CN111979123A - Stem cell culture equipment for cell genetic engineering - Google Patents

Stem cell culture equipment for cell genetic engineering Download PDF

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Publication number
CN111979123A
CN111979123A CN202010765070.1A CN202010765070A CN111979123A CN 111979123 A CN111979123 A CN 111979123A CN 202010765070 A CN202010765070 A CN 202010765070A CN 111979123 A CN111979123 A CN 111979123A
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incubator
side wall
wall
cell culture
fixedly arranged
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周代远
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Individual
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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Abstract

The invention discloses a stem cell culture device for cell genetic engineering, which comprises: an incubator; the isolation structure is fixedly arranged in the incubator and is positioned at the central part; the throwing structure is fixedly arranged on the inner lower wall of the incubator and is positioned on the central line of the left end; the invention relates to the technical field of cell culture, in particular to a bearing control structure which is fixedly arranged in an incubator and positioned at the right end, wherein cell sap is placed on a clamping claw, and a test tube which is initially put in is isolated and sterilized through an isolation structure and then can be placed on a bracket; the bearing control structure can be connected with external equipment to control the environment in the simulated incubator so as to ensure the normal culture and division of the cell sap; the device integrally realizes sterilization, putting and environment simulation for cell culture; not only can avoid external factors to influence the experiment, simultaneously the cost is lower, the control operation of being convenient for.

Description

Stem cell culture equipment for cell genetic engineering
Technical Field
The invention relates to the technical field of cell culture, in particular to stem cell culture equipment for cell genetic engineering.
Background
The stem cell is a pluripotent cell with self-replication capacity, and can be differentiated into a plurality of functional cells under certain conditions; dividing the stem cells into embryonic stem cells and adult stem cells according to the development stage of the stem cells;
in genetic engineering, artificial culture is often carried out, and a simulated environment is needed to ensure that cells are normally generated by division without external interference; therefore, culture equipment is often needed, and in the use of the existing culture equipment, the cell sap test tube is inevitably contacted with air, impurities and the like in the process of being placed into the cell culture equipment, so that the influence on the later culture is caused, and therefore, the stem cell culture equipment for cell genetic engineering is designed.
Disclosure of Invention
The invention aims to provide stem cell culture equipment for cell genetic engineering, which aims to solve the problem of preventing pollution caused in the test tube putting process.
In order to achieve the purpose, the invention provides the following technical scheme: a stem cell culture apparatus for cell genetic engineering, comprising:
an incubator;
the isolation structure is fixedly arranged in the incubator and is positioned at the central part;
the throwing structure is fixedly arranged on the inner lower wall of the incubator and is positioned on the central line of the left end;
the bearing control structure is fixedly arranged in the incubator and is positioned at the right end;
further comprising: the incubator is of a rectangular box body structure without a left side wall, and one end of the door body is movably arranged on the rear side wall edge of the left end of the incubator;
the isolation structure includes: the sterilizing lamp comprises a partition plate, a sealing strip, a baffle plate, a first electric push rod and a plurality of sterilizing lamps with the same structure;
the upper end and the lower end of the baffle plate are respectively fixedly welded on the upper side wall and the lower side wall in the incubator and are positioned at the central part, a throwing opening is formed in the central part of the top end of the baffle plate, the sealing strip is fixedly arranged on the left side wall of the baffle plate and is positioned at the throwing opening, one end of the baffle plate is movably arranged on the left side wall of the baffle plate and is positioned on a central line, the other end of the baffle plate is arranged on the throwing opening in a knocking mode, one end of the first electric push rod is movably connected to the left side wall of the baffle plate and is positioned at the central part, the other end of the first electric push rod is movably arranged on the central line of the lower wall in the incubator, and the sterilization lamps are respectively fixedly.
Preferably, the releasing structure includes: the device comprises a main body cylinder, a second electric push rod, a bearing cylinder, a pair of limiting columns with the same structure, a motor, a mounting frame and a throwing component;
the main body cylinder is of a cylindrical structure without an upper wall, the left side wall and the right side wall of the main body cylinder are provided with clamping grooves which are identical in structure and symmetrical with each other in an opening and closing manner, one of the second electric push rods is fixedly embedded in the main body cylinder, the bearing cylinder is of a cylindrical structure without an upper wall, the bearing cylinder is fixedly arranged on the telescopic end of the second electric push rod, the lower wall of the bearing cylinder is attached to the upper wall edge of the main body cylinder, the left side wall and the right side wall of the bearing cylinder are provided with engaging grooves which are identical in structure in an opening and closing manner, one end of each limiting column is fixedly welded to the lower wall of the bearing cylinder and is respectively positioned at the left end and the right end in a symmetrical manner, the other end of each limiting column is movably inserted in the clamping groove of the main body cylinder, the motor is movably embedded in the bearing cylinder, the left side wall and the right side wall of the bearing cylinder are fixedly provided with, and a T-shaped chute communicated with the upper wall is arranged at the center of the right side wall at the other end of the feeding assembly, and the feeding assembly is movably arranged on the mounting frame.
Preferably, the load control structure includes: the device comprises a touch controller, a bracket, a gas detector, a warm lamp, an observation window and a pair of electromagnetic valves with the same structure;
the touch controller is fixedly arranged on the upper wall of the incubator and positioned at the left end, the bottom end of the bracket is fixedly welded on the inner lower wall of the incubator, and is positioned on the central line of the right end, three grooves with the same structure are equidistantly arranged at the central part of the inner upper wall of the bottom end of the bracket, three placing through holes which have the same structure and respectively correspond to the grooves are arranged at the center of the upper wall of the top end of the groove at equal intervals, the gas detector is fixedly arranged on the rear side wall of the right end in the incubator, the warm light is fixedly arranged on the right side wall in the incubator, and is positioned near the top end, the observation window is fixedly embedded on the front wall of the incubator and positioned above the central line of the right end, one end of one electromagnetic valve is fixedly embedded in the upper wall of the incubator and positioned at the right end, and one end of the other electromagnetic valve is fixedly embedded in the front wall of the incubator and positioned at the right end and below the observation window.
Preferably, the bracket is of a concave structure.
Preferably, the gas detector is a multifunction electronic detector.
Preferably, the mounting frame is of an L-shaped structure, and one end of the mounting frame is of a cylindrical structure.
Preferably, the dispensing assembly includes: the auxiliary arm, the clamping claw, the third electric push rod, the test tube and the gasket;
one end of the auxiliary wall is movably embedded in a sliding groove at the other end of the mounting frame, the clamping jaw is fixedly welded at the other end of the auxiliary wall, one end of the third electric rod is fixedly arranged on an upper wall at one end of the mounting frame and located on a central line, the telescopic end of the third electric rod is fixedly welded on the upper wall at one end of the auxiliary wall, the test tube can be detachably clamped on the clamping jaw, and the gasket is movably sleeved at one end of the test tube and located between one end of the test tube and the upper wall of the clamping jaw.
Preferably, the auxiliary arm is of a T-shaped structure.
Preferably, the clamping jaw is of a U-shaped structure.
The invention has the beneficial effects that:
1. according to the invention, after the cell sap is placed in the test tube, the door body can be opened, and the cell sap is placed on the placing structure for preliminary placing, so that a human body is prevented from contacting an internal culture chamber, and the door body is closed;
2. according to the invention, after the preliminarily put test tubes are isolated and sterilized by the isolation structure, the sterilized test tubes can be put on the bracket in the bearing structure by the putting structure, and the test tubes are automatically put in a closed manner in the process, so that the contact of cell sap with the outside can be thoroughly avoided;
3. the invention can connect external equipment through the bearing control structure, for example: carbon dioxide supply and the like are used for controlling the environment in the simulated incubator so as to ensure normal culture and division of the cell sap; the device integrally realizes sterilization, putting and environment simulation for cell culture; not only can avoid external factors to influence the experiment, simultaneously the cost is lower, the control operation of being convenient for.
Drawings
FIG. 1 is a schematic view of the mounting structure of the present invention;
FIG. 2 is a schematic view of the split structure of the present invention;
FIG. 3 is a schematic view of the external structure of the present invention;
fig. 4 is a schematic diagram of a partially enlarged structure a of the present invention.
In the figure: 1. incubator, 2, isolation structure, 21, baffle, 22, sealing strip, 23, baffle, 24, first electric putter, 25, sterilamp, 3, put in the structure, 31, a main part section of thick bamboo, 32, second electric putter, 33, a carrier section of thick bamboo, 34, spacing post, 35, the motor, 36, the mounting bracket, 37, put in the subassembly, 371, the auxiliary arm, 372, the gripper jaw, 373, third electric putter, 374, the test tube, 375, the packing ring, 4, bear the weight of control structure, 41, touch controller, 42, the bracket, 43, the gas detector, 44, the warm light, 45, the observation window, 46, the solenoid valve, 5, the door body.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a stem cell culture apparatus for cell genetic engineering, comprising: an incubator 1; an isolation structure 2 fixedly disposed in the incubator 1 and located at a central portion; the throwing structure is fixedly arranged on the inner lower wall of the incubator 1 and is positioned on the central line of the left end; the bearing control structure 4 is fixedly arranged in the incubator 1 and is positioned at the right end; further comprising: the incubator 1 is of a rectangular box body structure without a left side wall, and one end of the door body is movably arranged on the rear side wall edge of the left end of the incubator 1; an isolation structure 2, comprising: the device comprises a partition plate 21, a sealing strip 22, a baffle plate 23, a first electric push rod 24 and a plurality of sterilizing lamps 25 with the same structure; the upper end and the lower end of a baffle plate 21 are respectively fixedly welded on the upper side wall and the lower side wall in the incubator 1, the baffle plate is positioned at the central part, the central part of the top end of the baffle plate is provided with a throwing opening, a sealing strip 22 is fixedly arranged on the left side wall of the baffle plate 21 and positioned at the throwing opening, one end of a baffle plate 23 is movably arranged on the left side wall of the baffle plate 21 and positioned on a central line, the other end of the baffle plate is arranged on the central line in a knocking way, one end of a first electric push rod 24 is movably connected to the left side wall of the baffle plate 23 and positioned at the central part, the other end of the first electric push rod is movably arranged on the central line of the lower wall in the incubator 1, and a; the isolation structure 2 can effectively sterilize and prevent bacteria from entering to influence culture.
The following electric devices have the following types and functions:
a first electric push rod: it is prior art, as long as the electric putter that is applicable to this scheme all can use.
A second electric push rod: it is prior art, as long as the electric putter that is applicable to this scheme all can use.
A third electric push rod: it is prior art, as long as the electric putter that is applicable to this scheme all can use.
A fourth electric push rod: it is prior art, as long as the electric putter that is applicable to this scheme all can use.
A motor: it is prior art, as long as the motor that is applicable to this scheme all can use.
As a preferred scheme, further, the throwing structure comprises: the device comprises a main body cylinder 31, a second electric push rod 32, a bearing cylinder 33, a pair of limiting columns 34 with the same structure, a motor 35, a mounting frame 36 and a releasing assembly 37;
the main body cylinder 31 is of a cylindrical structure without an upper wall, the left side wall and the right side wall of the main body cylinder are both provided with clamping grooves with the same structure and mutually symmetrical, one of the second electric push rods 32 is fixedly embedded in the main body cylinder 31, the bearing cylinder 33 is of a cylindrical structure without an upper wall, the bearing cylinder 33 is fixedly arranged on the telescopic end of the second electric push rod 32, the lower wall of the bearing cylinder is attached to the upper wall edge of the main body cylinder 31, the left side wall and the right side wall of the bearing cylinder are both provided with matching grooves with the same structure, one end of a pair of limiting columns 34 is respectively fixedly welded on the lower wall of the bearing cylinder 33 and respectively positioned at the left end and the right end in mutual symmetry, the other end of the limiting columns is respectively movably inserted in the clamping grooves of the main body cylinder 31, the motor 35 is movably embedded in the bearing cylinder 33, the left side wall and the right side wall of the bearing cylinder are both fixedly provided with matching blocks and respectively movably embedded in, and a T-shaped chute communicated with the upper wall is arranged at the central part of the right side wall at the other end of the bracket, and the throwing component 37 is movably arranged on the mounting frame 36.
Preferably, the load control structure 4 further includes: a touch controller 41, a bracket 42, a gas detector 43, a warm light 44, an observation window 45, and a pair of electromagnetic valves 46 having the same structure;
the touch controller 41 is fixedly arranged on the upper wall of the incubator 1 and is positioned at the left end, the bottom end of the bracket 42 is fixedly welded on the inner lower wall of the incubator 1, and is positioned on the central line of the right end, three grooves with the same structure are arranged at the central position of the inner upper wall of the bottom end of the bracket 42 at equal intervals, three placing through holes with the same structure and corresponding to the grooves are arranged at the center of the upper wall of the top end of the incubator at equal intervals, a gas detector 43 is fixedly arranged on the rear side wall of the right end in the incubator 1, a warm light 44 is fixedly arranged on the right side wall in the incubator 1, and is positioned near the top end, the observation window 45 is fixedly embedded on the front wall of the incubator 1 and positioned above the central line of the right end, one end of one electromagnetic valve 46 is fixedly embedded in the upper wall of the incubator 1 and is positioned at the right end, one end of the other electromagnetic valve 46 is fixedly embedded in the front wall of the incubator 1 and is positioned at the right end and below the observation window 45.
Preferably, the holder 42 is a concave structure to hold the test tube 374.
Preferably, the gas detector 43 is a multi-function electronic detector designed to monitor the internal environment.
Preferably, the mounting bracket 36 is an L-shaped structure, and one end of the mounting bracket is a cylindrical structure, so as to meet design requirements and facilitate installation and rotation.
Preferably, the dispensing assembly 37 further includes: an auxiliary arm 371, a gripper 372, a third electric push rod 373, a test tube 374, and a washer 375;
one end of the auxiliary wall is movably embedded in a sliding groove at the other end of the mounting frame 36, the clamping claw 372 is fixedly welded at the other end of the auxiliary wall, one end of the third electric lever is fixedly arranged on an upper wall at one end of the mounting frame 36 and is located on a central line, the telescopic end of the third electric lever is fixedly welded on an upper wall at one end of the auxiliary arm 371, the test tube 374 is detachably clamped on the clamping claw 372, and the gasket 375 is movably sleeved on one end of the test tube 374 and is located between one end of the test tube 374 and the upper wall of the clamping claw 372.
Preferably, the auxiliary arm 371 is a T-shaped structure to facilitate the limiting movement.
Preferably, the gripping claws 372 have a U-shaped configuration, so that the test tube 374 is inserted.
The detailed connection means is a technique known in the art, and the following mainly describes the working principle and process, and the specific operation is as follows.
Example (b): 1-4 of the specification, firstly, the gasket 375 is sleeved on one end of the test tube 374, then after the cell sap is placed in the test tube 374, the door body of the incubator 1 can be opened, the test tube 374 is placed in the putting structure, the clamping claws 372 in the putting assembly 37 are prevented from slipping by means of the gasket 375 for preliminary putting, the human body is prevented from contacting the internal culture chamber, after the door body is closed, the equipment is electrified, the sterilization lamp 25 is controlled to work through the touch control screen in the bearing control structure 4, and the baffle 23 is attached to the sealing strip 22 for sealing and isolation so as to perform sterilization; secondly, after the baffle 23 is turned leftwards and kept horizontal by controlling the contraction of the first electric push rod 24 in the isolation structure 2, the second electric push rod 32 in the main body cylinder 31 is controlled to extend to enable the bearing cylinder 33 to rise in a limiting way through the limiting column 34, the mounting frame 36 is driven to rise, the test tube 374 is lifted to pass through the throwing opening of the partition plate 21, the motor 35 is controlled to rotate to drive the mounting frame 36 to rotate 180 degrees to enable the test tube 374 to align with the throwing opening of the partition plate 21, then the third electric push rod 373 is controlled to extend to enable the auxiliary arm 371 to slide in the mounting frame 36, the test tube 374 on the clamping claw 372 is driven to pass through the partition plate 21 and then to be positioned above the bracket 42, according to the throwing requirement, the motor 35 is controlled to rotate again to a certain extent, the test tube 374 is positioned right above the held through hole in cooperation with the auxiliary arm 371, finally the first electric push rod 24 is, the principle is the same as the principle, the control is opposite, after the mounting frame 36 and the bearing cylinder 33 are reset, the baffle plate 23 is closed for sealing; finally, the temperature can be controlled by the warm lamp 44, and the culture condition gas such as carbon dioxide can be supplied by connecting the electromagnetic valve 46 with external equipment, and the concentration of the internal environment gas can be detected by the gas detector 43, and can be observed by the observation window 45.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. A stem cell culture apparatus for cell genetic engineering, comprising:
an incubator (1);
an isolation structure (2) fixedly arranged in the incubator (1) and positioned at the central part;
the throwing structure is fixedly arranged on the inner lower wall of the incubator (1) and is positioned on the central line of the left end;
the bearing control structure (4) is fixedly arranged in the incubator (1) and is positioned at the right end;
further comprising: the incubator (1) is of a rectangular box structure without a left side wall, and one end of the door body is movably arranged on the rear side wall edge of the left end of the incubator (1);
the isolation structure (2) comprises: the sterilization lamp comprises a partition plate (21), a sealing strip (22), a baffle plate (23), a first electric push rod (24) and a plurality of sterilization lamps (25) with the same structure;
the upper end and the lower end of the partition plate (21) are fixedly welded to the upper side wall and the lower side wall in the incubator (1) respectively, the partition plate is located at the central position, a throwing opening is formed in the central position of the top end of the partition plate, the sealing strip (22) is fixedly arranged on the left side wall of the partition plate (21) and located at the throwing opening, one end of the baffle plate (23) is movably arranged on the left side wall of the partition plate (21) and located on a central line, the other end of the baffle plate is arranged on the throwing opening in a knocking mode, one end of the first electric push rod (24) is movably connected to the left side wall of the baffle plate (23) and located at the central position, the other end of the first electric push rod is movably arranged on the central line of the inner lower wall of the incubator (1), and the sterilization lamps (25) are fixedly arranged on.
2. The stem cell culture apparatus for cell genetic engineering according to claim 1, wherein: the delivery structure comprises: the device comprises a main body cylinder (31), a second electric push rod (32), a bearing cylinder (33), a pair of limiting columns (34) with the same structure, a motor (35), a mounting frame (36) and a releasing assembly (37);
the main body cylinder (31) is of a cylindrical structure without an upper wall, the left side wall and the right side wall of the main body cylinder are both provided with clamping grooves with the same structure and are symmetrical with each other, one of the second electric push rods (32) is fixedly embedded in the main body cylinder (31), the bearing cylinder (33) is of a cylindrical structure without an upper wall, the bearing cylinder (33) is fixedly arranged on the telescopic end of the second electric push rod (32), the lower wall of the bearing cylinder is attached to the upper wall edge of the main body cylinder (31), the left side wall and the right side wall of the bearing cylinder are both provided with engagement grooves with the same structure, one end of each limiting column (34) is fixedly welded on the lower wall of the bearing cylinder (33) and is respectively positioned at the left end and the right end of the limiting column to be symmetrical with each other end, the other end of each limiting column is movably inserted in the clamping grooves of the main body cylinder (31), the motor (35) is movably embedded in the bearing cylinder (33), and the left side wall and, one end of the mounting frame (36) is movably sleeved at the top end of the bearing cylinder (33) and fixedly connected to the driving end of the motor (35), a T-shaped chute communicated with the upper wall is formed in the center of the right side wall of the other end of the mounting frame, and the throwing component (37) is movably arranged on the mounting frame (36).
3. The stem cell culture apparatus for cell genetic engineering according to claim 1, wherein: the load control structure (4) comprising: a touch controller (41), a bracket (42), a gas detector (43), a warm light (44), an observation window (45) and a pair of electromagnetic valves (46) with the same structure;
the touch controller (41) is fixedly arranged on the upper wall of the incubator (1) and positioned at the left end, the bottom end of the bracket (42) is fixedly welded on the inner lower wall of the incubator (1) and positioned on the central line of the right end, three grooves with the same structure are equidistantly arranged at the central part of the inner upper wall of the bottom end of the bracket (42), three placing through holes with the same structure and corresponding to the grooves are equidistantly arranged at the central part of the upper wall of the top end of the bracket, the gas detector (43) is fixedly arranged on the rear side wall of the inner right end of the incubator (1), the warm lamp (44) is fixedly arranged on the inner right side wall of the incubator (1) and positioned close to the top end, the observation window (45) is fixedly embedded in the front wall of the incubator (1) and positioned above the central line of the right end, one end of one electromagnetic valve (46) is fixedly embedded in the upper wall of the incubator (1), and is positioned at the right end, wherein one end of the other electromagnetic valve (46) is fixedly embedded in the front wall of the incubator (1) and is positioned at the right end and below the observation window (45).
4. The stem cell culture apparatus for cell genetic engineering according to claim 3, wherein: the bracket (42) is of a concave structure.
5. The stem cell culture apparatus for cell genetic engineering according to claim 3, wherein: the gas detector (43) is a multi-function electronic detector.
6. The stem cell culture apparatus for cell genetic engineering according to claim 2, wherein: the mounting frame (36) is of an L-shaped structure, and one end of the mounting frame is of a cylindrical structure.
7. The stem cell culture apparatus for cell genetic engineering according to claim 2, wherein: -said dosing assembly (37) comprising: an auxiliary arm (371), a clamping claw (372), a third electric push rod (373), a test tube (374) and a gasket (375);
one end of the auxiliary wall is movably embedded in a sliding groove at the other end of the mounting frame (36), the clamping claw (372) is fixedly welded at the other end of the auxiliary wall, one end of the third electric rod is fixedly arranged on an upper wall at one end of the mounting frame (36) and is positioned on a central line, the telescopic end of the third electric rod is fixedly welded on an upper wall at one end of the auxiliary arm (371), the test tube (374) can be detachably clamped on the clamping claw (372), and the gasket (375) is movably sleeved on one end of the test tube (374) and is positioned between one end of the test tube (374) and the upper wall of the clamping claw (372).
8. The stem cell culture apparatus for cell genetic engineering according to claim 7, wherein: the auxiliary arm (371) is of a T-shaped structure.
9. The stem cell culture apparatus for cell genetic engineering according to claim 7, wherein: the clamping claw (372) is of a U-shaped structure.
CN202010765070.1A 2020-08-03 2020-08-03 Stem cell culture equipment for cell genetic engineering Withdrawn CN111979123A (en)

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CN202010765070.1A CN111979123A (en) 2020-08-03 2020-08-03 Stem cell culture equipment for cell genetic engineering

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CN202010765070.1A CN111979123A (en) 2020-08-03 2020-08-03 Stem cell culture equipment for cell genetic engineering

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113667602A (en) * 2021-08-03 2021-11-19 广东省人民医院 High-purity stem cell culture automation equipment

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113667602A (en) * 2021-08-03 2021-11-19 广东省人民医院 High-purity stem cell culture automation equipment

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Application publication date: 20201124