CN110479388A - A kind of sterile operating device for stem cell preparation - Google Patents
A kind of sterile operating device for stem cell preparation Download PDFInfo
- Publication number
- CN110479388A CN110479388A CN201910771499.9A CN201910771499A CN110479388A CN 110479388 A CN110479388 A CN 110479388A CN 201910771499 A CN201910771499 A CN 201910771499A CN 110479388 A CN110479388 A CN 110479388A
- Authority
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- China
- Prior art keywords
- sterile
- operating
- stem cell
- cell preparation
- cabinet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 24
- 230000001954 sterilising effect Effects 0.000 claims abstract description 33
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 30
- 238000012546 transfer Methods 0.000 claims abstract description 17
- 239000000126 substance Substances 0.000 claims abstract description 13
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 27
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 7
- 238000002955 isolation Methods 0.000 claims description 6
- 238000002474 experimental method Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 6
- 238000011169 microbiological contamination Methods 0.000 abstract description 3
- 238000002834 transmittance Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 description 7
- 239000003814 drug Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
- A61L2/202—Ozone
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
- B01L1/04—Dust-free rooms or enclosures
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of sterile operating device for stem cell preparation, comprising: cabinet, the cabinet include at least three operating spaces, and three operating spaces are independent of one another, and are connected by abacterial transferring device；Chemical sterilization device, the chemical sterilization device are connected with operating space；Physical sterilization device, the physical sterilization device are connected with cabinet.Sterile operating device for stem cell preparation of the invention, pass through being used cooperatively for chemical sterilization device and physical sterilization device, it can effectively realize seamless sterilization, the setting of abacterial transferring device, can again stem cell preparation multiple links in, it realizes sterile transfer, avoids with the failure of an experiment caused by microbiological contamination in transmittance process.
Description
Technical field
The present invention relates to biological medicine apparatus fields, specifically, are related to a kind of sterile working for stem cell preparation
Device.
Background technique
Aseptic operating platform is a kind of necessaries of biological medicine research field, it can be the experimental implementation of scientific research personnel
One sterile clean room is provided.In general, needing to carry out ultraviolet go out to microbial sterility station before experiment starts
Bacterium, but since light is along straightline propagation, so if in aseptic operating platform there are ultraviolet lamp irradiate less than dead angle, at dead angle
Easy harbor bacteria；The operating process of biological medicine experiment needs the space of relative closure, avoids microbiological contamination；But it is prepared in stem cell
Process, operation between different preparation steps is there are the demand that trial target shift, normal because can not during this transfer step
Guarantee aseptic condition and causes the failure of an experiment.
Summary of the invention
The present invention provides a kind of aseptic and is able to achieve the sterile behaviour for stem cell preparation of experiment product sterile transfer
Make device.
The technical scheme to solve the above technical problems is that
The present invention provides a kind of sterile operating device for stem cell preparation, comprising:
Cabinet, the cabinet include at least three operating spaces, and three operating spaces are independent of one another, and pass through sterile transfer
Device connection；
Chemical sterilization device, the chemical sterilization device are connected with operating space；
Physical sterilization device, the physical sterilization device are connected with cabinet.
The beneficial effects of the present invention are: pass through being used cooperatively for chemical sterilization device and physical sterilization device, Neng Gouyou
The realization seamless sterilization of effect, the setting of abacterial transferring device, can again stem cell preparation multiple links in, realize sterile biography
It passs, avoids with the failure of an experiment caused by microbiological contamination in transmittance process.
Further, the operating space is is surrounded by station and the wall interior panel that is connected to above station and front apron
Cavity, the front apron are equipped with cabinet body front window and are provided with transparent isolation plate, be arranged on the transparent isolation plate every bacterium manipulator
Set.
Further, the wall interior panel is equipped at least one sliding door, and the sliding door is equipped with door handle, the push-and-pull
Door is tightly connected with wall interior panel.
Further, the chemical sterilization device includes ozone generating-device or acetic acid generating device and circulating fan, institute
It states ozone generating-device or acetic acid generating device is connected with cabinet, the circulating fan is connected with cabinet, the ozone
Generating device or acetic acid generating device are connected with operating space.
Further, the operating space is equipped with sterilizer connecting hole, and the outlet of the circulating fan is gone out by described
Bacterium device connecting hole is connected with operating space.
Further, it is equipped between the circulating fan and operating space and flows film.
Further, filter device is equipped at the air outlet of the circulating fan.
Further, physical sterilization device includes ultraviolet lamp.
Further, further include air exhausting device and return air inlet, the air exhausting device is exhaust fan, the exhaust fan into
Air port is connected with operating space；The lower part of operating space is arranged in the return air inlet, is equipped at the air inlet and return air inlet
Filter.
Further, the abacterial transferring device includes:
Sterile Transfer pipe, the sterile Transfer pipe are equipped at least three openings for being connected with operating space；
Sterile conveyer belt, the sterile conveyer belt are arranged in sterile Transfer pipe, the sterile conveyer belt and motor phase
Connection；
Sterile air port, the sterile air port are two both ends that sterile Transfer pipe is arranged in, one of them sterile air port
It is connected with exhaust blower.
Structure of the invention is compact, reduces 30% in contrast to Biohazard Safety Equipment occupied area；All production processes are all in nothing
It is completed inside bacterium operating device, the environment carried out outside cabinet body must be passed through similar to Biohazard Safety Equipment transmitting by reducing, it is ensured that all
Production procedure is all completed inside sterile operating device, and purification security level is high, and cross contamination link is few, has further ensured production
The preparation of product purifies security context；It is and outer simultaneously as all production links are completed in closed sterile operating device
Boundary does not contact directly, completes inside sterile operating device as long as production is cleared out a gathering place, the efficiency cleared out a gathering place of significant increase, into
One step reduces the cost cleared out a gathering place, it is ensured that the completeness and integrality cleared out a gathering place.
Detailed description of the invention
Fig. 1 is backsight structural representation of the invention；
Fig. 2 is side structure schematic view of the invention；
The structural schematic diagram of the position Fig. 3 sterile transmission device of the invention.
Detailed description of the invention:
1. cabinet, 2. air inlets, 3. pointer differential pressure indicators, 4. power switches, 5. light switches, 6. control panels, 7. follow
Ring blower, 8. filters, 9. headlamps, 10. sliding doors, 11. knob, 12. return air inlets, 13. flow film, 14. return air cabinets,
15. sterile transmission device, 16. exhaust fans, 17. exhaust-air filters, 18. integrated pass-through boxes, 19. sterilizer connecting holes, 20. thoroughly
Bright partition.
Specific embodiment
The principle and features of the present invention will be described below with reference to the accompanying drawings, and the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the invention.
The present invention provides a kind of sterile operating device for stem cell preparation, comprising:
Cabinet 1, the cabinet include at least three operating spaces, and three operating spaces are independent of one another, and pass through sterile biography
Delivery device connection；Chemical sterilization device, the chemical sterilization device are connected with operating space；Physical sterilization device, the physics
Bactericidal unit is connected with cabinet.
The operating space is the chamber that is surrounded by station and the wall interior panel 10 that is connected to above station and front apron 11
Body, the front apron 11 are equipped with cabinet body front window and are provided with transparent isolation plate 20, and setting is grasped every bacterium on the transparent isolation plate 20
Make gloves.
As further scheme of the invention, the wall interior panel 10 is equipped at least one sliding door, on the sliding door
Equipped with door handle, the sliding door and wall interior panel are tightly connected.
As further scheme of the invention, the chemical sterilization device includes that ozone generating-device or acetic acid fill
It sets and is connected with circulating fan 7, the ozone generating-device or acetic acid generating device with cabinet, the circulating fan 7 and case
Body 1 is connected, and the ozone generating-device or acetic acid generating device are connected with operating space.
As further scheme of the invention, the operating space is equipped with sterilizer connecting hole 19, the circulated air
The outlet of machine 7 is connected by the sterilizer connecting hole 19 with operating space.
As further scheme of the invention, it is equipped between the circulating fan and operating space and flows film 13.
As further scheme of the invention, filter device 8 is equipped at the air outlet of the circulating fan.
As further scheme of the invention, physical sterilization device includes ultraviolet lamp.
It further include air exhausting device and return air inlet 12 as further scheme of the invention, the air exhausting device is air draft wind
The air inlet 2 of machine 16, the exhaust fan 16 is connected with operating space；The lower part of operating space, institute is arranged in the return air inlet 12
It states and is equipped with filter at air inlet 12 and return air inlet 2, can be as shown in Figure 2 the exhaust-air filter at setting exhaust fan
17。
Pointer differential pressure indicator 3, power switch 5, light switch 5 and control panel 6, the finger are additionally provided on the cabinet 1
Pin type differential pressure indicator 3, power switch 5, light switch 5 and control panel 6 are arranged at cabinet top, and are connected with wall box
It connects.For the pressure in display operation area, the power switch is controlled to a power supply for controlling electricity the pointer-type differential pressure indicator
Source；The light switch 5 is connected with the headlamp 9 being arranged in operating space, for controlling the on and off of headlamp.
As further scheme of the invention, the abacterial transferring device 15 includes:
Sterile Transfer pipe 151, the sterile Transfer pipe 151 are equipped at least three for being connected with operating space
Opening 152；
Sterile conveyer belt 153, the sterile conveyer belt 153 are arranged in sterile Transfer pipe 151, the sterile conveyer belt
It is connected to the motor and connects；
Sterile air port, the sterile air port are two both ends that sterile Transfer pipe 151 is arranged in, one of sterile wind
Mouth is connected with exhaust fan 16.
As further scheme of the invention, openable and closable sliding door can be equipped at the opening 152.
As further scheme of the invention, above-mentioned filter device and filter can use HVAC filter.
Sterile operating device for stem cell preparation of the invention is mainly by stainless steel frame, stainless steel station, wind
Machine, HVAC filter, PLC control system, sterile transfer system and disinfection system are constituted.It is mainly used for mentioning when the preparation of various cells
Meet legal requirement for one, while can be to the A grade sterile laminar flow environment that sample, environment, personnel are protected.In use,
Equipment purifies B grades of clean airs of environment to send after A grade standard to station, provides gnotobasis, meanwhile, systematic air flow is designed as
Negative pressure, station have glass isolating device, prevent operating space air excessive, pollute to environment and operator.
The present invention, which is used in, to be placed in B grades of toilets, provides the laminar flow environment of an integral asepsis for production stem cell.
The invention also includes PLC control system, the circulating fan, exhaust fan, headlamp, sterilamp are controlled by PLC
System control processed can carry out automation control in control panel, and Control System Design has operation mode and sterilizing mode.
Equipment operation platform needs the equipment used by stem cell manipulation, fully considers each equipment to laminar flow and cleanliness
It influences, subregion setting, and is separated by acrylic glass.
Operation mode: when the normally-open operation mode of equipment, ultraviolet lamp is closed, station front glass panel rises 20cm,
From equipment lower part air inlet successively through primary filter, blower, HVAV, forming layer is streamed to station region environment fresh air.Behaviour
It is partially additionally provided with return air cabinet 14 after making platform, return air cabinet 14 is connected with operating space, and the laminar-flow air for flowing through station passes through back
Wind deflector hole enters air duct to return air cabinet 14, forms air quantity circulation.Exhaust fan and high efficiency particulate air filter are equipped at the top of equipment, it is raw
It is opened when production, 30% used air quantity is discharged.
Sterilizing mode: exhaust fan is closed when sterilizing, air inlet is closed, and station front glass panel is closed, ozone or second
Acid generator is opened, and circulating fan opens low air quantity mode and carries out microcirculation, sterilization material is sent each to station and air duct
Corner, exhaust fan, air inlet are opened after sterilizing, are aerated, sterilization material is discharged.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of sterile operating device for stem cell preparation characterized by comprising
Cabinet, the cabinet include at least three operating spaces, and three operating spaces are independent of one another, and pass through abacterial transferring device
Connection；
Chemical sterilization device, the chemical sterilization device are connected with operating space；
Physical sterilization device, the physical sterilization device are connected with cabinet.
2. the sterile operating device according to claim 1 for stem cell preparation, which is characterized in that the operating space is
The cavity surrounded by station and the wall interior panel being connected to above station and front apron, before the front apron is equipped with cabinet body
Window is provided with transparent isolation plate, is arranged on the transparent isolation plate every bacterium operating gloves.
3. the sterile operating device according to claim 2 for stem cell preparation, which is characterized in that on the wall interior panel
Equipped at least one sliding door, the sliding door is equipped with door handle, and the sliding door and wall interior panel are tightly connected.
4. the sterile operating device according to claim 1 for stem cell preparation, which is characterized in that the chemical sterilization
Device includes that perhaps acetic acid generating device and the circulating fan ozone generating-device or acetic acid fill ozone generating-device
It sets and is connected with cabinet, the circulating fan is connected with cabinet, the ozone generating-device or acetic acid generating device and behaviour
It is connected as area.
5. the sterile operating device according to claim 4 for stem cell preparation, which is characterized in that on the operating space
Equipped with sterilizer connecting hole, the outlet of the circulating fan is connected by the sterilizer connecting hole with operating space.
6. the sterile operating device according to claim 4 for stem cell preparation, which is characterized in that the circulating fan
It is equipped between operating space and flows film.
7. according to any sterile operating device for stem cell preparation of claim 4-6, which is characterized in that described to follow
Filter device is equipped at the air outlet of ring blower.
8. the sterile operating device according to claim 1 for stem cell preparation, which is characterized in that physical sterilization device
Including ultraviolet lamp.
9. the sterile operating device according to claim 1 for stem cell preparation, which is characterized in that further include air draft dress
It sets and return air inlet, the air exhausting device is exhaust fan, the air inlet of the exhaust fan is connected with operating space；The return air
The lower part of operating space is arranged in mouth, is equipped with filter at the air inlet and return air inlet.
10. -9 any sterile operating device for stem cell preparation according to claim 1, which is characterized in that described
Abacterial transferring device includes:
Sterile Transfer pipe, the sterile Transfer pipe are equipped at least three openings for being connected with operating space；
Sterile conveyer belt, the sterile conveyer belt are arranged in sterile Transfer pipe, and the sterile conveyer belt, which is connected to the motor, to be connect；
Sterile air port, the sterile air port are two both ends that sterile Transfer pipe is arranged in, one of them sterile air port and row
Blower is connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910771499.9A CN110479388B (en) | 2019-08-21 | 2019-08-21 | Sterile operation device for stem cell preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910771499.9A CN110479388B (en) | 2019-08-21 | 2019-08-21 | Sterile operation device for stem cell preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110479388A true CN110479388A (en) | 2019-11-22 |
| CN110479388B CN110479388B (en) | 2024-05-17 |
Family
ID=68552393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910771499.9A Active CN110479388B (en) | 2019-08-21 | 2019-08-21 | Sterile operation device for stem cell preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110479388B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111485739A (en) * | 2020-04-15 | 2020-08-04 | 江苏超净电子系统工程有限公司 | Cleaning level crossing protection device installed in modularized mode |
| CN113181979A (en) * | 2021-04-26 | 2021-07-30 | 苏州苏因智启生物科技有限公司 | Biological sample processing environment retention system |
| CN113462563A (en) * | 2021-06-24 | 2021-10-01 | 冰山松洋生物科技(大连)有限公司 | Sterile transfer system based on cell workstation and collection room UNICOM |
| IT202000009868A1 (en) * | 2020-05-05 | 2021-11-05 | Gilardoni Spa | SANITATION METHOD AND APPARATUS FOR THE KILLING OF THE VIRAL AND BACTERIAL LOAD PRESENT ON OBJECTS. |
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| EP2133101A1 (en) * | 2007-03-28 | 2009-12-16 | Sanyo Electric Co., Ltd. | System for maintaining sterile environment |
| CN102500434A (en) * | 2011-11-08 | 2012-06-20 | 上海东富龙科技股份有限公司 | Isolator for sterility detection process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111485739A (en) * | 2020-04-15 | 2020-08-04 | 江苏超净电子系统工程有限公司 | Cleaning level crossing protection device installed in modularized mode |
| IT202000009868A1 (en) * | 2020-05-05 | 2021-11-05 | Gilardoni Spa | SANITATION METHOD AND APPARATUS FOR THE KILLING OF THE VIRAL AND BACTERIAL LOAD PRESENT ON OBJECTS. |
| CN113181979A (en) * | 2021-04-26 | 2021-07-30 | 苏州苏因智启生物科技有限公司 | Biological sample processing environment retention system |
| CN113462563A (en) * | 2021-06-24 | 2021-10-01 | 冰山松洋生物科技(大连)有限公司 | Sterile transfer system based on cell workstation and collection room UNICOM |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110479388B (en) | 2024-05-17 |
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