CN105628947A - Whole blood autoluminescence immunoassay point-of-care test instrument - Google Patents
Whole blood autoluminescence immunoassay point-of-care test instrument Download PDFInfo
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- CN105628947A CN105628947A CN201610071335.1A CN201610071335A CN105628947A CN 105628947 A CN105628947 A CN 105628947A CN 201610071335 A CN201610071335 A CN 201610071335A CN 105628947 A CN105628947 A CN 105628947A
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- 210000004369 blood Anatomy 0.000 title claims abstract description 25
- 239000008280 blood Substances 0.000 title claims abstract description 25
- 238000003018 immunoassay Methods 0.000 title claims abstract description 22
- 238000012123 point-of-care testing Methods 0.000 title abstract description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 72
- 239000007788 liquid Substances 0.000 claims abstract description 62
- 238000001514 detection method Methods 0.000 claims abstract description 53
- 238000005406 washing Methods 0.000 claims abstract description 39
- 239000000758 substrate Substances 0.000 claims abstract description 30
- 238000005070 sampling Methods 0.000 claims abstract description 28
- 238000006243 chemical reaction Methods 0.000 claims abstract description 20
- 238000003825 pressing Methods 0.000 claims abstract description 10
- 239000002699 waste material Substances 0.000 claims abstract description 10
- 238000002347 injection Methods 0.000 claims abstract description 4
- 239000007924 injection Substances 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 56
- 230000004807 localization Effects 0.000 claims description 17
- 230000007246 mechanism Effects 0.000 claims description 12
- 239000000376 reactant Substances 0.000 claims description 12
- 238000003860 storage Methods 0.000 claims description 8
- 238000009434 installation Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 3
- 238000012360 testing method Methods 0.000 abstract description 16
- 210000001124 body fluid Anatomy 0.000 abstract description 2
- 239000010839 body fluid Substances 0.000 abstract description 2
- 210000002381 plasma Anatomy 0.000 abstract description 2
- 210000002966 serum Anatomy 0.000 abstract description 2
- 210000000601 blood cell Anatomy 0.000 abstract 1
- 238000000034 method Methods 0.000 description 17
- 230000008569 process Effects 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000007689 inspection Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000004020 luminiscence type Methods 0.000 description 4
- 239000004005 microsphere Substances 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- ZBKIUFWVEIBQRT-UHFFFAOYSA-N gold(1+) Chemical compound [Au+] ZBKIUFWVEIBQRT-UHFFFAOYSA-N 0.000 description 1
- 210000003677 hemocyte Anatomy 0.000 description 1
- 229940000351 hemocyte Drugs 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a whole blood autoluminescence immunoassay point-of-care test instrument. The instrument sequentially comprises an integrated reagent tube storing and fetching device, an automatic sampling device, an item group parallel reaction treatment device, a washing and substrate adding device and a photon detection counter. By the aid of the item group parallel reaction treatment device, test items of any patient can be parallelly operated, detected and reported quickly by means of item integrated cards, so that test efficiency is improved. By intelligent and automatic sample adding, indexes of each item group can be tested parallelly, so that sample adding time is saved. By onsite detection for filter pressing and washing, operations required by each integrated reagent of each item group are handled automatically, and accordingly test efficiency is improved. By pulsed injection of washing liquor for washing and continuous pump drainage of waste liquid, quickness in washing is achieved, and integral operating speed is increased. By matching of the instrument and the integrated reagents, blood cells can be well separated, point-of-care test of a whole blood sample can be realized, and suitableness for samples of whole blood, serum, plasma, other body fluids and the like is realized.
Description
Technical field
The present invention relates to a kind of whole blood automatic luminous immunoassay instant detector, specifically a kind of can automatically detect multiple integrated Reagent Tube in the tray rack of some stackings and detect equipment with the luminescence immunoassay constituting the whole blood sample project team of multi objective, and the whole blood sample project team parallel reaction of some multi objective can be processed to improve detection efficiency by this equipment.
Background technology
Chemiluminescence technique because of its high sensitivity, wide linear analysis scope, good stability, and can automation mechanized operation, be the outstanding analytical tool of a class. Combine with high specific, highly sensitive heterogeneous immunoassay technology, constitute a class high specific, highly sensitive analysis platform, be widely used at modern medicine, life science.
At present, luminescence immunoassay technology trends is intended to set up the reagent of personalization, instrument integrated detection scheme, the two is highly merged to form unique advantage, plays the convenience in use and realize optimum performance, reaching best testing result.
In clinical medical inspection field, the developing trend of detection method in obtaining accurate assay when doctor, patient meet medical, and namely what is called is instant detects (PointofCareTest, POCT) method. This type of method one requires greatly to be able to detection whole blood sample, and therefore reagent and instrument integrated consider it is necessary, to get rid of the interference to testing result of the hemocyte composition.
In instant detection process, parallel parsing and simultaneously report multi objective extremely important. Multi objective can provide more, diagnostic message more accurately for doctor, makes correct diagnosis for doctor; Multi objective parallel parsing can meet better and immediately detects needs. In actually detected process, the test event of each patient, quantity all differ; The project team that each patient is detected as a unit must to this can synchronization process in groups, complete reaction, this is extremely important for immediately detecting.
In POCT class detection system, the test event of each patient, quantity all differ. How to realize integrated reagent store in seat in the plane and convenient take, it is achieved Synchronization Analysis, extremely important for realizing instant detection. There is the risk by mistake taking misuse in the integrated Reagent Tube of manual crawl; When specimen amount is big, because operator are in a rush, more add the risk gone wrong. Therefore, integrated Reagent Tube stores at machine and takes very necessary with intelligence, automatic quick.
Existing full-automatic immune detection instrument has two kinds to take pipe mode: the automatic tube cleaner sub-argument of general pipe and array capture vertical type reaction tube. Because the two reaction tube is open general detection pipe, without other outer package, take pipe process relatively easy.
Denmark's thunder degree AQT90FLEX immunity analysis instrument, the France BioMerieuxVidas of Mei Liai, Mitsubishi chemistry MitsubishiPATHFAST are instant detecting devices of class whole blood sample. This kind of device detects limited project combined test for specified disease. Due to machine limited bits, after one group of index test, then project team's test of other patients can only be started. One group of test is usually 16��20 minutes, and the waiting time is long, and efficiency is low, and full-page proof when being unfavorable for the relatively multisample such as outpatient service processes. It is more difficult to meet the combination in any project team detection needs of multiple disease many indexes.
It is that current one big class detects product immediately with nitrocellulose filter for solid phase lateral immunochromatography technique product. Simplicity is the feature that it is prominent. But, owing to material variation is big, process controllability is poor, detection process can not be washed, product quality poor controllability. Making this series products sensitivity low, precision is low, poor repeatability, and dosing accuracy is not enough, is only used for the primary dcreening operation to high-risk group, it is impossible to as the conventional tool of medical diagnosis on disease. With gold colloidal and fluorescent material for representative that label lateral chromatography product is this kind of technology.
With the multi-target analysis that lateral chromatography analytical technology is set up, a kind of method is independently to corresponding project team indicator reagent application of sample, after the standing and reacting regular hour, read its relevant signal value respectively, calculating each index concentration value, belong to scatter operation, standardization level is low. Another kind of multi objective Parallel analytic method is that tested project team index sample introduction end is centripetal concentrates on sample introduction zone, and after a loading, sample spreads to each index sample introduction end respectively, until completing detection. Later approach, due to the difference of each reagent sample introduction and agent structure, is difficult to determine the accuracy of its sample introduction.
Immunex system under U.S.'s Angen house flag is another kind of multi objective parallel parsing system, and this analysis system is that after multi objective solid phase microsphere and label being mixed, the sample of disposable addition certain volume mixes with it, washing microsphere after reaction certain time. Microsphere after having washed enters microchannel, by tally sheet its representative project of microsphere fluorescence information identification, then counts the fluorescent marker information content of reactant it on, thus the analysis of multi objective is detected by realization. Detecting instrument is required height by the method, and testing efficiency is low. Scientific research field for micro-example has certain use value, but high flux advantage is difficult to manifest in large batch of Clinical Laboratory works.
Summary of the invention
For solving the problems referred to above, it is contemplated that propose a kind of whole blood automatic luminous immunoassay instant detector, quickly to multiple disease, the combination of any project team of multi objective, parallel instant detection, to improve detection efficiency and to ensure sensitivity and the accuracy of assay.
For reaching above-mentioned purpose, the present invention provides a kind of whole blood automatic luminous immunoassay instant detector, including photon detection counting assembly, it is characterised in that it also sequentially includes:
Integrated Reagent Tube storage fetching is put, it includes placing a stand of some pallets for stacking, the building floor choosing device and for the pallet of any one layer being released or drawn in stand positions, for the some integrated Reagent Tube in the pallet to release stand, the three-dimensional localization pickup apparatus taken out, and loads in project integrated card by three-dimensional localization pickup apparatus by the integrated Reagent Tube taken out from pallet; Described integrated Reagent Tube is made up of micro-pipe and the filter cover being socketed on micro-pipe;
Automatic sampling apparatus, its can the sampling probe that is mounted thereon of three-dimensional running fix, this sampling probe can be loaded into the sample liquid fast transfer drawn in sample cell in the integrated Reagent Tube in project integrated card;
Project team's parallel reaction processes device, it includes a rotating disk driven by sequential stepping motor rotating shaft, rotating disk is distributed radially have and some is available for the draw-in groove that project integrated card laterally snaps in or takes out, rotating disk also rotate clockwise on direction sequentially interval arrange the measuring samples filter pressing to project integrated card provide source of the gas the first pressue device, project integrated card provides a liquid-adding device of reactant liquor, a pair reactant liquor filter pressing provide the second pressue device of source of the gas, one for the filter cover of integrated Reagent Tube being taken off the capping device abandoned;
Wash mill, washing head thereon is to being washed by micro-pipe in project integrated card below and waste liquid being extracted out micro-pipe;
Luminous substrate filling apparatus, liquid feeding thereon adds injection luminous substrate liquid for by micro-pipe in project integrated card below;
Photon detection counting assembly, the photomultiplier tube under it is to micro-pipe detection number of photons in the project integrated card of load luminous substrate liquid, and photon detection counting assembly can convert relative luminous intensity RLU numerical value to number of photons accumulative in the unit interval in micro-pipe.
The filter cover of described integrated Reagent Tube is fixed with a binding film from top to bottom that be made up of sample filter membrane and label film-stack. Determinand is obtained by reacting electrochemiluminescent immunoassay detection object reaction chain again by instilling the slight pipe of solid phase in advance after binding film primary response.
Described building floor choosing device is positioned at after stand, building floor choosing device includes the first column and lifts the first slide being socketed on the first column, first slide is installed first motor coordinating with the first column to drive the first slide to lift on the first column, a mechanism case is fixed in first slide side, mechanism case installs one by the motor-driven expansion link of second on mechanism case towards stand horizontal direction, and this expansion link is for releasing described pallet or drawing in stand. The first slide lifting is driven by the first motor, make mechanism case be elevated to expansion link and point to any one tray position on stand, drive expansion link to stretch out by the second motor and tray both slid is released before stand, make three-dimensional localization pickup apparatus integrated Reagent Tube can be picked up integrated project card from pallet inspection, constitute the detection project of a sufferer; Reflection drives the second motor, pallet can be withdrawn on stand by expansion link and reset.
Described three-dimensional localization pickup apparatus is positioned at before stand, three-dimensional localization pickup apparatus includes the second column and lifts the second slide being socketed on the second column, second slide is installed driving second slide the 3rd motor along the second column up-down, it is provided with first horizontal slide rail parallel with stand left and right in the second slide side, first horizontal slide rail is socketed the 3rd slide that can horizontally slip, 3rd slide is installed one and coordinates the 4th motor to drive the 3rd slide to slide on the first horizontal slide rail with the first horizontal slide rail, it is provided with second horizontal slide rail parallel with before and after stand in the 3rd slide side, second horizontal slide rail is socketed one the 4th slide, 4th slide is provided with and coordinates to drive the 4th slide slide anteroposterior the 5th motor with the second horizontal slide rail, one is fixed for the grappling fixture of the integrated Reagent Tube of pick-and-place below 4th slide. the second slide is driven to slide up and down along the second column by the 3rd motor, 4th motor drives the 3rd slide to horizontally slip along the first horizontal slide rail, 5th motor drives the 4th slide along the second horizontal slide rail slide anteroposterior, realize the grappling fixture running fix in the three dimensions in stand front being fixed on below the 4th slide, to make an inspection tour the integrated Reagent Tube that inspection picks out on the pallet of any one layer of release stand, and put in project integrated card to constitute a detection project being examined the some integrated Reagent Tube picked out by grappling fixture, the three-dimensional localization pickup apparatus of the present invention can be put into integrated Reagent Tube one by one in project integrated card continuously in this way, to constitute the detection project of one group of group.
Described automatic sampling apparatus, the first slide block on first crossbeam of it first crossbeam including level and sliding installation, the second cross beam of level and the sliding installation the second slide block on second cross beam, second cross beam is perpendicular to first crossbeam and affixed with the first slide block, described sampling probe is retractably installed at the second slider bottom, drive the first slide block along first crossbeam slide anteroposterior by the 6th motor being fixed on the first slide block, drive the second slide block to horizontally slip along second cross beam by the 7th motor being fixed on the second slide block; Second slide block side is fixed with the 8th motor for driving sampling probe to lift at the second slider bottom. 6th motor drives the first slide block slide anteroposterior on first crossbeam, second cross beam moves forward and backward with the first slide block, then passing through the 7th motor drives the second slide block to horizontally slip along second cross beam, the sampling probe making the second slider bottom can move in two-dimensional level face, then pass through the 8th motor and drive sampling probe lifting, sampling probe can be realized from moving to the arbitrarily the same organ pipe position of sample organ pipe dish, rise after drawing sample liquid by sampling probe, then moving in project integrated card above integrated Reagent Tube, decline sampling probe release sample liquid is in the filter cover of integrated Reagent Tube; Sample liquid can be loaded into continuously in this way to the integrated Reagent Tube of multiple project integrated cards.
Described capping device includes the fork of a L-shaped, the cross bar end of fork installs a pair by embracing motor-driven armful of folder of folder, the montant portion end of fork connects the rotation motor that a driving fork rotates, and a capping motor that fork can be driven to lift also is installed in the montant portion of the fork above rotation motor. when needing the filter cover of integrated Reagent Tube to separate with micro-pipe, the draw-in groove cross bar of fork being transferred in rotating disk by rotation motor is aligned above, then pass through capping motor and reduce fork height, make embrace folder alignment project integrated card in integrated Reagent Tube filter cover outside, an armful folder is driven to clamp filter cover again through embracing folder motor, capping motor drives fork to rise and filter cover is separated with micro-pipe, then pass through rotation motor and fork is screwed out rotating disk, armful filter cover that folder is moved out to outside rotating disk is made to abandon position, then passing through armful folder motor drives an armful folder to unclamp, filter cover is made to fall in the discard position of filter cover at self gravitation.
The 9th motor of a driving washing head lifting is installed in the side of described wash mill, and wash mill top is additionally provided with one and supplies the liquid inlet joint and that cleaning mixture enters washing head to discharge the drawing liquid joint of waste liquid for washing head suction; The feed liquor mode of liquid inlet joint is hydraulic pressure and the pulsed feed liquor of washing time interruption. When the integrated cartoon of project is crossed below wash mill, 9th motor drives washing head lifting to stretch in micro-pipe of integrated Reagent Tube one by one, then pass through liquid inlet joint and rinse micro-pipe to cleaning mixture with pulsed, and produce negative pressure by drawing liquid joint, and by cleaning mixture, waste liquid is extracted out, in this way each the micro-pipe in each project integrated card is carried out.
Described washing head has the outer tube communicated with liquid inlet joint and the interior pipe communicated with drawing liquid joint, and on outer tube, circumference has some spray holes for rinsing micro-inside pipe wall; Micro-pipe of integrated Reagent Tube is carried out surrounding and bottom irrigation by the spray hole on the outer tube of washing head, then by interior pipe, the waste liquid after flushing is pumped out micro-pipe.
Described luminous substrate filling apparatus top is additionally provided with the liquid feed tube joint of the luminous end communicated with liquid feeding pin. When each project integrated card is through luminous substrate filling apparatus, add substrate pin when the integrated reagent card of bottom promotion project team moves, one by one integrated reagent cup is added luminous substrate.
Described photon detection counting assembly is arranged on in the enterable darkroom of project integrated card. For avoiding the extraneous light impact on photon detection counting assembly, photon detection counting assembly is arranged in darkroom, when the integrated cartoon of project crosses darkroom, the photomultiplier tube of photon detection counting assembly is to micro-pipe detection number of photons in the project integrated card of load luminous substrate liquid, photon detection counting assembly can convert relative luminous intensity RLU numerical value to number of photons accumulative in the unit interval in micro-pipe, to indicate the immunoreactive reactant quantity of participation, by with the calibration object curve ratio stored relatively, drawing measured object concentration in sample.
Beneficial effects of the present invention is as follows:
1. the present invention adopts project team parallel reaction to process device, makes test event parallel running in the way of project integrated card, detection needed for arbitrary patient, fast report, greatly improves detection efficiency;
2. adopt intelligentized automatic sample, make each index of project team be able to concurrent testing, save loading time, ensure that the accuracy of application of sample simultaneously;
3. the filter pressing in rotating disk, washing adopt detection in place, Based Intelligent Control, automatically process each integrated reagent action required of project team, efficient, controlled, reliable, improve detection speed;
4. continuous pump drainage waste liquid when the present invention washs, pulsed injects washing liquid, and washing quickly, thoroughly, shortens wash time, improves the complete machine speed of service;
5. apparatus of the present invention coordinate each other with integrated reagent, can well washed corpuscles and for the instant detection of whole blood sample, be suitable for the samples such as whole blood, serum, blood plasma and other body fluid.
Accompanying drawing explanation
Fig. 1 is the block diagram (being distributed by automatic testing process) of complete machine of the present invention.
The integrated Reagent Tube that Fig. 2 is the present invention stores up the perspective view that fetching is put.
Fig. 3 is the structure sectional view of the integrated Reagent Tube of the present invention.
Fig. 4 is the perspective view of the automatic sampling apparatus of the present invention.
Fig. 5 is the perspective view of project team's parallel reaction process device of the present invention.
Fig. 6 is the perspective view of the capping device of the present invention.
Fig. 7 be micro-pipe of the integrated Reagent Tube of the present invention washing and add luminous substrate liquid structural representation.
Fig. 8 is the structural representation that the micro-pipe in project integrated card is carried out photon detection by the photon detection counting assembly in the darkroom of the present invention.
In figure, accompanying drawing is designated: 10. integrated Reagent Tube storage fetching is put; 11. stand; 111. pallet; 1110. position released by pallet; 12. building floor choosing device; 121. the first column; 122. the first slide; 123. the first motor; 124. mechanism case; 125. expansion link; 126. the second motor; 13. three-dimensional localization pickup apparatus; 131. the second column; 132. the second slide; 133. the 3rd motor; 134. the first horizontal slide rail; 135. the 3rd slide; 136. the 4th motor; 137. the second horizontal slide rail; 138. the 4th slide; 1381. grappling fixture; 139. the 5th motor; 14. project integrated card; 15. integrated Reagent Tube; 151. micro-pipe; 152. filter cover; 153. binding film; 154. sample filter membrane; 155. label film; 20. automatic sampling apparatus; 21. first crossbeam; 22. the first slide block; 23. the 6th motor; 24. second cross beam; 25. the second slide block; 26. the 7th motor; 27. sampling probe; 28. the 8th motor; 29. sample cell; 291. sample cell dish; 30. project team's parallel reaction processes device; 31. rotating shaft; 32. rotating disk; 33. draw-in groove; 34. the first pressue device; 35. liquid-adding device; 36. the second pressue device; 37. capping device; 371. rotation motor; 372. capping motor; 373. fork; 374. embrace folder; 375. embrace folder motor; 40. washing and interpolation substrate device; 41. wash mill; 411. washing head; 4111. outer tube; 4112. interior pipe; 4113. spray hole; 412. liquid inlet joint; 413. drawing liquid joint; 414. the 9th motor; 42. luminous substrate filling apparatus; 421. liquid feeding pin; 422. luminous end liquid feed tube joint; 50. photon detection counting assembly; 501. darkroom; 51. photomultiplier tube.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is further described.
As shown in Figure 1, the whole blood automatic luminous immunoassay instant detector of the present invention, it sequentially mainly includes, and integrated Reagent Tube storage fetching puts 10, automatic sampling apparatus 20, project team's parallel reaction process device 30, washing and add substrate device 40 and photon detection enumerator 50, as it is shown on figure 3, integrated Reagent Tube 15 used in the present invention is made up of micro-pipe 151 and the filter cover 152 being socketed on micro-pipe 151, filter cover 152 is fixed with a binding film 153 from top to bottom that be made up of sample filter membrane 154 and label film 155 stacking, the integrated Reagent Tube storage fetching of the present invention is put 10 and can be set up integrated multiple detection purpose project integrated card 14 by rapid batch, quickly each integrated Reagent Tube 15 in each project integrated card 14 is added sample liquid by automatic sampling apparatus 20, then pass through project team's parallel reaction process device 30 some groups of project integrated card 14 parallel reactions are processed, then pass through washing and add substrate device 40 to interpolation luminous substrate liquid after micro-pipe 151 washing of the integrated Reagent Tube 15 in projects integrated card 14, then by photon counting detector 50, the micro-pipe 151 in projects integrated card 14 is carried out photon detection to carry out luminescence immunoassay.
As depicted in figs. 1 and 2, the integrated Reagent Tube storage fetching of the present invention puts 10, including placing a stand 11 of some pallets 111 for stacking, for the pallet 111 of any one layer being released or drawn in the building floor choosing device 12 and of stand 11 for the some integrated Reagent Tube 15 in the pallet 111 releasing stand 11 positions the three-dimensional localization pickup apparatus 13 of taking-up. the building floor choosing device 12 of the present invention is positioned at after stand 11, building floor choosing device 12 includes the first column 121 and lifts the first slide 122 being socketed on the first column 121, first slide 122 is installed first motor 123 coordinating with the first column 121 to drive the first slide 122 to lift on the first column 121, a mechanism case 124 is fixed in first slide 122 side, mechanism case 124 installs an expansion link 125 driven by the second motor 126 on mechanism case 124 towards stand 11 horizontal direction, and this expansion link 125 is for releasing or draw in stand 11 by described pallet 111, the first slide 122 is driven to lift by the first motor 123, mechanism case 124 is made to be elevated to any one pallet 111 position that expansion link 125 points on stand 11, by the second motor 126 drive expansion link 125 stretch out pallet 111 slide release stand 11 before, making pallet 111 be in pallet and release position 1110, the integrated Reagent Tube 15 being positioned on the pallet 111 of pallet release position 1110 is available for three-dimensional localization pickup apparatus 13 inspection and picks up. the three-dimensional localization pickup apparatus 13 of the present invention is positioned at before stand 11, three-dimensional localization pickup apparatus 13 includes the second column 131 and lifts the second slide 132 being socketed on the second column 131, second slide 132 is installed the 3rd motor 133 that driving second slide 132 lifts along the second column 131, it is provided with first horizontal slide rail 134 parallel with stand about 11 in the second slide 132 side, first horizontal slide rail 134 is socketed the 3rd slide 135 that can horizontally slip, 3rd slide 135 is installed the 4th motor 136 coordinating with the first horizontal slide rail 134 to drive the 3rd slide 135 to slide on the first horizontal slide rail 134, it is provided with second horizontal slide rail 137 parallel with before and after stand 11 in the 3rd slide 135 side, second horizontal slide rail 137 is socketed one the 4th slide 138, 4th slide 138 is provided with and coordinates with the second horizontal slide rail 137 to drive the 4th slide 138 slide anteroposterior the 5th motor 139, one is fixed for the grappling fixture 1381 of the integrated Reagent Tube 15 of pick-and-place below 4th slide 138, 3rd motor 133 drives the second slide 132 to slide up and down along the second column 131, 4th motor 136 drives the 3rd slide 135 to horizontally slip along the first horizontal slide rail 134, 5th motor 139 drives the 4th slide 138 along the second horizontal slide rail 137 slide anteroposterior, realize grappling fixture 1381 running fix in the three dimensions in stand 11 front being fixed on below the 4th slide 138, to make an inspection tour the integrated Reagent Tube 15 examined on the pallet 111 picking out any one layer of release stand 11 front tray release position 1110, and examined the some integrated Reagent Tube 15 picked out and put in project integrated card 14 by grappling fixture 1381 to constitute a detection project, the three-dimensional localization pickup apparatus 13 of the present invention can be put into integrated Reagent Tube 15 one by one in project integrated card 14 continuously in this way, to constitute the detection project of one group of group. compared with putting with traditional Reagent Tube storage fetching, it is less that the integrated Reagent Tube storage fetching of the present invention puts 10 volumes, and the pallet 111 storing integrated Reagent Tube 15 is more, and three-dimensional localization pickup apparatus 13 is more flexible to picking and placeing of integrated Reagent Tube 15.
As shown in Figure 1 and Figure 4, the automatic sampling apparatus 20 of the present invention includes first crossbeam 21 and the sliding installation first slide block 22 on first crossbeam 21 of level, the second cross beam 24 of level and sliding installation the second slide block 25 on second cross beam 24, second cross beam 24 be perpendicular to first crossbeam 21 and with the first slide block 22 affixed 22, sampling probe 7 is retractably installed at bottom the second slide block 25, drive the first slide block 23 along first crossbeam 21 slide anteroposterior by the 6th motor 23 being fixed on the first slide block 22, the second slide block 25 is driven to horizontally slip along second cross beam 24 by the 7th motor 26 being fixed on the second slide block 25, second slide block 25 side is fixed with the 8th motor 28 for driving sampling probe 27 at the second slide block 25 bottom elevation, by the present invention can the sampling probe 21 that is mounted thereon of three-dimensional running fix, in the filter cover 152 of the integrated Reagent Tube 15 that this sampling probe 27 can be loaded in project integrated card 14 the sample liquid fast transfer drawn in the sample cell 29 on sample cell dish 291, in this way can continuous multiple project integrated cards 14 integrated Reagent Tube 15 filter cover 152 in be loaded into sample liquid.
As shown in Figure 1 and Figure 5, project team's parallel reaction of the present invention processes device 30, it includes a rotating disk 32 driven by sequential stepping motor rotating shaft 31, rotating disk 32 is distributed radially have and some is available for the draw-in groove 33 that project integrated card 14 laterally snaps in or takes out, also rotate clockwise on direction sequentially interval at rotating disk 32 to arrange the measuring samples filter pressing to project integrated card 14 first pressue device 34 of source of the gas is provided, project integrated card is provided a liquid-adding device 35 of 14 reactant liquors, a pair reactant liquor filter pressing provides the second pressue device 36 of source of the gas, one for taking off, the filter cover 152 of integrated Reagent Tube 15, the capping device 37 abandoned, treat that sliding project is by having added in the draw-in groove 33 that the project integrated card 14 of sample liquid snaps in rotating disk 32 by promoting device one by one a group by a group, projects integrated card 14 rotates clockwise along with rotating disk 32, rotating disk 32 is with sequential machine shaft 31 intermittent rotary, when project integrated card 14 moves to the first pressue device 34, filter cover 152 on Reagent Tube 15 integrated in project integrated card 14 is pressurizeed by the first pressue device 34, press by instilling bottom micro-pipe 151 after binding film 153 filter pressing under testing sample is passed through, when project integrated card 14 continues to turn to liquid-adding device 35 with rotating disk 32, liquid-adding device 35 injects reactant liquor to the filter cover 152 on integrated Reagent Tube 15, when the project integrated card 14 injecting reactant liquor moves to the second pressue device 36, second pressue device 36 reactant liquor filter pressing is washed the initial reactant on binding film 153 and instills bottom micro-pipe 151, solid formation on the micro-pipe 151 of solid phase in advance is combined, and sets up reaction chain. the integrated reagent card standing and reacting of project team of load sample is pulled to below capping device 37 to the time of regulation. as shown in Figure 5 and Figure 6, the capping device 37 of the present invention includes the fork 373 of a L-shaped, the cross bar end of fork 373 installs a pair by embracing armful folder 374 that folder motor 375 drives, the montant portion end of fork 373 connects the rotation motor 371 that a driving fork 373 rotates, and a capping motor 372 that fork 373 can be driven to lift also is installed in the montant portion of the fork 373 above rotation motor 371, the draw-in groove 33 being transferred in rotating disk 32 by the cross bar of 371 forks 373 of rotation motor is aligned above, then pass through capping motor 372 and reduce fork 373 height, make to embrace folder 374 and be directed in project integrated card 14 outside the filter cover 152 of integrated Reagent Tube 15, an armful folder 374 is driven to clamp filter cover 152 again through embracing folder motor 375, capping motor 372 drives fork 373 to rise and filter cover 152 is separated with micro-pipe 151, then pass through 371 forks 373 of rotation motor and screw out rotating disk 32, armful filter covers that folder 374 is moved out to outside rotating disk 32 are made to abandon position, then passing through armful folder motor 375 drives an armful folder 374 to unclamp, filter cover 152 is made to fall in the discard position of filter cover at self gravitation, so that the filter cover 152 on Reagent Tube 15 integrated in project integrated card 14 one by one is taken off. then the project integrated card 14 with micro-pipe 151 is released from draw-in groove 33, enter washing and add luminous substrate link.
As shown in Figure 1 and Figure 7, the present invention washing and add substrate device 40 be made up of wash mill 41 and luminous substrate filling apparatus 42; The 9th motor 414 of driving washing head 411 lifting is installed in the side of the wash mill 41 of the present invention, and wash mill 41 top is additionally provided with one and supplies the liquid inlet joint 412 and that cleaning mixture enters washing head 411 to discharge the drawing liquid joint 413 of waste liquid for washing head 411 suction; Washing head 411 has the outer tube 4111 communicated with liquid inlet joint 412 and the interior pipe 4112 communicated with drawing liquid joint 413, on outer tube 4111, circumference has some spray holes 4113 for rinsing micro-pipe 151 inwall, and the feed liquor mode of liquid inlet joint 412 is hydraulic pressure and the pulsed feed liquor of washing time interruption; By the movement of washing head 411, the micro-pipe 151 of each in project integrated card 14 is washed, micro-pipe 151 of integrated Reagent Tube 15 is carried out surrounding and bottom irrigation by the spray hole 4113 on the outer tube 4111 of washing head 411, and the waste liquid after then being rinsed by interior pipe 4112 pumps out micro-pipe 151; Micro-pipe 151 is sent to luminous substrate filling apparatus 42 position through the project integrated card 14 of washing. Luminous substrate filling apparatus 42 top of the present invention is additionally provided with a luminous substrate liquid feed tube joint 422 communicated with liquid feeding pin 421, luminous end liquid feed tube joint 422 by hose connection to luminous substrate liquid holding bottle, add injection luminous substrate liquid by the liquid feeding pin 421 on luminous substrate filling apparatus 42 to by each micro-pipe 151 in project integrated card 14 below, produce luminescence-producing reaction; Then the project integrated card 14 of load luminous substrate liquid is sent in the darkroom residing for photon detection enumerator 50.
As illustrated in figures 1 and 8, photomultiplier tube 51 under the photon detection counting assembly 50 of the present invention in the project integrated card 14 to load luminous substrate liquid micro-pipe 151 carry out number of photons detection, photon detection enumerator can convert relative luminous intensity RLU numerical value to number of photons accumulative in the unit interval in micro-pipe 151; RLU-concentration function is set up according to the calibration object curve stored in instrument controlling center, calculates on each project integrated card 14 each detection purpose concentration and provides examining report.
The electromechanical actuation mode of the present invention may be used without the modes such as V belt translation, screw rod transmission, minitype cylinder transmission.
Above example is used for illustrative purposes only, but not the present invention is limited, those skilled in the art, without departing from the spirit and scope of the present invention, various conversion or change can also be made, therefore, all equivalent technical schemes also should belong to scope of the invention, each claim limit.
Claims (10)
1. whole blood automatic luminous immunoassay instant detector, including photon detection enumerator (50), it is characterised in that it also sequentially includes:
Integrated Reagent Tube storage fetching puts (10), it includes placing a stand (11) of some pallets (111) for stacking, positioning, for the some integrated Reagent Tube (15) in the pallet (111) to release stand (11), the three-dimensional localization pickup apparatus (13) taken out for the building floor choosing device (12) and pallet (111) of any one layer being released or being drawn in stand (11), and the integrated Reagent Tube (15) that will be taken out from pallet (111) by three-dimensional localization pickup apparatus (13) loads in project integrated card (14); Described integrated Reagent Tube (15) is made up of micro-pipe (151) and the filter cover (152) being socketed on micro-pipe (151);
Automatic sampling apparatus (20), its can the sampling probe (21) that is mounted thereon of three-dimensional running fix, this sampling probe (27) can be loaded into the sample liquid fast transfer drawn in sample cell (29) in the integrated Reagent Tube (15) in project integrated card (14);
Project team's parallel reaction processes device (30), it includes a rotating disk (32) driven by sequential stepping motor rotating shaft (31), rotating disk (32) is distributed radially have and some is available for the draw-in groove (33) that project integrated card (14) laterally snaps in or takes out, also rotate clockwise on direction sequentially interval at rotating disk (32) to arrange the measuring samples filter pressing to project integrated card (14) first pressue device (34) of source of the gas is provided, project integrated card is provided a liquid-adding device (35) of (14) reactant liquor, a pair reactant liquor filter pressing provides second pressue device (36) of source of the gas, one for taking off, the filter cover (152) of integrated Reagent Tube (15), the capping device (37) abandoned,
Wash mill (41), washing head thereon (411) is to being washed by micro-pipe (151) in project integrated card (14) below and waste liquid being extracted out micro-pipe (151);
Luminous substrate filling apparatus (42), in the liquid feeding pin (421) the thereon project integrated card (14) to passing through below, micro-pipe (151) adds injection luminous substrate liquid;
Photon detection counting assembly (50), photomultiplier tube (51) under it is to micro-pipe (151) detection number of photons in the project integrated card (14) of load luminous substrate liquid, and photon detection enumerator can convert relative luminous intensity RLU numerical value to number of photons accumulative in the unit interval in micro-pipe (151).
2. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that: the filter cover (152) of described integrated Reagent Tube (15) is fixed with a binding film (153) from top to bottom that be made up of sample filter membrane (154) and label film (155) stacking.
3. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterized in that: described building floor choosing device (12) is positioned at after stand (11), the first slide (122) that building floor choosing device (12) includes the first column (121) and lifting is socketed on the first column (121), first slide (122) is upper installs first motor (123) coordinating to drive the first slide (122) in the upper lifting of the first column (121) with the first column (121), a mechanism case (124) is fixed in first slide (122) side, mechanism case (124) installs an expansion link (125) driven by the second motor (126) on mechanism case (124) towards stand (11) horizontal direction, this expansion link (125) is for releasing or draw in stand (11) by described pallet (111).
4. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterized in that: described three-dimensional localization pickup apparatus (13) is positioned at before stand (11), the second slide (132) that three-dimensional localization pickup apparatus (13) includes the second column (131) and lifting is socketed on the second column (131), second slide (132) is upper installs the 3rd motor (133) that a driving the second slide (132) lifts along the second column (131), first horizontal slide rail (134) parallel with stand (11) left and right it is provided with in the second slide (132) side, the 3rd slide (135) that the upper socket one of first horizontal slide rail (134) can horizontally slip, the upper installation of 3rd slide (135) one coordinates to drive the 3rd slide (135) at upper the 4th motor (136) slided of the first horizontal slide rail (134) with the first horizontal slide rail (134), second horizontal slide rail (137) parallel with before and after stand (11) it is provided with in the 3rd slide (135) side, second horizontal slide rail (137) upper socket one the 4th slide (138), 4th slide (138) is provided with and coordinates to drive the 4th slide (138) slide anteroposterior the 5th motor (139) with the second horizontal slide rail (137), 4th slide (138) lower section fixes one for the grappling fixture (1381) of the integrated Reagent Tube of pick-and-place (15).
5. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterized in that: described automatic sampling apparatus (20), it first crossbeam (21) including level and the sliding installation the first slide block (22) on first crossbeam (21), the second cross beam (24) of level and sliding installation be the second slide block (25) on (24) on second cross beam, second cross beam (24) is perpendicular to first crossbeam (21) and affixed with the first slide block (22), described sampling probe (27) is retractably installed at the second slide block (25) bottom, drive the first slide block (23) along first crossbeam (21) slide anteroposterior by the 6th motor (23) being fixed on the first slide block (22), the second slide block (25) is driven to horizontally slip along second cross beam (24) by the 7th motor (26) being fixed on the second slide block (25), second slide block (25) side is fixed with the 8th motor (28) and is used for driving sampling probe (27) at the second slide block (25) bottom elevation.
6. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterized in that: described capping device (37) includes the fork (373) of a L-shaped, the cross bar end of fork (373) installs a pair by embracing armful folder (374) that folder motor (375) drives, the montant portion end of fork (373) connects the rotation motor (371) that driving fork (373) rotates, and a capping motor (372) that fork (373) can be driven to lift also is installed in the montant portion of the fork (373) of rotation motor (371) top.
7. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterized in that: the 9th motor (414) that driving washing head (411) lifts is installed in the side of described wash mill (41), wash mill (41) top is additionally provided with one and supplies the liquid inlet joint (412) and that cleaning mixture enters washing head (411) to discharge the drawing liquid joint (413) of waste liquid for washing head (411) suction; The feed liquor mode of liquid inlet joint (412) is hydraulic pressure and the pulsed feed liquor of washing time interruption.
8. whole blood automatic luminous immunoassay instant detector as claimed in claim 7, it is characterized in that: described washing head (411) has the outer tube (4111) communicated with liquid inlet joint (412) and the interior pipe (4112) communicated with drawing liquid joint (413), the upper circumference of outer tube (4111) has some spray holes (4113) for rinsing micro-pipe (151) inwall.
9. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that: described luminous substrate filling apparatus (42) top is additionally provided with luminous end liquid feed tube joint (422) communicated with liquid feeding pin (421).
10. whole blood automatic luminous immunoassay instant detector as claimed in claim 1, it is characterised in that: described photon detection counting assembly (50) is arranged on in project integrated card (14) enterable darkroom (501).
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| CN113419073A (en) * | 2020-03-30 | 2021-09-21 | 厦门先明生物技术有限公司 | High-flux full-automatic micro-fluidic fluorescence immunoassay device |
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Effective date of registration: 20191118 Address after: Haicang District of Xiamen City, Fujian province 361000 Xinyang Street Weng Kok Road No. 289 building third unit 10 branch Patentee after: XIAMEN XIANMING BIOTECHNOLOGY CO., LTD. Address before: Siming District Yuqing road Xiamen City, Fujian province 361003 No. 33 building B room 1302 Patentee before: Xu Enliang |