CN114217085A

CN114217085A - Dry-type fluorescence immunoassay instrument based on realization of multi-channel and automatic detection

Info

Publication number: CN114217085A
Application number: CN202111550010.9A
Authority: CN
Inventors: 唐金鑫; 林�源; 夏星; 蒋明君; 李蒙蒙; 漆海林; 甘正千; 隆坤; 邓志军
Original assignee: Sichuan Xinjian Kangcheng Biological Co ltd
Current assignee: Sichuan Xinjian Kangcheng Biological Co ltd
Priority date: 2021-12-17
Filing date: 2021-12-17
Publication date: 2022-03-22

Abstract

The invention discloses a dry-type fluorescence immunoassay analyzer based on realization of multi-channel and automatic detection. The technical principle applied by the analyzer is combined from the traditional dry chemical technology, immunochromatography technology, optical information, data analysis technology and the like. The method adopts the biomedical technology and the chromatographic technology to analyze and process information of the chromatograph, the spectrum and the biosensor, is applied to immunity and rapid detection, realizes multi-channel and automatic detection, improves the field applicability of the detection, and presents the advantages of automation and simplification.

Description

Dry-type fluorescence immunoassay instrument based on realization of multi-channel and automatic detection

Technical Field

The invention relates to the technical field of immunoassay analyzers, in particular to a dry-type fluorescence immunoassay analyzer based on realization of multi-channel and automatic detection.

Background

In recent years, due to the influence of factors such as environment on human body, new onset of infectious diseases has caused a sudden and constant threat to human health, and spread of infectious diseases such as atypical pneumonia, middle east respiratory syndrome, influenza, ebola, zika virus, yellow fever, rift valley fever and the like has become a global public health problem. With the development of urbanization and the increase of global trade, the spread risk of natural epidemic infectious diseases is aggravated by environmental changes, and the prevention and control of infectious diseases are not slow. Therefore, the method for carrying out instant and rapid detection of infectious diseases has great significance for the prevention and control work of infectious diseases.

Meanwhile, the blood sample of the human body is rapidly detected, so that the diseased or potential diseases of the human body can be effectively and rapidly detected, and the kit has great significance for effectively treating and preventing diseases in the early stage.

The early immune product is a qualitative product, the immune project is a research object, and a colloidal gold mode is adopted. The product of colloidal gold is the star product at this stage. By the combination principle of antigen and antibody, almost all protein molecules can be marked theoretically, and the defects are low sensitivity and low accuracy, and only negative and positive can be judged; after the technology is improved, the color microsphere mark is adopted, compared with colloidal gold, the sensitivity of the color microsphere mark is improved, the repeatability of the detection result is strong, but the sensitivity is limited; with the development of the technology, the fluorescent microsphere marking technology is applied, the fluorescent microsphere marking is stable in light intensity, high in biocompatibility and basically not influenced by changes of external environments, quantitative detection can be achieved, but the requirements on product technology are high, and the difficulty is improved.

POCT products have the advantages of simple and convenient operation, portability, intellectualization and the like, are rapidly developed as in-vitro diagnosis technologies in recent years, and are applied to detection items of multiple disciplines. The device can quickly obtain the detection result, is convenient for patients and reduces the time for the patients to confirm the diagnosis. Meanwhile, POCT products have the function of continuously using and monitoring the state of an illness, for example, a patient with heart disease can quickly know and control the state of the illness through detection of interval time.

The existing equipment needs a large number of complicated manual steps, so that the operation is complicated, the automation cannot be realized, and meanwhile, the detection channel is single.

Disclosure of Invention

The invention aims to overcome the problems in the background technology and provides a dry-type fluorescence immunoassay analyzer based on multi-channel and automatic detection, which is applied to immunity and rapid detection by analyzing and processing information of a chromatograph, a spectrum and a biosensor by adopting a biomedical technology and a chromatographic technology, realizes multi-channel and automatic detection, improves the field applicability of the analyzer and presents the advantages of automation and simplification.

The purpose of the invention is mainly realized by the following technical scheme:

dry-type fluorescence immunoassay appearance based on realize multichannel and automated inspection, which comprises a frame, be provided with the sample module in the frame, shake even module, suction head module, pull out cap module, application of sample module, hatch module, transportation module and reagent card box module, and the transportation module is connected with reagent card box module and incubation module simultaneously, and the application of sample module is located transportation module and removes track top and application of sample module and suction head module connection, pull out cap module and shake even module connection, the sample module with pull out cap module and shake even module connection.

The fluorescence immunoassay (also known as fluorescent antibody technology) is the earliest developed one in the labeled immunoassay, and is a technology established on the basis of immunology, biochemistry and microscope technology, and a dry immunofluorescence analyzer: the sample solution dropped on one end of the membrane is moved to the other end by the capillary action of the membrane (lateral flow based on chromatography), the analyte is combined with a receptor (antigen or antibody) fixed on a certain area on the membrane in the moving process and is subjected to solid phase, irrelevant substances are separated by crossing the area, and then the test result is judged by detecting a marker (qualitative, semi-quantitative and quantitative).

The existing fluorescence immunoassay analyzer needs a large amount of complicated manual steps for auxiliary detection. In order to solve the problem, this scheme is through setting up the sample module in the frame, shake even module, the suction head module, pull out the cap module, the application of sample module, hatch the module, transportation module and reagent card box module, this scheme arranges and connects it as required position, make it satisfy the user demand, adopt biomedical technology, chromatography technology is through carrying out analysis processes to chromatogram, the spectrum, biosensor information, be applied to immunity and short-term test, realize multichannel and automated inspection, improve its on-the-spot suitability, demonstrate the advantage of automation and simplification. The fluorescent microsphere is adopted for labeling and then the optical information technology is combined, so that the rapid detection and reaction of the product are more controllable, the detection sensitivity and the precision are greatly improved, and the method is one of the future technical development directions.

Furthermore, the sample module comprises a sample frame bottom plate, the sample frame bottom plate is fixed with the rack, a sample frame is arranged on the sample frame bottom plate, and the sample frame is positioned below the cap pulling module and the shaking module and movably connected with the cap pulling module and the shaking module; a first driving mechanism is arranged below the bottom plate of the sample frame, the first driving mechanism is connected with a sample feeding shifting fork, the sample feeding shifting fork is connected with the sample frame, and the sample frame and the sample feeding shifting fork can horizontally move relative to the bottom plate of the sample frame under the action of the first driving mechanism; a transverse bottom plate is arranged outside the sample frame bottom plate, a second driving mechanism and a scanner are installed on the transverse bottom plate, the sample frame can be connected with the second driving mechanism after moving under the action of the first driving mechanism, and horizontally moves relative to the sample frame bottom plate under the action of the second driving mechanism and passes through a scanner station; and a third driving mechanism is arranged below the bottom plate of the sample frame, the third driving mechanism is connected with a sample outlet shifting fork, the sample outlet shifting fork can be connected after the sample frame moves along with the second driving mechanism, and the sample frame can horizontally move relative to the bottom plate of the sample frame under the action of the third driving mechanism. This scheme is through advancing kind and going out the appearance and carrying out the subregion, realizes simple circulation in order to improve the quantity that the sample was put into to the single, promotes supporting instrument efficiency. The automatic moving of the sample rack and the automatic reading of the sample information can be realized.

Further, the shaking module comprises a supporting mechanism, the supporting mechanism is fixed with the rack, a shaking main body and a driving mechanism IV are arranged on the supporting mechanism, the driving mechanism IV is fixed with the supporting mechanism, the shaking main body is simultaneously connected with the driving mechanism IV and the supporting mechanism, the shaking main body can rotate around the joint of the supporting mechanism under the driving of the driving mechanism IV, and the sample rack and the cap pulling module are movably connected with the shaking main body; shake even main part below and be provided with clamping mechanism and actuating mechanism five, and clamping mechanism can move the relatively supporting mechanism and realize pressing from both sides tightly or loosen the action under actuating mechanism five drives, and clamping mechanism is including shaking even holder, briquetting and linear guide five, and shake even holder and linear guide five and connect, and linear guide five is fixed on supporting mechanism, and the briquetting is fixed with shaking even holder, and the briquetting with shake elastic connection between the even main part, shake even holder and can move along linear guide five, shake even holder and actuating mechanism five and connect, actuating mechanism five is connected with and presss from both sides tight mounting panel, is provided with the gyro wheel on pressing from both sides tight mounting panel, presss from both sides tight mounting panel and makes the gyro wheel get into the briquetting and shake even holder contact with shaking even holder after between the holder under actuating mechanism five effects linear motion. Most of the existing in vitro diagnostic analyzers still adopt manual operation to complete the stirring and mixing of sample test tubes, and the manual work load is large. Or the single equipment shakes even processing, and the single equipment shakes even processing back and still needs the manual work to move into inside the instrument and detect, has increased manual work volume and latency, can't satisfy present demand to equipment high efficiency operation and automation. Meanwhile, if the stirring and mixing of the sample test tube by the existing in vitro diagnostic analyzer is carried out by shaking up the single device, the cost of the device is additionally increased. In order to solve the problems, the scheme is that through the structure, the driving mechanism drives the shaking main body to rotate in four directions, the shaking main body can be used for installing a plurality of test tubes according to needs, the driving mechanism drives the clamping mechanism to move to clamp or loosen the sample test tubes, the clamping and shaking of the plurality of test tubes can be realized simultaneously, the clamping and loosening of the test tubes can be automatically completed, manual operation can be simulated to realize 90-degree rotation and mixing of the samples, the whole process is automatically completed, and the test tube shaking device is accurate and efficient.

Furthermore, the cap pulling module comprises a transverse plate connecting frame, the transverse plate connecting frame is fixed with the rack, a moving mechanism and a plurality of clamping jaws are mounted on the transverse plate connecting frame, the clamping jaws are connected with the moving mechanism and can move relative to the transverse plate connecting frame under the driving of the moving mechanism, and the clamping jaws are movably connected with the shaking main body and the sample rack; the transverse plate connecting frame is provided with a clamping mechanism, the clamping mechanisms are connected with the clamping jaws, and the clamping jaws are clamped or loosened under the action of the clamping mechanisms; the transverse plate connecting frame is provided with a rotating mechanism, the rotating mechanism is connected with the clamping jaw, and the clamping jaw rotates around the axis of the rotating mechanism. The extraction of most current instruments to reagent sample is handled and is all adopted artifical manual completion basically, and manual operation is loaded down with trivial details, and easily produces secondary pollution, and to the disposable extraction detection of sample test tube, this kind of extraction efficiency is low, and intensity of labour is big, and the disposable extraction detection produces the trouble that detects the anomaly easily and lead to providing reagent sample again simultaneously. Some manufacturers start trying to pull out the cap automatically, the in vitro diagnostic analyzer with the automatic cap pulling function on the market at present has a single function and a complex structure, and reagent samples cannot be extracted and detected for many times and can still be extracted and detected for only one time, so that the device which can simultaneously perform detection on multiple different items and perform cap pulling sampling and cap storage on the reagent samples for many times can realize the function in one instrument, and the device is very necessary. The device can automatically detect the existence of the test tube cap, complete the cap pulling and the cap covering of the test tube, automatically complete the accurate and efficient device in the whole process, can move relative to the transverse plate connecting frame under the driving of the moving mechanism by connecting the clamping jaws with the moving mechanism, can clamp or loosen the clamping jaws under the action of the clamping mechanism, can rotate around the axis of the rotating mechanism by connecting the clamping jaws with the clamping jaws, can simultaneously realize the rotation of the cap pulling and the cap covering of a plurality of test tubes, can automatically detect the existence of the test tube cap, and can finish the cap pulling and the cap covering of the test tube accurately and efficiently.

Furthermore, the suction head module comprises a suction head box mounting plate, the suction head box mounting plate is fixed with the rack, a suction head box is arranged on the suction head box mounting plate, a driving mechanism six is arranged on the suction head box mounting plate, the driving mechanism six is connected with the suction head box, the suction head box can move relative to the suction head box mounting plate under the action of the driving mechanism six, and the suction head box is connected with the sample adding module in the moving process; be provided with actuating mechanism seven on the suction head box mounting panel, actuating mechanism seven is connected with punctures the head, punctures the head and vertically removes under actuating mechanism seven effect. Through this kind of structure, realize that automatic suction head removes to corresponding position to stab the head and realize the rupture of membranes to reagent card strip tectorial membrane, whole process automation goes on, reduces manual operation.

Furthermore, the sample adding module comprises a sample adding stand column, the sample adding stand column is fixed with the rack, a first sample adding transverse plate is arranged between the sample adding stand columns, the first sample adding transverse plate is fixed with the sample adding stand column, a second sample adding transverse plate is arranged between the first sample adding transverse plates, the second sample adding transverse plate can move along the first sample adding transverse plate, a gun can be arranged on the second sample adding transverse plate, the gun can move along the second sample adding transverse plate, and meanwhile the gun can move in the vertical direction and can be inserted into a head suction box or a shaking uniform main body or a sample frame. Because whole application of sample process can involve the multiple spot, so need multidirectional removal, current chemiluminescence immunoassay appearance application of sample process removes complicacy, and hardly remove and target in place, can't accomplish functions such as follow-up suction head automatic addition and removing, sample application of sample and sample stock layout, this scheme carries out horizontal migration through the application of sample diaphragm, the rifle of arranging carries out level and vertical removal, can move to each preset position in the structure, realize functions such as automatic addition of suction head and removing, sample application of sample and sample stock layout.

Further, the incubation module comprises an incubation data acquisition mechanism and a waste card strip removing mechanism, the transportation module can relatively incubate the data acquisition mechanism and remove the waste card strip removing mechanism, the transportation module can be movably connected with the incubation data acquisition mechanism and the waste card strip removing mechanism, a heating plate and a temperature sensor are arranged on the incubation data acquisition mechanism, and the temperature sensor is connected with the heating plate. The process of hatching is not considered alone when realizing hatching in present chemiluminescence immunoassay appearance, all will hatch the subassembly and transport the subassembly together fixedly, just it is less to lead to hatching the position in a storehouse under this kind of structure, influence instrument efficiency of software testing, the interpolation to reagent card strip simultaneously with remove all manual completion, whole process operation is loaded down with trivial details, low efficiency, this scheme is then to reject the mechanism through setting up incubation data acquisition mechanism and useless card strip, reunion transport module, with three part independent operation, mutually noninterfere, carry out the cooperation work according to the process needs, realize independent hatching the subassembly setting, thereby increased and hatched the position in a storehouse, instrument efficiency of software testing is higher. The addition and removal of the whole reagent card strip are automatically completed, so that the automatic acquisition of reagent luminescence data and the automatic elimination of waste card strips are realized; through hatching the baffle, realize the automatic fixation and the location of card strip, operation process is simple and convenient, has improved work efficiency. And the heating and temperature control are realized, and the incubation result is more accurate.

Furthermore, the reagent card box module comprises a card box device, a scanning device is arranged on the card box device, and the scanning device can move relative to the card box device; the card box device is externally provided with a card hooking mechanism which can move relative to the card box device and can be inserted into the card box device. The reagent card box module of this scheme design, it can put into multiple different project reagent card box and a reagent card box simultaneously and can load a plurality of individual person's reagent card strips, disposes reagent card box simultaneously and targets in place to detect and also can detect reagent card box real-time status, but the scanner automatic reading reagent card box information moreover. And the automatic hooking and taking of the reagent card strip can be realized by the aid of a hooking and clamping device. To increase the number of reagent card strips that are placed in a single pass. The efficiency of supporting instrument is promoted.

Furthermore, the conveying mechanism comprises a first conveying plate, the first conveying plate is connected with the driving mechanism eight and can horizontally move under the action of the driving mechanism eight, a seventeenth driving mechanism and a second conveying plate are arranged on the first conveying plate, the seventeenth driving mechanism is connected with the second conveying plate, and the second conveying plate horizontally moves relative to the first conveying plate under the action of the seventeenth driving mechanism; the conveying plate II is provided with a conveying shifting fork and a driving mechanism nine, the driving mechanism nine is installed on the conveying plate II and connected with the driving mechanism nine, and the conveying shifting fork can vertically move under the action of the driving mechanism nine and is connected with the hook clamping mechanism.

In conclusion, compared with the prior art, the invention has the following beneficial effects:

(1) the invention can realize automatic sample entering and exiting and sample information reading;

(2) the invention can realize automatic conveying, cap pulling and cap closing of the sample tube;

(3) the invention can realize automatic shaking of the sample;

(4) the invention can realize automatic adding, removing and state detection of the suction head;

(5) the invention can realize simultaneous multi-sample test;

(6) the invention can realize automatic dilution and uniform mixing of samples;

(7) the invention can realize the automatic picking and transferring of the reagent card strip;

(8) the invention can realize movement and dynamic lighting;

(9) the invention can realize the detection of the liquid level of the sample adding;

(10) the invention adopts a modular design, and the whole machine is more quickly and flexibly integrated.

Drawings

The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:

fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a schematic structural diagram of a sample block.

FIG. 3 is a schematic structural diagram of the shake-up module.

Fig. 4 is a schematic structural view of a suction head module.

Fig. 5 is a schematic structural diagram of the cap pulling module.

FIG. 6 is a schematic structural diagram of a sample application module.

FIG. 7 is a schematic structural view of an incubation module.

Fig. 8 is a schematic structural diagram of a control module.

Fig. 9 is a schematic structural view of a transport module.

FIG. 10 is a schematic view of the construction of a reagent cartridge module.

FIG. 11 is a schematic view of the reagent cartridge module in another orientation.

The names corresponding to the reference numbers in the drawings are as follows:

1-a sample module, 2-a shaking module, 3-a sucker module, 4-a cap pulling module, 5-a sample adding module, 6-a control module, 7-an incubation module, 8-a transportation module, 9-a reagent box module, 10-a driving mechanism I, 11-a sensor I, 12-a synchronous belt pulley and a synchronous belt I, 13-a scanner, 14-an emergency position, 15-a driving mechanism II, 16-a driving mechanism I, 17-a sample frame limiting plate, 18-a mounting plate I, 19-a sample feeding shifting fork, 20-a synchronous belt pulley and a synchronous belt II, 21-an optical coupler I, 22-a stand column I, 23-a guide rail III, 24-a synchronous belt pulley and a synchronous belt III, 25-a driving mechanism IV, 26-a shaking mounting plate, 27-a shaking main body, 28-shaking upright post, 29-roller, 30-clamping mounting plate, 31-driving mechanism five, 32-limiting block, 33-sucker box mounting plate, 34-linear guide rail eight, 35-rack, 36-driving mechanism six, 37-limiting plate, 38-driving mechanism seven, 39-linear guide rail nine, 40-quadrangular knife mounting plate, 41-quadrangular knife, 42-upright post two, 43-sucker box placing plate, 44-sucker box set, 45-clamping jaw, 46-moving bracket, 47-clamping bracket, 48-driving mechanism eleven, 49-driving mechanism ten, 50-transverse plate connecting frame, 51-linear guide rail seven, 52-synchronous pulley and synchronous belt four, 53-driving mechanism twelve and 54-sensor two, 55-sample adding transverse plate I, 56-gun arranging mounting plate, 57-driving mechanism twelve, 58-guide rail ten, 59-gun arranging, 60-synchronous pulley and synchronous belt five, 61-guide rail eleven, 62-driving mechanism thirteen, 63-cable protection drag chain I, 64-sample adding upright post, 65-waste card baffle, 66-suction head removing bracket, 67-synchronous pulley and synchronous belt six, 68-driving mechanism sixteen, 69-driving mechanism fifteen, 70-cable protection drag chain II, 71-guide rail twelve, 72-incubation code ruler, 73-synchronous pulley and synchronous belt seven, 74-optical coupler two, 75-driving mechanism fourteen, 76-incubation upright post, 77-incubation bottom plate, 78-optical module, 79-card pushing bottom plate and 80-card pushing shifting fork, 81-a second main control board, 82-a third mounting board, 83-a first driving board, 84-a second driving board, 85-a third driving board, 86-a switching power supply, 87-a synchronous pulley and a synchronous belt eight, 88-a third cable protection drag chain, 89-a third sensor, 90-a seventeenth driving mechanism, 91-an eighth driving mechanism, 92-a third optical coupler, 93-a fourteen guide rail, 94-a nineteen driving motor, 95-a transport shifting fork, 96-code teeth, 97-a fifteen guide rail, 98-a reagent card box upright column, 99-a reagent card box bottom plate, 100-a reagent card box, 101-a nineteen driving mechanism, 102-a seventeen guide rail, 103-a hook card tooth, 104-a fourth cable protection drag chain, 105-a twenty-one driving mechanism and 106-a twenty driving mechanism, 107-hook clamp bottom plate, 108-synchronous pulley and synchronous belt nine, 109-back plate, 110-synchronous pulley and synchronous belt ten, 111-driving mechanism eighteen, 112-detection plate, 113-cover plate, 114-side plate, 115-scanning device, 116-guide rail sixteen, 117-hook clamp transverse plate, 118-synchronous pulley and synchronous belt eleven, 119-hook clamp shifting fork and 120-sample tube.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.

Example 1:

as shown in fig. 1, the present embodiment includes a frame, a sample module 1, a shaking module 2, a suction head module 3, a cap pulling module 4, a sample adding module 5, a control module 6, an incubation module 7, a transportation module 8 and a reagent card box module 9 are disposed on the frame, the transportation module 8 is connected to the reagent card box module 9 and the incubation module 7 at the same time, the sample adding module 5 is located above the moving track of the transportation module 8 and the sample adding module 5 is connected to the suction head module 3, the cap pulling module 4 is connected to the shaking module 2, the sample module 1 is connected to the cap pulling module 4 and the shaking module 2, the sample module 1, the shaking module 2, the suction head module 3, the cap pulling module 4, the sample adding module 5, the incubation module 7, the transportation module 8 and the reagent card box module 9 are connected to the control module 6, and after being arranged and connected as required, the sample module 1, the shaking module 2, the suction head module 3, the cap pulling module 4, the sample adding module 5, the incubation module 7, the transportation module 8 and the reagent card box module 9 are connected to meet the use requirements, the method adopts the biomedical technology and the chromatographic technology to analyze and process information of the chromatograph, the spectrum and the biosensor, is applied to immunity and rapid detection, realizes multi-channel and automatic detection, improves the field applicability of the detection, and presents the advantages of automation and simplification.

Example 2:

as shown in fig. 2, the sample module 1 of this embodiment includes a sample rack bottom plate, a first mounting plate 18 is disposed below the sample rack bottom plate, the first mounting plate 18 and the sample rack bottom plate are supported by a first column 22 to form a mounting space with an adequate bottom, the sample rack bottom plate is fixed to the rack, a sample rack is disposed on the sample rack bottom plate, the sample rack is located below the cap pulling module and the shaking homogenizing module and movably connected to the cap pulling module and the shaking homogenizing module, the sample rack is named as a sample rack to be tested, a sample rack to be tested and a sample rack to be tested according to different processes of the sample rack, a first driving mechanism 16, a synchronous belt wheel and a second synchronous belt 20 are disposed below the sample rack bottom plate, the synchronous belt is connected to a sample introduction fork 19, the sample introduction fork 19 is located in a corresponding slide way formed on the sample rack bottom plate, and the sample introduction fork 19 can move along the slide way. The sample introduction shifting fork 19 is connected with a first guide rail, and the sample introduction shifting fork 19 can move along the first guide rail. And the sample feeding shifting fork 19 is provided with a first optical coupler 21 at the limit position for limiting the movement distance. The first driving mechanism 16 drives the synchronous belt to move, so that the sampling shifting fork 19 moves in the slide way, and the sample frame to be tested is pushed to move horizontally on the bottom plate of the sample frame.

Be provided with horizontal bottom plate in sample frame bottom plate outside, install actuating mechanism two 15 on the horizontal bottom plate, synchronous pulley and hold-in range one 12 and scanner 13, be connected with horizontal shift fork on the hold-in range, when the sample frame that awaits measuring moves extreme position under actuating mechanism 16 effect, with the horizontal shift fork on the hold-in range that actuating mechanism two 15 are connected just with survey sample frame lock, make it become test sample frame, install guide rail two on the horizontal bottom plate, sample frame is connected the back with actuating mechanism two 15 and is connected with guide rail two simultaneously, and can remove along guide rail two. The second driving mechanism 15 drives the test sample rack to horizontally move relative to the sample rack bottom plate and read the bar code information through the scanner station.

A driving mechanism I10, a synchronous belt wheel and a synchronous belt III 24 are arranged below the sample rack bottom plate, the driving mechanism I10 is connected with a sample outlet shifting fork, the sample outlet shifting fork is located in a corresponding slide way formed in the sample rack bottom plate, and the sample outlet shifting fork can move along the slide way. The sample outlet shifting fork is connected with a third guide rail 23 and can move along the third guide rail 23. After the scanning is completed, the sample outlet shifting fork moves to a preset position, the sample outlet shifting fork hooks the test sample rack, the sample rack can horizontally move relative to the sample rack bottom plate under the action of the driving mechanism I10, meanwhile, the connection relation between the sample rack and the synchronous belt in the driving mechanism II 15 is removed, the sample rack becomes a tested sample rack along with the movement of the test sample rack, a sample rack limiting plate 17 is arranged on the sample rack bottom plate and used as the limit position and the guide of the movement, and a sensor I11 is further arranged on the sample rack bottom plate and used as the movement position for detection. An emergency treatment position 14 is reserved on a sample frame bottom plate, and the application scene of a complete machine is further improved.

By setting the moving direction of the sample rack under the action of the driving mechanism III 16 and the moving direction of the sample rack under the action of the driving mechanism I10 to be parallel, the moving direction of the sample rack under the action of the driving mechanism II 15 and the moving direction of the sample rack under the action of the driving mechanism III 16 are set to be vertical. Finally, circulation of the sample rack is realized.

The working principle is as follows:

a sample to be detected is placed in a sample frame to be detected on the right side of a bottom plate of the sample frame, when the sample frame is placed in, attention needs to be paid to the fact that a bottom gap needs to be aligned with a limiting block plate 17 of the sample frame (foolproof function), a driving mechanism III 16, a synchronous belt wheel, a synchronous belt II 20 and a sample introduction shifting fork act together to move the sample frame to a preset position, and a sensor I11 is arranged to detect the state of the sample frame; the second driving mechanism 15, the synchronous belt wheel and the first synchronous belt 12 act together to move the sample rack to a preset position, and the scanner 13 reads bar code information; after a sample is processed, the first driving mechanism 10, a synchronous belt wheel, a third synchronous belt 24 and a sample outlet shifting fork act together to move a sample rack to a preset position, and the state of the sample rack is detected through a first sensor 11; and an emergency treatment position 14 is arranged in the sample device to increase the application environment of the matched instrument. The scheme can realize automatic movement of the sample rack; the sample information can be automatically read; the in-place detection of the sample rack can be realized; the emergency treatment sample can be independently placed; a plurality of groups of sample racks can be placed at the same time; the positioning direction limit of the sample rack can be realized.

Example 3:

as shown in fig. 3, the shaking module 2 includes a supporting mechanism, the supporting mechanism is mainly composed of two shaking mounting plates 26 and two shaking upright columns 28, wherein the two shaking upright columns 28 are vertically arranged, and the shaking mounting plates 26 are fixed with the two shaking upright columns 28 at the same time. The supporting mechanism is provided with a shaking main body 27 and a driving mechanism IV 25, and the driving mechanism IV 25 adopts a stepping motor as the shaking driving mechanism. Be provided with the through-hole that is used for installing the sample test tube on shaking even main part 27, shaking even apron in shaking even main part 25 top surface installation, shake even apron and expose the through-hole, shake even apron and be provided with the sample frame adapter, every sample frame adapter sets up and goes out at the through-hole mouth, it is fixed to the sample test tube in the through-hole through shaking even apron and sample frame adapter, it need not press from both sides tightly the test tube to shake even process, it can to carry out preliminary adaptation through sample frame adapter, the quantity of through-hole is decided according to concrete sample test tube quantity, this embodiment preferably adopts 5 through-holes. This scheme is shaken even porous design of main part 27, can pack into different project reagents of many people respectively and carry out the mixing, improves work efficiency.

The driving mechanism four 25 is fixed by a shaking motor support and a shaking mounting plate 26 or a shaking upright post 28, a deep groove ball bearing is arranged in the shaking motor support, a shaking motor shaft sleeve is arranged between the shaking motor support and the shaking main body 27, the shaking motor shaft sleeve is connected with the shaking main body 27, the driving mechanism four is arranged on the shaking motor support, the output end of the driving mechanism four penetrates through the deep groove ball bearing and is connected with the shaking motor shaft sleeve, the rotation of the driving mechanism four 25 is transmitted to the shaking main body 27 in time, the other end of the shaking main body 27 is connected with the shaking main body 27 through an oil-free bushing and a rotating shaft, the rotation of the shaking main body 27 is met, and the support is also realized. Through this kind of structural design, actuating mechanism four 25 rotates, can drive shake even main part 27 and rotate around its self axis, according to actual turned angle needs, the biggest turned angle be 90 can, sample test tube level has been realized shaking even this moment. The side wall of the shaking mounting plate 26 is provided with a shaking resetting optocoupler, when the shaking main body 27 rotates to 90 degrees, the shaking main body 27 is just contacted with the shaking resetting optocoupler, and the driving mechanism receives a signal to reverse.

A clamping mechanism and a driving mechanism five 31 are arranged below the shaking main body 27, and a screw rod motor is preferably adopted as the driving mechanism five 31 as the clamping motor, so that the whole structure of the device is compact and the function is realized conveniently. Clamping mechanism is including shaking even holder, briquetting and linear guide five, and the briquetting is fixed bottom shaking even holder, and shakes even holder and linear guide five and connect, shakes even holder and can move along linear guide five, and linear guide five is fixed on shaking even main part 27, and the briquetting is provided with pressure spring with shaking between the even holder, and pressure spring is connected with the briquetting and shaking even holder simultaneously, and during operation sample test tube bottom is located the briquetting and shakes between the even holder. The clamping mounting plate 30 is provided with a roller 29, and the clamping mounting plate 30 moves linearly under the action of the driving mechanism five to enable the roller 29 to enter between the pressing block and the shaking uniformly clamping seat and then to contact with the shaking uniformly clamping seat. The gyro wheel 29 adopts the multiunit, in order to facilitate the gyro wheel 29 can enter into the briquetting and shake even between the holder, so will shake even holder and the face design of gyro wheel 29 contact for the inclined plane, the briquetting forms an opening with the face that shakes even holder and is close to relatively, and the gyro wheel of being convenient for can squeeze into more easily like this, loosens the function and can realize fast. The multiple points enter the stress simultaneously, so that the actions can be realized simultaneously, and the loosening of all the sample test tubes can be synchronously performed.

The driving mechanism five 31 and the clamping mounting plate 30 are connected by a screw-nut pair, and the rotation of the driving mechanism five is changed into the linear movement of the clamping mounting plate 30. Be provided with limit guide on shaking even mounting panel 26, be fixed with the sliding sleeve on pressing from both sides tight mounting panel 30, the sliding sleeve cover is on limit guide, presss from both sides tight mounting panel 30 and can remove along limit guide to the limited movement orbit.

And a clamping reset optical coupler is further arranged on the mounting seat of the driving mechanism five 31 and is positioned below the clamping mounting plate 30. The moving distance of the movable clamping mounting plate 30 is limited by clamping the reset optical coupler, and when the movable clamping mounting plate moves to the lowest position, the movable clamping mounting plate is in contact with the clamping reset optical coupler, and the driving mechanism five 31 stops rotating or rotates reversely. The clamping and mounting plate 30 is further provided with a limiting block 32 for limiting the position.

Fix 5 sample test tubes in shaking even main part 27 through sample frame adapter and shake even apron simultaneously, shake even process then and be: through the assembly shake even step motor on shaking even motor support through deep groove ball bearing, oilless bush, rotatory pivot and shake even motor shaft sleeve's connection with rotary power conveying shake even main part 27, will shake even main part 27 and rotate 90 degrees rotary motion that realize the sample test tube promptly to the realization is to the stirring mixing of sample test tube reagent. The clamping and unclamping process is then: the clamping screw motor drives the clamping mounting plate 30 to move linearly upwards, so that the roller 29 enters between the shaking clamp holder and the pressing block, the pressing block is fixed, the shaking clamp holder moves along the linear guide rail after being extruded, the gap between the shaking clamp holder and the pressing block is reduced, and the sample test tube is clamped; the clamping screw motor rotates reversely to drive the clamping mounting plate 30 to move linearly downwards, the idler wheel 29 gradually withdraws from between the shaking-up clamp holder and the pressing block, the shaking-up clamp holder and the pressing block are gradually flicked open through the elasticity of the pressing spring, the shaking-up clamp holder moves along the linear guide rail V under the action of the elasticity of the pressing spring, and finally the sample test tube is loosened. The shaking and clamping loosening integrated device is compact in structure and reliable in operation, can be used for simultaneously carrying out simulated manual operation shaking detection on a plurality of groups of sample test tubes, and not only meets the detection requirement of multiple samples, but also can realize the requirements of high-efficiency operation and automation.

Example 4:

as shown in fig. 4, the suction head module 3 comprises a suction head box mounting plate 33, the suction head box mounting plate 33 is fixed with the frame through a second upright post 42, a suction head box placing plate 43 is arranged on the suction head box mounting plate 33, a suction head box sleeve 44 is integrally positioned on the suction head box placing plate 43, a driving mechanism six 36 is arranged on the suction head box mounting plate 33, the output end of the driving mechanism six 36 is sleeved with a gear, the gear is meshed with a rack 35, the rack 35 is connected with the suction head box placing plate 43, under the action of a driving mechanism six 36, the rack 35 moves to pull the suction head box set 44 on the suction head box placing plate 43 to move, the suction head box set 44 is connected with the sample adding module in the moving process, a linear guide rail eight 34 is further arranged on the suction head box mounting plate 33, the suction head box placing plate 43 is connected with the linear guide rail eight 34 and can move along the linear guide rail eight 34, and a limiting plate 37 is mounted at the end head of the linear guide rail eight 34; the suction head box mounting plate 33 is provided with a piercing mounting plate, the driving mechanism seven 38 is fixed on the piercing mounting plate, the driving mechanism seven 38 is connected with a quadrangular knife mounting plate 40, the quadrangular knife mounting plate 40 and the piercing mounting plate are connected through a linear guide rail nine 39, a quadrangular knife 41 is mounted on the quadrangular knife mounting plate 40 and serves as a piercing head, and the quadrangular knife 41 moves in the vertical direction under the action of the driving mechanism seven 38. The sucker box suit 44 is placed on the sucker box placing plate 43, and the movement of the sucker box can be realized through the gear and driving mechanism six 36, the rack 35 and the linear guide rail eight 34; the quadrangular knife 41 is arranged on the quadrangular knife mounting plate 40 and can be driven by the seven driving mechanisms 38 and the nine linear guide rails 39 to realize the motion in the vertical direction, so that the film breaking of the reagent card strip film is realized.

Example 5:

as shown in fig. 5, the cap pulling module 4 comprises a transverse plate connecting frame 50, the transverse plate connecting frame 50 is a plate, the transverse plate connecting frame 0 is fixed with the frame, a driving mechanism ten 49 is mounted on the transverse plate connecting frame 50 as a power mechanism of the moving mechanism, the driving mechanism ten 49 is connected with the moving bracket 46, the driving mechanism ten 49 preferably adopts a screw motor, the driving mechanism ten 49 is connected with the moving bracket 46 through a screw nut pair structure, and the driving mechanism ten 49 rotates to drive the moving bracket 46 to move linearly. The movable support 46 is also arranged on the transverse plate connecting frame and movably connected with the transverse plate connecting frame, the movable support 46 is of an L-shaped structure, a linear guide rail six is arranged on the transverse plate connecting frame 6 and is fixed with the transverse plate connecting frame, and the axial direction of the linear guide rail six is the same as the moving direction of the movable support 46. The moving bracket 46 is connected to the linear guide six through a slide groove and is capable of moving in the direction of the linear guide six axis.

The movable support 46 is provided with a clamping mechanism, the clamping mechanism comprises a clamping support 47 and an eleventh driving mechanism 48 which are connected with each other, the eleventh driving mechanism 48 also adopts a screw motor, the screw motor is connected with the clamping support 47 through a screw nut pair structure, and the eleventh driving mechanism 48 drives the clamping support 47 to move linearly. The clamping bracket 47 is mounted on the moving bracket 46 and movably connected with the moving bracket 46, a linear guide rail seven 51 is arranged on the moving bracket 46, the linear guide rail seven 51 is fixed with the moving bracket 46, and the moving directions of the linear guide rail seven 51 and the clamping bracket 47 are the same. The clamp bracket 47 is connected to the linear guide seven 51 via a slide groove and is movable in the axial direction of the linear guide seven 51.

The clamping support 47 is provided with a cap pulling rotating shaft, the clamping support 47 is provided with a first deep groove ball bearing, and the top end of the cap pulling rotating shaft penetrates through the first deep groove ball bearing and is connected with the first deep groove ball bearing, so that the cap pulling rotating shaft and the clamping support 47 are fixed.

The rotating mechanism comprises a driving mechanism twelve 53 and a transmission mechanism, the transmission mechanism is composed of a synchronous belt wheel and a synchronous belt four 52, the driving mechanism twelve 53 adopts a stepping motor, the driving mechanism twelve 53, the synchronous belt wheel and the synchronous belt four 52 are all installed on the movable support 46, the output end of the driving mechanism twelve 53 is connected with a driving wheel in the transmission mechanism, a driven wheel in the transmission mechanism is connected with a clamping jaw support, the clamping jaw support is positioned below the movable support 46, a plurality of clamping jaws 45 are installed on the clamping jaw support, and a cap pulling rotating shaft penetrates through the driven wheel in the transmission mechanism and then is connected with the corresponding clamping jaw 45, so that the rotating and clamping actions are not influenced, and the rotating and clamping actions are not interfered with each other. Clamping jaw pads are installed on the clamping jaws 45, a sensor II 54 is arranged at the clamping jaw to detect whether a cap of the sample test tube is in a position, 5 groups of clamping jaws 45 are preferably selected in the scheme, meanwhile, the cap pulling or cap covering action of 5 groups of sample test tubes is achieved, the clamping jaws 45 are of an opening structure, the end portions of the sample test tubes are located in openings, the opening size change is achieved by pulling the cap rotating shafts to move, and the clamping jaws 45 are driven to clamp or loosen when the cap pulling rotating shafts move along with the clamping supports 47. The driven wheel is connected with the movable support 46 through a second deep groove ball bearing, and rotation control over the clamping jaw support is achieved.

For the convenience of control, a driving plate is further provided, and the driving plate is mounted on the top of the moving bracket 46 through a transverse plate, and the transverse plate is fixed to the top of the moving bracket 46. The driving plate is connected with a drag chain, and is simultaneously connected with a driving mechanism ten 49, a driving mechanism eleven 48 and a driving mechanism twelve 53. The driving mechanism 49 and the transverse plate form a screw-nut pair connection, so that the structure is more reasonable.

The prior art is low in efficiency and complicated in manual operation, most of in vitro diagnosis analyzers still adopt manual work to finish cap pulling extraction and cap storage of sample test tubes, and the manual work load is large. Or only the cap-pulling extraction function is adopted, and the reagent sample cannot be extracted and detected for multiple times. The requirements for equipment compatibility, efficient operation and automation at present cannot be met. And the intelligent degree of equipment is not high, current external diagnostic analyzer has the general lack of detection to the cap of sample test tube, if there is the test tube sample that does not pull out the cap very easily to lead to equipment to appear unusually, this scheme is that pull out the cap of sample test tube and pass through integration, can pull out the cap to handle to multiunit sample test tube simultaneously, compact structure, the operation is reliable high-efficient, can detect the existence of its cap to multiunit sample test tube simultaneously, realize closed-loop control on the procedure, avoid equipment to appear unusually, realize the high-efficient operation of equipment and automatic demand, compact structure, structurally after the combination, shorten middle latency, improve work efficiency.

The working principle of the scheme is as follows:

(1) and (3) pulling the cap: the driving mechanism 49 drives the 5 clamping jaws 45 to move to the cap pulling position of the sample tube 120 in the Z direction at the same time, then the driving mechanism eleven 48 acts to drive the clamping support 47 and the cap pulling rotating shaft to move linearly upwards together, so that the 5 clamping jaws 45 perform tightening action together, the 5 sample tube 120 caps are clamped, then the driving mechanism twelve 53 works to drive the synchronous belt and the clamping jaw support to rotate the 5 clamping jaws 45, and meanwhile, the driving mechanism 49 works to drive the clamping jaws 45 to move upwards, so that the cap pulling action of the 5 sample tubes 120 is realized;

(2) capping process: after the cap is pulled out, the driving mechanism 49 works to drive the 5 clamping jaws 45 to move downwards, meanwhile, the driving mechanism twelve 53 works to drive the synchronous belt and the clamping jaw support to enable the 5 clamping jaws 45 to rotate until the clamping jaws 45 move to the cap covering position, and when the test tube cap enters the sample tube 120, the driving mechanism eleven 48 synchronously acts to drive the clamping support 47 and the cap pulling rotating shaft to linearly move downwards together, so that the 5 clamping jaws 45 are opened, and the cap covering of the 5 sample tube 120 cap is realized.

Example 6:

as shown in fig. 6, the sample adding module includes a sample adding column 64, the sample adding column 64 is fixed to the frame, and the sample adding column 64 is a square structure. Be provided with application of sample diaphragm one 55 between application of sample stand 64, and application of sample diaphragm one 55 is fixed respectively on corresponding application of sample stand 64, installs actuating mechanism thirteen 62 on application of sample diaphragm one 55, is provided with application of sample diaphragm two between application of sample diaphragm one 55 as row rifle mounting panel 56, arranges rifle mounting panel 56 and installs on application of sample diaphragm one 55 and through synchronous pulley and hold-in range five 60 and be connected with actuating mechanism thirteen 62 to remove along application of sample diaphragm one 55 under actuating mechanism thirteen 62 effects. The first cable protection drag chain 63 is connected to the thirteen drive mechanism 62, the guide rail ten 58 is mounted on the sample loading cross plate one 55, and the gun arraying mounting plate 56 is connected to the guide rail ten 58 and can move along the guide rail ten 58. Install synchronous pulley and hold-in range five 60 on application of sample diaphragm 55, its action wheel and actuating mechanism thirteen 62 are connected, synchronous pulley's action wheel and from installing the hold-in range on the driving wheel, it has a plurality ofly from the driving wheel, can realize connecting the hold-in range like this, and hold-in range partly is located application of sample diaphragm 55, arrange the rifle mounting panel 56 and hold-in range and be located the part on application of sample diaphragm 55 and be connected, actuating mechanism thirteen 62 makes row rifle mounting panel 56 move on application of sample diaphragm 55 along guide rail ten 58 through driving the hold-in range removal. A line gun 59 is provided on the line gun mounting plate 56, while the line gun 59 is capable of vertical movement and insertion into a pipette box or a shake-up body or a sample rack.

The arrangement gun mounting plate 56 is provided with a drive mechanism twelve 57, the arrangement gun mounting plate 56 is provided with a synchronous pulley and a synchronous belt, a driving wheel of the synchronous pulley is connected with the drive mechanism twelve 57, the synchronous belt is simultaneously connected with a driving wheel and a driven wheel in the synchronous pulley, and the sample adding mechanism is mounted on the arrangement gun mounting plate 56, is connected with the synchronous belt and moves along the arrangement gun mounting plate 56 under the action of the drive mechanism twelve 57. The gun-discharging mounting plate 56 is mounted with a guide rail eleventh 61, and the gun-discharging 59 is connected to the guide rail eleventh 61 and is movable along the guide rail eleventh 61. The drive mechanism thirteen 62 and the drive mechanism twelve 57 are both stepping motors.

The scheme provides a device capable of realizing the functions of automatic adding and removing of suction heads, sample adding and sample discharging and the like, and the plane horizontal motion of the whole sample adding device is realized by the transmission of a stepping motor and a synchronous belt, namely, a discharging gun 59 is moved to each preset position in the structure (for example, a suction head adding position, a suction head removing position, a sample adding position, a sample discharging position and the like). Realize the vertical direction motion through step motor, realize that the suction head adds and removes promptly, also can realize stretching into the suction head simultaneously and carry out the application of sample in the sample tube and stretch into the suction head and carry out closely the stock layout with the stock layout mouth, have sample liquid level height simultaneously concurrently and detect the function, accurate and the efficient device of the automatic completion of full flow. The scheme is matched with the integrated reagent card and the disposable suction head, and the disposable suction head can be automatically added and installed; automatically removing the used waste suction head; automatic sample adding and automatic sample discharging; high-precision sample adding is realized after the liquid level height of the sample is automatically identified; automatically monitoring the state of the suction head in real time; and at most five groups of gun heads can move independently to perform stock layout.

Each stepping motor is matched with one group of linear guide rails and matched with mutually independent motor driving plates to realize the mutually independent movement of the five groups of gun heads. And the drive plate is provided with an encoder which is linked with a corresponding photoelectric switch to realize closed-loop control so as to improve the motion precision of the whole device. Each group of gun heads is provided with a liquid level detection plate, and the liquid level detection plates are matched with the conductive suction heads for use, so that accurate sample suction of different sample amounts can be realized, the sample suction precision of the whole device is improved, and the overall performance of a matched instrument is further improved. And the state of the suction head is monitored in real time, so that the matched instrument is more intelligent.

The synchronous belt and the pressing block are connected in series to realize the transmission of a power source, so that the XY plane horizontal motion of the arraying gun 59 and the arraying gun mounting plate 56 is realized, and the vertical motion is realized by the screw rod stepping motor in the arraying gun 59 and the arraying gun mounting plate 56. Namely, the sample adding device is moved to each preset position in the mechanism.

The scheme can realize automatic adding and removing of the suction head; automatic sample adding and sample discharging can be realized; the conductive suction head is matched with a liquid level detection system, and the depth of the suction head inserted below the liquid level of a sample is accurately controlled, so that the sample adding precision requirement of the whole device is guaranteed; the five groups of gun heads can move independently, so that the independent movement of at most five groups of gun heads can be realized simultaneously, and the efficiency of the whole device can be improved; the state of the suction head can be monitored in real time, when the suction head is in an abnormal state (such as unsuccessfully adding, successfully removing, falling in the midway of operation and the like), the suction head can be fed back in time, and corresponding correction can be carried out if necessary, so that the whole device is more intelligent; the gun head can move along XYZ axes in the internal space of the mechanism, thereby greatly improving the flexibility of the instrument and the working efficiency.

Example 7:

as shown in fig. 7, the incubation module includes incubation data acquisition mechanism and waste card strip removing mechanism, the incubation data acquisition mechanism includes incubation bottom plate 77, a plurality of incubation columns 76 are installed at the bottom of the incubation bottom plate 77 for supporting, a channel for installing reagent card strips is arranged on the incubation bottom plate 77, the reagent card strips are installed in the corresponding channel, in this embodiment, 30 reagent card strips can be incubated at most once, and the test efficiency is improved.

Be provided with actuating mechanism fourteen 75, synchronous pulley and hold-in range seven 73 on hatching bottom plate 77, its action wheel and follow driving wheel all install on hatching bottom plate 77 and can rotate round self axis, the hold-in range is connected with action wheel and follow driving wheel simultaneously, wherein action wheel and actuating mechanism fourteen 75 are connected, data collection station and hold-in range are connected, and data collection station can carry out horizontal migration through the effect of hold-in range under actuating mechanism fourteen 75 drives.

A guide rail twelve 71 is mounted on the incubation bottom plate 77, and the data collector is connected to the guide rail twelve 71 and can move along the guide rail twelve 71. Therefore, the data acquisition unit realizes movement and limiting through the synchronous belt and the guide rail twelve 71, and the whole movement track is limited, so that the working process is controllable. Incubation base plate 77 is last still to be provided with incubation yardstick 72, is provided with hot plate and temperature sensor on incubation base plate 77, and temperature sensor is connected with the hot plate. The heating plate is preferably a heated aluminum substrate. And a second optocoupler 74 is also arranged on the incubation bottom plate 77.

The data collector is further connected with a second cable protection tow chain 70 for protecting the cable in the moving process, and comprises an optical module 78, wherein the optical module 78 is arranged on the guide rail twelve 71 and can move along the guide rail twelve 71. The optical module 78 is provided with a driving mechanism fifteen 69, the output end of the driving mechanism fifteen 69 is connected with a PMT photomultiplier, and the driving mechanism fifteen 69 can drive the PMT photomultiplier to horizontally move to acquire data of the reagent card strip.

The waste card strip removing mechanism and the incubation data acquisition mechanism are located on the same straight line, and the transport module moves along the same direction to achieve the matched operation of the waste card strip removing mechanism and the incubation data acquisition mechanism. Waste card strip rejection mechanism is including pushing away card bottom plate 79, still install actuating mechanism sixteen 68 on pushing away card bottom plate 79, synchronous pulley and hold-in range six 67, synchronous pulley's action wheel and follow driving wheel all install push away card bottom plate 79 on and can rotate round self axis, the hold-in range is connected with the action wheel with follow driving wheel simultaneously, action wheel and actuating mechanism sixteen 68 are connected, connect through hold-in range and push away card shift fork 80, utilize actuating mechanism sixteen 68's effect to drive the hold-in range and remove, thereby make and push away card shift fork 80 and carry out horizontal migration. A guide thirteen is mounted on the card-pushing bottom plate 79, and a card-pushing fork 80 is connected to the guide thirteen and is movable along the guide thirteen. Through the combined action of the guide rail thirteen and the synchronous belt, the movement and the limiting are realized, and the whole movement track is limited, so that the working process is controllable. A waste card guard 65 and tip ejection bracket 66 are also provided on the support base 8. The waste card strip removing mechanism is mainly used for removing the tested waste card strips.

After the transport module transports the reagent card strip to the incubation module, the reagent card firstly carries out reagent reaction, and after the reaction is finished, the lighting module 78 is under the combined action of the fifteen 69 driving mechanisms and the fourteen 75 driving mechanisms. The reagent card can move transversely and longitudinally, and lighting can be finally realized for reagent cards at different incubation positions; the transportation module transports the reagent card strip which finishes lighting to a preset position of a card pushing bottom plate 79, the reagent card is pushed into the waste card baffle 65 under the combined action of a sixteen driving mechanism and a card pushing shifting fork 80, and the reagent card falls along the inclined plane; the exhaust gun moves to a preset position for withdrawing the suction head, the waste suction head is withdrawn in the suction head withdrawing bracket 66, and the waste suction head falls along the inclined plane; and the incubation code scale 72 is matched to realize closed-loop control so as to improve the motion precision of the whole module.

The scheme provides a reagent card strip automatic heating incubation and waste reagent card strip automatic elimination device which can realize the functions of reagent card strip automatic heating incubation, waste reagent card strip automatic elimination and the like, and realize the automatic collection of reagent luminescence data and the automatic elimination of waste card strips through the transmission of a stepping motor and a synchronous belt; the automatic fixing and positioning of the clamping strips are realized through the incubation partition plate; real-time feedback and control of incubation temperature are realized by heating the PCB and the temperature sensor. This scheme is through step motor and linear guide collocation, and the cooperation is with mutually independent motor drive plate, realizes useless card push pedal and PMT's independent motion, makes PMT can remove the card strip top that needs data collection wantonly, and useless card rejects and data acquisition mutually independent, and mutual noninterference has improved the efficiency of test.

The waste card removing mechanism and the incubation mechanism are relatively independent, the transfer of the card strips is completed through the transportation assembly, the waste card removing and the heating incubation can work simultaneously, and the testing efficiency is improved. Namely, the transfer and reaction test of the card strip between the rejecting mechanism and the incubating mechanism is realized through the transporting mechanism.

According to the scheme, 30 reagent clamping strips can be incubated at most once, so that the test efficiency is improved; the incubation temperature can be fed back and controlled in real time; PMT can read the luminous data of the reagent card strip of any number bin randomly; the incubation bin and the removing mechanism work separately and independently without mutual interference.

Example 8:

as shown in fig. 8, the control template 6 is composed of a second main control board 81, a third mounting board 82, a driving board 83, a second driving board 84, a third driving board 85 and a switch circuit 86, and the working process is precisely controlled by these structures, which are conventional structures and can be directly purchased.

Example 9:

as shown in fig. 9, the transporting mechanism includes a first transporting plate, the first transporting plate is connected with an eighth driving mechanism 91 and can horizontally move under the action of the eighth driving mechanism 91, a seventeen driving mechanism 90 and a second transporting plate are arranged on the first transporting plate, the seventeen driving mechanism 90 is connected with the second transporting plate, and the second transporting plate horizontally moves relative to the first transporting plate under the action of the seventeen driving mechanism 90; the conveying plate II is provided with a conveying shifting fork 95 and a driving mechanism nine, the driving mechanism nine is installed on the conveying plate II and connected with the driving mechanism nine, and the conveying shifting fork can vertically move under the action of the driving mechanism nine and is connected with the hook clamping mechanism. The transport shifting fork 95 can move in the vertical direction under the drive of the drive mechanism nine 94, can move longitudinally along the linear guide rail fourteen 93 under the drive of the drive mechanism seventeen 90, and can move transversely along the linear guide rail fifteen 97 under the drive of the drive mechanism eight 91 driving the synchronous pulley and the synchronous belt eight 87. The reagent cards can finally be transported to and removed from the respective predetermined positions of the incubation module. The state of the reagent card can be monitored by being assisted with the sensor III 89, and closed-loop control can be realized by being matched with the code teeth 96 and the optical coupler III 92 so as to improve the motion precision of the whole module. Cable protection tow chain three 88 is used for cable protection.

Example 10:

as shown in fig. 10 and 11, the reagent cartridge module comprises a cartridge holder having a hollow interior box structure consisting of a reagent cartridge bottom plate 99 at the bottom, side plates 114 at the sides, a rear plate 109 and a cover plate 113 at the top, the reagent cartridge bottom plate 99 being supported by reagent cartridge posts 98, one side wall of the cartridge holder being provided with an opening through which the reagent cartridge 100 is inserted and removed. Be provided with actuating mechanism eighteen 111 on the lateral wall of cassette ware, actuating mechanism eighteen 111 passes through synchronous pulley and hold-in range ten 110 and connects, and scanning device 115 and hold-in range are connected, move through actuating mechanism eighteen 111 and drive the hold-in range and remove to make scanning device 115 remove, read different position reagent card box information. In contrast, the side wall of the cartridge housing in which the scanner 115 is mounted is provided with a plurality of apertures, one for each reagent cartridge 100, for easy reading. And a detection plate 112 is also provided at the side wall of the cartridge.

In order to define the moving track of the scanning device 115, a guide rail sixteen 116 is provided on the sidewall of the cartridge, and the scanning device 18 is connected with the guide rail sixteen 116 and can move along the guide rail sixteen 116.

And the hook card mechanism includes hook card bottom plate 107 and hook card ware, the hook card ware is installed on hook card bottom plate 107, hook card bottom plate 107 is located the cassette ware below, install actuating mechanism nineteen 101 and synchronous pulley and hold-in range nine 108 on the hook card bottom plate 107, actuating mechanism nineteen 101 and synchronous pulley and hold-in range nine 108 are connected, form transmission device, hook card ware and hold-in range are connected, the hook card ware passes through the hold-in range pulling can horizontal migration under actuating mechanism nineteen 101's effect like this, be provided with guide rail seventeen 102 on the hook card bottom plate 107, hook card ware and guide rail seventeen 102 are connected and can be removed along guide rail seventeen 102. Hook code teeth 103 are also provided on the hook base plate 107.

The hook clamp device comprises a hook clamp transverse plate 117 and a vertical hook clamp, the hook clamp transverse plate 117 is connected with a guide rail seventeen 102 and can move along the guide rail seventeen 102, the vertical hook clamp is installed on the hook clamp transverse plate 117, a driving mechanism twenty 106, a synchronous belt wheel and a synchronous belt eleven 118 are arranged on the hook clamp transverse plate 117, the driving mechanism twenty 106 is connected with the synchronous belt wheel and the synchronous belt eleven 118, a transmission mechanism is formed, the vertical hook clamp is connected with the synchronous belt, and the vertical hook clamp can horizontally move under the action of the driving mechanism twenty 106 through the synchronous belt. Be provided with guide rail eighteen on hook card diaphragm 117, vertical hook card and guide rail eighteen are connected and can remove along guide rail two. A cable protection drag chain four 104 is connected to the drive mechanism twenty 106. The eighteen 111, twenty 106 and nineteen 101 drive mechanisms are preferably stepper motors.

The vertical hook clamp comprises a mounting plate, a hook clamp shifting fork 119 and a driving mechanism twenty-one 105, and the mounting plate is connected with the guide rail eighteen and can move along the guide rail eighteen; the actuating mechanism twenty-one 105 is located on the mounting panel, the hooking and clamping fork 119 is connected with the actuating mechanism twenty-one 105, the hooking and clamping fork 119 can move in the vertical direction under the action of the actuating mechanism twenty-one 105, and the hooking and clamping fork 119 can be inserted into the card box device. The driving mechanism twenty-first 105 preferably adopts a screw rod motor, a screw rod of the screw rod motor and the end of the hook and clamp shifting fork 119 form a screw rod and nut pair structure, and the rotation of the driving mechanism twenty-first 105 is changed into the vertical movement of the hook and clamp shifting fork 119.

The principle of the scheme is as follows:

the reagent card box 100 is placed in each preset position, the detection plate 112 is arranged to monitor the state of the reagent card box 100 in real time, the hook-and-clamp shifting fork 119 is driven by the driving mechanism twenty-one 105 to move in the vertical direction, the driving mechanism twenty-106 is driven to move longitudinally, the driving mechanism nineteen 101 is driven to move transversely along the linear guide rail seventeen 102, finally, reagent cards can be hooked from the reagent card boxes 100 and placed into a transportation module, and the hook-and-clamp code teeth 103 are matched to realize closed-loop control so as to improve the movement precision of the whole module. The scanning device 115 can move transversely under the drive of the eighteen 111 driving mechanisms, so that the information of each test cartridge 100 can be read. The invention can realize the simultaneous placement of reagent cards of various different detection items; the state of the reagent card box can be automatically detected; the automatic reading of the information of the reagent card box can be realized; can realize the automatic hooking of the reagent card strip.

The principle of the invention is as follows:

placing reagent cartridges of different projects into a reagent cartridge module and reading information of the reagent cartridges → placing a suction head into a suction head cartridge module → placing samples to be tested into the sample module → inputting test information of the samples to be tested into test software and clicking 'start test' → transportation module to hook reagent card strips of corresponding projects → transporting to a puncturing position to break membranes → transporting to a loading position → adding a suction head into a loading module → a sample module reading sample information → pulling a cap module transporting a sample tube into a shaking module to shake up → pulling a test tube cap → a loading module sucking samples → discharging into the reagent card strips to dilute and mix → discharging the reagent card strips into a sample discharging opening → transporting module transporting to an incubation module to incubate for incubation → lighting module taking out the reagent card strips of which lighting is finished → transporting to a discarding position to discard cards → unloading a suction head module → pulling a cap module combining a test tube cap → transporting a sample tube to a test tube module to remove the samples. The invention can realize automatic sample entering and exiting and sample information reading; the sample tube can be automatically conveyed, pulled out of the cap and closed; the sample can be automatically shaken up; the automatic adding and removing of the suction head and the state detection can be realized; the simultaneous multi-sample test can be realized; the automatic dilution and uniform mixing of the sample can be realized; the automatic picking and transferring of the reagent card strip can be realized; the mobile and dynamic lighting can be realized; and the liquid level detection of the sample adding can be realized. And the modular design is adopted, so that the whole machine is integrated more quickly and flexibly.

The above embodiments are provided to further explain the objects, technical solutions and advantages of the present invention in detail, it should be understood that the above embodiments are merely exemplary embodiments of the present invention and are not intended to limit the scope of the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. Dry-type fluorescence immunoassay appearance based on realize multichannel and automated inspection, including the frame, its characterized in that: be provided with the sample module in the frame, shake even module, suction head module, pull out cap module, application of sample module, hatch module, transportation module and reagent card box module, and the transportation module is connected with reagent card box module and incubation module simultaneously, and the application of sample module is located transportation module and removes track top and application of sample module and suction head module connection, pulls out the cap module and shakes even module connection, and the sample module with pull out the cap module and shake even module connection.

2. The dry fluoroimmunoassay analyzer for realizing multi-channel and automatic detection according to claim 1, wherein: the sample module comprises a sample frame bottom plate, the sample frame bottom plate is fixed with the rack, a sample frame is arranged on the sample frame bottom plate, and the sample frame is positioned below the cap pulling module and the shaking module and is movably connected with the cap pulling module and the shaking module; a first driving mechanism is arranged below the bottom plate of the sample frame, the first driving mechanism is connected with a sample feeding shifting fork, the sample feeding shifting fork is connected with the sample frame, and the sample frame and the sample feeding shifting fork can horizontally move relative to the bottom plate of the sample frame under the action of the first driving mechanism; a transverse bottom plate is arranged outside the sample frame bottom plate, a second driving mechanism and a scanner are installed on the transverse bottom plate, the sample frame can be connected with the second driving mechanism after moving under the action of the first driving mechanism, and horizontally moves relative to the sample frame bottom plate under the action of the second driving mechanism and passes through a scanner station; and a third driving mechanism is arranged below the bottom plate of the sample frame, the third driving mechanism is connected with a sample outlet shifting fork, the sample outlet shifting fork can be connected after the sample frame moves along with the second driving mechanism, and the sample frame can horizontally move relative to the bottom plate of the sample frame under the action of the third driving mechanism.

3. The dry fluoroimmunoassay analyzer for realizing multi-channel and automatic detection according to claim 2, wherein: the shaking-up module comprises a supporting mechanism, the supporting mechanism is fixed with the rack, a shaking-up main body and a driving mechanism IV are arranged on the supporting mechanism, the driving mechanism IV is fixed with the supporting mechanism, the shaking-up main body is simultaneously connected with the driving mechanism IV and the supporting mechanism, the shaking-up main body can rotate around the joint of the supporting mechanism under the driving of the driving mechanism IV, and the sample rack and the cap pulling-up module are movably connected with the shaking-up main body; shake even main part below and be provided with clamping mechanism and actuating mechanism five, and clamping mechanism can move the relatively supporting mechanism and realize pressing from both sides tightly or loosen the action under actuating mechanism five drives, and clamping mechanism is including shaking even holder, briquetting and linear guide five, and shake even holder and linear guide five and connect, and linear guide five is fixed on supporting mechanism, and the briquetting is fixed with shaking even holder, and the briquetting with shake elastic connection between the even main part, shake even holder and can move along linear guide five, shake even holder and actuating mechanism five and connect, actuating mechanism five is connected with and presss from both sides tight mounting panel, is provided with the gyro wheel on pressing from both sides tight mounting panel, presss from both sides tight mounting panel and makes the gyro wheel get into the briquetting and shake even holder contact with shaking even holder after between the holder under actuating mechanism five effects linear motion.

4. The dry fluoroimmunoassay analyzer for realizing multi-channel and automatic detection according to claim 3, wherein: the cap pulling module comprises a transverse plate connecting frame, the transverse plate connecting frame is fixed with the rack, a moving mechanism and a plurality of clamping jaws are mounted on the transverse plate connecting frame, the clamping jaws are connected with the moving mechanism and can move relative to the transverse plate connecting frame under the driving of the moving mechanism, and the clamping jaws are movably connected with the shaking main body and the sample rack; the transverse plate connecting frame is provided with a clamping mechanism, the clamping mechanisms are connected with the clamping jaws, and the clamping jaws are clamped or loosened under the action of the clamping mechanisms; the transverse plate connecting frame is provided with a rotating mechanism, the rotating mechanism is connected with the clamping jaw, and the clamping jaw rotates around the axis of the rotating mechanism.

5. The dry fluoroimmunoassay analyzer for realizing multi-channel and automatic detection according to claim 4, wherein: the suction head module comprises a suction head box mounting plate, the suction head box mounting plate is fixed with the rack, a suction head box is arranged on the suction head box mounting plate, a driving mechanism six is arranged on the suction head box mounting plate, the driving mechanism six is connected with the suction head box, the suction head box can move relative to the suction head box mounting plate under the action of the driving mechanism six, and the suction head box is connected with the sample adding module in the moving process; be provided with actuating mechanism seven on the suction head box mounting panel, actuating mechanism seven is connected with punctures the head, punctures the head and vertically removes under actuating mechanism seven effect.

6. The dry fluoroimmunoassay analyzer for realizing multi-channel and automatic detection according to claim 5, wherein: the sample adding module comprises a sample adding stand column, the sample adding stand column is fixed with the rack, a first sample adding transverse plate is arranged between the sample adding stand columns, the first sample adding transverse plate is fixed with the sample adding stand column, a second sample adding transverse plate is arranged between the first sample adding transverse plate, the second sample adding transverse plate can move along the first sample adding transverse plate, a gun can be arranged on the second sample adding transverse plate, the gun can move along the second sample adding transverse plate, and meanwhile the gun can move in the vertical direction and can be inserted into a suction head box or a shaking uniform main body or a sample frame.

7. The dry fluoroimmunoassay analyzer for realizing multi-channel and automatic detection according to claim 1, wherein: the incubation module comprises an incubation data acquisition mechanism and a waste card strip removing mechanism, the transportation module can relatively incubate the data acquisition mechanism and remove the waste card strip removing mechanism, the transportation module can be movably connected with the incubation data acquisition mechanism and the waste card strip removing mechanism, a heating plate and a temperature sensor are arranged on the incubation data acquisition mechanism, and the temperature sensor is connected with the heating plate.

8. The dry fluoroimmunoassay analyzer for realizing multi-channel and automatic detection according to claim 7, wherein: the reagent card box module comprises a card box device, a scanning device is arranged on the card box device, and the scanning device can move relative to the card box device; the card box device is externally provided with a card hooking mechanism which can move relative to the card box device and can be inserted into the card box device.

9. The dry fluoroimmunoassay analyzer for realizing multi-channel and automatic detection according to claim 8, wherein: the transportation module comprises a transportation mechanism and an eight driving mechanism, the eight driving mechanism is connected with the transportation mechanism, and the eight driving mechanism can drive the transportation mechanism to move horizontally.

10. The dry fluoroimmunoassay analyzer for realizing multi-channel and automated detection according to claim 9, wherein: the conveying mechanism comprises a first conveying plate, the first conveying plate is connected with an eighth driving mechanism and can horizontally move under the action of the eighth driving mechanism, a seventeenth driving mechanism and a second conveying plate are arranged on the first conveying plate, the seventeenth driving mechanism is connected with the second conveying plate, and the second conveying plate horizontally moves relative to the first conveying plate under the action of the seventeenth driving mechanism; the conveying plate II is provided with a conveying shifting fork and a driving mechanism nine, the driving mechanism nine is installed on the conveying plate II and connected with the driving mechanism nine, and the conveying shifting fork can vertically move under the action of the driving mechanism nine and is connected with the hook clamping mechanism.