CN113567696A - Full-automatic chemiluminescence immunoassay device - Google Patents
Full-automatic chemiluminescence immunoassay device Download PDFInfo
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- CN113567696A CN113567696A CN202110845723.1A CN202110845723A CN113567696A CN 113567696 A CN113567696 A CN 113567696A CN 202110845723 A CN202110845723 A CN 202110845723A CN 113567696 A CN113567696 A CN 113567696A
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Abstract
The invention discloses a full-automatic chemiluminescence immunoassay device, wherein a rack is provided with a sample, a suction head storage module, a reagent reaction module, a sample adding and transporting module, a lighting module and a reagent cleaning and separating module, the sample and suction head storage module is arranged below the reagent reaction module, the reagent reaction module is arranged below the sample adding and transporting module, the lighting module and the reagent cleaning and separating module, and the reagent cleaning and separating module is arranged between the sample adding and transporting module and the lighting module. The invention realizes the automatic addition and removal of the suction head, the magnetic bar sleeve and the film breaking head; automatic sample adding and sample discharging are realized; the conductive suction head is matched with a liquid level detection system, the depth of the suction head inserted below the liquid level of a sample is accurately controlled, and the sample adding precision requirement of the whole device is guaranteed; the reagent strip adopts a sealing and packaging mode, so that the shelf life of the reagent is long, and the reagent is convenient to transport; the state of the suction head is monitored in real time, and when the suction head is in an abnormal state, the suction head is fed back in time, and corresponding correction is carried out if necessary, so that the whole device is more intelligent.
Description
Technical Field
The invention belongs to the technical field of biological detection, and particularly relates to a full-automatic chemiluminescence immunoassay device.
Background
The development of the chemiluminescence immunity technology which is started in the last 70 th century becomes a mature and advanced technology for detecting ultramicro active substances, and the application range is wide. The detection technology has the advantages of high sensitivity, strong specificity, low reagent price, stable reagent, long effective period (6-18 months), stable and rapid method, wide detection range, simple operation, high automation degree and the like. The existing market generally has a large chemiluminescent immunoassay analyzer packaged by a plurality of reagents, the analyzer occupies a large space and has high requirements on the sample amount, the reagents must be used within a specified time after being unsealed, and otherwise, the test result is inaccurate. This type of instrument is not suitable for hospitals with small sample volumes or for small town hospitals. Meanwhile, such large-scale instruments are complex to operate, operators can operate the instruments only through systematic training, and the instruments are not beneficial to popularization in small and medium hospitals with limited medical resources. Moreover, the cost of the large-scale instruments is very high, and the large-scale instruments are not borne by hospitals in villages and towns, and are not beneficial to the vigorous popularization of primary medical treatment. And there are less single reagent strip chemiluminescence immunoassay appearance in the existing market, and this kind of instrument needs artifical manual work to go to accomplish the application of sample process, has complex operation, test result because of the different inconsistent circumstances of operator, is unfavorable for a large amount of popularization.
Therefore, the full-automatic chemiluminescence immunoassay analyzer which is used for single-person reagent strip detection and has the advantages of low cost, small occupied area and convenient operation is very suitable for the market demand.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a full-automatic chemiluminescence immunoassay device, which has small volume and simple and convenient operation and can realize full-automatic detection, aiming at the defects of the prior art.
In recent years, due to the influence of factors such as environment on human body, new onset of infectious diseases has caused a sudden and constant threat to human health, and spread of infectious diseases such as atypical pneumonia, middle east respiratory syndrome, influenza, ebola, zika virus, yellow fever, rift valley fever and the like has become a global public health problem. With the development of urbanization and the increase of global trade, the spread risk of natural epidemic infectious diseases is aggravated by environmental changes, and the prevention and control of infectious diseases are not slow. Therefore, the method for carrying out instant and rapid detection of infectious diseases has great significance for the prevention and control work of infectious diseases.
With the development of society, accurate detection of different diseases and infectious diseases is important. Chemiluminescence is one of the most accurate and mature detection methods of recognized tumor markers and various hormones, the sensitivity and the accuracy of the chemiluminescence are higher than those of an enzyme immunoassay method and a fluorescence method by several orders of magnitude, and the detection result is more authoritative compared with the results obtained by the enzyme immunoassay method and the fluorescence method.
At present, some detection means have some defects, for example, the radioimmunoassay with high detection sensitivity has the problems of radioactive protection and isotope pollution, and moreover, the reagent is expensive and has only one guarantee period, so that the reagent is difficult to popularize in basic medical institutions.
The chemiluminescence immunoassay technology has the advantages of high sensitivity, strong specificity, low reagent price, stable reagent, long effective period (6-18 months), stable and rapid method, wide detection range, simple operation, high automation degree and the like. In recent years, the technique has been rapidly developed as an in vitro diagnostic technique, and is applied to test items of a plurality of disciplines. The device can quickly obtain the detection result, is convenient for patients and reduces the time for the patients to confirm the diagnosis. Meanwhile, the chemiluminescence immunoassay product has the function of continuously using and monitoring the disease condition, for example, a patient with heart disease can quickly know and control the disease condition through detection of interval time, and the chemiluminescence immunoassay can be divided into electrochemical luminescence immunoassay and chemiluminescence immunoassay according to the type and the luminescence characteristics of chemiluminescence substances, wherein the chemiluminescence immunoassay can be divided into direct chemiluminescence immunoassay, enzymatic chemiluminescence immunoassay and luminol oxygen route immunoassay according to different luminescent agents.
The technical scheme adopted by the invention is as follows: the utility model provides a full-automatic chemiluminescence immunoassay device, includes the frame, it deposits module, reagent reaction module, application of sample transportation module, daylighting module and reagent washing separation module to be provided with sample, suction head simultaneously in the frame, and sample, suction head deposit the module setting in reagent reaction module below, and reagent reaction module sets up simultaneously in application of sample transportation module, daylighting module and reagent washing separation module below, and reagent washing separation module sets up between application of sample transportation module and daylighting module. The scheme is based on the principle of magnetic particle enzymatic chemiluminescence immunoassay, adopts paramagnetic particle separation and other modes to realize the separation of free markers and immune compound markers, adopts a PMT single photon counting module to read luminescence signals, has good specificity and high sensitivity, and is a mainstream methodology accepted in clinic. The device can direct detection whole blood or serum sample, and after consumptive material and relevant reagent were prepared to accomplish, the operator only need with the sample place on the sample frame can, through the operation, the device can be full-automatic accomplishes operations such as sample application, final output testing result. The whole device has small volume and simple and convenient operation, and the detection result accuracy and stability of the instrument are not weaker than those of the mainstream large-scale chemiluminescence instrument in the market. Meanwhile, the cost of the analyzer is obviously lower than that of a full-automatic chemiluminescence immunoassay analyzer in the market, and the analyzer is convenient for being popularized in a large amount in the later period.
Furthermore, the sample and sucker storage module comprises a linear guide rail and a first driving mechanism, the linear guide rail and the first driving mechanism are both fixed on the rack, a mounting plate is arranged on the linear guide rail and connected with the first driving mechanism, and the mounting plate can slide along the linear guide rail under the action of the first driving mechanism; the top of the mounting plate is provided with a sample rack, a suction head box and a waste suction head box, and the suction head box is positioned between the sample rack and the waste suction head box; the mounting panel is provided with location sign indicating number tooth one, installs cell type photoelectricity one in the frame, and location sign indicating number tooth one along with the mounting panel removes can with cell type photoelectricity one contact. The main effect is that carrying out depositing of sample and suction head, through a location sign indicating number tooth cooperation cell type photoelectricity accurate positioning suction head and test tube position, can send the test tube of small batch volume and suction head to setting for the position and cooperate application of sample transportation module to accomplish suction head interpolation and sample absorption simultaneously. The sample frame is used for placing test tubes, the suction heads are placed in the suction head boxes, and the waste suction head boxes are used for storing waste suction heads.
The reagent reaction module comprises a reagent reaction support and a driving motor II, the reagent reaction support and the driving motor II are both fixed with the rack, a connecting shaft is arranged on the reagent reaction support, a reagent strip placing plate is sleeved on the connecting shaft and connected with the driving motor II, and the reagent strip placing plate can move along the axis direction of the connecting shaft under the action of the driving motor II; the reagent strip placing plate is provided with a second positioning code tooth, the reagent reaction bracket is provided with a second groove-shaped photoelectric device, and the second positioning code tooth can be contacted with the second groove-shaped photoelectric device along with the movement of the reagent strip placing plate; the reagent strip placing plate is positioned above the sample rack, the suction head box and the waste suction head box. The reagent reaction module is mainly used for placing reagent strips and providing a stable environment for the reaction of reagents and samples, and the driving motor II pulls the reagent strip placing plate and the reagent strips to move together through the synchronous belt II so as to convey the reagent strips to preset positions (such as a sample adding position, a puncture position, a reaction position, a lighting position and the like). The positioning code teeth II are matched with the groove type photoelectric two to realize closed-loop control, so that the motion precision of the whole instrument is improved, and the test precision of the whole instrument is further improved.
Furthermore, the sample adding and transporting module comprises a sample adding support fixed on the rack, a first transverse driving mechanism, a plunger pump and a sample adding device are mounted on the sample adding support, the first transverse driving mechanism and the plunger pump are connected with the sample adding device, the first transverse driving mechanism can drive the sample adding device to move transversely, and the sample adding device can move vertically relative to the sample adding support; the sample adding device comprises a first vertical driving mechanism and a first vertical mounting plate, the first vertical driving mechanism is mounted on the first vertical mounting plate, a horizontal guide rail is arranged on the side wall of the sample adding support, and the first vertical mounting plate is connected with the horizontal guide rail and can move along the horizontal guide rail under the action of a first transverse driving mechanism; the vertical guide rail is arranged on the vertical mounting plate, the mounting block is arranged on the vertical guide rail and connected with the first vertical driving mechanism, the mounting block can move along the vertical guide rail under the action of the first vertical driving mechanism, the mounting block is connected with the gun head, and the gun head is connected with the plunger pump. The sample adding and transporting module is mainly used for connecting the injection plug pump with the gun head and the suction head, and functions of adding, transporting and injecting the suction head into a sample are realized. Simultaneously, the functions of gun head detection and liquid level detection are also realized.
Further, the reagent cleaning and separating module comprises a reagent cleaning and separating support frame, the reagent cleaning and separating support frame is installed on the rack, a magnetic rod stirring and mixing device and a puncturing device are installed on the reagent cleaning and separating support frame, and the magnetic rod stirring and mixing device and the puncturing device can vertically move relative to the reagent cleaning and separating support frame; the puncture device comprises a puncture driving mechanism and a magnetic rod sleeve mounting support, the puncture driving mechanism is mounted on the reagent cleaning and separating support, the magnetic rod sleeve mounting support is connected with the puncture driving mechanism and can vertically move relative to the reagent cleaning and separating support under the action of the puncture driving mechanism, and a plurality of magnetic rod sleeves and puncture membrane heads are mounted on the magnetic rod sleeve mounting support; the magnetic bar stirring and mixing device comprises a magnetic bar driving mechanism and a magnetic bar support, the magnetic bar support is connected with a magnetic bar sleeve mounting support, the magnetic bar driving mechanism is mounted on the magnetic bar support, a magnetic bar mounting block is arranged below the magnetic bar support and connected with the magnetic bar driving mechanism, the magnetic bar mounting block can vertically move relative to the reagent cleaning and separating support frame under the action of the magnetic bar driving mechanism, a plurality of magnetic bars are mounted on the magnetic bar mounting block, and the magnetic bars can penetrate through the magnetic bar sleeve mounting support and are inserted into corresponding magnetic bar sleeves; be provided with a plurality of guiding axles on the bar magnet installation piece, the guiding axle all passes the bar magnet installation piece, and both ends are connected with bar magnet support and bar magnet cover installing support respectively, and the bar magnet installation piece can remove along the axis of guiding axle. The reagent cleaning and separating module is mainly used for completing the reaction of a sample and a reagent, extracting paramagnetic beads in the reaction and completing cleaning and transferring.
The lighting module comprises a lighting transverse driving mechanism, a lighting vertical driving mechanism and a PMT support, the lighting transverse driving mechanism is installed on the reagent cleaning and separating support frame, the lighting vertical driving mechanism is installed on the PMT support, a lighting horizontal guide rail and a lighting synchronous belt which are parallel to each other are arranged on the reagent cleaning and separating support frame, the lighting synchronous belt is connected with the lighting transverse driving mechanism, the PMT support is simultaneously connected with the lighting horizontal guide rail and the lighting synchronous belt and can horizontally move along the lighting horizontal guide rail, the lighting vertical driving mechanism is connected with a PMT mounting support, the PMT mounting support can vertically move under the action of the lighting vertical driving mechanism, and the PMT mounting support is provided with a PMT module; the PMT support is provided with a lighting longitudinal guide rail, and the PMT mounting support is connected with the lighting longitudinal guide rail and can vertically move along the lighting longitudinal guide rail. The lighting module is mainly used for collecting and amplifying the luminous reactant, converting the signal into a motor signal and transmitting the motor signal to the data processing and control module.
The invention has the beneficial effects that:
1. the scheme can realize automatic addition and removal of the suction head, the magnetic rod sleeve and the membrane breaking head;
2. the scheme can realize automatic sample adding and sample arrangement;
3. the conductive suction head is matched with a liquid level detection system, and the depth of the suction head inserted below the liquid level of a sample is accurately controlled, so that the sample adding precision requirement of the whole device is guaranteed;
4. the reagent strip adopts a sealing and packaging mode, so that the quality guarantee period of the reagent is relatively long, and the reagent is convenient to transport;
5. the scheme can monitor the state of the suction head in real time, and can feed back the abnormal state (such as not adding successfully, dropping in midway for removing successfully and running, and the like) of the suction head in time and correct correspondingly when necessary, thereby enabling the whole device to be more intelligent.
Drawings
FIG. 1 is a schematic flow diagram of the present invention;
FIG. 2 is a schematic structural view of the present invention;
FIG. 3 is a schematic diagram of a sample and tip storage module;
FIG. 4 is a schematic structural view of a reagent reaction module;
FIG. 5 is a schematic structural view of a sample application transport module;
FIG. 6 is a schematic structural view of a lighting module;
FIG. 7 is a schematic diagram of the structure of the reagent cleaning and separating module.
In the figure: 1-a sample, suction head storage module; 2-a reagent reaction module; 3-sample adding and transporting module; 4-a lighting module; 5-a reagent cleaning and separating module; 6-linear guide rail; 7-a sample rack; 8-a suction head box; 9-waste pipette tip cartridge; 10-groove type photoelectricity one; 11-positioning code teeth I; 12-a first drive mechanism; 13-synchronous belt I; 14-a synchronous pulley I; 15-reagent reaction holder; 16-reagent strip placing plate; 17-a linear bearing; 18-positioning code teeth II; 19-synchronous belt two; 20-driving a second motor; 21-groove type photoelectric element II; 22-pulley mounting bracket; 23-synchronous pulley two; 24-column one; 25-a suction head; 26-gun head; 27-a transverse mounting plate; 28-synchronous pulley III; 29-plunger pump; 30-a first vertical driving mechanism; 31-a transverse driving mechanism I; 32-synchronous belt III; 33-horizontal guide rails; 34-a vertical mounting plate; 35-column two; 36-PMT module; 37-daylighting horizontal guide rail; 38-lighting synchronous belt wheel; 39-lighting vertical driving mechanism; 40-a lighting transverse driving mechanism; 41-lighting synchronous belt; 42-lighting mounting plate; 43-a magnet rod sleeve; 44-a magnetic bar mounting block; 45-bar magnet holder; 46-a magnetic bar drive mechanism; 47-a lancing drive mechanism; 48-a guide shaft; 49-magnetic bar sleeve mounting bracket; 50-a magnetic bar; 51-a mounting plate; 52-a connecting shaft; 53-PMT support; 54-PMT mounting bracket.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the description of the present application, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a particular orientation, be constructed in a particular orientation, and be operated, and thus should not be construed as limiting the present application.
The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless otherwise specified.
In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
Example (b):
as shown in fig. 1 and fig. 2, a full-automatic chemiluminescence immunoassay device comprises a frame, wherein a sample and suction head storage module 1, a reagent reaction module 2, a sample adding transportation module 3, a lighting module 4 and a reagent cleaning and separating module 5 are arranged on the frame, the sample and suction head storage module 1 is arranged below the reagent reaction module 2, the reagent reaction module 2 is arranged below the sample adding transportation module 3, the lighting module 4 and the reagent cleaning and separating module 5, and the reagent cleaning and separating module 5 is arranged between the sample adding transportation module 3 and the lighting module 4. The basic operation of the test bench comprises the steps of putting a sample and a corresponding single-person reagent strip, consumable materials and the like, and automatically executing the sample test through the operation. The process comprises the steps of sucking a sample, and then adding the sample into a corresponding reagent strip by using a sucker through a full-automatic mechanical arm for reaction. The sample is fully reacted in the reagent strip and washed for several times, then the luminous value of the sample is detected, the data is processed, and the detection result is output, so that the full-automatic single-person sample detection is finally realized.
As shown in fig. 3, the sample and pipette tip storage module 1 includes a linear guide 6 and a first driving mechanism 12, the linear guide 6 and the first driving mechanism 12 are both fixed on the rack, a mounting plate 51 is disposed on the linear guide 6, the mounting plate 51 is connected with the first driving mechanism 12, and the mounting plate 51 can slide along the linear guide 6 under the action of the first driving mechanism 12; the top of the mounting plate 51 is provided with a sample rack 7, a suction head box 8 and a waste suction head box 9, and the suction head box 8 is positioned between the sample rack 7 and the waste suction head box 9; the mounting plate 51 is provided with a first positioning code tooth 11, a first groove-shaped photoelectric device 10 is mounted on the rack, and the first positioning code tooth 11 can be in contact with the first groove-shaped photoelectric device 10 along with the movement of the mounting plate 51. The first synchronous belt wheels 14 are two, one is connected with an output shaft of the first driving mechanism 12, the other can be connected with the rack, the first synchronous belt wheels 14 are sleeved with the first synchronous belt 13, the first synchronous belt 13 is connected with the mounting plate 51, the first driving mechanism 12 drives the first synchronous belt 13 to move to provide power for the mounting plate 51 to slide along the linear guide rail 6, and the linear guide rail 6 is used for limiting the moving direction of the mounting plate 51. Through a 11 cooperation cell type photoelectricity of location sign indicating number tooth a 10 accurate positioning suction head and test tube position, can send the test tube of small batch and suction head to setting for the position and cooperate application of sample transportation module to accomplish suction head interpolation and sample absorption simultaneously.
As shown in fig. 4, the reagent reaction module comprises a reagent reaction support 15 and a second driving motor 20, both the reagent reaction support 15 and the second driving motor 20 are fixed with the rack, a connecting shaft 52 is arranged on the reagent reaction support 15, a reagent strip placing plate 16 is sleeved on the connecting shaft 52, the reagent strip placing plate 16 is connected with the second driving motor 20, and the reagent strip placing plate 16 can move along the axial direction of the connecting shaft 52 under the action of the second driving motor 20; the reagent strip placing plate 16 is provided with a second positioning code tooth 18, the reagent reaction bracket 15 is provided with a second groove-shaped photoelectric bracket 21, and the second positioning code tooth 18 can be contacted with the second groove-shaped photoelectric bracket 21 along with the movement of the reagent strip placing plate 16; the reagent strip placement plate 16 is located above the sample rack 7, the tip cassette 8, and the waste tip cassette 9. Reagent reaction support 15 adopts column type bearing structure, connecting axle 52 is the optical axis, connecting axle 52 is preferred two, it is located inside reagent reaction support 15, both ends are fixed with reagent reaction support 15 respectively, all overlap on the connecting axle 52 and have linear bearing 17, linear bearing 17 can remove along the axis of connecting axle 52, the board 16 is placed to the reagent strip is fixed with two linear bearing 17 outer walls simultaneously, can remove along with linear bearing 17 together, this kind of structure makes the removal steady. Because the cavity below the reagent reaction support 15 needs to reserve the sample rack 7, the suction head box 8 and the waste suction head box 9, the reagent reaction support 15 is provided with the belt wheel mounting support 22, a part of the second synchronous belt wheels 23 are mounted on the reagent reaction support 15, the second synchronous belt wheels 23 are mounted on the output shaft of the second driving motor 20, the second synchronous belt 19 is connected with the second synchronous belt wheels 23 at the same time and fixed with the outer wall of the linear bearing 17 through the connecting sheet, power is provided for the movement of the reagent strip placing plate 16, the positioning code teeth 18 are matched with the groove type photoelectric wheels 21 to realize closed-loop control, the movement precision of the whole instrument is improved, and the test precision of the whole instrument is further improved.
As shown in fig. 5, the sample-adding transportation module includes a sample-adding support fixed on the rack, a first transverse driving mechanism 31, a plunger pump 29 and a sample-adding device are mounted on the sample-adding support, the first transverse driving mechanism 31 and the plunger pump 29 are both connected with the sample-adding device, the first transverse driving mechanism 31 can drive the sample-adding device to move transversely, and the sample-adding device can move vertically relative to the sample-adding support; the sample adding device comprises a first vertical driving mechanism 30 and a vertical mounting plate 34, the first vertical driving mechanism 30 is mounted on the vertical mounting plate 34, a horizontal guide rail 33 is arranged on the side wall of the sample adding support, and the vertical mounting plate 34 is connected with the horizontal guide rail 33 and can move along the horizontal guide rail 33 under the action of a first transverse driving mechanism 31; the vertical mounting plate 34 is provided with a vertical guide rail, the vertical guide rail is provided with a mounting block, the mounting block is connected with a first vertical driving mechanism 30, the mounting block can move along the vertical guide rail under the action of the first vertical driving mechanism 30, the mounting block is connected with a gun head 26, and the gun head 26 is connected with a plunger pump 29. The sample adding support is composed of first upright posts 24 and a transverse mounting plate 27, wherein the transverse mounting plate 27 is located between the first upright posts 24 and fixed with the tops of the first upright posts 24, a first transverse driving mechanism 31 and a plunger pump 29 are fixed on the tops of the transverse mounting plate 27, a horizontal guide rail 33 and a third synchronous pulley 28 are mounted on the side wall of the transverse mounting plate 27, the third synchronous pulley 32 is connected with all the third synchronous pulleys 28 at the same time, the first transverse driving mechanism 31 drives the third synchronous pulley 32 to move to provide power for the vertical mounting plate 34 to move along the horizontal guide rail 33, the gun head 26 is connected with a suction head 25, the plunger pump 29 is connected with the gun head 26 and the suction head 25, and the functions of adding, transferring and injecting samples by the suction head are achieved. Simultaneously, the functions of gun head detection, liquid level detection and bar code scanning are also accompanied.
As shown in fig. 7, the reagent cleaning and separating module 5 includes a reagent cleaning and separating support frame, the reagent cleaning and separating support frame is installed on the rack, a magnetic rod stirring and mixing device and a puncturing device are installed on the reagent cleaning and separating support frame, and both the magnetic rod stirring and mixing device and the puncturing device can vertically move relative to the reagent cleaning and separating support frame; the puncturing device comprises a puncturing driving mechanism 47 and a magnetic rod sleeve mounting bracket 49, the puncturing driving mechanism 47 is mounted on the reagent cleaning and separating support frame, the magnetic rod sleeve mounting bracket 49 is connected with the puncturing driving mechanism 47, the magnetic rod sleeve mounting bracket 49 can vertically move relative to the reagent cleaning and separating support frame under the action of the puncturing driving mechanism 47, and a plurality of magnetic rod sleeves 43 and puncturing film heads are mounted on the magnetic rod sleeve mounting bracket 49; the magnetic rod stirring and blending device comprises a magnetic rod driving mechanism 46 and a magnetic rod support 45, the magnetic rod support 45 is connected with a magnetic rod sleeve mounting support 49, the magnetic rod driving mechanism 46 is mounted on the magnetic rod support 45, a magnetic rod mounting block 44 is arranged below the magnetic rod support 45, the magnetic rod mounting block 44 is connected with the magnetic rod driving mechanism 46, the magnetic rod mounting block 44 can vertically move relative to the reagent cleaning and separating support frame under the action of the magnetic rod driving mechanism 46, a plurality of magnetic rods 50 are mounted on the magnetic rod mounting block 44, and the magnetic rods 50 can penetrate through the magnetic rod sleeve mounting support 49 and be inserted into corresponding magnetic rod sleeves 43; the magnetic bar mounting block 44 is provided with a plurality of guide shafts 48, the guide shafts 48 penetrate through the magnetic bar mounting block 44, two ends of each guide shaft are respectively connected with the magnetic bar bracket 45 and the magnetic bar sleeve mounting bracket 49, the magnetic bar mounting block 44 can move along the axis of the guide shaft 48, the guide shafts 48 are used for moving and guiding the magnetic bar mounting block 44, the number of the guide shafts 48 is generally two, and a screw rod connected with the magnetic bar driving mechanism 46 is positioned between the guide shafts 48. The reagent cleaning and separating support frame is composed of two upright posts 35 and a lighting mounting plate 42, the top surfaces of the two upright posts 35 are vertically fixed with the lighting mounting plate 42, the reagent cleaning and separating module 5 and the lighting module 4 share one support frame, and the puncture driving mechanism 47 is installed on the top surface of the lighting mounting plate 42. The lead screw of the puncturing drive mechanism 47 penetrates through the lighting installation plate 42 and then is connected with the magnetic rod sleeve installation support 49, and the puncturing drive mechanism 47 rotates to drive the magnetic rod sleeve installation support 49 to move in the vertical direction. The reagent cleaning and separating module is mainly used for completing the reaction of a sample and a reagent, extracting paramagnetic beads in the reaction and completing cleaning and transferring. For the convenience of assembly, the bar magnet housing mounting bracket 49 may be designed to have an L-shaped section.
As shown in fig. 6, the lighting module 4 includes a lighting transverse driving mechanism 40, a lighting vertical driving mechanism 39 and a PMT bracket 53, the lighting transverse driving mechanism 40 is installed on the top surface of the lighting installation plate 42, the lighting vertical driving mechanism 39 is installed on the PMT bracket 53, a reagent cleaning and separating support frame is provided with a lighting horizontal guide rail 37 and a lighting synchronous belt 41 which are parallel to each other, the lighting synchronous belt 41 is connected with the lighting transverse driving mechanism 40, the PMT bracket 53 is simultaneously connected with the lighting horizontal guide rail 37 and the lighting synchronous belt 41 and can horizontally move along the lighting horizontal guide rail 37, the lighting vertical driving mechanism 39 is connected with a PMT installation bracket 54, the PMT installation bracket 54 can vertically move under the action of the vertical driving mechanism 39, and the PMT installation bracket 54 is provided with a PMT module 36; the PMT support 53 is provided with a lighting longitudinal guide rail, the PMT mounting support 54 is connected with the lighting longitudinal guide rail and can vertically move along the lighting longitudinal guide rail (the lighting longitudinal guide rail is blocked and not shown in figure 6), and the lighting module is mainly used for collecting and amplifying a luminous reactant, converting a signal into a motor number and transmitting the motor number to the data processing and control module.
The scheme is matched with the integrated reagent card and the disposable sucker, and can realize the functions of automatically grabbing and installing the disposable sucker and the magnetic rod sleeve; the single test has large sample amount, and can automatically sort and identify samples and record information into the system; automatic sample adding; multiple channels can be detected in parallel; automatically puncturing the reagent; providing a constant-temperature immune reaction environment, and carrying out real-time monitoring, feedback and adjustment; automatically cleaning immunoreaction calibration substances for different times according to different requirements, and transferring; PMT automatic lighting and signal transmission and analysis, and can carry out multiple detection and comparison; after detection, automatically removing the suction head and the magnetic rod sleeve; and the sample liquid level monitoring can be realized by matching the conductive suction head, so that the high-precision sample suction is realized.
After the device is started, the reagent reaction module 2 can be preheated. After the samples and corresponding single-person reagent strips, consumables and the like are manually put in, the sample storage module automatically sorts and positions the samples, and the bar codes scan, identify and record sample information. The reagent reaction module 2 heats the reagent card strip and carries out real-time feedback regulation and control. The reagent cleaning and separating module 5 automatically captures the disposable membrane pricking head, and automatically punctures the sealing membrane of the corresponding test item of the reagent card strip and then removes the membrane pricking head. The sample adding transportation module 3 automatically grabs the disposable suction head and absorbs a sample, and then transfers the sample solution to the upper part of the corresponding reagent card strip, and the sample adding assembly drops the sample solution to the corresponding reaction area of the reagent strip and automatically removes the disposable suction head. The sample and the reagent are sufficiently reacted in the reagent reaction module. The reagent cleaning and separating module 5 grabs a magnetic rod protective sleeve at the front end of the reagent strip, moves to a reagent strip sample reaction area after reaction is completed, adsorbs paramagnetic particles combined with the sample to the magnetic rod protective sleeve through the magnetic rod, and performs cleaning for different times according to different items to remove impurities. And after the reagent cleaning and separating module runs to a lighting area, the magnetic rod is extracted, and the paramagnetic particles fall off. The detection module is used for lighting in a lighting area, converting an optical signal into an electric signal, and performing data analysis and outputting a result through the control system. The reagent cleaning and separating module automatically removes the magnetic bar protective sleeve.
The whole scheme realizes automatic addition and removal of the suction head, the magnetic rod sleeve and the membrane breaking head; automatic sample adding and sample discharging are realized; the conductive suction head is matched with a liquid level detection system, and the depth of the suction head inserted below the liquid level of a sample is accurately controlled, so that the sample adding precision requirement of the whole device is guaranteed; the reagent strip adopts a sealing and packaging mode, so that the quality guarantee period of the reagent is relatively long, and the reagent is convenient to transport; the state of the suction head is monitored in real time, and when the suction head is in an abnormal state (such as unsuccessfully adding, successfully removing, falling in the midway of operation and the like), the suction head is timely fed back and correspondingly corrected if necessary, so that the whole device is more intelligent.
The above-mentioned embodiments only express the specific embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (10)
1. The utility model provides a full-automatic chemiluminescence immunoassay device, includes the frame, a serial communication port, be provided with the sample simultaneously in the frame, the suction head deposits module (1), reagent reaction module (2), application of sample transportation module (3), daylighting module (4) and reagent washing separation module (5), and the sample, suction head deposit module (1) and set up in reagent reaction module (2) below, reagent reaction module (2) set up simultaneously in application of sample transportation module (3), daylighting module (4) and reagent washing separation module (5) below, reagent washing separation module (5) set up between application of sample transportation module (3) and daylighting module (4).
2. The full-automatic chemiluminescence immunoassay device according to claim 1, wherein the sample and pipette tip storage module (1) comprises a linear guide rail (6) and a first driving mechanism (12), the linear guide rail (6) and the first driving mechanism (12) are both fixed on the rack, a mounting plate (51) is arranged on the linear guide rail (6), the mounting plate (51) is connected with the first driving mechanism (12), and the mounting plate (51) can slide along the linear guide rail (6) under the action of the first driving mechanism (12); the top of the mounting plate (51) is provided with a sample rack (7), a suction head box (8) and a waste suction head box (9), and the suction head box (8) is positioned between the sample rack (7) and the waste suction head box (9); the mounting plate (51) is provided with a first positioning code tooth (11), a first groove-shaped photoelectric device (10) is mounted on the rack, and the first positioning code tooth (11) can be in contact with the first groove-shaped photoelectric device (10) along with the movement of the mounting plate (51).
3. The full-automatic chemiluminescence immunoassay device according to claim 2, wherein the reagent reaction module (2) comprises a reagent reaction bracket (15) and a second driving motor (20), both the reagent reaction bracket (15) and the second driving motor (20) are fixed with the rack, a connecting shaft (52) is arranged on the reagent reaction bracket (15), a reagent strip placing plate (16) is sleeved on the connecting shaft (52), the reagent strip placing plate (16) is connected with the second driving motor (20), and the reagent strip placing plate (16) can move along the axial direction of the connecting shaft (52) under the action of the second driving motor (20); the reagent strip placing plate (16) is provided with a second positioning code tooth (18), the reagent reaction bracket (15) is provided with a second groove-shaped photoelectric tooth (21), and the second positioning code tooth (18) can be contacted with the second groove-shaped photoelectric tooth (21) along with the movement of the reagent strip placing plate (16); the reagent strip placing plate (16) is positioned above the sample rack (7), the suction head box (8) and the waste suction head box (9).
4. The full-automatic chemiluminescence immunoassay device according to claim 1, wherein the sample feeding transportation module (3) comprises a sample feeding support fixed on the rack, a first lateral driving mechanism (31), a plunger pump (29) and a sample feeding device are mounted on the sample feeding support, the first lateral driving mechanism (31) and the plunger pump (29) are both connected with the sample feeding device, the first lateral driving mechanism (31) can drive the sample feeding device to move laterally, and the sample feeding device can move vertically relative to the sample feeding support.
5. The full-automatic chemiluminescence immunoassay device according to claim 4, wherein the sample adding device comprises a first vertical driving mechanism (30) and a vertical mounting plate (34), the first vertical driving mechanism (30) is mounted on the vertical mounting plate (34), a horizontal guide rail (33) is arranged on the side wall of the sample adding bracket, and the vertical mounting plate (34) is connected with the horizontal guide rail (33) and can move along the horizontal guide rail (33) under the action of a first transverse driving mechanism (31); the vertical driving mechanism is characterized in that a vertical guide rail is arranged on the vertical mounting plate (34), a mounting block is arranged on the vertical guide rail and is connected with a first vertical driving mechanism (30), the mounting block can move along the vertical guide rail under the action of the first vertical driving mechanism (30), the mounting block is connected with a gun head (26), and the gun head (26) is connected with a plunger pump (29).
6. The full-automatic chemiluminescence immunoassay device according to any one of claims 1 to 5, wherein the reagent cleaning and separating module (5) comprises a reagent cleaning and separating support frame, the reagent cleaning and separating support frame is mounted on a rack, a magnetic rod stirring and mixing device and a puncturing device are mounted on the reagent cleaning and separating support frame, and both the magnetic rod stirring and mixing device and the puncturing device can vertically move relative to the reagent cleaning and separating support frame.
7. The full-automatic chemiluminescence immunoassay device according to claim 6, wherein the puncturing device comprises a puncturing drive mechanism (47) and a magnetic rod sleeve mounting bracket (49), the puncturing drive mechanism (47) is mounted on the reagent cleaning and separating support frame, the magnetic rod sleeve mounting bracket (49) is connected with the puncturing drive mechanism (47), the magnetic rod sleeve mounting bracket (49) can vertically move relative to the reagent cleaning and separating support frame under the action of the puncturing drive mechanism (47), and a plurality of magnetic rod sleeves (43) and puncturing film heads are mounted on the magnetic rod sleeve mounting bracket (49).
8. The full-automatic chemiluminescence immunoassay device according to claim 7, wherein the magnetic rod stirring and mixing device comprises a magnetic rod driving mechanism (46) and a magnetic rod support (45), the magnetic rod support (45) is connected with a magnetic rod sleeve mounting support (49), the magnetic rod driving mechanism (46) is mounted on the magnetic rod support (45), a magnetic rod mounting block (44) is arranged below the magnetic rod support (45), the magnetic rod mounting block (44) is connected with the magnetic rod driving mechanism (46), the magnetic rod mounting block (44) can vertically move relative to the reagent cleaning and separating support under the action of the magnetic rod driving mechanism (46), a plurality of magnetic rods (50) are mounted on the magnetic rod mounting block (44), and the magnetic rods (50) can penetrate through the magnetic rod sleeve mounting support (49) and be inserted into corresponding magnetic rod sleeves (43).
9. The full-automatic chemiluminescence immunoassay device according to claim 8, wherein a plurality of guide shafts (48) are arranged on the magnetic rod mounting block (44), the guide shafts (48) pass through the magnetic rod mounting block (44), and both ends of the guide shafts are respectively connected with the magnetic rod bracket (45) and the magnetic rod sleeve mounting bracket (49), and the magnetic rod mounting block (44) can move along the axis of the guide shafts (48).
10. The full-automatic chemiluminescence immunoassay device according to any one of claims 6 to 9, wherein the lighting module (4) comprises a lighting transverse driving mechanism (40), a lighting vertical driving mechanism (39) and a PMT bracket (53), the lighting transverse driving mechanism (40) is installed on the reagent cleaning separation support frame, the lighting vertical driving mechanism (39) is installed on the PMT bracket (53), the reagent cleaning separation support frame is provided with a lighting horizontal guide rail (37) and a lighting synchronous belt (41) which are parallel to each other, the lighting synchronous belt (41) is connected with the lighting transverse driving mechanism (40), the PMT bracket (53) is simultaneously connected with the lighting horizontal guide rail (37) and the lighting synchronous belt (41) and can horizontally move along the lighting horizontal guide rail (37), the lighting vertical driving mechanism (39) is connected with a PMT mounting bracket (54), the PMT mounting bracket (54) can vertically move under the action of the lighting vertical driving mechanism (39), and a PMT module (36) is arranged on the PMT mounting bracket (54); and the PMT support (53) is provided with a lighting longitudinal guide rail, and the PMT mounting support (54) is connected with the lighting longitudinal guide rail and can vertically move along the lighting longitudinal guide rail.
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| CN113820195A (en) * | 2021-11-25 | 2021-12-21 | 成都宜乐芯生物科技有限公司 | Multichannel parallel pretreatment device |
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| CN113567696B (en) | 2024-07-02 |
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