CN218412570U - Full-automatic chemiluminescence determinator - Google Patents

Abstract

The utility model relates to a full-automatic chemiluminescence apparatus, including waste material box module, reagent processing module, scanning module, sample processing module, washing liquid and waste liquid bucket, belt cleaning device module, reaction module, mixing module, reading module, TIP head module, incubation module, three-dimensional arm module, move liquid device. The utility model discloses a three-dimensional arm adds liquid-transfering device and replaces manipulator or grab the clamp, shift, get and put reaction cup, adds the first sample application needle of replacement of cooperation TIP and come the application of sample and add the reagent, has avoided cross contamination.

Description

Full-automatic chemiluminescence determinator

Technical Field

The utility model belongs to the technical field of chemiluminescence immunoassay, a full-automatic chemiluminescence apparatus is related to.

Background

The chemiluminescence immunoassay technology combines a chemiluminescence immunoassay technology with high sensitivity with high specificity immunoreaction, is used for detecting and analyzing various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins, medicaments and the like, and is a latest immunoassay technology developed after radioimmunoassay, enzyme immunoassay, fluorescence immunoassay and time-resolved fluorescence immunoassay. It integrates high sensitivity, high specificity, high automation and no radioactive pollution into one body, and can raise the microanalysis by one order of magnitude.

Most full-automatic chemiluminescence analyzers in the market at present comprise a manipulator or a chain reaction tube conveying mechanism and essential component modules of a sample needle and a reagent needle. The technical scheme is determined according to the principle of chemiluminescence and a reaction detection step, firstly, serum of a sample to be detected is added into a reaction tube through a sample needle, then a reaction reagent is extracted through a reagent needle and added into the reaction tube for reaction, then the reaction tube is moved into a reaction module for reaction through a manipulator or a chain conveyor belt, after the reaction is finished, the reaction tube needs to be continuously transferred to a cleaning module for cleaning and adsorbing magnetic beads, and finally, detection is carried out.

In the whole reaction process, the sample and the reagent in the reaction tube need to be extracted and added by using a needle, and the movement of the reaction tube needs to be completed by a manipulator or a chain transmission mechanism. Both of these two methods have the defects that the former needle sample adding has a cleaning problem, a complete set of cleaning device is needed, including a pump, a pipeline, a cleaning pool, waste liquid recovery and the like, even if the risk of cross contamination exists, the manipulator or the chain transmission has the defects of complex structure and high failure rate.

In view of the prior art, it is very useful and promising to develop a chemiluminescence apparatus that can avoid the above defects.

SUMMERY OF THE UTILITY MODEL

The utility model provides a full-automatic chemiluminescence determinator,

the method comprises the following steps: (1) a three-dimensional arm module: the device is used for providing movement for transferring reagents, samples and reaction cups;

(2) A liquid transfer device: the reagent and the sample are sucked, transferred and uniformly mixed;

(3) A TIP head module: placing a TIP head;

(4) A scanning module: reading the test strip information;

(5) A sample processing module: through the use of the sample rack, a sample tube which can be supported is placed;

(6) A reagent processing module: the magnetic bead mixing mechanism is responsible for reagent refrigeration, reagent storage and reagent mixing;

(7) An incubation module: the reaction cup is used for placing the reaction cup and providing an incubation reaction environment for the reagent in the reaction cup;

(8) A reaction module: the device is used for testing the reaction of each reagent and sample in the reaction process;

(9) A cleaning device module: cleaning the magnetic beads;

(10) A blending module: uniformly mixing the reagents;

(11) A reading module: detecting the luminous value of the reaction, and outputting the luminous value to a computer terminal for result conversion;

(12) Cleaning liquid and waste liquid bucket: placing a cleaning solution and a waste liquid;

(13) A waste box module: load used TIP heads and reaction cups.

The utility model discloses in, a full-automatic chemiluminescence apparatus, include: through the support column

Set up in the waste material box module in the bottom plate outside, reagent processing module, scanning module, sample processing module, washing liquid and waste liquid bucket, set up in the inboard belt cleaning device module of bottom plate, reaction module, mixing module, reading module, TIP head module, incubation module and set up in the three-dimensional arm module of bottom plate instrument inboard top, three-dimensional arm module both ends are fixed in the frame upper portion of bottom plate both sides through three-dimensional arm mount, and the frame outside covers there is the safety cover.

The utility model discloses in, three-dimensional arm module includes three-dimensional arm mount, sets up X axle motion subassembly, setting on the three-dimensional arm mount are in Y axle motion subassembly, setting on the X axle motion subassembly are in Z axle motion subassembly on the Y axle motion subassembly, wherein Y axle supporting plate in the Y axle motion subassembly sets up on X axle slider of X axle motion subassembly Z axle supporting plate sets up on Y axle slider of Y axle motion subassembly, Z axle slider in the Z axle motion subassembly is provided with the pipettor mounting panel, be provided with on the pipettor mounting panel and move the liquid device, wherein X axle motion subassembly drives the liquid device and does the side-to-side motion, and Y axle motion subassembly drives the liquid device and does the seesaw, and Z axle motion subassembly drives the liquid device and does the up-and-down motion.

The utility model discloses in, the TIP head module includes support column IV, TIP head, supporting seat and TIP head box, the supporting seat is fixed in on four support columns IV, the supporting seat top is provided with TIP head box, the first box loading of TIP has the TIP head, the TIP head includes TIP head top tube, TIP head low tube and suction head part, and TIP head mouth internal dimension is unanimous with the inside size of the reaction cup mouth of pipe, and the size can match the pressure head of pipetting device anterior segment, and the TIP head can be realized snatching through pipetting device disect insertion.

The utility model discloses in, reagent processing module includes reagent box frame, mixing motor I, kit tray, refrigerating plant, wherein establishes reagent bottle and magnetic bead bottle in the reagent box frame, and the magnetic bead bottle sets up in reagent box rest base portion one side, and magnetic bead bottle bottom has the gear, with the mixing rack cooperation among the mixing motor I. The driving wheel of the mixing motor I drives the mixing rack to do linear reciprocating motion, and the mixing rack does linear reciprocating motion to drive the magnetic bead bottle to rotate ceaselessly. There is the magnetic bead in the magnetic bead bottle, and the magnetic bead bottle need be ceaselessly rotatory when using to guarantee that the magnetic bead in the bottle does not deposit, reaches the purpose of magnetic bead mixing, refrigerating plant is located reagent box frame bottom.

The utility model discloses in, hatching the module and including reaction cup, reaction glass stand, incubation seat, temperature detect switch, temperature sensor, support column II and heating film. The incubation seat is fixed on four support pillars II, a reaction cup holder is arranged on the incubation seat and is loaded with a reaction cup, a heating film is arranged at the bottom of the incubation seat, temperature detect switch, temperature sensor set up in heating the membrane side, are fixed in the whippletree that two II tops of support column are connected, heat for the incubation seat through heating the membrane, ensure the required temperature environment of reagent reaction. The temperature sensor transmits the current temperature in real time to be displayed on a screen, and when the set temperature is too high, the temperature control switch is automatically switched off.

In the utility model, the reaction module and the cleaning device module are arranged on a support column I, and a cleaning needle is arranged on a needle frame; the outer part of the cleaning needle is sleeved with a mixing auxiliary driving wheel; a mixing driving wheel is fixed at the upper end of a mixing driving motor of the cleaning device module, the mixing driving wheel is connected with a mixing auxiliary wheel through a mixing connecting belt, and a cup grabbing clamping sleeve is connected below the mixing auxiliary wheel; a needle frame motion driving wheel is fixed at the front section of the needle frame motion motor, the needle frame motion driving wheel is connected with the needle frame motion auxiliary wheel through a needle frame motion synchronous belt, the needle frame motion auxiliary wheel can be driven to move up and down through the guiding of a left guide supporting module and a right guide supporting module in a matched manner by a lead screw motor, so that a reaction cup on a reaction turntable can be conveniently grabbed and pushed down, a cup grabbing clamping sleeve can be driven by the mixing driving motor to grab the reaction cup through the connection relationship above and then rotate rapidly, and the rotation direction of the mixing driving motor can be controlled by software to drive samples, reagents and magnetic beads in the reaction cup to be mixed; after uniform mixing, the magnetic separation can be better carried out through the magnetic part, and unreacted free impurities can be separated by cleaning through the cleaning liquid and pumping away the waste liquid through the cleaning needle. The reaction turntable is connected with a reaction turntable auxiliary wheel through a connecting shaft and a bearing, a reaction turntable driving wheel is fixed at the upper end of the reaction turntable movement motor, the reaction turntable driving wheel is connected with the reaction turntable auxiliary wheel through a reaction turntable synchronous belt, and the reaction turntable can be driven by the reaction turntable movement motor to rotate through the connection relation, so that the reaction cup on each station can be accurately stopped at a required position to facilitate the pipelined cleaning and uniformly mixing magnetic separation. A liquid discharge pipe moving motor is installed beside the support column I, a liquid discharge pipe moving driving wheel is fixed on the front section of the liquid discharge pipe moving motor, a liquid discharge pipe moving auxiliary wheel at the other end is connected through a liquid discharge pipe moving synchronous belt, the liquid discharge pipe is fixed on the liquid discharge pipe moving synchronous belt, as shown in figure 16, a reaction cup is of a structure with a step surface in a thick upper part and a thin lower part, the reaction cup is of a hollow structure in the middle of a reaction cup loading hole, the reaction cup is placed on the reaction turntable through the step surface, the needle and the pipeline need to be cleaned after the instrument is started to initialize, the liquid discharge pipe can be inserted into the reaction turntable hole upwards through the connection relation, waste liquid discharged by the cleaning needle is received, and the inner wall of the cleaning needle and the inner wall of the pipeline are convenient to reduce cross contamination.

The utility model discloses in, the mixing module includes mixing carousel, mixing motor II, mixing main wheel, mixing stand, mixing hold-in range, mixing auxiliary wheel and mixing eccentric wheel, drives through three-dimensional arm module and moves liquid device and shift to the downthehole of mixing carousel with the reaction cup. The mixing main wheel is installed in II belows of mixing motor, drives the mixing main wheel under the drive of mixing motor II, and the mixing main wheel passes through the mixing hold-in range with the mixing auxiliary wheel to be connected, and the mixing auxiliary wheel is connected through the bearing on the mixing eccentric wheel cover with the mixing carousel, and mixing motor II realizes through above-mentioned relation of connection that just reversing can drive the mixing carousel and vibrate like this to can drive reagent in the reaction cup, sample, substrate, the abundant mixing of magnetic particle, three-dimensional arm module drive move liquid device take the reaction cup cover to read the value in the reading module behind the mixing.

In the utility model, the reading module comprises a bottom plate, a support column III arranged on the bottom plate and a mounting plate arranged on the support column III; the device comprises a mounting plate, a reaction cup placing and placing device and a detection plate, wherein a light shield supporting column and a detection plate are arranged on the mounting plate, a light shield is arranged on the light shield supporting column, a reaction cup taking hole is formed in the light shield corresponding to the position of a reaction cup marked with a sequence number on a picture, the light shield is fixedly pressed on light shielding foam through the light shield supporting column, the environment of placing and placing the reaction cup Kong Anshi is shielded through a shutter baffle, a rotary electromagnet is arranged on one side of the detection plate, the upper end of the rotary electromagnet is fixedly provided with the shutter baffle, a photomultiplier is arranged on the other side of the detection plate, and 4 detection plate holes are uniformly distributed on the detection plate; the detection disc is driven by a detection disc motor arranged on the mounting plate to work. The output end of the detection disc motor is connected with the driven belt wheel through the driving belt wheel, the connecting synchronous belt and the driven belt wheel in sequence. The working principle is as follows: after a special reagent for detecting items is added to the reaction cup through other modules in the whole chemiluminescence flow, and magnetic beads are added, the reaction cup is taken out by a three-dimensional arm gripper and then moved to the position right above the reaction cup, after a shutter baffle is driven to open by the counterclockwise rotation of a rotary electromagnet, the reaction cup is placed into a detection plate hole in a detection plate through the reaction cup taking and placing hole by the three-dimensional arm gripper, the three-dimensional arm gripper exits from the reaction cup, the rotary electromagnet rotates clockwise to drive the shutter baffle to stay right above the reaction cup, a detection plate motor controls the rotation position and the rotation angle through a photoelectric switch, the detection plate is driven to rotate two hole positions, so that a side hole can be aligned with a photomultiplier detection hole, the numerical value of the luminous value of the reagent in the reaction cup can be directly read, the photomultiplier detects the luminous value of the reagent, outputs the luminous value to a PC end, and converts the result and outputs the detection result. The detection plate rotates the angle of the two hole positions again to bring the reaction cup with the read value back to the position of the reaction cup taking and placing hole, then the rotary electromagnet rotates anticlockwise to drive the shutter baffle to return to the initial position, and then the reaction cup is taken out of the position of the reaction cup taking and placing hole by the manipulator and is placed in the waste material box.

The utility model discloses in, washing liquid and waste material bucket are the waste liquid that is retrieved after being used for splendid attire washing module required washing liquid and clear liquid.

In the utility model, the waste material box module comprises a waste material box, a waste material box bearing frame, a slide block, a conveyor belt and a motor; the waste material box can load used TIP heads and reaction cups, and the motor drives the waste material box bearing frame to move back and forth through the conveying belt and the sliding block, so that the used TIP heads and the reaction cups are uniformly shaken, and accumulation is prevented.

The utility model has the advantages that: this scheme uses three-dimensional arm to add the liquid-transfering device and replaces manipulator or grab the clamp, shift, get and put the reaction cup, adds and cooperates the TIP head to replace the application of sample needle and come the application of sample and add the reagent, has avoided cross contamination, and the error rate of this kind of mode will be less than the manipulator scheme greatly, has improved the precision and the accuracy of detection, adds the mixing module in addition, can make reagent, sample, substrate, the abundant mixing of magnetic particle in the reaction cup, makes the reaction system more even stable, reduces the testing result deviation.

Drawings

(1) FIG. 1 is a diagram: the utility model discloses the structure chart

1. A three-dimensional arm module; 2. a cleaning device module; 3. a reaction module; 4. an incubation module; 5. a cleaning solution and a waste liquid barrel; 6. a sample processing module; 7. a scanning module; 8. a reagent processing module; 9. a waste box module; 10. a TIP head module; 11. a value reading module; 12. a pipetting device; 13. blending module

(2) FIG. 2 is a diagram of: test workflow diagram

(3) FIG. 3 is a diagram: schematic perspective view of sample processing module

31. A sample pipe frame; 32. a sample rack box; 33. sensor with a sensor element

(4) FIG. 4 is a diagram of: reagent processing module perspective schematic view

41. A reagent box holder; 42. a reagent bottle; 43. a magnetic bead bottle; 44. mixing the racks uniformly; 45. a driving wheel; 46. uniformly mixing a motor I; 47. a kit tray; 48 refrigerating device

(5) Fig. 5 and 6 show: reaction module and cleaning device module schematic perspective view

201. A needle frame; 150. cleaning the needle; 151. uniformly mixing the auxiliary driving wheels; 152. a screw motor; 153. a cup grabbing and clamping sleeve; 154. a reaction cup; 155. a reaction turntable; 156. a liquid discharge pipe; 157. a reaction turntable auxiliary wheel; 158. a liquid discharge pipe motion auxiliary wheel; 159. a support pillar I; 160. a liquid discharge pipe moves to synchronize the belt; 161. a drain motion motor; 162. a liquid discharge pipe movement driving wheel, 163 and a needle frame movement motor; 164. a needle frame motion driving wheel; 165. a needle frame motion synchronous belt; 166. a reaction turntable movement motor; 167. a reaction turntable synchronous belt; 168. a reaction turntable driving wheel; 169. the needle frame moves the auxiliary wheel; 170. a magnetic member; 171. a guide support module; 172. uniformly mixing a driving motor; 173. uniformly mixing the connecting bands; 174. mixing driving wheel

(6) FIG. 7 is a diagram of: schematic perspective view of incubation module

154. A reaction cup; 61. a reaction cup holder; 62. an incubation seat; 63. a temperature control switch; 64. a temperature sensor; 65. a support pillar II; 66. heating film

(7) Fig. 8 is, fig. 9: reading module three-dimensional schematic diagram

67. A photomultiplier tube; 68. detecting a disc; 69. a shutter plate; 70. the rotary electromagnet, 71 and a lens hood support column; 72. detecting a disc motor; 73. mounting a plate; 74. a support column III; 75. a driving pulley; 76. synchronous belt 77, driven pulley; 78. a photosensor; 79. a light shield; 7901. a reaction cup taking hole; 80. shading foam

(8) FIG. 10 is a diagram: three-dimensional schematic diagram of TIP head module

81. A support pillar IV; 82. a TIP header; 8201. a TIP head upper pipe; 8202. TIP head down tube; 8203. a suction head; 83. a supporting seat; 84. TIP head box

(9) FIG. 11 is a diagram of: TIP head module stereo enlarged partial schematic diagram

85. Positioning a pin; 86. a limiting elastic sheet; 87. bottom plate platform

(10) FIG. 12 is a diagram: waste box module perspective view

91. A waste material box; 92. a waste bin carrier; 93. a slider; 94. a conveyor belt; 95. electric machine

(11) Fig. 13, 14, 15 are: three-dimensional arm module perspective view

100-X axis motion component, 200-Y axis motion component, 300-Z axis motion component, 400-mute slide rail component, 101-first driving motor, 102-three-dimensional arm fixing frame, 103-bottom plate, 104-drag chain plate supporting block I, 105-drag chain plate, 106-parallel slide rail I, 107-drag chain, 108-drag chain plate supporting block II, 109-first synchronous pulley I, 110-first synchronous belt, 111-X axis sliding block, 112-first synchronous pulley II, 113-baffle plate, 114-first limit optical coupler and 115-parallel slide rail II; 201-a second driving motor, 202-a second synchronous pulley I, 203-a second limit optical coupler, 204-a blocking plate, 205-a Y-axis supporting plate, 206-a sliding rail, 207-a second synchronous belt, 208-a second synchronous pulley II, 209-a drag chain supporting plate, 210-a drag chain, 211-a Y-axis sliding block, 301-a third driving motor, 302-a motor fixing plate, 303-a mounting plate, 304-a third synchronous pulley I, 305-a third limit optical coupler, 306-a blocking plate, 307-a Z-axis sliding block, 308-a Z-axis supporting plate, 309-a rib plate, 310-a pipette mounting plate, 312-a sliding rail, 311-a third synchronous belt, 313-a pipette device, 314-a third synchronous pulley II, 401-an optical axis, 402-a linear sliding shaft sleeve and 403-an optical axis mounting plate.

(12) FIG. 16 is a graph showing: blending module perspective view

50-mixing turntable, 51-mixing motors II and 52-mixing main wheel, 53-mixing upright post, 54-mixing synchronous belt, 55-mixing auxiliary wheel and 56-mixing eccentric wheel

(13) FIG. 17 is a graph of: enlarged schematic view of reaction cup

154-reaction cup

(14) FIG. 18 is a graph of: TIP head enlarged schematic diagram

82-TIP head, 8201-TIP head upper tube, 8202-TIP head lower tube, 8203-suction head

Detailed Description

Example 1: the utility model provides a pair of detection operation flow of full-automatic chemiluminescence determinator

(1) Selecting an item to be tested on a screen; the sample tube rack 31 has 40 sample positions, and can perform single or multiple sample simultaneous tests. The test items can be selected from single or multiple items in different combinations, and the positions of the test samples have no interference influence;

(2) Manually placing a sample at the position of a sample rack, then placing a kit at the kit bearing position in the instrument, placing a TIP head on the TIP head module, placing a reaction cup on the incubation module, closing the door, and selecting a project to click to start testing;

(3) The testing process is full-automatic, and the testing process comprises the following steps: reading test strip information; automatically taking a TIP head; incubating the reagent; mixing the reagents (mixing in a sucking and spitting mode); separating magnetic particles; shaking and mixing uniformly; moving the reaction cup to a value reading module, and reading a luminous value; automatically removing the TIP head; and outputting a test result.

(4) As shown in fig. 1, the utility model discloses a full-automatic chemiluminescence apparatus comprises three-dimensional arm module 1, belt cleaning device module 2, reaction module 3, incubation module 4, waste liquid bucket 5, sample processing module 6, scanning module 7, reagent processing module 8, waste material box module 9, TIP head module 10, reading module 11, liquid-transfering device 12, mixing module 13 to and electrical control part and computer control end.

(5) As shown in FIG. 1, the three-dimensional arm module 1 can move the pipetting device freely on the working plane, the reaction cup 154 uses the TIP head 82 to add the sample and reagent, and the reaction cup 154 is picked up and moved by the pipetting device 12.

(6) Fig. 3 shows a sample processing module 6, which is composed of a sample tube rack 31, a sample tube rack cassette 32, and a sensor 33. Each time the sample tube rack 31 is inserted into the sample tube rack box 32, the sensor 33 will detect the insertion signal and transmit it to the computer control end, so that the instrument can grasp the information of the sample on the rack at any time.

(7) Fig. 4 shows a reagent processing module 8, which is composed of a reagent box frame 41, a blending rack 44, a driving wheel 45, a blending motor i 46, a reagent box tray 47 and a refrigerating device 48. The reagent cassette rack 41 contains a reagent bottle 42 and a magnetic bead bottle 43. The magnetic bead bottle sets up in reagent box frame bottom one side, and it need be ceaselessly rotatory when using to guarantee that the magnetic bead in the bottle does not deposit, and magnetic bead bottle 43 bottom has the gear, and with mixing rack 44 cooperation, drive mixing rack 44 through the drive wheel 45 of mixing motor I46 and be linear reciprocating motion, keep the magnetic bead bottle rotatory incessantly, reach the purpose of magnetic bead mixing. The cooling device 48 can provide a longer storage time for the reagent in the cassette rack 41 to maintain a constant temperature.

(8) FIGS. 5 and 6 show a reaction module 3 and a cleaning device module 2, which are mounted on a support column I159, and a cleaning needle 150 is mounted on a needle holder 201 of the cleaning device module 2; a uniformly mixing auxiliary wheel 151 is sleeved outside the cleaning needle 150; a blending driving wheel 174 is fixed at the upper end of a blending driving motor 172 of the cleaning device module, the blending driving wheel 174 is connected with a blending auxiliary wheel 151 through a blending connecting belt 173, and a cup grabbing clamping sleeve 153 is connected below the blending auxiliary wheel 151; a needle frame motion driving wheel 164 is fixed at the front section of the needle frame motion motor 163, the needle frame motion driving wheel 164 is connected with a needle frame motion auxiliary wheel 169 through a needle frame motion synchronous belt 165, the whole needle frame 201 can be driven to move up and down through the guiding of a left guide supporting module 171 and a right guide supporting module 152 in a matching manner so as to conveniently grab and push down a reaction cup 154 on a reaction turntable 155, the mixing driving motor 172 can drive a cup grabbing clamping sleeve 153 to grab the reaction cup 154 through the connection relationship above and then rotate rapidly, and the rotation direction of the mixing driving motor 172 is controlled through software so as to drive samples, reagents and magnetic beads in the reaction cup 154 to be mixed uniformly; after uniform mixing, the magnetic separation can be better carried out through the magnetic part 170, and unreacted free impurities can be separated through cleaning by the cleaning liquid and the waste liquid pumped by the cleaning needle 150. The reaction turntable 155 is connected with a reaction turntable auxiliary wheel 157 through a connecting shaft and a bearing, the upper end of a reaction turntable movement motor 166 is fixed with a reaction turntable driving wheel 168, the reaction turntable driving wheel 168 is connected with the reaction turntable auxiliary wheel 157 through a reaction turntable synchronous belt 167, and the reaction turntable 155 can be driven to rotate by the reaction turntable movement motor 166 through the connection relation, so that the reaction cup 154 on each station can be accurately stopped at a required position to facilitate the pipelined cleaning, uniform mixing and magnetic separation. A drain pipe movement motor 161 is installed beside the support column I159, a drain pipe movement driving wheel 162 is fixed at the front section of the drain pipe movement motor 161, the drain pipe movement driving wheel is connected with a drain pipe movement auxiliary wheel 158 at the other end through a drain pipe movement synchronous belt 160, the drain pipe 156 is fixed on the drain pipe movement synchronous belt 160, as shown in FIG. 16, the reaction cup 154 is in a structure with a step surface from top to bottom, the middle of a hole position for loading the reaction cup on the reaction turntable 155 is in a hollow structure, the reaction cup 154 is placed on the reaction turntable 155 through the step surface, the design can ensure that the needle 150 and the pipeline need to be cleaned after the instrument is started up and initialized, and the drain pipe 156 can be upwards inserted into the hole of the reaction turntable 155 through the connection relationship to receive waste liquid discharged by the cleaning needle 150, so as to reduce cross contamination of the inner walls of the cleaning needle and the pipeline.

(9) FIG. 7 shows an incubation module 4, which is composed of a reaction cup 154, a reaction cup holder 61, an incubation seat 62, a temperature control switch 63, a temperature sensor 64, a support post II 65 and a heating film 66. The incubation seat is fixed on four support columns II 65, be provided with reaction cup holder 61 on the incubation seat 62, reaction cup 154 is loaded to reaction cup holder 61, incubation seat 62 bottom is provided with heating film 66, temperature switch 63, temperature sensor 64 set up in heating film 66 side, are fixed in on the horizontal bar that two support columns II 65 top are connected. The temperature environment required for the reagent reaction is ensured by heating the incubation seat 62 through the heating film 66. The temperature sensor 64 transmits the current temperature in real time to be displayed on a screen, and when the set temperature is too high, the temperature control switch 63 is automatically switched off.

(10) Fig. 8 and 9 show a value reading module 11, which includes a support column iii 74 disposed on a base plate, and a mounting plate 73 disposed on the support column iii 74; a light shield support column 71 and a detection disc 68 are arranged on the mounting plate 73, a light shield 79 is arranged on the light shield support column 71, a reaction cup taking and placing hole 7901 is formed in the light shield 79 and corresponds to the position of the reaction cup 154 marked with a sequence number in the drawing, the light shield 79 is fixedly pressed on the light shield foam 80 through the light shield support column 71 and is shielded into a darkroom environment of the reaction cup taking and placing hole 7901 through a shutter baffle 69, a rotary electromagnet 70 is arranged on one side of the detection disc 68, the shutter baffle 69 is fixed at the upper end of the rotary electromagnet 70, a photomultiplier 67 is arranged on the other side of the detection disc 68, and 4 detection disc holes are uniformly distributed in the detection disc 68; the test tray 68 is driven by a test tray motor 72 disposed on the mounting plate. The output end of the detection disc motor 72 is connected with a driving pulley 75, a connection synchronous belt 76 and a driven pulley 77 in sequence. The working principle is as follows: after the reaction cup 154 is added with a special reagent for a detection item by other modules in the whole chemiluminescence flow, and after magnetic beads are added, the reaction cup 154 is taken out by a three-dimensional arm gripper and then moved to the position right above the reaction cup 154, after the rotary electromagnet 70 rotates anticlockwise to drive the shutter baffle 69 to be opened, the reaction cup 154 is placed into a detection disc hole in the detection disc 68 by the three-dimensional arm gripper through the reaction cup taking and placing hole 7901, then the three-dimensional arm gripper exits from the reaction cup, at the moment, the rotary electromagnet 70 rotates clockwise to drive the shutter baffle 69 to stay right above the reaction cup 154, the detection disc motor 72 sequentially passes through the driving pulley 75, the synchronous belt 76 and the driven pulley 77, the rotation position and angle are controlled by the photoelectric switch to drive the detection disc 168 to rotate two side holes so that the hole positions can be aligned with the detection hole of the photomultiplier 67 to directly read the value of the luminescence value of the reagent in the reaction cup 154, the photomultiplier 67 detects the luminescence value of the reagent, outputs the luminescence value to the PC end for result conversion, and outputs the detection result. The detection plate 68 rotates again to the position of the cuvette taking and placing hole 7901 with the cuvette 154 having read values at the angle of the two hole positions, then the rotary electromagnet 70 rotates counterclockwise to drive the shutter baffle 69 to return to the initial position, and then the manipulator takes out the cuvette 154 at the position of the cuvette taking and placing hole 7901 and places the cuvette 154 in the waste material box 91.

(11) Fig. 10 shows a TIP head module 10, which is composed of a support post iv 81, a TIP head 82, a support seat 83, and a TIP head box 84. As shown in fig. 10, the supporting base 83 is fixed on four supporting columns iv 81, a TIP cartridge 84 is arranged on the top of the supporting base 83, the TIP cartridge 84 is loaded with a TIP 82, the TIP 82 includes a TIP upper tube 8201, a TIP lower tube and a 8202 suction head 8203, the internal size of the nozzle of the TIP 82 is consistent with the internal size of the nozzle of the reaction cup 154, the internal size can be matched with the pressure head at the front section of the pipetting device 12, the pipetting device 12 can be directly pressed in and inserted, the pipetting device 12 has a built-in motor drive to withdraw the TIP 82 and the reaction cup 154, thereby avoiding the use of an additional manipulator device to transport the reaction cup 154, and the TIP head module 10 facilitates the easy taking and placing of the whole-plate TIP cartridge 84, and the accurate limiting and no shaking facilitates the accurate insertion and grasping of the pipetting device 12, as shown in fig. 11 adopts a special design embodiment: the whole TIP head box 84 can be limited and pressed to guarantee that the position is accurate and does not shake by means of a left limiting elastic sheet 86, a right limiting elastic sheet 86 and a placing bottom plate platform 87 through a locating pin 85, so that the pipetting device 12 can be inserted and taken conveniently, the TIP head box 84 can be clamped by the limiting elastic sheet 86, and the TIP head 82 is taken up by the pipetting device 12 and does not move upwards and is not taken up.

(12) Fig. 12 shows a waste cassette module 9, which is composed of a waste cassette 91, a waste cassette carrier 92, a slide 93, a conveyor belt 94 and a motor 95. The waste material box 91 can be loaded with the used TIP head 82 and the reaction cup 154, the motor 95 drives the waste material box bearing frame 92 to move back and forth through the conveyor belt 94 and the slide block 93, so that the used TIP head 82 and the reaction cup 154 are shaken uniformly, and the waste material box 91 is preferably made of aluminum alloy.

(13) Fig. 13, 14, 15 are three-dimensional arm modules comprising: the three-dimensional arm fixing frame 102 and the X-axis motion assembly 100 comprise: the three-dimensional arm fixing frame comprises a bottom plate 103 arranged on the three-dimensional arm fixing frame 102, a parallel slide rail I106 and a parallel slide rail II 115 which are arranged on the bottom plate 103, a drag link plate I104 and a drag link plate II 108, drag link plates 105 arranged on the drag link plate I104 and the drag link plate II 108, drag links 107 arranged on the drag link plates 105, X-axis sliders 111 arranged on the drag link plates 105 and the parallel slide rail I106, blocking pieces 113 arranged on the X-axis sliders 111, first limiting optical couplers 114 arranged on the side edges of the bottom plate 103, a first driving motor 101 arranged at one end of the bottom plate 103, a first synchronous pulley I109 arranged on the first driving motor 101, a first synchronous pulley II 112 arranged at the other end of the bottom plate 103, and a first synchronous belt 110 which is connected with the first synchronous pulley I109 and the first synchronous pulley II 112 and is arranged in parallel with the parallel slide rail I106; the X-axis slider 111 is coupled to the first timing belt 110. The Y-axis motion assembly 200 includes: a Y-axis support plate 205, a tow chain support plate 209 disposed at one side of the Y-axis support plate 205, a tow chain 210 disposed on the tow chain support plate 209, a slide rail 206 disposed at the other side of the Y-axis support plate 205, a Y-axis slider 211 disposed on the slide rail 206, a stopper 204 disposed on the Y-axis slider 211, a second limit optocoupler 203 disposed at the other side of the Y-axis support plate 205, a second driving motor 201 disposed at the other side of the Y-axis support plate 205, a second synchronous pulley i 202 disposed on the second driving motor 201, a second synchronous pulley ii 208 disposed at the front of the other side of the Y-axis support plate 205, and a second synchronous belt 207 connecting the second synchronous pulley i 202 and the second synchronous pulley ii 208 and disposed in parallel with the slide rail 206; the Y-axis slider 211 is connected to the second timing belt 207. The Z-axis motion assembly 300 includes: a Z-axis support plate 308, a rib plate 309 disposed on one side of the Z-axis support plate 308, a mounting plate 303, a slide rail 312, and a third limit optical coupler 305 disposed on the other side of the Z-axis support plate 308, a Z-axis slider 307 disposed on the slide rail 312, a pipette mounting plate 310 disposed on the Z-axis slider 307, a pipette device 12 disposed on the pipette mounting plate 310, a motor fixing plate 302 disposed on the mounting plate 303, and a third synchronous pulley ii 314 disposed on the other end of the mounting plate 303, a third driving motor 301 disposed on the motor fixing plate 302, a third synchronous pulley i 304 disposed on the third driving motor 301, and a third synchronous belt 311 connecting the third synchronous pulley i 304 and the third synchronous pulley ii 314 and disposed in parallel with the slide rail 312; the Z-axis slider 312 is connected to the third timing belt 311. The silent slide rail assembly 400: the optical axis mounting device comprises a linear sliding shaft sleeve 402, an optical axis 401 arranged on the linear sliding shaft sleeve 402, and an optical axis mounting plate 403 arranged at one end of the optical axis. Through set up Y axle motion subassembly on X axle motion subassembly to set up Z axle motion subassembly on Y axle motion subassembly, make and establish executive component on Z axle motion subassembly move liquid device 12 and can accomplish the front and back in the space, control and the removal of upper and lower direction and get TIP head and reaction cup in the cover, the TIP head is the same with reaction cup upper end inner diameter size, make things convenient for like this to move liquid device 12 cover and get TIP head imbibition and automatic withdrawing, also can realize the cover and get reaction cup and realize shifting and withdraw appointed station.

(14) Fig. 16 shows a blending module 13, which includes a blending turntable 50, a blending motor ii 51, a blending main wheel 52, a blending column 53, a blending synchronous belt 54, a blending auxiliary wheel 55 and a blending eccentric wheel 56, and the three-dimensional arm module 1 drives the pipetting device 12 to transfer a reaction cup 154 into a hole of the blending turntable 50. The blending main wheel 52 is arranged below a blending motor II 51, the blending main wheel 52 is driven by the blending motor II 51, the blending main wheel 52 is connected with a blending auxiliary wheel 55 through a blending synchronous belt 54, the blending auxiliary wheel 55 and the blending rotary disc 50 are connected through a bearing sleeved on a blending eccentric wheel 56, so that the blending motor II 51 realizes positive and negative rotation through the connection relation and can drive the blending rotary disc 50 to vibrate, reagents, samples, substrates and magnetic particles in the reaction cups 154 can be driven to be fully blended, and the three-dimensional arm module 1 drives the liquid transfer device 12 to sleeve the reaction cups 154 to read the values in the reading module 11 after blending.

(15) The three-dimensional arm module 1, the cleaning device module 2, the reaction module 3, the incubation module 4, the cleaning solution and waste liquid barrel 5, the sample processing module 6, the scanning module 7, the reagent processing module 8, the waste material box module 9, the TIP head module 10, the reading module 11, the liquid transfer device 12 and the blending module 13 are arranged in the rack.

(16) The working principle is as follows: the three-dimensional motion arm composed of the X axis, the Y axis and the Z axis can drive the pipetting device 12 to move on the working plane at will. For example, if the pipetting device 12 is to extract the cuvettes 154 from the incubation module 4 to the detection tray 68, the pipetting device 12 is first moved in the x-axis and the y-axis above the position coordinates of one of the cuvettes 154 in the incubation module 4, and then moved up and down in the Z-axis. The pipetting device 12 is inserted into the reaction cup 154 and lifted up, then the X-axis and the Y-axis move to the coordinate position of the detection plate 68, the Z-axis drives the pipetting device 12 to move downwards and place the pipetting device into the detection plate 68, and then the pipetting device 12 is provided with an ejection mechanism to unload the reaction cup 154 from the top, thereby completing the whole picking and placing actions. Extracting reaction cup 154 from incubation module 4 to reaction tray 56; pressing the sleeved TIP head 82 into the TIP head module 10, sucking the reagent from the reagent processing module 8 and spitting the reagent into the reaction cup 154; in accordance with the above-described procedure.

The above description is only for the preferred embodiment of the present invention and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (8)

1. A fully automated chemiluminescent assay meter comprising: the device comprises a waste box module, a reagent processing module, a scanning module, a sample processing module, a cleaning solution and a waste liquid barrel which are arranged on the outer side of a bottom plate through supporting columns, a cleaning device module, a reaction module, a blending module, a reading module, a TIP (TIP-in-process) head module and an incubation module which are arranged on the inner side of the bottom plate, and a three-dimensional arm module arranged above the inner side of a bottom plate instrument; the three-dimensional arm module is characterized by comprising a three-dimensional arm fixing frame, an X-axis motion assembly, a Y-axis motion assembly and a Z-axis motion assembly; the X-axis movement assembly is arranged on the three-dimensional arm fixing frame, a Y-axis support plate in the Y-axis movement assembly is arranged on an X-axis sliding block of the X-axis movement assembly, a Z-axis support plate in the Z-axis movement assembly is arranged on a Y-axis sliding block of the Y-axis movement assembly, a pipettor mounting plate is arranged on a Z-axis sliding block in the Z-axis movement assembly, and a pipettor device is arranged on the pipettor mounting plate; the mixing module comprises a mixing turntable, a mixing motor II, a mixing main wheel, a mixing upright post, a mixing synchronous belt, a mixing auxiliary wheel and a mixing eccentric wheel, wherein the mixing main wheel is arranged below the mixing motor II, the mixing main wheel and the mixing auxiliary wheel are connected through the mixing synchronous belt, and the mixing auxiliary wheel and the mixing turntable are connected through a bearing on the mixing eccentric wheel sleeve.

2. The full-automatic chemiluminescence tester according to claim 1, wherein the TIP head module comprises four support columns IV, a TIP head, a support seat and a TIP head box, the support seat is fixed on the four support columns IV, the TIP head box is arranged at the top of the support seat, the TIP head box is loaded with the TIP head, the TIP head comprises a TIP head upper tube, a TIP head lower tube and a TIP part, the inner dimension of the TIP part of the TIP head is consistent with the inner dimension of the nozzle of the reaction cup, and the TIP part can be matched with a pressure head at the front section of the pipetting device.

3. The full-automatic chemiluminescence determinator according to claim 1, wherein the reagent processing module comprises a reagent box frame, a blending motor I, a reagent box tray and a refrigerating device, wherein a reagent bottle and a magnetic bead bottle are arranged in the reagent box frame, the magnetic bead bottle is arranged on one side of the bottom of the reagent box frame, a gear is arranged at the bottom of the magnetic bead bottle and is matched with a blending rack in the blending motor I, and the blending rack is driven to do linear reciprocating motion by a driving wheel of the blending motor I; the refrigerating device is positioned at the bottom of the reagent box frame.

4. The full-automatic chemiluminescence determinator of claim 1, wherein the reaction module and the cleaning device module are mounted on a support column I, and a cleaning needle is mounted on a needle frame of the cleaning device module; a uniformly mixing auxiliary driving wheel is sleeved outside the cleaning needle; the mixing driving device comprises a cleaning device module, and is characterized in that a mixing driving wheel is fixed at the upper end of a mixing driving motor of the cleaning device module, the mixing driving wheel and a mixing auxiliary wheel are connected through a mixing connecting belt, and a cup grabbing clamping sleeve is connected below the mixing auxiliary wheel.

5. The full-automatic chemiluminescence determinator according to claim 1, wherein the incubation module comprises a reaction cup, a reaction cup holder, an incubation seat, a temperature control switch, a temperature sensor, a support column II and a heating film; the incubation seat is fixed on the four support columns II, a reaction cup holder is arranged on the incubation seat and loaded with a reaction cup, and a heating film is arranged at the bottom of the incubation seat; the temperature control switch and the temperature sensor are arranged on the side surface of the heating film, are fixed in a cross bar connected with the tops of the two support columns II, and heat the incubation seat through the heating film to ensure a temperature environment required by reagent reaction; the temperature sensor transmits the current temperature in real time to be displayed on a screen.

6. The full-automatic chemiluminescence determinator according to claim 1, wherein the reading module comprises a bottom plate, a support column III arranged on the bottom plate, and a mounting plate arranged on the support column III; a light shield support column and a detection disc are arranged on the mounting plate, and a light shield is arranged on the light shield support column; a rotary electromagnet is arranged on one side of the detection disc, a shutter baffle is fixed at the upper end of the rotary electromagnet, and a photomultiplier is arranged on the other side of the detection disc; the detection plate is uniformly provided with 4 detection plate holes, and the detection plate is driven by a driving unit arranged on the mounting plate to work.

7. The full-automatic chemiluminescence analyzer according to claim 1, wherein the waste cartridge module comprises a waste cartridge, a waste cartridge carrier, a slide, a conveyor belt and a motor; the waste material box is loaded with the TIP head and the reaction cup, and the motor drives the waste material box bearing frame to move back and forth through the conveying belt and the sliding block.

8. The full-automatic chemiluminescence determinator of claim 1, wherein the three-dimensional arm module, the cleaning device module, the reaction module, the incubation module, the cleaning solution and waste liquid bucket, the sample processing module, the scanning module, the reagent processing module, the waste material box module, the TIP head module, the reading module, the pipetting device, and the blending module are mounted in a rack.

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