A cell culture apparatus according to claim 1, wherein: a plurality of place the left and right sides of chamber (103) inside wall and all seted up joint groove (106), cultivate the left and right sides of frame (5) and all be provided with joint groove (106) assorted joint spare (501).

3. A cell culture apparatus according to claim 1, wherein: the outer side wall of the culture container (6) is fixedly connected with a limiting sleeve (602), a holding rod (601) is arranged above the limiting sleeve (602), and a pushing block is fixedly connected with the middle part in front of the upper end face of the culture cover (604).

4. A cell culture apparatus according to claim 1, wherein: the left side of the front end face of the placing box (1) is movably connected with a box door (101).

5. A cell culture apparatus according to claim 1, wherein: the lower end face of the placing box (1) is fixedly connected with a plurality of universal wheels (102) which are uniformly distributed.