CN217377902U - Full-automatic nucleic acid extraction amplification analyzer - Google Patents
Full-automatic nucleic acid extraction amplification analyzer Download PDFInfo
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- CN217377902U CN217377902U CN202220273731.3U CN202220273731U CN217377902U CN 217377902 U CN217377902 U CN 217377902U CN 202220273731 U CN202220273731 U CN 202220273731U CN 217377902 U CN217377902 U CN 217377902U
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Abstract
The utility model provides a full-automatic nucleic acid extraction amplification analyzer, which comprises a nucleic acid extraction module, wherein a plurality of reagent boxes are arranged in the nucleic acid extraction module; the amplification detection module can amplify and detect the reaction liquid in the PCR tube; a Tip head loading module in which a plurality of Tip heads are loaded; the cover module can cover the PCR tube in the cover module; the motion driving module is used for driving the liquid transfer pump and the electric clamping jaw to generate horizontal transverse, horizontal longitudinal and vertical displacements, wherein the electric clamping jaw can realize the transfer of the PCR tube among the nucleic acid extraction module, the cover module and the amplification detection module, and can open and tightly cover the cover body covered on the sample tube; the Tip head can be inserted and taken by driving the liquid transfer pump, and liquid transfer among the sample tube, the PCR tube and the reagent hole is realized. The utility model discloses, whole process is whole to be accomplished in the inside automation of machine, need not artifical the participation, and the nucleic acid sample is avoided at the outside transfer of instrument, effectively prevents cross contamination, reduces the cost of labor.
Description
Technical Field
The utility model belongs to the technical field of external diagnosis apparatus design, concretely relates to full-automatic nucleic acid draws amplification analysis appearance.
Background
Nucleic acid extraction and PCR amplification (i.e. Polymerase Chain Reaction, which is a molecular biology technology for amplifying and amplifying specific DNA fragments, can be regarded as special DNA replication outside organisms, and the greatest characteristic of PCR is that a trace amount of DNA can be greatly increased) are important experimental means for molecular biology research.
The existing full-automatic nucleic acid extraction amplification analyzer has low automation degree, needs a large amount of manual participation or has small flux, and is difficult to meet the requirements of the current market.
SUMMERY OF THE UTILITY MODEL
Therefore, the to-be-solved technical problem of the utility model lies in providing a full-automatic nucleic acid extraction amplification analyzer, whole extraction, amplification and testing process are whole to be accomplished in the inside automation of machine, need not artifical the participation, and the nucleic acid sample avoids at the outside transfer of instrument, effectively prevents cross contamination, reduces the cost of labor.
In order to solve the above problem, the utility model provides a full-automatic nucleic acid extraction amplification analyzer, including the instrument base, be provided with on it:
the nucleic acid extraction module is internally provided with a plurality of reagent boxes, and each reagent box is integrated with a sample tube, a PCR tube and a plurality of reagent holes;
the amplification detection module can amplify and detect the reaction liquid in the PCR tube; a Tip head loading module in which a plurality of Tip heads are loaded;
the cover module can cover the PCR tube in the cover module;
the motion driving module is used for driving the liquid transfer pump and the electric clamping jaw to generate horizontal transverse, horizontal longitudinal and vertical displacements, wherein the electric clamping jaw can realize the transfer of the PCR tube among the nucleic acid extraction module, the cover module and the amplification detection module, and can open and close a cover body covered on the sample tube; the drive liquid transfer pump can insert the Tip head and transfer liquid among the sample tube, the PCR tube and the reagent hole.
In some embodiments of the present invention, the substrate is,
the Tip head loading module further comprises a loading frame, a plurality of hole sites are arranged on the loading frame, and one Tip head is arranged on each hole site.
In some embodiments of the present invention, the substrate is,
the motion driving module includes:
a traverse driving assembly;
the longitudinal movement driving assembly is connected with a first vertical movement assembly and a second vertical movement assembly, the first vertical movement assembly is connected with the liquid transfer pump, and the second vertical movement assembly is connected with the electric clamping jaw;
the transverse moving driving assembly can drive the longitudinal moving driving assembly to drive the first vertical moving assembly, the second vertical moving assembly, the liquid transfer pump and the electric clamping jaw to transversely move together.
In some embodiments of the present invention, the substrate is,
the transverse moving driving assembly comprises a first transverse supporting plate connected to the instrument base, a first driving motor and a first synchronous belt wheel are connected to the first transverse supporting plate, a first synchronous belt is connected between a rotating shaft of the first driving motor and the first synchronous belt wheel in a tensioning mode, and the longitudinal moving driving assembly is fixedly connected with the first synchronous belt.
In some embodiments of the present invention, the substrate is,
the longitudinal movement driving assembly comprises a longitudinal supporting plate, a second driving motor and a second synchronous belt wheel are connected to the longitudinal supporting plate, a second synchronous belt is connected between a rotating shaft of the second driving motor and the second synchronous belt wheel in a tensioning mode, one end of the longitudinal supporting plate is fixedly connected onto the first synchronous belt, the other end of the longitudinal supporting plate is slidably supported onto a second transverse supporting plate, and the second transverse supporting plate is connected onto the instrument base and is arranged in parallel with the first transverse supporting plate.
In some embodiments of the present invention, the substrate is,
the first vertical moving assembly comprises a first lead screw motor and a first screw in threaded connection with a lead screw of the first lead screw motor, and the liquid moving pump is connected to the first screw.
In some embodiments of the present invention, the substrate is,
the second vertical moving assembly comprises a second screw rod motor and a second screw nut in threaded connection with a screw rod of the second screw rod motor, and the electric clamping jaw is connected to the second screw nut.
In some embodiments of the present invention, the substrate is,
the instrument base is sleeved with a shell so as to cover the nucleic acid extraction module, the amplification detection module, the Tip head loading module, the cover module and the motion driving module in the shell.
In some embodiments of the present invention, the substrate is,
the waste material chute is arranged in the shell in an inclined mode from high to low, and a Tip head inserted and taken by a liquid transfer gun head of the liquid transfer pump can be sent into a waste storage container outside the shell through the waste material chute after the Tip head is used.
The utility model provides a pair of full-automatic nucleic acid draws amplification analysis appearance, will nucleic acid draws module, Tip head and loads module and amplification detection module integration in an organic whole, utilizes motion drive module drives move liquid pump and electronic clamping jaw and produce horizontal, vertical and vertical displacement, realize moving the liquid between each module and carrying and the separation of material, whole drawing, amplification and testing process are whole to be accomplished in the inside automation of machine, need not artifical the participation, and the nucleic acid sample is avoided at the outside transfer of instrument, effectively prevents cross contamination, reduces the cost of labor.
Drawings
FIG. 1 is a schematic perspective view of a fully automatic nucleic acid extraction and amplification analyzer according to an embodiment of the present invention (with the housing omitted);
FIG. 2 is a top view of the fully automated nucleic acid extraction amplification analyzer of FIG. 1;
FIG. 3 is an assembled perspective view of the electric clamp jaw and the pipetting pump of FIG. 1 and the corresponding vertical displacement assembly.
The reference numerals are represented as:
1. a nucleic acid extraction module; 11. a kit; 2. a Tip head loading module; 3. a longitudinal movement driving component; 31. a longitudinal support plate; 32. a second timing pulley; 33. a second synchronous belt; 4. a liquid transferring pump; 41. a first vertically moving component; 5. a cover module; 6. an electric jaw; 61. a second vertically moving component; 7. an amplification detection module; 8. a traverse driving assembly; 81. a first lateral support plate; 82. a rotating shaft; 83. a first synchronization belt; 84. a second lateral support plate; 9. a waste chute; 100. an instrument base.
Detailed Description
Referring to fig. 1 to 3, according to an embodiment of the present invention, there is provided a full-automatic nucleic acid extraction and amplification analyzer, including an instrument base 100 and a housing disposed thereon, wherein a receiving space is formed between the housing and the instrument base 100, and in the receiving space, the instrument base 100 is provided with: the nucleic acid extraction module 1 is internally provided with a plurality of reagent boxes 11, and each reagent box 11 is integrated with a sample tube, a PCR tube and a plurality of reagent holes; the amplification detection module 7 can amplify and detect the reaction liquid in the PCR tube; a Tip head loading module 2 in which a plurality of Tip heads are loaded; a cover module 5 capable of covering the PCR tube therein; the motion driving module is used for driving the liquid transfer pump 4 and the electric clamping jaw 6 to generate horizontal transverse, horizontal longitudinal and vertical displacements, wherein the electric clamping jaw 6 can realize the transfer of the PCR tube among the nucleic acid extraction module 1, the cover module 5 and the amplification detection module 7, and can open and close a cover body covered on the sample tube; the drive pipetting pump 4 can insert the Tip head and realize pipetting among the sample tube, the PCR tube and the reagent well.
In the technical scheme, the nucleic acid extraction module 1, the Tip head loading module 2, the cover module 5 and the amplification detection module 7 are integrated, the motion driving module is utilized to drive the liquid transfer pump 4 and the electric clamping jaw 6 to generate transverse, longitudinal and vertical displacement, so that liquid transfer between the modules and carrying and separation of materials (such as a PCR (polymerase chain reaction) tube, a Tip head and the like) are realized, the whole extraction, amplification and detection processes are automatically completed in a machine without manual participation, a nucleic acid sample is prevented from being transferred outside the instrument, cross contamination is effectively prevented, the labor cost is reduced, and the full-automatic nucleic acid extraction, amplification and detection processes of sample inlet and outlet are realized.
In some embodiments, the Tip loading module 2 further includes a loading frame, the loading frame has a plurality of hole sites, each hole site is provided with one Tip, the number of the tips can be supplemented at any time for use with the reagent kit 11, it can be understood that the housing is configured with a loading port for placing the reagent kit 11 and the loading frame, and the detection throughput of the fully automatic nucleic acid extraction and amplification analyzer can be greatly improved by using a plurality of Tip heads and a plurality of reagent kits 11.
It should be noted that the nucleic acid extraction module 1, the cover module 5, and the amplification detection module 7 may all adopt related specific functional modules in the prior art, and it can be understood that the core component of the nucleic acid extraction module 1 is the corresponding reagent kit 11, and under the action of the suction and release of the liquid-moving pump 4, each reagent and sample liquid are carried out in each reagent hole, PCR tube, and sample tube according to a preset program (prior art).
In some embodiments, the motion driver module comprises: a traverse driving unit 8; the longitudinal movement driving assembly 3 is connected with a first vertical movement assembly 41 and a second vertical movement assembly 61, the first vertical movement assembly 41 is connected with the liquid transferring pump 4, and the second vertical movement assembly 61 is connected with the electric clamping jaw 6; the traverse driving component 8 can drive the longitudinal movement driving component 3 to drive the first vertical moving component 41, the second vertical moving component 61, the liquid-transferring pump 4 and the electric clamping jaw 6 to translate transversely together. In the technical scheme, the transverse moving driving assembly 8, the first vertical moving assembly 41, the second vertical moving assembly 61 and the longitudinal moving driving assembly 3 jointly realize the position adjustment of the liquid moving pump 4 and the electric clamping jaw 6 in the three-dimensional direction so as to ensure the coordination transfer of reagents and materials among all functional modules.
Specifically, the traverse driving assembly 8 includes a first transverse supporting plate 81 connected to the instrument base 100, a first driving motor and a first synchronous pulley are connected to the first transverse supporting plate, a first synchronous belt 83 is connected between a rotating shaft 82 of the first driving motor and the first synchronous pulley in a tensioning manner, the vertical movement driving assembly 3 is fixedly connected to the first synchronous belt 83, and the first synchronous belt 83 drives the vertical movement of the vertical movement driving assembly 3, so that the structure is simpler and more compact. The longitudinal movement driving component 3 comprises a longitudinal supporting plate 31, a second driving motor and a second synchronous pulley 32 are connected to the longitudinal supporting plate, a second synchronous belt 33 is connected between a rotating shaft of the second driving motor and the second synchronous pulley 32 in a tensioning mode, one end of the longitudinal supporting plate 31 is fixedly connected to the first synchronous belt 83, the other end of the longitudinal supporting plate 31 is slidably supported on a second transverse supporting plate 84, the second transverse supporting plate 84 is connected to the instrument base 100 and is arranged in parallel with the first transverse supporting plate 81, and the other end of the longitudinal supporting plate 31 can be sleeved with a roller, so that two ends of the longitudinal supporting plate 31 are reliably supported, and the stability and reliability of the transverse movement of the longitudinal supporting plate 31 are guaranteed.
The first vertical moving assembly 41 comprises a first lead screw motor and a first nut in threaded connection with a lead screw of the first lead screw motor, and the liquid transferring pump 4 is connected to the first nut; the second vertical moving assembly 61 comprises a second lead screw motor and a second nut in threaded connection with a lead screw of the second lead screw motor, the electric clamping jaw 6 is connected to the second nut, and the lead screw of the corresponding lead screw motor is matched with the screw in a threaded manner, so that the rotation of the lead screw motor is converted into the linear motion of the nut, and further the vertical displacement of the corresponding liquid transfer pump 4 and the electric clamping jaw 6 is realized, and the structure is simpler and more compact.
In some embodiments, a waste chute 9 is further arranged in the housing, the waste chute 9 is arranged in an inclined manner from high to low, a Tip head inserted into a pipette Tip of the pipetting pump 4 can be sent into a waste storage container outside the housing through the waste chute 9 after use, and the pipetting pump 4 can have a Tip head withdrawing function.
The liquid transfer pump 4 and the electric chuck 6 may be related functional components in the related art.
The use flow of the full-automatic nucleic acid extraction and amplification analyzer of the present invention is briefly described below with reference to the accompanying drawings:
an operator inserts a kit 11 inserted with a sample to be tested into a kit slot of a nucleic acid extraction module 1 and pushes a Tip head loading module 2 filled with a Tip head into the instrument, after clicking starts, a longitudinal movement driving assembly 3 and a transverse movement driving assembly 8 (hereinafter referred to as a movement module) drive a liquid transfer pump 4 to move above the Tip head loading module 2, a liquid transfer pump vertical driving module (namely a first vertical movement assembly 41) drives the liquid transfer pump 4 to vertically move downwards to insert the Tip head, then the liquid transfer pump vertical driving module drives the liquid transfer pump 4 to move upwards, then the longitudinal movement driving assembly 3, the transverse movement driving assembly 8 drives the liquid transfer pump 4 and an electric clamping jaw 6 to move above a sample tube in the nucleic acid extraction module 1, the electric clamping jaw vertical driving module (namely a second vertical movement assembly 61) drives the electric clamping jaw 6 to move downwards, and the electric clamping jaw 6 clamps a sample tube cover, then the electric clamping jaw 6 rotates and moves upwards under the driving of the electric clamping jaw driving module, the sample tube is uncovered, then the movement module and the liquid-transferring pump vertical driving module drive the liquid-transferring pump to move to the sample tube to absorb a sample, then the movement module and the electric clamping jaw vertical driving module drive the electric clamping jaw 6 to cover the sample tube, then the movement module and the liquid-transferring pump vertical driving module drive the liquid-transferring pump to extract nucleic acid in the reagent box, then the extracted nucleic acid is added into the PCR tube on the reagent box, then the liquid-transferring pump discards the Tip head to the waste chute 9 to slide out of the instrument, then the movement module and the electric clamping jaw vertical driving module drive the electric clamping jaw to take the PCR tube to the cover module 5, the cover module 5 covers the PCR tube, then the electric clamping jaw 6 takes the PCR tube to the amplification detection module 7, and the amplification detection module 7 amplifies and detects the nucleic acid in the PCR tube, then the electric clamping jaw 6 clamps the detected PCR tube back to the original kit 11, the whole process is automatically completed in the machine, manual participation is not needed, and the nucleic acid sample is transferred outside the machine, so that cross contamination is effectively prevented.
It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.
The above description is only exemplary of the present invention and should not be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (9)
1. The utility model provides a full-automatic nucleic acid extraction amplification analysis appearance which characterized in that, includes instrument base (100), is provided with on it:
the nucleic acid extraction module (1) is internally provided with a plurality of reagent boxes (11), and each reagent box (11) is integrated with a sample tube, a PCR tube and a plurality of reagent holes;
the amplification detection module (7) can amplify and detect the reaction liquid in the PCR tube;
a Tip head loading module (2) in which a plurality of Tip heads are loaded;
a cover module (5) capable of covering the PCR tube therein;
the motion driving module is used for driving the liquid transfer pump (4) and the electric clamping jaw (6) to generate horizontal transverse, horizontal longitudinal and vertical displacements, wherein the electric clamping jaw (6) can realize the transfer of the PCR tube among the nucleic acid extraction module (1), the cover module (5) and the amplification detection module (7), and can open and close a cover body covered on the sample tube; the drive liquid transfer pump (4) can insert the Tip head and transfer liquid among the sample tube, the PCR tube and the reagent hole.
2. The automatic nucleic acid extraction and amplification analyzer of claim 1,
the Tip head loading module (2) further comprises a loading frame, a plurality of hole sites are arranged on the loading frame, and one Tip head is arranged on each hole site.
3. The fully automated nucleic acid extraction amplification analyzer of claim 1, wherein the motion driving module comprises:
a traverse drive assembly (8);
the longitudinal movement driving assembly (3) is connected with a first vertical movement assembly (41) and a second vertical movement assembly (61), the first vertical movement assembly (41) is connected with the liquid transfer pump (4), and the second vertical movement assembly (61) is connected with the electric clamping jaw (6);
the transverse moving driving component (8) can drive the longitudinal moving driving component (3) to drive the first vertical moving component (41), the second vertical moving component (61), the liquid transfer pump (4) and the electric clamping jaw (6) to transversely move together.
4. The automatic nucleic acid extraction and amplification analyzer of claim 3,
the transverse moving driving assembly (8) comprises a first transverse supporting plate (81) connected to the instrument base (100), a first driving motor and a first synchronous belt wheel are connected to the first transverse supporting plate, a first synchronous belt (83) is connected between a rotating shaft (82) of the first driving motor and the first synchronous belt wheel in a tensioning mode, and the longitudinal moving driving assembly (3) is fixedly connected with the first synchronous belt (83).
5. The automatic nucleic acid extraction and amplification analyzer of claim 4,
the longitudinal movement driving assembly (3) comprises a longitudinal supporting plate (31), a second driving motor and a second synchronous pulley (32) are connected onto the longitudinal supporting plate, a rotating shaft of the second driving motor and a second synchronous belt (33) are connected between the second synchronous pulley (32) in a tensioning mode, one end of the longitudinal supporting plate (31) is fixedly connected onto the first synchronous belt (83), the other end of the longitudinal supporting plate is slidably supported onto a second transverse supporting plate (84), and the second transverse supporting plate (84) is connected onto the instrument base (100) and is arranged in parallel with the first transverse supporting plate (81).
6. The automatic nucleic acid extraction and amplification analyzer of claim 5,
the first vertical moving assembly (41) comprises a first lead screw motor and a first screw which is in threaded connection with a lead screw of the first lead screw motor, and the liquid transferring pump (4) is connected to the first screw.
7. The automatic nucleic acid extraction and amplification analyzer of claim 5,
the second vertical moving assembly (61) comprises a second screw motor and a second screw in threaded connection with a screw rod of the second screw motor, and the electric clamping jaw (6) is connected to the second screw.
8. The automatic nucleic acid extraction and amplification analyzer of claim 1,
the instrument base (100) is sleeved with a shell so as to cover the nucleic acid extraction module (1), the amplification detection module (7), the Tip head loading module (2), the cover module (5) and the motion driving module in the shell.
9. The automatic nucleic acid extraction and amplification analyzer of claim 8,
the waste material conveying device is characterized in that a waste material sliding groove (9) is further formed in the shell, the waste material sliding groove (9) is formed in a height-to-height inclined mode, and a Tip head inserted and taken by a liquid transfer gun head of the liquid transfer pump (4) can be conveyed into a waste storage container located on the outer side of the shell through the waste material sliding groove (9) after use.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202220273731.3U CN217377902U (en) | 2022-02-10 | 2022-02-10 | Full-automatic nucleic acid extraction amplification analyzer |
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| CN202220273731.3U CN217377902U (en) | 2022-02-10 | 2022-02-10 | Full-automatic nucleic acid extraction amplification analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024055605A1 (en) * | 2022-09-16 | 2024-03-21 | 湖南元景智造科技有限公司 | Molecular detection system and detection method therefor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024055605A1 (en) * | 2022-09-16 | 2024-03-21 | 湖南元景智造科技有限公司 | Molecular detection system and detection method therefor |
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