CN106018784B - Small electrical chemical illumination immunity analysis instrument and its analysis method - Google Patents
Small electrical chemical illumination immunity analysis instrument and its analysis method Download PDFInfo
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- CN106018784B CN106018784B CN201610521811.5A CN201610521811A CN106018784B CN 106018784 B CN106018784 B CN 106018784B CN 201610521811 A CN201610521811 A CN 201610521811A CN 106018784 B CN106018784 B CN 106018784B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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Abstract
The present invention discloses a kind of small electrical chemical illumination immunity analysis instrument, the rack of platform is equipped with including bottom, platform is equipped with special agent unit, sample storehouse unit, hatch unit, common reagent unit, the back upper place of rack is equipped with upper mounting plate, upper mounting plate is equipped with mechanical arm unit, the machinery arm unit includes the first mechanical arm equipped with sample needle and the second mechanical arm equipped with post-processing needle, it is additionally provided on the rack for measuring the measurement analytical unit for analyzing incubation reaction sample, for controlling sample, the fluid path unit of reagent and waste liquid flow direction, display unit for human-computer interaction, for carrying out the board control unit of circuit control to each unit.The medicine have the advantages that the analyzer is compact-sized, occupied area is small, reagent can be with long-term preservation, it is entire to be incubated for, measure analytic process high degree of automation, human assistance is not needed, human error is reduced, there is excellent measurement accuracy, it measures easy to operate, greatly improves working efficiency.
Description
Technical field
The present invention relates to a kind of biological body fluid or blood sample analysis device, in particular to a kind of small electrical chemiluminescence
Immunity analysis instrument and its analysis method.
Background technique
Electrogenerated chemiluminescent immunoassay instrument is mainly used for clinical blood, analyte assay in other body fluid samples.Change
Learning luminescence immunoassay (CLIA) is the technology worldwide to grow up in the past 20 years, relative to radiommunoassay
(RIA), the immuno analytical methods such as EIA enzyme immunoassay (EIA), fluoroimmunoassay (FIA) have environmental protection, not by temperature and soda acid
The advantages that spending influence, reagent long shelf-life, accuracy and high sensitivity.Therefore chemiluminescence immunoassay is widely used in body
Outer diagnosis field (IVD).
Chemiluminescence immunoassay type is more, such as the direct chemoluminescence method based on acridinium ester, is based on alkaline phosphatase
The indirect chemiluminescence method of enzyme, Electrochemiluminescince etc..The luminescence process of direct chemoluminescence method and enzyme touching luminescent method is by instrument
Device addition exciting liquid starts, therefore fluorescent lifetime is related to liquid addition manner.Electrochemical luminescence process fluorescent lifetime is by electrode
Power-up time control, therefore fluorescent lifetime consistency is good.Electrochemical luminescence apparatus measures analytical unit generallys use pipeline sample introduction
Design, the relatively direct open input mode of chemiluminescence, can effectively avoid open mould group light leakage and photomultiplier tube is sudden and violent
The risk of dew under ambient light.
There are Roche cobas E411 and E601 using the common instrument of Electrogenerated chemiluminescent immunoassay method, though these instruments
Right test speed is fast, the degree of automation is higher, but occupies larger space, and the occasion for being unfavorable for less test volume uses, and this
A little instrument category import equipments, expensive and reagent test is at high cost, therefore in small hospital, the small department of large hospital and She Kang
Center is unable to get universal use.Existing small chemical luminescence immunoassay instrument generallys use semi-automatic design, eliminates incubation
Cup feeding processing unit, incubation reaction device, Magneto separate cleaning device etc., only remain luminous measuring device and control system.
This quasi-instrument saves instrument cost, reduces volume, but sample-adding, reagent adding, incubation reaction process are required to manual operation,
When increasing the complexity of test, and carrying out multiple test samples at the same time, it is be easy to cause operation error, and then affects test
As a result accuracy, there are security risks.Another existing fully automatic electric chemiluminescent analyzer, structure is complicated, and volume is larger；It is existing
Fully automatic electric chemiluminescent analyzer, carry out low-temperature preservation typically no to reagent cannot place and be detected the long period；
Existing fully automatic electric chemiluminescent analyzer, due to needing integrally to deliver reaction cup to its measuring device, unavoidable need
Measuring device is designed as to open mould group, photon extraneous in this way easily enters in measuring device, while each reaction
Cup enter measuring device when form it is different, cause measurement when, for reading photomultiplier tube PMT obtain faint number of photons because
It is easy to be influenced by the entrance of extraneous photon, leads to very large deviation occur, cause the measurement accuracy of entire measuring system not high；Again
Existing fully automatic electric chemiluminescent analyzer, typically no touch display unit, no man-machine interactive operating system, operation
On it is not convenient enough.
Summary of the invention
It is an object of the invention to provide a kind of small electrical chemiluminescence immune assay to overcome the shortcomings of above-mentioned technology
Instrument, which has all automatic measurement analytic function, small in size, easy to use, and measurement is efficiently and measurement accuracy is high.
The technical proposal of the invention is realized in this way, and the small electrical chemical illumination immunity analysis instrument, including bottom are equipped with
The rack of platform, the platform are equipped with special agent unit, sample storehouse unit, hatch unit, common reagent unit；It is described special
It include dedicated kit, dedicated kit mounting groove, the mixing mechanism for stirring special agent and for low with reagent unit
First temperature control refrigerating plant of warm preservation dedicated kit；The hatch unit includes the incubation rotated by incubation motor driven
Disk is incubated for the incubation cup groups for integrating multiple incubation cups put in disk and is incubated for the temperature control heating diaphragm that disk lower part is equipped with；
The common reagent unit includes the second temperature control refrigeration dress that universal kit, universal kit mounting groove and its underpart are equipped with
It sets；The rack back upper place is equipped with upper mounting plate, and the upper mounting plate is equipped with mechanical arm unit, and the machinery arm unit includes being equipped with
The first mechanical arm of sample needle and equipped with post-processing needle second mechanical arm, the sample needle the first mechanical arm drive under
It can be moved freely above the special agent unit, sample storehouse unit, hatch unit, the post-processing needle is in second machine
It can be moved freely above the hatch unit, common reagent unit under tool armband is dynamic；Measurement analysis is additionally provided in the rack
Unit, fluid path unit, the display unit for carrying out human-computer interaction for controlling sample and reagent flow direction and collection waste liquid
And central controlled board control unit is used for remaining described each unit circuit connection.
Preferably, the first temperature control refrigerating plant or the second temperature control refrigerating plant, including bottom plate that can be thermally conductive, the bottom
It includes cold that plate is equipped with refrigeration conductive plate, semiconductor refrigeration chip, cooling fin and fan, the semiconductor refrigeration chip in turn below
Hot two sides, the hot face are fitted and connected on one side with the cooling fin, and the fan is connect to dissipate with the cooling fin another side
Heat, the huyashi-chuuka (cold chinese-style noodles) are fitted and connected on one side with the refrigeration conductive plate, the refrigeration conductive plate another side and the bottom plate lower plane
It is fitted and connected, plane is equipped with the dedicated kit mounting groove or universal kit mounting groove on the bottom plate.
Preferably, the dedicated kit include box body, magnetic bead bottle, the reagent bottle put in box body, the magnetic bead bottle, examination
Special agent can be filled in agent bottle respectively, the magnetic bead bottle is freely rotatable, and its underpart is equipped with driving section and is exposed to the box body；
The mixing mechanism includes mixing motor, mixing driving member, and the mixing driving member is connect with the driving section of the magnetic bead bottle, institute
Stating mixing motor can drive the mixing driving member that the magnetic bead bottle is driven to carry out reciprocating rotation.
Preferably, the sample storehouse unit includes for putting the sample rack of sample tube, sample rack mounting base, sample rack
Positioning device and sample service sink component, the sample service sink component include sample service sink, are connected with the fluid path unit
Detergent line, waste liquid discharge line.
Preferably, the incubation disk corresponds with the incubation cup and can accommodate described incubate in box-like, internal be equipped with of circle
Educate the incubation hole of cup, can be placed four groups in the incubation disk described in incubation cup group, the incubation cup group includes a quarter annulus
The cup holder of shape and vertical sleeve are in several incubation cups on cup holder.
Preferably, the cup that is incubated for is equipped with the cup inner cavity that can contain reaction solution, and the incubation cup includes upper cup body and lower cup
Body, the upper cup body in a tubular form, receive after the upper cup body in the inverted conical shape being gradually reduced by the inside and outside wall of the lower cup body
And bottom transition be circular shape, the cup holder be equipped with glass holder hole, the upper cup body unremovably sleeve in the cup
In sets of holes, the lower cup body is exposed to the cup holder.
Preferably, the incubation disk top be equipped be incubated for disk cover, the incubations disk cover be equipped with adding mouth, for loading and
Take out the entrance for being incubated for cup group.
Preferably, the hatch unit further includes the code-disc with the incubation disk coaxial arrangement, and the code-disc lower part is equipped with
Code-disc zero-bit inductor and code-disc rotation precision inductor.
Preferably, the hatch unit further includes being incubated for cleaning assembly and wash bowl, and the incubation cleaning assembly includes incubating
Service sink, the detergent line being connected with the fluid path unit, waste liquid discharge line are educated, the wash bowl is clear by force for storage system
Washing lotion reagent bottle.
Preferably, the first mechanical arm is three-dimensional arm, and the second mechanical arm is two-dimentional arm, the three-dimensional arm and two dimension
Arm is mounted on the same root X-direction guide rail that the upper mounting plate is equipped with, and the three-dimensional arm includes can be free on X-direction guide rail
Mobile three-dimensional Y-axis arm, the three-dimensional Z axis arm that can move freely along Y-direction installed on the three-dimensional Y-axis arm, the sample-adding
Needle is parallelly mounted on the three-dimensional Z axis arm and can move freely along Z-direction；The two dimension arm includes can on X-direction guide rail
Free-moving two dimension Y-axis arm, the two-dimentional Z axis arm being fixedly mounted on the two-dimentional Y-axis arm, the post-processing needle are installed in parallel
In can move freely on the two-dimentional Z axis arm and along Z-direction；The fortune of the three-dimensional arm and two-dimentional arm on each movable direction
It is dynamic to be driven respectively by respective motor and synchronous belt.
Preferably, the measurement analytical unit includes for measuring the measurement component for being incubated for sample, outsourcing arrangement, using
In the temperature-controlling component, the analysis processing circuit that control the measurement component temperature, the temperature-controlling component is set to the outer packing group
Part outer surface, the measurement component, which is protected from light, to be set to the outer packing component internal and contacts with the temperature-controlling component inner surface,
The measurement component includes measuring cell, and the top of the measuring cell is equipped with for reading the photomultiplier tube of fluorescence, outer bottom is set
Separable suction pearl magnet can be bonded therewith, is internal equipped with positive and negative electrode by having, and the measuring cell is also connected with inlet tube and out liquid
Pipe, the inlet tube and outlet tube are protected from light and stretch out in the outsourcing arrangement.
Preferably, the fluid path unit include respectively with the sampling needle, post-processing needle connect syringe, hydraulic pump,
Hydraulic valve, liquid line, and connect with measuring unit for negative pressure pump, the waste liquid for collecting waste liquid needed for waste liquid is discharged
Tank.
Preferably, the display unit and the board control unit circuit connection, including tangibly display screen, use
In display screen outer cover, display screen shaft, display screen support rod and the scope rack of installing the display screen.
Preferably, the board control unit is equipped with central processing element and is equipped with system software, the board control
Unit can concentrate the movement of remaining each unit of coordinated control, and the signal that the measurement analytical unit measures can be carried out processing and
Processing result is sent to display unit.
The present invention provides a kind of measurement analysis methods of small electrical chemical illumination immunity analysis instrument, including following measurement point
Analyse step:
(1) measurement prepares: starting analyzer, by system initialization；Special agent needed for measuring is loaded to special agent
In the dedicated kit of warehouse receipt member, and magnetic bead stirring is carried out by mixing mechanism；Common reagent needed for measurement is loaded to logical
With in the universal kit of reagent unit；Cup group will be incubated for be put into the incubation disk of hatch unit；The cuvette cartridge of sample will be housed
In the sample rack for entering sample storehouse unit, sample rack is then pushed into sample storehouse unit, input sample bar code；
(2) starting measurement: start measurement button in display unit, analyzer enters following all automatic measurement program；
(3) incubation step one: sample needle extracts required special agent one from dedicated kit automatically by measure the item, then
Sample needle is cleaned by the sample service sink component being equipped in sample storehouse unit, it is rear to draw the sample put in sample storehouse unit
Then sample in test tube is added and is incubated in cup group in available incubation cup, be incubated for disk and carry out 38 DEG C to the liquid being incubated in cup
Incubation reaction is kept the temperature, incubation step one is reaction timing by the system software installed in board control unit；
(4) incubation step two: after incubation step one, dedicated examination needed for sample needle is extracted from dedicated kit automatically
Agent two, addition is completed in the incubation cup of incubation step one, and carries out compressing mixing, is incubated for disk and carries out to the liquid being incubated in cup
38 DEG C of heat preservation incubation reactions, incubation step two are reaction timing by the system software installed in board control unit；
(5) Beads enrichment: measurement analytical unit is automatically performed measuring cell preparation before the completion of incubation step two；It is incubated for
After the completion of step 2, post-processing needle automatically extends into the sample for being incubated for and drawing in cup and incubation reaction being completed, and incubation reaction is completed
Sample along with post-processing needle connect pipeline inflow measuring cell in；The oscillating motor that measurement analytical unit is equipped with drives therewith
The swing rod of connection makes the suction pearl magnet being arranged on swing rod fit to measuring cell outer bottom, the magnetic bead in the sample of incubation is completed
By magneticaction, it is adsorbed on measurement bottom of pond portion；Then post-processing needle extracts common reagent one to measuring cell automatically, and rinses magnetic
Pearl；Keep liquid in measuring cell static after completing magnetic bead cleaning；Pearl magnet clear point is inhaled in oscillating motor release；
(6) measurement analysis: being equipped with positive and negative electrode in measuring cell, is powered to positive and negative electrode, contained on magnetic bead in measuring cell
Shiner generates fluorescence because being powered, and photomultiplier tube is converted into electric signal after receiving fluorescence, measures analytical unit for the telecommunications
Number be analyzed and processed, obtain uploading to the system software processing installed in board control unit after signal value, processing result from
It is dynamic to be shown in display unit；
(7) electrode restores: post-processing needle extracts common reagent two automatically and cleans measuring cell and its pipeline, in measuring cell just
Negative electrode is powered up reversely, and restores electrode；
(8) measuring unit resets: the waste liquid generated after measuring cell is measured is discharged in the waste liquid tank that fluid path unit is equipped with, after
Processing needle extracts common reagent one again and fills measuring unit associated pipe and prepare next wheel measurement；
(9) measurement terminates: measurement terminates, and analyzer is in standby.
Beneficial effects of the present invention are as follows:
Small electrical chemical illumination immunity analysis instrument provided by the invention is the small department for small hospital, large hospital
And the design of the center She Kang.The analyzer, can be respectively by sample by sample needle and post-processing needle on mechanical arm and mechanical arm
This and reagent are accurately drawn and are added to be incubated in cup and be incubated for, and the sample after incubation is drawn into totally enclosed survey
Amount analytical unit measures analysis, entire to be incubated for, measure analytic process high degree of automation, does not need human assistance, reduces
Human error, it is easy to operate, greatly improve working efficiency；The incubation disk of the analyzer is simulated equipped with temperature control heating diaphragm
Human body temperature environment carries out sample incubation, to improve the accuracy of measurement result；The analyzer special agent and general examination
Agent uses low-temperature preservation, can be placed in instrument and measure at any time for a long time；The analyzer measures analytical unit using full envelope
It closes and pipeline sample introduction, effectively prevents the risk of open mould group light leakage and photomultiplier tube exposure under ambient light；The analysis
Instrument uses electrochemical luminescence reaction principle, with excellent measurement accuracy, it can be achieved that the immunization program huge number of analysis；This point
The touch display screen that analyzer is equipped with can directly carry out human-computer interaction, simple to operate, facilitate full-automatic realization.
Detailed description of the invention
Fig. 1 is the complete machine structure figure one of the embodiment of the present invention；
Fig. 2 is the complete machine structure figure two of the embodiment of the present invention
Fig. 3 is the special agent cellular construction figure one of the embodiment of the present invention；
Fig. 4 is the structure chart of the dedicated kit of the embodiment of the present invention；
Fig. 5 is the three-dimensional view of magnetic bead bottle in the dedicated kit of the embodiment of the present invention；
Fig. 6 is the three-dimensional view of reagent bottle in the dedicated kit of the embodiment of the present invention；
Fig. 7 is the special agent cellular construction figure two of the embodiment of the present invention；
Fig. 8 is the special agent cellular construction figure three of the embodiment of the present invention；
Fig. 9 is the cross-sectional view of the first temperature control refrigerating plant of the embodiment of the present invention；
Figure 10 is the sample storehouse cellular construction figure of the embodiment of the present invention；
Figure 11 is the sample rack structure chart of the sample storehouse unit of the embodiment of the present invention；
Figure 12 is the hatch unit structure chart one of the embodiment of the present invention；
Figure 13 is the hatch unit structure chart two of the embodiment of the present invention；
Figure 14 is the hatch unit structure chart three of the embodiment of the present invention；
Figure 15 is the incubation cup group structure chart of the hatch unit of the embodiment of the present invention；
Figure 16 is the incubation cup group structural exploded view of the hatch unit of the embodiment of the present invention；
Figure 17 is the common reagent cellular construction figure of the embodiment of the present invention；
Figure 18 is the mechanical arm cellular construction figure of the embodiment of the present invention；
Figure 19 is the display cell structure figure of the embodiment of the present invention；
Figure 20 is the measurement analytical unit structure chart of the embodiment of the present invention；
Figure 21 is the measurement analytical unit structural exploded view of the embodiment of the present invention；
Figure 22 is the measurement assembly assumption diagram of the measurement analytical unit of the embodiment of the present invention；
Figure 23 is the fluid path cellular construction figure of the embodiment of the present invention；
Figure 24 is the measurement analytical procedure flow chart of the embodiment of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.
As shown in Figures 1 and 2, the embodiment of small electrical chemical illumination immunity analysis instrument of the present invention, including rack 1, rack 1
Including the platform 11 that bottom is equipped with, the front of platform 11, which is successively arranged side by side, to be had special agent unit 2, sample storehouse unit 3, is incubated for
Unit 4, common reagent unit 5；The back upper place of rack 1 or platform 11 is equipped with upper mounting plate 12, and upper mounting plate 12 is equipped with mechanical arm list
Member 6；The front side of platform 11 is provided with display unit 7；The side back upper place of rack 1 is provided with measurement analytical unit 8；
The side back lower place of rack 1 is provided with fluid path unit 9；The 1 side back lower place of rack and positive back upper place are provided with board control unit
10, the circuit connection of board control unit 10 and remaining each unit described above, and it is equipped with central processing element (not shown)
And it is mounted with system software for controlling the automatic running of remaining each unit movement and complete machine.Above-mentioned each unit is arranged,
Limited space is made full use of, so that analyzer of the present invention is in the case where guaranteeing full-automatic function, compact-sized, volume reduces
Use easy to carry.
As shown in Fig. 1, Fig. 3, Fig. 7, Fig. 8, special agent unit 2 includes dedicated kit 210, dedicated kit mounting groove
204, for stirring the mixing mechanism of special agent and for the first temperature control refrigerating plant of low-temperature preservation dedicated kit 210
220, special agent unit 2 is mounted on the platform 11 of rack 1 by special agent unit mounting post 203.
As shown in Fig. 4-Fig. 8, dedicated kit 210 include box body 213, magnetic bead bottle 211, reagent bottle 212, magnetic bead bottle 211,
Reagent bottle 212 is placed in box body 213 vertically side by side, and magnetic bead bottle 211, reagent bottle 212 are provided with different special agents respectively.
Magnetic bead bottle 211 is freely rotatable in box body 213, and magnetic bead bottle 211 includes cylindric magnetic bead bottle main body 2110, magnetic bead bottle main body
2110 lower parts are equipped with driving section 2111, and driving section 2111 is exposed to box body 213, and driving section 2111 specifically can be magnetic bead bottle surface
Gear, groove or the friction block (not shown) being equipped with.Mixing mechanism includes mixing motor 201, mixing driving member 202, is mixed
Even motor 201 drives coupled mixing driving member 202, and the driving section 2111 mixed on driving member 202 and magnetic bead bottle 211 connects
It connects, therefore mixing motor 201 can drive magnetic bead bottle 211 in dedicated kit 210 to carry out reciprocating rotation therewith, carry out special agent
It mixes, avoiding special agent that precipitating occurs leads to density unevenness.Mixing driving member 202 specifically can be by eccentric wheel assembly and transmission
(not shown) is constituted, and transmission item is directed to for driving section 2111, can be rack gear, groove item either fricting strip.
As shown in Fig. 7, Fig. 8, Fig. 9, the first temperature control refrigerating plant 220 is installed on several mounting grooves of dedicated kit 210
Under 204, temperature control refrigeration is carried out；Dedicated kit mounting groove 204 be equipped with can thermally conductive (i.e. conduction refrigerating capacity) bottom plate 221, bottom
221 lower part of plate is successively arranged refrigeration conductive plate 222, semiconductor refrigeration chip 223, fixed plate 224, cooling fin 225 and fan
226；Fixed plate 224 is fixed on bottom for will freeze conductive plate 222, semiconductor refrigeration chip 223, cooling fin 225 and fan 226
The lower part of plate 221；Semiconductor refrigeration chip 223 includes cold and hot two sides, and hot face is contacted with cooling fin 225, and huyashi-chuuka (cold chinese-style noodles) connection refrigeration passes
Guide plate 222, refrigeration conductive plate 222 and 221 lower contacts of bottom plate, the top of bottom plate 221 are dedicated kit mounting groove 204.The
When one temperature control refrigerating plant 220 works, 223 huyashi-chuuka (cold chinese-style noodles) of semiconductor refrigeration chip generates cooling capacity, and cooling capacity is through semiconductor refrigeration chip
223, freeze conductive plate 222, the conduction of bottom plate 221 to dedicated kit mounting groove 204, make dedicated kit mounting groove 204, dedicated
Kit 210 and its interior special agent, which generate cold storage effect and automatically adjust, to be stablized at a temperature of certain；Semiconductor refrigerating simultaneously
The hot face of chip 223 generates heat, and heat is through cooling fin 225 and the conduction of fan 226 into air.
As shown in Figure 1, Figure 10 shows, sample storehouse unit 3 is mounted on the platform 11 of rack 1 by sample storehouse unit mounting post 302
On, sample storehouse unit 3 is used to carry out the input of sample with user interface, and sample storehouse unit 3 includes sample rack 310, sample rack peace
Fill seat 301, sample rack positioning device 303 and service sink component 304.In the present embodiment, sample rack mounting base 301 has for one to be led
The mounting plate of slot 305, sample rack positioning device 303 are a positioning resilient plunger.Sample is loaded in sample tube (not shown),
Sample tube is placed in sample rack 310, and sample rack 310 is inserted into the guide groove 305 of sample rack mounting base 301, positioning elastic column
Plug 303 is set in guide groove 305, for ensuring that sample rack 310 is inserted into guide groove 305 to designated position, and has fixed position
Effect, make sample rack 310 avoid moving in place afterwards, be conducive to sample needle and accurately protrude into sample tube to be sampled.
As shown in figure 11, be the schematic construction of sample rack 310, sample rack 310 have the hand-held part 31A that can grasp manually and
Place the slot 31B of sample tube.
As shown in Figure 10, sample service sink component 304 is used for that clean sample needle 605(as shown in figure 18) it is clear after sampling
It washes, is installed on 301 rear portion of sample rack mounting plate, sample service sink component 304 includes service sink (not shown), detergent line
(not shown), 304 bottom of sample service sink component are equipped with waste liquid outlet (not shown) and are connected to fluid path unit 9, and cleaning produces
Raw waste liquid is discharged into the waste liquid tank (not shown) of fluid path unit 9 by pipeline.Service sink top is equipped with ejected wash water water outlet (figure
In do not show), ejected wash water by water outlet flow out, 605 inside and outside wall of sample needle can be cleaned.
As shown in Fig. 1, Figure 12-Figure 14, hatch unit 4 sets bracket 400, and bracket 400 is pacified by hatch unit mounting post 407
On the platform 11 of rack 1.Bracket 400 is equipped with rotatable incubation disk 405, and incubation motor is arranged below in bracket 400
401, synchronous belt 401A is incubated for disk 405 to drive, and putting in disk has the incubation cup groups 403 for integrating multiple incubation cups 432,
It is incubated for 405 lower part of disk and is equipped with temperature control heating diaphragm 406, temperature control heating diaphragm 406 can be to incubation disk 405 and its interior incubation cup
Group 403 is heated, and temperature is controlled at about 38 DEG C, is simulated human body temperature environment, is made the incubation reaction liquid being incubated in cup 432
Carry out good incubation reaction.Incubation disk 405, which can be rotated to different incubation cups 432, can cooperate addition with sample needle 605
Different samples and reagent is reacted simultaneously.
As shown in Figure 12 and Figure 13, being incubated for disk 405, box-like, top is equipped with incubation disk cover 409 in circle, and being incubated for disk cover 409 can prevent
Only sundries falls into be incubated in disk 405 or fall into and be incubated in cup 432, while also having certain heat insulation effect.It is incubated on disk cover 409
Equipped with incubation cup group entrance 4091 and it is equipped with adding mouth 4092, being incubated for interior be equipped with of disk 405 can be with an a pair with incubation cup 432
Ying Bingke accommodates the incubation hole 4051 for being incubated for cup 432.It is incubated for cup group entrance 4091 and is incubated for cup group to facilitate loading or take out,
Adding mouth 4092 is as shown in figure 18 to facilitate sample needle 605 or post-processing needle 608() it passes through, so that it is can be plugged into incubation cup
To extract or injecting sample, reagent or reaction solution in 432.
It as shown in Figure 12, Figure 15, Figure 16, is incubated in disk 405 and four groups of incubation cup groups 403 can be placed, being incubated for cup group 403 includes
The cricoid cup holder 431 of quadrant and vertical sleeve are incubated for cup 432 and set in several identical incubation cups 432 on cup holder 431
There is the cup inner cavity 4320 that can contain reaction solution, being incubated for cup 432 includes upper cup body 4321 and lower cup body 4322, and upper cup body 4321 is in pipe
Shape, it is circular arc that the inside and outside wall of lower cup body 4322, which is received after upper cup body 4321 in the inverted conical shape and bottom transition that are gradually reduced,
Shape, cup holder 431 are equipped with glass holder hole 4310, and unremovably sleeve makes to be incubated for cup upper cup body 4321 in glass holder hole 4310
432 fix as one with cup holder 431, and lower cup body 4322 is exposed to cup holder 431 in order to so that being incubated for cup 432 is inserted into an a pair
In the incubation hole 4051 answered.It is incubated for cup group 403 and integrates multiple incubations cups 432, and cup 432 is affixed with cup holder 431 is for incubation
One, can facilitate the packed and transported and transportation safety for being incubated for cup 432 and being incubated for cup group 403, and another replacement is incubated for fast high when cup 432
Effect.
As shown in figures 12-16, in the present embodiment, the heating that hatch unit 4 is used to carry out sample is incubated for work, with reagent
Mixed sample, which is added, to be incubated in cup 432, and incubation cup group 403 is placed in incubation disk 405 and is incubated in hole 4051 accordingly, each
It is incubated for the corresponding incubation hole 4051 for being incubated for disk 405 of cup 432.Being incubated for motor 401 drives the incubation rotation of disk 405 to cooperate machine
The corresponding actions of tool arm unit 6.Temperature control heating diaphragm 406, which is installed on to be incubated for below disk 405, adds the incubation progress temperature control of disk 405
Heat.It is incubated for disk and is maintained at 38 DEG C or so work, need to simulate human body environment because being incubated for disk, to ensure antigen in being immunoreacted and resist
Effective combination of body.
As shown in figure 14, it is incubated for disk 405 and is equipped with the code-disc 408 that incubation disk 405 can be followed to rotate together, code-disc further below
408 for one be equipped with code tooth turntables, 408 lower part of code-disc be equipped with detection code disk initial position code-disc zero-bit inductor 411 and can
The code-disc rotation precision inductor 412 of sensing code tooth variation.
As shown in Figure 12-Figure 14, in the present embodiment, hatch unit 4 is detected using code-disc 408, rotation precision inductor 412
Code-disc code tooth rotational angle and the mode for carrying out signal feedback, come control be incubated for disk 405 rotation precision to be accurately loaded,
Principle is the teeth portion gap that each is incubated for the corresponding code-disc 408 in hole 4051, if there are micro step-outs to make in certain rotation
At error or reaction gap error, will not this error accumulation to rotating next time, i.e., rotate every time it is relatively independent, because
This, which ensure that, is incubated for the precision that disk 405 rotates, and the sample needle 605 and post-processing needle 608(for ensuring that mechanical arm unit 6 are such as
Shown in Figure 18) it can accurately be inserted into each the incubation cup 432 for being incubated for cup group 403.
As shown in Fig. 1, Figure 12, in the present embodiment, hatch unit 4, which is additionally provided with, is incubated for cleaning assembly 402 and wash bowl 404,
It is identical as sample service sink 304 structures of component to be incubated for service sink component 402() it is installed on 405 right side of incubation disk, pass through cleaning pipe
Road is connected with fluid path unit.Wash bowl 404, which is installed on, is incubated for 405 front of disk.Being incubated for service sink component 402 is post-processing needle 608
The place that (as shown in figure 18) is routinely cleaned, being incubated for service sink component 402 can be by being added ejected wash water in post-processing needle 608
Outer wall carries out conventional cleaning, and the waste liquid for cleaning generation enters the waste liquid tank (not shown) of fluid path unit 9 by pipeline.Wash bowl
404 are used for the strong cleaning solution reagent bottle (not shown) of storage system, and system must clean by force item finishing high level sample or use
When purpose, it should as shown in figure 18 to sample needle 605(using strong cleaning solution) it cleans.Strong cleaning solution is in use, by adding
Sample needle 605 is drawn and is stored in inside sample needle 605, and the waste liquid for cleaning generation is drained by sample service sink component 304.
As shown in Fig. 1, Figure 17, common reagent unit 5 is mounted on the flat of rack 1 by common reagent unit mounting post 504
On platform 11, common reagent unit 5 includes the second temperature that universal kit 501, universal kit mounting groove 502 and its underpart are equipped with
Device for cooling 503 is controlled, common reagent unit 5 is used to carry out common reagent storage and temperature control acts on, and universal kit 501 is placed
In in universal kit mounting groove 502, the second temperature control refrigerating plant 503(structure is identical as the first temperature control refrigerating plant 220) peace
Loaded on 502 lower section of universal kit mounting groove to carry out temperature control storage to common reagent.
As shown in Fig. 1, Figure 18, the back upper place of platform 11 is equipped with upper mounting plate 12, and upper mounting plate 12 is equipped with mechanical arm unit 6,
Mechanical arm unit 6 includes the first mechanical arm 61 with sample needle 605 and the second mechanical arm 62 with post-processing needle 608, first
Mechanical arm 61, second mechanical arm 62 drive respectively under, sample needle 605 can special agent unit 2, sample storehouse unit 3, be incubated for
The top of unit 4 moves freely, and post-processing needle 608 can be moved freely in the top of hatch unit 4, common reagent unit 5.It is mechanical
Arm unit 6 is used to carry out taking liquid, liquid relief to act on to each sample and reagent, incubation reaction liquid etc..
As shown in figure 18, in the present embodiment, the first mechanical arm 61 with sample needle 605 is three-dimensional arm, band post-processing needle 608
Second mechanical arm 62 be two-dimentional arm, three-dimensional arm 61 and two-dimentional arm 62 are mounted on the same root X-direction that upper mounting plate 12 is equipped with and lead
The both ends of rail 601, three-dimensional arm 61 include the three-dimensional Y-axis arm 602 that can move freely on X-direction guide rail 601, are mounted on three-dimensional Y
The three-dimensional Z axis arm 603 that can move freely on armshaft 602 along Y-direction, sample needle 605 are parallelly mounted on three-dimensional Z axis arm 603 simultaneously
It can be moved freely along Z-direction；Two-dimentional arm 62 includes the two-dimentional Y-axis arm 606 that can move freely on X-direction guide rail 601, fixed peace
Two-dimentional Z axis arm 607 on two-dimentional Y-axis arm 606, post-processing needle 608 are parallelly mounted on two-dimentional Z axis arm 607 and can be along the sides Z
To moving freely.The movement of three-dimensional arm 61 and two-dimentional arm 62 on each movable direction is respectively by respective motor and synchronous belt
Driving, such as three-dimensional arm 61 is in the X direction, drives synchronous belt 611 that three-dimensional Y-axis arm 602 is driven to lead in X-direction equipped with motor 610
It is moved left and right on rail 601.
As shown in Fig. 1, Figure 19, display unit 7 and 10 circuit connection of board control unit, for being carried out with user
Human computer interaction, display unit 7 include tangibly display screen 703, the display screen outer cover for installing display screen 703
704, display screen shaft 702, display screen support rod 705 and scope rack 701.Display screen 703 is installed on display screen outer cover 704
In, display screen outer cover 704 can rotate display screen 703 by display screen shaft 702, adjust to adaptation user and use and see
The possible angle seen.Display screen support rod 705 can be fixed after display screen 703 turns to possible angle.Display unit 7
It is installed in rack 1 entirely through scope rack 701.
As shown in Figure 1, Figure 2, shown in Figure 20, the measurement analytical unit connecting with 5 fluid path of common reagent unit is additionally provided in rack 1
8, measurement analytical unit 8 includes for measuring the measurement component 80 of the sample of incubation reaction, outsourcing arrangement 81, for controlling
The temperature-controlling component 82 and analysis processing circuit (not shown), temperature-controlling component 82 for measuring component temperature are set to outer packing group
The outer surface of part 81, measurement component 80, which is protected from light, to be set to 81 inside of outsourcing arrangement and contacts with 82 inner surface of temperature-controlling component, is surveyed
Measuring component 80 includes measuring cell 801, and the top of measuring cell 801 is equipped with the photomultiplier tube (PMT) 802 for reading fluorescence, is surveyed
The outer bottom in amount pond 801 is equipped with can be bonded separable suction pearl magnet 803 therewith, and measuring cell 801 is connected with inlet tube 804 and goes out
Liquid pipe 805, inlet tube 804 and outlet tube 805, which are protected from light, stretches out in outsourcing arrangement 81.
As shown in figure 22, in the present embodiment, measurement component 80 is by framework based on dilatory substrate 800, the survey that liquid passes through
Amount pond 801 is mounted on dilatory substrate 800 in the following, the photomultiplier tube (PMT) 802 for reading fluorescence is accurately installed on measuring cell
Right above 801, photomultiplier tube (PMT) 802 is externally provided with casing 808 to be protected from light.The lower surface of measurement component 80 is equipped with pendulum
Linkage drives swing rod 807 by oscillating motor 806, to fit to or divide the suction pearl magnet 803 that 807 end of swing rod is arranged
Leave 801 outer bottom of measuring cell.Measuring cell is reached when the sample of incubation reaction enters measurement analytical unit 8 by inlet tube 804
When 801,806 swing rod 807 of oscillating motor will inhale pearl magnet 803 and fit to 801 outer bottom of measuring cell, have marker in sample
Magnetic bead is sucked by pearl magnet 803 is inhaled.It is additionally provided with positive and negative electrode inside measuring cell 801, when electrode is powered, with label in sample
The magnetic bead of object, that is, capable of emitting week fluorescent, photomultiplier tube (PMT) 802 works at this time, reads what magnetic bead in a period of time issued
Week fluorescent.
As shown in Fig. 2, Figure 23, fluid path unit 9 is flowed to for controlling sample and reagent and waste liquid, and fluid path unit 9 is installed on
On bracket 901, bracket 901 is installed in rack 1 to ensure whole property easy to maintain.Fluid path unit 9 include respectively with sampling needle
605, syringe 902, hydraulic pump 903, hydraulic valve 904 that post-processing needle 608 connects, and connect with measuring unit 8 for arranging
Negative pressure pump (not shown) needed for waste liquid, the waste liquid tank (not shown) for collecting waste liquid out.
The above, special agent refer to the reagent of immune response project, in usual each project, dedicated kit it is molten
Liquid capacity is not quite similar, and the reagent reaction principle of specific dosage and the serving as reference project, special agent is in use, by sample needle
605 draw, and are respectively added to be incubated for cup with sample.Common reagent refers to that the needs such as all items measurement, instrument initialization make
System reagent.Common reagent belongs to the exclusive reagent of measurement analytical unit 8, is drawn by post-processing needle 608.
As shown in Fig. 1-Figure 22, three-dimensional arm 61 can drive sample needle 605 respectively by the special agent in special agent unit 2
It is filled into after being drawn respectively with the sample in sample storehouse unit 3 in the incubation cup 432 in hatch unit 4 and carries out incubation reaction, two
Dimension arm 62 can drive post-processing needle 608 will be general in the sample and common reagent unit 5 that are incubated for the incubation reaction in cup 432
It is reacted in the measuring cell 801 that reagent is drawn onto measurement analytical unit 8 by pipeline.
As shown in figure 24, the measurement analysis method of small electrical chemical illumination immunity analysis instrument of the present invention, including it is following
Measure analytical procedure:
(1) measurement prepares: starting analyzer, by system initialization；Special agent needed for measuring is loaded to special agent
In the dedicated kit 210 of warehouse receipt member 2, and magnetic bead stirring (avoiding precipitating) is carried out by mixing mechanism；Needed for measuring
Common reagent load into the universal kit 501 of common reagent unit 5；Cup group 403 will be incubated for and be put into incubating for hatch unit 4
It educates in disk 405；Test tube equipped with sample is fitted into sample rack 310, sample rack 310 is then pushed into sample storehouse unit 3, input
Catalogue number's bar code；
(2) starting measurement: start measurement button in display unit 7, analyzer enters following all automatic measurement program；
(3) incubation step one: sample needle 605 extracts required special agent from dedicated kit 210 automatically by measure the item
One, then sample needle 605 is cleaned by the sample service sink component 304 being equipped in sample storehouse unit 3, draws sample afterwards
Then sample in test tube is added and is incubated in cup group 403 in available incubation cup 432, is incubated in 405 pairs of incubation cups 432 of disk
Liquid carries out 38 DEG C of heat preservation incubation reactions, and incubation step one is fixed for reaction by the system software installed in board control unit 10
When；
(4) incubation step two: after incubation step one, needed for being extracted in the automatic dedicated kit 210 of sample needle 605 specially
With reagent two, addition is completed in the incubation cup 432 of incubation step one, and carries out compressing mixing, is incubated for 405 pairs of incubation cups of disk
Liquid in 432 carries out 38 DEG C of heat preservation incubation reactions, and incubation step two is by the system software installed in board control unit 10
Reaction timing；
(5) Beads enrichment: measurement analytical unit 8 is automatically performed 801 preparation of measuring cell before the completion of incubation step two；
After the completion of incubation step two, post-processing needle 608 automatically extends into the sample for being incubated for and drawing in cup 432 and incubation reaction being completed, complete
It is flowed into measuring cell 801 at the sample of incubation reaction along the pipeline being connect with post-processing needle 608；Measure the pendulum of analytical unit 8
Dynamic motor 806 drives swing rod 807, and the suction pearl magnet 803 being set on swing rod 807 is made to fit to 801 outer bottom of measuring cell, complete
At the magnetic bead in the sample of incubation reaction by magneticaction, it is adsorbed on 801 bottom of measuring cell；It post-processes needle 608 and extracts automatically and lead to
With reagent one (BUFFER) to measuring cell 801, and rinse magnetic bead；Keep liquid in runner static after completing magnetic bead cleaning；Release
Inhale 803 clear point of pearl magnet；
(6) measurement analysis: 801 electrode of measuring cell is powered, and contained shiner generates fluorescence, photomultiplier tube on magnetic bead
802 receive and are converted into electric signal after fluorescence, which is analyzed and processed by measurement analytical unit 8, after obtaining signal value
The system software processing installed in board control unit 10 is uploaded to, processing result is shown in display unit 7 automatically；
(7) electrode restores: post-processing needle 608 extracts common reagent two (AFFER) cleaning measuring cell 801 and its pipe automatically
Road, 801 electrode of measuring cell are powered up reversely, and restore electrode；
(8) measuring unit resets: the waste liquid that the measurement of measuring cell 801 generates is discharged in the waste liquid tank of fluid path unit 9, it is rear to locate
Reason needle 608 extracts common reagent one (BUFFER) filling 8 associated pipe of measuring unit again and prepares next wheel measurement；
(9) measurement terminates: measurement terminates, and analyzer is in standby.
Described above is only presently preferred embodiments of the present invention, and above-mentioned specific embodiment is not limitation of the present invention.
In technical idea scope of the invention, various modifications and modification can occur, all those skilled in the art according to
Upper description done retouching, modification or equivalent replacement, belong to the range that the present invention is protected.
Claims (15)
1. a kind of small electrical chemical illumination immunity analysis instrument is equipped with the rack of platform including bottom, it is characterised in that: the platform
It is equipped with special agent unit, sample storehouse unit, hatch unit, common reagent unit；The special agent unit includes dedicated
Kit, dedicated kit mounting groove, the mixing mechanism for stirring special agent and for low-temperature preservation dedicated kit
First temperature control refrigerating plant；The hatch unit includes the incubation disk rotated by incubation motor driven, is incubated for the collection put in disk
It is multiple to be incubated for the incubation cup group and be incubated for the temperature control heating diaphragm that disk lower part is equipped with that cup is integrated；The common reagent unit includes
The second temperature control refrigerating plant that universal kit, universal kit mounting groove and its underpart are equipped with；The rack back upper place is equipped with
Upper mounting plate, the upper mounting plate are equipped with mechanical arm unit, the machinery arm unit include first mechanical arm equipped with sample needle and
Second mechanical arm equipped with post-processing needle, the sample needle can be in the special agent lists under first mechanical arm drive
It is moved freely above member, sample storehouse unit, hatch unit, the post-processing needle can be described under second mechanical arm drive
It is moved freely above hatch unit, common reagent unit；Be additionally provided in the rack measurement analytical unit, for control sample with
Reagent flow direction and collect the fluid path unit of waste liquid, the display unit for carrying out human-computer interaction and with remaining described each unit
Circuit connection is used for central controlled board control unit.
2. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the first temperature control refrigeration
Device or the second temperature control refrigerating plant, including bottom plate that can be thermally conductive, the bottom plate is equipped with refrigeration conductive plate, semiconductor in turn below
Refrigerating chip, cooling fin and fan, the semiconductor refrigeration chip include cold and hot two sides, the hot face and cooling fin one side
It is fitted and connected, the fan is connect with the cooling fin another side to radiate, the huyashi-chuuka (cold chinese-style noodles) and refrigeration conductive plate one side
It is fitted and connected, the refrigeration conductive plate another side is fitted and connected with the bottom plate lower plane, and plane is equipped with described on the bottom plate
Dedicated kit mounting groove or universal kit mounting groove.
3. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the dedicated kit packet
Magnetic bead bottle, the reagent bottle for including box body, putting in box body are respectively provided with special agent, the magnetic bead in the magnetic bead bottle, reagent bottle
Bottle is freely rotatable, and its underpart is equipped with driving section and is exposed to the box body；The mixing mechanism includes mixing motor, mixing biography
Moving part, the mixing driving member are connect with the driving section of the magnetic bead bottle, and the mixing motor can drive the mixing driving member
The magnetic bead bottle is driven to carry out reciprocating rotation.
4. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the sample storehouse unit packet
Include sample rack, sample rack mounting base, sample rack positioning device and the sample service sink component for putting sample tube, the sample
This service sink component includes sample service sink, the detergent line being connected with the fluid path unit, waste liquid discharge line.
5. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the incubation disk is in circle box
Shape, internal be equipped with correspond with the incubation cup and can accommodate the incubation hole for being incubated for cup, put four in the incubation disk
Group the incubations cup group, the incubation cup group includes the cricoid cup holder of quadrant and vertical sleeve incubates in several on cup holder
Educate cup.
6. small electrical chemical illumination immunity analysis instrument according to claim 5, it is characterised in that: the incubation cup is equipped with can
Contain the cup inner cavity of reaction solution, the incubation cup includes upper cup body and lower cup body, the upper cup body in a tubular form, the lower cup body
It is circular shape that inside and outside wall, which is received after the upper cup body in the inverted conical shape and bottom transition that are gradually reduced, on the cup holder
Equipped with glass holder hole, unremovably for sleeve in the glass holder hole, the lower cup body is exposed to the cup holder to the upper cup body.
7. small electrical chemical illumination immunity analysis instrument according to claim 5, it is characterised in that: the incubation disk top is set
There is incubation disk cover, the disk cover that is incubated for is equipped with adding mouth, the entrance for being packed into and taking out incubation cup group.
8. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the hatch unit is also wrapped
The code-disc with the incubation disk coaxial arrangement is included, the code-disc lower part is equipped with code-disc zero-bit inductor and code-disc rotation precision incudes
Device.
9. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the hatch unit is also wrapped
Include and be incubated for cleaning assembly and wash bowl, the incubations cleaning assembly include incubation service sink, be connected with the fluid path unit it is clear
Pipeline, waste liquid discharge line are washed, the wash bowl is used for the strong cleaning solution reagent bottle of storage system.
10. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the first mechanical arm
For three-dimensional arm, the second mechanical arm is two-dimentional arm, the three-dimensional arm and two-dimentional arm be mounted on the upper mounting plate be equipped with it is same
On a piece X-direction guide rail, the three-dimensional arm includes the three-dimensional Y-axis arm that can move freely on X-direction guide rail, in the three-dimensional Y
The three-dimensional Z axis arm that can move freely along Y-direction installed on armshaft, the sample needle are parallelly mounted on the three-dimensional Z axis arm
And it can be moved freely along Z-direction；The two dimension arm includes the two-dimentional Y-axis arm that can move freely on X-direction guide rail, described two
The two-dimentional Z axis arm being fixedly mounted on dimension Y-axis arm, the post-processing needle are parallelly mounted on the two-dimentional Z axis arm and can be along Z-directions
It moves freely；The movement of the three-dimensional arm and two-dimentional arm on each movable direction is driven by respective motor and synchronous belt respectively
It is dynamic.
11. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the measurement analysis is single
Member includes for measuring the measurement component for being incubated for sample, outsourcing arrangement, the temperature control for controlling the measurement component temperature
Component, analysis processing circuit, the temperature-controlling component are set to the outer packing module outer surface, and the measurement component is protected from light setting
It is contacted in the outer packing component internal and with the temperature-controlling component inner surface, the measurement component includes measuring cell, the survey
The top in amount pond is equipped with for reading the photomultiplier tube of fluorescence, outer bottom be equipped with therewith can be bonded separable suction pearl magnet,
Inside is equipped with positive and negative electrode, and the measuring cell is also connected with inlet tube and outlet tube, and the inlet tube and outlet tube are protected from light stretching
In the outsourcing arrangement.
12. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the fluid path unit packet
Include syringe, hydraulic pump, hydraulic valve, the liquid line connecting respectively with the sample needle, post-processing needle, and and measuring unit
Connection is for negative pressure pump, the waste liquid tank for collecting waste liquid needed for waste liquid is discharged.
13. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the display unit
With the board control unit circuit connection, display screen outer cover including tangibly display screen, for installing the display screen,
Display screen shaft, display screen support rod and scope rack.
14. small electrical chemical illumination immunity analysis instrument according to claim 1, it is characterised in that: the board control is single
Member is equipped with central processing element and is equipped with system software, and the board control unit can concentrate remaining each unit of coordinated control
Movement, and the signal that the measurement analytical unit measures can be subjected to processing and processing result is sent to display unit.
15. a kind of measurement analysis method of small electrical chemical illumination immunity analysis instrument according to claim 1, feature exist
In including following measurement analytical procedure:
(1) measurement prepares: starting analyzer, by system initialization；Special agent needed for measuring is loaded to special agent warehouse receipt
In the dedicated kit of member, and magnetic bead stirring is carried out by mixing mechanism；Common reagent needed for measurement is loaded to general examination
In the universal kit of agent unit；Cup group will be incubated for be put into the incubation disk of hatch unit；Test tube equipped with sample is packed into sample
In the sample rack of this warehouse receipt member, sample rack is then pushed into sample storehouse unit, input sample bar code；
(2) starting measurement: start measurement button in display unit, analyzer enters following all automatic measurement program；
(3) incubation step one: sample needle extracts required special agent one from dedicated kit automatically by measure the item, then passes through
The sample service sink component being equipped in sample storehouse unit cleans sample needle, rear to draw the sample tube put in sample storehouse unit
In sample, be then added and be incubated in cup group in available incubations cup, be incubated for disk 38 DEG C are carried out to the liquid being incubated in cup and keep the temperature
Incubation reaction, incubation step one are reaction timing by the system software installed in board control unit；
(4) incubation step two: after incubation step one, special agent needed for sample needle is extracted from dedicated kit automatically
Two, addition is completed in the incubation cup of incubation step one, and carries out compressing mixing, is incubated for disk and carries out 38 to the liquid being incubated in cup
DEG C heat preservation incubation reaction, incubation step two is reaction timing by the system software installed in board control unit；
(5) Beads enrichment: measurement analytical unit is automatically performed measuring cell preparation before the completion of incubation step two；Incubation step
After the completion of two, post-processing needle automatically extends into the sample for being incubated for and drawing in cup and incubation reaction being completed, and the sample of incubation reaction is completed
This is flowed into measuring cell along the pipeline connecting with post-processing needle；The oscillating motor drive that measurement analytical unit is equipped with is attached thereto
Swing rod, so that the suction pearl magnet being arranged on swing rod is fitted to measuring cell outer bottom, the magnetic bead in the sample of incubation be completed by magnetic
Power effect, is adsorbed on measurement bottom of pond portion；Then post-processing needle extracts common reagent one to measuring cell automatically, and rinses magnetic bead；It is complete
Keep liquid in runner static after cleaning at magnetic bead；Pearl magnet clear point is inhaled in oscillating motor release；
(6) measurement analysis: being equipped with positive and negative electrode in measuring cell, is powered to positive and negative electrode, contained on magnetic bead in measuring cell to shine
Object generates fluorescence because being powered, and photomultiplier tube, which receives, is converted into electric signal after fluorescence, measurement analytical unit by the electric signal into
Row analysis processing uploads to the system software processing installed in board control unit after obtaining signal value, and processing result is shown automatically
It is shown in display unit；
(7) electrode restores: post-processing needle extracts common reagent two automatically and cleans measuring cell and its pipeline, to negative electricity positive in measuring cell
Pole is powered up reversely, and restores electrode；
(8) measuring unit resets: the waste liquid generated after measuring cell is measured is discharged in the waste liquid tank that fluid path unit is equipped with, post-processing
Needle extracts common reagent one again and fills measuring unit associated pipe and prepare next wheel measurement；
(9) measurement terminates: measurement terminates, and analyzer is in standby.
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