CN104297498B - A kind of full-automatic biochemical and luminescence immunoassay system - Google Patents
A kind of full-automatic biochemical and luminescence immunoassay system Download PDFInfo
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- CN104297498B CN104297498B CN201310312544.7A CN201310312544A CN104297498B CN 104297498 B CN104297498 B CN 104297498B CN 201310312544 A CN201310312544 A CN 201310312544A CN 104297498 B CN104297498 B CN 104297498B
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Abstract
The invention discloses a kind of full-automatic biochemical and luminescence immunoassay system, comprise large mechanical arm, sample-adding pump, the sample needle that can carry out XYZ motion, scouring stage, flushing pump, drawing liquid pump, agent bin, sample storehouse, reaction rotating disk, incubation dish, vibration mix assembly, micromachine arm, cleaning station assembly, scavenging pump, waste drains pump, measurement darkroom assembly, excite reagent pump, excite reagent needles, photon survey assembly, light source assembly, spectral measurement assembly, discarded reaction cup passage, reaction cup automatic loading assembly, control circuit and computer. Biochemical indicator is detected this instrument and luminescence immunoassay is integrated on same instrument, both realized the function of biochemical immunity streamline, greatly reduce again complexity and the cost of system, make infirmary also can carry out Full-automatic quantitative or qualitative detection to the biochemistry in blood of human body and other liquid and harmful additive, the medicament residue etc. in immune indexes, medicine concentration, microbial toxin and food.
Description
Technical field
The present patent application relates to a kind of full-automatic biochemical and luminescence immunoassay system, and this system can be rightBiochemistry and immune indexes carry out Full-automatic quantitative or qualitative detection, belong to biological monitoring and clinical examinationEquipment technical field.
Background technology
Automatic clinical chemistry analyzer is mainly used in biochemical and chemical in clinical blood, other humoral sampleComposition is as hemoglobin, cholesterol, triglycerides, albumin, c reactive protein, glutamic acid ammoniaThe quantitative assay of based transferase, glucose, potassium, sodium, calcium etc. is the most frequently used inspection of clinical analysisTest one of instrument. At present, what clinical treatment mechanism was generally used is discrete Biochemical Analyzer, instrumentDevice is filled by sample disc, reagent rack, sample pipetting volume device, reagent sample adding device, reaction tray, colorimetricPut, reaction cup cleaning device, agitating device, ISE assembly (apolegamy) and operating system composition.Immunological assay method is numerous, and at present, the measuring principle that automatic lmunoassays analyzer adopts mainly containsImmunoturbidimetry, time-resolved fluorescence, fluorescence polarization, collaurum, enzyme immunity and chemoluminescence method.Wherein, immunoturbidimetry sensitivity is lower, only for measuring c reactive protein, complement C3/C4 etc.The material that in-vivo content is higher; Time-resolved fluorescence method sensitivity is higher, reagent is more stable, as sweet smellThe full-automatic time resolved fluoro-immunoassay system of blue PerkinE1mer, but this method metering systemComplicated (pulse excitation, batch (-type) measurement, flow cell), instrument cost and maintenance cost are high, environmentAnd can cause background to disturb with dvielement in sample; Fluorescence polarization method is homogeneous assay method, precision is high,Analysis speed is not affected by sample of color and cloudy state soon,, as the TDx series of U.S. AbbottFluorescence polarization assay instrument, but this method is only confined to the antigen that determining molecular weight is less than 160kD at present,And sensitiveness not high (conventionally between 0.1-10ng); Colloidal gold method has higher sensitivity and spyThe opposite sex, detection time is short, only needs about 15 minutes, and testing process is simple, but conventionally can only orderSurvey qualitative interpretation, affected greatly by subjective factor, and cost is high; Chemoluminescence method, particularly tubular typeChemoluminescence method is highly sensitive, high specificity, easy and simple to handle, stable reagent, non-environmental-pollution,In immunology detection, obtain application very widely, Just because of this, national high-tech research is sent outGreat of exhibition plan (863 Program) biology and the product development of medical technical field vitro diagnostic techniquesOne of order problem is: the development of open type full automatic tube-type chemical electrochemiluminescent immunoassay detection system.In clinical practice, a patient often needs to carry out biochemistry and immune detection simultaneously, general at presentWay be that patient's sample is delivered to respectively and done biochemical analysis and doing on the instrument of immunoassay, noOnly need two large-scale equipment, and blood sampling volume is also many, brings more misery to patient, rightIn the bad patient of condition, too much blood sampling is also very unfavorable to patient, detects also and wants simultaneouslySpend the more time, when serious, can affect the diagnosis to patient.
Based on above-mentioned factor, people attempt biochemistry and immune detection to be integrated together just, form rawChange and immunoassay all-in-one, patent CN2847292Y discloses a kind of Full-automatic biochemical immunity analysisInstrument, both can carry out separately wet method biochemical analysis or immunoassay, can carry out again simultaneously wet method biochemical andImmunoassay, the VITROs5600 Full-automatic biochemical immunity analysis instrument of Ortho company is to useVITROs reagent carries out dry chemical and immunoassays to sample. Above-mentioned automation equipment is examined to laboratorySurvey provides very big facility, but still there is more deficiency in actual use, problem or limitation,The most important thing is that the immunological detection method that they adopt is all colorimetric method, sensitivity is well below changeLearn chemiluminescence immunoassay method, can not meet clinical actual needs.
For biochemical analysis and luminescence immunoassay are organically combined, fewer companies pushes away both at home and abroadGo out biochemical immunity streamline, as Beckman biochemical immunity streamline, Siemens's biochemical immunity flowing waterLine, the IBE6000 biochemical immunity streamline of the new industry in Shenzhen, its essence is two full-automatic pointAnalysing instrument (automatic clinical chemistry analyzer and chemiluminescent analyzer) is connected by sample tube induction systemTogether, whole system complexity, costliness, is only applied in the hospital that minority is large at present, is not easy to generalAnd.
Summary of the invention
Technical problem to be solved by this invention is to overcome above shortcomings in prior art,A kind of easy to operate, applied widely, the full-automatic biochemical that can realize biochemistry and immunoassay is providedAnd luminescence immunoassay system.
For convenience of describing, by abstract whole frame be rectangular plane, be horizontal along long side direction,Be longitudinally along short side direction, in addition, by lateral set be x axle, be longitudinally set as Y-axis, along machineFrame vertical direction is set as z axle, it should be explicitly made clear at this point, this address is for convenience of description,And be not the restriction to structure.
A kind of full-automatic biochemical described in the present patent application and luminescence immunoassay system, comprise and can carry outLarge mechanical arm, sample-adding pump, the sample needle of XYZ motion, scouring stage, flushing pump, drawing liquid pump, examinationAgent storehouse, sample storehouse, reaction rotating disk, incubation dish, vibration mix assembly, micromachine arm, cleaningStand assembly, scavenging pump, waste drains pump, measurement darkroom assembly, excite reagent pump, excite reagent needles,Photon survey assembly, light source assembly, spectral measurement assembly, discarded reaction cup passage, reaction cup are certainlyMoving loading assembly, control circuit and computer.
Large mechanical arm can side-to-side movement in X-axis, and prop up the left and right that X-axis is mounted in system backplaneOn frame, on large mechanical arm, Y-axis motion and Z axis motion are installed, move at Z axisSample needle is installed in mechanism, and sample needle is connected with sample-adding pump by pipeline, thereby by large machineryThe motion of the XYZ axle of arm, is added to reaction cup by the sample in the reagent in kit and sample tubeIn.
Large mechanical arm can only have an arm, and sample and reagent all add by the same sample needle on armSample, also can be divided into left arm and right arm, and one of them arm is responsible for adding sample, and another one arm is responsible forReagent adding, reduces cross pollution, and preferred version of the present invention is two large mechanical arms.
Agent bin, incubation dish are installed on the base plate of system, above incubation dish, are coaxially installed withReaction rotating disk, rotating disk can rotate around axis, on rotating disk, be evenly processed with multiple reaction cup grooves forPlacing response cup (as shown in patent ZL201120411627.8); Reaction cup slot part on rotating diskIn incubation dish, to the reaction cup of putting into groove is heated.
On reaction rotating disk, bottom and the side of each glass of groove has through hole, the through hole of bottom both forThe existence of detection reaction cup, again for mixing reactant in reaction cup by vibration mixing mechanismEven, also help simultaneously reactant in reaction cup is heated, the through hole of side is in order to give birth toWhen fractional analysis, allow incident light see through the sample of reaction cup, realize spectral measurement.
Sample storehouse can be divided into basic storehouse and expansion storehouse, and coaxial installation the in basic storehouse is placed in reaction rotating diskSide, expansion storehouse is arranged on another routine base plate of reaction rotating disk, and system can have basic storehouse simultaneouslyWith expansion storehouse, also can only have basic storehouse or expansion storehouse.
Vibration mixes assembly by push rod, oscillator, top rod driving installation composition, and its oscillator is connected with push rod,Vibration mixes assembly and is arranged on the below of incubation dish, on the incubation dish above push rod, has through hole,Push rod can push up through the through hole of through hole thus and reaction rotating disk bottom the bottom of reaction cup, oscillatorDrive reaction cup vibration by push rod, the reactant in reaction cup is played to the effect mixing; Push rodThe effect of driving mechanism is in the time that needs mix, to make push rod rise to contact with reaction cup, does not need to mixTime make push rod in low level, do not hinder reaction dial rotation.
Cleaning station assembly is arranged on the outside of reaction rotating disk, in one end of cleaning station assembly, measurement is installedDarkroom assembly, is provided with photon survey assembly in one end of measuring darkroom assembly.
Cleaning station assembly separates by reacting cup, reaction cup traversing carriage, reaction cup lifting support, magneticBody, cleaning needle support, cleaning needle group, waste liquid pin group, useless cup groove composition, if having on reaction cupDry cup groove, is provided with magnetic chorista, cleaning station reaction cup lifting support and cleaning in part cup grooveThe reaction cup of standing traversing carriage is all arranged on reaction cup, and lifting support is placed on again on traversing carriage,Can make lifting support lifting on traversing carriage by a motion, another motion canThe position that drives traversing carriage, lifting support to move a reaction cup groove.
Measure darkroom assembly by measuring darkroom pedestal, measure darkroom drawer guideway, measure darkroom drawer,Shadow shield, speculum composition, on drawer, have can placing response cup cup groove, deviate from cup grooveThe one side of photon survey assembly is placed with speculum, and above pedestal, be provided with and excite reagent needles,In the time that drawer is closed, shadow shield is opened, and the cup groove on drawer is just in time positioned at the spy of photon survey assemblySurvey before device, excite reagent needles to be also just in time positioned at a glass appropriate location for groove top, make to excite the reagent canBe expelled to the reaction cup in glass groove. In the time that drawer is closed, shadow shield is also closed, protection photon surveyThe detector of assembly is not subject to the irradiation of exterior light.
Photon survey assembly is made up of photodetector, high-tension circuit, bleeder circuit, amplifying circuit.
Light source assembly comprises bulb, radiator, condenser;
Spectral measurement assembly is made up of condenser, slit, beam splitting system, photodetector.
Reaction cup automatic loading assembly is made up of reaction cup collating unit, slideway, funnel.
Micromachine arm is arranged on reaction rotating disk, cleaning station assembly and measures between the assembly of darkroom, and it turnsRotation axis rises on rotating disk on the distance of glass position and reaction cup cup in cleaning station assembly each to reactionThe distance of individual cup groove, useless cup groove and with to measure cup groove on the drawer of darkroom under open modeDistance is equal, and micromachine arm can pass between darkroom at reaction rotating disk and cleaning station, measurement like thisSend reaction cup. Play cup position and refer to that micromachine arm can be from the rotating disk cup groove inner clip in this positionThe cup that reacts, it is centered close to the center of rotation of micromachine arm to the line of reaction tray center of rotationOn.
Discarded reaction cup passage is arranged in the below of the useless cup of cleaning station assembly groove, and the bottom of useless cup groove is anti-Answer cup traversing carriage, on it, have a through hole near the position of useless cup groove, mobile response cup moves and props upFrame, can make this through hole move on to the below of useless cup groove, makes discarded reaction cup fall into discarded reaction cupPassage, thus discarded reaction cup is drawn outside instrument.
In the electrochemiluminescent immunoassay testing process of magnetic unpack format, product is cleaned to the mistake separatingJourney is implemented by cleaning station assembly. On the reaction cup of cleaning station, there is several glass of groove, canPlace several reaction cup. Cleaning station has magnetic chorista to realize magnetic to separate, and cleaning station has and movesThe moving function that is put into the reaction cup on cleaning station reaction cup, mobile response cup, makes it one by oneSuccessively by magnetic separated region and cleaning area. In cleaning area, scavenging pump is by cleaning on supportCleaning needle group reaction cup is cleaned, waste liquid is taken away by waste drains pump and waste liquid pin group. CleanBi Hou, then by micromachine arm, reaction cup is moved to the drawer in measurement darkroom in an open positionIn, closing drawers, measures darkroom sealed, now can be by exciting reagent pump assembly to exciteReagent joins in reaction cup, starts chemiluminescent reaction, and the light of institute's output is by photon survey listUnit measures, and the result of measurement is sent into computer and processed. After measurement finishes, measuring chamber is taken outDrawer is opened, by micromachine arm, reaction cup moved in the useless cup groove on cleaning station reaction cup,Waste liquid pin is taken waste liquid away. The bottom of useless cup groove is reaction cup traversing carriage, a close useless cup groove on itPosition have a through hole, mobile response cup traversing carriage, makes this through hole move on to the below of useless cup groove,Make this discarded reaction cup fall into discarded reaction cup passage. For some reagent, test request adoptsTwo-step method or three-step approach, after cleaning finishes, micromachine arm is not that reaction cup is sent into and measured secretlyChamber, but again send reaction rotating disk by reaction cup, then carry out the process of application of sample, incubation.
If testing process is biochemical analysis, when reagent, sample all add after reaction cup, through one sectionTime incubation, can carry out colorimetric measurement. In order to implement this measuring process, at one of incubation dishIndividual ad-hoc location (being called measuring position) has a through hole, in the both sides of through hole, light source can be installedAssembly and spectrographic detection assembly. If illuminating source is installed in a side of through hole, opposite side is installed and is dividedPhotosystem and detector, now the color solution body in reaction cup is positioned between light source and beam splitting system,I.e. so-called rear point optical mode; Also light source and beam splitting system can be placed on to the same example of reaction cup, thisIt is so-called front point optical mode. Because the front point optical mode of rear point of optical mode is superior, of the present invention excellentSelecting scheme is point optical mode after adopting. When colorimetric measurement by pending colorimetric measurement in reaction rotating diskReaction cup forwards the measuring position of incubation dish to, and the light that light source sends absorbs laggard through liquid selectiveEnter beam splitting system, photodetector can carry out photometric measurement to the light of beam splitting system outgoing, therebyCarry out biochemical colorimetric analysis. After finishing, colorimetric, by micromachine arm, reaction cup is moved to cleaning station anti-Answer in the useless cup groove on cup, waste liquid pin is taken waste liquid away, then this discarded reaction cup is fallen intoDiscarded reaction cup passage.
A kind of full-automatic biochemical described in the present patent application and luminescence immunoassay system, have excellent as followsPoint and beneficial effect:
This invention completes biochemical and electrochemiluminescent immunoassay detection on same machine, and instrument cost is only equivalent toThe cost of an instrument, well below the streamline of the configuration of instrument by two platform independent, is conducive to thisSystem is universal to middle and small hospital;
Because the reaction cup volume that the present invention adopts is small and exquisite, low price, is suitable for using one-time reactionCup, has avoided using and has cleaned the cross pollution that sordid reusable reaction cup causes, withAnd reuse reaction cup transmitance and reduce the impact on measurement result, and save whole complexityThe device of cleaning reaction cup, in application process, also save a large amount of cleaning fluids, reduced useCost, that has also avoided that a large amount of cleaning fluids causes environment may endanger.
Blood vessel does not need to be further divided into biochemical blood vessel and immune blood vessel, and blood drawing amount and blood vessel number reduce, and have alleviatedPatient's misery;
Patient's biochemistry, immune detection Turnaround Time are shortened greatly, improved operating efficiency and workQuality, is conducive to diagnose in time conditions of patients;
Reduce the chance that staff directly contacts sample, avoided cross pollution, improved experimentChamber bio-safety.
Brief description of the drawings
Fig. 1 is that full-automatic biochemical and luminescence immunoassay main structure of system show described in the present patent applicationIntention;
Fig. 2 is full-automatic biochemical and luminescence immunoassay system response rotating disk described in the present patent applicationStructural representation
Fig. 3 is the knot of full-automatic biochemical and luminescence immunoassay system cleaning station described in the present patent applicationStructure schematic diagram
Fig. 4 is full-automatic biochemical and luminescence immunoassay systematic survey darkroom described in the present patent applicationStructural representation;
In the drawings: large mechanical arm 1, sample-adding pump 2, sample needle 3, vibration mix assembly 4, rinseStand 5, flushing pump 6, drawing liquid pump 7, agent bin 8, basic sample storehouse 9, expansion sample storehouse 10,Reaction rotating disk 11, reaction rotating disk bottom through-hole 11-1, reaction rotating disk side through hole 11-2, incubationDish 12, cleaning station assembly 13, cleaning station reaction cup 13-1, magnetic chorista 13-2, cleaning stationReaction cup traversing carriage 13-3, cleaning station reaction cup lifting support 13-4, cleaning needle support 13-5,Cleaning needle group 13-6, waste liquid pin group 13-7, a useless cup groove 13-8, scavenging pump 14, waste drains pump 15,Micromachine arm 16, measurement darkroom assembly 17, measurement darkroom pedestal 17-1, measurement darkroom drawerGuide rail 17-2, measure darkroom drawer 17-3, shadow shield 17-4, speculum 17-5, excite reagentPump 18, excite reagent needles 19, photon survey assembly 20, discarded reaction cup passage 21, reaction cupCollating unit 22-1, slideway 22-2, funnel 22-3, light source assembly 23, spectral measurement assembly24, control circuit 25, computer 26.
Detailed description of the invention
Below in conjunction with accompanying drawing, the invention will be further described. Fig. 1 shown of the present invention one concreteEmbodiment, comprises that large mechanical arm 1, sample-adding pump 2, sample needle 3, vibration mix assembly 4, rinseStand 5, flushing pump 6, drawing liquid pump 7, agent bin 8, basic sample storehouse 9, expansion sample storehouse 10,Reaction rotating disk 11, incubation dish 12, cleaning station assembly 13, cleaning station reaction cup 13-1, magnetic divideIn vitro 13-2, cleaning station reaction cup traversing carriage 13-3, cleaning station reaction cup lifting support 13-4,Cleaning needle support 13-5, cleaning needle group 13-6, waste liquid pin group 13-7, useless cup groove 13-8, cleaningPump 14, waste drains pump 15, micromachine arm 16, measure darkroom assembly 17, measure darkroom pedestal 17-1,Measure darkroom drawer guideway 17-2, measure darkroom drawer 17-3, shadow shield 17-4, speculum 17-5,Excite reagent pump 18, excite reagent needles 19, photon survey assembly 20, discarded reaction cup passage 21,Reaction cup collating unit 22-1, slideway 22-2, funnel 22-3, light source assembly 23, spectral measurementAssembly 24, control circuit 25, computer 26. When measurement by automatic loading assembly by transparentReaction cup is loaded in rotating disk 11, manually kit is put into agent bin 8, and sample tube is putEnter in basic sample storehouse 9 or expansion sample storehouse 10. By XYZ motion and the application of sample of large mechanical armPump 2, sample needle 3 is added to the sample in the reagent in kit and sample tube in reaction cup,After application of sample completes, the reaction cup that has just added sample is forwarded to vibration and mix place, mix assembly 4 by vibrationTop rod driving device push rod is risen contact with reaction cup, and make reaction cup leave bottom rotating disk oneIndividual small distance, now to oscillator energising, makes oscillator produce vibration, thereby drives reaction cup to shakeMoving, its inner reactant is mixed. Mix after end, push rod is fallen, reaction cup fallsTo the bottom of rotating disk, in the incubation dish 12 that can keep constant temperature, carry out incubation. When certain reaction cupIncubative time arrives after official hour, is also to carry out biochemical analysis according to reactant in this reaction cupBe luminescence immunoassay, forward this reaction cup to different position.
If what carry out is that electrochemiluminescent immunoassay detects, this reaction cup is turned to cup position; By micro-Type mechanical arm 16 is moved on the reaction cup 13-1 of cleaning station. The lifting of cleaning station reaction cupSupport 13-4 and cleaning station reaction cup traversing carriage 13-3 are arranged on cleaning station reaction cup 13-1On. It is upper that lifting support 13-4 is placed on again traversing carriage 13-3, can make by a motionLifting support 13-4 lifting on traversing carriage 13-3, lifting support 13-4 drives and puts in the time risingAll reaction cup on reaction cup 13-1 rise together, until the bottom of reaction cup exceeds reactionThe groove plane of cup 13-1, now drives traversing carriage 13-3, lifting by another motionOn support 13-4 and lifting support, all reaction cup move the position of a reaction cup groove, then fallLower lifting support 13-4, drops in new cup groove all reaction cup above it, this luckActuation mechanism drives traversing carriage 13-3 and lifting support 13-4 to get back to original position again, prepares nextInferior circulation. Like this, cleaning station just has the mobile reaction cup on cleaning station reaction cup of being put intoFunction, mobile response cup one by one, and it is separated and cleaning area by magnetic successively. ClearlyThe magnetic of washing station separates by magnetic chorista 13-2 to be realized, and magnetic chorista 13-2 is arranged on reactionIn the cup groove of cup 13-1, can be native magnet, can be also electromagnet. In cleaning area,Scavenging pump 14 cleans reaction cup by the cleaning needle group 13-6 on cleaning needle support 13-5,Waste liquid is taken away by waste drains pump 15 and waste liquid pin group 13-7. After cleaning, if one-step method,By micromachine arm 16, reaction cup is moved in the cup groove of measuring darkroom drawer 17-3. CloseDrawer, measures darkroom sealed, measures shadow shield 17-4 in darkroom simultaneously and is opened, reaction cup positionBefore the photodetector of photon survey assembly, by measuring the reagent that excites on the pedestal 17-1 of darkroomPin 19, can program according to the rules excite reagent by exciting reagent pump 18 to add, and starts reactionThe chemiluminescence reaction of reactant in cup, the light of institute's output is measured by photon survey assembly 20,The result of measuring is sent into computer 26 and is processed. In order better to collect luminous energy, measuring darkroomThe one side that deviates from photon survey assembly in drawer 17-3 is placed with speculum 17-5, photon dorsadThe exciting light of measuring unit is reflected back photon measuring unit. After measurement finishes, drawer is opened, by micro-Type mechanical arm 16 moves to reaction cup in the useless cup groove 13-8 on cleaning station reaction cup, waste liquidPump 15 is taken waste liquid away by waste liquid pin group 13-7, and then mobile response cup traversing carriage, makes thisThrough hole on support moves on to the below of useless cup groove, this discarded reaction cup is fallen into discarded reaction cup logicalRoad 21, thus the outside of instrument drawn. For two-step method or the three-step approach of some reagent, clean knotShu Hou, micromachine arm 16 is not that reaction cup is sent into and measured darkroom, but reaction cup is sent againReturn reaction rotating disk 11, then carry out the process of application of sample, incubation, program is below identical with one-step method.
If what carry out is biochemical analysis, this reaction cup is turned to measuring position, now light source groupThrough hole on light transmission condenser, incubation dish 12 that the bulb of part sends, reaction rotating disk 11 sidesThrough hole 11-2 enter reaction cup, passed through spectrum by the light that reactant in reaction cup is selective after absorbingMeasure on the condenser, slit, beam splitting system, arrival photodetector of assembly, by photoelectricityThe optical signal of detector is measured, and the result of measuring is sent into computer 26 and process, justCan carry out Analysis of Biochemical. After measurement finishes, forward tested reaction cup to cup position,By micromachine arm 16, reaction cup is moved in the useless cup groove 13-8 on cleaning station reaction cup,Waste drains pump 15 is taken waste liquid away by waste liquid pin group 13-7, then mobile response cup traversing carriage,Make through hole on this support move on to the below of useless cup groove, this discarded reaction cup is fallen into discarded reactionCup passage 21, thus the outside of instrument drawn.
In order to reduce cross pollution, after each sampling and application of sample end cycle, sample needle all will be got back toScouring stage 5 rinses. By flushing pump 6, the inside and outside wall of pin is rinsed the liquid of flushingTaken away by drawing liquid pump 7.
In experimentation, can pass through reaction cup collating unit 22-1, slideway 22-2, funnel 22-3In reaction tray, constantly load reaction cup. Reaction cup collating unit 22-1 makes to be positioned at its hopperReaction cup falls in slideway 22-2 by definite orientation, falls into the cup of reaction cup via funnel 22-3In groove.
Above-mentioned all actions all have control circuit 25 to realize.
Finally it should be noted that: only also unrestricted the present invention in order to the present invention to be described of above embodimentDescribed technical scheme; Therefore, although this description with reference to the above embodiments to the present inventionHave been described in detail, still, those of ordinary skill in the art should be appreciated that still passableThis invention is modified or is equal to replacement; And all do not depart from the skill of the spirit and scope of the present inventionArt scheme and improvement thereof, it all should be encompassed in claim scope of the present invention.
Claims (5)
1. full-automatic biochemical and a luminescence immunoassay system, comprises the large machinery that can carry out XYZ motionArm, sample-adding pump, sample needle, scouring stage, flushing pump, drawing liquid pump, agent bin, sample storehouse, reaction rotating disk,Incubation dish, that vibration mixes assembly, micromachine arm, cleaning station assembly, scavenging pump, waste drains pump, measurement is darkChamber component, excite reagent pump, excite reagent needles, photon survey assembly, light source assembly, spectral measurement assembly,Discarded reaction cup passage, reaction cup automatic loading assembly, control circuit and computer, is characterized in that: largeMechanical arm can side-to-side movement in X-axis, and X-axis is mounted on the left and right support in system backplane, greatlyY-axis motion and Z axis motion are installed on mechanical arm, on Z axis motion, application of sample are installedPin, sample needle is connected with sample-adding pump by pipeline;
Cleaning station assembly is arranged on the outside of reaction rotating disk, is provided with measures darkroom in one end of cleaning station assemblyAssembly, is provided with photon survey assembly in one end of measuring darkroom assembly;
Described cleaning station assembly divides by reacting cup, reaction cup traversing carriage, reaction cup lifting support, magneticIn vitro, cleaning needle support, cleaning needle group, waste liquid pin group, useless cup groove composition, have several on reaction cupCup groove, is provided with magnetic chorista in part cup groove, and reaction cup lifting support and reaction cup traversing carriage are all installedOn reaction cup, lifting support is placed on again on traversing carriage, can make reaction cup by a motionLifting support lifting on reaction cup traversing carriage, another motion can drive traversing carriage, lifting to prop upFrame moves the position of a reaction cup groove;
Described measurement darkroom assembly by measuring darkroom pedestal, measure darkroom drawer guideway, measure darkroom drawer,Shadow shield, speculum composition, on drawer, have can placing response cup cup groove, deviate from photon at cup groove and surveyThe one side of amount assembly is placed with speculum, and above pedestal, be provided with and excite reagent needles, in the time that drawer is closed,Shadow shield is opened, and the cup groove on drawer is just in time positioned at before the detector of photon survey assembly, excites reagent needles alsoJust in time be positioned at a glass appropriate location for groove top;
Micromachine arm is arranged on reaction rotating disk, cleaning station assembly and measures between the assembly of darkroom its turning cylinderLine plays the distance of glass position on rotating disk and in cleaning station assembly, reacts on cup each glass of groove, useless glass to reactionThe distance of groove and with equate to the distance of measuring on the drawer of darkroom cup groove under open mode;
Spectral measurement position at incubation dish has through hole, in the both sides of through hole, light source assembly and spectrum is installedMeasure assembly.
2. a kind of full-automatic biochemical as claimed in claim 1 and luminescence immunoassay system, is characterized in that:Agent bin, incubation dish are installed on the base plate of system, are coaxially installed with reaction rotating disk above incubation dish,On reaction rotating disk, be evenly processed with multiple reaction cup grooves, bottom and the side of each glass of groove have through hole, turnDish can rotate around axis, and reaction cup slot part is in incubation dish.
3. a kind of full-automatic biochemical as claimed in claim 1 and luminescence immunoassay system, is characterized in that:Sample storehouse can be divided into basic storehouse and expansion storehouse, and coaxial installation the in basic storehouse is placed on reaction rotating disk inner side, expansion storehouseBe arranged on the base plate of reaction rotating disk opposite side, system can have basic storehouse and expansion storehouse simultaneously, also canOnly have basic storehouse or expansion storehouse.
4. a kind of full-automatic biochemical as claimed in claim 1 and luminescence immunoassay system, is characterized in that:Described vibration mixes assembly by push rod, oscillator, top rod driving installation composition, and its oscillator is connected with push rod,Vibration mixes assembly and is arranged on the below of incubation dish, on the incubation dish above push rod, has through hole, and push rod canTo push up the bottom of reaction cup through the through hole of through hole thus and reaction rotating disk bottom, oscillator drives by push rodReaction cup vibration.
5. a kind of full-automatic biochemical as claimed in claim 1 and luminescence immunoassay system, is characterized in that:Discarded reaction cup passage is arranged in the below of the useless cup of cleaning station assembly groove, and the bottom of useless cup groove is that reaction cup movesSupport, reaction cup traversing carriage has a through hole near the position of useless cup groove, mobile response cup traversing carriage,Can make this through hole move on to the below of useless cup groove.
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| CN107271701B (en) * | 2016-04-08 | 2019-06-11 | 北京德鲁伊医疗器材有限公司 | A kind of multi-purpose medical detector and detection system |
| CN106018784B (en) * | 2016-07-05 | 2019-02-22 | 深圳普门科技股份有限公司 | Small electrical chemical illumination immunity analysis instrument and its analysis method |
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