CN211620500U - Unmanned isolation detection system for high-risk pathogenic microorganisms - Google Patents
Unmanned isolation detection system for high-risk pathogenic microorganisms Download PDFInfo
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- CN211620500U CN211620500U CN201922180375.1U CN201922180375U CN211620500U CN 211620500 U CN211620500 U CN 211620500U CN 201922180375 U CN201922180375 U CN 201922180375U CN 211620500 U CN211620500 U CN 211620500U
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Abstract
The utility model belongs to biological safety protection technique and equipment field, the utility model discloses a detection system is kept apart to unmanned high-risk pathogenic microorganism. The device comprises: the device comprises an isolation cabin, a transfer cabin, a pathogen nucleic acid automatic processing and detecting unit, a ventilation and filtration system and a control system; the pathogen nucleic acid automatic processing and detecting unit is arranged in the isolation cabin, the bottom of the isolation cabin is provided with a waste discharge port, the interior of the isolation cabin is provided with an operating glove, one side of the operating glove is communicated with the transfer cabin, and the other side of the operating glove is provided with an equipment door; one side of the transfer cabin is communicated with the isolation cabin, the other side of the transfer cabin is provided with a first airtight door, a second airtight door is arranged on the transfer cabin, and the second airtight door is communicated with the sample inactivation module; the ventilation and filtration system comprises an isolation cabin ventilation and filtration device and a transfer cabin ventilation and filtration device; the isolation cabin ventilation and filtration device is arranged at the top of the isolation cabin, and the transmission cabin ventilation and filtration device is arranged on a window body of the transmission cabin. The device of the utility model has high automation degree and good biological safety.
Description
Technical Field
The utility model relates to a biological safety protection technique and equipment field, concretely relates to detection system is kept apart to unmanned of high risk pathogenic microorganism.
Background
In recent years, serious infectious diseases caused by high-risk pathogenic microorganisms are rough and cause serious harm to human health. In order to reduce the infection risk, the treatment and detection of samples possibly containing high-risk pathogenic microorganisms are generally carried out in a biosafety cabinet of a high-grade biosafety laboratory, and an operator must wear individual protective equipment all the time. Nevertheless, there is still a great risk of exposure to the operator during the entire work process.
The method has the advantages that the method is wide in width of members in China, the number of high-grade biosafety laboratories is not uniformly distributed, especially in remote areas, the method is a natural origin of many infectious disease epidemic situations, the sanitary conditions are backward, the disease control force is very weak, professional personnel and equipment for biosafety defense are lacked, the detection and treatment of high-risk pathogenic microorganisms on the epidemic situation site are difficult to carry out, and the method brings a serious challenge to the site emergency prevention and control of new emergent infectious diseases in China.
At present, traditional pathogen detection mainly depends on the manual work, can only accomplish depending on many laboratory equipment, and whole flow includes operations such as sample, sample inactivation, nucleic acid extraction, nucleic acid detection and save, and the step is loaded down with trivial details, and is consuming time longer, and the exposure risk is higher. Meanwhile, the operation process also comprises the collection and safe treatment of wastes. Although there are many liquid workstations on the market at present that can automatically complete the nucleic acid extraction, they are generally applied in the experimental situation where a single type of specimen is extracted at one time and the amount of the specimen is very large (tens to hundreds), and the extraction process has no strict biological safety isolation measure, and is not suitable for the nucleic acid extraction of the high-risk pathogen sample, and the nucleic acid extraction is only one step in the whole nucleic acid detection process. The existing on-site detection equipment has low automation degree, and is difficult to meet the requirements of full-process automation processing from sample direct to nucleic acid detection. Therefore, most of the existing equipment is positioned in a laboratory for application, the functions are dispersed, the high-level biological protection design is lacked, and the full-flow automatic treatment and detection of the high-risk pathogenic microorganism samples cannot be realized.
Most of domestic and microbial nucleic acid detection equipment aims at common microbial samples, so that no biological protection design aiming at high-risk pathogen microbial nucleic acid detection exists, and the method is not suitable for independently carrying out high-risk pathogen sample nucleic acid detection on infectious disease prevention and control sites or common laboratories.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a detection system is kept apart to unmanned of high risk pathogenic microorganism, the utility model discloses a device degree of automation is high, has good biosafety.
The embodiment of the first aspect of the utility model provides a detection system is kept apart to high-risk pathogenic microorganism unmanned, unmanned isolation detection system include: the device comprises an isolation cabin, a transfer cabin, a pathogen nucleic acid automatic processing and detecting unit, a ventilation and filtration system and a control system;
the pathogen nucleic acid automatic processing and detecting unit is arranged in the isolation cabin, a waste discharge port is arranged at the bottom of the isolation cabin, a waste collecting bag is sleeved at the discharge port, an operating glove is arranged in the isolation cabin, one side of the isolation cabin is communicated with the transfer cabin, and an equipment door is arranged at the other side of the isolation cabin;
one side of the transfer cabin is communicated with the isolation cabin, the other side of the transfer cabin is provided with a first airtight door, one side of the transfer cabin, which is communicated with the isolation cabin, is provided with a second airtight door, and the second airtight door is communicated with a sample inactivation module of the pathogen nucleic acid automatic processing and detecting unit;
the ventilation and filtration system comprises an isolation cabin ventilation and filtration device and a transmission cabin ventilation and filtration device; the isolation cabin ventilation and filtration device is arranged at the top of the isolation cabin, and the transfer cabin ventilation and filtration device is arranged on a window body of the transfer cabin.
Furthermore, the ventilation and filtration device of the transfer cabin comprises an air supply efficient filter, an air exhaust efficient filter and an exhaust fan; a first airtight valve is arranged at an air inlet at the front end of the air supply high-efficiency filter, and a second airtight valve is arranged between the air exhaust high-efficiency filter and the exhaust fan; the air supply high-efficiency filter is arranged on the window body of the transfer cabin, and the air exhaust high-efficiency filter is arranged on the window body of the transfer cabin.
Further, the ventilation and filtration device for the isolation cabin comprises: the air supply high-efficiency filter, the air exhaust high-efficiency filter, the air feeder and the exhaust fan; the air supply high-efficiency filter is arranged at the top of the isolation cabin, and the air exhaust high-efficiency filter is arranged at the top of the isolation cabin; the air supply device is characterized in that a first airtight valve is arranged between the air supply device and the air supply high-efficiency filter, and a second airtight valve is arranged between the exhaust fan and the air exhaust high-efficiency filter.
Furthermore, the control system comprises an isolation cabin automatic control device and a pathogen nucleic acid automatic processing and detecting unit control device.
Furthermore, the isolation cabin automatic control device is arranged at the upper part of the isolation cabin, and the pathogen nucleic acid automatic processing and detecting unit control device is arranged at the upper part of the transfer cabin.
Furthermore, one side of the bottom in the isolation cabin is provided with a local laminar flow air outlet for providing clean air protection for reserving a sample for a nucleic acid sample, and the front end of the laminar flow air outlet is sequentially provided with a high-efficiency filter, a closed valve and a fan.
Furthermore, the cabin body of the isolation cabin is made of a flexible transparent film.
Furthermore, the equipment door is of an airtight zipper type structure.
Furthermore, the pathogen nucleic acid automatic processing and detecting unit comprises a sample inactivation module, a nucleic acid extraction module and a nucleic acid detection module.
Further, the high-risk pathogenic microorganism unmanned isolation detection system comprises a display device for displaying processing and detection results.
The embodiment of the second aspect of the utility model provides an automatic processing and detection method of high-risk pathogenic microorganism, adopt foretell any kind of device to accomplish, include the following step:
putting a reagent required by detection into a corresponding position of the isolation cabin through the transfer cabin;
the original sample is sent into a transfer cabin, and then sent into a pathogen nucleic acid automatic processing and detecting unit of an isolation cabin, and the pathogen nucleic acid automatic processing and detecting unit detects the sample;
and after the sample detection is finished, carrying out gas fumigation on the transfer cabin and the isolation cabin.
Borrow by above-mentioned scheme, the utility model discloses detection system is kept apart to high risk pathogenic microorganism unmanned has following beneficial effect at least:
the utility model discloses an automatic operation mode of detection system is kept apart to unmanned high-risk pathogenic microorganism can improve work efficiency by a wide margin, reduces the requirement to operating personnel professional skill, is favorable to handling and detects the standardization and the objectification of flow, reduces the influence of operating personnel subjective factor to the result. But high-grade biological protection isolation design furthest reduces the leakage risk and the operating personnel's of high-risk sample exposure, makes the utility model discloses become one set of mobilizable high-risk pathogenic microorganism that can independently move and handle and detect the laboratory, be particularly suitable for lacking the mobile application occasion of high-grade biological laboratory facility, but the mobile processing and the prevention and control ability of the dangerous pathogen of real promotion.
Drawings
FIG. 1 is a schematic structural view of an unmanned isolation detection system for high-risk pathogenic microorganisms according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of an embodiment of an automated pathogen nucleic acid processing and detection unit;
FIG. 3 is a schematic view of a connection structure of the sterilizer with the transfer chamber and the isolation chamber according to an embodiment of the present invention;
fig. 4 is a schematic view of a local laminar air outlet structure according to an embodiment of the present invention.
Detailed Description
The present invention is further described in detail below by the following examples so that those skilled in the art can further understand the present invention, but do not constitute any limitation to the present invention.
With reference to fig. 1-4, an unmanned isolation detection system for high risk pathogenic microorganisms comprises: the device comprises an isolation cabin 2, a transfer cabin 1, a pathogen nucleic acid automatic processing and detecting unit 15, a ventilation and filtration system and a control system;
the pathogen nucleic acid automatic processing and detecting unit 15 is arranged inside the isolation cabin 2, a waste discharge port is arranged at the bottom of the isolation cabin 2, the waste discharge port is connected with a transfer sleeve 9, a waste collecting bag is sleeved on the discharge port, an operating glove is arranged inside the isolation cabin, one side of the isolation cabin 2 is communicated with the transfer cabin 1, and an equipment door is arranged on the other side of the isolation cabin;
one side of the transfer cabin 1 is communicated with the isolation cabin 2, the other side of the transfer cabin 1 is provided with a first airtight door, one side of the transfer cabin 1 communicated with the isolation cabin 2 is provided with a second airtight door, and the second airtight door is communicated with a sample inactivation module of a pathogen nucleic acid automatic processing and detecting unit;
the ventilation and filtration system comprises an isolation cabin ventilation and filtration device (comprising an air inlet 6 and an air outlet 7 shown in figure 1) and a delivery cabin ventilation HEPA filter 8; the isolation cabin ventilation filtering device is arranged at the top of the isolation cabin 2, and the transmission cabin ventilation HEPA filter 8 is arranged on a window body of the transmission cabin 1.
The communication mode of the sterilizer 11, the isolation cabin 2 and the transfer cabin 1 is as shown in fig. 3 (the arrow direction in the figure represents the gas flow direction, the inlet is air supply, the outlet is air exhaust, and the air supply and exhaust fans, the airtight valve and the filter are the same and are different in position), in some embodiments of the present invention, the transfer cabin ventilation HEPA filter 8 comprises an air supply high efficiency filter (filter 25), an air exhaust high efficiency filter (filter 25) and an exhaust fan (fan 24); a first airtight valve is arranged at an air inlet at the front end of the air supply high-efficiency filter, and a second airtight valve is arranged between the air exhaust high-efficiency filter and the exhaust fan; the air supply high-efficiency filter is arranged on the window body of the transfer cabin, and the air exhaust high-efficiency filter is arranged on the window body of the transfer cabin.
As shown in fig. 3 (the arrow direction in the figure represents the gas flow direction, the entering place is the air supply, the outflow is the air exhaust, the air supply is the same as the air exhaust fan, the airtight valve and the filter, and the positions are different), in some embodiments of the present invention, the ventilation and filtration device for the isolation cabin comprises: an air supply high-efficiency filter (filter 25), an air exhaust high-efficiency filter (filter 25), an air supply machine and an exhaust fan (fan 24); the air supply high-efficiency filter is arranged at the top of the isolation cabin 2, and the air exhaust high-efficiency filter is arranged at the top of the isolation cabin 2; the air exhaust fan is characterized in that a first closed valve (closed valve 26) is arranged between the air feeder and the air feeding high-efficiency filter, and a second closed valve (closed valve 26) is arranged between the exhaust fan and the air exhausting high-efficiency filter. The controller 22 is a controller, the differential pressure sensor 23 is a differential pressure sensor, the differential pressure sensor 23 monitors the differential pressure in the isolation cabin in real time and transmits a differential pressure signal to the controller 22, and the controller 22 adjusts the exhaust fan 24 according to an adjustment rule, so that the adjustment of the differential pressure in the isolation cabin is realized.
The utility model discloses an automatic operation mode of detection system is kept apart to unmanned high-risk pathogenic microorganism can improve work efficiency by a wide margin, reduces the requirement to operating personnel professional skill, is favorable to handling and detects the standardization and the objectification of flow, reduces the influence of operating personnel subjective factor to the result. But high-grade biological protection isolation design furthest reduces the leakage risk and the operating personnel's of high-risk sample exposure, makes the utility model discloses become one set of mobilizable high-risk pathogenic microorganism that can independently move and handle and detect the laboratory, be particularly suitable for lacking the mobile application occasion of high-grade biological laboratory facility, but the mobile processing and the prevention and control ability of the dangerous pathogen of real promotion.
In some embodiments of the present invention, the control system comprises an isolation chamber automatic control device and a pathogen nucleic acid automatic processing and detecting unit control device.
In some embodiments of the present invention, the isolation chamber automatic control device is disposed on the upper portion of the isolation chamber, and the pathogen nucleic acid automatic processing and detecting unit control device is disposed on the upper portion of the transfer chamber.
In some embodiments of the present invention, the material of the cabin body of the isolation cabin is a flexible transparent film.
In some embodiments of the present invention, the equipment door has an airtight zipper structure.
In some embodiments of the present invention, the pathogen nucleic acid automatic processing and detecting unit includes a sample inactivation module, a nucleic acid extraction module, and a nucleic acid detection module.
In some embodiments of the present invention, the high risk pathogenic microorganism unmanned isolation detection system comprises a display device for displaying the processing and detection results.
The embodiment of the second aspect of the utility model provides an automatic processing and detection method of high-risk pathogenic microorganism, adopt foretell any kind of device to accomplish, include the following step:
putting a reagent required by detection into a corresponding position of the isolation cabin through the transfer cabin;
the original sample is sent into a transfer cabin, and then sent into a pathogen nucleic acid automatic processing and detecting unit of an isolation cabin, and the pathogen nucleic acid automatic processing and detecting unit detects the sample;
and after the sample detection is finished, carrying out gas fumigation on the transfer cabin and the isolation cabin.
The device system mainly comprises an isolation cabin 2, a transmission cabin 1, a pathogen nucleic acid automatic processing and detection unit 15, a gas disinfection unit, a ventilation and filtration device, a master control unit, a human-computer interaction unit, a support frame 3 and the like.
The isolation cabin is provided with a plurality of operating gloves 14, one side of each operating glove is provided with an equipment door, the other side of each operating glove is connected with the transmission cabin, the pathogen nucleic acid automatic treatment and detection unit 15 is arranged in the isolation cabin 2, the top of the isolation cabin 2 is provided with an isolation cabin control screen 4, the isolation cabin control screen 4 is provided with a switch 16, the bottom of the isolation cabin is provided with a waste discharge port, the waste discharge port is connected with a transmission sleeve 9, and the transmission sleeve 9 is hermetically sleeved with a waste collection bag 10. The isolation cabin automatic control system consists of a display screen and a switch button.
A plurality of operating gloves 14 are arranged on the transparent window of the transfer chamber 1. One side of the transmission cabin 1 communicated with the outside is provided with a sealed door, one side of the transmission cabin 1 connected with the isolation cabin 2 is provided with the sealed door, the window body of the transmission cabin is provided with a ventilation HEPA filter 8, and one side of the transmission cabin is provided with a transparent window. The inner side closed door of the transfer chamber 1 is communicated with a sample inactivation module of the pathogen nucleic acid automatic processing and detecting unit in the isolation chamber 2. The top of the transmission cabin is provided with a pathogen nucleic acid automatic processing and detecting unit control screen 5.
With reference to fig. 4, a local laminar flow air outlet 131 is provided at one side of the bottom of the isolation cabin 2 for providing clean air protection for sample reservation of nucleic acid samples, and a high efficiency filter 132, a sealing valve 133 and a blower 134 are sequentially installed at the front end of the laminar flow air outlet 131.
The ventilation and filtration system of the isolation cabin consists of an air supply efficient filter, an air exhaust efficient filter, a sealing valve, an air supply machine and an exhaust fan. And a sealing valve is arranged between the fan and the filter.
The ventilation and filtration device of the transfer cabin consists of an air supply efficient filter, an air exhaust efficient filter, a closed valve and an exhaust fan. The air inlet at the front end of the air supply high-efficiency filter is provided with a sealing valve, and the sealing valve is arranged between the air exhaust high-efficiency filter and the exhaust fan.
With reference to fig. 2, the automatic pathogen nucleic acid processing and detecting unit 15 is horizontally installed in the isolation chamber 2, and mainly comprises three modules of sample inactivation 151, nucleic acid extraction 152, nucleic acid detection 153, and a nucleic acid sample reserving region 154. The sample inactivation module 152 is located on the left side of the unit and the sample first enters the module via the transfer chamber 2 and is inactivated by means of air bath heating. The target temperature and time of heating may be set by a program. During the sample heating process, the sample frame is oscillated horizontally to improve the heating uniformity and prevent the blood sample from coagulating. After the inactivation process is finished, the closed door on one side of the inactivation module 151 close to the nucleic acid extraction module 152 is automatically opened, and the mechanical arm group responsible for sample suction of the nucleic acid extraction module 152 opens the sample tube to quantitatively suck the sample and move the sample to a specific position of the extraction working area. And mixing the sample and the related extraction reagent according to a preset extraction program by the mechanical arm group responsible for extraction, and performing cracking, adsorption, cleaning, elution and other steps to obtain the sample nucleic acid extracting solution. The mechanical arm group in charge of sample retention firstly moves all the nucleic acid extracting solution into the sample retention tube, if the program comprises a detection step, the nucleic acid extracting solution is quantitatively absorbed and added into a detection card with the nucleic acid detection module 153 pre-sealed with related reagents, the detection card reacts according to a preset detection program, and the detection result is displayed on a human-computer interaction interface in real time. The remaining nucleic acid extract in the sample retention tube is manually closed by an operating glove and taken out.
The gas (steam) disinfection system consists of a disinfector, a disinfection pipeline, a sealed valve and the like, and can respectively carry out gas fumigation disinfection on the isolation cabin and the transfer cabin (see figure 3). The air supply pipeline of the gas (steam) disinfection system is respectively connected between the airtight valve in the air supply system of the isolation cabin and the transmission cabin and the air supply high-efficiency filter, and the airtight valve is respectively arranged between the air supply pipeline of the gas (steam) disinfection system and the air supply system of the isolation cabin and the transmission cabin. The air suction pipeline of the air (steam) disinfection system is respectively connected between the airtight valve in the isolation cabin and the air exhaust system of the transfer cabin and the high-efficiency air exhaust filter, and the airtight valve is respectively arranged between the air suction pipeline of the air (steam) disinfection system and the isolation cabin and the air exhaust system of the transfer cabin.
The procedure for sterilizing the isolation chamber is as follows: the method comprises the steps of firstly closing two airtight valves of an air supply and exhaust system and an air supply and exhaust machine of an isolation cabin, opening two airtight valves between a disinfection machine and the air supply and exhaust system of the isolation cabin, then starting the disinfection machine to generate disinfection gas, sequentially penetrating through an air supply efficient filter, entering the isolation cabin, penetrating through an air exhaust efficient filter, entering the disinfection machine through a disinfection pipeline, and continuously operating until a disinfection effect is achieved.
The procedure for sterilizing the transfer chamber is as follows: the method comprises the steps of firstly closing two airtight valves and an exhaust fan of an air supply and exhaust system of a transfer cabin, opening two airtight valves between a sterilizing machine and the air supply and exhaust system of the transfer cabin, then starting the sterilizing machine to generate sterilizing gas, sequentially penetrating through an air supply efficient filter, entering the transfer cabin, penetrating through an air exhaust efficient filter, entering the sterilizing machine through a sterilizing pipeline, and continuously operating until a sterilizing effect is achieved. The disinfectant gas (vapor) can be formaldehyde, chlorine dioxide, hydrogen peroxide, etc.
And (3) detection flow:
the detection process is specifically explained by taking the blood sample detection of the Ebola virus as an example.
1) And (5) preparing the work. Relevant reagents (a multi-hole plate for pre-sealing a magnetic bead method nucleic acid extraction reagent and a micro-fluidic detection chip for pre-sealing a nucleic acid detection reagent) and consumables (a liquid-transfer suction head, a sample retention centrifuge tube and the like) required by the system work are put into corresponding positions of the isolation cabin through the transfer cabin.
2) Sample connection/sample separation. The sample transport canister or blood collection tube (centrifuge tube) containing the original ebola blood sample is transferred from the left hatch door to the transfer hatch, and the hatch door is closed. The transport tank is opened in the transfer cabin through the operating handle cover to operating personnel, carries out the partial shipment or directly sends into the sample inactivation module of isolation capsule with heparin tube (centrifuging tube) via the sealing door on transfer cabin right side to the sample. The dispensed or retained sample can be returned to the transport tank.
3) Sample inactivation (optional). After a blood collection tube (centrifugal tube) filled with a blood sample enters the inactivation module, the inactivation module is vertically placed on a sample support, and a sealing door on the left side of the inactivation module is closed. Air bath inactivation was performed according to the set parameters (60 ℃, 30 min). In the inactivation process, the sample support drives the blood collection tube (centrifuge tube) to horizontally oscillate so as to promote the uniform temperature rise of the blood sample in the tube and prevent the blood sample from coagulating. And after inactivation is finished, the sample support stops oscillating, and the sealing cover at the sample outlet is automatically opened.
4) And (4) extracting nucleic acid. According to predetermineeing the procedure of drawing, press from both sides and get the arm and snatch heparin tube (centrifuging tube) to uncapping the platform on the sample support, open the tube cap, move the application of sample chamber that liquid arm absorbs 200 ~ 1000 mul blood sample to nucleic acid extraction perforated plate. The pick-up arm covers the tube lid back and places the blood collection tube (centrifuge tube) back into the sample holder. If there are a plurality of blood collection tubes (centrifuge tubes) on the sample holder, the above sample application steps are sequentially performed. After all samples are loaded, the pipetting mechanical arm respectively sucks related reagents, magnetic beads and blood samples from a plurality of reagent holes of the nucleic acid extraction multi-hole plate, and the steps of cracking, adsorption, cleaning, elution and the like are sequentially carried out under the coordination of the pipetting head and an external magnetic field, so that about 200-300 mu l of sample nucleic acid extracting solution is obtained.
5) And (3) detecting nucleic acid. And (3) sucking 40-80 mul of nucleic acid extracting solution by a liquid transferring mechanical arm, and adding the nucleic acid extracting solution into the sample adding hole of the detection chip. The nucleic acid extracting solution enters a reaction chamber of the chip and is mixed with the pre-sealed reagent to construct and complete a real-time fluorescence detection reaction system. And starting real-time fluorescence amplification according to a preset amplification program. And displaying the fluorescence detection result of the reaction chamber on a human-computer interaction interface in real time. The pipetting mechanical arm sucks all the residual nucleic acid extracting solution in the nucleic acid extracting multi-well plate into the sample reserving centrifugal tube to be used as a reserved sample. Before sucking the nucleic acid extracting solution to the nucleic acid sample reserving centrifuge tube, opening a laminar flow air outlet on one side of the bottom in the isolation cabin to provide clean air flowing in a single direction for a nucleic acid filling area, and protecting the air in the nucleic acid sample reserving centrifuge tube from being polluted by air in the cabin in the sample reserving process. After the pipetting mechanical arm moves the nucleic acid extracting solution into the sample reserving centrifuge tube, an operator manually covers the cover of the sample reserving tube through an operating glove. This laminar flow of air may be turned off after completion.
6) And (5) cleaning the waste. After the detection is finished, the operator throws all the used waste such as the nucleic acid extraction multi-plate, the detection chip, the pipette tip and the like in the isolation chamber into the waste collection bag through the waste transfer sleeve 9 by operating the gloves, and the waste is collected and then is treated separately.
7) Fumigating and sterilizing. And after the detection of each batch of samples is finished, carrying out thorough gas fumigation on the transfer chamber and the isolation chamber.
8) And (5) sample retention. After sterilization is completed, the transport tanks and unused blood collection tubes (centrifuge tubes) and the like in the transfer chamber can be taken out from the sample inlet. The nucleic acid sample retention tube in the isolation cabin can enter the transfer cabin through the sample inactivation module and then be taken out from the sample inlet.
The steps 1), 2), 6) and 8) need to be finished manually, and the core processing and detecting links from 3) to 5) are finished automatically by the system. 7) The starting is manually carried out by one key, and the starting is automatically carried out later.
It should be noted that the above embodiments can be freely combined as necessary. The above description is only for the preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. An unmanned isolation detection system for high-risk pathogenic microorganisms is characterized by comprising: the device comprises an isolation cabin, a transfer cabin, a pathogen nucleic acid automatic processing and detecting unit, a ventilation and filtration system and a control system;
the pathogen nucleic acid automatic processing and detecting unit is arranged in the isolation cabin, a waste discharge port is arranged at the bottom of the isolation cabin, a waste collecting bag is sleeved at the discharge port, an operating glove is arranged in the isolation cabin, one side of the isolation cabin is communicated with the transfer cabin, and an equipment door is arranged at the other side of the isolation cabin;
one side of the transfer cabin is communicated with the isolation cabin, the other side of the transfer cabin is provided with a first airtight door, one side of the transfer cabin, which is communicated with the isolation cabin, is provided with a second airtight door, and the second airtight door is communicated with a sample inactivation module of the pathogen nucleic acid automatic processing and detecting unit;
the ventilation and filtration system comprises an isolation cabin ventilation and filtration device and a transmission cabin ventilation and filtration device; the isolation cabin ventilation and filtration device is arranged at the top of the isolation cabin, and the transfer cabin ventilation and filtration device is arranged on a window body of the transfer cabin.
2. The unmanned isolation and detection system for high risk pathogenic microorganisms according to claim 1, wherein the ventilation and filtration device of the transfer cabin comprises an air supply efficient filter, an air exhaust efficient filter and an exhaust fan; a first airtight valve is arranged at an air inlet at the front end of the air supply high-efficiency filter, and a second airtight valve is arranged between the air exhaust high-efficiency filter and the exhaust fan; the air supply high-efficiency filter is arranged on the window body of the transfer cabin, and the air exhaust high-efficiency filter is arranged on the window body of the transfer cabin.
3. The unmanned detection system for high risk pathogenic microorganisms according to claim 1, wherein the ventilation and filtration device of the isolation cabin comprises: the air supply high-efficiency filter, the air exhaust high-efficiency filter, the air feeder and the exhaust fan; the air supply high-efficiency filter is arranged at the top of the isolation cabin, and the air exhaust high-efficiency filter is arranged at the top of the isolation cabin; the air supply device is characterized in that a first airtight valve is arranged between the air supply device and the air supply high-efficiency filter, and a second airtight valve is arranged between the exhaust fan and the air exhaust high-efficiency filter.
4. The system for unmanned detection and isolation of highly pathogenic microorganisms according to claim 1, wherein the control system comprises an isolation cabin automatic control device and a pathogen nucleic acid automatic processing and detection unit control device.
5. The system for unmanned detection and isolation of highly pathogenic microorganisms according to claim 4, wherein the automatic control device of the isolation chamber is arranged at the upper part of the isolation chamber, and the automatic control device of the pathogen nucleic acid processing and detecting unit is arranged at the upper part of the delivery chamber.
6. The unmanned detection system for high risk pathogenic microorganisms according to claim 1, wherein the cabin body of the isolation cabin is made of a flexible transparent film.
7. The unmanned detection system for high risk pathogenic microorganisms according to claim 1, wherein the equipment door has an airtight zipper structure.
8. The system for detecting the unmanned isolation of the highly pathogenic microorganisms according to claim 1, wherein the automated pathogen nucleic acid processing and detecting unit comprises a sample inactivation module, a nucleic acid extraction module, and a nucleic acid detection module.
9. The system for detecting the unmanned isolation of high-risk pathogenic microorganisms according to claim 1, wherein the system for detecting the unmanned isolation of high-risk pathogenic microorganisms comprises a display device for displaying processing and detection results.
10. The unmanned detection system that keeps apart of high dangerous pathogenic microorganism of claim 1, characterized in that, the isolation under-deck bottom one side be equipped with laminar flow air outlet and be used for providing clean air protection for nucleic acid sample stays the appearance, laminar flow air outlet front end installs high efficiency filter, airtight valve and fan in proper order.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201922180375.1U CN211620500U (en) | 2019-12-06 | 2019-12-06 | Unmanned isolation detection system for high-risk pathogenic microorganisms |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201922180375.1U CN211620500U (en) | 2019-12-06 | 2019-12-06 | Unmanned isolation detection system for high-risk pathogenic microorganisms |
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| CN211620500U true CN211620500U (en) | 2020-10-02 |
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