CN113181979A - Biological sample processing environment retention system - Google Patents

Biological sample processing environment retention system Download PDF

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Publication number
CN113181979A
CN113181979A CN202110454181.5A CN202110454181A CN113181979A CN 113181979 A CN113181979 A CN 113181979A CN 202110454181 A CN202110454181 A CN 202110454181A CN 113181979 A CN113181979 A CN 113181979A
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China
Prior art keywords
space
operation space
operating
disposed
sample
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Granted
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CN202110454181.5A
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Chinese (zh)
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CN113181979B (en
Inventor
杨功达
曾丰波
胡秀弟
李凤梅
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Suzhou Lansha Biotechnology Co ltd
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Suzhou Sunzhiqi Biotechnology Co ltd
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Priority to CN202110454181.5A priority Critical patent/CN113181979B/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00Enclosures; Chambers
    • B01L1/04Dust-free rooms or enclosures
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00Enclosures; Chambers
    • B01L1/50Enclosures; Chambers for storing hazardous materials in the laboratory, e.g. cupboards, waste containers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors
    • B01L2200/141Preventing contamination, tampering
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

In the biological sample processing environment maintaining system provided by the invention, the first operation space and the second operation space are arranged at intervals, the first operation space and the second operation space are in a micro-positive pressure state, and the air pressure in the second operating space is greater than or equal to the air pressure in the first operating space, and the operating area is arranged at the edge of the second operating space and is far away from the waste pipeline, so that the risk of pollution caused by the air in the first operating space to the second operating space is reduced, and on the second hand, a third space for accommodating waste is arranged below the second operation space and is connected through a waste pipeline, so that the waste is collected quickly and intensively, the risk of pollution of the waste to a sample is reduced, and in the third aspect, a quiet zone or a zone with less air flow is formed at the edge area of the second operation space, and the operation zones are arranged at the positions, so that the influence of the air flow on the operation is reduced, and the risk of polluting the sample is reduced.

Description

Biological sample processing environment retention system
Technical Field
The present invention relates to a biological sample processing environment retention system.
Background
Carry out preliminary treatment to biological sample, sort, in the processing procedure such as detection, need go on in clean environment, at present, set up clean cabin usually, after the clean cabin is sent into to the sample, handle biological sample through operating means, be provided with the waste collection box simultaneously in clean cabin, be used for if, waste such as pipettor suction head is collected, but under this kind of mode, waste collection needs occupation space on the one hand, the while discarded object probably causes the influence to clean environment, and simultaneously, arrange the appearance of advancing in single clean space, operation and waste holding, also cause mutual interference easily or to the problem such as sample pollution.
Therefore, there is a strong need to develop and implement a new alternative solution to solve the above technical problems.
Disclosure of Invention
To this end, the present invention provides a biological sample processing environment maintenance system to solve the above technical problems.
A biological sample processing environment maintaining system includes a first operation unit and a second operation unit,
the first operation unit comprises a first purification assembly, a first operation space and a sample introduction assembly;
the second operation unit comprises a second purification assembly, a second operation space and a third space which are arranged in an up-and-down isolation manner, and a waste pipeline which is communicated with the second operation space and the third space;
the first purification assembly and the second purification assembly are used for conveying purified air into the first operation space and the second operation space from top to bottom respectively, the first operation space and the second operation space are in a micro-positive pressure state, the air pressure in the second operation space is greater than or equal to the air pressure in the first operation space, the second operation space is isolated from the first operation space, the second operation space further comprises an operation area used for processing a sample, and the operation area is arranged at the edge of the second operation space and is far away from a waste pipeline.
Further, a connecting channel is included between the second operating space and the first operating space for transferring the sample between the first operating space and the second operating space through the connecting channel.
Furthermore, a transfer component is arranged between the second operation space and the first operation space, the transfer component can move between the first operation space and the second operation space, and the transfer component comprises a guide rail and a transfer device arranged on the guide rail.
Furthermore, the second operating space further comprises a cover opening area, a cover opening device is arranged in the cover opening area, the guide rail comprises a first end arranged in the first operating space and a second end arranged in the second operating space, and the second end extends to the cover opening area.
Furthermore, a transfer device is further arranged in the first operation space and used for taking out the sample from the sample feeding assembly and placing the sample on a transfer device, and the transfer device is abutted against the first end of the guide rail.
Furthermore, the second operation space is formed by enclosing a second operation table top and a second shell, the second shell comprises a side plate, the side plate and the connecting channel are oppositely arranged, and the cover opening area is arranged close to the side plate.
Furthermore, the cover opening area is arranged separately from the operation area.
Further, the waste pipeline comprises an inlet and an outlet, wherein the inlet is arranged above the table top of the second operating table.
Furthermore, the distance between the inlet and the second operating platform is H1, the transfer device comprises an upper end face, the distance between the upper end face and the second operating platform is H2, and H1 is not less than H2.
Further, the inlet is arranged right below the second purification component.
Has the advantages that: in the biological sample processing environment maintaining system provided by the invention, the first operation space and the second operation space are arranged at intervals, the first operation space and the second operation space are in a micro-positive pressure state, the air pressure in the second operation space is greater than or equal to the air pressure in the first operation space, the operation area is arranged at the edge of the second operation space and is far away from the waste pipeline, on one hand, the air in the first operation space is isolated from the air in the second operation space, the risk of pollution caused by the air in the first operation space to the second operation space is reduced, on the second hand, a third space for accommodating waste is arranged below the second operation space and is connected through the waste pipeline, the rapid centralized collection of the waste is realized, the risk of pollution caused by the waste to a sample is reduced, on the third hand, a calm zone or a zone with less air flow is formed in the edge zone of the second operation space, arranging the operating area at these locations reduces the impact of the gas flow on the operation and reduces the risk of contamination of the sample.
Drawings
FIG. 1 is a simplified schematic diagram of a biological sample processing environment maintenance system according to an embodiment of the present invention;
FIG. 2 is a plan view of the worktop of FIG. 1;
FIG. 3 is a perspective view of FIG. 1;
FIG. 4 is a schematic cross-sectional view A-A of FIG. 3;
fig. 5 is a plan view of the worktop of fig. 3.
Description of the illustrated elements:
a first operation unit 10; a first operating space 11; a first operation table 111; a first housing 112; a first purification assembly 12; a sample introduction assembly 13; a transfer device 14;
a second operation unit 20; a second operating space 21; a second operation table 211; a second housing 212; side plates 2121; a spacer 2122; a cap opening region 213; a cap opening device 2130; a transfer assembly 214; a transfer device 2141; a guide rail 2142; a connecting channel 215; an operating area 216; an operating device 2160; a sample outlet assembly 217; a second purge assembly 22; a third space 23; a waste container 231; a waste conduit 24; a waste channel 240;
a third operating unit 30.
Detailed Description
Referring now to fig. 1-5 in combination, wherein fig. 1 shows a simplified schematic diagram of a biological sample processing environment maintenance system of the present invention, fig. 3 shows a schematic perspective view of an embodiment of the present invention, and fig. 2, 4 and 5 show respective block diagrams of corresponding locations, the present application will be further described with reference to these figures.
The invention provides a biological sample processing environment maintaining system, which is used for maintaining a clean environment when a biological sample is subjected to processing processes such as pretreatment, sampling, sorting, detection and the like, wherein the biological sample comprises animal and plant samples and microorganism samples, and can be used for other situations needing the clean environment maintenance.
1. A first operation unit:
the first operation unit 10 includes a first purification assembly 12, a first operation space 11 and a sample introduction assembly 13, wherein the first operation space 11 is defined by a first operation platform 111 and a first housing 112, and is configured to perform a first operation on a sample in the first operation space 11, it can be understood that the housing is closed, so that the formed first operation space 11 is a closed space to isolate the influence of the outside on the first operation space 11, and it can be understood that a door or window structure can be further disposed on the first housing 112, which is not described herein again.
The first purifying assembly 12 is disposed above the first operation space 11, and is configured to deliver purified air into the first operation space 11 from top to bottom, and to enable the first operation space 11 to be in a micro-positive pressure state, where the micro-positive pressure is within a range of about 1 to 2 atmospheres, and more preferably, within a range of 1 to 1.2 atmospheres, in the first operation space 11.
It will be appreciated that the first cleaning assembly 12 includes a filter device and an air blower device for filtering air, and in some other embodiments, the first cleaning assembly 12 may further include a uv sterilization device for radiation sterilization in the first operation space 11 to further maintain a sterile cleaning environment in the first operation space 11, and preferably, the filter device may be an FFU filter.
The sample feeding assembly 13 is used for conveying a sample into the first operating space 11 through the sample feeding assembly 13, when the sample is contained in the sample tube, the sample feeding assembly 13 conveys the sample tube, in this embodiment, the sample feeding assembly 13 is of a drawer structure and comprises a panel and a pull piece, the first shell 112 comprises an opening part, the pull piece can movably pass through the opening part, in addition, the sample feeding assembly 13 comprises a drawing state and a closing state, when the sample feeding assembly 13 is in the drawing state, the sample or the sample tube can be placed on the pull piece, and when the sample feeding assembly 13 is in the closing state, the opening part is sealed by the surface part.
2. A second operation unit:
the second operation unit 20 is spaced apart from the first operation unit 10, and includes a second purification assembly 22, a second operation space 21 and a third space 23 spaced apart from each other up and down, and a waste pipe 24 communicating the second operation space 21 and the third space 23.
The second purifying assembly 22 is disposed above the second operation space 21, and is configured to deliver purified air into the first operation space 11 from top to bottom, and enable the second operation space 21 to be in a micro-positive pressure state, and the structure of the second purifying assembly 22 may be the same as that of the first purifying assembly 12, which is not described herein again.
The second operation space 21 is defined by the second operation platform 211 and the second housing 212, and is configured to perform corresponding operations on the sample in the second operation space 21, it can be understood that the second operation space 21 includes at least one operation area 216 therein, and the operation area 216 is provided with a corresponding operation device 2160 to perform operations on the sample, such as sample suction, liquid adding, extraction, stirring, separation, culturing, warming, detection, etc., and the operation device 2160 may be a corresponding device in the prior art, and will not be described herein again.
It can be understood that the second operation space 21 is isolated from the first operation space 11, and a connection channel 215 is included between the second operation space 21 and the first operation space 11 for transferring the sample between the first operation space 11 and the second operation space 21 through the connection channel 215, specifically, in this embodiment, a transfer component 214 is disposed between the second operation space 21 and the first operation space 11, and the transfer component 214 can move between the first operation space 11 and the second operation space 21, so as to conveniently transfer the sample between the first operation space 11 and the second operation space 21.
The transfer assembly 214 includes a guide rail 2142 and a transfer device 2141 disposed on the guide rail 2142, the transfer device 2141 is movably disposed on the guide rail 2142 and can move along a direction defined by the guide rail 2142, preferably, the guide rail 2142 includes a first end and a second end, the first end is disposed in the first operating space 11, the second end is disposed in the second operating space 21, and more preferably, the guide rail 2142 is disposed along a top surface of the first operating platform 111 and/or a top surface of the second operating platform 211.
Further, when the sample is disposed in the sample tube, the transfer device 2141 further includes a clamp for clamping the sample tube so that the sample tube can stably move from the first operation space 11 to the second operation space 21, in this case, a transfer device 14 is further disposed in the first operation space 11 for taking out the sample tube from the sample injection assembly 13 and placing the sample tube onto the transfer device 2141, and the transfer device 14 abuts against the first end of the guide rail 2142.
Further, the connecting channel 215 is shaped and sized to allow the transfer tool 2141 and the sample tube to pass through, but the smaller the cross-sectional area of the connecting channel 215 is, the better the cross-sectional area is, so that the airflow between the first operating space 11 and the second operating space 21 is less interacted, and the first operating space 11 is prevented from affecting the environment in the second operating space 21, preferably, a partition plate 2122 is included between the first operating space 11 and the second operating space 21, the connecting channel 215 is disposed on the partition plate 2122, in this embodiment, the connecting channel 215 is an opening on the partition plate 2122, and the cross-sectional area of the connecting channel 215 is less than or equal to 30% of the area of the partition plate 2122.
The third space 23 is disposed below the second operation space 21 and spaced from the second operation space 21, a waste pipe 24 is disposed in the middle area of the second operation table 211, and includes a waste passage 240 communicating the second operation space 21 and the third space 23, for directly throwing a pipette tip, for example, into the third space 23 through the waste passage 240, so as to collect waste through the third space 23, on one hand, the waste is conveniently centralized and processed, on the other hand, the waste is disposed in the third space 23 isolated from the second operation space 21, so that the actions of the operating devices 2160 in the second operation space 21 are not affected or the waste is not contaminated into the sample in the second operation space 21, and at the same time, it can be seen that the first operation space 11 and the second operation space 21 are in a closed state and in a positive pressure state, and the third space 23 is communicated with the external atmosphere, so that the air flow in the second operating space 21 will flow outwards through the waste channel 240, i.e. the waste channel 240 becomes an air flow channel, in which case the area far away from the waste channel 240, e.g. the edge area of the second operating space 21, will form a quiet zone or a zone less affected by the air flow, and when the operating area 216 is arranged at these positions, on the one hand, the sample will not be easily disturbed or contaminated, on the other hand, it will not be easily affected by the air flow, and the sample can be operated more stably, and the operating area 216 is far away from the waste channel 240, i.e. the distance between the operating area 216 and the waste channel 240 is greater than or equal to 5cm, although larger distances, e.g. 8cm, 10cm, are also suitable.
Preferably, the air pressure in the second operation space 21 is greater than or equal to the air pressure in the first operation space 11, so that the environment in the second operation space 21 is less affected by the air flow in the first operation space 11, and it can be understood that, because the first operation space 11 is a closed space and is not provided with an air flow outlet, in this embodiment, a part of the air flow in the first operation space 11 enters the second operation space 21 along with the connecting channel 215 and flows out to the outside through the waste channel 240, in this case, the waste channel 240 should be arranged adjacent to the connecting channel 215. It will be appreciated that in this case, the space on the back side of the connecting channel 215 will be less affected by the airflow in the first operating space 11, and in this embodiment, the second housing 212 includes a side plate 2121 on the back side of the connecting channel 215, specifically, the connecting channel 215 is disposed on the right side of the second operating space 21, the side plate 2121 is disposed on the left side of the second operating space 21, and the operating area 216 is disposed adjacent to the side plate 2121.
Further, the second operating space 21 further includes a cap uncovering area 213, a cap uncovering device 2130 is disposed in the cap uncovering area 213, and is used for opening the cap of the sample tube in the cap uncovering area 213 through the cap uncovering device 2130, so as to facilitate subsequent operations on the sample in the uncapped sample tube, the cap uncovering device 2130 is disposed adjacent to the side plate 2121, so as to be unaffected by the airflow or not to affect the uncapped sample by the airflow during uncovering, preferably, the second end of the guide rail 2142 extends to the cap uncovering area 213, so that the sample tube is directly transferred to the cap uncovering area 213 through the transfer assembly 214, and is easily opened by the cap uncovering device 2130, and the cap uncovering device 2130 may be a cap uncovering device 2130 in the prior art, which will not be described herein again.
When the operation area 216 and the uncapping area 213 are provided at the same time, the uncapping area 213 is provided separately from the operation area 216 to reduce mutual influence.
Further, the waste pipe 24 comprises an inlet and an outlet, wherein the inlet is arranged above the top of the second table 211 and is spaced apart from the top of the second table 211 by a distance H1, such that a dead air zone or a small air flow zone is formed in the space between the top and the inlet. Preferably, the inlet is located directly below the second cleaning assembly 22 so that the influence of the airflow on the area around the waste pipe 24 is further reduced.
Further, the transfer device 2141 includes an upper end surface, the distance between the upper end surface and the top surface of the second operating platform 211 is H2, H1 is greater than or equal to H2, and it can be understood that the upper end surface is the highest point of the transfer device 2141 in the vertical direction.
Further, a waste container 231 is disposed in the third space 23 for receiving waste falling from the waste pipe 24.
Further, the second operation unit 20 further includes a sample outlet module 217 for outputting the processed sample to the outside, and the structure of the sample outlet module 217 may be similar to that of the sample inlet module 13, and is not described herein again.
Further, the biological sample processing environment holding system further includes a third operating unit 30, and the third operating unit 30 has the same structure as the second operating unit 20 and is symmetrically disposed at both sides of the first operating unit 10 with respect to the second operating unit 20.
As described above, in the biological sample processing environment maintaining system provided by the present invention, the first operation space 11 and the second operation space 21 are disposed at an interval, the first operation space 11 and the second operation space 21 are in a micro-positive pressure state, and the air pressure in the second operation space 21 is greater than or equal to the air pressure in the first operation space 11, the operation area 216 is disposed in the second operation space 21, and the operation area 216 is disposed at the edge of the second operation space 21 and away from the waste pipe 24, on one hand, since the first operation space 11 is the area where the sample enters, so that the area is a higher pollution risk area, the second operation space 21 is isolated from the first operation space 11, and the air pressure is greater than or equal to the air pressure in the first operation space 11, so that the air in the first operation space 11 is isolated from the air in the second operation space 21, thereby reducing the risk of the air in the first operation space 11 polluting the second operation space 21, in a second aspect, a third space 23 for receiving waste is provided below the second operating space 21 and connected by a waste pipe 24, so as to achieve a rapid concentrated collection of waste and reduce the risk of contamination of the sample by waste, and in a third aspect, a quiet air zone or a zone with less air flow is formed at the edge area of the second operating space 21, and the operating area 216 is arranged at these positions, so as to reduce the influence of air flow on the operation and reduce the risk of contamination of the sample.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (10)

1. A biological sample processing environment retention system, comprising,
the first operation unit comprises a first purification assembly, a first operation space and a sample introduction assembly;
the second operation unit comprises a second purification assembly, a second operation space and a third space which are arranged in an up-and-down isolation manner, and a waste pipeline which is communicated with the second operation space and the third space;
the first purification assembly and the second purification assembly are used for conveying purified air into the first operation space and the second operation space from top to bottom respectively, the first operation space and the second operation space are in a micro-positive pressure state respectively, the air pressure in the second operation space is greater than or equal to the air pressure in the first operation space, the second operation space is isolated from the first operation space, the second operation space further comprises an operation area used for processing a sample, and the operation area is arranged at the edge of the second operation space and is far away from a waste pipeline.
2. The biological sample processing environment retention system of claim 1, comprising a connecting channel between the second operating space and the first operating space for transferring the sample between the first operating space and the second operating space through the connecting channel.
3. The system of claim 2, wherein a transfer assembly is disposed between the second operating space and the first operating space, the transfer assembly being movable between the first operating space and the second operating space, the transfer assembly including a rail and a transfer device disposed on the rail.
4. The biological sample processing environment retention system of claim 3, wherein the second operating space further comprises a decapping area having a decapping device disposed therein, and the rail comprises a first end disposed within the first operating space and a second end disposed within the second operating space, the second end extending into the decapping area.
5. The system of claim 4, wherein the first operating space further comprises a transfer device for removing the sample from the sample assembly and placing the sample on a transfer device, wherein the transfer device abuts the first end of the rail.
6. The biological sample processing environment retention system of claim 4, wherein the second operating volume is defined by a second operating deck and a second housing, the second housing including a side panel, the side panel being disposed opposite the connecting channel, and the uncapped region being disposed adjacent to the side panel.
7. The biological sample processing environment retention system of claim 4, wherein the uncapped region is disposed separately from the manipulated region.
8. The biological sample processing environment retention system of claim 6, wherein the waste conduit includes an inlet and an outlet, wherein the inlet is disposed above the second stage floor.
9. The biological specimen processing environment retention system of claim 8, wherein the access opening is spaced apart from the second station floor by a distance H1, and the transfer member includes an upper end surface spaced apart from the second station floor by a distance H2, H1 ≧ H2.
10. The biological sample processing environment retention system of claim 8, wherein the inlet is disposed directly below the second purification assembly.
CN202110454181.5A 2021-04-26 2021-04-26 Biological sample processing environment retention system Active CN113181979B (en)

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Application Number Priority Date Filing Date Title
CN202110454181.5A CN113181979B (en) 2021-04-26 2021-04-26 Biological sample processing environment retention system

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Application Number Priority Date Filing Date Title
CN202110454181.5A CN113181979B (en) 2021-04-26 2021-04-26 Biological sample processing environment retention system

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CN113181979A true CN113181979A (en) 2021-07-30
CN113181979B CN113181979B (en) 2023-05-26

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105135566A (en) * 2015-09-09 2015-12-09 同济大学 Pollutant obstructing system based on air directional flowing principle and using method of pollutant obstructing system
CN110479388A (en) * 2019-08-21 2019-11-22 和泓(厦门)生物技术有限公司 A kind of sterile operating device for stem cell preparation
CN211133977U (en) * 2019-10-30 2020-07-31 浙江大学医学院附属第四医院 Multifunctional biological safety cabinet
CN212619023U (en) * 2020-07-10 2021-02-26 苏州安泰空气技术有限公司 Purifying equipment with positive and negative pressure capable of being rapidly converted

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105135566A (en) * 2015-09-09 2015-12-09 同济大学 Pollutant obstructing system based on air directional flowing principle and using method of pollutant obstructing system
CN110479388A (en) * 2019-08-21 2019-11-22 和泓(厦门)生物技术有限公司 A kind of sterile operating device for stem cell preparation
CN211133977U (en) * 2019-10-30 2020-07-31 浙江大学医学院附属第四医院 Multifunctional biological safety cabinet
CN212619023U (en) * 2020-07-10 2021-02-26 苏州安泰空气技术有限公司 Purifying equipment with positive and negative pressure capable of being rapidly converted

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