CN212357268U - Virus culture isolator - Google Patents

Abstract

The utility model provides a virus culture isolator, belonging to the technical field of biological medical treatment, comprising a transfer bin, an experiment bin, a culture bin and an air circulation device, wherein the experiment bin is communicated with the transfer bin; the culture bin is communicated with the experiment bin; the transfer storehouse the experiment storehouse and cultivate and equally divide on the storehouse and do not be equipped with the air inlet valve, air circulation device respectively with the transfer storehouse experiment storehouse and cultivate the storehouse and connect, air circulation device and external intercommunication, the transfer storehouse the experiment storehouse with cultivate the storehouse all with the help of the air inlet valve with air circulation device constitutes one-way negative pressure wind path, air circulation device be used for with the transfer storehouse experiment storehouse and cultivate the interior air filtration back discharge external world of storehouse. The utility model provides a virus culture isolator can guarantee aseptic experimental environment, prevents that the combustion gas from having danger, guarantees operator's life safety.

Description

Virus culture isolator

Technical Field

The utility model belongs to the technical field of the biological medical treatment, more specifically say, relate to a virus culture isolator.

Background

According to the infectivity and the hazard of infectious pathogens, the biosafety laboratory is internationally divided into four biosafety levels of P1, P2, P3 and P4, and the higher the biosafety level is, the higher the hazard degree of the corresponding experimental virus is.

Substances which are subjected to experimental research on biological safety grades of P3 and P4 are extremely dangerous and fatal toxic substances, most of the existing isolators are used in laboratories with lower biological safety grades such as P1 and P2, and the isolation effect is not ideal for the highly fatal viruses, so that the isolators cannot be applied to experiments with biological safety grades of P3 and P4.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a virus culture isolator aims at solving current isolator and keeps apart the effect unsatisfactory to highly fatal virus, can't be applied to the experiment of P3, P4 biological safety level.

In order to achieve the above object, the utility model adopts the following technical scheme: provided is a virus culture isolator comprising:

a transfer bin for transferring the material,

the experiment bin is communicated with the transfer bin and is used for carrying out experiment operation on the materials;

the culture bin is communicated with the experiment bin and is used for culturing the virus; air inlet valves are respectively arranged on the transfer bin, the experiment bin and the culture bin; and

the air circulating device is respectively connected with the transfer bin, the experiment bin and the culture bin and is communicated with the outside; the transfer bin, the experiment bin and the culture bin form a one-way negative pressure air path by means of the air inlet valve and the air circulating device, and the air circulating device is used for filtering air in the transfer bin, the experiment bin and the culture bin and then discharging the filtered air to the outside.

As another embodiment of the present application, circulation valves are further respectively disposed between the transfer bin, the experiment bin and the cultivation bin and between the air circulation devices, and the circulation valves are used for forming a closed circulation air path between the air circulation devices and the transfer bin, the experiment bin and the cultivation bin.

As another embodiment of the present application, the air circulation device comprises three air circulation assemblies, three lower portions of the air circulation assemblies are respectively connected to the transfer chamber, the experiment chamber and the culture chamber, and three upper portions of the air circulation assemblies are connected to the transfer chamber, the experiment chamber and the culture chamber through the circulation valves.

As another embodiment of the present application, the air circulation assembly includes:

the box body is respectively communicated with the transfer bin, the experiment bin and the culture bin; an exhaust valve is arranged at the top of the box body;

the first filter is arranged inside the box body;

the second filter is arranged in the box body and is positioned at the top of the first filter; and

and the exhaust fan is arranged in the box body, corresponds to the exhaust valve and is used for exhausting the air flowing out of the second filter to the outside.

As another embodiment of the present application, the transfer cartridge comprises:

a transfer drum; and

the transfer box is in butt joint with the transfer barrel and is respectively communicated with the air circulation device and the experiment bin;

and a transfer valve is arranged between the transfer barrel and the transfer box.

As another embodiment of the present application, the culture chamber comprises:

the bin body is communicated with the experiment bin; and

the incubator is in butt joint with the bin body, is arranged between the bin body and the air circulation device and is respectively communicated with the bin body and the air circulation assembly.

As another embodiment of the present application, the transfer box, the experiment chamber and the chamber body each comprise:

the first bin is communicated with the top of the air circulating device, the air inlet valve is arranged at the top of the first bin, and the circulating valve is arranged between the first bin and the air circulating device; and

the second bin is positioned at the bottom of the first bin, and the bottom of the second bin is communicated with the bottom of the air circulating device;

the flow equalizing film is arranged between the first bin and the second bin; and

the air feeder is arranged at the top of the flow equalizing film, and the air blowing port is vertical to the flow equalizing film.

As another embodiment of this application, the transmission storehouse with be equipped with first wall door between the experiment storehouse, the experiment storehouse with cultivate to be equipped with the second between the storehouse and cut off the door, first wall door with divide equally on the second cuts off the door and do not be equipped with the sealing washer.

As another embodiment of the application, the transfer chamber, the experiment chamber and the culture chamber are all provided with glass panels which can be opened and closed, and the outside of the glass panels is connected with lighting equipment.

As another embodiment of the present application, probes for monitoring the concentration of the sterilization gas are disposed in the transfer chamber, the experiment chamber and the culture chamber.

The utility model provides a virus culture isolator's beneficial effect lies in: compared with the prior art, the utility model puts the instruments to be used into the isolator before using, and fills the sterilizing gas into the transferring bin, the experiment bin and the cultivating bin for sterilizing, closes the air inlet valve, and discharges the sterilizing gas from the air circulating device after the sterilizing gas flows into the transferring bin, the experiment bin and the cultivating bin; the air inlet valve is opened, so that the air flowing out from the air circulation device is faster than the air inlet speed at the air inlet valve, the inside of the transfer bin, the experiment bin and the culture bin reaches a specified negative pressure state after the air circulation device runs for a period of time, then materials are transferred into the experiment bin through the transfer bin, experiment operation is carried out in the experiment bin, and a sample after operation is put into the culture bin for culture. The utility model discloses virus culture isolator is in the experimentation, and the isolator is inside negative pressure state, and the air can only get into the isolator from the external world, and the air in the isolator can not spill over the external world from the air inlet valve, has effectively prevented leaking of virus, lets in new trend to the isolator endogenesis through the air inlet valve constantly, guarantees aseptic, clean experimental environment in the isolator, prevents that other impurity from making the virus of waiting to test produce the variation, influences the experimental result; meanwhile, gas generated in the experiment process enters the air circulation assembly to be filtered and then is discharged to the outside, so that gas with harmful substances generated in the experiment can be effectively purified, the discharged gas is prevented from being dangerous, and the life safety of an operator is ensured.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic front view of a virus culture isolator according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a side view of a virus culture isolator according to an embodiment of the present invention;

FIG. 3 is a schematic top view of the virus culture isolator according to the embodiment of the present invention;

fig. 4 is a flow chart illustrating the replacement of the filter according to the embodiment of the present invention.

In the figure: 1. a transfer bin; 101. a transfer drum; 102. a transfer box; 112. a first chamber; 122 a second chamber; 132. flow equalizing membrane; 142. a blower; 2. an experiment bin; 3. a culture bin; 301. a bin body; 302. an incubator; 4. an air circulation assembly; 401. a box body; 402. a first filter; 403. a second filter; 404. An exhaust fan; 5. an exhaust valve; 6. an air inlet valve; 7. a circulation valve; 8. a transfer valve; 9. a first partition door; 10. a second partition door; 11. a seal ring; 12. a glass panel; 13. a lighting device; 14. operating gloves; 15. a support; 16. a drain valve; 17. a touch display screen; 18. electronic door handles.

Detailed Description

In order to make the technical problem, technical solution and advantageous effects to be solved by the present invention more clearly understood, the following description is given in conjunction with the accompanying drawings and embodiments to illustrate the present invention in further detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Referring to fig. 1 to 3 together, the virus culture isolator provided by the present invention will now be described. The virus culture isolator comprises a transfer bin 1, an experiment bin 2, a culture bin 3 and an air circulating device, wherein the transfer bin 1 is used for transferring materials; the experiment bin 2 is communicated with the transfer bin 1 and is used for carrying out experiment operation on materials; the culture bin 3 is communicated with the experiment bin 2 and is used for culturing the virus; the transfer bin 1, the experiment bin 2 and the culture bin 3 are respectively provided with an air inlet valve 6, the air circulation device 4 is respectively connected with the transfer bin 1, the experiment bin 2 and the culture bin 3 and is communicated with the outside, the transfer bin 1, the experiment bin 2 and the culture bin 3 form a one-way negative pressure air path by virtue of the air inlet valve 6 and the air circulation device, and the air circulation device is used for filtering air in the transfer bin 1, the experiment bin 2 and the culture bin 3 and then discharging the filtered air to the outside.

Compared with the prior art, the virus culture isolator provided by the utility model is characterized in that before use, the instruments to be used and the like are put into the isolator, the sterilizing gas is filled into the transmission bin 1, the experiment bin 2 and the culture bin 3 for sterilization, the air inlet valve 6 is closed, and the sterilizing gas flows into the transmission bin 1, the experiment bin 2 and the culture bin 3 and then is discharged from the air circulation device; open air inlet valve 6, the rate that makes the air follow air cycle device outflow is greater than the air inlet rate of air inlet valve 6 department, and the inside negative pressure state that reaches in transmission storehouse 1, experiment storehouse 2 and the cultivation storehouse 3 this moment, then through transmission storehouse 1 to the interior transmission material of experiment storehouse 2, carry out the experiment operation in experiment storehouse 2, the sample after the operation is put into and is cultivateed in storehouse 3. The utility model discloses virus cultivation isolator is in the experimentation, and the isolator is inside to be negative pressure state, and the air can only get into the isolator from the external world, and the air in the isolator can not spill over the external world from intake valve 6, has effectively prevented leaking of virus, lets in new trend to the isolator endogenesis incessantly through intake valve 6, guarantees aseptic, clean experimental environment in the isolator, prevents that other impurity from making the virus of waiting to test produce the variation, influences the experimental result; meanwhile, gas generated in the experiment process enters the air circulation assembly 4 to be filtered and then is discharged to the outside, so that gas with harmful substances generated in the experiment process can be effectively purified, the discharged gas is prevented from being dangerous, and the life safety of an operator is ensured.

It should be noted that the sterilizing gas can be filled from the air inlet valve 6, and the air inlet valve 6 is immediately closed after the sterilizing gas is filled from the air inlet valve 6; the sterilizing gas may be supplied from the glass panels 12 of the transfer chamber 1, the experiment chamber 2 and the incubation chamber 3, respectively, and the glass panels are closed immediately after the sterilizing gas is supplied from the glass panels 12.

As a specific embodiment of the virus culture isolator provided by the present invention, please refer to fig. 2, the transfer bin 1, the experiment bin 2, the culture bin 3 and the air circulation device are further respectively provided with a circulating valve 7, and the circulating valve 7 is used for forming a closed circulation wind path with the air circulation device and the transfer bin 1, the experiment bin 2 and the culture bin 3. In a disinfection state, the circulating valve 7 is opened, and the sterilization gas filled into the isolator circularly flows between the isolator and the air circulating device, so that self-circulation air supply between the isolator and the air circulating assembly 4 can be realized, and the disinfection efficiency of the isolator is improved; by closing the two remaining circulation valves 7, the opening of only one circulation valve 7 makes it possible to achieve a separate sterilization of the transfer chamber 1 or the test chamber 2 or the incubation chamber 3.

In this embodiment, the circulating valve 7 may be a common valve or a proportional regulating valve. When the circulating valve 7 is a common valve, whether the sterilizing gas circulates or not is switched by adjusting the opening and closing of the circulating valve 7 during sterilization; when the circulating valve 7 is a proportional regulating valve, the circulating speed of the sterilizing gas can be controlled by regulating the opening degree of the circulating valve 7.

As a specific implementation manner of the virus culture isolator provided by the present invention, please refer to fig. 3, the air circulation device includes three air circulation assemblies 4, the lower portions of the three air circulation assemblies 4 are respectively connected with the transfer bin 1, the experiment bin 2 and the culture bin 3, and the upper portions of the three air circulation assemblies 4 are respectively connected with the transfer bin 1, the experiment bin 2 and the culture bin 3. Can realize the independent disinfection to transfer storehouse 1, experiment storehouse 2 and cultivation storehouse 3 through three air cycle subassembly 4, for example, transfer storehouse 1 all will advance it to disinfect once more after the material of every transfer, can disinfect it alone through air cycle subassembly 4 that transfer storehouse 1 corresponds this moment, need not disinfect once more to the isolator is whole again, has increased sterile flexibility, compares and has reduced the gaseous use of sterilization in whole disinfection, the cost is reduced.

As a specific embodiment of the virus culture isolator provided by the present invention, please refer to fig. 1 to 2, the air circulation assembly 4 includes a box 401, a first filter 402, a second filter 403 and an exhaust fan 404, the box 401 is respectively communicated with the transfer bin 1, the experiment bin 2 and the culture bin 3; an exhaust valve 5 is arranged at the top of the box body 401; the first filter 402 is provided inside the case 401; the second filter 403 is disposed inside the case 401 and on top of the first filter 402; the exhaust fan 404 is disposed inside the case 401 and corresponds to the exhaust valve 5, and exhausts the air flowing out of the second filter 403 to the outside. In an experimental state, the air inlet valve 6 and the air outlet valve 5 are in an open state, the circulating valve 7 is closed, fresh air enters the interior of the transfer bin 1 through the top of the transfer bin 1, enters the bottom of the box body 401 at the bottom of the transfer bin 1, sequentially passes through the first filter 402 and the second filter 403, and flows out of the air outlet valve 5 under the assistance of the air outlet fan 404, and the air outlet fan 404 in the air circulating assembly 4 can promote the circulation of air to ensure a sterile negative pressure state in the transfer bin 1; in a disinfection state, the air inlet valve 6 and the air outlet valve 5 are in a closed state, the circulating valve 7 is in an open state, sterilizing gas (hydrogen peroxide gas can be filled in the isolator), enters the box body 401 from the bottom of the transfer bin 1 under the assistance action of the air outlet fan 404, sequentially flows through the first filter 402 and the second filter 403, flows to the top of the box body 401, and enters the top of the transfer bin 1 through the circulating valve 7, so that the circulating disinfection is realized, and the disinfection efficiency is improved. The air circulation path between the air circulation assembly 4 and the experiment bin 2 or the culture bin 3 is the same as the air circulation path between the air circulation assembly 4 and the transfer bin 1 in a disinfection state or an experiment state, and the first filter 402 and the second filter 403 in the air circulation assembly 4 can carry out two-stage filtration on air flowing out of the isolator, so that harmful substances are effectively prevented from flowing out, and the safety of an operator is guaranteed.

In this embodiment, the first filter 402 and the second filter 403 are both advanced filters, which can effectively trap viruses in the air discharged from the isolator. The exhaust fan 404 is a stepless speed regulating fan which can regulate the speed steplessly and automatically control the air outlet speed so as to change the air outlet speed and change the pressure inside the isolator, but the air outlet speed of the exhaust fan 404 is always greater than the air inlet speed of the air inlet valve 6 in an experimental state; the stepless speed regulation of the exhaust fan 404 can also adapt to different experimental states, for example, when the air circulation fluidity is needed to be strong in the experimental state process, the air outlet speed of the exhaust fan 404 is increased, when the air circulation fluidity is needed to be weak in the experimental state process, the air outlet speed of the exhaust fan 404 is decreased, but the air outlet speed of the exhaust fan 404 is always greater than the air inlet speed of the air inlet valve 6 in the experimental state.

As a specific implementation mode of the virus culture isolator provided by the utility model, the exhaust valve 5 and the air supply valve are proportional control valves. The opening degree of the exhaust valve 5 and the opening degree of the air inlet valve 6 can be freely adjusted, the opening degree of the exhaust valve 5 is adjusted to be larger than that of the air inlet valve 6, the air outlet rate can be larger than the air inlet rate, the isolator can reach a negative pressure state, the air in the isolator can be effectively prevented from flowing out of the air inlet valve 6 due to the fact that the isolator is lower than the external pressure in the negative pressure state, the virus leakage is effectively blocked, and the safety of an operator is guaranteed.

As a specific embodiment of the virus culture isolator provided by the present invention, please refer to fig. 1 and 3, the transfer chamber 1 includes a transfer barrel 101 and a transfer box 102, the transfer box 102 is connected to the transfer barrel 101, and the transfer box 102 is connected to the air circulation device and the experiment chamber 2; a transfer valve 8 is provided between the transfer barrel 101 and the transfer box 102. The transmission bucket 101 is RTP transmission bucket (real-time aseptic transmission bucket), and transfer valve 8 is RTP transmission valve (real-time aseptic transmission valve), can realize the aseptic transmission of material through RTP transmission bucket and RTP transmission valve, need not frequently carry out sterilization again, can also prevent to bring external thallus into the isolator in, guarantee inside sterile condition. When the articles with smaller volume need to be transferred, the articles are transferred to the transfer box 102 through the transfer barrel 101 and then enter the experiment bin 2 for experiment operation; when large-volume articles need to be transferred, the articles are put into the transfer box 102 by pulling the glass panel 12 open, the glass panel 12 is closed, then sterilization treatment is carried out, and then the articles are put into the experiment bin 2 for experiment operation; the transfer barrel 101 and the transfer box 102 of the transfer bin 1 can be suitable for transferring articles with different volumes, wherein the articles with small volumes are transferred without frequent disinfection, and the experimental process is simplified.

As a specific embodiment of the virus culture isolator provided by the present invention, please refer to fig. 1 and 3, the culture chamber 3 includes a chamber body 301 and an incubator 302, the chamber body 301 is communicated with the experiment chamber 2; the incubator 302 is in butt joint with the cabin body 301, and the incubator 302 is arranged between the cabin body 301 and the air circulation device and is respectively communicated with the cabin body 301 and the air circulation component 4.

Traditional cultivation storehouse 3 is generally put into storehouse body 301 with incubator 302 when using, because incubator 302 is outside from taking some spare parts, causes incubator 302 outside to have the dead angle, can not obtain thorough sterilization cleaning process, and then can influence subsequent cultivation process. In this embodiment, article after experiment 2 operation are put into incubator 302 through storehouse body 301, storehouse body 301 and incubator 302 directly adopt flange seal butt joint, the inner chamber of incubator 302 only communicates with the inner chamber of the storehouse body 301, when disinfecting storehouse body 301 and incubator 302, the sterilizing gas only flows through from the inside of storehouse body 301 and incubator 302, when having solved conventional scheme and having put whole incubator 302 into storehouse body 301, the outside process element of incubator and the difficult cleanness in dead angle that the control element led to the fact, difficult sterilization causes the not thorough problem of the inside sterilization of storehouse body 301, further improve the aseptic nature of device, guarantee the accuracy of experimental result.

In this embodiment, be equipped with a plurality of independent inner chambers in the incubator 302, can hold multiunit experimental sample simultaneously, the sample separation that conveniently will not be of the same type is stored, prevents that the sample is chaotic not to seek, guarantees the orderly deposit of sample, conveniently seeks, reduces operator's intensity of labour.

As a specific embodiment of the virus culture isolator provided by the present invention, please refer to fig. 1 to 2, the transfer box 102, the experimental chamber 2 and the chamber body 301 respectively comprise a first chamber 112, a second chamber 122, a flow equalizing membrane 132 and a blower 142, the first chamber 112 is communicated with the top of the air circulation device, the top of the first chamber 112 is provided with an air inlet valve 6, and a circulating valve 7 is arranged between the first chamber 112 and the air circulation device; the second chamber 122 is located at the bottom of the first chamber 112, and the bottom of the second chamber 122 is communicated with the bottom of the air circulation device; the flow equalizing membrane 132 is disposed between the first chamber 112 and the second chamber 122; the blower 142 is arranged on the top of the flow equalizing membrane 132 and the air outlet is arranged perpendicular to the flow equalizing membrane 132. In an experimental state, the air inlet valve 6 is opened, fresh air enters the first chamber 112, under the assistance action of the blower 142, the air flow enters the second chamber 122 from the top of the second chamber 122 uniformly and vertically through the flow equalizing membrane 132, the waste air in the second chamber 122 can be effectively replaced by the air flow entering through the vertical laminar flow, and a sterile and clean operation space in the second chamber 122 is ensured.

In this embodiment, the blower 142 is a stepless speed-regulating blower, which can perform stepless speed regulation and automatically control the air inlet speed, so as to change the pressure inside the isolator, but the air outlet speed of the exhaust blower 404 is always greater than the air inlet speed of the blower 142 in the experimental state; the stepless speed regulation of the blower 142 can also be adapted to different experimental states, for example, when the air circulation fluidity is required to be strong in the experimental state process, the air inlet speed of the blower 142 is increased, and when the air circulation fluidity is required to be weak in the experimental state process, the air inlet speed of the blower 142 is decreased, but the air outlet speed of the exhaust fan 404 is always greater than the air inlet speed of the air inlet fan in the experimental state.

As a specific implementation of the virus culture isolator provided by the utility model, please refer to fig. 1-2, be equipped with high efficiency filter between forced draught blower 142 and the membrane 132 that flow equalizes, high efficiency filter can filter the air that blows out in the forced draught blower 142, carries other bacteriums etc. in the new trend that prevents outside entering, can also prevent to have the bacterium to blow out in the forced draught blower 142, guarantees aseptic, clean operational environment in the second bin 122.

As a specific embodiment of the virus culture isolator provided by the present invention, please refer to fig. 1 and 3, a first partition door 9 is provided between the transmission chamber 1 and the experiment chamber 2, a second partition door 10 is provided between the experiment chamber 2 and the culture chamber 3, and the first partition door 9 and the second partition door 10 are equally provided with a sealing ring 11. In the process that the material enters the experiment bin 2 from the transmission bin 1, the first partition door 9 is opened, the material enters the experiment bin 2, the first partition door 9 is closed, after the experiment bin 2 finishes the experiment operation on the material, the second partition door 10 is opened, the sample is put into the culture bin 3, and the second partition door 10 is closed. First wall door 9 and second wall door 10 can guarantee the relative seal between two adjacent storehouses when not needing the transmission article, and sealing washer 11 can increase the leakproofness that cuts off the door, conveniently disinfects solitary storehouse, prevents inside production cross contamination, improves the accuracy of experimental result.

The sealing ring 11 is an inflatable sealing ring 11, and after the first partition door 9 or the second partition door 10 is closed, the sealing ring 11 is inflated to expand, so that a gap on the first partition door 9 or the second partition door 10 is effectively blocked, and the sealing effect of the sealing ring 11 is optimized.

As a specific embodiment of the virus culture isolator provided by the present invention, please refer to fig. 1 and 3, the transfer chamber 1, the experiment chamber 2 and the culture chamber 3 are all provided with a glass panel 12 capable of being opened and closed, and the outside of the glass panel 12 is connected with an illumination device 13. The glass panel 12 is designed as a sliding door, and the glass panel 12 can conveniently observe the internal operation state under the working state of the isolator; after work is finished and sterilization is carried out, the interior can be cleaned or the interior appliances can be replaced; lighting apparatus 13 has been stopped traditional light and has been installed inside the isolator and produce the problem that the dead angle is good to be washd and disinfect in the outside at the isolator, and lighting apparatus 13 adopts polychrome illumination, can adopt different colours to throw light on to isolator inside under the technological condition of difference, and the running state of suggestion operator isolator that can be audio-visual prevents the maloperation.

In this embodiment, the glass panel 12 is further provided with an air spring, which can assist the opening and closing of the glass panel 12, and is convenient for an operator to operate.

As the utility model provides a specific implementation of isolator is cultivateed to virus, please refer to fig. 1 to fig. 3, install operating gloves 14 on the glass panels 12, operating gloves 14 are used for the experiment operation, realize operating personnel and internal operation space's isolation, transmit storehouse 1 simultaneously, all be equipped with the support 15 that is used for supporting operating gloves 14 in experiment storehouse 2 and the cultivation storehouse 3, support 15 can prevent that operating gloves 14 from hanging down and causing cleanness, the sterilization dead angle on glass panels 12, in the sterilization process, support 15 expandes the gloves surface, it is thorough to the convenient sterilization of inner chamber.

As a specific implementation mode of the virus culture isolator provided by the utility model, the probe used for monitoring the concentration of the sterilization gas is arranged in the transmission bin 1, the experiment bin 2 and the culture bin 3. The probes are respectively arranged in the inner cavities and are gas concentration probes (mainly used for detecting high-concentration gas), so that whether the concentration of the sterilization gas in the inner cavity is qualified or not in the sterilization process can be detected, whether the concentration of the residual sterilization gas in the inner cavity reaches the safe emission standard or not after the sterilization is finished can be judged, and the environment pollution is prevented.

In the embodiment, the virus culture isolator is also provided with a gas discharge pipeline connected with the air circulation device, the gas discharge pipeline is also provided with a probe (mainly used for detecting low-concentration gas), and the probe at the position can judge whether the concentration of residual sterilizing gas in the isolator can reach the virus experiment operation concentration standard after sterilization is finished; the outside of isolator also is installed mainly and is surveyed low concentration gas probe, can judge whether have sterilizing gas to reveal in the sterilization process, prevents to produce harm to the operator.

As a specific embodiment of the virus culture isolator provided by the present invention, please refer to fig. 1 to 2, the bottoms of the transfer bin 1, the experiment bin 2 and the culture bin 3 are all provided with a drain valve 16, which facilitates the drainage after cleaning the inner cavity.

As a specific implementation manner of the virus culture isolator provided by the present invention, please refer to fig. 1, the virus culture isolator further includes a control terminal, which is in communication connection with the transfer bin 1, the experiment bin 2, the culture bin 3 and the air circulation component 4, respectively. The control terminal can be operated through the touch display screen 17, and the display screen is installed at the tail end of the culture bin 3, and the control terminal is used for controlling the lighting device 13, the transfer valve 8, the exhaust valve 5, the intake valve 6, the circulating valve 7, the exhaust fan 404, the blower 142, the glass panel 12, the sealing ring 11, the drain valve 16 and the partition door respectively, and the specific control process is as follows:

the control terminal can control the lighting device 13 to be turned on or turned off, and switch the lighting device 13 to emit light of different colors in different experimental states;

the control terminal can control the opening and closing of the transfer valve 8 and is used for controlling the transfer valve 8 to transfer materials;

the control terminal can control the opening and closing of the exhaust valve 5, can adjust the opening degree of the exhaust valve 5 and controls the air outlet rate;

the control terminal can control the opening and closing of the air inlet valve 6, can adjust the opening degree of the air inlet valve 6 and controls the air inlet speed;

the control terminal can control the opening and closing of the circulating valve 7, can adjust the opening degree of the circulating valve 7 and controls the flow rate of the sterilizing gas in the sterilizing process;

the control terminal can control the opening and closing of the exhaust fan 404, and can adjust the fan rotating speed of the exhaust fan 404 to promote or slow down the circulation of air in the inner cavity;

the control terminal can control the on and off of the blower 142 and can adjust the fan rotating speed of the blower 142 to promote or slow down the circulation of air in the inner cavity;

the electronic door handle 18 is arranged on the glass panel 12, the control terminal can control the opening and closing of the electronic door handle 18 to realize the opening and closing of the glass panel 12, the electronic door handle 18 can also realize automatic locking, whether the glass panel 12 is closed in place or not can be displayed at the control terminal, and a warning can be sent if the glass panel 12 is not closed tightly;

the control terminal can control the opening and closing of the first partition door 9 and the second partition door 10;

when the first partition door 9 or the second partition door 10 is closed, the control terminal controls the corresponding inflating equipment to inflate into the corresponding sealing ring 11, and the inflating equipment is closed after the inflation is finished;

after the device is cleaned, the control terminal controls the drainage valve 16 to be opened, and controls the drainage valve 16 to be closed after drainage is finished;

and the value monitored by the probe is reflected to the control terminal, and when the monitored value exceeds the standard interval, the control terminal sends out an alarm.

The use method of the virus culture isolator comprises the following steps: respectively introducing sterilizing gas into the transfer bin 1, the experiment bin 2 and the culture bin 3, and enabling the sterilizing gas to flow out of the air circulation device under the state that the air inlet valve 6 is closed to realize integral disinfection; the air circulation device exhausts air to the outside, the air inlet valve 6 is opened, the materials in the transfer bin 1, the experiment bin 2 and the culture bin 3 are adjusted to reach a negative pressure state, and the materials are transferred to the experiment bin 2 through the transfer bin 1; sealing the transfer bin 1 and the experiment bin 2, and transferring the next batch of materials after independently sterilizing the transfer bin 1; after the experiment operation is performed in the experiment chamber 2, the sample is placed in the culture chamber 3 for culture. According to the method, the transfer bin 1 is disinfected once again every time the materials are transferred, so that other bacteria are effectively prevented from being brought in when the external materials enter the isolator, and a sterile and clean experimental environment in the experimental process is ensured; the method ensures the continuous circulation of fresh air in the isolator, prevents the virus leakage by using the internal negative pressure state in the circulation state, and ensures the safety of operators.

All reach the negative pressure state in adjusting transfer storehouse 1, experiment storehouse 2 and cultivating the storehouse 3, specifically include: the exhaust rate of the air circulation device is V1, the intake rate of the air inlet valve 6 is V2, and the air circulation device and the air inlet valve 6 are adjusted to ensure that V1 is larger than V2; the transfer bin 1, the experiment bin 2 and the culture bin 3 reach a negative pressure state. Because the isolator is lower than the external pressure in the negative pressure state, the air in the isolator can be effectively prevented from flowing out from the air inlet valve 6, the leakage of viruses is effectively blocked, and the safety of an operator is guaranteed.

Respectively to passing through sterile gas in transfer storehouse 1, experiment storehouse 2 and the cultivation storehouse 3, sterile gas flows out from air cycle device under the state that air inlet valve 6 was closed and realizes whole disinfection, specifically includes: respectively introducing sterilizing gas into the transfer bin 1, the experiment bin 2 and the culture bin 3; opening a circulating valve 7, closing an air inlet valve 6 and an air outlet valve 5, and enabling the sterilizing gas to fully and circularly flow inside until the sterilization is finished; the sterilizing gas is exhausted by opening the exhaust valve 5. The sterilization gas flows between the isolator and the air circulation assembly 4 in a self-circulation mode for sterilization, so that the sterilization efficiency of the isolator is improved, the sterilization gas does not need to be continuously filled again, the use of the sterilization gas is reduced, and the cost is reduced.

Before carrying out disinfection treatment on the transfer bin 1, the experiment bin 2 and the culture bin 3, the inner cavity needs to be cleaned firstly, the water inside the inner cavity is discharged through the drain valve 16, then, instruments, reagents, samples and other materials which are cleaned in advance and pass through sterile detection are put into the inner cavity, the glass panel 12 is closed to be sealed, the operation gloves 14 and the inner cavity of the isolator are respectively pressed, and whether the whole tightness of the operation gloves 14 and the inner cavity is qualified or not is judged according to the pressure change speed.

Need change after high efficiency filter uses many times, and concrete change process is: covering a plastic bag on a door frame of the box door, and opening the box door (a) of the high-efficiency filter; loading a high-efficiency filter to be replaced into a plastic bag fixed on a door frame (b); double sealing the plastic bag, cutting off the plastic bag between two seals, and removing the replaced high-efficiency filter in a sealing way (c); loading a new high-efficiency filter into a new plastic bag and fixing the new high-efficiency filter on a door frame (d); and (e) taking down the last residual plastic bag opening in the new plastic bag, finishing the installation of the new high-efficiency filter, and closing the box door (e). This process effectively prevents the spread of virus that may remain in the high efficiency filter.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims (10)

1. A virus culture isolator, comprising:

a transfer bin for transferring the material,

the experiment bin is communicated with the transfer bin and is used for carrying out experiment operation on the materials;

the culture bin is communicated with the experiment bin and is used for culturing the virus; air inlet valves are respectively arranged on the transfer bin, the experiment bin and the culture bin; and

the air circulating device is respectively connected with the transfer bin, the experiment bin and the culture bin and is communicated with the outside; the transfer bin, the experiment bin and the culture bin form a one-way negative pressure air path by means of the air inlet valve and the air circulating device, and the air circulating device is used for filtering air in the transfer bin, the experiment bin and the culture bin and then discharging the filtered air to the outside.

2. The virus cultivation isolator according to claim 1, wherein circulation valves are respectively disposed between the transfer chamber, the experiment chamber and the cultivation chamber and the air circulation device, and the circulation valves are used to form a closed circulation air path between the air circulation device and the transfer chamber, the experiment chamber and the cultivation chamber.

3. The virus culture isolator according to claim 2, wherein the air circulation means comprises three air circulation modules, lower portions of the three air circulation modules are connected to the transfer chamber, the experiment chamber and the incubation chamber, respectively, and upper portions of the three air circulation modules are connected to the transfer chamber, the experiment chamber and the incubation chamber through the circulation valves.

4. The virus culture isolator of claim 3, wherein the air circulation assembly comprises:

the box body is respectively communicated with the transfer bin, the experiment bin and the culture bin; an exhaust valve is arranged at the top of the box body;

the first filter is arranged inside the box body;

the second filter is arranged in the box body and is positioned at the top of the first filter; and

and the exhaust fan is arranged in the box body, corresponds to the exhaust valve and is used for exhausting the air flowing out of the second filter to the outside.

5. The virus culture isolator of claim 2, wherein the transfer chamber comprises:

a transfer drum; and

the transfer box is in butt joint with the transfer barrel and is respectively communicated with the air circulation device and the experiment bin;

and a transfer valve is arranged between the transfer barrel and the transfer box.

6. The virus culture isolator of claim 5, wherein the culture compartment comprises:

the bin body is communicated with the experiment bin; and

the incubator is in butt joint with the bin body, is arranged between the bin body and the air circulating device and is respectively communicated with the bin body and the air circulating device.

7. The virus culture isolator of claim 6, wherein the transfer box, the experimental chamber, and the chamber body each comprise:

the first bin is communicated with the top of the air circulating device, the air inlet valve is arranged at the top of the first bin, and the circulating valve is arranged between the first bin and the air circulating device; and

the second bin is positioned at the bottom of the first bin, and the bottom of the second bin is communicated with the bottom of the air circulating device;

the flow equalizing film is arranged between the first bin and the second bin; and

the air feeder is arranged at the top of the flow equalizing film, and the air blowing port is vertical to the flow equalizing film.

8. The virus cultivation isolator according to claim 1, wherein a first partition door is provided between the transfer chamber and the experiment chamber, a second partition door is provided between the experiment chamber and the cultivation chamber, and the first partition door and the second partition door are respectively provided with a sealing ring.

9. The virus culture isolator according to claim 1, wherein the transfer chamber, the experiment chamber and the culture chamber are provided with openable and closable glass panels, and an illumination device is connected to the outside of the glass panels.

10. The virus culture isolator of claim 1, wherein probes for monitoring the concentration of sterilizing gas are disposed in the transfer chamber, the test chamber and the incubation chamber.

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