CN202075285U - Minitype rotating disc type full-automatic immunoluminescence analysis system capable of adding sample in whole disc - Google Patents
Minitype rotating disc type full-automatic immunoluminescence analysis system capable of adding sample in whole disc Download PDFInfo
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- CN202075285U CN202075285U CN201120094994XU CN201120094994U CN202075285U CN 202075285 U CN202075285 U CN 202075285U CN 201120094994X U CN201120094994X U CN 201120094994XU CN 201120094994 U CN201120094994 U CN 201120094994U CN 202075285 U CN202075285 U CN 202075285U
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Abstract
The utility model discloses a minitype rotating disc type full-automatic immunoluminescence analysis system capable of adding samples in the whole disc. The system comprises a left arm and a right arm, a sample adding pump, a sample adding needle, a reagent bin, a reagent box, a sample disc, a sample test tube, a reaction rotating disc, a reaction cup, an incubating groove, a minitype mechanical arm, a magnetic separation body, a cleaning station reaction cup base, a cleaning station reaction cup movable bracket, a cleaning station reaction cup lifting bracket, a cleaning needle bracket, a cleaning needle set, a biological waste liquid needle set, a cleaning pump, a biological waste liquid pump, a stimulation reagent pump, a measurement dark chamber, a lifting support plate, a photon measurement unit, a reflector, a chemical waste liquid needle, a chemical waste liquid pump, a discarded reaction cup channel and a computer, wherein the left arm and the right arm can perform X, Y and Z movements. Due to the adoption of the combination mode of XYZ movements, the minitype rotating disc and the minitype mechanical arm, the samples can be added in the whole disc, the flexibility of the system is greatly increased, the measurement speed and reliability can be improved, the volume of the system is greatly reduced, and the complex degree and the cost of the system are also reduced.
Description
Technical field
The utility model relates to the medical instruments and equipment field, the full-automatic immunoluminescence analytic system of the miniature rotating disc type of particularly a kind of overall application of sample.
Background technology
Along with science and technology development, chemiluminescence and biochemical luminous measurement had become very common in recent years, they often are used to determine in the sample certain not content of principal component, and in the past ten years, important effect has also been brought into play in luminous measurement in the research of gene expression and adjusting, chemiluminescence is the light that utilizes in the chemical reaction process to be excited, biochemical luminous is to utilize in chemical reaction process, to add the light that enzymaticization is sent.Chemical and biochemical luminous measuring technique is compared with other measuring technique, and following advantage is arranged: high sensitivity, wide dynamic range, the luminous measurement reagent that continues to bring out.Luminous measurement has high sensitivity, and it is higher by 10 than the sensitivity of spectral absorption measuring technique
5Doubly, exceed 1000 times at least than fluorescence measurement techniques.The luminous surveying instrument of an excellent in design can detect the luciferase of (ATP) the formula 0.1fg that is lower than 0.6pg.The luminous quantity of measurement in chemical reaction process is very useful because in the sample not the content of principal component can from this luminous quantity, calculate.Measuring equipment for luminescent system has semi-automatic and full-automatic two big classes at present.The semi-automatic measuring complicated operation needs operating personnel that higher experimental technique is arranged, and introduce personal error easily, and testing process is slower.And the luminous measuring system of full-automatic chemical mainly is divided into two major types, one is based on the measuring system of ELISA Plate technology, one is based on the tubular type measuring system of reaction cup, because the latter's sensitivity is higher, more can give full play to chemiluminescent advantage, therefore, adopt the mark and the isolation technics of the tubular type chemiluminescence measurement system incorporates nanometer magnetic bead of reaction cup to become the main flow that chemiluminescence is measured.The full-automatic chemical electrochemiluminescent immunoassay measuring system that more external well-known manufacturers are released all adopts this pattern.But all automatic measurement systemic-function in the market is powerful, complex structure (failure rate height), and the cost height only is fit to some large hospitals and uses, and is difficult to select present all automatic measurement system for a large amount of middle and small hospitals.For the ease of popularizing of luminous measuring method, be badly in need of the full-automatic chemical luminous diagnostic system of development based on the brand-new design of nanometer magnetic bead technology, can satisfy the requirement of large hospital to instrumental function and speed, can satisfy the low requirement of use cost of middle and small hospital again, this just requires the simple in structure of system, volume is little, and cost is low, failure rate is low, also is convenient to reviewer's operation and maintenance.
Tubular type full-automatic chemical luminescence immunoassay system in the market is by following mode operation substantially: sample and reagent are added reaction cup, make reaction cup be recycled to incubation respectively again, magnetic separates and purging system, makes reaction cup enter airtight darkroom at last and measures.In these systems, the employing that has the reaction cup of larger volume, the employing strip reaction cup that has.In order to make these reaction cup can arrive above-mentioned appointed area, need a cover complicated mechanical transport system.For some test event, must adopt two-step approach or three-step approach, just after cleaning for the first time, not reaction cup to be sent into measure the darkroom, but send reaction cup back to the application of sample zone again, carry out application of sample, incubation, magnetic separation and clean cycle again, until requirement by two-step approach or three-step approach, finished all operations, just reaction cup has been sent into the measurement darkroom at last and measured, thereby make general tubular type full-automatic chemical luminescence immunoassay system become very huge and complicated.
The utility model content
In order to overcome the deficiencies in the prior art, the pattern that the utility model provides a kind of XYZ of employing motion, miniature rotating disk and micromachine arm to combine, both can realize overall application of sample, increased the dirigibility of system greatly, improved measuring speed, and only need be with a micromachine arm, just can finish and reaction cup is sent to cleaning station carry out that magnetic separates and clean, sending to the measurement darkroom measures, or send rotating disk again back to and carry out again application of sample and incubation, so just greatly reduce the complexity and the cost of system, reduced the volume of system.
The utility model is made up of following 32 parts: the left arm and the right arm that can carry out the XYZ motion, sample-adding pump, sample needle, the reagent storehouse, kit, sample disk, sample tube, the minisize reaction rotating disk, reaction cup, the incubation groove, the micromachine arm, the magnetic chorista, cleaning station reaction cup, cleaning station reaction cup traversing carriage, cleaning station reaction cup lifting support, the cleaning needle support, the cleaning needle group, biological waste liquid pin group, scavenging pump, biological waste drains pump, excite reagent pump, measure the darkroom, measure the darkroom seal cover board, excite reagent needles, the chemical waste fluid pin, the chemical waste fluid pump, the lifting supporting plate, the photon survey unit, catoptron, discarded reaction cup passage, computing machine.During measurement, the transparent reaction cup is placed in the rotating disk, XYZ motion by left and right arms and sample-adding pump, sample needle are added to reagent in the kit and the sample in the sample tube in the reaction cup, reaction cup is carried out incubation in keeping the incubation groove of constant temperature, after the incubation time of certain reaction cup arrives official hour, the rotational response rotating disk moves to it on reaction cup of cleaning station by the micromachine arm to ad-hoc location.On the reaction cup of cleaning station several trough is arranged, can place several reaction cup.Cleaning station has the magnetic chorista to realize that magnetic separates, and cleaning station has and move the function that is put into the reaction cup on the cleaning station reaction cup, and mobile response cup one by one makes it successively by the magnetic separated region with clean the zone.Cleaning the zone, scavenging pump cleans reaction cup by the cleaning needle group of cleaning on the support, and waste liquid is taken away by biological waste drains pump and biological waste liquid pin group.After cleaning finishes, by the micromachine arm reaction cup is moved to again and measure the top, darkroom.Measuring top, darkroom has a seal cover board, plays the effect in plugging meter darkroom, on the cover board also is equipped with and excites reagent needles and waste liquid pin.In the inside of measuring the darkroom one elevating mechanism is arranged, a supporting plate is arranged on the elevating mechanism.When mechanical arm moved on to top, measurement darkroom with reaction cup, cover plate can be opened automatically, and the starting elevating mechanism makes mechanical arm reaction cup can be placed on the supporting plate, and elevating mechanism begins to descend then, the reaction cup on the supporting plate is dropped to measure in the darkroom.When dropping to a certain position, the seal cover board closure seals the darkroom fully, and elevating mechanism continues to descend, and reaction cup is reduced to the measuring position.Can program according to the rules excite reagent by exciting reagent pump to add by the reagent needles that excites on the seal cover board, the light of institute's output is measured by the photon survey unit, and the result of measurement sends into computing machine and handles.After measuring end, the measuring chamber elevating mechanism is sent measuring chamber with it, by the micromachine arm reaction cup is moved in the discarded reaction cup passage on the cleaning station reaction cup, and the waste liquid pin is taken waste liquid away, under the effect of a motion, the reaction cup that this is discarded falls into pail for used dressings then.If this test request adopts two-step approach or three-step approach, after then cleaning finished, the micromachine arm was not reaction cup to be sent into measure the darkroom, but sends reaction cup back to the application of sample zone again, carries out the process of application of sample, incubation again.
Left arm and right arm can side-to-side movements on X-axis, X-axis is mounted on the left and right sides support on the system backplane, Y-axis motion and Z shaft movement mechanism are installed on left arm and right arm, thereby realize the motion of the XYZ axle of left and right arms, on the Z axle, sample needle is installed, sample needle links to each other with sample-adding pump by plastic tube, thereby reagent in the kit and the sample in the sample tube can be added in the reaction cup, thereby, by XYZ motion of mechanism and sample needle, can carry out overall application of sample to the reaction cup of any position on the minisize reaction rotating disk.The reagent storehouse is installed on the base plate in system left side, kit is placed in the reagent storehouse, sample disk, reaction rotating disk, incubation groove as an integral installation on the back up pad on system right side, sample tube is put into sample disk, and reaction cup is put into the minisize reaction rotating disk that is in the incubation groove.The reaction cup of putting into miniature rotating disk adopts transparent material to make, and the reaction cup cross section is for trapezoidal, and along the direction of measuring optical axis, reaction cup is thinner, and perpendicular to the direction of measuring optical axis, the area of reaction cup is bigger.Cleaning station, the passage of measuring darkroom, waste drains pump and being connected to dustbin are installed on the base plate on system right side.Because reaction cup is put into cleaning station reaction cup from the rotating disk taking-up, after cleaning end reaction cup is delivered to the measurement darkroom, again reaction cup is thrown into the discarded reaction cup passage that is connected to dustbin after measuring end, these actions all are to be finished by same mechanical arm, therefore require mechanical arm on the rotating disk to get the position of reaction cup, the position of placing response cup on the cleaning station reaction cup, it is on the circumference of radius that the discarded reaction cup passage of measuring the inlet of darkroom placing response cup and being connected to dustbin all must be distributed in the mechanical arm, to guarantee the reliable access reaction cup of mechanical arm.
Owing to adopted above technical scheme, the utlity model has following beneficial effect: can satisfy the requirement of large hospital to instrumental function and speed, can satisfy the low requirement of use cost of middle and small hospital again, and because system architecture is simple, volume is little, reduce cost and failure rate, also be convenient to reviewer's operation and maintenance.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
In the drawings: the 1-left arm, the 2-right arm, the 3-sample-adding pump, the 4-sample needle, 5-reagent storehouse, the 6-kit, the 7-sample disk, the 8-sample tube, 9-reacts rotating disk, the 10-reaction cup, 11-incubation groove, 12-micromachine arm, 13-magnetic chorista, 14-cleaning station reaction cup, 15-cleaning station reaction cup traversing carriage, 16-cleaning station reaction cup lifting support, 17-cleaning needle support, 18-cleaning needle group, the biological waste liquid pin of 19-group, the 20-scavenging pump, the biological waste drains pump of 21-, 22-excites reagent pump, 23-measures the darkroom, 24-measures the darkroom cover plate, 25-excites reagent needles, 26-chemical waste fluid pin, 27-chemical waste fluid pump, 28-lifting supporting plate, 29-photon survey unit, the 30-catoptron, 31-discards the reaction cup passage, the 32-computing machine.
Fig. 2 is the structural representation of cleaning station of the present utility model.
Fig. 3 is the structural representation in measurement of the present utility model darkroom.
Fig. 4 is the structural representation of reaction cup of the present utility model.
Embodiment
As shown in Figure 1, the utility model provides the miniature rotating disc type of a kind of overall application of sample full-automatic immunoluminescence analytic system, comprise the left arm 1 and the right arm 2 that can carry out the XYZ motion, sample-adding pump 3, sample needle 4, reagent storehouse 5, kit 6, sample disk 7, sample tube 8, reaction rotating disk 9, reaction cup 10, incubation groove 11, micromachine arm 12, magnetic chorista 13, cleaning station reaction cup 14, cleaning station reaction cup traversing carriage 15, cleaning station reaction cup lifting support 16, cleaning needle support 17, cleaning needle group 18, biological waste liquid pin group 19, scavenging pump 20, biological waste drains pump 21, excite reagent pump 22, measure darkroom 23, measure darkroom cover plate 24, excite reagent needles 25, chemical waste fluid pin 26, chemical waste fluid pump 27, lifting supporting plate 28, photon survey unit 29, catoptron 30, discarded reaction cup passage 31 and computing machine 32.During measurement, transparent reaction cup 10 is placed in the rotating disk 9, XYZ motion by left and right arms and sample-adding pump 3 and sample needle 4 are added to the sample in reagent in the kit 6 and the sample tube 8 in the reaction cup 10, and reaction cup 10 is carried out incubation in keeping the incubation groove 11 of constant temperature.After the incubation time of certain reaction cup arrived official hour, the rotational response rotating disk moved to it on reaction cup 14 of cleaning station by micromachine arm 12 to ad-hoc location.Cleaning station reaction cup lifting support 16 and cleaning station reaction cup traversing carriage 15 all are installed on the cleaning station reaction cup 14.Lifting support 16 is placed on again on the traversing carriage 15, can make lifting support 16 lifting on traversing carriage 15 by a motion, lifting support 16 drives all reaction cup that are placed on the reaction cup 14 and rises together when rising, exceed the groove plane of reaction cup 14 up to the bottom of reaction cup, drive traversing carriage 15 by another motion this moment, all reaction cup move the position of a reaction cup on lifting support 16 and the lifting support, fall lifting support 16 again, all reaction cup above it are all dropped in the new groove, this moment, motion drove traversing carriage 15 and lifting support 16 is got back to reference position again, prepared circulation next time.Like this, cleaning station just has and moves the function be put into the reaction cup on the cleaning station reaction cup, mobile response cup one by one, and it is separated by magnetic successively and clean the zone.The magnetic of cleaning station separates by magnetic chorista 13 to be realized.Magnetic chorista 13 can be a natural magnet, also can be electromagnet.Cleaning the zone, scavenging pump cleans by 18 pairs of reaction cup of cleaning needle group of cleaning on the support 17, and waste liquid is taken away by biological waste drains pump 21 and biological waste liquid pin group 19.After cleaning finishes, reaction cup is moved to the top of measuring darkroom 23 by micromachine arm 12 again.Measuring top, darkroom has the darkroom of measurement seal cover board 24, plays the effect in plugging meter darkroom.On the cover board also be equipped with and excite reagent needles 25 and waste liquid pin 26.In the inside of measuring the darkroom one elevating mechanism is arranged, lifting supporting plate 28 is arranged on the elevating mechanism.When mechanical arm moves on to top, measurement darkroom with reaction cup, cover plate can be opened automatically, the starting elevating mechanism makes mechanical arm reaction cup can be placed on the supporting plate 28, and elevating mechanism begins to descend then, reaction cup on the supporting plate is dropped in the measurement darkroom, when dropping to a certain position, seal cover board 24 closures seal the darkroom fully, elevating mechanism continues to descend, and reaction cup is reduced to the measuring position.Can program according to the rules excite reagent by exciting reagent pump 22 to add by the reagent needles 25 that excites on the seal cover board, the light of institute's output is measured by photon survey unit 29, and the result of measurement sends into computing machine 32 and handles.In order better to collect luminous energy, be placed with the catoptron 30 of particular design on the opposite of photon survey unit 29, with the exciting light reflected back photon measuring unit of photon survey unit dorsad.After measuring end, the measuring chamber elevating mechanism is sent measuring chamber with it, by micromachine arm 12 reaction cup is moved in the discarded reaction cup passage 31 on the cleaning station reaction cup, chemical waste fluid pump 27 is taken waste liquid away by chemical waste fluid pin 26, under the effect of a motion, the reaction cup that this is discarded falls into pail for used dressings then.If this test request adopts two-step approach or three-step approach, after then cleaning end, micromachine arm 12 is not reaction cup to be sent into measure darkroom 23, but sends reaction cup back to reaction rotating disk 9 again, carry out the process of application of sample, incubation again, the program of back is then identical with single stage method.
Left arm 1 and right arm 2 can side-to-side movements on X-axis, X-axis is mounted on the left and right sides support on the system backplane, Y-axis motion and Z shaft movement mechanism are installed on left arm 1 and right arm 2, thereby realize the motion of the XYZ axle of left and right arms, sample needle 4 is installed on the Z axle, sample needle 4 links to each other with sample-adding pump 3 by plastic tube, thereby the sample in reagent in the kit 6 and the sample tube 8 can be added in the reaction cup 10.Reagent storehouse 5 is installed on the base plate in system left side, kit 6 is placed in the reagent storehouse, sample disk 7, reaction rotating disk 9, incubation groove 11 as an integral installation on the back up pad on system right side.Cleaning station reaction cup 14, cleaning station reaction cup traversing carriage 15, cleaning station reaction cup lifting support 16 as an integral installation on the back up pad on the right side of system, cleaning needle group 18, biological waste liquid pin group 19, chemical waste fluid pin 26 all are installed on the cleaning needle support 17, and cleaning needle support 17 is installed on the back up pad on system right side.Scavenging pump 20, biological waste drains pump 21, cleaning station, excite reagent pump 22, measure darkroom 23, right half part that chemical waste fluid pump 27, photon survey unit 29, discarded reaction cup passage 31 also all are installed in system.
Claims (11)
1. full-automatic immunoluminescence analytic system of the miniature rotating disc type of overall application of sample, comprise: the left arm and the right arm that can carry out the XYZ motion, sample-adding pump, sample needle, the reagent storehouse, kit, sample disk, sample tube, the reaction rotating disk, reaction cup, the incubation groove, the micromachine arm, the magnetic chorista, cleaning station reaction cup, cleaning station reaction cup traversing carriage, cleaning station reaction cup lifting support, the cleaning needle support, the cleaning needle group, biological waste liquid pin group, scavenging pump, biological waste drains pump, excite reagent pump, measure the darkroom, the lifting supporting plate, the photon survey unit, catoptron, the chemical waste fluid pin, the chemical waste fluid pump, discarded reaction cup passage and computing machine, it is characterized in that: left arm and right arm can side-to-side movements on X-axis, X-axis is mounted on the left and right sides support on the system backplane, and Y-axis motion and Z shaft movement mechanism are installed on left arm and right arm.
2. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1, it is characterized in that: on the Z axle sample needle is installed, sample needle links to each other with sample-adding pump by plastic tube.
3. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1, it is characterized in that: the reagent storehouse is installed on the base plate in system left side, kit is placed in the reagent storehouse, sample disk, reaction rotating disk, incubation groove as an integral installation on the back up pad on system right side, sample tube puts into sample disk, reaction cup is put into the minisize reaction rotating disk that is in the incubation groove.
4. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1, it is characterized in that: the reaction cup of putting into miniature rotating disk adopts transparent material to make, the reaction cup cross section is for trapezoidal, along the direction of measuring optical axis, reaction cup is thinner, perpendicular to the direction of measuring optical axis, the area of reaction cup is bigger.
5. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1 is characterized in that: by XYZ motion of mechanism and sample needle, can carry out overall application of sample to the reaction cup of any position on the minisize reaction rotating disk.
6. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1, it is characterized in that: after the incubation time of certain reaction cup in the rotating disk arrives official hour, the rotational response rotating disk moves to it on reaction cup of cleaning station by the micromachine arm to ad-hoc location.
7. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1 is characterized in that: on the reaction cup of cleaning station several trough is arranged, can place several reaction cup.
8. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1, it is characterized in that: cleaning station reaction cup lifting support and cleaning station reaction cup traversing carriage all are installed on the cleaning station reaction cup, can make lifting support lifting on traversing carriage by a motion, lifting support drives all reaction cup that are placed on the cleaning station reaction cup and rises together when rising, exceed the groove plane of reaction cup up to the bottom of reaction cup, drive traversing carriage by another motion this moment, the position that all reaction cup move a reaction cup on lifting support and the lifting support on the reaction cup, fall lifting support again, all reaction cup above it are all dropped in the new groove, this moment, motion drove traversing carriage and lifting support is got back to reference position again, prepared circulation next time.
9. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1, it is characterized in that: after cleaning finishes, the micromachine arm moves to reaction cup and measures the top, darkroom, measure top, darkroom one seal cover board is arranged, on seal cover board, be equipped with and excite reagent needles, in the inside of measuring the darkroom one elevating mechanism is arranged, one supporting plate is arranged on the elevating mechanism, when mechanical arm moves on to top, measurement darkroom with reaction cup, cover plate can be opened automatically, the starting elevating mechanism, make mechanical arm reaction cup can be placed on the supporting plate, elevating mechanism begins to descend then, and the reaction cup on the supporting plate is dropped to the measuring position, can program according to the rules excite reagent by exciting reagent pump to add by the reagent needles that excites on the seal cover board, the light of institute's output is measured by the photon survey unit.
10. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1, it is characterized in that: after reaction cup is measured and finished, the measuring chamber elevating mechanism is sent measuring chamber with it, by the micromachine arm reaction cup is moved in the discarded reaction cup passage on the cleaning station reaction cup, the waste liquid pin is taken waste liquid away, under the effect of a motion, the reaction cup that this is discarded falls into pail for used dressings then.
11. the full-automatic immunoluminescence analytic system of the miniature rotating disc type of a kind of overall application of sample as claimed in claim 1, it is characterized in that: be placed with the catoptron of particular design on the opposite of photon survey unit, the exciting light reflected back photon measuring unit of photon survey unit dorsad.
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| CN201120094994XU CN202075285U (en) | 2011-04-02 | 2011-04-02 | Minitype rotating disc type full-automatic immunoluminescence analysis system capable of adding sample in whole disc |
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| CN201120094994XU CN202075285U (en) | 2011-04-02 | 2011-04-02 | Minitype rotating disc type full-automatic immunoluminescence analysis system capable of adding sample in whole disc |
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