Full-automatic dry-type fluorescence immunoassay appearance
Technical Field
The utility model relates to the technical field of dry fluorescent immunoassay, in particular to a full-automatic dry fluorescent immunoassay instrument.
Background
Early immune products are qualitative products, immune projects are research objects, and a colloidal gold mode is adopted. The colloidal gold product is the star product at this stage. By the antigen-antibody combination principle, almost all protein molecules can be marked theoretically, and the defects are low sensitivity and low accuracy, and only negative and positive can be judged; after the technology is improved, color microsphere marking is adopted, compared with colloidal gold, the sensitivity of the color microsphere marking is improved, the repeatability of a detection result is strong, but the sensitivity is limited; with the development of the technology, the fluorescent microsphere marking technology is applied, the fluorescent microsphere marking has stable luminous intensity, higher biocompatibility and basically no influence of external environment change, and can realize quantitative detection, but has higher requirements on the product technology and higher difficulty.
The fluorescent immunoassay (fluorescenceImmuno technique), also called fluorescent antibody technique, is the earliest technique in the labeled immunoassay, and is a technique based on immunology, biochemistry and microscopy, and a dry immunofluorescence analyzer: the sample solution dropped on one end of the membrane moves to the other end by capillary action (lateral flow (chromatography) based on chromatography), during the movement, the analyte is combined with a receptor (antigen or antibody) fixed on a certain area of the membrane to be immobilized, the irrelevant substances are separated across the area, and then the test result is judged by the detection (qualitative, semi-quantitative and quantitative) of the marker.
The existing fluorescence immunoassay analyzer needs a large number of complicated manual steps to carry out auxiliary detection, and has low detection sensitivity and precision.
Disclosure of Invention
The utility model aims to overcome the problems in the background technology and provide a full-automatic dry-type fluorescence immunoassay analyzer, which ensures that the rapid detection and reaction of products are more controllable, the detection sensitivity and precision are also greatly improved, and the manual steps are also reduced.
The aim of the utility model is mainly realized by the following technical scheme:
the full-automatic dry-type fluorescence immunoassay analyzer comprises a base, wherein a sample module, a reagent card box module, a transportation module, a shaking cap module, an incubation module, a control module, a sample adding module and a suction head box module are arranged on the base, and the sample module, the transportation module, the shaking cap module, the incubation module and the sample adding module are all connected with the control module; the transport module can hook the reagent strips in the reagent cartridge module; the shaking cap pulling module can turn over the test tube in the sample module, and pull out and cover the test tube cap; the incubation module can incubate and collect light for the reagent strips; the sample adding module can absorb samples for sample adding.
In recent years, due to the influence of factors such as environment and the like, the new occurrence of infectious diseases of human bodies is a sudden threat to human health, and the transmission of infectious diseases such as atypical pneumonia, middle east respiratory syndrome, influenza, ebola, zika virus, yellow fever, rift valley fever and the like becomes a global public health problem. With the development of urbanization and the increase of global trade, environmental changes aggravate the transmission risk of natural epidemic infectious diseases, and the prevention and control of infectious diseases are unprecedented. Therefore, the method for detecting infectious diseases quickly and immediately has great significance for the prevention and control of infectious diseases. Meanwhile, the human blood sample is rapidly detected, so that the human disease or potential diseases can be effectively and rapidly detected, and the method has great significance as effective treatment and early-stage prevention of diseases.
POCT products have the advantages of simple operation, portability, intellectualization and the like, and are rapidly developed as in-vitro diagnosis technology in recent years and applied to detection projects of a plurality of subjects. The method can rapidly acquire the detection result, is convenient for patients, and reduces the time for patient diagnosis. Meanwhile, POCT products have the function of continuously using and monitoring the illness state, such as heart disease patients, and the illness state can be quickly known and controlled through detection of interval time. The technical principle applied by POCT is combined with the traditional dry chemical technology, immunochromatography technology, optical information, data analysis technology and the like. The method adopts biomedical technology and chromatographic technology to analyze and process the information of the chromatograph, the spectrum and the biosensor, is applied to immunity and rapid detection, realizes multichannel and automatic detection, improves the field applicability of the device, and presents the advantages of automation and simplicity.
The existing fluorescence immunoassay analyzer needs a large number of complicated manual steps for auxiliary detection, the sensitivity and precision of detection are low due to too much manual participation, the error rate is increased, the judgment of final data is greatly plagued, and meanwhile, the manual workload is large. The reagent strips in the reagent cartridge module are hooked by using the transport module; the shaking cap pulling module is utilized to realize the overturning and shaking of the test tube in the sample module, and the pulling and cap covering of the test tube cap; the incubation module is utilized to realize incubation and lighting of the reagent strips; the sample adding module is used for absorbing the sample to carry out sample adding; the automatic adding, removing and state detecting of the suction head are realized by utilizing a suction head box module; the sample module is utilized to realize automatic sample in-out and sample information reading, so that the product rapid detection and reaction are more controllable, the detection sensitivity and precision are also greatly improved, the manual labor intensity is also reduced, and the lighting modularized design is realized, so that the whole machine integration is more rapid and flexible.
Further, the sample module comprises a sample support, the sample support is arranged on the base, a first driving mechanism, a first transmission assembly, a second driving mechanism, a second transmission assembly, a third driving mechanism and a third transmission assembly are arranged below the sample support, the first transmission assembly is connected with the first driving mechanism and can be driven by the first driving mechanism to transmit, the second transmission assembly is connected with the second driving mechanism and can be driven by the second driving mechanism to transmit, the third transmission assembly is connected with the third driving mechanism and can be driven by the third driving mechanism to transmit, the transmission directions of the first transmission assembly and the third transmission assembly are the same, and the transmission direction of the second transmission assembly is perpendicular to the transmission direction of the third transmission assembly; a sample shifting fork is arranged below the sample support, and is connected with the transmission assembly III and can horizontally move relative to the sample support under the drive of the transmission assembly III.
The first driving mechanism, the second driving mechanism and the third driving mechanism are all preferably motors, the first transmission assembly, the second transmission assembly and the third transmission assembly are respectively combinations of belt wheels and conveyor belts, and the guide assemblies are matched at the positions of each transmission assembly to realize the stability of transmission. According to the scheme, the sample module is used for realizing automatic sample in-out and sample information reading, and the driving mechanism III and the transmission assembly III and the sample rack shifting fork act together to move the sample rack to a preset position; the second driving mechanism and the second transmission assembly act together to move the sample rack to a preset position, and the bar code information is read by a bar code scanning module (including but not limited to a bar code scanner); and after the sample is processed, the first driving mechanism and the transmission assembly act together one by one to move the sample rack to a preset position, and the sensor monitors the position state.
Further, the transportation module comprises a transportation bottom plate, a driving mechanism IV and a transmission assembly IV, wherein the driving mechanism IV and the transmission assembly IV are both fixed on the base, the transmission assembly IV is connected with the driving mechanism IV and can be driven by the driving mechanism IV to transmit, and the transportation bottom plate is connected with the transmission assembly IV and can horizontally move relative to the base under the driving of the transmission assembly IV; the conveying bottom plate is provided with a driving mechanism five, a transmission assembly five, a driving mechanism six, a transmission assembly six, a conveying shifting fork and a conveying bin, wherein the transmission assembly five is connected with the driving mechanism five and can be driven by the driving mechanism five to transmit, and the driving mechanism six is connected with the transmission assembly five and can be driven by the transmission assembly five to horizontally move relative to the conveying bottom plate; the transmission assembly is connected with the driving mechanism in a six-way mode, can be driven by the driving mechanism in a six-way mode, and the conveying shifting fork is connected with the transmission assembly in a six-way mode, can vertically move relative to the conveying bottom plate in a six-way mode, and can be inserted into and pulled out of the conveying bin. The scheme is that the transport module is utilized to realize the transport of reagent strips in the reagent cartridge module, the driving mechanism IV, the driving mechanism V and the driving mechanism VI are all motors, the driving assembly IV and the driving assembly V are respectively combinations of belt wheels and conveying belts, the driving assembly VI adopts a screw nut pair structure, and each set of driving assembly is matched with the guide assembly again to realize the stability of driving. The driving mechanism six and the transmission assembly are driven by the transmission assembly five to horizontally move relative to the transport base plate, the transport shifting fork can move in the vertical direction under the driving of the driving mechanism six, the transport shifting fork can move longitudinally along the guide assembly under the driving of the driving mechanism four, and the transport shifting fork can move transversely along the guide assembly under the driving of the driving mechanism five. Finally, the reagent card can be transported to each preset position and taken out of each preset position of the incubation module and put into a transport bin. The sensor is used for monitoring the state of the reagent card, and the code scale is used for realizing closed-loop control to improve the motion precision of the whole module.
Further, the shaking cap pulling module comprises a shaking base plate fixed with the base, a supporting vertical plate is vertically fixed above the shaking base plate, a driving mechanism seven and a transmission assembly seven are arranged on the supporting vertical plate, the transmission assembly seven is connected with the driving mechanism seven and can be driven by the driving mechanism seven to transmit, the transmission assembly seven is connected with a transfer transverse plate, and the transfer transverse plate can vertically move relative to the supporting vertical plate under the driving of the transmission assembly seven; the transfer transverse plate is provided with a driving mechanism eight and a transmission assembly eight, the transmission assembly eight is connected with the driving mechanism eight and can be driven by the driving mechanism eight to transmit, the transmission assembly eight is connected with clamping jaws, and the clamping jaws can move horizontally relative to the transfer transverse plate under the driving of the transmission assembly eight; be provided with actuating mechanism nine, drive assembly nine, actuating mechanism ten and drive assembly ten on the support riser, drive assembly nine and drive mechanism nine are connected and can drive under the drive of actuating mechanism nine, drive assembly nine is connected with the clamp push pedal, clamp push pedal can carry out vertical movement relatively under drive assembly nine drives support riser, drive assembly ten is connected with drive mechanism ten and can drive under the drive of actuating mechanism ten, drive mechanism ten is connected with shakes even transfer seat, shake even transfer seat and shake even bottom plate and connect, and shake even transfer seat and shake even bottom plate relatively under drive of drive assembly ten and carry out horizontal migration, shake even transfer seat on be provided with shake even base, shake even base can round with shake even transfer seat's junction rotation. The driving mechanism seven, the driving mechanism eight, the driving mechanism nine and the driving mechanism ten are all preferably motors, the transmission assembly eight is a combination of belt wheels and a transmission belt, the transmission assembly seven, the transmission assembly nine and the transmission assembly ten are all of screw-nut pair structures, and the guide assemblies are matched at the positions of each transmission assembly to realize the stability of transmission. The clamping plate is arranged on the supporting vertical plate, the clamping plate can horizontally move relative to the supporting vertical plate, the elastic floating clamping blocks are arranged on the clamping plate, and the elastic floating clamping blocks can be contacted with the shaking base after being extruded; the shaking base is provided with a shaking cam plate, a shaking roller is arranged on a shaking base plate, the shaking cam plate can be contacted with the shaking roller to realize the rotation of the shaking base when the shaking transfer base horizontally moves, the shaking transfer base is provided with a shaking elastic connecting piece, and the shaking elastic connecting piece is connected with the shaking transfer base and the shaking cam plate simultaneously. The clamping jaw can be driven by the driving mechanism eight to transversely run along the guide assembly to take the sample tube out of the sample module and put the sample tube into the shaking base, the driving mechanism ten drives the shaking base and the sample tube to advance along the guide assembly, the shaking base is subjected to 90-degree swinging motion after the shaking cam plate is contacted with the shaking roller, the shaking base is rotated under the action of the shaking elastic connecting piece to restore to the original position when the shaking base is retracted, and the sample tube is driven to swing and shake repeatedly. After shaking, the shaking base returns to the initial position, the driving mechanism nine drives the clamping pushing plate to move downwards, so that the clamping plate and the elastic floating clamping block move forwards to clamp the sample tube, and then the clamping jaw moves up and down along the guide assembly under the driving of the driving mechanism seven to finish cap pulling and cap covering actions. The shaking elastic connecting piece is preferably a shaking spring, the shaking spring is used as a tensioning and return part, the elastic floating clamping block is preferably a spring floating clamping block, and the shaking base is clamped by the spring floating clamping block, so that the shaking base is fixed and cannot rotate.
Further, the incubation module comprises an incubation bottom plate connected with the base, a driving mechanism eleven and a transmission assembly eleven are arranged on the incubation bottom plate, the transmission assembly eleven is connected with the driving mechanism eleven and can be driven by the driving mechanism eleven to transmit, the driving mechanism eleven is connected with a lighting module, and the lighting module can horizontally move relative to the incubation bottom plate under the driving of the driving mechanism eleven; the lighting module comprises a driving mechanism twelve and a transmission assembly twelve, the transmission assembly twelve is connected with the driving mechanism twelve and can be driven by the driving mechanism twelve to transmit, the transmission assembly twelve is connected with the optical module, and the optical module can horizontally move relative to the incubation bottom plate under the driving of the transmission assembly twelve. The driving mechanism eleven and the driving mechanism twelve are both preferably motors, the driving mechanism eleven adopts the combination of belt wheels and a conveyor belt, the transmission assembly eleven adopts a screw nut pair structure, and the guide assembly is matched at each transmission assembly to realize the transmission stability. After the transport module transports the reagent card strips to the incubation module, the reagent card reacts with the reagent, and after the reagent card strips react, the optical module can realize transverse and longitudinal movement under the combined action of the driving mechanism eleven and the driving mechanism twelve, and finally lighting of the reagent card at different incubation positions is realized. The transport module transports the reagent card strip which completes lighting to the discharging position of the incubation bottom plate, and the driving mechanism eleven drives the optical module to push the waste card pushing plate (capable of elastically resetting) to push the reagent card into the waste card sliding groove, so that the reagent card falls along the inclined plane. Closed-loop control can be realized by matching with the optical code teeth so as to improve the lighting movement precision of the whole module, the temperature sensor can accurately detect the reaction temperature of the clamping strip, and the reaction temperature of the whole incubation module is accurately controlled by matching with the heating plate.
Further, the sample adding module comprises a sample adding transverse plate connected with the base, a driving mechanism thirteen and a transmission assembly thirteen are arranged on the sample adding transverse plate, the transmission assembly thirteen is connected with the driving mechanism thirteen and can be driven by the driving mechanism thirteen to transmit, the transmission assembly thirteen is connected with a sample adding longitudinal plate, and the sample adding longitudinal plate can horizontally move relative to the sample adding transverse plate under the driving of the transmission assembly thirteen; the sample adding longitudinal plate is provided with a driving mechanism fourteen and a transmission assembly fourteen, the transmission assembly fourteen is connected with the driving mechanism fourteen and can be driven by the driving mechanism fourteen to transmit, the transmission assembly fourteen is connected with a sample adding assembly, and the sample adding assembly can horizontally move relative to the sample adding longitudinal plate under the driving of the transmission assembly fourteen; the sample adding assembly comprises a driving mechanism fifteen and a transmission assembly fifteen, the transmission assembly fifteen is connected with the driving mechanism fifteen and can be driven by the driving mechanism fifteen to transmit, the transmission assembly fifteen is connected with a piston sample adding assembly, the piston sample adding assembly can vertically move relative to the sample adding longitudinal plate under the driving of the transmission assembly fifteen, and the piston sample adding assembly is connected with a gun head. The driving mechanism thirteen, the driving mechanism fourteen and the driving mechanism fifteen are all preferably motors, the transmission assembly thirteen and the transmission assembly fourteen are all combinations of belt wheels and conveyor belts, the transmission assembly fifteen adopts a screw-nut pair structure, and the guide assemblies are matched at the transmission assemblies of each set to realize the stability of transmission. The gun head can realize the movement of three dimensions of the gun head, namely, the movement to each preset position by the driving of three motors.
Further, the suction head box module comprises a movable bottom plate connected with the base, the movable bottom plate can horizontally move relative to the base, a diluent mounting plate, a suction head box mounting plate and a reaction box mounting plate are arranged on the movable bottom plate, and a TIP head unloading plate is arranged below the suction head box mounting plate. The movable bottom plate is provided with a handle, the suction head box module is pulled out manually through the handle to complete replacement of diluent, the suction head box set and the mixing reaction box, and after replacement, the suction head box set is pushed to a preset position to wait for the gun head to load the TIP head on the suction head box set, and the diluent and the sample are loaded into the mixing reaction box to be mixed uniformly. Automatic unloading of the gun heads is completed through the TIP head unloading plate and the gun heads are collected into the waste collection box.
Further, the reagent cartridge module comprises a cartridge supporting frame connected with the base, a cartridge partition board is arranged on the cartridge supporting frame, and a space for accommodating the reagent cartridge is reserved between the cartridge partition boards. The reagent cassettes are placed in the preset clamping groove positions, the clamping groove positions and the detection types of the cassettes are set in one-to-one correspondence through software, the corresponding cassettes are placed in the corresponding clamping groove positions, and the detection sensors are arranged to monitor the placement state of the reagent cassettes in real time. A hook notch is reserved in front of a card box coaming of the card box supporting frame, so that a transport module can conveniently hook reagents in the card box into the transport module.
In conclusion, compared with the prior art, the utility model has the following beneficial effects:
(1) The sample module is utilized to realize automatic sample in-out and sample information reading, so that the rapid detection and reaction of the product are more controllable, the detection sensitivity and precision are also greatly improved, and the manual labor intensity is also reduced;
(2) The shaking cap pulling module is utilized to realize the overturning and shaking of the test tube in the sample module, and the pulling and cap covering of the test tube cap;
(3) The automatic picking and transferring of the reagent card strips can be realized by utilizing the transport module;
(4) The automatic adding, removing and state detecting of the suction head are realized by utilizing a suction head box module;
(5) The incubation module is used for incubating and lighting the reagent strips, so that the movable and dynamic lighting can be realized;
(6) The lighting modularized design of this scheme, complete machine integration is more nimble fast.
Drawings
The accompanying drawings, which are included to provide a further understanding of embodiments of the utility model and are incorporated in and constitute a part of this application, illustrate embodiments of the utility model. In the drawings:
fig. 1 is a schematic diagram of the overall structure of the present utility model.
Fig. 2 is a schematic diagram of a sample block.
Fig. 3 is a schematic structural view of the cartridge module.
Fig. 4 is a schematic structural view of the transport module.
Fig. 5 is a schematic structural view of the shaking-up cap module.
Fig. 6 is a schematic structural view of the incubation module.
Fig. 7 is a schematic structural diagram of the control module.
FIG. 8 is a schematic diagram of the structure of the sample application module.
FIG. 9 is a schematic view of the structure of the tip cassette module.
The names corresponding to the reference numerals in the drawings are:
1-sample module, 2-reagent cartridge module, 3-transport module, 4-shaking cap module, 5-incubation module, 6-control module, 7-sample application module, 8-tip cartridge module, 9-drive mechanism I, 10-sensor I, 11-sample support, 12-guide component II, 13-emergency department, 14-scan rotating motor, 15-scan control board, 16-code scanning module, 17-sensor II, 18-drive component II, 19-drive mechanism II, 20-sample frame stopper, 21-sample frame post, 22-optocoupler I, 23-sample fork, 24-guide component III, 25-drive component III, 26-drive mechanism III, 27-drive board card I, a first 28-guide assembly, a first 29-transmission assembly, a 30-cartridge support riser, a 31-cartridge support base plate, a 32-cartridge shroud, a 33-reagent cartridge, a 34-cartridge baffle, a 35-cartridge detection sensor, a second 36-optocoupler, a fourth 37-transmission assembly, a fourth 38-guide assembly, a fourth 39-cable protection assembly, a sixth 40-drive mechanism, a 41-transport fork, a fifth 42-guide assembly, a fifth 43-drive mechanism, a fourth 44-drive mechanism, a 45-transport base plate, a 46-transport bin column, a fifth 47-transmission assembly, a sixth 48-guide assembly, a 49-transport bin, a 50-shaking base plate, a third 51-optocoupler, a fourth 52-guide column, a 53-clamping plate, a seventh 54-guide assembly, the device comprises a 55-driving mechanism ten, a 56-clamping push plate, a 57-optical coupler four, a 58-driving mechanism nine, a 59-reinforcing side plate, a 60-guiding component eight, a 61-driving component eight, a 62-transferring transverse plate, a 63-optical coupler five, a 64-driving mechanism seven, a 65-cable wiring groove, a 66-driving mechanism eight, a 67-clamping jaw, a 68-guiding component nine, a 69-supporting vertical plate, a 70-elastic floating clamping block, a 71-shaking base, a 72-shaking cam plate, a 73-shaking elastic connecting piece, a 74-shaking transfer seat, a 75-shaking roller, a 76-guiding component ten, a 77-clamping spring pressing piece, a 78-incubation bottom plate, a 79-temperature sensor, a 80-optical module and a 81-optical coupler six, 82-driving mechanism twelve, 83-cable protection component II, 84-guide component II, 85-guide component eleven, 86-optical code ruler, 87-driving mechanism eleven, 88-driving component eleven, 89-waste card push plate, 90-supporting column, 91-waste card chute, 92-heating plate, 93-mounting backboard, 94-driving board card II, 95-temperature control board, 96-driving board card III, 97-power supply adapter plate, 98-switching power supply, 99-loading supporting column, 100-driving component thirteen, 101-loading transverse plate, 102-driving mechanism thirteen, 103-guide component twelve, 104-optical coupling seven, 105-cable protection component III, 106-driving mechanism fourteen, 107-loading longitudinal plate, the device comprises a guide component, a cable protection component, a transmission component, a piston loading component, a drive component, a movable bottom plate, a diluent filling bottle, a diluent sucking box, a mixing reaction box, a waste collection box, a TIP unloading plate and a handle.
Description of the embodiments
For the purpose of making apparent the objects, technical solutions and advantages of the present utility model, the present utility model will be further described in detail with reference to the following examples and the accompanying drawings, wherein the exemplary embodiments of the present utility model and the descriptions thereof are for illustrating the present utility model only and are not to be construed as limiting the present utility model.
Examples
As shown in fig. 1, the embodiment includes a base, the base is a supporting device of the whole instrument, is a larger platform, and ensures stability of the instrument, and is provided with a sample module 1, a reagent cartridge module 2, a transport module 3, a shaking cap module 4, an incubation module 5, a control module 6, a sample adding module 7 and a suction head cartridge module 8, wherein the sample module 1, the transport module 3, the shaking cap module 4, the incubation module 5 and the sample adding module 7 are all connected with the control module 6; the transport module 3 can pick up the reagent strips in the reagent cartridge module 2; the shaking cap pulling module 4 can turn over the test tube in the sample module 1 and pull out and cover the test tube cap; the incubation module 5 can incubate and collect light for the reagent strips; the sample adding module 7 can absorb samples for sample adding. Through these structure combinations, make product short-term test and reaction more have the controllability, detection sensitivity and precision also have great promotion, also reduce artifical intensity of labour, this scheme daylighting modularized design, the complete machine integration is more quick nimble.
The working principle of this embodiment is as follows:
the method comprises the steps of putting reagent cartridges of different items into a reagent cartridge module 2 and setting the reagent cartridges to correspond to software, putting tip heads and diluent into a suction head cartridge module 8, putting a sample to be tested into a sample module 1, inputting sample test information to be tested into test software and clicking on the beginning test, hooking reagent strips of corresponding items by a transport module 3, transporting the reagent strips to a sample adding position, adding tip heads and absorbing diluent by a sample adding module 7, injecting diluent into corresponding reaction pits of a mixing reaction cartridge by the sample adding module 7, reading sample information by the sample module 1, carrying a sample tube into a shaking base by a shaking cap module 4, shaking the sample tube, taking a test tube cap, transporting the shaking base to a sample adding waiting position, discharging the sample into the corresponding pits of the mixing reaction cartridge by the sample adding module 7, sucking the mixed sample strips into a sample discharging port of the reagent strips by the transport module 3, transporting the strips into an incubation module 5, incubating the sample adding module 7, collecting the sample strips by the sample adding module 3, taking the sample strips, transporting the sample strips by the sample tube after the sample adding module, removing the sample tube cap module 7, removing the sample tube after the sample tube cap module is used for shaking the sample tube into the shaking base, removing the sample tube cap module, and removing the sample tube after the sample tube cap module is used for carrying the sample tube.
Examples
As shown in fig. 2, the sample module 1 of this embodiment includes a sample support 11, a bottom plate is further disposed below the sample support 11, the bottom plate is attached to and fixed with the base, the sample support 11 is connected with the bottom plate through a sample support post 21, so that an installation space is formed between the sample support 11 and the bottom plate, a first driving mechanism 9, a first driving mechanism 29, a second driving mechanism 19, a second driving mechanism 18, a third driving mechanism 26 and a third driving mechanism 25 are all disposed between the sample support 11 and the bottom plate, the first driving mechanism 29 is connected with the first driving mechanism 9 and can be driven by the first driving mechanism 9 to drive, the second driving mechanism 18 is connected with the second driving mechanism 19 and can be driven by the second driving mechanism 19 to drive, the third driving mechanism 25 is connected with the third driving mechanism 26 and can be driven by the third driving mechanism 26 to drive, the driving directions of the first driving mechanism 29 and the third driving mechanism 25 are the same, and the driving directions of the second driving mechanism 18 and the third driving mechanism 25 are perpendicular. A sample shifting fork 23 is arranged below the sample bracket 11, and the sample shifting fork 23 is connected with a transmission assembly III 25 and can horizontally move relative to the sample bracket 11 under the drive of the transmission assembly III 25. The bottom plate is provided with a first guide component 28 for limiting the moving track of the component connected with the first transmission component 29, a third guide component 24 is connected with the sample shifting fork 23 for limiting the moving track of the sample shifting fork 23, the top of the sample shifting fork 23 can be inserted into a corresponding slideway arranged at the bottom of the sample bracket 11, and the sample shifting fork 23 can move along the slideway. The extreme position of the sample fork 23 is provided with an optocoupler 22 for limiting the movement distance thereof. The driving mechanism III 26 drives the transmission assembly III 25 to move, so that the sample shifting fork 23 moves in the slideway, and the sample rack to be tested is pushed to move horizontally on the sample bracket 11. A second guide assembly 12 is arranged to limit the moving track of a part connected with a second transmission assembly 18, a sample frame limiting block 20 is arranged on the sample support 11 to limit the moving track of the sample frame, and a first sensor 10 and a second sensor 17 are arranged on the sample support 11 to realize position detection.
The outside of sample support 11 is provided with horizontal bottom plate, install scanning rotating electrical machines 14 on the horizontal bottom plate, scan control panel 15, sweep a yard module 16, scan rotating electrical machines 14 drive sweep a yard module 16 action, horizontal bottom plate and drive assembly two 18, guide assembly two 12 are connected, drive through actuating mechanism two 19 and carry out lateral shifting along guide assembly two 12, be connected with horizontal shift fork on the guide assembly two 12 simultaneously, when the sample frame that awaits measuring moves extreme position under actuating mechanism three effects, horizontal shift fork just with survey sample frame lock for it becomes the test sample frame, test sample frame carries out horizontal migration and carries out bar code information reading through scanning station relative sample support 11.
The first transmission component 29 is connected with a sample outlet shifting fork, the sample outlet shifting fork is positioned in a corresponding slideway arranged at the bottom of the sample support, and the sample outlet shifting fork can move along the slideway. The sample ejection fork is connected to the first guide assembly 28, and the sample ejection fork is movable along the first guide assembly 28. When the scanning is completed and then the sample rack is moved to a preset position, the sample outlet shifting fork hooks the test sample rack, the sample rack can horizontally move relative to the sample rack 11 under the action of the first driving mechanism 9, and meanwhile, the connection relation with the synchronous belt in the second driving assembly 18 is released, and the test sample rack becomes a tested sample rack along with the movement of the test sample rack. An emergency department 13 is reserved on the sample support 11, so that the application scene of the complete machine is further improved.
Placing a sample to be tested into a sample rack to be tested of the sample support 11, taking care that a bottom notch is required to be aligned with a sample rack limiting block 20 (fool-proof function) when the sample rack is placed, enabling a driving mechanism III 26 to act together with a transmission component III 25 and a sample feeding shifting fork to move the sample rack to a preset position, and arranging a sensor II 17 to detect the state of the sample rack; the second driving mechanism 19 and the second transmission assembly 18 act together to move the sample rack to a preset position, and the code scanning module 16 reads bar code information; after the sample is processed, the first driving mechanism 9, the first transmission component 29 and the sample discharging shifting fork act together to move the sample frame to a preset position, and the state of the sample frame is detected by the first sensor 10; and an emergency treatment position 13 is arranged in the sample device to increase the application environment of the matched instrument. The scheme can realize automatic movement of the sample rack; the automatic reading of sample information can be realized; the sample rack in-place detection can be realized; the emergency sample can be independently placed; multiple groups of sample frames can be placed simultaneously; the limit of the placing direction of the sample rack can be realized.
Examples
As shown in fig. 3, the reagent cartridge module 2 of the present embodiment includes a cartridge supporting frame connected to a base, a cartridge partition 34 is provided on the cartridge supporting frame, and a space for accommodating a reagent cartridge 33 is reserved between the cartridge partitions 34. The cartridge support frame comprises a cartridge support vertical plate 30, a cartridge support bottom plate 31 and a cartridge coaming 32, wherein the cartridge support vertical plate 30 is connected with a base, the cartridge support bottom plate 31 is fixed with the top of the cartridge support vertical plate 30, the cartridge coaming 32 surrounds the top of the cartridge support bottom plate 31 to form an internal hollow structure, the cartridge partition plate 34 is arranged in the hollow structure, the reagent cartridges 33 are placed in various preset clamping groove positions, the clamping groove positions and the cartridge detection types are in one-to-one correspondence through software setting, the corresponding clamping groove positions are placed in corresponding clamping boxes, and the placement state of the reagent cartridges 33 can be monitored in real time by the detection sensors 35. The front of the card box coaming 32 is provided with a hook notch, so that the transportation module can conveniently hook the reagent in the card box into the transportation module.
Examples
As shown in fig. 4, the transporting module 3 of this embodiment includes a transporting bottom plate 45, a driving mechanism four 44 and a driving component four 37, the driving mechanism four 44 and the driving component four 37 are all fixed on the base, the driving component four 37 and the driving mechanism four 44 are connected and can drive under the driving of the driving mechanism four 44, the transporting bottom plate 45 and the driving component four 37 are connected and can move horizontally relative to the base under the driving of the driving component four 37, the transporting bottom plate 45 moves and limits and guides through a guiding component four 38, the guiding component four 38 is installed on the base, and the transporting bottom plate 45 is further provided with an optical cable protection component one 39. The transport base 45 is provided with a driving mechanism five 43, a transmission assembly five 47, a driving mechanism six 40, a transmission assembly six, a transport shifting fork 41 and a transport bin 49, and the transport bin 49 is connected with the transport base 45 through a transport bin upright 46 to form a certain height. The transmission assembly five 47 and the driving mechanism five 43 are connected and can drive under the driving of the driving mechanism five 43, the transmission assembly six and the driving mechanism six 40 are connected with the transmission assembly five 47 as a whole and can horizontally move relative to the transport base plate 45 under the driving of the transmission assembly five 47, the transmission assembly six and the driving mechanism six 40 are connected and can drive under the driving of the driving mechanism six 40, the transport shifting fork 41 and the transmission assembly six are connected and can vertically move relative to the transport base plate 45 under the driving of the transmission assembly six and can be inserted into and pulled out of the transport bin 49, and the guide assembly five 42 is provided for moving limiting and guiding. The transporting fork 41 can realize the movement in the vertical direction under the drive of the driving mechanism six 40, can realize the longitudinal movement along the guiding component six 48 under the drive of the driving mechanism five, and can realize the transverse movement along the guiding component four 38 under the drive of the driving mechanism four 44. Finally, the reagent card can be transported to and removed from the respective predetermined position of the incubation module and placed in the transport magazine 49. The sensor and the second optical coupler 36 are used for monitoring the state of the reagent card, and the code scale is used for realizing closed-loop control to improve the movement precision of the whole module.
Examples
As shown in fig. 5, the shaking cap module 4 of this embodiment includes a shaking base plate 50 fixed to the base, a supporting vertical plate 69 is vertically fixed above the shaking base plate 50, and a supporting reinforcing side plate 59 is further provided on a side portion of the supporting vertical plate 69 to increase structural stability for increasing stability. The cable wiring groove 65 is arranged on the supporting vertical plate 69, the driving mechanism seven 64 and the transmission assembly seven are arranged on the supporting vertical plate 69, the transmission assembly seven is connected with the driving mechanism seven 64 and can be driven by the driving mechanism seven 64 to transmit, the transmission assembly seven is connected with the transfer transverse plate 62, and the transfer transverse plate 62 can vertically move relative to the supporting vertical plate 69 under the driving of the transmission assembly seven; the transfer transverse plate 62 is provided with a driving mechanism eight 66 and a transmission assembly eight 61, the transmission assembly eight 61 is connected with the driving mechanism eight 66 and can be driven by the driving mechanism eight 66 to transmit, the transmission assembly eight 61 is connected with a clamping jaw 67, and the clamping jaw 67 can horizontally move relative to the transfer transverse plate 62 under the driving of the transmission assembly eight 61; the support riser 69 is provided with a driving mechanism nine 58, a transmission assembly nine, a driving mechanism ten 55 and a transmission assembly ten, the transmission assembly nine is connected with the driving mechanism nine 58 and can drive under the driving of the driving mechanism nine 58, the transmission assembly nine is connected with the clamping push plate 56, the clamping push plate 56 can vertically move relative to the support riser 69 under the driving of the transmission assembly nine, the clamping push plate 56 is connected with a guide assembly seven 54, the guide assembly seven 54 is fixed with the support riser 69, and the guide and limiting of the clamping push plate 56 are realized. The transmission assembly is ten and the drive mechanism is ten 55 are connected and can drive under the drive mechanism is ten 55, the drive mechanism is ten 55 to be connected with and shake even transfer seat 74, shake even transfer seat 74 and shake even bottom plate 50 and be connected, be provided with guide assembly ten 76 on the even bottom plate 50, guide assembly ten 76 and shake even transfer seat 74 and be connected, and shake even transfer seat 74 and can shake even bottom plate 50 relatively under the drive of transmission assembly ten and carry out horizontal migration, shake even transfer seat 74 and be provided with and shake even base 71, shake even transfer seat 74 and shake even base 71 and pass through same round pin hub connection, shake even base 71 and can rotate round the axis of round pin axle.
The supporting vertical plate 69 is provided with the clamping plate 53, the clamping plate 53 can horizontally move relative to the supporting vertical plate 69, the clamping plate 53 is provided with the first guide column 52, and the first guide column 52 penetrates through the clamping plate 53 to be fixed with the supporting vertical plate 69, so that the guiding and limiting functions are achieved. The clamping plate 53 is provided with an elastic floating clamping block 70, and the elastic floating clamping block 70 can be clamped with the shaking base 71 after being extruded; the shaking base 71 is provided with a shaking cam plate 72, the shaking base plate 50 is provided with a shaking roller 75, the shaking cam plate 72 can be contacted with the shaking roller 75 to realize the rotation of the shaking base 71 when the shaking transfer base 74 horizontally moves, the shaking transfer base 74 is provided with a shaking elastic connecting piece 73, and the shaking elastic connecting piece 73 is connected with the shaking transfer base 74 and the shaking cam plate 72 at the same time.
The electric clamping jaw 67 can transversely run along the guide assembly eight 60 under the drive of the drive mechanism eight 66 to take a sample tube out of the sample module and put the sample tube into the shaking base 71, the drive mechanism ten 55 drives the shaking base 71 and the sample tube to advance along the guide assembly ten 76, the shaking base 71 is subjected to 90-degree swinging motion after the shaking cam plate 72 is contacted with the shaking roller 75, and the shaking base 71 is rotated to restore to the original position under the action of the shaking elastic connecting piece 73 when the shaking base 71 is retracted, so that the sample tube is repeatedly driven to swing and shake. After shaking up, the shaking up base 71 returns to the initial position, the driving mechanism nine 58 is used as a clamping driving mechanism to drive the clamping pushing plate 56 to move downwards, so that the clamping plate 53 and the elastic floating clamping block 70 move forwards to clamp the sample tube, and then the electric clamping jaw 67 moves up and down along the guide assembly nine 68 under the driving of the driving mechanism seven 64 to complete cap pulling and cap covering actions. An optical coupler III 51, an optical coupler IV 54 and an optical coupler V63 are respectively arranged to detect the moving position.
Examples
As shown in fig. 6, the incubation module 5 of this embodiment includes an incubation bottom plate 78 connected with a base, the incubation bottom plate 78 is fixed with the base through a support post 90, a clamping spring pressing piece 77 is provided on the incubation bottom plate 78 to mount a reagent clamping strip, and a second cable protection assembly 83 is provided on the incubation bottom plate 78. Be provided with actuating mechanism eleven 87 and drive assembly eleven 88 on incubating the bottom plate 78, drive assembly eleven 88 and actuating mechanism eleven 87 are connected and can drive under actuating mechanism eleven 87 drive, and actuating mechanism eleven 87 is connected with the daylighting module, and the daylighting module can be under actuating mechanism eleven 87 drive relative incubation bottom plate 78 horizontal migration, is provided with six 81 optocouplers on the daylighting module and carries out position detection, and install guiding assembly eleven 85 and daylighting module connection on incubating the bottom plate 78 and inject the removal orbit. The lighting module comprises a driving mechanism twelve 82 and a transmission assembly twelve, the transmission assembly twelve is connected with the driving mechanism twelve 82 and can be driven by the driving mechanism twelve 82 to transmit, the transmission assembly twelve is connected with the optical module 80, and the optical module 80 can horizontally move relative to the incubation bottom plate 78 under the driving of the transmission assembly twelve. The lighting module is also provided with a second guide piece 84 for realizing movement guiding and limiting.
After the transport module 3 transports the reagent card strip to the incubation module 5, the reagent card reacts with the reagent first, and after the reagent card reacts, the lighting module can realize transverse and longitudinal movement under the combined action of the driving mechanism eleven and the driving mechanism twelve, and finally lighting of the reagent card at different incubation positions is realized through the optical module 80. The transport module 3 transports the reagent card strip which completes lighting to the discharging position of the incubation bottom plate 78, and the driving mechanism eleven drives the optical module 80 to push the waste card pushing plate 89 (capable of elastically resetting) to push the reagent card into the waste card sliding groove 91, so that the reagent card falls along the inclined plane. Closed-loop control can be realized by matching with the optical code teeth 86 to improve the lighting movement precision of the whole module, the temperature sensor 79 can accurately detect the reaction temperature of the clamping strip, and the heating plate 92 is matched to accurately control the reaction temperature of the whole incubation module.
Examples
As shown in fig. 7, the control module 6 of the present embodiment includes a mounting backboard 93, and the mounting backboard 93 is provided with a second driving board card 94, a temperature control board 95, a third driving board card 96, a power supply adapter board 97 for a printer, and a switching power supply 98, which are all existing structures and can be directly purchased.
Examples
As shown in fig. 8, the sample loading module 7 of this embodiment includes a sample loading cross plate 101, and the sample loading cross plate 101 is connected to the base through a sample loading support column 99, and a waste cartridge baffle 114 is mounted on the sample loading support column 99. The cable protection assembly III 105, the optocoupler seven 104, the driving mechanism thirteen 102 and the transmission assembly thirteen 100 are arranged on the sample loading transverse plate 101, the transmission assembly thirteen 100 is connected with the driving mechanism thirteen 102 and can be driven by the driving mechanism thirteen 102 to transmit, the transmission assembly thirteen 100 is connected with the sample loading longitudinal plate 107, the sample loading longitudinal plate 107 can horizontally move relative to the sample loading transverse plate 101 under the driving of the transmission assembly thirteen 100, the guide assembly twelve 103 is arranged on the sample loading transverse plate 101, and the sample loading longitudinal plate 107 is connected with the guide assembly twelve 103 and realizes the movement guide limit. Be provided with cable protection subassembly IV 109, actuating mechanism fourteen 106 and drive assembly fourteen 110 on the application of sample vertical plate 107, drive assembly fourteen 110 and drive mechanism fourteen 106 are connected and can drive under the drive of actuating mechanism fourteen 106, drive assembly fourteen 110 is connected with the application of sample subassembly, the application of sample subassembly can drive under drive assembly fourteen 110 relative application of sample vertical plate 107 horizontal migration, be provided with the thirteenth 108 of guide assembly on the application of sample vertical plate 107, the thirteenth 108 of guide assembly is connected with the application of sample subassembly and realizes moving the direction spacing. The sample adding assembly comprises a driving mechanism fifteen 113 and a transmission assembly fifteen, the transmission assembly fifteen is connected with the driving mechanism fifteen 113 and can be driven by the driving mechanism fifteen 113 to transmit, the transmission assembly fifteen is connected with a piston sample adding assembly 111, the piston sample adding assembly 111 can vertically move relative to the sample adding longitudinal plate under the driving of the transmission assembly fifteen, and the piston sample adding assembly 111 is connected with a gun head. The guide assembly fourteen 112 is arranged in the sample adding assembly, and the guide assembly fourteen 112 is connected with the piston sample adding assembly 111 to realize movement guide limiting.
The movement of the drive mechanism thirteen 102 can realize the lateral movement of the piston loading assembly 111 (including but not limited to a plunger pump) on the guide assembly twelve 103; the motion of the drive mechanism fourteen 106 may enable longitudinal movement of the piston loading assembly 111 (including but not limited to a plunger pump) on the guide assembly thirteenth 108; the piston sample adding component 111 (including but not limited to a plunger pump) can realize the vertical movement of the front end gun head under the drive of the driving mechanism fifteen 113, and can realize the movement of the gun head in three dimensions, namely to each preset position by matching with the transverse direction and the longitudinal direction.
Examples
As shown in fig. 9, the tip box module 8 of the present embodiment includes a movable bottom plate 116 connected to the base, and the movable bottom plate 116 can move horizontally with respect to the base, and is guided and limited by a chute guide 115. The movable bottom plate 116 is provided with a diluent mounting plate, a suction head box mounting plate and a reaction box mounting plate, a diluent filling bottle 117 is mounted on the diluent mounting plate, a suction head box sleeve 118 is mounted on the suction head box mounting plate, a uniformly mixed reaction box 119 is mounted on the reaction box mounting plate, a TIP head unloading plate 121 is arranged below the suction head box mounting plate, and a waste collecting box 120 is arranged below the TIP head unloading plate 121. The replacement of the diluent, the TIP box set and the mixing reaction box is completed by manually pulling out the TIP box module through the handle 122, and the TIP box set 118 is pushed to a preset position to wait for the TIP after the replacement, and the diluent and the sample are loaded into the mixing reaction box 119 for mixing, etc. Automatic unloading of the gun heads is accomplished by the TIP head unloading plate 121 and collection into the waste collection box 120.
The foregoing detailed description of the utility model has been presented for purposes of illustration and description, and it should be understood that the utility model is not limited to the particular embodiments disclosed, but is intended to cover all modifications, equivalents, alternatives, and improvements within the spirit and principles of the utility model.