CN101598739B - Full-automatic modular multi-sample intelligent processing system - Google Patents
Full-automatic modular multi-sample intelligent processing system Download PDFInfo
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- CN101598739B CN101598739B CN2009101580925A CN200910158092A CN101598739B CN 101598739 B CN101598739 B CN 101598739B CN 2009101580925 A CN2009101580925 A CN 2009101580925A CN 200910158092 A CN200910158092 A CN 200910158092A CN 101598739 B CN101598739 B CN 101598739B
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Abstract
The invention discloses a full-automatic modular multi-sample intelligent processing system, which comprises a liquid adding and homogenizing module, an automatic vacuuming and filtering module, an automatic nitrogen blowing and concentrating module, an automatic gelatum permeating and purifying module, an automatic slide phase extracting module, an automatic transmitting and heating module and an operation control module. Corresponding processing modules, experimental condition procedures and operation procedures are edited and selected by the operation control module according to the needs of different samples to be processed to carry out corresponding automatic processing. The full-automatic modular multi-sample intelligent processing system can synchronously process four samples, can continuously process fixteen samples, and carry out processing procedures of liquid adding, homogeneity extraction, filtration, gelatum purification, solid phase extraction, nitrogen blowing on the samples. The full-automatic modular food residual sample processing system realizes full automation of processing the samples, effectively improves the sample processing quantity, simplifies the operation process, improves the detection quality and improves the experimental safety.
Description
Technical field
The present invention relates to a kind of full-automatic modular multi-sample intelligent processing system.
Background technology
At present, the automaticity of the sensitivity of modern instrumental analysises such as food inspection, legal medical expert's evaluation, mensuration precision and assay determination is increasingly high, and the sample pre-treatments stage mainly rests on the situation of artificial treatment sample.Because various food substrates are complicated; The pre-treating method of sample is a difficult point in the food analysis process with technology; The sample pretreatment process of consuming time, effort and inefficiency has become " bottleneck " problem of objectionable impurities retention analysis process in the food, has caused many researchers' in the food analysis chemical field long great attention.Sample liquid feeding, homogeneous, extraction, step such as to purify, dry up be the important step of sample pre-treatments, and the problem that solve artificial liquid feeding, homogeneous, extraction, purifies, dries up is that sample pre-treatments saves time, easy, key that efficient is high.
Summary of the invention
To the problem that prior art exists, the object of the present invention is to provide and a kind ofly make liquid feeding in the sample pre-treatments, homogeneous, extraction, gel infiltration purification, SPE, dry up, constant volume realizes the full-automatic modular multi-sample intelligent processing system that automatic lot is finished dealing with.
For realizing above-mentioned purpose; A kind of full-automatic modular multi-sample intelligent processing system of the present invention; Comprise the liquid feeding homogeneous module that is used for sample is carried out liquid feeding, homogenization processing; Be used for sample solution is carried out the automatic vacuum filtering module of filtration treatment; The automatic nitrogen that is used for sample solution is carried out concentration blows concentrated module, be used for to sample solution carry out purified treatment automatic gel infiltration cleaning module, be used for to sample carry out automatic SPE module that SPE handles, be used to transmit sample tube and to the automatic transmission heating module of its heating, control module; According to required,, carry out corresponding processing automatically through controling module editor selected corresponding processing module, experiment condition program and running program to the processing of the pending sample of difference.
Further, said liquid feeding homogeneous module, said automatic vacuum filtering module, said automatic nitrogen blow concentrated module, said automatic gel infiltration cleaning module, said automatic SPE module and said automatic transmission heating module and all are arranged on the general frame.
Further; Said liquid feeding homogeneous module comprises and adds liquid systems, homogeneous system, purging system; Homogeneous system comprises homogenizer and mechanical arm; Homogenizer is arranged on the mechanical arm and can moves along its guide rail with mechanical arm, and solvent adds liquid systems and is used for quantitatively adding solvent to the sample tube of treating homogeneous, and purging system is used to clean remaining impurities on the homogenizer.
Further, said automatic vacuum filtering module comprises seal chamber, vacuum pump; Receive seal, clamping system; After vacuum pump vacuumizes annular seal space, form and filter test tube liquid level pressure differential up and down, filter back solution and receive through receiving seal; Clamping system is used to clamp the catheter that filters the connection of test tube bottom, with stopping of control solution suction filtration.
Further; Said automatic nitrogen blows concentrated module and comprises automatic liquid-level tracking device, nitrogen air supply system; The nitrogen air supply system comes sample solution is concentrated through sweeping nitrogen to the sample liquid top blast; Automatically the liquid level of liquid-level tracking assembly monitor sample solution is with the unlatching of control nitrogen air supply system work with stop.
Further; Said automatic gel infiltration cleaning module comprises infusion pump, syringe pump, transfer valve, auto injection constant volume device, fraction collection test tube, test tube rack, gel permeation chromatographic column; Syringe pump extracts the constant volume solvent and through auto injection constant volume device the solution that has dried up is received test tube liquid feeding constant volume; Syringe pump will add the solution that receives test tube through auto injection constant volume device again and quantitatively extract out then; Be pressed into gel permeation chromatographic column through transfer valve, infusion pump purifies through transfer valve input gel permeation chromatographic column moving phase again by setting program, and purification process finishes; Auto injection constant volume device moves to fraction collection test tube top, and the purification sample collection that gel permeation chromatographic column is flowed out is in the fraction collection test tube.
Further; Said automatic SPE module comprises solid-phase extraction column; Add liquid systems, constant volume system, auto injection constant volume device, kinematic train and compression system; Add liquid systems extraction setting solvent and collected liquid feeding constant volume in the test tube through auto injection constant volume device to drying up solution, the constant volume system completion auto injection constant volume device quantitative draw samples solvent of the sample solvent from collect test tube again adds solid-phase extraction column, in automatic SPE module sample is carried out SPE.
Further, said automatic transmission heating module comprises heating system, shock mitigation system, register pin, kinematic train.Kinematic train realize sample tube up and down and move left and right, to satisfy corresponding station requirement, heating system is used for sample tube is carried out heat treated.
Full-automatic modular multi-sample intelligent processing system of the present invention can be handled 4 simultaneously, handles 16 samples continuously, and sample is carried out liquid feeding, and homogeneous extracts, and filters gel purification, SPE, the sample preparation process that nitrogen dries up.The residual sample disposal system realizes the whole-course automation of sample preparation in the full-automatic modular food, has effectively improved sample preparation quantity, and the flow process that simplifies the operation improves and detects quality and the security that increases experiment.
Description of drawings
Fig. 1 is a structural representation of the present invention;
Fig. 2 is a vertical view of the present invention;
Fig. 3 is a right view of the present invention.
Embodiment
Extremely shown in Figure 3 like Fig. 1; A kind of full-automatic modular multi-sample intelligent processing system of the present invention; Comprise the liquid feeding homogeneous module 4 that is used for sample is carried out liquid feeding, homogenization processing; Be used for sample solution is carried out the automatic vacuum filtering module 5 of filtration treatment; Be used for the automatic nitrogen that sample solution carries out concentration blown concentrate module 2, be used for to sample solution carry out purified treatment automatic gel infiltration cleaning module 7, be used for to sample carry out SPE handle automatic SPE module 8, be used to transmit sample tube and to the automatic transmission heating module 6 of its heating, control module 3; According to required,, carry out corresponding processing automatically through controling module 3 editors selected corresponding processing module, experiment condition program and running program to the processing of the pending sample of difference.
Liquid feeding homogeneous module 4, automatic vacuum filtering module 5, automatic nitrogen blow to concentrate module 2, automatic gel infiltration cleaning module 7, automatic SPE module 8 and transmit heating module 6 automatically and all are arranged on the general frame 1.
Liquid feeding homogeneous module 4 comprises and adds liquid systems, homogeneous system, purging system; Homogeneous system comprises homogenizer and mechanical arm; Homogenizer is arranged on the mechanical arm and can moves along its guide rail with mechanical arm; Solvent adds liquid systems and is used for quantitatively adding solvent to the sample tube of treating homogeneous, and purging system is used to clean remaining impurities on the homogenizer.
Automatic vacuum filtering module 5 comprises seal chamber; Vacuum pump receives seal, clamping system, after vacuum pump vacuumizes annular seal space; Form and filter test tube liquid level pressure differential up and down; Solution receives through receiving seal after filtering, and clamping system is used to clamp the catheter that filters the connection of test tube bottom, with stopping of control solution suction filtration.
Automatic nitrogen blows concentrated module 2 and comprises automatic liquid-level tracking device, nitrogen air supply system; The nitrogen air supply system comes sample solution is concentrated through sweeping nitrogen to the sample liquid top blast; Automatically the liquid level of liquid-level tracking assembly monitor sample solution is with the unlatching of control nitrogen air supply system work with stop.
Automatically gel infiltration cleaning module 7 comprises infusion pump, syringe pump, transfer valve, auto injection constant volume device, fraction collection test tube, test tube rack, gel permeation chromatographic column, this module sequentially to sample infuse, sample introduction, purification, cleaning and recovery.
Automatically SPE module 8 comprises and adds liquid systems, constant volume system, auto injection constant volume device, kinematic train and compression system.
Automatically transmit heating module 6 and comprise heating system, shock mitigation system, register pin, kinematic train.Kinematic train realize sample tube up and down and move left and right, to satisfy corresponding station requirement, heating system is used for sample tube is carried out heat treated.
During the work of a kind of full-automatic modular multi-sample intelligent processing system of the present invention, with pending sample put into test tube, test tube is put on the test tube rack in order.Through the selected corresponding processing module of operating system editor, experiment condition establishing method program and running program bring into operation by operational outfit according to experimental technique.The test tube rack of placing sample tube through after adding liquid systems, homogeneous system sample being carried out liquid feeding, homogeneous, extraction, cleans homogenizer at purging system in liquid feeding homogeneous module 4.The vacuum pump of automatic vacuum filtering module 2 is opened simultaneously; The automatic kinematic train holder that transmits heating module 6 the reception seal and is moved upward, and the seal chamber closure in the automatic vacuum filtering module 5 vacuumizes, and clamping system is opened; Reagent in the sample tube by suction filtration in the reception test tube that receives on the seal; Suction filtration finishes, and vacuum pump cuts out, and clamping system is closed.Automatically the kinematic train holder that transmits heating module 6 the reception seal and is received test tube and moves to automatic nitrogen and blow and concentrate module 3; Heating system is started working; The nitrogen air supply system is opened simultaneously; Automatically the liquid-level tracking device tracking receives solvent liquid level in the reception test tube on the seal, and solvent is carried out nitrogen purging, and solvent dries up in receiving test tube.Automatically transmitting the kinematic train of heating module 6 is asking reception seal and reception test tube to move to automatic gel infiltration cleaning module 4 again; Syringe pump extracts the constant volume solvent through the reception test tube liquid feeding constant volume of auto injection constant volume device to having dried up; Syringe pump will add the solution that receives test tube through auto injection constant volume device again and quantitatively extract out then; Be pressed into gel permeation chromatographic column through transfer valve, infusion pump purifies through transfer valve input gel permeation chromatographic column moving phase again by setting program.Purification process finishes, and auto injection constant volume device moves to fraction collection test tube top, and the purification sample collection that gel permeation chromatographic column is flowed out is in the fraction collection test tube.Fraction collection finishes; Automatically the kinematic train that transmits heating module 6 moves to automatic nitrogen with fraction collection test tube rack and test tube and blows concentrated module; Heating system is started working, and the nitrogen air supply system is opened simultaneously, and the liquid-level tracking device tracking receives sample solvent liquid level in the fraction collection test tube on the seal automatically; Sample solvent is carried out nitrogen purging, and sample solvent dries up in the fraction collection test tube.Automatically transmitting the kinematic train of heating module 6 is asking fraction collection test tube rack and collection test tube to move to automatic SPE module 8 again.Add liquid systems extraction setting solvent and collected liquid feeding constant volume in the test tube through auto injection constant volume device to drying up, the constant volume completion auto injection constant volume device quantitative draw samples solvent of the sample solvent from collect test tube again adds solid-phase extraction column, in automatic SPE module sample is carried out SPE.Control module and accomplish according to the sample that detects, detection method is carried out the method editor to whole device.
The purpose of this invention is to provide a kind of to sample liquid feeding, homogeneous, extraction, gel infiltration purification, SPE, dry up, residual sample disposal system in the full-automatic modular food of step process such as constant volume.It can make liquid feeding in the sample pre-treatments, homogeneous, extraction, gel infiltration purification, SPE, dry up, constant volume realizes that automatic lot finishes dealing with.Improve sample preparation quantity, the flow process that simplifies the operation improves and detects quality and the security that increases experiment.
The present invention is a residual sample disposal system in a kind of full-automatic modular food, the sample preparation of residue detection such as the agricultural and veterinary chemicals that is used for handling samples such as food, feed, Cereals is residual, biotoxin, chemical pollutant.Native system can be handled 4 simultaneously, handles 16 samples continuously, and sample is carried out liquid feeding, and homogeneous extracts, and filters gel purification, SPE, the sample preparation process that nitrogen dries up.The residual sample disposal system realizes the whole-course automation of sample preparation in the full-automatic modular food, improves sample preparation quantity, and the flow process that simplifies the operation improves and detects quality and the security that increases experiment.
Claims (8)
1. full-automatic modular multi-sample intelligent processing system; It is characterized in that; This system comprises the liquid feeding homogeneous module that is used for sample is carried out liquid feeding, homogenization processing; Be used for sample solution is carried out the automatic vacuum filtering module of filtration treatment; The automatic nitrogen that is used for sample solution is carried out concentration blows concentrated module, be used for to sample solution carry out purified treatment automatic gel infiltration cleaning module, be used for to sample carry out automatic SPE module that SPE handles, be used to transmit sample tube and to the automatic transmission heating module of its heating, control module; According to required,, carry out corresponding processing automatically through controling module editor selected corresponding processing module, experiment condition program and running program to the processing of the pending sample of difference.
2. full-automatic modular multi-sample intelligent processing system as claimed in claim 1; It is characterized in that said liquid feeding homogeneous module, said automatic vacuum filtering module, said automatic nitrogen blow concentrated module, said automatic gel infiltration cleaning module, said automatic SPE module and said automatic transmission heating module and all is arranged on the general frame.
3. full-automatic modular multi-sample intelligent processing system as claimed in claim 1; It is characterized in that; Said liquid feeding homogeneous module comprises and adds liquid systems, homogeneous system, purging system, and homogeneous system comprises homogenizer and mechanical arm, and homogenizer is arranged on the mechanical arm and can moves along its guide rail with mechanical arm; Add liquid systems and be used for quantitatively adding solvent to the sample tube of treating homogeneous, purging system is used to clean remaining impurities on the homogenizer.
4. full-automatic modular multi-sample intelligent processing system as claimed in claim 1 is characterized in that, said automatic vacuum filtering module comprises seal chamber; Vacuum pump receives seal, clamping system, after vacuum pump vacuumizes seal chamber; Form and filter test tube liquid level pressure differential up and down; Solution receives through receiving seal after filtering, and clamping system is used to clamp the catheter that filters the connection of test tube bottom, with stopping of control solution suction filtration.
5. full-automatic modular multi-sample intelligent processing system as claimed in claim 1; It is characterized in that; Said automatic nitrogen blows concentrated module and comprises automatic liquid-level tracking device, nitrogen air supply system; The nitrogen air supply system comes sample solution is concentrated through sweeping nitrogen to the sample liquid top blast, and the automatic liquid level of liquid-level tracking assembly monitor sample solution is with the unlatching of control nitrogen air supply system work with stop.
6. full-automatic modular multi-sample intelligent processing system as claimed in claim 1; It is characterized in that; Said automatic gel infiltration cleaning module comprises infusion pump, syringe pump, transfer valve, auto injection constant volume device, fraction collection test tube, test tube rack, gel permeation chromatographic column; Syringe pump extracts the constant volume solvent and through auto injection constant volume device the solution that has dried up is received test tube liquid feeding constant volume, and syringe pump will add the solution that receives test tube through auto injection constant volume device again and quantitatively extract out then, be pressed into gel permeation chromatographic column through transfer valve; Infusion pump purifies through transfer valve input gel permeation chromatographic column moving phase again by setting program; Purification process finishes, and auto injection constant volume device moves to fraction collection test tube top, and the purification sample collection that gel permeation chromatographic column is flowed out is in the fraction collection test tube.
7. full-automatic modular multi-sample intelligent processing system as claimed in claim 1; It is characterized in that; Said automatic SPE module comprises solid-phase extraction column; Add liquid systems, constant volume system, kinematic train and compression system; Add liquid systems and extract setting solvent, add solid-phase extraction column, sample carried out SPE through the quantitative draw samples solvent of the sample solvent of auto injection constant volume device from collect test tube again in automatic SPE module through the solution collection test tube liquid feeding constant volume of auto injection constant volume device to having been dried up.
8. full-automatic modular multi-sample intelligent processing system as claimed in claim 1; It is characterized in that; Said automatic transmission heating module comprises heating system, shock mitigation system, register pin, kinematic train; Kinematic train realize sample tube up and down and move left and right, to satisfy corresponding station requirement, heating system is used for sample tube is carried out heat treated.
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| CN2009101580925A CN101598739B (en) | 2009-07-20 | 2009-07-20 | Full-automatic modular multi-sample intelligent processing system |
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| CN2009101580925A CN101598739B (en) | 2009-07-20 | 2009-07-20 | Full-automatic modular multi-sample intelligent processing system |
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| CN101598739B true CN101598739B (en) | 2012-05-16 |
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