CN217989348U - PCR amplification operation cabinet with sample transfer function - Google Patents

Abstract

The utility model relates to a PCR amplification operation cabinet with a sample transmission function. The utility model includes a specimen preparation module, a PCR amplification module and a sample transmission module. The specimen preparation module includes a specimen preparation room, a bottom air chamber, a side air chamber and a first top air chamber. The specimen preparation room is provided with a specimen preparation operation window, The first air inlet and the specimen preparation area, the first top air chamber is provided with a first exhaust device and a first disinfection filter device, the first top air chamber is provided with an upper air outlet and a lower air outlet, and the sample transfer module includes a sample transfer Room, exhaust air channel and make-up air channel. Compared with the prior art, the utility model fills the interior of the entire specimen preparation room with clean gas, so that the samples and reagents are not polluted, thereby preventing cross-contamination between samples in the specimen preparation room; at the same time, the staff can prepare the specimen The samples in the room are transferred to the PCR amplification room through the sample transfer room to prevent the samples from being exposed to the outside air.

Description

PCR amplification operation cabinet with sample transfer function

technical field

The utility model belongs to the technical field of biological sample processing and detection, in particular to a PCR amplification operation cabinet with a sample transmission function.

Background technique

Due to the need to ensure safety and pollution-free during PCR nucleic acid detection, high requirements are placed on the operating environment, and most of the existing PCR nucleic acid detection is carried out in PCR laboratories.

Existing PCR laboratories are generally divided into four separate work areas: reagent preparation area, specimen preparation area, amplification area, and product analysis area. In order to avoid cross-contamination, operators must strictly follow a single direction when entering each work area. , high environmental site requirements, cumbersome and complicated operations, which limit the wide application of PCR nucleic acid detection. In order to solve the above problems, the patent application document CN202020725842.4 discloses a biological safety cabinet for PCR experiments. However, the scheme in this patent application document is not convenient for staff to operate, and there is a risk of cross-contamination among multiple samples.

Therefore, there is an urgent need for a technical solution that can facilitate the operation of the staff and avoid cross-contamination between multiple samples.

Utility model content

The purpose of this utility model is: aiming at the deficiencies of the prior art, the utility model provides a PCR amplification operation cabinet with a sample transmission function, so as to solve the problem that the existing PCR nucleic acid detection is inconvenient for staff to operate and between multiple samples. There is the issue of cross-contamination risk.

In order to achieve the above object, the utility model adopts the following technical solutions:

PCR amplification operation cabinet with sample transfer function, including:

A sample preparation module, the sample preparation module includes a sample preparation room, a bottom air chamber arranged below the sample preparation room, a side air chamber arranged on the side and/or rear of the sample preparation room, and a The first top air cavity above the sample preparation room, the sample preparation room is provided with a sample preparation operation window on the front, the sample preparation room is provided with a first air inlet and a sample preparation area on the inner bottom surface, and the first The air inlet is located in front of the specimen preparation area, the width of the first air inlet corresponds to the width of the specimen preparation operation window, and the interior of the specimen preparation room is connected to the bottom air chamber through the first air inlet. The bottom air chamber communicates with the first top air chamber through the side air chamber, and the first top air chamber is internally provided with a first exhaust device and a first disinfection filter device, and the first The top air chamber is provided with an upper air outlet connected to the outside of the operation cabinet, and the first top air chamber is provided with a lower air outlet connected with the interior of the specimen preparation room;

A PCR amplification module, the PCR amplification module is located on one side of the specimen preparation module, and the PCR amplification module includes a PCR amplification room;

A sample transmission module, the sample transmission module is located between the specimen preparation room and the PCR amplification room, the sample transmission module includes a sample transmission room, a ventilation channel and a supplementary air channel, and the sample transmission room is on one side A first closed opening and a first closed door corresponding to the first closed opening are provided, and a second closed opening and a second closed door corresponding to the second closed opening are provided on the other side of the sample transfer room. A closed door, the sample transmission room communicates with the specimen preparation room through the first closed opening, the sample transmission room communicates with the PCR amplification room through the second closed opening, and the sample transmission room is The rear side of the interior is provided with a transmission exhaust port and a transmission supplementary air outlet. The transmission exhaust port communicates with the outside of the operation cabinet through the exhaust channel, and the transmission supplementary air port communicates with the outside of the operation cabinet through the supplementary air channel. In communication, the ventilation channel is provided with a third ventilation device and a fourth disinfection filter device inside, and the supplementary air channel is provided with a fourth ventilation device and a fifth disinfection filter device inside.

As a preferred solution of the PCR amplification operation cabinet with sample transmission function described in the present invention, the side air chamber is provided with a side air inlet near the inner bottom surface of the specimen preparation room, and the side air inlet of the specimen preparation room is The interior communicates with the side air cavity through the side air inlet, and the width of the side air inlet corresponds to the length of the side of the corresponding specimen preparation area.

As an optimal solution of the PCR amplification operation cabinet with sample transmission function described in the present invention, the sample preparation room is also provided with a sample preparation operation window on the front corresponding to the sample preparation operation window, and the first A top air cavity is provided with a window and door accommodation room in front, and the window and door accommodation room is provided with a window and door through slot corresponding to the sample preparation operation window on the bottom surface, and the inner side of the sample preparation operation window is provided with an upwardly extending To the window door track inside the window door accommodation room, the sample preparation operation window door is slidably arranged at the sample preparation operation window along the window door track, and the window door accommodation room is internally provided with a lifting motor and Lifting conveyor belt, the lifting motor is connected to the specimen preparation operation window through the lifting conveyor belt, and the lifting motor can drive the specimen preparation operation window to reciprocate along the window track through the lifting conveyor belt .

As a preferred solution of the PCR amplification operation cabinet with sample transmission function described in the present invention, the first top air cavity is also provided with an inner air duct inside, and the first air exhaust device includes a first air intake and a first air intake. The first exhaust air outlet, the first top air cavity communicates with the first air exhaust device through the first exhaust air inlet, and the inner air duct is provided with a wind communicating with the first exhaust air outlet on the side. The inner air duct communicates with the air outlet of the upper air duct on the upper side, and the inner air duct communicates with the air outlet of the lower air duct on the lower side. The first disinfection filter device is arranged in the inner air duct internal.

As a preferred solution of the PCR amplification operation cabinet with sample transmission function described in the present invention, the first disinfection filter device is provided at the air outlet of the upper air duct and/or at the air outlet of the lower air duct.

As a preferred solution of the PCR amplification operation cabinet with sample transmission function described in the utility model, the PCR amplification module also includes a second top air cavity arranged above the PCR amplification room, and the PCR amplification The front side of the room is provided with a PCR amplification operation window and a PCR amplification operation window door corresponding to the PCR amplification operation window, and the PCR amplification room is provided with a second air inlet on the side, and the outside of the operation cabinet The second air inlet communicates with the interior of the PCR amplification room through the second air inlet, and the second air inlet is provided with a second disinfection filter device inside, and the second top air chamber is provided with a Add a lower air inlet connected internally, and the second top air cavity is provided with a second ventilation device and a third disinfection filter device inside, and the second ventilation device includes a second ventilation inlet and a second ventilation outlet, The second top air cavity is communicated with the second exhaust device through the second exhaust air inlet, and the second exhaust device is communicated with the outside of the operation cabinet through the second exhaust air outlet.

As a preferred solution of the PCR amplification operation cabinet with sample transmission function described in the present invention, the PCR amplification room is provided with a storage board for placing a PCR instrument inside.

As a preferred solution of the PCR amplification operation cabinet with sample transmission function described in the present invention, it also includes a mobile frame, which is used to support the specimen preparation module and the PCR amplification module, the The mobile frame is arranged below the sample preparation module and the PCR amplification module, and the mobile frame is provided with moving rollers below.

As a preferred solution of the PCR amplification operation cabinet with sample transmission function described in the present invention, the transmission air outlet is located at the lower edge of the inner rear side of the sample transmission room, and the transmission supplementary air outlet is located at the sample transmission room. at the upper edge of the interior rear side of the room.

As a preferred solution of the PCR amplification operation cabinet with sample transmission function described in the present invention, the specimen preparation room, the PCR amplification room and the sample transmission room are provided with air pressure sensors inside.

The utility model at least has the following beneficial effects:

1) In the present utility model, under the action of the first exhaust device, the gas inside the specimen preparation room and/or the gas at the specimen preparation operation window sequentially passes through the first air inlet, the bottom air chamber, the side air chamber, and the first top air chamber. cavity and the first exhaust device; part of the gas is discharged to the inside of the specimen preparation room through the air outlet of the lower air duct, and the gas will be sterilized and sterilized by the first disinfection filter device in the first top air chamber, so that the clean gas is discharged from the air outlet of the lower air duct Discharge and fill the entire specimen preparation room, so that the samples and reagents are not polluted, thereby preventing cross-contamination between samples in the specimen preparation room; another part of the gas is discharged to the outside of the operation cabinet through the air outlet of the upper air duct, due to the first air inlet. The air intake volume is greater than the air output volume of the air outlet of the lower air duct, so that negative pressure is formed inside the specimen preparation room, thereby preventing the gas in the specimen preparation room from being directly discharged from the specimen preparation operation window, and preventing the specimen from being transmitted to the staff through the air; moreover, the work When personnel are preparing specimens, the specimen preparation operation window will not be blocked by isolation glass, which is convenient for the staff to operate;

2) The first air inlet of the utility model is located in front of the specimen preparation area, and the width of the first air inlet corresponds to the width of the specimen preparation operation window. When the gas outside the operation cabinet enters from the specimen preparation operation window, the specimen preparation room When inside, since the first air inlet is in a negative pressure state and its width corresponds to the width of the specimen preparation operation window, the air outside the operation cabinet will flow to the first air inlet, thereby preventing the air outside the operation cabinet from contacting and contaminating the specimen Samples and reagents in the preparation area; at the same time, when the internal air in the specimen preparation room is discharged toward the direction of the specimen preparation operation window, the internal air in the specimen preparation room will flow to the first air inlet due to the negative pressure at the first air inlet place, so as to avoid contact between the internal gas of the specimen preparation room and the staff;

3) The utility model enables the staff to transfer the samples from the specimen preparation room to the PCR amplification room through the sample transfer room by setting the sample transfer module, so as to avoid the exposure of the samples to the outside air and reduce the risk of sample contamination;

4) In this utility model, under the action of the third exhaust device, the gas inside the sample transmission room passes through the transmission exhaust port, and is discharged to the outside of the operation cabinet through the ventilation channel, so that the gas in the sample transmission room is extracted in time to prevent specimen preparation. The room and the PCR amplification room are cross-infected through the sample transmission room, and the gas will be sterilized and sterilized through the fourth disinfection filter device in the ventilation channel to ensure that the discharged gas is clean;

5) In this utility model, under the action of the fourth exhaust device, the gas outside the operation cabinet passes through the supplementary air passage, and is discharged to the inside of the sample transmission room through the transmission supplementary air outlet, and the gas will pass through the fifth disinfection filter in the supplementary air passage The device is disinfected and sterilized, so that the sample transfer room is filled with clean gas, reducing the air pressure difference between the sample transfer room, the specimen preparation room and the PCR amplification room, avoiding a large amount of gas entering the sample transfer room, and reducing the risk of cross-contamination.

Description of drawings

Fig. 1 is a schematic structural view of the operation cabinet in the utility model.

Fig. 2 is the front view of the operation cabinet in the utility model.

Fig. 3 is a front view of the operation cabinet in the utility model, and a section of part of the structure is carried out.

FIG. 4 is a schematic diagram of gas flow based on FIG. 3 .

Fig. 5 is a sectional view along A-A direction in Fig. 2 .

FIG. 6 is a schematic diagram of gas flow based on FIG. 5 .

Fig. 7 is a sectional view along B-B direction in Fig. 2 .

FIG. 8 is a schematic diagram of gas flow based on FIG. 7 .

FIG. 9 is an enlarged view of area C in FIG. 3 .

Fig. 10 is a side view of the sample transmission module, and a section view of part of the structure.

FIG. 11 is a schematic diagram of gas flow based on FIG. 10 .

In the picture:

1-specimen preparation module; 11-specimen preparation room; 111-first air inlet; 112-specimen preparation operation window; 113-specimen preparation operation window; 114-specimen preparation area; 12-bottom air cavity; 13-side Air chamber; 131-side air inlet; 14-first top air chamber; 141-first exhaust device; 142-inner air duct; 1421-air inlet of air duct; 1422-air outlet of upper air duct; ; 1424-the first disinfection filter device; 15-window and door storage room; 151-lifting motor;

2-PCR amplification module; 21-PCR amplification room; 211-second air inlet; 2111-second disinfection filter device; 212-PCR amplification operation window; 213-PCR amplification operation window; 214-storage board ; 22-the second top air cavity; 221-the second exhaust device; 2211-the second exhaust outlet; 222-the third disinfection filter device; 223-the lower air inlet;

3-sample transmission module; 31-sample transmission room; 311-first closed port; 312-first closed door; 313-second closed door; 314-second closed door; Tuyere; 32-ventilation channel; 321-the third ventilation device; 322-the fourth disinfection filter device; 33-supplement air channel; 331-the fourth ventilation device; 332-the fifth disinfection filter device;

4-moving frame body; 41-moving roller;

5-power socket;

6-ultraviolet disinfection lamp;

7- Control Panel.

detailed description

In order to make the technical solutions and advantages of the utility model clearer, the utility model and its beneficial effects will be further described in detail below in conjunction with the specific implementation and the accompanying drawings, but the implementation of the utility model is not limited thereto.

As shown in Figures 1 to 11, the PCR amplification operation cabinet with sample transmission function includes:

The sample preparation module 1, the sample preparation module 1 includes a sample preparation room 11, a bottom air chamber 12 arranged below the sample preparation room 11, a side air chamber 13 arranged on the side and/or rear of the sample preparation room 11, and a The first top air cavity 14 above the specimen preparation room 11, the specimen preparation room 11 is provided with a specimen preparation operation window 112 and a control panel 7 on the front, and the specimen preparation room 11 is provided with a first air inlet 111 and a specimen preparation area on the inner bottom surface 114, the first air inlet 111 is located in front of the specimen preparation area 114, the width of the first air inlet 111 corresponds to the width of the specimen preparation operation window 112, and the interior of the specimen preparation room 11 communicates with the bottom air chamber 12 through the first air inlet 111 , the bottom wind chamber 12 communicates with the first top wind chamber 14 through the side wind chamber 13, and the first top wind chamber 14 is provided with a first exhaust device 141 and a first disinfection filter device 1424 inside, and the first top wind chamber 14 is in An upper air duct outlet 1422 communicating with the outside of the operation cabinet is provided on the upper side, and a lower air duct outlet 1423 communicating with the interior of the specimen preparation room 11 is provided below the first top air cavity 14;

PCR amplification module 2, the PCR amplification module 2 is located on one side of the specimen preparation module 1, and the PCR amplification module 2 includes a PCR amplification room 21;

The sample transmission module 3, the sample transmission module 3 is located between the specimen preparation room 11 and the PCR amplification room 21, the sample transmission module 3 includes a sample transmission room 31, a ventilation channel 32 and a supplementary air channel 33, and the sample transmission room 31 is set on one side There is a first closed opening 311 and a first closed door 312 corresponding to the first closed opening 311, and the sample transfer room 31 is provided with a second closed opening 313 and a second closed opening corresponding to the second closed opening 313 on the other side. Door 314, the sample transfer room 31 communicates with the specimen preparation room 11 through the first closed port 311, the sample transfer room 31 communicates with the PCR amplification room 21 through the second closed port 313, and the sample transfer room 31 is provided with a transmission exhaust on the inner rear side Port 315 and transmission supplementary air port 316, transmission exhaust port 315 communicates with the outside of the operation cabinet through the ventilation channel 32, transmission supplementary air port 316 communicates with the outside of the operation cabinet through the supplementary air channel 33, and the exhaust channel 32 is provided with a third exhaust device inside 321 and a fourth sterilizing filter device 322 , the supplementary air channel 33 is provided with a fourth exhaust device 331 and a fifth sterilizing filter device 332 inside.

The working principle of this embodiment is:

1) As shown in Figure 4 and Figure 6, start the first ventilation device 141, under the action of the first ventilation device 141, the gas inside the specimen preparation room 11 and the gas at the specimen preparation operation window 112 pass through the first air inlet in sequence 111, the bottom air chamber 12, the side air chamber 13, the first top air chamber 14, and the first exhaust device 141; a part of the gas is discharged to the inside of the specimen preparation room 11 through the air outlet 1423 of the lower air passage, so that the first air inlet 111 Negative pressure is formed, and at the same time, the gas will be sterilized and sterilized through the first disinfection filter device 1424 in the first top air chamber 14, and then the clean gas will be discharged from the air outlet 1423 of the lower air duct and fill the entire specimen preparation room 11, so that samples and reagents will not Contamination, thereby preventing cross-contamination between samples in the specimen preparation room 11; another part of the gas is discharged to the outside of the operation cabinet through the air outlet 1422 of the upper air passage, so that the air intake of the first air inlet 111 is greater than that of the air outlet 1423 of the lower air passage. The amount of air output makes the interior of the specimen preparation room 11 form a negative pressure; wherein, when the gas outside the operation cabinet enters the specimen preparation room 11 from the specimen preparation operation window 112, since the first air inlet 111 is in a negative pressure state , so the air outside the operation cabinet will flow to the first air inlet 111, thereby preventing the air outside the operation cabinet from contacting and polluting the samples and reagents in the specimen preparation area 114; When exhausting in the opposite direction, since the first air inlet 111 is in a negative pressure state, the internal air in the specimen preparation room 11 will flow to the first air inlet 111, thereby preventing the internal air in the specimen preparation room 11 from contacting the staff;

2) Subsequently, the staff prepares samples in the specimen preparation room 11 for PCR amplification; after the sample preparation is completed, the first closed door 312 is opened and the sample is moved to the inside of the sample transmission room 31 through the first closed port 311, and closed first closing door 312;

3) As shown in Figure 11, start the third exhaust device 321, under the action of the third exhaust device 321, the gas inside the sample transmission room 31 passes through the transmission exhaust port 315, and is discharged to the outside of the operation cabinet through the exhaust channel 32, Thereby, the gas with part of the gas in the sample preparation room 11 inside the sample transfer room 31 is extracted; at the same time, the fourth exhaust device 331 is activated, and under the action of the fourth exhaust device 331, the gas outside the operation cabinet passes through the supplementary air channel 33 and passes through The transmission supplementary air port 316 is discharged to the inside of the sample transmission room 31, and the gas will pass through the fifth disinfection filter device 332 in the supplementary air channel 33 for disinfection and sterilization, so that the sample transmission room 31 is filled with clean gas, thereby reducing the number of samples in the sample transmission room 31. There is an air pressure difference between the specimen preparation room 11 and the PCR amplification room 21;

4) After ventilating the sample transmission room 31 for a period of time, close the third ventilation device 321 and the fourth ventilation device 331, open the second closed door 314 and move the sample to the inside of the PCR amplification room 21 through the second closed port 313 , close the second closing door 314;

5) Subsequently, the staff performs PCR amplification inside the PCR amplification room 21; after the PCR amplification is completed, the sample is sealed, and the sample is moved to the next process.

Preferably, the side air chamber 13 is provided with a side air inlet 131 near the inner bottom surface of the specimen preparation room 11, and the interior of the specimen preparation room 11 communicates with the side air chamber 13 through the side air inlet 131, and the width of the side air inlet 131 is The length corresponds to the side length of the corresponding specimen preparation area 114 . Through the above settings, the gas in the specimen preparation area 114 can enter the side air chamber 13 through the side air inlet 131 in time, thereby further avoiding cross-contamination of the samples and reagents in the specimen preparation area 114 and reducing the amount of air in the specimen preparation area 11. The internal gas of the sample preparation room 11 may be discharged toward the sample preparation operation window 112, thereby preventing the internal gas of the sample preparation room 11 from contacting the staff.

Preferably, the specimen preparation room 11 is also provided with a specimen preparation operation window 113 corresponding to the specimen preparation operation window 112 on the front, and the first top air chamber 14 is provided with a window and door accommodation room 15 in front, and the window and door accommodation room 15 is The bottom surface is provided with a window channel corresponding to the specimen preparation operation window 113, and the inner side of the specimen preparation operation window 112 is provided with a window track extending upward to the inside of the window accommodation room 15, and the specimen preparation operation window 113 is along the window track. Slidingly arranged at the specimen preparation operation window 112, the window door accommodation room 15 is provided with a lifting motor 151 and a lifting conveyor belt inside, the lifting motor 151 is connected to the specimen preparation operation window 113 through the lifting conveyor belt, and the lifting motor 151 can be driven by the lifting conveyor belt The specimen preparation operation window 113 reciprocates along the track of the window. Through the above settings, when the specimen preparation module 1 is in the non-working state, the specimen preparation room 11 can be closed through the specimen preparation operation window door 113, thereby preventing the air outside the operation cabinet from entering the specimen preparation room 11 and causing pollution; at the same time, the specimen preparation operation window The door 113 can reciprocate up and down at the specimen preparation operation window 112 along the window door track.

Preferably, the first top air chamber 14 is also provided with an inner air passage 142 inside, and the first air draft device 141 includes a first air intake and a first air outlet, and the first top air chamber 14 passes through the first air inlet and the first air inlet. The first ventilation device 141 communicates, and the inner air duct 142 is provided with an air duct air inlet 1421 communicating with the first exhaust air outlet on the side, and the inner air duct 142 communicates with the upper air duct air outlet 1422 on the top, and the inner air duct 142 communicates with the upper air duct air outlet 1422 below. The air outlet 1423 of the lower air passage is connected, and the first disinfection filter device 1424 is arranged inside the inner air passage 142 . Through the above configuration, the structure of the inner air channel 142 is conducive to the internal air flow of the first top air chamber 14, improves the disinfection effect of the gas, and ensures that the air discharged from the air outlet 1422 of the upper air channel and the air outlet 1423 of the lower air channel is clean air.

Preferably, a first disinfection filtering device 1424 is provided at the air outlet 1422 of the upper air passage and/or at the air outlet 1423 of the lower air passage. Through the above arrangement, the disinfection effect of the gas is further improved, and it is ensured that the air discharged from the air outlet 1422 of the upper air passage and the air outlet 1423 of the lower air passage is clean air.

Preferably, the PCR amplification module 2 also includes a second top air cavity 22 arranged above the PCR amplification room 21, and the PCR amplification room 21 is provided with a PCR amplification operation window 212 on the front and corresponding to the PCR amplification operation window 212. The PCR amplification operation window door 213 that is set, the PCR amplification room 21 is provided with the second air inlet 211 on the side, the outside of the operation cabinet communicates with the inside of the PCR amplification room 21 through the second air inlet 211, and the second air inlet 211 A second sterilizing filter device 2111 is provided inside, and the second top air chamber 22 is provided with a lower air inlet 223 communicating with the inside of the PCR amplification room 21 below, and the second top air chamber 22 is provided with a second exhaust device 221 inside And the third sterilizing filter device 222, the second exhaust device 221 includes a second exhaust air inlet and a second exhaust air outlet 2211, the second top air chamber 22 communicates with the second exhaust air inlet 221 through the second exhaust air inlet, and the second exhaust air The device 221 communicates with the outside of the operation cabinet through the second air outlet 2211 . The working principle of the PCR amplification module 2 is: as shown in Figure 4 and Figure 8, start the second exhaust device 221, under the effect of the second exhaust device 221, the gas inside the PCR amplification room 21, the PCR amplification operation window The gas at 212 and the gas at the second air inlet 211 pass through the lower air inlet 223, the second top air cavity 22, and the second exhaust device 221 in sequence, and are discharged to the outside of the operation cabinet through the second exhaust air outlet 2211, so that the PCR The inner top surface of the amplification room 21 forms a negative pressure; at the same time, the gas will pass through the third disinfection filter device 222 in the second top air chamber 22 for disinfection and sterilization, and the gas passing through the second air inlet 211 will pass through the second air inlet 211. The second disinfection filter device 2111 is sterilized and sterilized; after the staff puts the sample into the PCR instrument in the PCR amplification room 21, set the amplification time of the PCR instrument, and close the PCR amplification operation window 213; Under the action of the second air exhaust device 221, the air outside the operation cabinet passes through the second air inlet 211, the lower air inlet 223, the second top air cavity 22 and the second air exhaust device 221 in sequence, and is discharged through the second air exhaust outlet 2211 to The outside of the operation cabinet; at the same time, the gas will be sterilized and sterilized through the second sterilizing filter device 2111 and the third sterilizing filter device 222 in sequence. Through the above arrangement, under the action of the second exhaust device 221, the inner top surface of the PCR amplification room 21 forms a negative pressure, thereby preventing the gas in the PCR amplification room 21 from being directly discharged from the PCR amplification operation window 212, and preventing the specimen from passing through. The air is transmitted to the staff; at the same time, the gas will be sterilized and sterilized by the third disinfection filter device 222 in the second top air chamber 22 to ensure that the discharged gas is clean; moreover, the gas passing through the second air inlet 211 will pass through the second air inlet. The second disinfection filter device 2111 in the tuyere 211 is sterilized and sterilized to ensure that the gas entering the PCR amplification room 21 during the PCR amplification is clean gas.

Preferably, the PCR amplification room 21 is provided with a storage board 214 for placing a PCR instrument inside. Through the above settings, multiple PCR instruments can be placed inside the PCR amplification room 21 to improve work efficiency.

Preferably, a mobile frame body 4 is also included, the mobile frame body 4 is used to support the specimen preparation module 1 and the PCR amplification module 2, the mobile frame body 4 is arranged under the specimen preparation module 1 and the PCR amplification module 2, and the mobile frame body 4 is provided with moving roller 41 below. Through the above arrangement, the staff can move the entire operation cabinet through the moving frame body 4 .

Preferably, the transmission exhaust port 315 is located at the lower edge of the inner rear side of the sample transmission room 31 , and the transmission supplementary air outlet 316 is located at the upper edge of the inner rear side of the sample transmission room 31 . Through the above setting, the transmission exhaust port 315 can remove the gas from the sample in time, and the transmission supplementary air port 316 can discharge clean gas from top to bottom, thereby reducing the risk of sample cross-contamination.

Preferably, the specimen preparation room 11 , the PCR amplification room 21 and the sample transmission room 31 are provided with air pressure sensors inside, and the air pressure sensors are electrically connected to the control panel 7 . Through the above settings, the staff can check the real-time air pressure values of the specimen preparation room 11, the PCR amplification room 21 and the sample transmission room 31 through the control panel 7, so that the third ventilation device 321 and the fourth ventilation device can be adjusted according to the real-time air pressure values 331 operating power to reduce the air pressure difference between the sample transmission room 31 and the sample preparation room 11 and the PCR amplification room 21.

Preferably, the specimen preparation room 11 and/or the PCR amplification room 21 is provided with a power socket 5 inside. Through the above setting, it is convenient for the staff to use the electrified equipment in the specimen preparation room 11 and the PCR amplification room 21, which further facilitates the staff to operate.

Preferably, the specimen preparation room 11 and/or the PCR amplification room 21 is provided with an ultraviolet disinfection lamp 6 inside. Through the above settings, when the specimen preparation module 1 or the PCR amplification room 21 is in a non-working state, the corresponding ultraviolet disinfection lamp 6 can be turned on to perform disinfection and sterilization of the internal environment, thereby avoiding sample cross-contamination.

According to the disclosure and teaching of the above specification, those skilled in the art to which the present utility model belongs can also change and modify the above embodiment. Therefore, the utility model is not limited to the above-mentioned specific implementation manners, and any obvious improvement, replacement or modification made by those skilled in the art on the basis of the utility model belongs to the protection scope of the utility model. In addition, although some specific terms are used in this specification, these terms are only for convenience of description and do not constitute any limitation to the present utility model.

Claims (10)

1. PCR amplification operation cabinet with sample transmission function, its characterized in that includes:

the specimen preparation module (1), the specimen preparation module (1) includes a specimen preparation room (11), a bottom air cavity (12) arranged below the specimen preparation room (11), a side air cavity (13) arranged at the side and/or rear of the specimen preparation room (11), and a first top air cavity (14) arranged above the specimen preparation room (11), the specimen preparation room (11) is provided with a specimen preparation operation window (112) at the front, the specimen preparation room (11) is provided with a first air inlet (111) and a specimen preparation area (114) on the inner bottom surface, the first air inlet (111) is positioned in front of the specimen preparation area (114), the width of the first air inlet (111) corresponds to the width of the specimen preparation operating window (112), the inside of the specimen preparation room (11) is communicated with the bottom air cavity (12) through the first air inlet (111), the bottom air cavity (12) is communicated with the first top air cavity (14) through the lateral air cavity (13), the first top air cavity (14) is internally provided with a first air draft device (141) and a first disinfection and filtration device (1424), the first top air cavity (14) is provided with an upper air duct air outlet (1422) communicated with the outside of the operating cabinet, and the first top air cavity (14) is provided with an upper air duct air outlet (1422) communicated with the inside of the specimen preparation room (11) and communicated with the inside of the specimen preparation room (11) below A lower duct outlet (1423);

a PCR amplification module (2), wherein the PCR amplification module (2) is positioned at one side of the specimen preparation module (1), and the PCR amplification module (2) comprises a PCR amplification chamber (21);

a sample transmission module (3), the sample transmission module (3) is located between the sample preparation room (11) and the PCR amplification room (21), the sample transmission module (3) comprises a sample transmission room (31), an air draft channel (32) and an air supplement channel (33), the sample transmission room (31) is provided with a first closed port (311) and a first closed door (312) correspondingly arranged with the first closed port (311) on one side, the sample transmission room (31) is provided with a second closed port (313) and a second closed door (314) correspondingly arranged with the second closed port (313) on the other side, the sample transmission room (31) is communicated with the sample preparation room (11) through the first closed port (311), the sample transmission room (31) is communicated with the PCR amplification room (21) through the second closed port (313), the sample transmission room (31) is provided with a transmission air draft port (315) and a transmission air supplement port (316) on the inner rear side, the transmission air draft port (315) is communicated with the PCR amplification room (21) through a fourth air supplement channel (322), and a disinfection operation device is provided with a fourth air exhaust channel (316) and a disinfection device (322) through the air draft channel (322), the air supplementing channel (33) is internally provided with a fourth air draft device (331) and a fifth disinfection filtering device (332).

2. The PCR amplification operation cabinet with sample transmission function according to claim 1, wherein: the side air cavity (13) is provided with a side air inlet (131) at a position close to the inner bottom surface of the specimen preparation room (11), the inside of the specimen preparation room (11) is communicated with the side air cavity (13) through the side air inlet (131), and the width of the side air inlet (131) corresponds to the length of the side length of the corresponding specimen preparation area (114).

3. The PCR amplification operation cabinet with sample transmission function according to claim 1, wherein: between sample preparation (11) openly still be provided with sample preparation operation window (112) corresponding sample preparation operation window (113) that sets up, first top wind chamber (14) are provided with the window in the front and hold between (15), the window hold between (15) be provided with in the bottom surface with sample preparation operation window (113) corresponding window leads to the groove, the inboard of sample preparation operation window (112) is provided with upwards extends to the inside window track of (15) between the window holds, sample preparation operation window (113) are followed the window track slides and sets up sample preparation operation window (112) department, window hold between (15) and be provided with elevator motor (151) and lifting conveyor belt in inside, elevator motor (151) pass through lifting conveyor belt with sample preparation operation window (113) transmission is connected, elevator motor (151) can pass through lifting conveyor belt drives sample preparation operation window (113) are followed the window track is reciprocating motion.

4. The PCR amplification operation cabinet with sample transmission function according to claim 1, wherein: first top wind chamber (14) still are provided with interior wind channel (142) in inside, first updraft ventilator (141) include first convulsions air intake and first convulsions air outlet, first top wind chamber (14) pass through first convulsions air intake with first updraft ventilator (141) intercommunication, interior wind channel (142) be provided with in the side with wind channel air intake (1421) of first convulsions air outlet intercommunication, interior wind channel (142) on the top with last wind channel air outlet (1422) intercommunication, interior wind channel (142) under with wind channel air outlet (1423) intercommunication down, first disinfection filter equipment (1424) set up inside wind channel (142).

5. The PCR amplification cabinet with sample transmission function according to claim 1, wherein: the first disinfection filtering device (1424) is arranged at the upper air duct air outlet (1422) and/or the lower air duct air outlet (1423).

6. The PCR amplification operation cabinet with sample transmission function according to claim 1, wherein: PCR amplification module (2) is still including setting up second top wind cavity (22) between PCR amplification (21) top, between PCR amplification (21) be provided with PCR amplification operation window (212) in the front and with PCR amplification operation window (212) corresponding PCR amplification operation window door (213) that sets up, between PCR amplification (21) is provided with second air intake (211) in the side, the outside of operation cabinet is passed through second air intake (211) with the inside intercommunication of PCR amplification between (21), second air intake (211) are provided with second disinfection filter equipment (2111) inside, second top wind cavity (22) be provided with below with lower air intake (223) of PCR amplification between (21) inside intercommunication, second top wind cavity (22) are provided with second updraft ventilator (221) and third disinfection filter equipment (222) inside, second updraft ventilator (221) include second air intake and second air outlet (2211), second top wind cavity (22) pass through second air intake (221) and second air exhaust ventilator (2211) outside updraft ventilator communicates.

7. The PCR amplification cabinet with sample transmission function according to claim 1, wherein: the PCR amplification chamber (21) is internally provided with a storage plate (214) for placing a PCR instrument.

8. The PCR amplification operation cabinet with sample transmission function according to claim 1, wherein: still including removing support body (4), remove support body (4) and be used for supporting sample preparation module (1) with PCR amplification module (2), remove support body (4) and set up sample preparation module (1) with the below of PCR amplification module (2), it is provided with removal gyro wheel (41) in the below to remove support body (4).

9. The PCR amplification operation cabinet with sample transmission function according to claim 1, wherein: the transmission air suction opening (315) is positioned at the lower edge of the inner rear side of the sample transmission room (31), and the transmission air supplement opening (316) is positioned at the upper edge of the inner rear side of the sample transmission room (31).

10. The PCR amplification cabinet with sample transmission function according to claim 1, wherein: the sample preparation room (11), the PCR amplification room (21) and the sample transmission room (31) are internally provided with air pressure sensors.

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