CN114480074A - PCR amplification operation cabinet and use method thereof - Google Patents
PCR amplification operation cabinet and use method thereof Download PDFInfo
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- CN114480074A CN114480074A CN202210383245.1A CN202210383245A CN114480074A CN 114480074 A CN114480074 A CN 114480074A CN 202210383245 A CN202210383245 A CN 202210383245A CN 114480074 A CN114480074 A CN 114480074A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
- B01D46/0027—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with additional separating or treating functions
- B01D46/0028—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with additional separating or treating functions provided with antibacterial or antifungal means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
- B01L1/04—Dust-free rooms or enclosures
Abstract
The invention relates to a PCR amplification operation cabinet and a using method thereof. The specimen preparation system comprises a specimen preparation module and a PCR amplification module, wherein the specimen preparation module comprises a specimen preparation room, a bottom air cavity, a side air cavity and a first top air cavity, a specimen preparation operation window is arranged in the specimen preparation room, a first air inlet and a specimen preparation area are arranged in the specimen preparation room, a first air draft device and a first disinfection and filtration device are arranged in the first top air cavity, an upper air duct air outlet communicated with the outside of an operation cabinet is arranged on the upper surface of the first top air cavity, and a lower air duct air outlet communicated with the inside of the specimen preparation room is arranged on the lower surface of the first top air cavity. Compared with the prior art, the invention ensures that the clean gas is filled in the whole sample preparation room, thereby ensuring that the sample and the reagent are not polluted and further preventing the cross contamination among the samples in the sample preparation room.
Description
Technical Field
The invention belongs to the technical field of biological sample treatment and detection, and particularly relates to a PCR amplification operation cabinet and a use method thereof.
Background
PCR nucleic acid detection is the main means for judging whether human body is infected with virus. The PCR nucleic acid detection is to find out whether the nucleic acid of the virus invaded from the outside exists in the respiratory tract specimen, blood or feces of the patient to determine whether the patient is infected by the virus. Thus, once a patient is detected as "positive" for a nucleic acid, the presence of the virus in the patient is verified. However, since the safety and no pollution need to be ensured when PCR nucleic acid detection is performed, high requirements are put on the operating environment, and the existing PCR nucleic acid detection is mostly performed in a PCR laboratory.
Existing PCR laboratories are generally divided into four separate working areas: reagent preparation district, sample preparation district, amplification district and product analysis district, in order to avoid cross contamination, operating personnel must follow single direction strictly to go on when entering each work area, and the environmental field requires highly, and complex operation has complicated, has restricted PCR nucleic acid detection's wide application. In order to solve the above problems, patent application document CN202020725842.4 discloses a biosafety cabinet for PCR experiments. However, the solution of the patent document is inconvenient for the operator to operate and there is a risk of cross-contamination between the samples.
Therefore, a technical solution that can be easily operated by a worker and avoid cross contamination among a plurality of samples is needed.
Disclosure of Invention
One of the objects of the present invention is: aiming at the defects of the prior art, the invention provides a PCR amplification operation cabinet, which aims to solve the problems that the operation of workers is inconvenient and cross contamination risks exist among a plurality of samples in the existing PCR nucleic acid detection.
In order to achieve the purpose, the invention adopts the following technical scheme:
a PCR amplification cabinet comprising:
the specimen preparation module comprises a specimen preparation room, a bottom air cavity arranged below the specimen preparation room, a lateral air cavity arranged at the side and/or the rear of the specimen preparation room, and a first top air cavity arranged above the specimen preparation room, wherein a specimen preparation operation window is arranged on the front of the specimen preparation room, a first air inlet and a specimen preparation area are arranged on the inner bottom surface of the specimen preparation room, the first air inlet is positioned in front of the specimen preparation area, the width of the first air inlet corresponds to that of the specimen preparation operation window, the inside of the specimen preparation room is communicated with the bottom air cavity through the first air inlet, the bottom air cavity is communicated with the first top air cavity through the lateral air cavity, and the first top air cavity is internally provided with a first air draft device and a first disinfection and filtration device, an upper air channel air outlet communicated with the outside of the operation cabinet is arranged on the upper surface of the first top air cavity, and a lower air channel air outlet communicated with the inside of the specimen preparation room is arranged on the lower surface of the first top air cavity;
a PCR amplification module located on one side of the specimen preparation module.
The PCR amplification operation cabinet further comprises an image identification module, wherein the image identification module comprises an image acquisition device, an image detection module and an alarm device, the image acquisition device and the alarm device are arranged in the specimen preparation room, the image acquisition device takes the direction facing the specimen preparation operation window as the image acquisition direction, the image acquisition device is used for acquiring image information, and the image detection module is used for detecting and identifying whether the image information meets the requirement of wearing a mask.
As a preferred scheme of the PCR amplification operation cabinet, a side air inlet is formed in the position, close to the bottom surface of the interior of the specimen preparation room, of the side air cavity, the interior of the specimen preparation room is communicated with the side air cavity through the side air inlet, and the width of the side air inlet corresponds to the length of the side length of the corresponding specimen preparation area.
As a preferred embodiment of the PCR amplification operation cabinet, a sample preparation operation window door corresponding to the sample preparation operation window is arranged on the front surface of the sample preparation room, a window and door accommodating room is arranged in front of the first top air cavity, a window and door through groove corresponding to the specimen preparation operation window is arranged on the bottom surface of the window and door accommodating room, the inner side of the specimen preparation operation window is provided with a window and door track which extends upwards to the interior of the window and door accommodating room, the specimen preparation operation window door is arranged at the specimen preparation operation window along the window door track in a sliding manner, the window and door accommodating room is internally provided with a lifting motor and a lifting conveyor belt, the lifting motor is in transmission connection with the specimen preparation operation window and door through the lifting conveyor belt, the lifting motor can drive the specimen preparation operation window door to reciprocate along the window door track through the lifting conveyor belt.
As a preferred scheme of the PCR amplification operation cabinet, the first top air cavity is internally provided with an inner air duct, the first air draft device comprises a first air draft air inlet and a first air draft air outlet, the first top air cavity is communicated with the first air draft device through the first air draft air inlet, the side surface of the inner air duct is provided with an air duct air inlet communicated with the first air draft air outlet, the upper surface of the inner air duct is communicated with the air outlet of the upper air duct, the lower surface of the inner air duct is communicated with the air outlet of the lower air duct, and the first disinfection filtering device is arranged inside the inner air duct.
As a preferred scheme of the PCR amplification operation cabinet, the first disinfection and filtration device is arranged at the air outlet of the upper air duct and/or the air outlet of the lower air duct.
As a preferred scheme of the PCR amplification operation cabinet, the PCR amplification module comprises a PCR amplification room and a second top air cavity arranged above the PCR amplification room, a PCR amplification operation window and a PCR amplification operation window door correspondingly arranged with the PCR amplification operation window are arranged on the front side of the PCR amplification room, a second air inlet is arranged on the side surface of the PCR amplification room, the outside of the operation cabinet is communicated with the inside of the PCR amplification room through the second air inlet, a second disinfection and filtration device is arranged in the second air inlet, a lower air inlet communicated with the inside of the PCR amplification room is arranged below the second top air cavity, a second air draft device and a third disinfection and filtration device are arranged in the second top air cavity, the second air draft device comprises a second air draft air inlet and a second air draft air outlet, and the second top air draft cavity is communicated with the second air draft device through the second air draft air inlet, and the second air exhaust device is communicated with the outside of the operation cabinet through the second air exhaust outlet.
In a preferred embodiment of the cabinet for PCR amplification according to the present invention, a storage plate for placing a PCR instrument is disposed inside the PCR amplification chamber.
As a preferred embodiment of the PCR amplification operation cabinet of the present invention, the PCR amplification operation cabinet further includes a movable frame body, the movable frame body is used for supporting the specimen preparation module and the PCR amplification module, the movable frame body is disposed below the specimen preparation module and the PCR amplification module, and a movable roller is disposed below the movable frame body.
One of the purposes of the invention has the following beneficial effects:
1) under the action of the first air draft device, gas inside the sample preparation room and/or gas at the position of the sample preparation operation window sequentially pass through the first air inlet, the bottom air cavity, the side air cavity, the first top air cavity and the first air draft device; a part of gas is discharged to the interior of the specimen preparation room through the air outlet of the lower air duct, and meanwhile, the gas can be disinfected and sterilized through the first disinfection and filtration device in the first top air cavity, so that clean gas is discharged from the air outlet of the lower air duct and fills the whole specimen preparation room, a sample and a reagent are not polluted, and cross contamination among samples in the specimen preparation room is prevented; the other part of gas is discharged to the outside of the operation cabinet through the air outlet of the upper air duct, and because the air inlet volume of the first air inlet is greater than the air outlet volume of the air outlet of the lower air duct, negative pressure is formed inside the specimen preparation room, so that the gas in the specimen preparation room is prevented from being directly discharged from the specimen preparation operation window, and the specimens are prevented from being infected to workers through air; moreover, when the worker prepares the specimen, the specimen preparation operation window is not blocked by the isolation glass, so that the worker can conveniently operate the specimen preparation operation window;
2) according to the invention, the first air inlet is positioned in front of the specimen preparation area, the width of the first air inlet corresponds to that of the specimen preparation operation window, and when air outside the operation cabinet enters the specimen preparation room from the specimen preparation operation window, the air outside the operation cabinet flows to the first air inlet due to the fact that the first air inlet is in a negative pressure state and the width of the first air inlet corresponds to that of the specimen preparation operation window, so that the air outside the operation cabinet is prevented from contacting and polluting samples and reagents in the specimen preparation area; meanwhile, when the internal gas in the sample preparation room is discharged towards the direction of the sample preparation operation window, the internal gas in the sample preparation room can flow to the first air inlet due to the fact that the first air inlet is in a negative pressure state, and therefore the internal gas in the sample preparation room is prevented from contacting with workers.
The second purpose of the invention is: aiming at the defects of the prior art, the invention provides a method for using a PCR amplification operation cabinet.
In order to achieve the purpose, the invention adopts the following technical scheme:
the method for using the PCR amplification operation cabinet, which uses the PCR amplification operation cabinet in one of the above objects, comprises the following steps:
s100, starting a first air draft device and an image recognition module, and preparing a sample in a sample preparation room; when a sample is prepared, the image acquisition device acquires image information of the face of a worker in real time and transmits the image information to the image detection module; the image detection module detects and identifies the image information of the face of the person and judges whether the image information of the face of the person meets the requirement of wearing a mask or not; if not, starting an alarm device;
s200, after sample preparation is completed, sealing the sample and moving the sample to a PCR amplification module;
s300, performing PCR amplification on the sample at the PCR amplification module.
The second object of the invention has the following beneficial effects:
1) under the action of the first air draft device, gas inside the specimen preparation room and/or gas at the opening of the specimen preparation operation window sequentially pass through the first air inlet, the bottom air cavity, the side air cavity, the first top air cavity and the first air draft device; a part of gas is discharged to the interior of the specimen preparation room through the air outlet of the lower air duct, and meanwhile, the gas can be disinfected and sterilized through the first disinfection and filtration device in the first top air cavity, so that clean gas is discharged from the air outlet of the lower air duct and fills the whole specimen preparation room, a sample and a reagent are not polluted, and cross contamination among samples in the specimen preparation room is prevented; the other part of gas is discharged to the outside of the operation cabinet through the air outlet of the upper air duct, and because the air inlet volume of the first air inlet is greater than the air outlet volume of the air outlet of the lower air duct, negative pressure is formed inside the specimen preparation room, so that the gas in the specimen preparation room is prevented from being directly discharged from the specimen preparation operation window, and the specimens are prevented from being infected to workers through air; moreover, when the worker prepares the specimen, the specimen preparation operation window is not blocked by the isolation glass, so that the worker can conveniently operate the specimen preparation operation window;
2) according to the invention, the first air inlet is positioned in front of the specimen preparation area, the width of the first air inlet corresponds to that of the specimen preparation operation window, and when air outside the operation cabinet enters the specimen preparation room from the specimen preparation operation window, the air outside the operation cabinet flows to the first air inlet due to the fact that the first air inlet is in a negative pressure state and the width of the first air inlet corresponds to that of the specimen preparation operation window, so that the air outside the operation cabinet is prevented from contacting and polluting samples and reagents in the specimen preparation area; meanwhile, when the internal gas in the sample preparation room is discharged towards the direction of the sample preparation operation window, the internal gas in the sample preparation room can flow to the first air inlet due to the fact that the first air inlet is in a negative pressure state, and therefore the internal gas in the sample preparation room is prevented from contacting with workers.
Drawings
Fig. 1 is a schematic structural diagram of an operation cabinet of the present invention.
Fig. 2 is a front view of the operation cabinet of the present invention.
Fig. 3 is a front view of the operation cabinet of the present invention, with a partial structure being cut away.
Fig. 4 is a schematic diagram of the gas flow based on fig. 3.
Fig. 5 is a sectional view taken along the line a-a in fig. 2.
Fig. 6 is a schematic diagram of the gas flow based on fig. 5.
Fig. 7 is a sectional view taken in the direction B-B in fig. 2.
Fig. 8 is a schematic diagram of the gas flow based on fig. 7.
In the figure:
1-a specimen preparation module; 11-a specimen preparation room; 111-a first air inlet; 112-specimen preparation operating window; 113-specimen preparation operating window and door; 114-a specimen preparation area; 12-bottom air chamber; 13-lateral air chambers; 131-side air inlet; 14-a first top air chamber; 141-a first air extraction device; 142-an inner air duct; 1421 — air duct intake; 1422-upper duct outlet; 1423-lower duct outlet; 1424 — first sterilizing filter device; 15-window and door receiving room; 151-a lifting motor;
2-PCR amplification module; 21-PCR amplification chamber; 211-a second air inlet; 2111-a second sterilizing and filtering device; 212-PCR amplification window of operation; 213-PCR amplification operation window; 214-a shelf; 22-a second top air cavity; 221-a second air extraction device; 2211-second air exhaust outlet; 222-a third sterile filtration device; 223-lower air inlet;
3, moving the frame body; 31-moving the roller;
4-a power socket;
5-ultraviolet disinfection lamp;
6-control panel;
7-an image acquisition device;
8-alarm device.
Detailed Description
In order to make the technical solutions and advantages of the present invention clearer, the present invention and its advantages will be described in further detail below with reference to the following detailed description and the accompanying drawings, but the embodiments of the present invention are not limited thereto.
Example 1
As shown in fig. 1 to 8, the PCR amplification operation cabinet includes:
the specimen preparation module 1, the specimen preparation module 1 includes a specimen preparation room 11, a bottom air cavity 12 arranged below the specimen preparation room 11, a side air cavity 13 arranged at the side and/or the rear of the specimen preparation room 11, and a first top air cavity 14 arranged above the specimen preparation room 11, the specimen preparation room 11 is provided with a specimen preparation operation window 112 and a control panel 6 at the front, the specimen preparation room 11 is provided with a first air inlet 111 and a specimen preparation area 114 on the inner bottom surface, the first air inlet 111 is positioned in front of the specimen preparation area 114, the width of the first air inlet 111 corresponds to the width of the specimen preparation operation window 112, the inside of the specimen preparation room 11 is communicated with the bottom air cavity 12 through the first air inlet 111, the bottom air cavity 12 is communicated with the first top air cavity 14 through the side air cavity 13, the first top air cavity 14 is provided with a first air draft device 141 and a first disinfection and filtration device 1424 inside, an upper air duct air outlet 1422 communicated with the outside of the operation cabinet is arranged on the upper surface of the first top air cavity 14, and a lower air duct air outlet 1423 communicated with the inside of the specimen preparation room 11 is arranged on the lower surface of the first top air cavity 14;
and the PCR amplification module 2 is positioned at one side of the specimen preparation module 1.
The working principle of the embodiment is as follows:
1) as shown in fig. 4 and 6, the first air draft device 141 is started, and under the action of the first air draft device 141, the gas inside the specimen preparation room 11 and the gas at the specimen preparation operation window 112 sequentially pass through the first air inlet 111, the bottom air cavity 12, the side air cavity 13, the first top air cavity 14 and the first air draft device 141; a part of gas is discharged into the specimen preparation room 11 through the lower air duct outlet 1423, so that negative pressure is formed at the first air inlet 111, and meanwhile, the gas can be sterilized through the first sterilizing and filtering device 1424 in the first top air cavity 14, so that clean gas is discharged from the lower air duct outlet 1423 and fills the whole specimen preparation room 11, so that the samples and the reagents are not polluted, and cross contamination among the samples in the specimen preparation room 11 is prevented; the other part of the gas is discharged to the outside of the operation cabinet through the upper air duct outlet 1422, so that the air inlet volume of the first air inlet 111 is greater than the air outlet volume of the lower air duct outlet 1423, and negative pressure is formed inside the specimen preparation room 11; when the gas outside the operation cabinet enters the sample preparation room 11 from the sample preparation operation window 112, the gas outside the operation cabinet flows to the first air inlet 111 because the first air inlet 111 is in a negative pressure state, so that the gas outside the operation cabinet is prevented from contacting and polluting the sample and the reagent in the sample preparation area 114; meanwhile, when the internal gas of the sample preparation room 11 is discharged towards the direction of the sample preparation operation window 112, the internal gas of the sample preparation room 11 flows to the first air inlet 111 because the first air inlet 111 is in a negative pressure state, so that the internal gas of the sample preparation room 11 is prevented from contacting with workers;
2) subsequently, the worker prepares the sample for PCR amplification within the specimen preparation room 11; after the sample preparation is finished, sealing the sample and moving the sample to the PCR amplification module 2 at one side for PCR amplification;
3) after completion of PCR amplification, the sample is sealed and moved to the next process.
Preferably, the mask further comprises an image recognition module, the image recognition module comprises an image acquisition device 7, an image detection module and an alarm device 8, the image acquisition device 7 and the alarm device 8 are arranged inside the sample preparation room 11, the image acquisition device 7 takes the direction towards the sample preparation operation window 112 as the image acquisition direction, the image acquisition device 7 is used for acquiring image information, and the image detection module is used for detecting whether the image information meets the requirement of wearing the mask; specifically, the image acquisition device 7 is an electronic camera, the image detection module comprises a control processor and a memory, an image detection program is stored in the memory, the image detection program for detecting whether the face conforms to the requirement of wearing the mask is the prior art, and the alarm device 8 is an audible and visual alarm. The working principle of the image recognition module is as follows: the image acquisition device 7 acquires image information of the face of the worker in real time and transmits the image information to the image detection module; the image detection module detects image information of the face of the identified person and judges whether the image information of the face of the person meets the requirement of wearing the mask; if not, the alarm device 8 is started. Through the setting, when the staff operates 11 inside samples through sample preparation operation window 112 between the preparation of sample, image acquisition device 7 gathers the image information of staff's face in real time and transmits to the image detection module, when the image detection module detects the staff and discerns that the staff is not conform to and wears the gauze mask requirement, then sends the police dispatch newspaper through alarm device 8 to in time prevent the staff to continue the operation, and then avoid sample and staff to take place the contact.
Preferably, the side air cavity 13 is provided with a side air inlet 131 near the bottom surface of the interior of the specimen preparation room 11, the interior of the specimen preparation room 11 is communicated with the side air cavity 13 through the side air inlet 131, and the width of the side air inlet 131 corresponds to the length of the side length of the corresponding specimen preparation area 114. Through the above arrangement, gas in the specimen preparation area 114 can enter the lateral air cavity 13 through the lateral air inlet 131 in time, so that the cross contamination of samples and reagents in the specimen preparation area 114 is further avoided, the possibility of discharging the gas inside the specimen preparation room 11 towards the specimen preparation operation window 112 is reduced, and the contact between the gas inside the specimen preparation room 11 and workers is avoided.
Preferably, the specimen preparation room 11 is further provided with a specimen preparation operation window 113 on the front side, the specimen preparation operation window 113 is correspondingly arranged on the specimen preparation operation window 112, the first top air chamber 14 is provided with a window door accommodating room 15 in the front side, the window door accommodating room 15 is provided with a window door through groove corresponding to the specimen preparation operation window 113 on the bottom surface, the inner side of the specimen preparation operation window 112 is provided with a window door rail extending upwards to the inside of the window door accommodating room 15, the specimen preparation operation window 113 is arranged at the specimen preparation operation window 112 along the window door rail in a sliding manner, the window door accommodating room 15 is internally provided with a lifting motor 151 and a lifting conveyor belt, the lifting motor 151 is in transmission connection with the specimen preparation operation window 113 through the lifting conveyor belt, and the lifting motor 151 can drive the specimen preparation operation window 113 to reciprocate along the window door rail through the lifting conveyor belt. Through the arrangement, when the sample preparation module 1 is in a non-working state, the sample preparation room 11 can be sealed through the sample preparation operation window 113, so that the pollution caused by the fact that external gas of the operation cabinet enters the sample preparation room 11 is avoided; meanwhile, the specimen preparation operation window 113 can reciprocate up and down along the window track at the specimen preparation operation window 112.
More preferably, the image sensing module is in electrical communication with the first air extraction device 141 and the lift motor 151. Through the arrangement, when the image detection module detects and judges that the image information of the face of the worker does not meet the requirement of wearing the mask, if the specimen preparation operation window door 113 is not opened at the moment, the lifting motor 151 is locked and limited, so that the worker can not open the specimen preparation operation window door 113, and the worker is prevented from contacting the sample; if the specimen preparation operation window 113 is opened at this time, the air draft power of the first air draft device 141 is increased, so that the cleaning effect inside the specimen preparation room 11 is enhanced, and the contact probability between the worker and the sample is reduced.
Preferably, the first top air chamber 14 is further provided with an inner air duct 142 inside, the first air draft device 141 includes a first air draft air inlet and a first air draft air outlet, the first top air chamber 14 is communicated with the first air draft device 141 through the first air draft air inlet, the inner air duct 142 is provided with an air duct air inlet 1421 on the side surface, the inner air duct 142 is communicated with the upper air duct air outlet 1422 on the upper surface, the inner air duct 142 is communicated with the lower air duct air outlet 1423 on the lower surface, and the first disinfection filter 1424 is arranged inside the inner air duct 142. Through the above arrangement, the structure of the inner air duct 142 is favorable for the internal air flow of the first top air chamber 14, so as to improve the disinfection effect of air, and ensure that the air discharged from the upper air duct outlet 1422 and the lower air duct outlet 1423 is clean air.
Preferably, a first sterilizing filter 1424 is disposed at the upper duct outlet 1422 and/or the lower duct outlet 1423. Through the arrangement, the disinfection effect of gas is further improved, and clean gas is discharged from the upper air duct air outlet 1422 and the lower air duct air outlet 1423.
Preferably, the PCR amplification module 2 comprises a PCR amplification room 21 and a second top air cavity 22 arranged above the PCR amplification room 21, the PCR amplification room 21 is provided with a PCR amplification operation window 212 and a PCR amplification operation window door 213 correspondingly arranged with the PCR amplification operation window 212 on the front side, the PCR amplification room 21 is provided with a second air inlet 211 on the side surface, the outside of the operation cabinet is communicated with the inside of the PCR amplification room 21 through the second air inlet 211, the second air inlet 211 is internally provided with a second disinfection and filtration device 2111, the lower part of the second top air cavity 22 is provided with a lower air inlet 223 communicated with the inside of the PCR amplification room 21, the second top air cavity 22 is internally provided with a second air draft device 221 and a third disinfection and filtration device 222, the second air draft device 221 comprises a second air draft air inlet and a second air draft air outlet 2211, the second top air cavity 22 is communicated with the second air draft device 221 through the second air draft air inlet, the second air draft device 221 is communicated with the outside of the operation cabinet through a second air draft air outlet 2211. The working principle of the PCR amplification module 2 is as follows: as shown in fig. 4 and 8, the second air draft device 221 is started, and under the action of the second air draft device 221, the gas inside the PCR amplification chamber 21, the gas at the PCR amplification operation window 212 and the gas at the second air inlet 211 sequentially pass through the lower air inlet 223, the second top air cavity 22 and the second air draft device 221, and are discharged to the outside of the operation cabinet through the second air draft air outlet 2211, so that a negative pressure is formed on the top surface inside the PCR amplification chamber 21; meanwhile, the gas is sterilized and disinfected through the third sterilizing and filtering device 222 in the second top air chamber 22, and the gas passing through the second air inlet 211 is sterilized and disinfected through the second sterilizing and filtering device 2111 in the second air inlet 211; after the worker puts the sample into the PCR instrument in the PCR amplification room 21, the amplification time of the PCR instrument is set, and the PCR amplification operation window 213 is closed; at this time, under the action of the second air draft device 221, the air outside the operation cabinet sequentially passes through the second air inlet 211, the lower air inlet 223, the second top air cavity 22 and the second air draft device 221, and is discharged to the outside of the operation cabinet through the second air draft air outlet 2211; meanwhile, the gas is sterilized and disinfected by the second sterilizing and filtering device 2111 and the third sterilizing and filtering device 222 in sequence. Through the arrangement, under the action of the second air draft device 221, negative pressure is formed on the top surface inside the PCR amplification room 21, so that gas in the PCR amplification room 21 is prevented from being directly exhausted from the PCR amplification operation window 212, and the specimens are prevented from being infected to workers through air; meanwhile, the gas is sterilized by the third sterilizing and filtering device 222 in the second top air chamber 22, so that the discharged gas is clean; furthermore, the gas passing through the second air inlet 211 is sterilized by the second sterilizing and filtering device 2111 in the second air inlet 211, so as to ensure that the gas entering the PCR amplification chamber 21 during PCR amplification is clean gas.
Preferably, the PCR amplification chamber 21 is provided with a placing plate 214 for placing a PCR instrument therein. Through the arrangement, a plurality of PCR instruments can be placed in the PCR amplification chamber 21, and the working efficiency is improved.
Preferably, the kit further comprises a moving frame body 3, wherein the moving frame body 3 is used for supporting the specimen preparation module 1 and the PCR amplification module 2, the moving frame body 3 is arranged below the specimen preparation module 1 and the PCR amplification module 2, and the moving frame body 3 is provided with a moving roller 31 below. Through the arrangement, the whole operation cabinet can be moved by the worker through the movable frame body 3.
Preferably, the specimen preparation room 11 and/or the PCR amplification room 21 are provided with a power socket 4 inside. Through the arrangement, the sample preparation room 11 and the PCR amplification room 21 can be conveniently operated by the worker through the electrified instrument, so that the operation of the worker is further facilitated.
Preferably, the specimen preparation room 11 and/or the PCR amplification room 21 are internally provided with an ultraviolet sterilizing lamp 5. Through the setting, when the sample preparation module 1 or the PCR amplification chamber 21 is in a non-working state, the sterilization and disinfection of the internal environment can be carried out by opening the corresponding ultraviolet disinfection lamp 5, and then the sample cross contamination is avoided.
Example 2
The method for using the PCR amplification operating cabinet in the embodiment 1 comprises the following steps:
s100, starting the first air draft device 141 and the image recognition module, and preparing a sample in the sample preparation room 11; when a sample is prepared, the image acquisition device 7 acquires image information of the face of a worker in real time and transmits the image information to the image detection module; the image detection module detects image information for identifying the face of a person and judges whether the image information of the face of the person meets the requirement of wearing the mask; if not, starting the alarm device 8; the image recognition module is arranged in the embodiment, when a worker operates an internal sample in the sample preparation room through the sample preparation operation window, the image acquisition device acquires image information of the face of the worker in real time and transmits the image information to the image detection module, and when the image detection module detects and recognizes that the worker does not meet the mask wearing requirement, the alarm device gives an alarm, so that the worker is prevented from continuing to operate in time, and the sample is prevented from contacting the worker;
s200, after sample preparation is completed, sealing the sample and moving the sample to the interior of the PCR amplification chamber 21;
s300, starting the second air draft device 221, and performing PCR amplification on the sample in the PCR amplification room 21.
Variations and modifications to the above-described embodiments may also occur to those skilled in the art, which fall within the scope of the invention as disclosed and taught herein. Therefore, the present invention is not limited to the above-mentioned embodiments, and any obvious improvement, replacement or modification made by those skilled in the art based on the present invention is within the protection scope of the present invention. Furthermore, although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.
Claims (10)
- A PCR amplification operation cabinet, which is characterized by comprising:the specimen preparation module (1), the specimen preparation module (1) includes a specimen preparation room (11), a bottom air cavity (12) arranged below the specimen preparation room (11), a side air cavity (13) arranged on the side and/or the rear of the specimen preparation room (11), and a first top air cavity (14) arranged above the specimen preparation room (11), the specimen preparation room (11) is provided with a specimen preparation operation window (112) on the front surface, the specimen preparation room (11) is provided with a first air inlet (111) and a specimen preparation area (114) on the inner bottom surface, the first air inlet (111) is positioned in front of the specimen preparation area (114), the width of the first air inlet (111) corresponds to that of the specimen preparation operation window (112), the inside of the specimen preparation room (11) is communicated with the bottom air cavity (12) through the first air inlet (111), the bottom air cavity (12) is communicated with the first top air cavity (14) through the side air cavity (13), a first air draft device (141) and a first disinfection and filtration device (1424) are arranged in the first top air cavity (14), an upper air duct air outlet (1422) communicated with the outside of the operation cabinet is arranged on the first top air cavity (14), and a lower air duct air outlet (1423) communicated with the inside of the specimen preparation room (11) is arranged on the lower surface of the first top air cavity (14);a PCR amplification module (2), wherein the PCR amplification module (2) is positioned at one side of the specimen preparation module (1).
- 2. The PCR amplification cabinet according to claim 1, wherein: still include the image recognition module, the image recognition module includes image acquisition device (7), image detection module and alarm device (8), image acquisition device (7) with alarm device (8) set up the inside between the preparation of sample (11), image acquisition device (7) are towards the direction of sample preparation operation window (112) is the image acquisition direction, and image acquisition device (7) are used for gathering image information, the image detection module is used for detecting the discernment whether image information accords with the requirement of wearing the gauze mask.
- 3. The PCR amplification cabinet of claim 1 or 2, wherein: the side air cavity (13) is provided with a side air inlet (131) at a position close to the inner bottom surface of the specimen preparation room (11), the inner part of the specimen preparation room (11) is communicated with the side air cavity (13) through the side air inlet (131), and the width of the side air inlet (131) corresponds to the length of the side length of the corresponding specimen preparation area (114).
- 4. The PCR amplification cabinet of claim 1 or 2, wherein: the specimen preparation room (11) is also provided with a specimen preparation operation window door (113) which is correspondingly arranged with the specimen preparation operation window (112) on the front side, the first top air cavity (14) is provided with a window door accommodating room (15) in the front side, the bottom surface of the window door accommodating room (15) is provided with a window door through groove corresponding to the specimen preparation operation window door (113), the inner side of the specimen preparation operation window (112) is provided with a window door track which extends upwards to the inside of the window door accommodating room (15), the specimen preparation operation window door (113) is arranged at the specimen preparation operation window (112) along the window door track in a sliding manner, the window door accommodating room (15) is internally provided with a lifting motor (151) and a lifting conveyor belt, and the lifting motor (151) is in transmission connection with the specimen preparation operation window door (113) through the lifting conveyor belt, the lifting motor (151) can drive the specimen preparation operation window door (113) to reciprocate along the window door track through the lifting conveyor belt.
- 5. The PCR amplification cabinet of claim 1 or 2, wherein: first top wind chamber (14) still are provided with interior wind channel (142) in inside, first updraft ventilator (141) include first convulsions air intake and first convulsions air outlet, first top wind chamber (14) pass through first convulsions air intake with first updraft ventilator (141) intercommunication, interior wind channel (142) be provided with in the side with wind channel air intake (1421) of first convulsions air outlet intercommunication, interior wind channel (142) on the top with last wind channel air outlet (1422) intercommunication, interior wind channel (142) under with wind channel air outlet (1423) intercommunication down, first disinfection filter equipment (1424) set up inside wind channel (142).
- 6. The PCR amplification cabinet of claim 1 or 2, wherein: the first disinfection filtering device (1424) is arranged at the upper air duct air outlet (1422) and/or the lower air duct air outlet (1423).
- 7. The PCR amplification cabinet of claim 1 or 2, wherein: the PCR amplification module (2) comprises a PCR amplification room (21) and a second top air cavity (22) arranged above the PCR amplification room (21), wherein a PCR amplification operation window (212) and a PCR amplification operation window door (213) correspondingly arranged with the PCR amplification operation window (212) are arranged on the front side of the PCR amplification room (21), a second air inlet (211) is arranged on the side surface of the PCR amplification room (21), the outside of the operation cabinet is communicated with the inside of the PCR amplification room (21) through the second air inlet (211), a second disinfection and filtration device (2111) is arranged in the second air inlet (211), a lower air inlet (223) communicated with the inside of the PCR amplification room (21) is arranged below the second top air cavity (22), and a second air draft device (221) and a third disinfection and filtration device (222) are arranged in the second top air cavity (22), second updraft ventilator (221) includes second convulsions air intake and second convulsions air outlet (2211), second top wind chamber (22) passes through the second convulsions air intake with second updraft ventilator (221) intercommunication, second updraft ventilator (221) pass through second convulsions air outlet (2211) with the outside intercommunication of operation cabinet.
- 8. The PCR amplification cabinet of claim 7, wherein: the PCR amplification chamber (21) is internally provided with a storage plate (214) for placing a PCR instrument.
- 9. The PCR amplification cabinet of claim 1 or 2, wherein: still including removing support body (3), remove support body (3) and be used for supporting sample preparation module (1) with PCR amplification module (2), remove support body (3) and set up sample preparation module (1) with the below of PCR amplification module (2), it is provided with removal gyro wheel (31) in the below to remove support body (3).
- Use of a PCR amplification cabinet according to any of claims 2 to 9, comprising the steps of:s100, starting a first air draft device (141) and an image recognition module, and preparing a sample in a sample preparation room (11); when a sample is prepared, the image acquisition device (7) acquires image information of the face of a worker in real time and transmits the image information to the image detection module; the image detection module detects and identifies the image information of the face of the person and judges whether the image information of the face of the person meets the requirement of wearing a mask or not; if not, starting an alarm device (8);s200, after sample preparation is completed, sealing the sample and moving the sample to a PCR amplification module (2);s300, performing PCR amplification on the sample at the PCR amplification module (2).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210383245.1A CN114480074A (en) | 2022-04-13 | 2022-04-13 | PCR amplification operation cabinet and use method thereof |
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| CN202210383245.1A CN114480074A (en) | 2022-04-13 | 2022-04-13 | PCR amplification operation cabinet and use method thereof |
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| CN202210383245.1A Pending CN114480074A (en) | 2022-04-13 | 2022-04-13 | PCR amplification operation cabinet and use method thereof |
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| CN110331084A (en) * | 2019-08-06 | 2019-10-15 | 中国人民解放军军事科学院军事医学研究院 | Nucleic acid extraction instrument and detection system |
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