CN113567697A - Full-automatic chemiluminescence immunoassay analyzer - Google Patents

Full-automatic chemiluminescence immunoassay analyzer Download PDF

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Publication number
CN113567697A
CN113567697A CN202110845728.4A CN202110845728A CN113567697A CN 113567697 A CN113567697 A CN 113567697A CN 202110845728 A CN202110845728 A CN 202110845728A CN 113567697 A CN113567697 A CN 113567697A
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China
Prior art keywords
module
driving mechanism
reagent
sample
magnetic rod
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CN202110845728.4A
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Chinese (zh)
Inventor
唐金鑫
林�源
李蒙蒙
夏星
蒲建文
漆海林
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Sichuan Xinjian Kangcheng Biological Co ltd
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Sichuan Xinjian Kangcheng Biological Co ltd
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Priority to CN202110845728.4A priority Critical patent/CN113567697A/en
Publication of CN113567697A publication Critical patent/CN113567697A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/15—Preventing contamination of the components of the optical system or obstruction of the light path
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002—Reagent dispensers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004—Cleaning sample transfer devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices
    • G01N35/1074—Multiple transfer devices arranged in a two-dimensional array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G01N2035/00376—Conductive heating, e.g. heated plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00524—Mixing by agitating sample carrier
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G01N2035/00742—Type of codes
    • G01N2035/00752—Type of codes bar codes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N2035/1025—Fluid level sensing

Abstract

The invention discloses a full-automatic chemiluminescence immunoassay analyzer, wherein a rack is provided with a data processing and control module which is simultaneously connected with a sample storage module, a reagent cleaning and separating module, a reagent reaction module, a sample adding and transporting module, a lighting module and a suction head storage module, the suction head storage module is arranged between the sample storage module and the reagent reaction module, the reagent cleaning and separating module is arranged between the sample adding and transporting module and the lighting module, and the sample adding and transporting module is arranged between the reagent reaction module and the reagent cleaning and separating module. The invention can directly detect whole blood or serum samples, can fully automatically complete the operations of code scanning, sample adding and the like of the samples only by placing the samples on the sample rack after the preparation of consumables and related reagents is completed, finally outputs detection results, has high accuracy and stability of the detection results, has the cost obviously lower than that of a full-automatic chemiluminescence immunoassay analyzer on the market, and is convenient for large-scale popularization in the later period.

Description

Full-automatic chemiluminescence immunoassay analyzer
Technical Field
The invention belongs to the technical field of biochemical detection, and particularly relates to a full-automatic chemiluminescence immunoassay analyzer.
Background
The development of the chemiluminescence immunity technology which is started in the last 70 th century becomes a mature and advanced technology for detecting ultramicro active substances, and the application range is wide. The detection technology has the advantages of high sensitivity, strong specificity, low reagent price, stable reagent, long effective period (6-18 months), stable and rapid method, wide detection range, simple operation, high automation degree and the like. The existing market generally has a large chemiluminescent immunoassay analyzer packaged by a plurality of reagents, the analyzer occupies a large space and has high requirements on the sample amount, the reagents must be used within a specified time after being unsealed, and otherwise, the test result is inaccurate. This type of instrument is not suitable for hospitals with small sample volumes or for small town hospitals. Meanwhile, such large-scale instruments are complex to operate, operators can operate the instruments only through systematic training, and the instruments are not beneficial to popularization in small and medium hospitals with limited medical resources. Moreover, the cost of the large-scale instruments is very high, and the large-scale instruments are not borne by hospitals in villages and towns, and are not beneficial to the vigorous popularization of primary medical treatment. And there are less single reagent strip chemiluminescence immunoassay appearance in the existing market, and this kind of instrument needs artifical manual work to go to accomplish the application of sample process, has complex operation, test result because of the different inconsistent circumstances of operator, is unfavorable for a large amount of popularization.
Therefore, the full-automatic chemiluminescence immunoassay analyzer which is used for single-person reagent strip detection and has the advantages of low cost, small occupied area and convenient operation is very suitable for the market demand.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a full-automatic chemiluminescence immunoassay analyzer aiming at the defects of the prior art, wherein the analyzer has the advantages of small volume, complete functions, intelligent operation realization, high detection precision and small workload.
In recent years, due to the influence of factors such as environment on human body, new onset of infectious diseases has caused a sudden and constant threat to human health, and spread of infectious diseases such as atypical pneumonia, middle east respiratory syndrome, influenza, ebola, zika virus, yellow fever, rift valley fever and the like has become a global public health problem. With the development of urbanization and the increase of global trade, the spread risk of natural epidemic infectious diseases is aggravated by environmental changes, and the prevention and control of infectious diseases are not slow. Therefore, the method for carrying out instant and rapid detection of infectious diseases has great significance for the prevention and control work of infectious diseases.
With the development of society, accurate detection of different diseases and infectious diseases is important. Chemiluminescence is one of the most accurate and mature detection methods of recognized tumor markers and various hormones, the sensitivity and the accuracy of the chemiluminescence are higher than those of an enzyme immunoassay method and a fluorescence method by several orders of magnitude, and the detection result is more authoritative compared with the results obtained by the enzyme immunoassay method and the fluorescence method.
The chemiluminescence immunoassay technology has the advantages of high sensitivity, strong specificity, low reagent price, stable reagent, long effective period (6-18 months), stable and rapid method, wide detection range, simple operation, high automation degree and the like. In recent years, the technique has been rapidly developed as an in vitro diagnostic technique, and is applied to test items of a plurality of disciplines. The device can quickly obtain the detection result, is convenient for patients and reduces the time for the patients to confirm the diagnosis. Meanwhile, the chemiluminescence immune technology product has the function of continuously using to monitor the disease condition, for example, a patient with heart disease can quickly know and control the disease condition through detection of interval time. The market generally has a large chemiluminescent immunoassay analyzer packaged by a plurality of reagents, the analyzer occupies large space and has high requirements on sample amount, the reagents must be used within a specified time after being unsealed, and otherwise, the test result is inaccurate. This type of instrument is not suitable for hospitals with small sample volumes or for small town hospitals. Meanwhile, such large-scale instruments are complex to operate, operators can operate the instruments only through systematic training, and the instruments are not beneficial to popularization in small and medium hospitals with limited medical resources. Moreover, the cost of the large-scale instruments is very high, and the large-scale instruments are not borne by hospitals in villages and towns, and are not beneficial to the vigorous popularization of primary medical treatment. And there are less single reagent strip chemiluminescence immunoassay appearance in the existing market, and this kind of instrument needs artifical manual work to go to accomplish the application of sample process, has complex operation, test result because of the different inconsistent circumstances of operator, is unfavorable for a large amount of popularization.
Some other detection means in the market have some defects, for example, the radioimmunoassay with high detection sensitivity has problems of radioactive protection and isotope pollution, and moreover, the reagent is expensive and is difficult to popularize in the primary medical institution.
Chemiluminescence immunoassay can be divided into electrochemical luminescence immunoassay and chemiluminescence immunoassay according to the type and luminescence characteristics of a chemiluminescence substance, wherein chemiluminescence immunoassay can be divided into direct chemiluminescence immunoassay, enzymatic chemiluminescence immunoassay and luminol oxygen pathway immunoassay according to the difference of a luminescent agent. The scheme is based on the principle of magnetic particle enzymatic chemiluminescence immunoassay, adopts paramagnetic particle separation and other modes to realize the separation of free markers and immune compound markers, adopts a PMT single photon counting module to read luminescence signals, has good specificity and high sensitivity, and is a mainstream methodology accepted in clinic.
The technical scheme adopted by the invention is as follows: full-automatic chemiluminescence immunoassay appearance, which comprises a frame, it deposits the module to be provided with the sample in the frame, reagent washs separation module, reagent reaction module, application of sample transportation module, lighting module, module and data processing and control module are deposited to the suction head, wherein the sample is deposited the module, reagent washs separation module, reagent reaction module, application of sample transportation module, lighting module and suction head are deposited the module and are all connected with data processing and control module simultaneously, the suction head is deposited the module and is set up and deposit between module and reagent reaction module at the sample, reagent washs separation module and sets up between application of sample transportation module and lighting module, application of sample transportation module sets up between reagent reaction module and reagent washing separation module. The sample and corresponding single-person reagent strips, consumables and the like are put into the sample storage module, the sucker storage module and the reagent reaction module, and the sample test is automatically executed through operation. The process includes bar code scanning of the sample and reagent, and then the full-automatic mechanical arm adds the sample to the corresponding reagent strip for reaction by using a tip head. The full reaction of sample in the reagent strip is after several times washs, detects its luminous value, and data output testing result after software processing finally realizes full-automatic single sample detection, realizes intelligent operation, and the work load is little, and the detection precision is high simultaneously, through integrated structure, multiple functional, and small, not restricted to hospital central laboratory and use, can be used to departments such as clinical laboratory, outpatient service, ICU, intracardiac, uropoiesis, clinic, suitable for the object extensively.
Furthermore, the sample storage module comprises a first driving mechanism, the first driving mechanism is installed on the rack, the first driving mechanism is connected with a circulating device, the circulating device can realize circulating motion under the action of the first driving mechanism, a plurality of test tube racks are installed on the outer wall of the circulating device, and the test tube racks can move along with the circulating device; a yard device is swept to the circulating device outside being provided with, sweeps a yard device and can sweep the sign indicating number to the test tube in the test-tube rack, actuating mechanism one with sweep a yard device and all be connected with data processing and control module. The sample is deposited the module main function and is carried out depositing of sample, sweeps a yard test tube access information, sends different test tubes to same position and inhales the appearance through setting up opto-coupler accurate positioning test tube position.
Further, the suction head storage module comprises a driving mechanism II and a suction head storage support frame, the driving mechanism II and the suction head storage support frame are both installed on the rack, a first optical axis is arranged on the suction head storage support frame, a first sliding block is sleeved on the first optical axis and can move along the axis of the first optical axis, a suction head storage device is arranged on the first sliding block, the driving mechanism II is connected with a second conveying belt, the second conveying belt is connected with the first sliding block, the second driving mechanism can drive the second conveying belt to move so that the first sliding block can move back and forth, and the second driving mechanism is connected with the data processing and control module. The suction head storage module is mainly used for storing the suction heads and matching with the sample adding and transporting module to move to complete suction head supply.
Furthermore, the sample adding transportation module comprises a sample adding transportation supporting mechanism, the sample adding transportation supporting mechanism is installed on the rack, a sample adding device is arranged on the sample adding transportation supporting mechanism, the sample adding device can horizontally move along the sample adding transportation supporting mechanism, the sample adding device can vertically move along the axis of the sample adding device, and the sample adding device is connected with the data processing and control module. The sample adding transportation supporting mechanism comprises a transverse guide rail and a transverse driving mechanism, the transverse guide rail is fixed with the sample adding transportation supporting mechanism, a plurality of sample adding belt wheels are arranged on the sample adding transportation supporting mechanism, a transverse synchronous belt is connected to the transverse driving mechanism, the transverse synchronous belt is simultaneously connected with the sample adding belt wheels, the transverse synchronous belt is connected with the sample adding device, and the transverse synchronous belt moves to drive the sample adding device to horizontally move along the transverse guide rail; the sample adding device comprises a sample adding mounting plate, a plurality of sample adding driving mechanisms, a liquid level detection plate and a plurality of gun heads, wherein the sample adding mounting plate is connected with the transverse synchronous belt and the transverse guide rail and can horizontally move along the transverse guide rail; the device comprises a sample feeding driving mechanism, a liquid level detection plate, a data processing and control module, a horizontal driving mechanism, a sample feeding driving mechanism and a liquid level detection plate, wherein the sample feeding driving mechanism and the liquid level detection plate are all installed on a sample feeding installation plate, each gun head is correspondingly connected with one sample feeding driving mechanism and vertically moves under the action of the sample feeding driving mechanism, and the horizontal driving mechanism, the sample feeding driving mechanism and the liquid level detection plate are all connected with the data processing and control module. The plunger pump is arranged in the sample adding and transporting module and connected with the gun head, and the plunger pump is mainly used for connecting the plunger pump with the suction head, so that the functions of sucking, transferring and injecting a sample by the suction head are realized. Simultaneously, the functions of gun head detection, liquid level detection and bar code scanning are also accompanied.
Further, reagent reaction module includes reagent reaction support and reagent reaction actuating mechanism, reagent reaction support and reagent reaction actuating mechanism all install in the frame, be provided with optical axis two on the reagent reaction support, the cover has slider two on the optical axis two, and slider two can move along the axis of optical axis two, it places the board to be provided with the reagent strip on slider two, the reagent strip is placed and is provided with the hot plate between board and the slider two, reagent reaction actuating mechanism and hot plate all are connected with data processing and control module, reagent reaction actuating mechanism is connected with conveyer belt three, conveyer belt three is connected with slider two, reagent reaction actuating mechanism can drive conveyer belt three and remove and make slider two carry out the round trip movement. The reagent reaction module is mainly used for heating the reagent strip placing plate, providing a stable environment for reagent and sample reaction, and the driving mechanism pulls the reagent card placing plate through the conveying belt III and the reagent card moves along the optical axis II to achieve the positions of sample adding, puncture, reaction, lighting and the like.
Further, reagent washs separation module and includes reagent washing separation support frame, and reagent washing separation support frame installs in the frame, installs a plurality of groups bar magnet stirring mixing device on the reagent washing separation support frame and punctures the device, and every group bar magnet stirring mixing device corresponds and punctures the device position with a set of and is close to, and bar magnet stirring mixing device and puncture the device homoenergetic and wash the vertical removal on the separation support frame relatively. The magnetic rod stirring and mixing device comprises a magnetic rod stirring and mixing driving mechanism I and a magnetic rod stirring and mixing driving mechanism II, wherein the magnetic rod stirring and mixing driving mechanism I is installed on the reagent cleaning and separating support frame, the magnetic rod stirring and mixing driving mechanism I is connected with a magnetic rod sleeve installation plate, the magnetic rod sleeve installation plate can vertically move under the action of the magnetic rod stirring and mixing driving mechanism I, and a plurality of magnetic rod sleeves are installed on the magnetic rod sleeve installation plate; the second magnetic rod stirring and mixing driving mechanism is located above the magnetic rod sleeve mounting plate and can move synchronously along with the magnetic rod sleeve mounting plate, the second magnetic rod stirring and mixing driving mechanism is connected with a magnetic rod support, the magnetic rod support can vertically move under the action of the second magnetic rod stirring and mixing driving mechanism, a plurality of magnetic rods are installed on the magnetic rod support, and each magnetic rod penetrates through the magnetic rod sleeve mounting plate and can be inserted into the corresponding magnetic rod sleeve. The device of stabbing is including stabbing actuating mechanism, stabs actuating mechanism and installs on reagent washs separation support frame, stabs actuating mechanism and all is connected with and stabs a fixed plate, and stabs a fixed plate and can carry out vertical movement under the effect of stabbing actuating mechanism, stabs and is connected with a plurality of on the piece fixed plate and stabs a piece. The reagent cleaning and separating module is mainly used for completing reagent strip puncture, extracting paramagnetic beads in reaction and completing cleaning and transferring.
The lighting module comprises a lighting driving mechanism and a PMT support, the lighting driving mechanism is installed on the PMT support, a reagent cleaning and separating support frame is provided with a lighting horizontal guide rail and a lighting synchronous belt which are parallel to each other, the PMT support is connected with the lighting horizontal guide rail and the lighting synchronous belt at the same time and can horizontally move along the lighting horizontal guide rail, the lighting driving mechanism is connected with a PMT mounting plate, the PMT mounting plate can vertically move under the action of the lighting driving mechanism, and the PMT mounting plate is provided with a PMT; be provided with daylighting longitudinal rail two on the PMT support, the PMT mounting panel is connected and can carry out vertical migration along daylighting longitudinal rail two with daylighting longitudinal rail two, is connected with the magazine mount pad on the reagent washs the separation support frame, is provided with the PMT magazine on the magazine mount pad. The lighting module is mainly used for collecting and amplifying the luminous reactant, converting the signal into a motor signal and transmitting the motor signal to the data processing and control module.
The invention has the beneficial effects that:
1. the equipment has small volume, is not limited to be used in a hospital center laboratory, can be used in departments such as clinical laboratory, outpatient and emergency treatment, ICU, intracardiac department, urinary system, clinic and the like, and has wide application objects;
2. the scheme is simple to operate, and after the reagent, the sample and the consumable are put in, the instrument performs full-automatic test and outputs a result, so that the full-automatic and intelligent operation can be realized;
3. the scheme automatically records and searches the unused disposable suction head, accurately controls the sample capacity, and can avoid sample pollution caused by repeated use of the suction head and improper cleaning of the suction head, and influence on detection precision;
4. according to the scheme, the magnetic rod protective sleeve on the reagent strip is automatically searched, the magnetic rod is protected from being polluted, the reacted substances are accurately transferred, and the influence on detection precision due to cross pollution caused by extraction of reactants by equipment is avoided;
5. the reagent card adopts a sealing and packaging mode, the corresponding area is punctured when the reagent card is used, the quality guarantee period of the reagent is long, the reagent is convenient to transport, the formula reagent is quantitatively packaged in the reagent card, the large-dose perfusion is avoided like the traditional equipment, the reagent can be accurately controlled, and the problem that the reagent is exposed for a long time, so that the failure is caused, and the detection result is influenced is avoided;
6. the reagent card is used by a single reagent amount and needs to be punctured for use, so that waste is avoided;
7. the scheme can automatically remove the waste suction heads and the waste magnetic rod sleeve protective sleeves and can temporarily store a large amount of waste consumables; the cleaning of the consumables can be carried out once in a long time, so that the time is saved;
8. the scheme adopts a chemiluminescence technology, PMT acquires information, and the precision of a test result is very high.
Drawings
FIG. 1 is a schematic flow chart of the present invention;
FIG. 2 is a schematic structural view of the main body frame;
FIG. 3 is a schematic view of a sample storage module;
FIG. 4 is a schematic diagram of the tip storage module;
FIG. 5 is a schematic structural view of a sample application transport module;
FIG. 6 is a schematic structural view of a reagent reaction module;
FIG. 7 is a schematic diagram of the structure of a reagent washing and separating module;
FIG. 8 is a schematic structural view of a lighting module;
FIG. 9 is a schematic diagram of a data processing and control module;
fig. 10 is a schematic structural view of the rack.
In the figure: 1-a sample storage module; 1-1-a first driving mechanism; 1-2-code scanning device; 1-3-bearing mounting seats; 1-4-test tube rack; 1-5-test tube; 1-6-test tube induction piece; 1-7-slot type photoelectricity; 1-8-a guide seat; 1-9-a guide bar; 1-10-sample driving wheel; 1-11-rotation axis; 1-12-sample sync wheel; 1-13-synchronous belt b; 3-a suction head storage module; 3-1-a second driving mechanism; 3-2-a second driving mechanism base; 3-3-a suction head storage support frame; 3-4-conveyor belt II; 3-5-a suction head support plate; 3-6-sliding block I; 3-7-a suction head box placing plate; 3-9-a suction head placing rack; 3-10-a suction head placing plate; 3-11-optical axis one; 4-sample loading and transporting module; 4-1-a sample-adding support column; 4-2-sample adding beam; 4-3-transverse guide rails; 4-4-sample adding reset optical coupler; 4-5-sample loading belt wheel; 4-6-transverse synchronous belt; 4-7-sample adding driving wheel; 4-8-a transverse drive mechanism; 4-9-plunger pump support; 4-10-transverse drag chain; 4-11-plunger pump; 4-12-sample loading mounting plate; 4-13-sample application driving mechanism; 4-14-longitudinal guide rail for sample loading; 4-15-sample adding detection optocoupler; 4-16-bar code scanner; 4-17-sample loading slide block; 4-18-liquid level detection plate; 4-19-a suction head grabbing detection optocoupler; 4-20-sample adding driving mechanism supporting seat; 4-21-sample loading drag chain; 4-22-gun head; 4-23-a suction head; 5-a reagent reaction module; 5-1-reagent reaction drive mechanism mounting plate; 5-2-reagent reaction drive mechanism; 5-3-reagent reaction support column; 5-4-reagent reaction holder; 5-5-optical axis two; 5-6-waste baffles; 5-7-a tow chain mounting plate; 5-8-a second sliding block; 5-9-temperature sensor; 5-10-temperature insurance; 5-11-detecting the reagent strip plate; 5-12-heating plate; 5-13-reagent strip placing plate; 5-14-reagent strip; 5-15-reagent reaction detection optical coupler; 5-16-detecting the optical coupling baffle plate by reagent reaction; 5-17-synchronous belt connecting plate; 5-18-reagent reaction driving wheel; 5-19-conveyor belt III; 5-20-reagent reaction driven wheel; 6-reagent cleaning and separating module; 6-1-support section bar; 6-2-Z direction optical coupling induction sheet; 6-3-guide rail connection plate; 6-4-Z directional optical couplers; 6-5-cleaning a separating beam by a reagent; 6-6-a first magnetic rod stirring and uniformly mixing driving mechanism; 6-7-a lancing drive mechanism; 6-8-magnetic rod sleeve; 6-9-magnetic bar; 6-10-magnetic bar sleeve mounting plate; 6-11-bar magnet holder; 6-12-a magnetic rod stirring and uniformly mixing driving mechanism II; 6-13-first magnetic bar Z-direction optical coupling induction sheet; 6-14-first Z-directional optical coupler of the magnetic rod; 6-15-magnetic bar Z-direction optical coupling induction sheet II; 6-16-a second Z-directional optical coupler of the magnetic rod; 6-17-a piercing member; 6-18-a piercing member securing plate; 6-19-puncturing the optical coupling induction sheet; 6-20-puncturing the optocoupler; 6-21-longitudinal guide rail; 7-a lighting module; 7-2-a lighting transverse driving mechanism; 7-3-a lighting driving wheel; 7-4-lighting synchronous belt; 7-5-a lighting horizontal guide rail; 7-6-PMT support; 7-7-Z direction optical coupling baffle sheets; 7-8-Z directional optical couplers; 7-9-a daylighting drive mechanism; 7-10-PMT mounting plate; 7-11-PMT body; 7-12-a lighting driven wheel; 7-13-transverse optical coupling baffle sheet; 7-14-cassette mount; 7-15-transverse optical coupler; 8-a data processing and control module; 8-1-electrical mounting board; 8-2-main control board; 8-3-expansion board; 8-4-fan; 8-5-a switching power supply; 8-6-USB interface; 8-7-network cable interface; 8-8-power line; 8-9-temperature control plate; 9-a frame; 9-1-a rack bottom plate; 9-2-section bar support; 9-3-removing the bracket by the waste suction head.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the description of the present application, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a particular orientation, be constructed in a particular orientation, and be operated, and thus should not be construed as limiting the present application.
The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless otherwise specified.
In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
Example (b):
as shown in figures 1 and 10, the full-automatic chemiluminescence immunoassay analyzer comprises a frame 9, wherein the frame 9 mainly comprises a frame bottom plate 9-1, a section bar bracket 9-2 and a waste suction head removing bracket 9-3, the section bar bracket 9-2 and the waste suction head removing bracket 9-3 are both fixed on the frame bottom plate 9-1, the frame 9 is used as a frame structure of the whole analyzer and mainly used for providing support for the whole analyzer, removing and temporarily storing waste suction heads and waste magnetic rod sleeves, meanwhile, the waste suction head removing bracket 9-3 is matched with the gun head of the sample adding and transporting module to complete the removing function of the waste suction head, and the waste suction head removing support 9-3 is matched with a quadrangular knife of the reagent cleaning and separating module to complete the removing function of the waste magnetic rod sleeve, and a drawer arranged below the waste suction head removing support 9-3 has the function of temporarily storing the waste suction head and the waste magnetic rod sleeve.
As shown in fig. 2, a sample storage module 1, a reagent cleaning and separating module 6, a reagent reaction module 5, a sample adding and transporting module 4, a lighting module 7, a suction head storage module 3 and a data processing and controlling module 8 are arranged on a frame 9, wherein the sample storage module 1, the reagent cleaning and separating module 6, the reagent reaction module 5, the sample adding and transporting module 4, the lighting module 7 and the suction head storage module 3 are all connected with the data processing and controlling module 8 at the same time, the suction head storage module 3 is arranged between the sample storage module 1 and the reagent reaction module 5, the reagent cleaning and separating module 6 is arranged between the sample adding and transporting module 4 and the lighting module 7, and the sample adding and transporting module 4 is arranged between the reagent reaction module 5 and the reagent cleaning and separating module 6.
As shown in fig. 3, the sample storage module 1 comprises a first driving mechanism 1-1, the first driving mechanism 1-1 is installed on a rack 9, the first driving mechanism 1-1 is connected with a circulating device, the circulating device can realize circulating motion under the action of the first driving mechanism 1-1, the outer wall of the circulating device is provided with a plurality of test tube racks 1-4, and the test tube racks 1-4 can move along with the circulating device; a code scanning device 1-2 is arranged outside the circulating device, the code scanning device 1-2 can scan the codes of the test tubes 1-5 in the test tube racks 1-4, and the driving mechanism I1-1 and the code scanning device 1-2 are connected with a data processing and control module 8. The code scanning device 1-2 is a bar code scanner, and can scan the bar code on the sample rack and identify and record the bar code on the test tube.
The first driving mechanism 1-1 is connected with the sample synchronizing wheel 1-12 of the sample driving wheel 1-10 through a synchronous belt b1-13, and the sample driving wheel 1-10 is connected with the sample synchronizing wheel 1-12 through a rotating shaft 1-11. The top of the sample driving wheel 1-10 is provided with a bearing mounting seat 1-3. The sample driving wheel 1-10 is sleeved with a synchronous belt a, the synchronous belt a is sleeved with an idler wheel, the sample driving wheel 1-10 and the idler wheel are used for tensioning the synchronous belt a, the idler wheel is mounted on an idler wheel support, the idler wheel support is connected with a tensioning seat, and the tensioning seat is used for adjusting so that the synchronous belt a can achieve proper tensioning force. The sample driving wheels 1-10, the idle wheel and the synchronous belt a form a circulating device, and the test tube racks 1-4 are uniformly arranged on the outer wall of the synchronous belt a according to a certain sequence and can circularly move along with the synchronous belt a.
In order to ensure the smooth running of the device, a guide device is arranged outside the circulating device. The guide device comprises guide seats 1-8, guide rods 1-9 are arranged in the guide seats 1-8, and the guide rods 1-9 penetrate through the guide seats 1-8 and then are attached to the outer wall, facing the outside of the circulating device, of the test tube rack 1-4. Each test tube rack 1-4 passing by is guided by a guide bar 1-9. The test tube racks 1-4 are ensured to be in tight and smooth circular motion along with the synchronous belt a. Test tube response piece 1-6 and cell type photoelectricity 1-7 are all installed to 3 bottoms of test-tube rack for detect the test tube position in every test-tube rack 3, guarantee that test tube 1-5 inserts and targets in place, follow-up work can continuously go on.
As shown in figure 4, the suction head storage module 3 comprises a driving mechanism II 3-1 and a suction head storage support frame 3-3, the driving mechanism II 3-1 and the suction head storage support frame 3-3 are both arranged on a frame 9, an optical axis I3-11 is arranged on the suction head storage support frame 3-3, a slide block I3-6 is sleeved on the optical axis I3-11, the first sliding block 3-6 can move along the axis of the first optical axis 3-11, a suction head storage device is arranged on the first sliding block 3-6, the second driving mechanism 3-1 is connected with the second conveying belt 3-4, the second conveying belt 3-4 is connected with the first sliding block 3-6, the second driving mechanism 3-1 can drive the second conveying belt 3-4 to move so that the first sliding block 3-6 moves back and forth, and the second driving mechanism 3-1 is connected with the data processing and control module 8. The bottom of the driving mechanism II 3-1 is provided with a driving mechanism II base 3-2 to increase the installation stability, a suction head box placing plate 3-7 is arranged on the sliding block I3-6, and a suction head storage device is arranged on the suction head box placing plate 3-7. The suction head storage device is composed of suction head support plates 3-5, suction head placing frames 3-9 and suction head placing plates 3-10, the suction head support plates 3-5 are installed on first sliding blocks 3-6, the suction head placing frames 3-9 are fixed on the suction head support plates 3-5, the suction head placing plates 3-10 are located inside an area formed by the suction head placing frames 3-9, suction heads can be arranged in the suction head placing plates 3-10 in a matrix mode and move along the axial direction of a first optical axis 3-11 to provide disposable suction heads, and the efficiency of the sample adding transportation module is improved.
As shown in fig. 5, the sample-adding transportation module 4 includes a sample-adding transportation supporting mechanism, the sample-adding transportation supporting mechanism is installed on the frame 9, a sample-adding device is arranged on the sample-adding transportation supporting mechanism, the sample-adding device can horizontally move along the sample-adding transportation supporting mechanism, the sample-adding device can vertically move along the axis of the sample-adding device, and the sample-adding device is connected with the data processing and control module 8. The sample adding and transporting support mechanism comprises a transverse guide rail 4-3 and a transverse driving mechanism 4-8, the transverse guide rail 4-3 is fixed with the sample adding and transporting support mechanism, the sample adding and transporting support mechanism is provided with a plurality of sample adding belt wheels 4-5, the transverse driving mechanism 4-8 is connected with a transverse synchronous belt 4-6, the transverse synchronous belt 4-6 is simultaneously connected with the sample adding belt wheels 4-5, the transverse synchronous belt 4-6 is connected with a sample adding device, and the transverse synchronous belt 4-6 moves to drive the sample adding device to horizontally move along the transverse guide rail 4-3; the sample adding device comprises a sample adding mounting plate 4-12, a plurality of sample adding driving mechanisms 4-13, a liquid level detection plate 4-18 and a plurality of gun heads 4-22, wherein the sample adding mounting plate 4-12 is connected with a transverse synchronous belt 4-6 and a transverse guide rail 4-3 and can horizontally move along the transverse guide rail 4-3; the sample adding driving mechanism 4-13 and the liquid level detection plate 4-18 are both arranged on the sample adding mounting plate 4-12, and a sample adding driving mechanism supporting seat 4-20 is arranged below the sample adding driving mechanism 4-13 and used for supporting a screw rod of the sample adding driving mechanism 4-13. Each gun head 4-22 is correspondingly connected with one sample adding driving mechanism 4-13 and vertically moves under the action of the sample adding driving mechanism 4-13, and the transverse driving mechanism 4-8, the sample adding driving mechanism 4-13 and the liquid level detection plate 4-18 are all connected with the data processing and control module 8. The longitudinal movement of the sample adding driving mechanism 4-13 is realized and limited by a sample adding longitudinal guide rail 4-14 and a sample adding slide block 4-17, and a bar code scanner 4-16 is arranged on the sample adding mounting plate 4-12.
The sample adding transportation supporting mechanism is composed of two sample adding supporting columns 4-1 and a sample adding beam 4-2, the sample adding beam 4-2 is horizontally arranged, the top ends of the sample adding supporting columns 4-1 are vertically fixed with the sample adding beam 4-2, and a sample adding reset optical coupler 4-4 is arranged on the side wall of the sample adding beam 4-2 and used for limiting the horizontal movement of a sample adding device. An output shaft of the transverse driving mechanism 4-8 is provided with a sample adding driving wheel 4-7, a transverse synchronous belt 4-6 is sleeved on the sample adding driving wheel 4-7, and the transverse driving mechanism 4-8 rotates to drive the sample adding driving wheel 4-7 to rotate, so that the transverse synchronous belt 4-6 rotates. Adding or removing suction heads 4-23 on the gun heads 4-22 according to working requirements, arranging plunger pump supports 4-9 above the sample adding beam 4-2, fixing the plunger pump supports 4-9 and the sample adding beam 4-2, installing plunger pumps 4-11 on the plunger pump supports 4-9, connecting the plunger pumps 4-11 and the gun heads 4-22, fixing the plunger pumps 4-11 on the plunger pump supports 4-9, connecting the plunger pumps with the gun heads 4-22 through guide pipes, and completely discharging samples of each gun head 4-22 onto a reagent clamping strip through the work of the plunger pumps 4-11, thereby ensuring the accuracy of subsequent tests and avoiding sample residues.
Each group of gun heads 4-22 is provided with a liquid level detection plate 4-18 which can realize accurate sample suction of different sample volumes when used together with a conductive suction head so as to improve the sample suction precision of the whole device and further improve the overall performance of a matched instrument. And the state of the suction head is monitored in real time, so that the matched instrument is more intelligent. Because of the requirements of moving distance and quantity, a plurality of groups of cables need to be designed, and the cables have corresponding lengths, in order to prevent the cables from interfering in the working process of the device, the sample-adding mounting plate 4-12 is provided with sample-adding drag chains 4-21, 4-21 connected with the gun heads 4-22; a transverse drag chain 4-10 is arranged on the sample adding beam 4-2, and the transverse drag chain 4-10 is connected with a transverse driving mechanism 4-8. In order to control the moving precision, a sample adding detection optocoupler 4-15 and a suction head grabbing detection optocoupler 4-19 are also arranged.
The sample adding main body is dragged to run on the guide rail through the transverse driving mechanism 4-8 and the transverse synchronous belt 4-6, the plunger pump 4-11 is sucked and sample adding position is controlled through the control system, sample dosage is controlled at high precision, samples are processed, after the gun head grabs the suction head, the suction head grabs and detects whether the detection optical coupling is in place of the gun head, the gun head is 4 in one group and can run in parallel.
As shown in FIG. 6, the reagent reaction module 5 comprises a reagent reaction support 5-4 and a reagent reaction driving mechanism 5-2, the reagent reaction support 5-4 and the reagent reaction driving mechanism 5-2 are both mounted on a frame 9, the reagent reaction support 5-4 is provided with an optical axis II 5-5, the optical axis II 5-5 is sleeved with a sliding block II 5-8, the sliding block II 5-8 can move along the axis of the optical axis II 5-5, the sliding block II 5-8 is provided with a reagent strip placing plate 5-13, a heating plate 5-12 is arranged between the reagent strip placing plate 5-13 and the sliding block II 5-8, the reagent reaction driving mechanism 5-2 and the heating plate 5-12 are both connected with a data processing and control module 8, the reagent reaction driving mechanism 5-2 is connected with a conveyor belt III 5-19, the third conveyor belt 5-19 is connected with the second sliding block 5-8, and the reagent reaction driving mechanism 5-2 can drive the third conveyor belt 5-19 to move so that the second sliding block 5-8 moves back and forth. The reagent reaction driving mechanism 5-2 is arranged on the frame 9 through a reagent reaction driving mechanism mounting plate 5-1, and the reagent reaction bracket 5-4 is fixed with the frame through a reagent reaction bracket supporting column 5-3. The reagent strip placing plate 5-13 is provided with reagent strips 5-14, the reagent reaction support 5-4 is provided with a reagent reaction driving wheel 5-18 and a reagent reaction driven wheel 5-20, a conveying belt III 5-19 is arranged between the reagent reaction driving wheel 5-18 and the reagent reaction driven wheel 5-20, the conveying belt III 5-19 is simultaneously sleeved on the reagent reaction driving wheel 5-18 and the reagent reaction driven wheel 5-20 and tightened, the conveying belt III 5-19 is connected with a synchronous belt connecting plate 5-17, and the synchronous belt connecting plate 5-17 is connected with a sliding block II 5-8. And reagent reaction detection optocouplers 5-15 and reagent reaction detection optocoupler baffles 5-16 are arranged on the synchronous belt connecting plates 5-17 and used for realizing the control of movement. The heating plate 5-12 is connected with a temperature sensor 5-9 and a temperature safety 5-10 for monitoring the temperature and preventing damage caused by high temperature. Reagent strip placing plates 5-13 are provided with reagent strip detecting plates 5-11, end parts of the second sliding blocks 5-8 are provided with drag chain mounting plates 5-7 and waste baffle plates 5-6, and the drag chain mounting plates 5-7 and the waste baffle plates 5-6 are both positioned between the second sliding blocks 5-8 and the reagent reaction driving mechanisms 5-2. The reagent strip placing plates 5-13 are heated by heating plates 5-12, temperature sensors 5-9 collect temperature information, a control system effectively controls the temperature of the reagent strips, the placed reagent strips 5-14 are subjected to temperature processing, and a subsequent lighting module is guaranteed to smoothly read optical information.
As shown in fig. 7, the reagent cleaning and separating module 6 includes a reagent cleaning and separating support frame, the reagent cleaning and separating support frame is installed on the rack 9, a plurality of groups of magnetic rod stirring and mixing devices and a puncturing device are installed on the reagent cleaning and separating support frame, each group of magnetic rod stirring and mixing devices corresponds to one group of puncturing device and is close to the position of the puncturing device, and the magnetic rod stirring and mixing devices and the puncturing device can vertically move relative to the reagent cleaning and separating support frame. The magnetic rod stirring and mixing device comprises a first magnetic rod stirring and mixing driving mechanism 6-6 and a second magnetic rod stirring and mixing driving mechanism 6-12, the first magnetic rod stirring and mixing driving mechanism 6-6 is a driving mechanism for controlling the action of a magnetic rod sleeve 6-8, the second magnetic rod stirring and mixing driving mechanism 6-12 is a driving mechanism for controlling the action of a magnetic rod 6-9, the first magnetic rod stirring and mixing driving mechanism 6-6 is installed on the reagent cleaning and separating support frame, the first magnetic rod stirring and mixing driving mechanism 6-6 is connected with a magnetic rod sleeve installation plate 6-10, the magnetic rod sleeve installation plate 6-10 can vertically move under the action of the first magnetic rod stirring and mixing driving mechanism 6-6, and a plurality of magnetic rod sleeves 6-8 are installed on the magnetic rod sleeve installation plate 6-10; the second magnetic rod stirring and mixing driving mechanism 6-12 is located above the second magnetic rod sleeve mounting plate 6-10 and can synchronously move along with the second magnetic rod sleeve mounting plate 6-10, the second magnetic rod stirring and mixing driving mechanism 6-12 is connected with a second magnetic rod support 6-11, the second magnetic rod support 6-11 can vertically move under the action of the second magnetic rod stirring and mixing driving mechanism 6-12, a plurality of magnetic rods 6-9 are mounted on the second magnetic rod support 6-11, and each magnetic rod 6-9 penetrates through the second magnetic rod sleeve mounting plate 6-10 and can be inserted into the corresponding magnetic rod sleeve 6-8. The puncturing device comprises puncturing driving mechanisms 6-7, the puncturing driving mechanisms 6-7 are installed on the reagent cleaning and separating support frame, the puncturing driving mechanisms 6-7 are connected with puncturing piece fixing plates 6-18, the puncturing piece fixing plates 6-18 can vertically move under the action of the puncturing driving mechanisms 6-7, and a plurality of puncturing pieces 6-17 are connected to the puncturing piece fixing plates 6-18. The reagent cleaning and separating support frame is composed of two support sectional materials 6-1 and a reagent cleaning and separating beam 6-5, the reagent cleaning and separating beam 6-5 is arranged between the support sectional materials 6-1, and the top of the support sectional material 6-1 is vertically fixed with the reagent cleaning and separating beam 6-5. The longitudinal guide rails 6-21 are used for guiding and limiting the longitudinal movement of the magnetic rod stirring and blending device. The guide rail connecting plate 6-3 is arranged on the side wall of the reagent cleaning and separating beam 6-5 and used for connecting the longitudinal guide rail 6-21, and the Z-direction optical coupler induction sheet 6-2 and the Z-direction optical coupler 6-4 are used for controlling the longitudinal movement of the magnetic rod sleeve 6-8. The first magnetic rod Z-direction optical coupling induction sheet 6-13, the first magnetic rod Z-direction optical coupling 6-14, the second magnetic rod Z-direction optical coupling induction sheet 6-15 and the second magnetic rod Z-direction optical coupling 6-16 are used for controlling the longitudinal movement of the magnetic rods 6-9. The poking optical coupling induction sheets 6-19 and the poking optical couplings 6-20 control the movement of the poking pieces 6-17, and the poking pieces 6-17 are preferably quadrangular knives.
The reagent strip of the reagent reaction module reaches a puncture position under the traction of the motor drive. The puncture driving mechanism 6-7 acts, and the puncture piece 6-17 punctures the sealing film on the reagent strip downwards; after the reagent strip of the reagent reaction module reacts, the reagent strip reaches a magnetic bar adsorption position under the driving and traction of a motor, a magnetic bar motor and a magnetic bar sleeve motor act together to complete the stirring of the reagent, the adsorption of paramagnetic magnetic beads, the cleaning and the placement of the magnetic beads in a lighting area for a detection module to acquire optical information; the magnetic rod sleeve is a disposable consumable material, so that cross contamination can be avoided; the embodiment consists of four groups of puncture separation, and can be operated independently in parallel.
As shown in fig. 8, the lighting module 7 comprises a lighting driving mechanism 7-9 and a PMT bracket 7-6, the lighting driving mechanism 7-9 is installed on the PMT bracket 7-6, a lighting horizontal guide rail 7-5 and a lighting synchronous belt 7-4 which are parallel to each other are arranged on the reagent cleaning separation support frame, the PMT bracket 7-6 is simultaneously connected with the lighting horizontal guide rail 7-5 and the lighting synchronous belt 7-4 and can horizontally move along the lighting horizontal guide rail 7-5, the lighting driving mechanism 7-9 is connected with a PMT mounting plate 7-10, the PMT mounting plate 7-10 can vertically move under the action of the lighting driving mechanism, and a PMT main body 7-11 is arranged on the PMT mounting plate 7-10; the PMT support 7-6 is provided with a lighting longitudinal guide rail, the PMT mounting plate 7-10 is connected with the lighting longitudinal guide rail and can vertically move along the lighting longitudinal guide rail, the reagent cleaning separation support frame is connected with the cassette mounting seats 7-14, and the cassette mounting seats 7-14 are provided with PMT cassettes. The lighting module is arranged on the back of the reagent cleaning and separating module and shares a reagent cleaning and separating beam 6-5 with the reagent cleaning and separating beam 6-5, a lighting driving wheel 7-3 and a lighting driven wheel 7-12 are arranged on the reagent cleaning and separating beam 6-5, a lighting synchronous belt 7-4 is simultaneously connected and tightened with the lighting driving wheel 7-3 and the lighting driven wheel 7-12, the lighting driving wheel 7-3 is connected with a lighting transverse driving mechanism 7-2, and a transverse optical coupling baffle 7-13 and a transverse optical coupling 7-15 are used for controlling the transverse movement of the PMT main body 7-11. The Z-direction optical coupler baffle 7-7 and the Z-direction optical coupler 7-8 limit vertical movement of the PMT mounting plate 7-10, the PMT main body 7-11 moves to a reagent card lighting position along a lighting horizontal guide rail 7-5 under the driving of a lighting transverse driving mechanism 7-2 and a lighting synchronous belt 7-4, the lighting driving mechanism 7-9 drives the PMT main body 7-11 to move along the longitudinal direction, and the PMT main body 7-11 reaches a proper lighting height, reads optical information, amplifies and converts the optical information into an electric signal.
As shown in fig. 9, the hardware structure of the data processing and control module 8 is composed of an electrical installation board 8-1, a main control board 8-2, an expansion board 8-3, a fan 8-4, a switching power supply 8-5, a USB interface 8-6, a network cable interface 8-7, a power line 8-8 and a temperature control board 8-9, the data processing and control module is composed of various control elements, the main control board 8-2 and the expansion board 8-3 can complete various controls and data analyses of the scheme, the result is measured and calculated, the temperature control board 8-9 can control the temperature of the reagent card placing board machine, and the data processing and control module comprises various interfaces.
After the analyzer is started, the reagent reaction module can be preheated. After the samples and corresponding single-person reagent strips, consumables and the like are manually put in, the sample storage module automatically sorts and positions the samples, and the bar codes scan, identify and record sample information. The reagent reaction module heats the reagent clamping strip and carries out real-time feedback regulation and control. The reagent cleaning and separating module automatically punctures the sealing film of the corresponding test item of the reagent card strip. The sample adding transportation module automatically grabs the disposable suction head and absorbs a sample, after the sample solution is transported to the upper part of the corresponding reagent card strip, the code is scanned to identify the information of the reagent card, the unique corresponding relation between the sample and the reagent card is completed, the sample adding assembly drops the sample solution to the corresponding reaction area of the reagent card, and the disposable suction head is automatically removed. The sample and the reagent are sufficiently reacted in the reagent reaction module. The reagent cleaning and separating module grabs a magnetic rod protective sleeve at the front end of the reagent card, after reaction is completed, the reagent card is moved to a reagent strip sample reaction area, paramagnetic particles combined with the sample are adsorbed onto the magnetic rod protective sleeve through the magnetic rod, cleaning is carried out for different times according to different projects, and impurities are removed. And after the reagent cleaning and separating module runs to a lighting area, the magnetic rod is extracted, and the paramagnetic particles fall off. The detection module is used for lighting in a lighting area, converting an optical signal into an electric signal, and performing data analysis and outputting a result through the control system. The reagent cleaning and separating module automatically removes the magnetic bar protective sleeve. The scheme can automatically grab and install the disposable sucker and the magnetic bar protective sleeve; the single test has large sample amount, and can automatically sort and identify samples and record information into the system; automatic sample adding, parallel detection of a plurality of channels and automatic reagent puncturing; providing a constant-temperature immune reaction environment, and carrying out real-time monitoring, feedback and adjustment; automatically identifying related information such as reagent card detection items; the immune reaction calibration object is automatically cleaned for different times according to different requirements and transferred, PMT automatic lighting and signal transmission and analysis can be carried out, multiple detection and comparison can be carried out, and after detection is finished, the suction head and the magnetic rod protective sleeve are automatically removed. The equipment is small in size, is not limited to be used in a hospital center laboratory, can be used in departments such as clinical laboratory, outpatient and emergency treatment, ICU, intracardiac department, urinary department, clinic and the like, and is widely applicable to objects. This scheme easy operation puts into reagent, sample and consumptive material back, and the full automatic test of instrument and output result can full-automatic and intelligent operation, and its automatic recording seeks the disposable suction head that does not use, and accurate control sample capacity avoids the used repeatedly of suction head and suction head washing not in place, and the sample pollution that causes influences the detection precision. The magnetic rod protective sleeve on the reagent strip can be automatically searched, the magnetic rod is protected from being polluted, the reacted substances are accurately transferred, and the cross contamination caused by the extraction of the reactants by equipment is avoided, so that the detection precision is not influenced; the reagent card adopts a sealing and packaging mode, the corresponding area is punctured when the reagent card is used, the quality guarantee period of the reagent is long, the reagent is convenient to transport, the formula reagent is quantitatively packaged in the reagent card, the large-dose perfusion is avoided unlike the traditional equipment, the reagent can be accurately controlled, and the failure caused by long-term exposure of the reagent and the influence on the detection result are avoided; the reagent card is used by a single person and needs to be punctured before use, and the reagent amount can be confirmed, so that waste is avoided; the equipment can automatically remove the waste suction heads and the waste magnetic rod sleeve protective sleeves, and can temporarily store a large amount of waste consumables, and the consumables can be cleaned once in a long time, so that the time is saved; the equipment adopts a chemiluminescence technology, PMT acquires information, and the precision of a test result is very high.
The above-mentioned embodiments only express the specific embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.

Claims (10)

1. The full-automatic chemiluminescence immunoassay analyzer comprises a rack (9) and is characterized in that a sample storage module (1), a reagent cleaning and separating module (6), a reagent reaction module (5), a sample adding transportation module (4), a lighting module (7), a suction head storage module (3) and a data processing and control module (8) are arranged on the rack (9), wherein the sample storage module (1), the reagent cleaning and separating module (6), the reagent reaction module (5), the sample adding transportation module (4), the lighting module (7) and the suction head storage module (3) are simultaneously connected with the data processing and control module (8), the suction head storage module (3) is arranged between the sample storage module (1) and the reagent reaction module (5), the reagent cleaning and separating module (6) is arranged between the sample adding transportation module (4) and the lighting module (7), the sample feeding and transporting module (4) is arranged between the reagent reaction module (5) and the reagent cleaning and separating module (6).
2. The full-automatic chemiluminescence immunoassay analyzer according to claim 1, wherein the sample storage module (1) comprises a first driving mechanism (1-1), the first driving mechanism (1-1) is mounted on the rack (9), the first driving mechanism (1-1) is connected with a circulating device, the circulating device can realize circulating motion under the action of the first driving mechanism (1-1), a plurality of test tube racks (1-4) are mounted on the outer wall of the circulating device, and the test tube racks (1-4) can move along with the circulating device; a code scanning device (1-2) is arranged outside the circulating device, the code scanning device (1-2) can scan the test tubes (1-5) in the test tube racks (1-4), and a driving mechanism I (1-1) and the code scanning device (1-2) are connected with a data processing and control module (8).
3. The full-automatic chemiluminescence immunoassay analyzer according to claim 1, wherein the suction head storage module (3) comprises a second driving mechanism (3-1) and a suction head storage support frame (3-3), the second driving mechanism (3-1) and the suction head storage support frame (3-3) are both mounted on the rack (9), a first optical axis (3-11) is arranged on the suction head storage support frame (3-3), a first sliding block (3-6) is sleeved on the first optical axis (3-11), the first sliding block (3-6) can move along the axis of the first optical axis (3-11), a suction head storage device is arranged on the first sliding block (3-6), the second driving mechanism (3-1) is connected with a second conveying belt (3-4), and the second conveying belt (3-4) is connected with the first sliding block (3-6), the driving mechanism II (3-1) can drive the conveyor belt II (3-4) to move so that the sliding block I (3-6) moves back and forth, and the driving mechanism II (3-1) is connected with the data processing and control module (8).
4. The full-automatic chemiluminescence immunoassay analyzer of claim 1, wherein the sample feeding transportation module (4) comprises a sample feeding transportation support mechanism, the sample feeding transportation support mechanism is mounted on the frame (9), a sample feeding device is arranged on the sample feeding transportation support mechanism, the sample feeding device can horizontally move along the sample feeding transportation support mechanism, the sample feeding device can vertically move along the axis of the sample feeding device, and the sample feeding device is connected with the data processing and control module (8).
5. The full-automatic chemiluminescence immunoassay analyzer according to claim 4, wherein the sample feeding transportation support mechanism comprises a transverse guide rail (4-3) and a transverse driving mechanism (4-8), the transverse guide rail (4-3) is fixed with the sample feeding transportation support mechanism, a plurality of sample feeding belt wheels (4-5) are arranged on the sample feeding transportation support mechanism, a transverse synchronous belt (4-6) is connected to the transverse driving mechanism (4-8), the transverse synchronous belt (4-6) is simultaneously connected with the sample feeding belt wheels (4-5), the transverse synchronous belt (4-6) is connected with the sample feeding device, and the transverse synchronous belt (4-6) moves to drive the sample feeding device to move horizontally along the transverse guide rail (4-3); the sample adding device comprises a sample adding mounting plate (4-12), a plurality of sample adding driving mechanisms (4-13), a liquid level detection plate (4-18) and a plurality of gun heads (4-22), wherein the sample adding mounting plate (4-12) is connected with a transverse synchronous belt (4-6) and a transverse guide rail (4-3) and can horizontally move along the transverse guide rail (4-3); the sample adding driving mechanism (4-13) and the liquid level detection plate (4-18) are arranged on the sample adding mounting plate (4-12), each gun head (4-22) is correspondingly connected with one sample adding driving mechanism (4-13) and vertically moves under the action of the sample adding driving mechanism (4-13), and the transverse driving mechanism (4-8), the sample adding driving mechanism (4-13) and the liquid level detection plate (4-18) are all connected with the data processing and control module (8).
6. The full-automatic chemiluminescence immunoassay analyzer according to claim 1, wherein the reagent reaction module (5) comprises a reagent reaction support (5-4) and a reagent reaction driving mechanism (5-2), the reagent reaction support (5-4) and the reagent reaction driving mechanism (5-2) are both mounted on a frame (9), a second optical axis (5-5) is arranged on the reagent reaction support (5-4), a second sliding block (5-8) is sleeved on the second optical axis (5-5), the second sliding block (5-8) can move along the axis of the second optical axis (5-5), a reagent strip placing plate (5-13) is arranged on the second sliding block (5-8), and a heating plate (5-12) is arranged between the reagent strip placing plate (5-13) and the second sliding block (5-8), the reagent reaction driving mechanism (5-2) and the heating plate (5-12) are connected with the data processing and control module (8), the reagent reaction driving mechanism (5-2) is connected with a third conveyor belt (5-19), the third conveyor belt (5-19) is connected with a second sliding block (5-8), and the reagent reaction driving mechanism (5-2) can drive the third conveyor belt (5-19) to move so that the second sliding block (5-8) can move back and forth.
7. The full-automatic chemiluminescence immunoassay analyzer according to any one of claims 1 to 6, wherein the reagent cleaning and separating module (6) comprises a reagent cleaning and separating support frame, the reagent cleaning and separating support frame is mounted on the rack (9), a plurality of groups of magnetic rod stirring and mixing devices and puncturing devices are mounted on the reagent cleaning and separating support frame, each group of magnetic rod stirring and mixing devices is correspondingly close to one group of puncturing devices, the magnetic rod stirring and mixing devices and the puncturing devices can vertically move relative to the reagent cleaning and separating support frame, and the magnetic rod stirring and mixing devices and the puncturing devices are connected with the data processing and control module (8).
8. The full-automatic chemiluminescence immunoassay analyzer according to claim 7, wherein the magnetic rod stirring and mixing device comprises a magnetic rod stirring and mixing driving mechanism I (6-6) and a magnetic rod stirring and mixing driving mechanism II (6-12), and the magnetic rod stirring and mixing driving mechanism I (6-6) and the magnetic rod stirring and mixing driving mechanism II (6-12) are connected with the data processing and control module (8); a first magnetic rod stirring and mixing driving mechanism (6-6) is installed on the reagent cleaning and separating support frame, the first magnetic rod stirring and mixing driving mechanism (6-6) is connected with a first magnetic rod sleeve installation plate (6-10), the first magnetic rod sleeve installation plate (6-10) can vertically move under the action of the first magnetic rod stirring and mixing driving mechanism (6-6), and a plurality of magnetic rod sleeves (6-8) are installed on the first magnetic rod sleeve installation plate (6-10); the second magnetic rod stirring and mixing driving mechanism (6-12) is located above the second magnetic rod sleeve mounting plate (6-10) and can move synchronously along with the second magnetic rod sleeve mounting plate (6-10), the second magnetic rod stirring and mixing driving mechanism (6-12) is connected with a magnetic rod support (6-11), the magnetic rod support (6-11) can vertically move under the action of the second magnetic rod stirring and mixing driving mechanism (6-12), a plurality of magnetic rods (6-9) are mounted on the magnetic rod support (6-11), and each magnetic rod (6-9) penetrates through the magnetic rod sleeve mounting plate (6-10) and can be inserted into the corresponding magnetic rod sleeve (6-8).
9. The full-automatic chemiluminescence immunoassay analyzer according to claim 7, wherein the lancing device comprises a lancing drive mechanism (6-7), the lancing drive mechanism (6-7) is installed on the reagent washing separation support frame and connected with the data processing and control module (8), the lancing drive mechanism (6-7) is connected with a lancing member fixing plate (6-18), the lancing member fixing plate (6-18) can vertically move under the action of the lancing drive mechanism (6-7), and a plurality of lancing members (6-17) are connected on the lancing member fixing plate (6-18).
10. The full-automatic chemiluminescence immunoassay analyzer according to claim 7, wherein the lighting module (7) comprises a lighting driving mechanism (7-9) and a PMT bracket (7-6), the lighting driving mechanism (7-9) is installed on the PMT bracket (7-6), a reagent cleaning and separating support frame is provided with a lighting horizontal guide rail (7-5) and a lighting synchronous belt (7-4) which are parallel to each other, the PMT bracket (7-6) is simultaneously connected with the lighting horizontal guide rail (7-5) and the lighting synchronous belt (7-4) and can horizontally move along the lighting horizontal guide rail (7-5), the lighting driving mechanism (7-9) is connected with a PMT mounting plate (7-10), and the PMT mounting plate (7-10) can vertically move under the action of the lighting driving mechanism, PMT main bodies (7-11) are arranged on the PMT mounting plates (7-10), and the PMT main bodies (7-11) and the lighting driving mechanisms (7-9) are connected with a data processing and control module (8); a lighting longitudinal guide rail is arranged on the PMT support (7-6), a PMT mounting plate (7-10) is connected with the lighting longitudinal guide rail and can vertically move along the lighting longitudinal guide rail, a cassette mounting seat (7-14) is connected on the reagent cleaning separation support frame, and a PMT cassette is arranged on the cassette mounting seat (7-14).
CN202110845728.4A 2021-07-26 2021-07-26 Full-automatic chemiluminescence immunoassay analyzer Pending CN113567697A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114441510A (en) * 2022-01-18 2022-05-06 成都宜乐芯生物科技有限公司 POCT full-automatic chemiluminescence device based on multi-channel parallel pretreatment technology
CN114965995A (en) * 2022-05-20 2022-08-30 北京与子成科技有限公司 Immunochromatography analysis detection system and method
CN115825463A (en) * 2023-02-17 2023-03-21 世纪亿康（天津）医疗科技发展有限公司 Blood coagulation detection equipment

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107976553A (en) * 2017-12-18 2018-05-01 置展生物医疗科技（深圳）有限公司 Multi-channel loading mechanical arm
CN109406804A (en) * 2018-10-30 2019-03-01 深圳泰乐德医疗有限公司 Full-automatic chemiluminescence analyzer
CN110133314A (en) * 2018-02-08 2019-08-16 成都深迈瑞医疗电子技术研究院有限公司 Full-automatic chemiluminescence immunoassay analysis meter
CN110749742A (en) * 2019-11-15 2020-02-04 成都宜乐芯生物科技有限公司 POCT full-automatic chemiluminescence device based on active magnetic separation technology
CN111913000A (en) * 2020-06-22 2020-11-10 深圳天深医疗器械有限公司 Full-automatic chemiluminescence immunoassay analyzer
WO2021138963A1 (en) * 2020-01-12 2021-07-15 南京岚煜生物科技有限公司 Fully automatic chemiluminescent immune analyzer
CN113125787A (en) * 2021-04-28 2021-07-16 苏州遵道生物科技有限公司 Full-automatic chemiluminescence immunoassay analyzer

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107976553A (en) * 2017-12-18 2018-05-01 置展生物医疗科技（深圳）有限公司 Multi-channel loading mechanical arm
CN110133314A (en) * 2018-02-08 2019-08-16 成都深迈瑞医疗电子技术研究院有限公司 Full-automatic chemiluminescence immunoassay analysis meter
CN109406804A (en) * 2018-10-30 2019-03-01 深圳泰乐德医疗有限公司 Full-automatic chemiluminescence analyzer
CN110749742A (en) * 2019-11-15 2020-02-04 成都宜乐芯生物科技有限公司 POCT full-automatic chemiluminescence device based on active magnetic separation technology
WO2021138963A1 (en) * 2020-01-12 2021-07-15 南京岚煜生物科技有限公司 Fully automatic chemiluminescent immune analyzer
CN111913000A (en) * 2020-06-22 2020-11-10 深圳天深医疗器械有限公司 Full-automatic chemiluminescence immunoassay analyzer
CN113125787A (en) * 2021-04-28 2021-07-16 苏州遵道生物科技有限公司 Full-automatic chemiluminescence immunoassay analyzer

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114441510A (en) * 2022-01-18 2022-05-06 成都宜乐芯生物科技有限公司 POCT full-automatic chemiluminescence device based on multi-channel parallel pretreatment technology
CN114965995A (en) * 2022-05-20 2022-08-30 北京与子成科技有限公司 Immunochromatography analysis detection system and method
CN114965995B (en) * 2022-05-20 2023-04-11 河南申友医学检验所有限公司 Immunochromatography analysis detection system and method
CN115825463A (en) * 2023-02-17 2023-03-21 世纪亿康（天津）医疗科技发展有限公司 Blood coagulation detection equipment

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