CN115505520A - Full-automatic nucleic acid sample processing system - Google Patents
Full-automatic nucleic acid sample processing system Download PDFInfo
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Abstract
The invention belongs to the technical field of nucleic acid detection, and particularly relates to a full-automatic nucleic acid sample processing system which comprises an outer rack, wherein a reagent loading and configuration temporary storage mechanism, a reagent transfer buffer mechanism, a sample cup separating processing mechanism, a nucleic acid extraction mechanism and a packaging mechanism are sequentially arranged in the outer rack, the reagent loading and configuration temporary storage mechanism is connected with the reagent transfer buffer mechanism, the reagent transfer buffer mechanism is connected with the cup separating processing mechanism, the sample cup separating processing mechanism is connected with the nucleic acid extraction mechanism, and the nucleic acid extraction mechanism is connected with the packaging mechanism. The invention integrates the intelligent and automatic functions, integrates the steps of nucleic acid extraction, purification, amplification detection, storage and the like into a whole and carries out automatically, has quick, efficient, safe and accurate detection, avoids cross contamination or nucleic acid contamination, saves a large amount of manpower and has low detection cost.
Description
Technical Field
The invention belongs to the technical field of nucleic acid detection, and particularly relates to a full-automatic nucleic acid sample processing system.
Background
The substance for nucleic acid detection is a nucleic acid of a virus. The nucleic acid detection is to find out whether the respiratory tract specimen, blood or feces of the patient have the nucleic acid of the virus invaded from the outside to determine whether the patient is infected by the new coronavirus. Thus, once a nucleic acid is detected as "positive", the presence of the virus in the patient is demonstrated.
All organisms contain nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), and the novel coronavirus is a virus that contains only RNA, and the specific RNA sequence in the virus is a marker that distinguishes the virus from other pathogens. If the specific nucleic acid sequence of the novel coronavirus can be detected in the sample from the patient during the detection process, it is suggested that the patient may be infected with the novel coronavirus.
The most common method for detecting the specific sequence of the novel coronavirus is fluorescence quantitative PCR (polymerase chain reaction), and the detection is completed by RNA amplification formed by the PCR technology, so that the sensitivity is higher. The general detection process comprises the steps of collecting, storing and transporting a sample, extracting and detecting nucleic acid of the sample and the like.
The technical problems of false positive and false negative easily occur in nucleic acid detection, wherein the false positive refers to the condition that a patient is not infected with new coronavirus but a positive result occurs in nucleic acid detection, and is caused by cross contamination among samples or nucleic acid contamination in a laboratory in the detection process. False negative means that new coronary pneumonia is supported by clinical symptoms, lung imaging results and even epidemiological history of patients, but the detection result of virus nucleic acid of the patients is negative, and the detection result is inconsistent with clinical results. Laboratory contamination is the most afraid "enemy" of all new people. The continuous operation is not only performed by personnel, but also performed in a laboratory for 24 hours, and particularly after the personnel and the laboratory perform high-intensity operation, the probability of misoperation of the personnel is increased, and the pollution risk of the laboratory is gradually increased. Laboratory personnel talk about aerosol color change, and the pollution is easy and difficult to remove.
Most of the detection steps and structures in the existing new crown nucleic acid detection process are separated, for example, after a sample is collected and simply stored, the sample is uniformly and intensively submitted for detection, and a detection mechanism extracts and detects a large amount of samples. The inspection process is easy to be polluted and the detection time is long. At least 15-20 steps are needed from sample information entry, reagent preparation, sample pretreatment, extraction, plate rotation, cover closing, centrifugation to machine installation, and a large amount of manpower is consumed. The 24-hour continuous premise needs 3 shifts at least, personnel rotate without stopping, however, 8 hours of each shift are exhausted for 8 hours, even more than 8 hours, personnel are exhausted to put on duty, however, 4 times of sample adding and liquid transferring are needed for each tube of reagent configuration, sample liquid transferring and nucleic acid extraction product liquid transferring, 3000 tubes of accurate sample adding and liquid transferring are needed for 1.2 ten thousand times, and the high quality can not be guaranteed effectively. This leads to high requirements of physical strength, professional ability and pressure resistance of people for new crown detection, and people are exhausted.
In the detection process, the detection extraction and detection are manual or semi-manual operation, the sample amount is large, the workload is large, the process is complicated, the detection intensity is heavy, and the like. At present, the problem that the sample cannot be traced after detection also exists, and the schedule is provided for normalizing the nucleic acid detection point. And all manpower is put into a new crown laboratory, all projects give way to the new crown, and the timeliness of detection of other projects cannot be guaranteed.
The laboratory automation degree is low, and although a large amount of nucleic acid extractors and amplifiers are purchased in the laboratory, the laboratory automation level is not improved. However, the automation products in the market are endless, and what the laboratory really needs and what the automation instrument can solve the fundamental problem, and what is selected are difficult problems.
The specification of the invention CN202011380258.0 in China describes a full-automatic nucleic acid detection system, the device comprises a sample pretreatment unit, a sample detection unit, a sample analysis unit and a sample analysis unit, wherein the sample pretreatment unit is used for transferring a sample to be detected to a nucleic acid extraction pore plate; at least one nucleic acid extraction unit for extracting nucleic acid in a sample to be detected on the nucleic acid extraction pore plate; at least one system formulation unit for mixing nucleic acid amplification reagents with the nucleic acids from the nucleic acid extraction unit; at least one nucleic acid detection unit for performing nucleic acid amplification and detection on the nucleic acid from the system preparation unit; a consumable preparation unit for providing consumables to the sample pretreatment unit, the nucleic acid extraction unit, and the system configuration unit; the sample post-processing unit is used for receiving the medical wastes generated by the nucleic acid extracting unit and the nucleic acid detecting unit; and the plurality of transfer units are used for isolating or communicating the transfer units with other units and materials. Because each link of nucleic acid detection is covered, harm to personnel is avoided, and the efficiency is improved.
However, the detection system still has the technical problems of large workload, unclear specific implementation structure and the like.
Disclosure of Invention
In order to solve the technical problems, the invention provides a full-automatic nucleic acid sample processing system, which is intelligent and automatic in integration, integrates steps of nucleic acid extraction, purification, amplification detection, storage and the like into a whole and is automatically carried out, the detection is fast, efficient, safe and accurate, cross contamination or nucleic acid contamination is avoided, a large amount of manpower is saved, and the detection cost is low.
The invention solves the technical problem, and provides a full-automatic nucleic acid sample processing device system, which comprises an outer frame and a platform, wherein the platform is arranged in the frame, and is characterized in that: the upper computer is arranged outside the rack; a reagent temporary storage and configuration mechanism, a reagent transmission buffer mechanism, a sample cup separation processing mechanism, a nucleic acid extraction mechanism and a packaging mechanism are sequentially arranged in the outer rack from left to right, the reagent temporary storage and configuration mechanism is connected with the reagent transmission buffer mechanism, the reagent transmission buffer mechanism is connected with the cup separation processing mechanism, the cup separation processing mechanism is connected with the nucleic acid extraction mechanism, the nucleic acid extraction mechanism is connected with the packaging mechanism, all the mechanisms are arranged on a platform, all the structures are wirelessly connected through line connection and/or network communication, and an instruction is issued through the control of an upper computer;
the reagent temporary storage and configuration mechanism is used for storing, uniformly mixing, subpackaging and establishing a PCR system;
the reagent transfer buffer mechanism is used for transferring the reagent and avoiding pollution. The reagent zone cannot be directly connected to the sample and a buffer zone is required to avoid contamination.
The sample cup separating treatment mechanism is used for sample extraction pretreatment, and is used for carrying out code scanning, cover opening, liquid transferring and cover closing on an original sampling tube;
the nucleic acid extraction mechanism is used for extracting sample nucleic acid;
the packaging mechanism is used for sealing and storing the sample.
The nucleic acid detection laboratory is a P2+ laboratory, a step negative pressure environment, pressure monitoring and aerosol removal. Ultraviolet rays, high-efficiency filtration and the like.
The pressure in the system is negative.
In the optimization scheme, the pressure value is gradient negative pressure, and the pressure values of the temporary reagent storage and configuration mechanism, the sample cup separating and processing mechanism and the nucleic acid extraction mechanism are 0, -10 and-20 PA in sequence.
The whole cabinet of the system is a negative pressure transfer machine, has definite subareas, mechanical design, unidirectional transfer of a transfer window and P2+ micro laboratory formed by gradient negative pressure. The reagent preparation area (reagent temporary storage and configuration mechanism) is an absolute clean area, has no nucleic acid concentration and is positive pressure; the sample preparation area (sample cup processing mechanism) is a contaminated area, and the low nucleic acid concentration area is-10 PA, so that air in the sample preparation area is prevented from flowing to the reagent preparation area. All operations are carried out in a negative pressure loading cabinet, namely the whole process is carried out in a safe P2+ laboratory, the biological safety regulation is met, and the safety and the pollution are avoided.
The reagent temporary storage and configuration mechanism is provided with a material tray, a refrigeration storage container and a manipulator, the material tray and the manipulator are arranged on the platform in parallel, the manipulator is provided with a mechanical arm, a clamping jaw and a pneumatic pump, the mechanical arm is vertically arranged on the platform, the clamping jaw is arranged at the tail end of the mechanical arm, and the pneumatic pump is connected with the rear end of the mechanical arm and drives the mechanical arm to move according to a set mode; the refrigerated storage container is arranged on the platform below the mechanical arm. The structures are connected with each other through lines and wirelessly connected with network communication.
The material tray is used for storing the reagent placed at normal temperature, and 1920 parts of reagent or more can be stored at one time; the reagent refrigeration storage container is used for storing the reagent at a constant temperature of 4 ℃ at a low temperature; the clamping jaw and the pneumatic pump are used for premixing a reagent switch cover and subpackaging the reagent to a 96-pore plate, and the mechanical arm is used for pre-loading the reagent and completing reagent transfer after subpackaging.
The metal bath for cold storage and preservation is a metal bath device (constant-temperature metal bath), and the mechanical arm is a four-axis mechanical arm and can move transversely and vertically.
The structures of the material tray, the mechanical arm and the like are arranged on 1 platform, and corresponding line devices and electrical cabinets are arranged below the platform. The structures are mutually connected in a line mode and a network communication wireless mode, and finally an instruction is given under the control of an upper computer.
The reagent transmission buffer mechanism comprises a sliding rail and a mechanical arm, the sliding rail and the mechanical arm are arranged on the platform side by side, the tail end of the mechanical arm is provided with a clamping jaw for transferring reagents to be temporarily stored and the reagent from the configuration mechanism through the sliding rail, the sliding rail is tiled on the platform, and the mechanical arm is vertically arranged on the platform.
The sample cup separating treatment mechanism is provided with a sample loading disc, tips head loading devices, a liquid transferring device and a cover opening and closing device, and all the structures are distributed on the platform in parallel and are controlled by an upper computer.
the tips head loading device is provided with a tips head frame conveying and feeding device, a jacking device and a tips head frame conveying and discharging device, the tips head frame conveying and feeding device is connected through an upper computer in a control mode, the tips head frame device is jacked to a fixed position through the tips head frame conveying and feeding device, the sample loading disc is placed, the cover opening and closing device is used for placing a sample into the holding clamping jaw, and the liquid transferring device is used for transferring liquid after loading tips.
The sample loading bay can be loaded with 192 samples.
The cover opening and closing device is provided with a four-axis mechanical arm, the tail end of the mechanical arm is provided with 3 clamping jaws, and 1 clamping jaw is used for opening and closing a cover; another 2 clamping jaws for place test tube and cooperation switch lid action temporarily, 3 clamping jaws are located the arm end in parallel.
The 1 clamping jaw of arm is responsible for snatching the sampling tube, thereby the sampling tube is put on 2 clamping jaws in addition and is pressed from both sides the sampling tube bottom tight, and the arm is rotatory uncapped.
The reagent array to be subpackaged is placed on an operation platform below the mechanical arm and is divided into an operation platform array and a low-temperature treatment array. The reagent to be subpackaged is transmitted to the sample cup separating processing mechanism through the reagent transmission buffer mechanism, the operation platform array is an array for current subpackage processing of the cup separating processing mechanism, and the low-temperature processing array is a waiting processing array area when the reagent transmission buffer mechanism is transmitted to the sample cup separating processing mechanism.
The sample cup separating treatment mechanism is provided with negative pressure and HEPA, all the sucked and exhausted gas are efficiently filtered, the pollution is greatly reduced, the adverse effect on the experimental result is caused, and the good protection can be formed for the laboratory staff. Zero contact of detection personnel and samples is realized, automatic upgrading and transformation of a comprehensive power-assisted laboratory are realized, and the samples are transferred from the sampling pipes to the 96-hole plate and are automatically connected with a hospital information system.
The nucleic acid extraction mechanism is provided with a nucleic acid extraction instrument and a reagent placing and moving device which are arranged on the platform in parallel and are connected with each other through an upper computer; reagent is placed and mobile device is equipped with arm and slide rail, and the arm end is equipped with the clamping jaw for reagent that will transfer from sample branch cup processing mechanism through the slide rail is put in the nucleic acid extraction appearance and is carried out nucleic acid and draw, and the arm is established perpendicularly on the platform, and the slide rail tiling is established on the platform, is used for transferring the reagent that divides cup processing mechanism to come from the sample.
The nucleic acid extraction appearance is used for nucleic acid extraction, and reagent is placed and mobile device places and removes reagent, and the arm is conveniently carried out reagent and is placed and extract reagent back reagent and abandon.
The packaging mechanism is provided with a tips head loading device, a liquid transfer device, a film sealing instrument and a centrifugal machine, and all the structures are arranged on the platform in parallel and are mutually connected through an upper computer; placing nucleic acid and the established PCR system at corresponding positions through a mechanical arm, transferring the nucleic acid into a PCR system after a pipette loads tip, then placing the PCR system finished by transferring into a membrane sealing instrument through the mechanical arm, placing into a centrifuge for centrifugation after membrane sealing is finished, and then placing into a temporary storage position of the PCR plate; the nucleic acid after the plate rotation is put into a membrane sealing instrument through a mechanical arm for membrane sealing, and a temporary storage position of the nucleic acid plate is placed.
The tips head loading device is provided with a tips head frame conveying device, a jacking device and a tips head frame conveying device, and is also controlled by an upper computer; when the tips are conveyed, the tips headstock conveying device conveys the tips head loading device to the jacking device through the conveying belt, and the jacking device ascends to a specified height; when the head is conveyed out, the jacking device descends to the starting point of the tips headstock conveying device, and the head is conveyed out through the conveying belt after the electro-optical sensing.
The liquid transfer device is provided with a mechanical arm and a pneumatic pump, the rear end of the mechanical arm is connected with the pneumatic pump, the front end of the mechanical arm is provided with a clamping jaw, and the mechanical arm is vertically arranged on the platform. tips head loading device is used for sampling from sample test tube and transferring to deep hole plate; the mechanical arm is used for pre-loading the reagent and transferring the reagent after subpackaging, the membrane sealing instrument is used for finally packaging the constructed template, and the centrifugal machine is used for centrifuging the reagent.
The packaging mechanism is also provided with a template preparation device, the template preparation device and other structures are arranged on the platform in parallel, and the template preparation is that the extracted nucleic acid is transferred into a well-established PCR system.
All the devices in the mechanism are in wireless connection through line connection and network communication, and finally the command is sent under the control of an upper computer.
The system can also be provided with a waste rack which is positioned in the nucleic acid extraction mechanism and used for containing waste reagents.
The system is provided with a display screen and an AI vision device, controls an electrical part, and the AI vision device is arranged in the sample cup separating processing system and used for scanning a code function, automatically identifying a bar code on the sampling pipe and uploading the bar code to a hospital information system, and automatically starting the AI vision identification function if the bar code fails to be identified by a common code scanning gun. The display screen is arranged on two sides outside the rack and displays the function of scanning code information.
Be equipped with the ultraviolet disinfection lamp in every mechanism in the system, the position is random, and disinfection and sterilization effect, frame outside both sides are equipped with the display screen, play and watch the AI vision ware and sweep a yard effect.
Every mechanism is equipped with HEPA high efficiency filtration system in the system, for the core composition of biological safety cabinet, and its effect is the dust impurity in catching the air and disinfects the purification to it, and the reduction air is clean, reduces the biological pollution risk.
The system controls and transfers related instructions through the steps of reagent refrigeration preservation, transportation, sample cup separation, nucleic acid extraction, template preparation, membrane sealing, centrifugation and the like, and a host and software so as to transfer and complete related operations. The method is used for operations of reagent subpackaging before detection, reagent extraction and backward transfer, sample cup separation treatment, nucleic acid extraction, system construction, membrane sealing centrifugation and the like.
The full-automatic sample processing system is developed aiming at integrating reagent subpackaging, sample cup separating processing and nucleic acid extraction, is provided with the independent control host, completes reagent subpackaging, reagent extraction backward transfer, sample cup separating processing, nucleic acid extraction, system construction, membrane sealing centrifugal operation and the like through intelligent software, and can efficiently, safely and accurately realize sample processing without manual intervention. The integrated design of a plurality of mechanisms, compact structure is pleasing to the eye, has the characteristics of integrated desktop, does not need complicated PCR laboratory, and nucleic acid extraction and amplification analysis detect the full flow can directly be gone on in the device system.
The template treated by the method can be used for RT-PCR (reverse transcription-polymerase chain reaction) machine.
The system of the invention treats the sample with a treatment flux of more than 4000 tubes/day, or with a treatment flux of more than 8000 tubes/day. Automatic detection, the handling capacity is big, and the zero contact of measurement personnel and sample has practiced thrift a large amount of manpowers when avoiding polluting, detects with low costsly.
Drawings
FIG. 1 is a block diagram of the system detection process of the present invention
FIG. 2 is a schematic diagram of the front structure of the system of the present invention
FIG. 3 is a top view of the system of the present invention
FIG. 4 is a schematic view of the structure of the nucleic acid extraction mechanism of the present invention
FIG. 5 is a schematic view of the sample dispensing mechanism of the present invention
FIG. 6 is a schematic view of the structure of a reagent buffer and allocation mechanism according to the present invention
FIG. 7 is a schematic view of the structure of a buffer mechanism for transferring a reagent according to the present invention
FIG. 8 is a top view of the sample dispensing mechanism of the present invention
FIG. 9 is a top view of a packaging mechanism according to the present invention
The labels in the figure are specifically:
1. an outer frame, 2 reagent temporary storage and configuration mechanisms, 3 reagent transmission buffer mechanisms, 4 sample cup separation processing mechanisms, 5 nucleic acid extraction mechanisms, 6 packaging mechanisms, 7 display screens, 8 pulleys, 9 visual windows, 10 cabinet doors, 11 material trays, 12 reagent transmission areas, 13 mechanical arms, 14 pre-packaged reagent temporary storage areas, 15 sample cup separation processing areas, 16 waste material frames, 17 mold sealing centrifugal areas, 18 tips head loading areas, 19 nucleic acid extraction areas, 20 nucleic acid extraction instruments, 21 clamping jaws, 22 deep holes, reagents, 23 trays, 24 film sealing instruments, 25 centrifuge machines, 26 liquid transfer devices, 27 tips head loading devices, 28 switch covers, 29 sample loading tray positions, 30 slide rail slide rails
Detailed Description
The present invention will be further described with reference to the following embodiments:
example 1
A full-automatic nucleic acid sample processing device system is provided with an outer frame, wherein a reagent loading and configuration temporary storage mechanism, a reagent transfer buffer mechanism, a sample cup separating processing mechanism, a nucleic acid extracting mechanism and a packaging mechanism are sequentially arranged in the outer frame, the reagent loading and configuration temporary storage mechanism is connected with the reagent transfer buffer mechanism, the reagent transfer buffer mechanism is connected with the cup separating processing mechanism, the cup separating processing mechanism is connected with the nucleic acid extracting mechanism, and the nucleic acid extracting mechanism is connected with the packaging mechanism; the platform is arranged in the rack, the upper computer is arranged outside the rack, all the mechanisms are arranged on the platform, all the structures are mutually connected through a line and/or a network communication wireless connection, and an instruction is issued through the upper computer;
the temporary reagent storage and configuration mechanism is used for storing, uniformly mixing, subpackaging and establishing a PCR system; the reagent transfer buffer mechanism is used for avoiding pollution, the reagent area can not be directly connected with the sample, and a buffer area is needed to avoid pollution. The sample cup separating treatment mechanism is used for sample extraction pretreatment, and is used for carrying out code scanning, cover opening, liquid transferring and cover closing on an original sampling tube; a nucleic acid extraction mechanism for nucleic acid extraction; and the packaging mechanism is used for sealing and storing the sample.
Nucleic acid "extraction" is also one of the key elements for successful detection, and is closely related to the collection and storage of virus samples. Before nucleic acid extraction and amplification, virus inactivation is required to be carried out on a sample, wherein the virus inactivation comprises incubation at the temperature of 60-65 ℃ for 20-30 min, or virus inactivation and storage are carried out by using a sampling solution carried by the kit.
The pressure in the system is negative pressure, the pressure value is gradient negative pressure, and the pressure values of the temporary reagent storage and configuration mechanism, the sample cup separating and processing mechanism and the nucleic acid extraction mechanism are 0, -10 and-20 PA in sequence.
The whole cabinet of the system is a negative pressure transfer machine, has definite subareas, mechanical design, unidirectional transfer of a transfer window and P2+ micro laboratory formed by gradient negative pressure. The reagent preparation area (reagent temporary storage and configuration mechanism) is an absolute clean area, has no nucleic acid concentration and is positive pressure; the sample preparation area (sample cup processing mechanism) is a contaminated area, and the low nucleic acid concentration area is-10 PA, so that air in the sample preparation area is prevented from flowing to the reagent preparation area. All operations are carried out in a negative pressure loading cabinet, namely the whole process is carried out in a safe P2+ laboratory, the biological safety regulation is met, and the safety and the pollution are avoided.
The packaging mechanism is used for template preparation and centrifugal temporary storage.
Sample divides cup processing mechanism from taking negative pressure and HEPA, and all inhale and exhaust gas all passes through high efficiency filter, and greatly reduced pollutes and causes adverse effect to the experimental result, also can form good protection to laboratory staff. Zero contact of detection personnel and samples is realized, automatic upgrading and transformation of a comprehensive power-assisted laboratory are realized, and the samples are transferred from the sampling pipe to the 96-hole plate and are connected with a hospital information system. The nucleic acid detection laboratory is in a negative pressure environment, monitors pressure, keeps air flowing and eliminates aerosol. Ultraviolet ray, filtering, air sterilizing machine, etc.
The temporary reagent loading and configuration storage mechanism comprises 1 reagent tray which is mainly used for storing the reagent placed at normal temperature; 1 reagent refrigeration storage part for storing the reagent at4 ℃ constant temperature at low temperature; 1 mechanical arm, a clamping jaw and a pneumatic pump are arranged on the mechanical arm, is used for opening and closing the pre-mixing reagent and subpackaging the pre-mixing reagent into a 96-pore plate; the material tray and the mechanical arm are arranged on the platform in parallel, the mechanical arm is provided with a mechanical arm, a clamping jaw and a pneumatic pump, the mechanical arm is vertically arranged on the platform, the clamping jaw is arranged at the tail end of the mechanical arm, and the pneumatic pump is connected with the rear end of the mechanical arm and drives the mechanical arm to move according to a set mode; the refrigerated storage container is arranged on the platform below the mechanical arm. The structures are connected with each other through lines and wirelessly connected with network communication. Wherein 2 four-axis arms for the transfer of reagent is accomplished in preloading reagent and partial shipment.
The reagent transmission buffer mechanism comprises a sliding rail and a mechanical arm, the sliding rail and the mechanical arm are arranged on the platform side by side, the tail end of the mechanical arm is provided with a clamping jaw for transferring reagents to be temporarily stored and the reagent from the configuration mechanism through the sliding rail, the sliding rail is tiled on the platform, and the mechanical arm is vertically arranged on the platform.
The sample cup separating processing mechanism comprises two sample loading disc positions which can load 192 samples in total; 1 tips head loading system, comprising tips head frame transfer device, lifting device, tips head frame transfer device; a pipetting system: the device comprises 1 four-shaft mechanical arm and a pneumatic pump, wherein the pneumatic pump is used for sampling from a sample test tube and transferring the sample to a deep hole plate; the cover opening and closing system comprises: the test tube testing machine comprises 1 four-axis mechanical arm, a clamping jaw is arranged at the tail end of the mechanical arm and used for opening and closing a cover, and 2 clamping jaws are used for temporarily placing a test tube and matching with the opening and closing of the cover; a spent TIP storage box;
the device is specifically provided with a sample loading disc, a tips head loading device, a liquid transfer device and a cover opening and closing device, wherein all the structures are distributed on a platform in parallel and are controlled by an upper computer.
the tips head loading device is provided with a tips head frame conveying and feeding device, a jacking device and a tips head frame conveying and discharging device, the tips head frame conveying and feeding device is connected through an upper computer in a control mode, the tips head frame device is jacked to a fixed position through the tips head frame conveying and feeding device, the sample loading disc is placed, the cover opening and closing device is used for placing a sample into the holding clamping jaw, and the liquid transferring device is used for transferring liquid after loading tips. The sample loading bay can be loaded with 192 samples.
The cover opening and closing device is provided with a four-axis mechanical arm, the tail end of the mechanical arm is provided with 3 clamping jaws, and 1 clamping jaw is used for opening and closing a cover; another 2 clamping jaws for place test tube and cooperation switch lid action temporarily, 3 clamping jaws are located the arm end in parallel.
The 1 clamping jaw of arm is responsible for snatching the sampling tube, thereby the sampling tube is put on another 2 clamping jaws and is pressed from both sides the sampling tube bottom tightly, and the arm is rotatory uncaps.
The reagent array to be subpackaged is placed on an operation platform below the mechanical arm and is divided into an operation platform array and a low-temperature treatment array. The reagent to be subpackaged is transmitted to the sample cup separating processing mechanism through the reagent transmission buffer mechanism, the operation platform array is an array for current subpackage processing of the cup separating processing mechanism, and the low-temperature processing array is a waiting processing array area when the reagent transmission buffer mechanism is transmitted to the sample cup separating processing mechanism.
The nucleic acid extraction mechanism comprises 1 nucleic acid extraction instrument of Miyaoji EXM6000, which is mainly used for nucleic acid extraction; the reagent placing and moving system comprises 1 four-shaft mechanical arm capable of transversely moving, and a clamping jaw is arranged on 1 mechanical arm, so that the reagent placing and extracting are convenient and the reagent is discarded; the specific nucleic acid extraction mechanism is provided with a nucleic acid extraction instrument and a reagent placing and moving device which are arranged on a platform in parallel and are mutually connected through an upper computer; reagent is placed and mobile device is equipped with arm and slide rail, and the arm end is equipped with the clamping jaw for reagent that will transfer from sample branch cup processing mechanism through the slide rail is put in the nucleic acid extraction appearance and is carried out nucleic acid and draw, and the arm is established perpendicularly on the platform, and the slide rail tiling is established on the platform, is used for transferring the reagent that divides cup processing mechanism to come from the sample. The nucleic acid extraction appearance is used for nucleic acid extraction, and reagent is placed and mobile device places and removes reagent, and the arm is conveniently carried out reagent and is placed and extract reagent back reagent and abandon.
The packaging mechanism comprises a 1 tips head loading system, a tip head frame conveying and feeding device, a jacking device and a tips head frame conveying and discharging device; the liquid transfer system comprises 1 four-shaft mechanical arm and a pneumatic pump, wherein the pneumatic pump is used for sampling from a sample test tube and transferring the sample to a deep hole plate; 1 four-axis arm, the transfer of reagent is accomplished in mainly used pre-load reagent and partial shipment: 1 film sealing instrument is mainly used for final encapsulation of the constructed template; 1 DD-4 centrifuge of Sichuan family.
Specifically, the packaging mechanism is provided with a tips head loading device, a liquid transfer device, a film sealing instrument and a centrifuge, and all the structures are arranged on the platform in parallel and are mutually connected through an upper computer; placing nucleic acid and the established PCR system at corresponding positions through a mechanical arm, transferring the nucleic acid into a PCR system after a tip is loaded by a liquid transferring device, then placing the PCR system finished by transferring the plate into a membrane sealing instrument through the mechanical arm, placing the PCR system finished by membrane sealing into a centrifugal machine for centrifugation after the membrane sealing is finished, and then placing a temporary storage position of the PCR plate; the nucleic acid after the plate rotation is put into a membrane sealing instrument through a mechanical arm for membrane sealing, and a temporary storage position of the nucleic acid plate is placed.
the tips head loading device is provided with a tips head frame conveying device, a jacking device and a tips head frame conveying device, and is also controlled by an upper computer; when the tips are conveyed, the tips head frame conveying device conveys the tips head loading device to the jacking device through the conveying belt, and the jacking device ascends to a specified height; when the head is conveyed out, the jacking device descends to the starting point of the tips headstock conveying device, and the head is conveyed out through the conveying belt after the electro-optical sensing.
And the tips head loading device is provided with a tips head frame conveying and feeding device, a jacking device and a tips head frame conveying and discharging device which are also controlled by the upper computer. When the tips are conveyed in, the tips headstock conveying device conveys the tips head loading device to the jacking device through the conveying belt, and the jacking device ascends to the specified height. When the information is transmitted out, the jacking device descends to the starting point of the tips headstock conveying device, and the information is transmitted out through the conveying belt after the information is sensed by the electro-optic sensor.
The liquid transfer device is provided with a mechanical arm and a pneumatic pump, the rear end of the mechanical arm is connected with the pneumatic pump, the front end of the mechanical arm is provided with a clamping jaw, and the mechanical arm is vertically arranged on the platform. tips head loading device is used for sampling from sample test tube and transferring to deep hole plate; the mechanical arm is used for pre-loading the reagent and transferring the reagent after subpackaging, the membrane sealing instrument is used for finally packaging the constructed template, and the centrifugal machine is used for centrifuging the reagent.
The packaging mechanism is also provided with a template preparation device, the template preparation device and other structures are arranged on the platform in parallel, and the template preparation is that the extracted nucleic acid is transferred into a PCR system which is established before.
All the devices in the mechanism are in wireless connection through line connection and network communication, and finally the command is sent under the control of an upper computer.
Example 2
Other contents are as in embodiment 1, a full-automatic nucleic acid sample processing device system, which comprises an outer frame, wherein a reagent loading and configuration temporary storage mechanism, a reagent transfer buffer mechanism, a sample cup separating processing mechanism, a nucleic acid extracting mechanism and a packaging mechanism are sequentially arranged in the outer frame, the reagent loading and configuration temporary storage mechanism is connected with the reagent transfer buffer mechanism, the reagent transfer buffer mechanism is connected with the cup separating processing mechanism, the cup separating processing mechanism is connected with the nucleic acid extracting mechanism, and the nucleic acid extracting mechanism is connected with the packaging mechanism;
the temporary reagent storage and configuration mechanism is used for storing, uniformly mixing, subpackaging and establishing a PCR system; a reagent transfer buffer mechanism for avoiding contamination, the reagent zone not being directly connectable to the sample, a buffer zone being required to avoid contamination. The sample cup separating treatment mechanism is used for sample extraction pretreatment, and is used for carrying out code scanning, cover opening, liquid transferring and cover closing on an original sampling tube; a nucleic acid extraction mechanism for nucleic acid extraction; and the packaging mechanism is used for sealing and storing the sample.
Nucleic acid "extraction" is also one of the key links for successful detection, and is closely related to the collection and preservation of virus samples. Before nucleic acid extraction and amplification, virus inactivation is required to be carried out on a sample, wherein the virus inactivation comprises incubation at the temperature of 60-65 ℃ for 20-30 min, or virus inactivation and storage are carried out by using a sampling solution carried by the kit.
The packaging mechanism is used for template preparation and centrifugal temporary storage.
The sample cup separating treatment mechanism is provided with negative pressure and HEPA, all the sucked and exhausted gas are efficiently filtered, the pollution is greatly reduced, the adverse effect on the experimental result is caused, and the good protection can be formed for the laboratory staff. Zero contact of detection personnel and samples is realized, automatic upgrading and transformation of a comprehensive power-assisted laboratory are realized, and the samples are transferred from the sampling pipe to the 96-hole plate and are connected with a hospital information system. The nucleic acid detection laboratory is in a negative pressure environment, monitors pressure, keeps air flowing and eliminates aerosol. Ultraviolet ray, filtering, air sterilizing machine, etc.
The temporary reagent loading and configuration storage mechanism comprises 1 reagent tray which is mainly used for storing the reagent placed at normal temperature; 1 reagent refrigeration storage part is used for storing the reagent at a constant temperature of 4 ℃ at a low temperature; 1 mechanical module, wherein the module is provided with a clamping jaw and a pneumatic pump and is used for opening and closing a cover of a premixed reagent and subpackaging a 96-pore plate; 2 four-axis arms for the transfer of reagent is accomplished in pre-load reagent and partial shipment.
The sample cup separating processing mechanism comprises two sample loading disc positions which can load 192 samples in total; 1 tips head loading system, comprising tips head frame transfer device, lifting device, tips head frame transfer device; a pipetting system: the device comprises 1 four-axis mechanical arm and a pneumatic pump, wherein the pneumatic pump is used for sampling from a sample test tube and transferring the sample test tube into a deep hole plate; the cover opening and closing system comprises: the test tube testing machine comprises 1 four-axis mechanical arm, a clamping jaw is arranged at the tail end of the mechanical arm and used for opening and closing a cover, and 2 clamping jaws are used for temporarily placing a test tube and matching with the opening and closing of the cover; useless TIP head receiver.
The packaging mechanism is provided with a tips head loading device, a liquid transfer device, a film sealing instrument and a centrifugal machine, and is controlled by an upper computer. The method is characterized in that the connection is controlled through upper computer software, nucleic acid and the established PCR system are placed at corresponding positions through a mechanical arm, the nucleic acid is transferred to the PCR system after a tip is loaded by a liquid transferring device, then the PCR system finished by transferring is placed into a membrane sealing instrument through the mechanical arm, the membrane sealing instrument is placed into a centrifugal machine for centrifugation after membrane sealing is finished, and then the temporary storage position of the PCR plate is placed. The nucleic acid after plate transfer is put into a membrane sealing instrument through a mechanical arm for membrane sealing, and a nucleic acid plate temporary storage position is placed.
the tips head loading device is provided with a tips head frame conveying and feeding device, a jacking device and a tips head frame conveying and discharging device, and is also controlled by an upper computer. When the tips are conveyed, the tips head frame conveying device conveys the tips head loading device to the jacking device through the conveying belt, and the jacking device ascends to the specified height. When the information is transmitted out, the jacking device descends to the starting point of the tips headstock conveying device, and the information is transmitted out through the conveying belt after the information is sensed by the electro-optic sensor.
The nucleic acid extraction mechanism comprises 1 nucleic acid extraction instrument of Miyaoji EXM6000, which is mainly used for nucleic acid extraction; the reagent placing and moving system comprises 1 four-shaft mechanical arm capable of transversely moving, and a clamping jaw is arranged on 1 mechanical arm, so that the reagent placing and extracting are convenient and the reagent is discarded; and the waste material rack is positioned in the nucleic acid extraction mechanism and used for storing waste reagents.
The packaging mechanism comprises a 1 tips head loading system, a tip head frame conveying and feeding device, a jacking device and a tips head frame conveying and discharging device; the liquid transfer system comprises 1 four-shaft mechanical arm and a pneumatic pump, wherein the pneumatic pump is used for sampling from a sample test tube and transferring the sample to a deep hole plate; 1 four-axis arm, the transfer of reagent is accomplished in mainly used pre-load reagent and partial shipment: 1 film sealing instrument is mainly used for final packaging of the constructed template; 1 DD-4 centrifuge of Sichuan family.
The system is also provided with a display screen which is positioned on two sides of the front outside the rack and correspondingly displays the code scanning information of the AI visual device, and the AI visual device is arranged in the sample cup separating processing system and used for scanning the code function. The newspaper 8K linear array camera is mainly used for sample tube bar code identification (ProNAT 4000); the system is provided with a display screen and an AI vision device, the AI vision device is arranged in the sample cup separating processing system, and the display screen is arranged at two sides outside the frame; the display screen and the AI visual device are mutually connected through a line.
The invention discloses an electromagnetic door lock which can automatically position a door to be opened and closed and can fully automatically open and close the door. The whole machine on the rack is provided with an observation window, and the sample is loaded to be an upward-opening door, so that the internal working state of the equipment can be observed conveniently. The electric part is integrated in the lower part of the lower rack, and comprises an industrial control all-in-one machine and various electric elements, so that the operation of a user is facilitated, no extra occupied space is required, and the equipment is more neat and attractive. The electrical equipment is conventional equipment. The automatic door and the switch are conventionally made. The mechanical arm, the pneumatic pump, the clamping jaw, the nucleic acid extractor, the tips head loading system, the sliding rail, the packaging film, the centrifuge and other structures are conventional structures, and the connection mode among the structures which are not described or disclosed is a conventional connection mode in the technical field.
The above examples are merely for clarity of explanation and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. This need not be, nor should it be exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. The utility model provides a full-automatic nucleic acid sample processing apparatus system, is including being equipped with outer frame and platform, and the platform is established in the frame, its characterized in that: the upper computer is arranged outside the rack; a reagent temporary storage and configuration mechanism, a reagent transmission buffer mechanism, a sample cup separation processing mechanism, a nucleic acid extraction mechanism and a packaging mechanism are sequentially arranged in the outer rack from left to right, the reagent temporary storage and configuration mechanism is connected with the reagent transmission buffer mechanism, the reagent transmission buffer mechanism is connected with the cup separation processing mechanism, the cup separation processing mechanism is connected with the nucleic acid extraction mechanism, the nucleic acid extraction mechanism is connected with the packaging mechanism, all the mechanisms are arranged on a platform, all the structures are wirelessly connected through line connection and/or network communication, and an instruction is issued through the control of an upper computer;
the reagent temporary storage and configuration mechanism is used for storing, uniformly mixing, subpackaging and establishing a PCR system; the reagent transfer buffer mechanism is used for transferring the reagent and avoiding pollution; the cup separating processing mechanism is used for sample extraction pretreatment, and is used for scanning a code, opening a cover, transferring liquid and closing the cover on an original sampling tube; the nucleic acid extraction mechanism is used for nucleic acid extraction; the packaging mechanism is used for sealing and storing the sample.
2. The fully automated nucleic acid sample processing device system according to claim 1, wherein: the pressure in the system is negative.
3. The fully automated nucleic acid sample processing device system according to claim 2, wherein: the pressure value is gradient negative pressure, and the pressure values of the temporary reagent storage and configuration mechanism, the sample cup separating and processing mechanism and the nucleic acid extraction mechanism are 0, -10 and-20 PA in sequence.
4. The fully automated nucleic acid sample processing device system according to claim 1, wherein: the reagent temporary storage and configuration mechanism is provided with a material tray, a cold storage container and a mechanical arm, the material tray and the mechanical arm are arranged on the platform side by side, the mechanical arm is provided with a mechanical arm, a clamping jaw and a pneumatic pump, the mechanical arm is vertically arranged on the platform, the clamping jaw is arranged at the tail end of the mechanical arm, the pneumatic pump is connected with the rear end of the mechanical arm and drives the mechanical arm to move according to a set mode, and the cold storage container is arranged on the platform below the mechanical arm and is in wireless connection with network communication through line connection.
5. The fully automated nucleic acid sample processing device system according to claim 1, wherein: the sample cup separating treatment mechanism is provided with a sample loading tray, tips head loading devices, a liquid transferring device and a cover opening and closing device, all the structures are distributed on the platform in parallel and are controlled by an upper computer.
6. The fully automated nucleic acid sample processing device system according to claim 1, wherein: the nucleic acid extraction mechanism is provided with a nucleic acid extraction instrument and a reagent placing and moving device which are arranged on the platform in parallel and are connected with each other through an upper computer; reagent is placed and mobile device is equipped with arm and slide rail, and the arm end is equipped with the clamping jaw for put reagent in nucleic acid extraction appearance and carry out nucleic acid and draw, the arm is established perpendicularly on the platform, and the slide rail tiling is established on the platform, is used for shifting the reagent that comes from sample branch cup processing mechanism.
7. The fully automated nucleic acid sample processing device system according to claim 1, wherein: the packaging mechanism is provided with a tips head loading device, a liquid transfer device, a membrane sealing instrument and a centrifuge, all structures are arranged on a platform in parallel and are connected through upper computer control, nucleic acid and an established PCR system are placed at corresponding positions through a mechanical arm, the nucleic acid is transferred to the PCR system after the liquid transfer device loads tips, then the PCR system completed by transferring the nucleic acid is placed into the membrane sealing instrument through the mechanical arm, the membrane is placed into the centrifuge for centrifugation after the membrane sealing is completed, and then a temporary storage position of the PCR plate is placed. The nucleic acid after the plate rotation is put into a membrane sealing instrument through a mechanical arm for membrane sealing, and a temporary storage position of the nucleic acid plate is placed.
8. The fully automated nucleic acid sample processing device system according to claim 5 or 7, wherein: the tips head loading device is provided with a tips head frame conveying and feeding device, a jacking device and a tips head frame conveying and discharging device, and all the structures are mutually controlled and connected through an upper computer; the liquid-transfering device is equipped with arm and pneumatic pump, and the arm rear end is connected with the pneumatic pump, and the arm is established perpendicularly on the platform.
9. The fully automated nucleic acid sample processing device system according to claim 1, wherein: the system is equipped with display screen and AI vision ware, control electric part, and the AI vision ware is established in sample divides cup processing system for sweep a yard function, but the bar code on the automatic identification sampling pipe uploads to hospital information system, and the display screen is established in the frame outside both sides, shows to sweep a yard information effect.
10. The fully automated nucleic acid sample processing device system according to claim 1, wherein: each mechanism in the system comprises an HEPA high-efficiency filtering system which is used for capturing dust impurities in the air, sterilizing and purifying the dust impurities, restoring the air to be clean and reducing the risk of biological pollution.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116676183A (en) * | 2023-07-31 | 2023-09-01 | 四川徕伯益自动化技术有限公司 | Molecular diagnosis system and molecular diagnosis method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116676183A (en) * | 2023-07-31 | 2023-09-01 | 四川徕伯益自动化技术有限公司 | Molecular diagnosis system and molecular diagnosis method |
| CN116676183B (en) * | 2023-07-31 | 2023-11-10 | 四川徕伯益自动化技术有限公司 | Molecular diagnosis system |
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