CN106680523A - Linear propulsion and parallel processing reaction device for integrated reagent group - Google Patents

Abstract

The invention discloses a reaction device for linearly advancing parallel processing of an integrated reagent group, which comprises a support frame. Cooperating linear slides are arranged on both sides of the support frame. The linear slide is provided with several stations along the direction of the slide. The linear slide is equipped with The integrated card holding device of the linear synchronous pusher is used to hold the integrated reagent card. The linear synchronous pusher pushes the integrated reagent card forward and resets along the linear slide. Injection device, negative pressure reactor, negative pressure washing device and luminescence substrate liquid addition device, the integrated reagents preloaded in the integrated reagent card complete the sample addition, reaction, washing and luminescence liquid addition operations. The invention solves the problem of parallel processing and reaction of multi-indicator integrated reagent groups, greatly shortens the interval between detection and report results of two patient detection item groups, and improves the efficiency of immune detection.

Description

An integrated reagent group linear propulsion parallel processing reaction device

technical field

The invention discloses a linearly advancing parallel processing reaction device for an integrated reagent group, in particular a parallel processing reaction device for in vitro diagnosis, real-time detection of multi-indicator arbitrary combination item groups.

Background technique

In the real-time detection process, parallel analysis and simultaneous reporting of any combination of multi-index results have important practical significance. Multiple indicators can provide doctors with more and more accurate diagnostic information for doctors to make a correct diagnosis; any combination of multiple indicators can be analyzed in parallel to better meet the real-time detection needs of different patient conditions. In the actual detection process, each patient has a different disease, and the test items and quantity are different; the item group detected by each patient is regarded as a unit to integrate the item group detection, and there must be a device that can be synchronously processed in groups to achieve synchronization Completion of reactions, these simultaneous processes are important for immediate detection of results reported in parallel by project teams.

The Danish Radiometer AQT90 FLEX Immunoassay Analyzer, the BioMerieux Vidas of France's Mérieux, and the Japanese Mitsubishi Chemical PATHFAST are a typical type of multi-index real-time detection device for whole blood samples, which can realize multi-index parallel analysis and report results in a single field. This type of device prepackages other liquid components into an integrated structure for a single person, and adds reagents when there is no need for detection; when this type of equipment is detected, the test index reagents of a patient are put on the machine in groups to complete the test; but this type After the test of this group of indicators is completed, the instrument will start the test of other patient items. One item group test for each patient is generally 16-20 minutes. After the first patient sample enters the test, the waiting time for the second patient to be tested is too long, and the efficiency is low, which is not conducive to large sample processing when there are many samples in outpatient clinics. .

The Luminex system is a multiple assay reporting system. The system adopts flow cytometry cell resolution technology, fluorescence-encoded microsphere recognition technology, and immunofluorescence labeling reporting technology, so that multiple indicators can be detected and reported at the same time in one sampling, and more than 100 indicators can be theoretically achieved. However, due to the limitation of detection indicators for the same patient in clinical use, the compatibility between the immunoreactive substances of each indicator, and the complexity of the instrument structure, the Luminex system has not become a routine method for clinical testing. In addition, the samples used can only be serum or plasma, and whole blood samples cannot be used, which is not suitable for immediate detection.

Therefore, in the real-time detection scheme, it is still of great significance to study the limited multi-index automatic parallel analysis technology suitable for clinical needs.

Contents of the invention

Aiming at the deficiencies of the prior art, the present invention provides a linear advance parallel processing reaction device for an integrated reagent set, aiming at solving the problem of parallel processing and reaction of multi-indicator integrated reagent sets. The invention parallelizes and completes the reaction process of the integrated reagent reaction unit on the multi-indicator arbitrary item group of each patient, greatly shortens the interval between detection and report results of the detection item groups of two patients, and improves the efficiency of immune detection.

To achieve the above object, the present invention is achieved through the following technical solutions:

An integrated reagent group linear propulsion parallel processing reaction device, which includes a support frame, the two sides of the support frame are provided with matching linear slideways, the linear slideway is provided with a number of stations along the slideway direction, and the linear slideway is equipped with a linear synchronous The integrated card holding device of the pusher is used to hold the integrated reagent card, and the linear synchronous pusher pushes the integrated reagent card to move forward and reset along the linear slideway, and the sample filling device and the reaction liquid filling device are arranged in sequence along the linear slideway , negative pressure reactor, negative pressure washing device and luminescent substrate liquid adding device, the integrated reagent preloaded on the integrated reagent card completes the operations of sample addition, reaction, washing and luminescent liquid addition.

Further, at least one group of integrated reagent cards is operated in parallel on the linear slideway, and each integrated reagent card group is controlled by a linear synchronous propeller to move forward along the linear slideway synchronously, the integrated card clamping device holds the integrated reagent card, and the linear synchronous propeller pushes The integrated card holding device and the integrated reagent card advance one station as a whole, the integrated card holding device unclamps and places the integrated reagent card on the corresponding station on the linear slide, and the linear synchronous pusher and the integrated card holding device return to the previous position. Station, and then continue to the next wheel clamping, advancing, and reset actions.

Further, the linear synchronous pusher includes an integrated card clamping device and a propulsion motor, wherein the integrated card clamping device includes an integrated card clamping beam, a connecting rod structure and a clamping motor, the integrated card clamping beam is a pair, and each integrated card The clamping beam is set on the outside of the corresponding linear slideway. There are several clamping teeth on the integrated card clamping beam. The two integrated card clamping beams are connected with the clamping motor through the connecting rod structure to realize the clamping action. The integrated card clamping device and the pusher Motor connection for forward movement.

Further, the sample filling device includes a sampler bracket and a sampler, the sampler bracket is arranged on the support frame, the line slides on both sides are located in the sampler bracket, and the sampler is arranged on the beam of the sampler bracket And slide along the crossbeam, the sampler includes a sampler control structure and a connected sample head, the sampler control structure controls the sampler head to inject quantitative samples into each reagent cup on the integrated reagent card of the sampler station, and A number of optical sensors are installed on the beam of the sample holder to control the displacement of the sample dispenser to realize in-position detection and sample addition without missing or mistaken addition.

Further, the reaction liquid filling device includes a reaction liquid sampling support and a reaction liquid sampler, the reaction liquid sampling support is erected on the support frame, the linear slides on both sides are located in the reaction liquid sampling support, and the reaction liquid sampler is set On the crossbeam of the reaction liquid sampling bracket and slide along the crossbeam, the reaction liquid sampler includes a reaction liquid sampling control structure and a connected sampling head, and the reaction liquid sampling control structure controls the sampling head to add the reaction liquid Quantitative reaction solution is injected into each reagent cup on the integrated reagent card of the station, and a number of optical sensors are installed on the beam of the reaction solution sampling bracket to control the movement and stop of the reaction solution sampler to realize in-situ detection and sample addition without missing addition. Do not add by mistake.

Further, the negative pressure reactor includes a negative pressure reactor support, an upper pressure plate, a negative pressure control plate and a negative pressure chamber, a vacuum pump, and the upper pressure plate and the negative pressure control plate are connected to the reactor support and can move toward each other to clamp the reaction station. Each integrated reagent tube on the integrated reagent card, the negative pressure control board is equipped with a membrane envelope that matches each reagent cup. The membrane envelope is a top-opening opening structure. connect.

Further, the upper pressure plate of the negative pressure reactor is pressed against the upper edge of the cup body of the integrated reagent tube, and the negative pressure control plate moves upward in parallel, and the membrane envelope is opened and inserted at the mouth of the integrated reagent tube, so that the integrated reagent tube and the negative pressure The chambers are connected to form a negative pressure state. The negative pressure formed by the vacuum pump drives the sample and reaction solution in the integrated reagent tube to move downward to form a transmembrane reaction. The vacuum pump forms a negative pressure power with controllable flow and time.

Further, the negative pressure chamber communicates with the waste liquid bottle through the negative pressure guide tube, the top of the waste liquid bottle is connected with the buffer bottle through the air guide tube, and the buffer bottle is connected with the vacuum pump through the air guide tube.

Further, the negative pressure washing device includes a washer bracket, a lotion sampler, a membrane envelope, a negative pressure chamber and a vacuum pump, the washer bracket is arranged on a support frame, and several lotion samplers are fixed on the lotion On the beam of the instrument bracket, the membrane envelope is located under the lotion sampler, and the membrane envelope is matched with the integrated reagent tube. The membrane envelope is a top-opening opening structure, and each membrane envelope is connected to the negative pressure chamber and the vacuum pump through the negative pressure guide tube. , the injection head of the lotion sampler moves towards the membrane envelope, the injection head of the lotion sampler presses down on the upper edge of the integrated reagent cup of the washing station, and opens the outlet through the pressure sensor to discharge the lotion. The catheter tube of the reagent cup opens the membrane envelope, so that the integrated reagent cup communicates with the negative pressure chamber to form a negative pressure state, and the residue and washing waste liquid in the integrated reagent cup chamber are pumped into the waste liquid bottle outside the tube.

Further, the luminescent substrate liquid adding device includes a luminescent support, a luminescent liquid sampling controller and a sampling needle, the luminescent support is arranged on the support frame, and the luminescent liquid sampling controller is arranged on the beam of the luminescent support and can slide along the beam The luminescent liquid sampling controller is connected to the sampling needle for injecting quantitative luminescent substrate liquid into each integrated reagent cup on the integrated reagent card of the luminescent station.

Furthermore, the integrated reagent card is a plastic card structure formed by injection molding at one time. There are several holes in the middle of the card that match the integrated reagents. The two ends of the integrated reagent card have wedge-shaped structures that are concave inward for the clip teeth of the integrated card clamping beam. Hold and push the integrated card to move. The reagent cup of the integrated reagent is composed of a cup body and a catheter. There is a fixed dry marker film and a solid-phase film structure in the cavity of the cup body. There are antigens/antibodies on the solid-phase film. As the reaction formation area, the immobilized antigen/antibody on the membrane fiber combines with the target to be tested in the sample, and forms an immune reaction chain with the marker on the membrane of the marker, and the unreacted substance migrates downward through the membrane gap to the reagent out of the cup. The integrated reagent is intelligently integrated and loaded on the integrated card by the integrator, which constitutes an integrated reagent card that implements all (mostly so, if it is not completed once and then integrates another integrated reagent card) item group parallel detection of the patient's test items.

After the above technical scheme is adopted in the present invention, the integrated reagent group is advanced step by step according to the set time interval, and a group of integrated reagent cards, that is, the reagent item group is integrated by any item group and gradually pushed to the adding position of the luminescent substrate to complete the entire detection process The process, until the measurement of the luminescence value and the reporting of the results, enables the patient detection items to advance and complete the reaction in groups in parallel, so as to achieve high-efficiency group instant luminescence immunoassays and analysis.

The beneficial effects of the present invention are as follows:

① The multi-index detection items of each patient are processed and reacted in groups in parallel, and the efficiency of multi-index real-time detection of in vitro luminescence immunity is improved.

②It greatly shortens the response interval between the test item groups among each patient, greatly shortens the waiting time of patients, and facilitates the processing of large samples when there are many samples.

③ The present invention can realize the combined reaction of any item group with multiple indicators, greatly increase the scope of applicable detection items of the instrument, and ensure the needs of multi-indicator and multi-disease detection and diagnosis.

Description of drawings

Fig. 1 is a schematic diagram of an embodiment of the present invention.

Fig. 2 is a schematic diagram of a parallel processing reactor for an integrated reagent group of the present invention.

Fig. 3 is a schematic diagram of the device for parallel washing and adding of luminescent substrate solution for the integrated reagent group of the present invention.

Fig. 4 is a schematic diagram of the integrated reagent card and integrated reagents of the present invention.

detailed description

The present invention will be further described below in conjunction with accompanying drawing:

Embodiment: Please refer to Fig. 1 to Fig. 4, an integrated reagent group linear propulsion parallel processing reaction device, which includes a support frame 11, the two sides of the support frame 11 are provided with matching linear slides 12, and the linear slides 12 slide along the There are several stations in the direction of the track, as shown in Figure 1 A, B, C, D, E, F, G, H, I, J, K, M, N, the linear slide 12 is equipped with a linear synchronous propeller 2 The integrated card holding device is used to hold the integrated reagent card 3, and the linear synchronous pusher 2 pushes the integrated reagent card 3 to move forward and reset along the linear slideway 12, and the sample filling device 4, reaction solution adding device and Injection device 5, negative pressure reactor 6, negative pressure washing device 7 and luminescent substrate solution adding device 8, integrated reagent card 3 pre-loaded integrated reagents to complete sample addition, reaction, washing and luminescent solution addition operations.

As shown in Figure 1, there is at least one set of integrated reagent cards 3 working in parallel on the linear slideway 12, which can be 2, 3 or more sets of integrated reagent cards working in parallel, and each set of integrated reagent cards is controlled and synchronized by a linear synchronous propeller 2. Move forward along the linear slideway, the integrated card clamping device clamps the integrated reagent card 3, the linear synchronous pusher 2 pushes the integrated card clamping device and the integrated reagent card forward one station as a whole, the integrated card clamping device unclamps and Place the integrated reagent card on the corresponding station on the linear slideway, the linear synchronous pusher and the integrated card clamping device return to the previous station, and then continue the next wheel clamping, advancing and reset actions. The linear synchronous propeller 2 of the present invention includes an integrated card clamping device and a propulsion motor 21, wherein the integrated card clamping device includes an integrated card clamping beam 22, a connecting rod structure and a clamping motor 23, and the connecting rod structure includes a longitudinal beam 24, a transverse Rod 25, vertical rod 26 and motor connecting rod 27, the integrated card clamping beam 22 is a pair, each integrated card clamping beam is arranged on the outside of the corresponding linear slideway 12, and the integrated card clamping beam is provided with several clamping teeth 28 , the two integrated card clamping beams are connected to the clamping motor 23 through the connecting rod structure to realize the clamping action, and the longitudinal beam of the integrated card clamping device is connected to the advancing motor through the advancing motor connecting rod 29 to realize the forward movement.

As shown in Figure 1, the sample filling device 4 of the present invention includes a sampler bracket 40 and a sampler. The sampler 41 is arranged on the beam 41 of the sampler support and slides along the beam. The sampler includes a sampler control structure 42 and a connected sample head 43. The sampler control structure controls the sampler head to the sampler. Quantitative samples are injected into each reagent cup on the integrated reagent card, and a number of optical sensors are installed on the beam of the sampler bracket to control the shifting and stopping of the sampler to realize in-situ detection and sample addition, without missing or mistakenly adding.

As shown in Figure 1 and Figure 2, the reaction liquid filling device 5 includes a reaction liquid sampling support 50 and a reaction liquid sampling device. In the liquid sampling support, the reaction liquid sampler is arranged on the beam 51 of the reaction liquid sampling support and slides along the beam. The reaction liquid sampler includes a reaction liquid sampling control structure 52 and a connected sampling head 53 , the reaction solution sampling control structure 52 controls the injection head 53 to inject quantitative reaction solution into each reagent cup on the integrated reagent card of the reaction solution adding station, and the crossbeam 51 of the reaction solution sampling support 50 is provided with several optical sensors to control the reaction The liquid sampler moves and stops to realize in-situ detection and sample addition, without missing or mistaken addition. Negative pressure reactor 6 comprises negative pressure reactor support 61, upper pressure plate 62, negative pressure control plate 63 and negative pressure chamber 64, vacuum pump 65, and upper pressure plate 62 and negative pressure control plate 63 are connected on the reactor support 61 and can face each other. Each integrated reagent tube or cup on the integrated reagent card that moves and clamps the reaction station, and the negative pressure control board 63 is provided with a membrane envelope 68 that cooperates with each reagent cup. The membrane envelope 68 is a top-opening opening structure, and the membrane envelope 68 A plurality of membrane seals 681 are formed at the openings of each membrane, such as cross openings or Pozi openings, and each membrane envelope is connected with the negative pressure chamber 64 and the vacuum pump 65 through the negative pressure guide tube 640 . The upper edge strip 621 of the upper pressure plate of the negative pressure reactor presses against the upper edge of the cup body of the integrated reagent tube, and the negative pressure control plate 63 moves upward in parallel, and pushes away the film envelope at the mouth of the integrated reagent tube cup or the catheter tube. Insert the integrated reagent tube to communicate with the negative pressure chamber to form a negative pressure state. The negative pressure formed by the vacuum pump drives the sample and reaction solution in the integrated reagent tube to move downward to form a transmembrane reaction. The vacuum pump forms a negative pressure power with controllable flow and time. The negative pressure chamber 64 communicates with the waste liquid bottle 66 through the negative pressure guide tube 640 , the top of the waste liquid bottle is connected with the buffer bottle through the air guide tube, and the buffer bottle is connected with the vacuum pump 65 through the air guide tube.

As shown in Figures 1 and 3, the negative pressure washing device 7 includes a washer bracket 70, a lotion sampler 71, a membrane envelope 72, a negative pressure chamber 73 and a vacuum pump 74, and the washer bracket 70 is set on the support frame 11. On the top, there are several lotion samplers 71 fixed on the beam of the washer bracket. The membrane envelope 72 is located below the lotion injector 71. The membrane envelope is matched with the integrated reagent tube. The membrane envelope 72 is a top-opening type. Opening structure, the opening of the membrane envelope forms a plurality of membrane seals, such as cross openings or rice-shaped openings, each membrane envelope is connected with the negative pressure chamber 73 and the vacuum pump 74 through the negative pressure guide tube 730, and the lotion sampler 71 The sampling head and the membrane envelope 72 move towards each other, and the sampling head of the lotion sampler 71 presses down on the edge of the upper mouth of the integrated reagent cup of the washing station, and opens the outlet through the pressure sensor 701 to discharge the lotion. The liquid pipe pushes open the membrane envelope, so that the integrated reagent cup communicates with the negative pressure chamber to form a negative pressure state, and the residue and washing waste liquid in the integrated reagent cup chamber are pumped into the waste liquid bottle 75 outside the pipe, and the waste liquid bottle 75 is connected with the vacuum pump A buffer bottle 76 is also provided between them. The luminescent substrate solution adding device 8 includes a luminescent support 80, a luminescent liquid sampling controller 81 and a sampling needle 82. The luminescent support 80 is arranged on the support frame 11, and the luminescent liquid sampling controller is arranged on the beam of the luminescent support 80 and can Sliding along the crossbeam, the luminescent liquid sampling controller 81 is connected to the sampling needle 82 for injecting quantitative luminescent substrate liquid into each integrated reagent cup on the integrated reagent card of the luminescent station.

As shown in Figure 1 and Figure 4, the integrated reagent card 3 is a plastic card structure formed by injection molding at one time. There are several holes 31 in the middle of the card that match the integrated reagent 32. Groove 33 is used for the clamping teeth of the integrated card clamping beam to clamp and push the integrated card to move. The reagent cup for integrating reagents is composed of a cup body and a catheter. There are fixed dry marker films and solid-phase films in the cup cavity Structure, there are antigens/antibodies on the solid phase of the solid phase membrane, which is the reaction formation area, and the solid phase antigen/antibody on the membrane fiber combines with the target to be tested in the sample, and forms an immune reaction chain with the marker on the marker membrane. The reactant moves down through the membrane through the membrane gap and is discharged out of the reagent cup. The integrated reagent is intelligently integrated and loaded on the integrated card by the integrator, which constitutes an integrated reagent card that implements all (mostly so, if it is not completed once and then integrates another integrated reagent card) item group parallel detection of the patient's test items.

As shown in Figures 1 to 4, an integrated reagent group linearly advances parallel processing and reaction device, that is, an immunoassay multi-index integrated reagent group linearly advances parallel processing and reaction device. The description of the linear synchronous propeller is as follows. The linear synchronous propeller propels the motor It is composed of a linear synchronous propeller driven by the integrated reagent card clamping motor and a linear track. The linear track is located inside the linear synchronous propeller. Stations A, B, C, D, and E are set on the track for corresponding operations of the integrated reagent card. , F, G, H, I, J, K, M, N, cooperate with other structures here to complete the corresponding processing process respectively. The linear synchronous propeller is located at the lower part and outside of the linear track. The linear synchronous propeller propeller includes clamping, propulsion motors, connecting rods connected thereto, and integrated card clamping structure; the linear synchronous propeller propels multiple sets of integrated reagent cards to move synchronously to one station under the drive of the propulsion motor, and then resets. The integrated card clamping structure includes clamping motor, integrated card clamping motor, connecting rod, linear synchronous thruster longitudinal beam, linear synchronous thruster beam, linear synchronous thruster vertical rod, linear synchronous thruster integrated card clamping beam, linear synchronous thruster The synchro thruster stringers have integrated clip teeth. When working, the clamping teeth are driven by the clamping motor to clamp the grooves at both ends of the integrated reagent card, and the linear synchronous propeller is driven by the propulsion motor together with the linear synchronous propeller and the integrated reagent card. Place the integrated reagent card on the corresponding A-N station on the linear track. The clamping motor releases clamping, and the linear synchronous thruster returns to the upper position together with the linear synchronous thruster thruster and the integrated reagent card under the drive of the propulsion motor. action. In this way, each integrated reagent card completes all operations from station A to station N, and completes the reaction and corresponding processing. The sample filling device includes a sampler bracket fixed on the outer side of the clamping beam of the integrated card of the linear synchronous pusher, a sampler slideway (that is, a beam) that runs along the longitudinal direction of the integrated reagent card, and a fixed-point slideway along the slideway. The injector control structure and the injector injection head. When the integrated reagent card of the reagent project group enters the sampling station B, the instrument starts the sampling action. After the control structure of the sampler completes the sampling, the sampler head is aimed at the integrated reagent tube (integrated reagent cup) to be sampled. , inject a specified amount of sample into the integrated reagent tube. And inject a specified amount of samples into each integrated reagent sequentially along the slideway, until all the integrated reagents in the same integrated card have finished adding samples, and each reagent of the integrated reagent card enters the reaction state. The linear synchronous pusher pushes the integrated reagent card that has been added with samples to the next station C for reaction. The slideway (beam of the sampler support beam) is equipped with an optical sensor to control the movement of the sampler control structure, so as to realize in-position detection and sample addition without missing or mistakenly adding. The reaction liquid filling device includes a reaction liquid sampling bracket fixed on the outer side of the clamping beam of the integrated card of the linear synchronous propeller, a reaction liquid sampling slideway running horizontally along the longitudinal direction of the integrated reagent card, and a fixed-point sliding slideway along the slideway. The reaction liquid sampling control structure and the reaction liquid sampling head. When the integrated reagent card of the reagent project group enters the reaction solution adding station E, the instrument starts to add the reaction solution. After the reaction solution injection control structure completes the sampling, the reaction solution sampling head is aimed at the integrated reagent tube to be added (integrated Reagent cup), inject a specified amount of reaction solution into the integrated reagent tube, and inject a specified amount of reaction solution into each integrated reagent in sequence along the slideway until all integrated reagents are filled with reaction solution to wash the sample chamber and the integrated reagent tube. Marker film, reagents of the integrated reagent card continue to react. The linear synchronous propeller pushes the integrated reagent card that has been added with the reaction solution to the next station F for reaction. The slideway is equipped with an optical sensor to control the stop of the reaction solution sampling control structure, so as to realize in-position detection and addition of the reaction solution without missing or mistaken addition. The negative pressure reactor consists of a negative pressure reactor support, a negative pressure reactor drive motor, an upper platen support, an upper platen, edge strips, a negative pressure plate support, a negative pressure control board, a top-opening membrane envelope, and a top-opening membrane seal It consists of sheet, negative pressure guide tube, negative pressure chamber, negative pressure guide tube, waste liquid bottle, waste liquid outlet control valve, air guide tube, buffer bottle, negative pressure chamber, and vacuum pump. The integrated reagent card is propelled by the linear synchronous propeller and enters the station F, and the negative pressure reactor drive motor drives the upper pressure plate and the negative pressure control plate to move relative to each other. The upper pressure plate is pressed against the upper edge of the reaction integrated reagent cup, and the lower negative pressure control plate moves upward in parallel, and the film seal is opened on the reaction integrated reagent catheter, so that the lower part of the integrated reagent reaction tube enters a negative pressure state and drives the integrated reagent. The sample and reaction solution in the tube move downward to form a transmembrane reaction. Under the action of the vacuum pump, the negative pressure device forms negative pressure in each pipeline and bottle body, and is in a state of controllable flow rate and negative pressure time. Each integrated reagent card has undergone several negative pressure membrane penetration reactions until all samples have achieved membrane penetration reaction to ensure the accuracy of the test results to the greatest extent. The negative pressure washing device consists of a washer bracket, a lotion injection head fixing frame, a lotion filling control structure, a lotion injection head, a pressure sensor, a top-opening membrane envelope and a membrane sealing piece drive motor, and a negative pressure guide. Tube, negative pressure chamber, waste liquid bottle, waste liquid outlet control valve, air guide tube, buffer bottle, negative pressure chamber, and vacuum pump. When the integrated reagent card enters the M station, the lotion injection head, the top-opening membrane envelope and the membrane sealing piece move towards each other. The washing liquid injection head presses the upper edge of the integrated reagent tube, and opens the washing liquid head through the pressure sensor to discharge the washing liquid. The integrated reagent diversion tube tops off the membrane seal, so that the lower part of the integrated reagent reaction tube enters a negative pressure state, and the residues and washing waste liquid in the integrated reagent tube cavity are pumped out of the tube. Rinse, pump, and discharge in this way, and fully wash until the specified time is reached. The luminescent substrate liquid adding device is composed of a luminescent substrate liquid adding structural support, a luminescent substrate liquid sampling controller, and a luminescent substrate liquid sampling needle. When the integrated reagent card enters the N position, the instrument starts the luminescent substrate liquid sampling controller, and the luminescent substrate liquid injection needle sequentially adds a specified amount of luminescent substrate into each integrated reagent reaction tube along the luminescent substrate liquid sampling slideway. solution until all the luminescent substrate solutions are added. The integrated reagent reaction tubes added with the luminescent substrate solution are then transferred to the luminescent darkroom by other institutions, and the relative luminous intensity of each integrated reagent tube is measured one by one, the content of the target is calculated, and the corresponding target concentration and determination results of each integrated reagent are reported in parallel.

As shown in Figures 1 to 4, an integrated reagent group linearly advances parallel processing and reaction device, that is, an immunoassay multi-index integrated reagent group linearly advances parallel processing and reaction device, and an immune detection integrated reagent group linearly advances parallel processing and reaction device The device consists of a linear synchronous thruster and track, a sample filling device, a reaction liquid filling device and a negative pressure reactor, a negative pressure washing device, a luminescent substrate adding structure, an integrated reagent card and an integrated reagent, etc., to realize self-injection of samples Up to washing, adding luminescent substrates and processing in parallel, and reporting the results at the same time.

As shown in Figure 1 and Figure 4, the integrated card is the integrated card integrated reagent loading position or cavity 31 of the integrated reagent card 3, and the integrated reagent 32 is loaded into the station A of the linear slideway 12 on the fixed bracket (support frame 11). . The integrated card clamping motor 21 clamps the motor connecting rod 29, the linear synchronous propeller longitudinal beam 24, the linear synchronous propeller cross bar 25, the linear synchronous propeller vertical rod 26, and the linear synchronous propeller integrated card clamping beam 22 through the integrated card clamping motor link 29 , drive the linear synchronous propeller longitudinal beam integrated card clip teeth 28 to clamp the integrated reagent card 3 grooves loaded with integrated reagents, and drive the linear synchronous propeller propulsion motor 21 and the linear synchronous propeller propulsion motor connecting rod linkage action along the linear track , move from station A to station B for sample addition to implement sample injection.

As shown in Figure 1, the sampler control structure 42 on the sampler support 40 advances along the sampler slideway 41 to the integrated reagent position of the integrated reagent card to be sampled, and the set amount is added by the sampler injection head 43. samples into integrated reagent tubes. After completing the injection of an integrated reagent tube, inject a specified amount of sample into each integrated reagent sequentially along the slide until all the integrated reagents in the same integrated reagent card are added, and each reagent of the integrated reagent card enters the reaction state. The slideway is equipped with an optical sensor to control the stop of the sample injector control structure, so as to realize in-position detection and sample addition without missing or mistakenly adding. The linear synchronous pusher then advances the integrated reagent cards that have been added to the C, D, and E stations for reaction.

As shown in Figure 1, when the integrated reagent card is in the E station, the reaction solution sampling control structure 52 on the reaction solution sampling support 50 advances along the reaction solution sampling slideway 51 to the integrated reagent card integrated reagent of the reaction solution to be added. position, add a set amount of reaction solution to the integrated reagent tube from the reaction solution injection head 53. After adding the reaction solution to one integrated reagent tube, inject a specified amount of reaction solution into each integrated reagent sequentially along the slideway until all the integrated reagents in the same integrated reagent card complete the addition of the reaction solution, and the reagents of the integrated reagent card continue to react. The slideway is equipped with an optical sensor to control the stop of the reaction liquid sampler control structure, so as to realize on-site detection and addition of the reaction liquid without missing or mistaken addition. The linear synchronous propeller pushes the integrated reagent card that has been added with the reaction solution to the F station for reaction.

As shown in Figure 1, when the integrated reagent card is at the F position, the negative pressure reactor drive motor 601 on the negative pressure reactor support 61 drives the edge holder 621 on the upper platen 62 via the upper platen support 602 to press on the reaction plate. The upper edge of the integrated reagent cup body 603, and the negative pressure reactor driving motor 601 drives the negative pressure control plate 63 to move upward through the negative pressure plate support 604, and the integrated reagent catheter 6030 contacts with the top-opening membrane envelope 64 during the reaction, The open-top membrane sealing piece 641 is connected to the negative pressure chamber 64 through the negative pressure guide tube 640, and the integrated reagent card has an integrated reagent position to enter the negative pressure suction filtration reaction state. Under the action of the negative pressure formed by the vacuum pump 65, through the negative pressure chamber 64, the air guide tube 660 and the buffer bottle 67, the waste gas and waste liquid are guided and discharged to the waste liquid bottle 66 by the negative pressure guide tube 640, thus completing the negative pressure. transmembrane reaction. The F-K position of the linear track realizes the negative pressure membrane penetration reaction process. The linear synchronous pusher then pushes the integrated reagent card that has completed the negative pressure membrane penetration reaction to the M station for washing.

As shown in Figure 1, when the integrated reagent card is at the M position, the lotion injection head fixing frame (beam) on the washer bracket 70, the lotion injection heads of each lotion injector 71 are in the wash Under the control of the liquid filling control structure and the action of the pressure sensor 701, the upper edge of the integrated reagent is pressed down, the liquid injection function is turned on, and the washing liquid is added to the position with the integrated reagent. At the same time, the top-opening membrane envelope 72 and the membrane sealing piece drive the motor to move upward, the integrated reagent catheter contacts the top-opening membrane envelope 72, and the top-opening top-opening membrane sealing piece is connected by the negative pressure guide tube 730 Negative pressure chamber 73, the integrated reagent card has an integrated reagent position and enters the state of negative pressure suction filtration and washing. Under the action of the vacuum formed by the operation of the vacuum pump 74, through the negative pressure chamber 73, the air guide tube 750 and the buffer bottle 76, the waste gas and waste liquid are guided and discharged to the waste liquid bottle 75 by the negative pressure guide tube 730, thus completing the negative pressure washing . The linear synchronous propeller then pushes the integrated reagent card that has completed the negative pressure membrane penetration reaction to the N station to add the luminescent substrate solution.

As shown in Figure 1, when the integrated reagent card is at the N position, the luminescent substrate liquid sampling controller 81 on the luminescent substrate liquid adding structure support 80 advances along the luminescent substrate liquid sampling slideway (beam) to the waiting position. Add the luminescence substrate solution integrated reagent card integrated reagent position, add a set amount of luminescence substrate solution to the integrated reagent tube from the luminescence substrate solution injection needle 82 . After adding the luminescent substrate solution to one integrated reagent tube, inject a specified amount of luminescent substrate solution into each integrated reagent sequentially along the slideway until all the integrated reagents in the same integrated reagent card have finished adding the luminescent substrate solution, and each integrated reagent card The reagent enters a luminescent reaction state. The integrated reagent reaction tubes added with luminescent substrate solution are then transferred to the luminescence measurement darkroom by other institutions, and the relative luminous intensity of each integrated reagent tube is measured one by one, the content of the target is calculated, and the concentration of the corresponding target and the determination result of each integrated reagent are reported in parallel, and the completion An assay run with an integrated reagent card.

The above records are only examples of using the technical content of this creation. Any modifications and changes made by those who are familiar with this technology using this creation belong to the scope of patents claimed by this creation, and are not limited to those disclosed in the examples.

Claims (10)

1. A linear advance parallel processing reaction device for an integrated reagent group, characterized in that: it includes a support frame, the two sides of the support frame are provided with matching linear slideways, and the linear slideway is provided with several stations along the direction of the slideway, and the linear slideway The integrated card holding device equipped with a linear synchronous pusher is used to hold the integrated reagent card. The linear synchronous pusher pushes the integrated reagent card to move forward and reset along the linear slide. Along the linear slide, there are sample filling devices, Reaction liquid filling device, negative pressure reactor, negative pressure washing device and luminescent substrate liquid adding device, the integrated reagents preloaded in the integrated reagent card complete the sample adding, reaction, washing and luminescent liquid adding operations. 2. A linear propulsion parallel processing reaction device for integrated reagent groups according to claim 1, characterized in that: there is at least one group of integrated reagent cards working in parallel on the linear slide, and each integrated reagent card group is controlled by a linear synchronous propeller to synchronize along the line. The slideway moves forward, the integrated card clamping device clamps the integrated reagent card, the linear synchronous pusher pushes the integrated card clamping device and the integrated reagent card forward a station as a whole, the integrated card clamping device releases the clamping and integrates the reagent card The card is placed on the corresponding station on the linear slideway, and the linear synchronous pusher and the integrated card clamping device return to the previous station, and then continue the next wheel clamping, advancing and reset actions. 3. An integrated reagent group linear propulsion parallel processing reaction device according to claim 1, characterized in that: the linear synchronous propeller includes an integrated card holding device and a propulsion motor, wherein the integrated card holding device includes an integrated card holding device Beam, connecting rod structure and clamping motor. The integrated card clamping beam is a pair. Each integrated card clamping beam is set on the outside of the corresponding linear slideway. There are several clamping teeth on the integrated card clamping beam. Two integrated card clamps The holding beam is connected with the clamping motor through the connecting rod structure to realize the clamping action, and the integrated card clamping device is connected with the propulsion motor to realize the forward movement. 4. An integrated reagent group linear propulsion parallel processing reaction device according to claim 1, characterized in that: the sample filling device includes a sample injector bracket and a sample injector, the sample injector bracket is set on the support frame, and the two The side line slideway is located in the sample injector bracket, the sample injector is arranged on the beam of the sample injector bracket and slides along the beam, the sample injector includes a sample adding control structure and a connected sampling head, the sample adding control structure The injection head is controlled to inject quantitative samples into each reagent cup on the integrated reagent card of the sampling station, and a number of optical sensors are installed on the beam of the sampler bracket to control the displacement of the sampler to realize in-position detection and sample addition. 5. An integrated reagent group linear propulsion parallel processing reaction device according to claim 1, characterized in that: the reaction liquid filling device includes a reaction liquid sampling support and a reaction liquid sampling device, and the reaction liquid sampling support is erected on On the support frame, the linear slides on both sides are located in the reaction liquid sampling bracket. The reaction liquid sampler is arranged on the beam of the reaction liquid sampling bracket and slides along the beam. The reaction liquid sampler includes a reaction liquid sampling control The structure and the connected sampling head, the reaction liquid sampling control structure controls the sampling head to inject quantitative reaction liquid into each reagent cup on the integrated reagent card of the reaction liquid adding station. The optical sensor controls the movement and stop of the reaction liquid sampler to realize in-position detection and addition of reaction liquid. 6. An integrated reagent group linear propulsion parallel processing reaction device according to claim 1, characterized in that the negative pressure reactor includes a negative pressure reactor bracket, an upper pressure plate, a negative pressure control plate, a negative pressure chamber, and a vacuum pump, The upper pressure plate and the negative pressure control plate are connected to the reactor support and can move towards each other to clamp the integrated reagent tubes on the integrated reagent card of the reaction station. The envelope is a top-opening opening structure, and each membrane envelope is connected with the negative pressure cavity and the vacuum pump through the negative pressure guide tube. 7. An integrated reagent group linear propulsion parallel processing reaction device according to claim 1, characterized in that: the upper pressure plate of the negative pressure reactor is pressed against the upper edge of the cup body of the integrated reagent tube, and the negative pressure control plate is parallel to the upward direction. Move, open the membrane envelope at the mouth of the integrated reagent tube and insert it, so that the integrated reagent tube communicates with the negative pressure chamber to form a negative pressure state, and the negative pressure formed by the vacuum pump drives the sample and reaction solution in the integrated reagent tube to move downward to form a transmembrane reaction , the vacuum pump forms a negative pressure power with controllable flow and time, the negative pressure chamber is connected to the waste liquid bottle through the negative pressure guide tube, the top of the waste liquid bottle is connected to the buffer bottle through the air guide tube, and the buffer bottle is connected to the vacuum pump through the air guide tube. 8. An integrated reagent group linear propulsion parallel processing reaction device according to claim 1, characterized in that the negative pressure washing device includes a washer bracket, a lotion sampler, a membrane envelope, a negative pressure chamber and a vacuum pump, The bracket of the washer is set on the support frame. There are several lotion samplers fixed on the beam of the bracket of the washer. The membrane envelope is located under the lotion sampler. The membrane envelope is matched with the integrated reagent tube. The top-opening opening structure, each membrane envelope is connected with the negative pressure chamber and the vacuum pump through the negative pressure guide tube, the injection head of the lotion sampler and the membrane envelope move towards each other, and the injection head of the lotion sampler presses down The upper edge of the integrated reagent cup at the washing station is opened, and the outlet is discharged through the pressure sensor. The catheter tube of the reagent cup pushes open the membrane envelope, so that the integrated reagent cup communicates with the negative pressure chamber to form a negative pressure state, and the integrated reagent The residue in the cup cavity and the washing waste liquid are pumped into the waste liquid bottle outside the tube. 9. An integrated reagent group linear propulsion parallel processing reaction device according to claim 1, characterized in that: the device for adding luminescent substrate liquid includes a luminescent support, a luminescent liquid sampling controller and a sampling needle, and the luminescent support is set at On the support frame, the luminescent liquid sampling controller is set on the beam of the luminescent bracket and can slide along the beam. The luminescent liquid sampling controller is connected to the injection needle for injection into each integrated reagent cup on the integrated reagent card of the luminescent station. Quantitative luminescent substrate solution. 10. An integrated reagent group linear propulsion parallel processing reaction device according to any one of claims 1 to 9, characterized in that the integrated reagent card is a plastic card structure that is injection molded at one time, and there are several integrated reagent cards in the middle of the card. Matching holes, both ends of the integrated reagent card have concave wedge-shaped structures for the clamping teeth of the integrated card clamping beam to clamp and push the integrated card to move. The reagent cup of the integrated reagent is composed of a cup body and a catheter. There are fixed dry-type marker membranes and solid-phase membrane structures in the body cavity. The solid-phase membrane has antigens/antibodies on the solid-phase membrane, which is the reaction formation area. The immobilized antigens/antibodies on the membrane fibers bind to the target to be measured in the sample. And form an immune reaction chain with the marker on the marker membrane, and the unreacted substances move down through the membrane gap and discharge out of the reagent cup.