WO2018126773A1 - Automatic analyzer and sample analysis method - Google Patents
Automatic analyzer and sample analysis method Download PDFInfo
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- WO2018126773A1 WO2018126773A1 PCT/CN2017/108327 CN2017108327W WO2018126773A1 WO 2018126773 A1 WO2018126773 A1 WO 2018126773A1 CN 2017108327 W CN2017108327 W CN 2017108327W WO 2018126773 A1 WO2018126773 A1 WO 2018126773A1
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- reaction
- reaction vessel
- unit
- transfer
- incubation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/0092—Scheduling
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- the invention relates to the field of in vitro diagnostic equipment, in particular to an automatic analysis device and a sample analysis method.
- the present invention is described in the context of a fully automated immunoassay instrument in In-Vitro Diagnostics (IVD), in particular, a luminescent immunoassay analyzer, which should be understood by those skilled in the art.
- IVD In-Vitro Diagnostics
- the protocols and methods of the present invention are also applicable to other clinical test automation devices, such as fluorescent immuno devices, electrochemical immunization, and the like.
- the automatic immunoassay analyzer usually consists of a sampling unit, a reaction unit, a supply and waste waste unit, a system control unit, and the like.
- Luminescence immunity has become the mainstream technology of automatic immunity due to its advantages of quantitative detection, high sensitivity, good specificity, wide linear range and high degree of automation.
- the fully automatic luminescence immunoassay differs according to the labeling method and the luminescence system, and includes enzymatic chemiluminescence, direct chemiluminescence, and electrochemiluminescence.
- the luminescence immunoassay can be generally divided into a one-step method, a one-step one-step method, a two-step method, etc. according to the test principle and mode.
- the main test steps generally include adding a sample and a reagent, mixing the reactants, and incubating. Cleaning separation (Bound-Free, referred to as B/F), adding signal reagents, measuring, etc.
- B/F Cleaning separation
- the present invention distinguishes between reagents and signal reagents, incubation and signal incubation for ease of presentation.
- the reagents and analysis items have a “one-to-one correspondence” relationship, that is, the specific reagents corresponding to different analysis items generally differ in formula, reagent amount, and component quantity.
- the reagent typically includes multiple components, such as the usual 2-5 components, including reagent components such as magnetic particle reagents, enzyme labeling reagents, diluents, and the like.
- reagent components such as magnetic particle reagents, enzyme labeling reagents, diluents, and the like.
- multiple reagent components of an analysis item can be filled in one time or in multiple steps. When the step is added, the first reagent, the second reagent, and the third reagent are defined according to the order of filling. Wait.
- the signal reagent is used to measure the generation of the signal, usually a kind of general-purpose reagent, and the analysis item is "one-to-many" correspondence, that is, different analysis items share the signal reagent.
- the incubation of the present invention specifically refers to the process of antigen-antibody binding reaction or biotin-avidin binding reaction of the reactants in the constant temperature environment of the reaction unit before the start of washing and separation, specifically, one-step incubation, To enter an incubation prior to wash separation, delay the incubation by one step in a single step, including the first incubation before the second reagent is added and the second incubation before the wash separation, and the two-step incubation twice, including the first The first incubation before the separation is separated and the second incubation before the second cleaning separation.
- the signal incubation refers to a process in which the reaction vessel after the separation and separation is added to the signal reagent and reacted in a constant temperature environment for a period of time to enhance the signal.
- the reaction vessel after the separation and separation is added to the signal reagent and reacted in a constant temperature environment for a period of time to enhance the signal.
- tests that require signal incubation are typically enzymatic chemiluminescence immunoassays.
- the test steps corresponding to different test modes are detailed as follows:
- One-step method Refer to Figure 1, add sample (S) and reagent (R), mix (some test methods can also do not need to mix, the same below, no longer repeat), incubate (generally 5-60 Minutes), after the incubation is completed, wash and separate, add signal reagent, signal incubation (usually 1-6 minutes), and finally measure. It should be pointed out that some luminescent systems do not require signal incubation due to the specific composition of the signal reagents. During the filling of the signal reagents or after the filling of the signal reagents It can be measured directly afterwards.
- the signal reagent may be one or more.
- the signal reagent includes a first signal reagent and a second signal reagent.
- One-step delay method the difference from the one-step method is that the reagent is added twice, and requires two incubations. After the first reagent is mixed, the first incubation is performed, and after the first incubation, the second reagent is added. Mix well. One more incubation, reagent addition, and mixing action than the one-step method, and the rest of the procedure is the same as the one-step method.
- Two-step method The difference from the one-step method is that there is one more cleaning and separation step, and the other steps are the same.
- the first prior art solution separates the incubation, wash separation, and measurement into separate layouts, each of which is accomplished by three rotating disks, and the reaction vessel is transferred between the different units by a mechanical gripper.
- the technical scheme has many components and units, the incubation transfer position, the cleaning separation transfer position and the measurement transfer position are dispersed on different discs, and the distance is long, and the reaction container needs to be transferred between the transfer positions, resulting in large volume and high cost. There are many problems such as many moving paths and complicated control processes.
- a second prior art solution arranges the incubation and measurement together to form an incubation measurement unit, which is performed by another separate unit, although the technical solution reduces one measurement disc compared to the first prior scheme. To a certain extent, it is advantageous to control the size and cost of the whole machine, but there are also the same problems as the first technical solution.
- the incubation measurement unit In order to achieve flexible incubation time, the incubation measurement unit is complicated to control, and the incubation and measurement are also controlled by each other. There are not only shortcomings such as high-speed automated testing, but also flexible signal incubation.
- a third prior art solution achieves incubation, wash separation and measurement on a single-turn disc or a trajectory track.
- the disc In order to support longer incubation times, the disc needs to be set in addition to cleaning separation and measurement positions. A lot of incubation positions, so in order to achieve high-speed testing, the size of the disc or the trajectory track needs to be designed to be large, difficult to manufacture, and costly.
- at least two A loading mechanism and at least two cleaning separation mechanisms increase material, processing, production costs and overall machine size.
- this technical solution also limits the incubation time, resulting in problems such as a fixed incubation time and an excessively long time.
- this technical solution is not only difficult to achieve the darkroom environment required for measurement, it requires an additional shutter mechanism, and flexible signal incubation is not possible.
- the present invention provides an automatic analysis device and a sample analysis method with low manufacturing cost, simple and compact structure, flexible and efficient test procedure or method.
- an automatic analysis device comprising: a filling unit, filling a sample and/or a reagent into a reaction vessel, a reaction unit, incubating and washing the reactants in the separation reaction vessel, measuring the device, measuring the reaction a reaction signal in the vessel, a transfer unit, transferring the reaction vessel between different positions, the reaction unit comprising a rotating device, the rotating device is provided with a plurality of reaction vessel positions for carrying and fixing the reaction vessel, the reaction At least one incubation transfer site and at least one wash separation transfer site are disposed on the unit, the at least one incubation transfer site of the reaction unit and the at least one wash separation transfer site being within a horizontal range of motion of the transfer unit.
- a sample analysis method comprising: a filling step of filling a sample and/or a reagent in a reaction vessel, and an incubating step of performing a reaction vessel entering the reaction unit through at least one incubation transfer position Incubating, washing and separating the step, washing and separating the reaction vessel entering the reaction unit through at least one washing and separating transfer position, removing unbound components in the reactant, adding a signal reagent step, adding a signal reagent to the reaction container, and measuring In the step, the reaction signal in the reaction vessel is measured by a measuring device.
- the invention realizes the incubation and washing separation of the reactants in the reaction vessel centering on the reaction unit, and at least one incubation transfer position and at least one cleaning separation transfer position are arranged on the reaction unit, and the transfer unit can be transferred between the incubation transfer position and the cleaning separation position.
- the reaction vessel can not only realize flexible incubation, but also solve the problem that the two-step test must be implemented by using multiple cleaning and separating mechanisms in the prior art, and fully realize efficient cleaning separation.
- the measuring device can be flexibly arranged or arranged according to the needs of the whole machine layout or structure realization, for example, can be directly installed on the reaction unit, set in a separate position or mounted on an independent measuring disk, etc., and solves the measurement in the prior art.
- the device layout is limited, and the measurement environment is easy to interfere.
- the invention improves the working efficiency of the analysis device and reduces the difficulty of realizing the realization of the automation function, and is well solved
- the technical problems of large size, slow detection speed, high cost and poor performance of the automatic instrument not only save the laboratory space, improve the test efficiency, but also help reduce the expenses, reduce the burden on the testee, and ultimately save a lot of Natural and social resources.
- Figure 1 is a schematic diagram of a one-step reaction mode
- Figure 2 is a schematic diagram of a one-step reaction mode (another signal measurement mode);
- Figure 3 is a schematic diagram of a one-step and two-step reaction mode
- Figure 4 is a schematic view showing a first embodiment of the automatic analyzer of the present invention.
- Figure 5 is a one-step test flow chart
- Figure 6 is a flow chart of a one-step delay test
- Figure 7 is a two-step test flow chart
- Figure 8 is a schematic view showing a second embodiment of the automatic analyzing device of the present invention.
- Figure 9 is a schematic view showing a third embodiment of the automatic analyzer of the present invention.
- Figure 10 is a schematic view showing a fourth embodiment of the automatic analyzer of the present invention.
- Figure 11 is a schematic view showing a fifth embodiment of the automatic analyzer of the present invention.
- An automatic analysis device of the present invention comprises: a filling unit, filling a sample and/or a reagent into a reaction vessel, a reaction unit, incubating and washing the reactants in the separation reaction vessel, measuring the device, and measuring the reaction signal in the reaction vessel a transfer unit that transfers the reaction vessel between different positions, the reaction unit comprising a rotating device, wherein the rotating device is provided with a plurality of reaction vessel positions for carrying and fixing the reaction vessel, and at least one of the reaction units is disposed Incubating the transfer site and at least one wash separation transfer site, at least one incubation transfer site and at least one wash separation transfer site of the reaction unit are within a range of horizontal motion of the transfer unit.
- the reaction vessel provides a reaction site for the reaction of the sample and the reagent, and may be a reaction tube of various shapes and configurations, a reaction cup, a reaction cup of a plurality of chambers, a reaction chip, etc., and is generally used at one time.
- the material of the reaction vessel is usually a plastic such as polystyrene.
- the reaction container may be coated with an antigen or an antibody in advance on the inner wall, or may be pre-stored with a magnetic bead or a plastic ball. The storage and supply of the reaction vessel is completed by the reaction vessel supply unit.
- the reaction vessel supply unit is preferably pre-arranged, and the reaction vessel is pre-arranged in the reaction vessel tray, the box or the reaction vessel rack, and the channel, and the reaction vessel supply unit can be used for the whole tray, the whole box of reaction vessels or a row at a time.
- a series of reaction vessels are delivered to the target location.
- the reaction vessel supply unit may be a silo type, and the reaction vessel may be poured into a silo of the reaction vessel supply unit in a scattered manner, and then the reaction vessel supply unit automatically sorts the reaction vessels one by one, and supplies the reaction vessel to the transfer unit. .
- the transfer of the reaction vessel between different locations in the apparatus of the invention can be accomplished by the transfer unit.
- the transfer unit can be any suitable mechanism for transferring or moving the reaction vessel.
- the preferred transfer unit of the present invention primarily comprises a drive mechanism, a horizontal motion robot arm, a pick and place mechanism, and the like.
- the pick and place mechanism is usually a mechanical finger, which can hold the reaction container.
- the horizontal motion mechanical arm can be driven along the X direction, the Y direction, the X direction and the Y direction, the radial direction, the circumferential direction, the radial direction and the circumferential direction by the driving mechanism.
- the transfer unit can also move up and down, placing the reaction vessels in different positions or taking them out from different locations.
- one or more transfer units can be set.
- the filling unit completes the filling of the sample and the reagent.
- the filling unit is generally composed of a steel needle or a disposable nozzle (Tip), a motion driving mechanism, a syringe or a liquid injection pump, a valve, a fluid line, and a cleaning tank (or a cleaning pool when a Tip is used).
- the filling unit can move horizontally in addition to the up and down movement, and the horizontal movement usually has several movement forms such as rotation, X direction and Y direction, and a combination thereof.
- the filling unit can be one, adding both the sample and the reagent, which makes the structure of the whole machine more compact and lower cost.
- the filling unit may further comprise one or several sample filling units, one or several reagent filling units, the sample filling unit only filling the sample or filling the sample and a part of the reagent, and the reagent filling unit is filling the reagent.
- the invention may also include a filling station.
- the filling station is located within the range of motion of the transfer unit and the filling unit or can be moved by horizontal motion to the range of motion of the transfer unit and the filling unit.
- the filling station receives and carries the reaction vessel transferred from the transfer unit, and accepts the filling unit to fill the reaction container with the sample and the reagent.
- a reaction vessel is placed on the filling station for placing a reaction vessel to which the sample and reagents need to be filled.
- the present invention preferably integrates the mixing mechanism at the filling station, and ultrasonically mixes and biases the reaction container after each filling. Rotate or shake to mix.
- a mixing mechanism such as an ultrasonic generator, into the filling unit, and mix the ultrasonic waves generated by the filling unit at the same time as the filling of the sample and the reagent or after the filling operation is completed.
- the filling station may not integrate the mixing mechanism, and the mixing may be performed by the suction and discharge action or the impact force of the filling unit.
- the reaction unit carries and fixes the reaction vessel.
- the reaction unit mainly includes a heat preservation device, a rotation device, and a cleaning separation device.
- the periphery of the heat preservation device usually has insulation materials such as heat insulating cotton, and usually wraps or surrounds the bottom, the periphery and the upper portion of the rotating device, and the heating device and the sensor may be disposed on the inner side of the side or the bottom, and the upper portion is generally a structure such as a cover plate to provide a constant temperature for the reaction unit. Incubate the environment and prevent or reduce the loss of heat in the reaction unit. Of course, for higher heat transfer efficiency, the heating device can also be mounted on the rotating device.
- the holding device can also support and secure the magnetic field generating device of the cleaning separation device to provide a magnetic field environment for cleaning separation.
- the heat insulating device can not only provide a mounting position for the photometric device, but also a darkroom environment required for the photometric device.
- the rotating device comprises a driving device, a transmission mechanism and an associated control circuit, etc., and controls and drives the rotating device to rotate at a fixed angle every fixed time (such as a cycle or a cycle), and forwards the reaction vessel to a certain position. (such as advancing a reaction container bit).
- the rotating device is provided with a plurality of independent holes, slots, brackets, bases or other structures suitable for carrying the reaction vessel, defined as the reaction vessel position.
- the reaction vessel can also hold the reaction vessel.
- fixed herein is meant that the reaction vessel does not move or slide within the reaction vessel position, but can move integrally with the reaction vessel site. In this way, the reaction vessel and the reaction vessel can be tightly packed, and the gap is smaller, which not only facilitates heat transfer incubation and precise positioning of the reaction vessel, but also makes the structure of the rotating device more compact, accommodates more reaction vessel positions, and has manufacturing costs.
- the reaction unit In addition to carrying and immobilizing, the reaction unit also incubates the reactants in the reaction vessel. For tests that require signal incubation, the reaction unit of the present invention can also implement a signal incubation function.
- the transfer position is defined as the fixed position of the reaction vessel in and out of the reaction unit on the reaction unit within the horizontal motion range of the transfer unit, which does not rotate with the rotation of the reaction vessel position.
- the reaction vessel position at different positions can be transferred and positioned to the transfer position under the rotation of the rotating device, and the reaction vessel can be received or withdrawn from the reaction vessel to complete the subsequent corresponding functions, such as entering the reaction unit for incubation or transferring to other reactions.
- the container position is cleaned and separated.
- the transfer position can be divided into an incubation transfer position and a wash separation transfer position.
- the present invention defines a transfer site through which the reaction vessel to be incubated into the reaction unit, after a certain period of incubation or at the end of the incubation, and the transfer site through which the reaction unit is transferred is defined as the incubation transfer position; after a certain period of incubation or after the end of the incubation, the separation needs to be cleaned.
- the transfer site through which the reaction vessel enters the reaction unit or/and the reaction vessel that completes the purge separation is transferred to the reaction unit is defined as the wash separation transfer site.
- the incubation unit of the present invention has at least one incubation transfer position, and the specific incubation transfer position can be inaccessible, only in or out, and into the reaction container as needed, so that flexible incubation time and various machine layouts can be realized.
- the reaction vessel that enters and exits the reaction unit by incubating the transfer site includes a reaction vessel that needs to be incubated once, incubated two or more times, so that it may be incubated once or twice. And more times the reaction vessel is concentrated in the first step, thereby reducing the size of the reaction unit and increasing the space utilization rate of the reaction unit.
- the cleaning separation transfer position can be set one by one, and the reaction container can be both retracted and further, so that the cleaning separation device can be made more compact, and at least one can be set, so that the cleaning separation device can be arranged more flexibly.
- the setting of the transfer bit on the reaction unit solves the present
- the problem that the transfer sites are dispersed on a plurality of different units and the reaction vessels that need to be incubated are dispersed can not only make the transfer unit have fewer moving paths, shorter distances, but also make full use of the space of the reaction unit, thereby making the whole machine Control is simpler and smaller.
- the reaction unit can also perform cleaning separation to remove unbound components of the reactants.
- the cleaning and separating device of the reaction unit of the present invention comprises a magnetic field generating device and a flushing mechanism.
- the magnetic field generating device provides a magnetic field environment for adsorbing paramagnetic particles in the reaction vessel to the inner wall of the reaction vessel. Due to factors such as response time, moving distance and resistance in the magnetic field, it takes a certain time for the paramagnetic particles to adsorb to the inner wall of the reaction vessel, usually ranging from several seconds to several tens of seconds, so that each time the waste liquid is taken (including unbound Before the component), the reaction vessel needs to pass through the magnetic field for a period of time.
- the magnetic field generating device can be directly mounted or fixed on the heat insulating device of the reaction unit, which not only saves an additional fixing mechanism, reduces the cost, but also brings the magnet generating device closer to the reaction container. Thereby reducing the adsorption time of the paramagnetic particles and improving the cleaning separation efficiency.
- the rinsing mechanism includes a liquid absorbing and injecting device that sucks unbound components in the reaction vessel and injects a washing buffer into the reaction after suction.
- the liquid absorption device comprises a liquid absorption part suitable for pumping liquid, such as a liquid suction needle, a liquid suction tube or a liquid suction nozzle, and the liquid absorption part is arranged above the reaction unit, and the reaction container can be driven into and out of the reaction container through the driving mechanism.
- the unbound components in the reaction vessel are aspirated.
- the liquid injection device includes a liquid injection portion suitable for discharging liquid, such as a liquid injection needle, a tube, a mouth, and the like, and the liquid injection portion is also disposed above the reaction container position of the reaction unit, and the cleaning buffer is injected into the reaction container after the suction.
- Each flush includes one aspirate and one injection buffer and process, usually three or four times, ie three or four rinses, although the number of flushes can be varied.
- the mixer In order to make the cleaning more thorough and less residue, it is also possible to set the mixer to mix the reaction vessel in the filling position or to use the impact force when injecting the liquid, and to make the paramagnetic particles heavy after the injection buffer or the cleaning buffer. Suspended and uniformly dispersed in the wash buffer.
- the reaction unit rotating device transfers the reaction vessel to the washing and separating device, the washing and separating device starts cleaning and separating the reaction vessel.
- the cleaning and separating device may further couple the signal reagent filling mechanism, and after the cleaning and separation of the reaction container, all or part of the signal reagent is added thereto, for example, all the first and second signal reagents are added. Wait for or only add the first signal reagent, etc., and the remaining signal reagents can be added during the measurement. This makes full use of the function of the cleaning and separating mechanism, reducing the size of the mechanism and saving costs.
- the cleaning and separating device is arranged around the rotating unit of the reaction unit or above the rotating device, and the reaction container on the rotating unit of the reaction unit can be directly cleaned and separated, thereby avoiding the installation of independent cleaning and separating rotating device, such as independent cleaning. Separating the disc or cleaning the separation rail, etc., not only streamlining the components and the whole mechanism, making the whole mechanism more compact and lower in cost, but also avoiding the transfer of the reaction vessel between the independent cleaning and separating device and the reaction unit, so that the whole machine Control processes are simpler and more efficient, increasing processing efficiency and reliability.
- the measuring device measures the signal in the reaction vessel.
- the signal is an electrical signal, a fluorescent signal or a weak chemiluminescence signal generated after the signal reagent is added to the reaction vessel.
- the measuring device includes a weak photodetector photomultiplier tube (PMT) or other sensitive photoelectric sensing device that converts the measured optical signal into an electrical signal for transmission to the control center.
- the measuring device may further include optical devices such as optical signal collection and calibration. Taking the weak chemiluminescence signal as an example, in order to avoid interference of ambient light, the measuring device of the present invention has three implementations for measuring the signal in the reaction container.
- the measuring device is installed in the reaction unit, and the reaction signal in the reaction vessel at the reaction vessel reaction vessel position is measured. This makes full use of the reaction vessel position on the reaction unit, making the machine more compact and less costly.
- the second embodiment includes measuring the darkroom and the measuring position, and the measuring device is mounted on the measuring darkroom to measure the signal in the reaction vessel at the measuring position. The measurement position is within the horizontal range of motion of the transfer unit or can be moved horizontally to the horizontal range of motion of the transfer unit.
- the third embodiment mainly includes a measuring disk, a measuring dark room, a measuring device, and the like. The measuring disk comprises at least one reaction vessel position centered on the center of rotation of the measuring disk for carrying a reaction vessel to be measured.
- the reaction vessel position on the measurement pan also enables signal incubation.
- the reaction vessel on any of the reaction vessel positions can be rotated to the measuring device for measurement, thereby achieving flexible signal incubation and improving test flexibility and efficiency.
- the measuring darkroom of the measuring unit is wrapped or enclosed around the measuring disc to provide a closed darkroom environment for the measuring unit.
- the heating device and the sensor may be disposed at the side or the bottom of the measurement darkroom to provide a constant temperature incubation environment for the measurement unit and prevent or reduce the loss of heat of the reaction unit.
- the heating device can also be mounted on the measuring plate.
- the measuring device can be connected or mounted to the measuring darkroom in a general manner, such as directly mounted on the measuring darkroom or mounted to the measuring darkroom via a fiber optic connection, so that the signal in the reaction vessel on the measuring disc reaction vessel can be directly measured. Can make processing efficiency and reliability higher.
- the measuring device of the invention can be flexibly arranged according to the design requirements, not only easy to realize the darkroom environment, but also realize flexible signal incubation, and solves the defects of the complicated structure of the prior art darkroom and the difficulty of the layout of the measuring device.
- the automatic analyzer of the present invention may further be provided with a unit such as a sample transport unit, a reagent storage unit, and the like.
- the sample transport unit is used to place the sample tube to be inspected and deliver the target sample tube to the sample site.
- the sample transport unit has three main modes: orbital injection, sample tray injection and fixed area injection.
- the sample tubes are usually placed on the sample holder. Each sample holder is usually placed with 5 or 10 sample tubes, and the sample holder is placed in the transmission. A fixed area on the track, on the sample tray, or on the analysis device.
- the reagent storage unit refrigerates the reagent and transfers the target reagent to the aspirating reagent position.
- the reagent storage unit usually adopts two ways of reagent tray and fixed reagent storage area.
- the reagent tray In order to ensure the stability of the reagent, the reagent tray generally has a cooling function, such as 4 - 10 °C.
- a plurality of reagent container positions are generally set on the reagent tray for placing the reagent container.
- Each reagent container is provided with a plurality of independent chambers for storing different reagent components, such as magnetic particle reagents, enzyme labeling reagents, diluents and the like.
- the automatic analysis device 100 mainly includes a sample delivery unit 30, a reagent storage unit 40, a filling unit 20, a filling station 90, a reaction container supply unit 70, a transfer unit 50, a reaction unit 10, a measuring device 86, and the like. The functions and functions of each part are described below.
- the sample delivery unit 30 is used to place the sample tube 31 to be inspected and deliver the target sample tube to the sample site.
- the sample transport unit 30 is a sample tray on which a curved sample holder (not shown) is placed, and each of the curved sample holders is placed with 10 sample tubes 31.
- the sample tray can be driven by the driving mechanism to transfer the target sample to the suction sample position under the control of the control center, and the suction sample position is located at the intersection of the horizontal movement range of the filling unit 20 and the center circle of the sample tube.
- the reagent storage unit 40 refrigerates the reagent container 41 and transfers the target reagent to the aspirating reagent position.
- the reagent storage unit 40 is a reagent tray, and 25 reagent positions are provided, and 25 reagent containers 41 (or kits and reagent bottles are conveniently used for the description, hereinafter referred to as reagent bottles).
- each of the reagent bottles 41 is provided with four chambers 41a, 41b, 41c, and 41d, and can be used for storing reagent components such as magnetic particle reagents, enzyme labeling reagents, and diluents.
- the reagent tray can be driven by the driving mechanism to transfer the target reagent bottle to the suction reagent position under the control of the control center.
- the suction reagent position is located at the intersection of the horizontal movement range of the filling unit and the center circle of the reagent chamber.
- the corresponding 4 Corresponding to the reagent components, there are 4 aspirating reagent sites (not shown).
- the filling unit 20 completes the filling of the sample and the reagent.
- the horizontal movement range of the filling unit intersects the sample position on the sample tray 30, the reagent position on the reagent disk 40, and the reaction container position on the measuring disk, respectively, and the intersection point is the suction sample position, the suction reagent position, and the filling position.
- the filling unit is a single sample loading mechanism, which can perform up and down and horizontal rotation movements, and both the sample and the reagent are added, so that the structure of the whole machine is more compact and the cost is lower.
- a mixing mechanism such as an ultrasonic generator may be integrated on the filling unit 20 to ultrasonically mix the reaction container after each filling.
- the filling station 90 is located under the horizontal movement track of the transfer unit 50 and the filling unit 20, and receives and transfers the reaction container transferred from the transfer unit 50, and accepts the filling unit 20 to fill the reaction container with the sample and the reagent.
- a reaction vessel is placed on the filling station for placing a reaction vessel to which the sample and reagents need to be added.
- the mixing mechanism is integrated in the filling station, and the reaction container after each filling is ultrasonically mixed or eccentrically oscillated and mixed, preferably eccentrically oscillating and mixing, so that the technology is less difficult to implement and the structure is more compact.
- the reaction vessel supply unit 70 stores and supplies a reaction vessel.
- the reaction container supply unit adopts a pre-arranged type.
- the reaction vessel supply unit 70 includes two reaction vessel trays on which a number of reaction vessel positions are disposed to store unused reaction vessels.
- Reaction vessel supply Unit 70 is within the horizontal range of motion of transfer unit 50 such that transfer unit 50 can traverse unused reaction vessels at each reaction vessel location on the tray to provide an unused reaction vessel for the newly initiated test.
- the transfer unit 50 transfers the reaction vessels between different positions of the automated analysis device 100.
- the transfer unit 50 is set to one, and the three-dimensional movement can be performed, which makes the whole machine more compact and lower in cost.
- the transfer unit 50 includes an X-direction moving robot arm 50b, a Y-direction guide rail 50a, a Y-direction moving robot arm 50c, and a vertical motion mechanism and a mechanical finger (not shown).
- the transfer unit 50 can simultaneously move the mechanical finger horizontally along the X direction and the Y direction, and the horizontal movement range covers the range within the boundary rectangle 56, and the 9 incubation transfer positions of the reaction container on the reaction container supply unit 70 and the reaction unit 10 can be 12a 1-3 , 12b 1-3 , 12c 1-3 ), 2 cleaning separation transfer sites (12d 1 and 12d 2 ) on the reaction unit 10, and transfer between the lost reaction vessel sites 60.
- the transfer unit since the range of motion of the transfer unit 50 covers a plurality of incubation transfer sites on the reaction unit 10, the transfer unit can be placed in the reaction vessel through different incubation transfer positions or transferred from the different incubation transfer sites to achieve a flexible incubation time. .
- the reaction unit 10 carries and fixes the reaction vessel, incubates and cleans the reactants in the separation reaction vessel.
- the heat retaining device of the reaction unit 10 is a pot body 12 and an upper cover (not shown), and the rotating device is a reaction disk 11 and a cleaning and separating device 16 .
- a heater and a sensor are arranged on the side or the bottom side of the pot body 12, surrounding the bottom and the periphery of the reaction disk 11, providing a constant temperature incubation environment for the reaction unit 10 to prevent or reduce the loss of heat of the reaction unit 10.
- the pot 12 also supports and secures the magnetic field generating means of the cleaning separation device 16 to provide a magnetic field environment for cleaning separation.
- the magnet generating device of the cleaning and separating device 16 is a permanent magnet device, which can provide a stronger and more stable magnetic field environment.
- the rinsing mechanism of the cleaning separation device 16 includes a liquid absorbing device and a liquid injection device, and a mixing mechanism.
- the cleaning and separating device 16 can also be coupled with a signal reagent filling mechanism, and after the cleaning container is separated from the reaction vessel at the reaction vessel position of the reaction unit 10, all or part of the signal reagent is filled therein.
- the measuring device 86 in this embodiment is directly mounted on the side of the pot body 12, and of course can also be mounted on the upper cover of the heat retaining device, and can directly measure the reaction signal in the reaction vessel on the reaction vessel position of the reaction disk 11. . This makes full use of the reaction vessel position on the reaction unit, making the machine more compact and less costly.
- the reaction disk 11 of the reaction unit 10 is rotatable about a central axis, and a four-turn reaction vessel position centered on the center of rotation is disposed thereon.
- the number of turns can be changed, but at least 2 turns, for example, 2 cycles, 3 turns, 5 turns or more, etc., a plurality of reaction vessel positions are arranged per turn, and the number of reaction vessel positions per turn may be the same or different.
- 30 reaction vessel positions are set per turn.
- Each reaction vessel is located in a suitable size tank on the reaction tray, and can accommodate a reaction vessel for carrying and fixing the reaction vessel. After the reaction vessel is placed in the corresponding reaction vessel position, no movement or sliding occurs in the reaction vessel position.
- the reaction vessel position on the three turns 11a, 11b, 11c in the reaction tray achieves an incubation function to accommodate the reaction vessel being incubated.
- the reaction vessel position on the outer ring 11d mainly accommodates the separation of the reaction vessel after the end of the incubation or after a certain period of incubation, mainly to achieve the cleaning separation and measurement functions.
- an opening is provided on the upper cover of the thermal insulation device, and the position of the opening is the transfer position, and a total of 9 incubation transfers are set.
- Positions 13a 1-3 , 13b 1-3 , 13c 1-3 and 2 wash separation transfer sites 13d 1 and 13d 2 , wherein the incubation sites 13a 1-3 , 13b 1-3 , 13c 1-3 correspond to the reactions
- Three turns 11a, 11b, 11c in the tray are respectively supplied to the reaction vessel in the reaction vessel 11a, 11b, 11c; the cleaning separation transfer sites 12d 1 and 12d 2 correspond to the reaction disk outer ring 11d, and the reaction vessel enters and exits 11d.
- the reaction plate is rotated at a fixed angle every fixed time and can be rotated counterclockwise or clockwise, for example by 12 degrees every 30 seconds, advancing a reaction vessel position.
- the reaction vessel at the reaction vessel can be transferred to the incubation transfer site or to the wash separation site.
- the transfer unit can shift the reaction vessel from the plurality of incubation transfer positions and the cleaning separation into and out of the reaction tray during the intermittent time after each rotation of the reaction tray.
- the reaction vessel After the reaction vessel enters the reaction disk by incubating the transfer site, it begins to incubate in the reaction vessel at 11a, 11b or 11c, and is transferred from the incubation transfer site after the incubation or incubation for a certain period of time.
- the reaction vessel that enters and exits the reaction disk by incubating the transfer site includes a reaction vessel that is incubated once, incubated twice or more, so that the space of the reaction disk can be fully utilized.
- reaction vessel can be incubated on the inner three rings 11a, 11b, 11c, and then transferred to the outer ring 11d for washing and separation, or after a certain period of incubation in the inner three rings, for example, most of the time is completed. Incubate, transfer to the outer ring 11d, and then complete the incubation for the remainder of the time while the reaction disk is transferred to the magnetic separation device.
- the former implementation it is possible to support the completion of the incubation of the reaction vessel without the need for a plurality of reaction vessel positions in the inner three circles, and the outer ring does not require an additional reaction vessel position for the incubation, thereby making the reaction tray smaller in size and lower in cost. .
- test reaction container needs to be incubated for 25 minutes, it can be done for most of the time, such as 24 minutes, on one or several turns of the inner three rings 11a, 11b, and 11c. Transfer to the outer ring 11d and complete the remaining 1 minute incubation before transferring to the wash separation unit. Because this kind of scheme shares the function of partial incubation, the number of reaction vessels in the inner three rings can be appropriately reduced, so that the number of reaction vessels in the inner and outer rings can be balanced, thereby optimizing the size of the reaction disk and making full use of the internal space of the reaction disk. .
- Step 200 loads the reaction vessel: Transfer unit 50 transfers an unused reaction vessel from reaction vessel supply unit 70 to the reaction vessel location of fill station 90.
- the step 201 is to fill the sample and the reagent: the filling unit 20 respectively sucks the sample and the reagent from the suction sample position and the suction reagent position into the reaction container on the filling station 90.
- Step 202 Mixing: If mixing is required, the mixing mechanism mixes the sample and reagents in the reaction vessel. If no mixing is required, this step is omitted.
- Step 203 The transfer unit 50 transfers the reaction container filled with the sample and the reagent from the filling station 90 to the reaction tray 11 by incubating the transfer position (one of 12a 1-3 , 12b 1-3 , 12c 1-3 ) Within one of the three reaction chambers 11a, 11b, 11c, the reaction vessel begins to incubate on the reaction tray. While the reaction vessel is being incubated, the reaction disk 11 is rotated one position at a fixed time. The incubation time varies depending on the specific test item, typically 5 to 60 minutes.
- Step 204 Washing and separating: After the incubation is completed or after a certain period of incubation, the transfer unit 50 passes the transfer position (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) from the reaction tray 11 three times 11a The reaction vessel of 11b or 11c is displaced, and the reaction vessel is moved to the reaction vessel position of the outer ring 11d of the reaction disk 11 by washing and separating the transfer sites (13d 1 or 13d 2 ). The reaction disk 11 is rotated one position at a fixed time, and the reaction container on the outer ring 11d reaction container position is transferred to the cleaning and separating device 16. If the reaction vessel has been incubated, no incubation is required on the outer ring 11d during the transfer.
- reaction vessel incubation is not completed, the remaining time incubation is completed during the transfer to the wash separation device 16.
- the washing mechanism and the mixing mechanism of the washing and separating device 16 complete the liquid absorption, the washing buffer, and the washing and mixing until the cleaning separation is completed. .
- Step 205 is filled with a signal reagent: after the cleaning separation is completed, the reaction tray 11 is transferred to the outer ring 11d. The reaction container at the reaction container is separated from the magnetic field region, and the signal reagent injection mechanism coupled by the cleaning and separating mechanism injects all or part of the reaction container into the reaction container. Signal reagent.
- Step 206 Signal Incubation: If signal incubation is desired, signal incubation is accomplished during transfer of the reaction vessel 11 to the reaction vessel on the outer race 11d to the measurement device 86. This step is omitted if no signal incubation is required.
- Step 207 The reaction container to be measured is transferred to the measuring unit 86 on the outer ring 11d, and if necessary, all or part of the signal reagent is injected, and the reaction signal in the reaction container is measured by the measuring device 86, and the measurement result is processed. It is then sent to the control center of the automatic analyzer.
- Step 208 discards the reaction vessel: the reaction tray 11 continues to transfer the reaction vessel on the outer ring 11d to the washing separation transfer position (13d 1 or 13d 2 ), and the transfer unit 50 removes the measured reaction vessel from the reaction tray and transfers to the discarded reaction vessel. Hole 60 is discarded.
- the one-step one-step test procedure and procedure differs from the one-step test in that steps 301-305 are used to separate the reagents and add one incubation.
- the other steps are similar to the one-step method. ,No longer.
- Step 301 fills the sample and the first reagent: the filling unit 20 injects the sample and the first reagent from the suction sample position and the suction reagent position into the reaction container on the filling station 90, respectively.
- Step 302 Mix well: If mixing is required, if mixing is required, the mixing mechanism feeds the sample and reagents in the reaction vessel. Mix well. If no mixing is required, this step is omitted.
- Step 303 First incubation: Transfer unit 50 transfers the reaction container filled with sample and reagent from the filling station 90 through the incubation transfer position (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) to One of the three reaction chambers 11a, 11b, 11c has a reaction vessel position, and the reaction vessel begins to incubate on the reaction tray. While the reaction vessel is being incubated, the reaction disk 11 is rotated one position at a fixed time. The incubation time varies depending on the specific test item, typically 5 to 60 minutes.
- Step 304 filling the second reagent: after the first incubation is completed, the transfer unit 50 passes the reaction vessel through the incubation transfer position (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) from the reaction tray 11 The reaction vessel locations on the loops 11a, 11b, 11c are transferred to the reaction vessel location on the fill station 90, and the fill unit 20 draws the second reagent from the draw reagent station into the reaction vessel on the fill station 90.
- the transfer unit 50 passes the reaction vessel through the incubation transfer position (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) from the reaction tray 11
- the reaction vessel locations on the loops 11a, 11b, 11c are transferred to the reaction vessel location on the fill station 90, and the fill unit 20 draws the second reagent from the draw reagent station into the reaction vessel on the fill station 90.
- step 305 If mixing is required, if mixing is required, the mixing mechanism mixes the sample and reagents in the reaction vessel. If no mixing is required, this step is omitted.
- step 404 is added to add a wash separation.
- Step 404 Washing separation: After the first incubation is completed or after the first incubation for a certain period of time, the transfer unit 50 removes the reaction vessel from the reaction by incubating the transfer sites (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) The reaction vessel of the three turns 11a, 11b or 11c in the tray 11 is displaced, and the reaction vessel is moved into the reaction vessel position of the outer ring 11d of the reaction disk 11 by the washing separation transfer position (13d 1 or 13d 2 ). The reaction disk 11 is rotated one position at a fixed time, and the reaction container on the outer ring 11d reaction container position is transferred to the cleaning and separating device 16.
- the transfer unit 50 passes the reaction vessel through the incubation transfer position (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) from the three turns 11a, 11b, 11c in the reaction disk 11.
- the reaction vessel position is transferred to a reaction vessel on the filling station 90, and the filling unit 20 draws a second reagent from the aspirating reagent site into the reaction vessel on the filling station 90.
- the automatic analysis device 100 is first concentrated or incubated for a certain period of time in the inner three rounds, and the reaction container that has been incubated or incubated for a certain period of time is transferred to the outer ring for the remaining time to complete the cleaning and separation. And measuring, the transfer of the reaction vessel between different circles is completed by the transfer unit through at least one incubation transfer position and one cleaning separation transfer position disposed on the reaction unit, which not only saves the independent cleaning separation disk and the optical disk used in the prior art. , reducing the size of the machine and reducing the cost, but also streamlining the test steps and reducing the complexity and difficulty of the control, avoiding the transfer of the reaction vessel between multiple disks.
- reaction unit can adjust, set and balance the number of reaction vessels in the inner and outer ring by setting different transfer positions, which not only can realize flexible incubation time, but also fully utilize the internal space of the reaction disk, thereby further reducing the reaction unit.
- the size makes the whole structure more compact, lower cost and more efficient.
- FIG. 1 A second embodiment of the invention is shown in FIG.
- the sample transport unit 30, the reagent storage unit 40, and the filling unit 20 in this embodiment are the same as or similar to those in the first embodiment, and will not be described again.
- the transfer unit 50 is set to one, which can perform two-dimensional motion, which makes the whole machine more compact and lower in cost.
- the transfer unit 50 includes a Y-direction guide rail 50a, a Y-direction moving robot arm 50b, and a mechanism such as a vertical motion mechanism and a mechanical finger (not shown).
- the transfer unit 50 can move the mechanical finger horizontally along the Y direction, the horizontal movement range is a one-dimensional linear region 56, and the two reaction transfer positions (13b, 13c) of the reaction container on the reaction container supply unit 70 and the reaction unit 10 can be One wash separation transfer position (13a) on the reaction unit 10, one measurement transfer position (13d) on the reaction unit 10, and a lost reaction container position 60 are transferred.
- the transfer unit since the range of motion of the transfer unit 50 covers a plurality of incubation transfer sites on the reaction unit 10, the transfer unit can be transferred into the reaction vessel through different incubation shifts or transferred from the different incubation transfer positions to achieve flexible incubation. Breeding time.
- the main difference between the filling station 90 and the first embodiment is that it can move horizontally and can move horizontally along the X direction to the horizontal range of motion of the transfer unit 50.
- the main difference between the reaction vessel supply unit 70 and the first embodiment is that only one column of the reaction vessel thereon is in the horizontal movement range of the transfer unit 50. In order to continuously supply the reaction vessel, the reaction vessel supply unit 70 can move horizontally along the X direction. So that the columns of reaction vessels above it pass the horizontal range of motion of the transfer unit 50, such that the transfer unit 50 can traverse the unused reaction vessels on each of the reaction vessel locations on the tray to provide an unused reaction for the newly initiated test. container.
- the main difference between the reaction unit and the first embodiment is in the arrangement of the cleaning separation device and the setting of the transfer position.
- the cleaning and separating device 16 is disposed in the inner ring 11a of the reaction disk, and cleans and separates the reaction container that has entered the reaction disk inner ring 11a by the cleaning separation transfer position 13a.
- the measuring device 86 is mounted on the side of the heat retaining device and measures the signal in the reaction vessel that has entered the outer ring 11d of the reaction disk by measuring the transfer position 13d.
- the reaction vessel positions on the middle two turns 11b, 11c are incubated with the reaction vessel entering the treatment unit by incubation of the transfer sites (13b, 13c).
- the cleaning separation transfer bit 13a is sequentially disposed from the inside to the outside on the reaction unit.
- the transfer sites 13b and 13c were incubated, and the transfer sites 13d were measured for a total of 4 transfer sites.
- the cleaning and separating device is arranged on the inner ring of the reaction unit, which not only makes the cleaning and separating device more compact, but also reduces the adverse effects of the cleaning and separating device on the temperature fluctuations that may be caused by the measurement and the interference of introducing ambient light.
- the filling station 90 is moved horizontally in the X direction to the horizontal movement range of the transfer unit 50, and the transfer unit 50 transfers an unused reaction container from the reaction container supply unit 70 to the reaction container position of the filling station 90, and then adds
- the filling station 90 moves to the horizontal movement track of the filling unit 20, and the filling unit 20 fills the reaction container on the filling station 90 with the sample and the reagent.
- the mixer integrated in the filling station 90 can be Mix the reaction vessel.
- the filling station 90 is again horizontally moved to the horizontal movement range of the transfer unit 50.
- the reaction vessel to be incubated on the filling station 90 is first transferred by the transfer unit 50 through the incubation transfer position 13b or 13c into one of the middle two loops 11b, 11c, and after the incubation is completed or after a certain period of time, it is necessary to wash and separate and then transfer.
- the unit 50 moves out of the middle two rings 11b, 11c by the incubation transfer position 13b or 13c, and then moves into the inner ring 11a through the cleaning separation transfer position 13a, and is cleaned and separated by the cleaning and separating device 86 under the rotation transfer of the reaction disk, and the cleaning and separation are completed.
- the transfer unit 30 removes the inner ring 11d by the cleaning separation transfer position 13a. If the second reagent needs to be added, the transfer unit 50 transfers the reaction container to the filling station 90 to complete the filling of the second reagent; if measurement is required, The transfer unit 50 is moved into the outer ring 11d by measuring the transfer position 13d, and the reaction container is transferred to the measuring device for measurement under the rotation of the reaction disk.
- FIG. This embodiment differs from the first embodiment in the arrangement of the measuring device.
- This embodiment also includes measuring a darkroom (not shown) and a measurement location 82 independent of the reaction unit 10, and the measurement device 86 is mounted on the measurement darkroom to measure the signal in the reaction vessel on the measurement site 82.
- the measurement darkroom provides the desired darkroom environment for the measurement device 86, which is within the horizontal range of motion of the transfer unit 50 or can be moved horizontally to the horizontal range of motion of the transfer unit 50.
- the measuring position 82 can be made into a fixed position, and the inlet and outlet of the reaction container are provided with a "sunroof" mechanism, which is normally closed to ensure the measurement of the darkroom environment of the darkroom, and the reaction container is opened when it enters and exits; the measuring position 82 can also be made mobile.
- the position, in order to be easily protected from light, the measuring position 82 can be moved away from or close to the measuring device 86 in the form of a push-pull drawer or the like.
- the measurement bit 82 and the corresponding light-shielding structure may be other suitable implementations.
- the filling of the signal reagent can also be done at measurement position 82.
- This embodiment makes the measuring device 86 relatively independent, and it is easier to realize a closed darkroom environment during measurement, and the reaction unit does not need to be provided with a structure specifically for the light-shielding requirement of the measuring device 86. It will be understood by those skilled in the art that other units of the present embodiment are the same as or similar to the first embodiment.
- the test flow and steps of this embodiment refer to FIG. 5, FIG. 6, and FIG. 7, which are mainly different from the first embodiment. Fill the signal reagent, measure, and discard the reaction vessel in three steps, the same or similar.
- the step of filling the signal reagent in the embodiment may be completed on the reaction vessel position of the outer ring 11d of the reaction disk, or may be completed at the measurement position 82, and the first signal reagent may be completed on the reaction vessel position of the outer ring 11d of the reaction disk.
- the transfer unit 50 transfers the reaction container to be measured through the cleaning separation transfer position 13d 1 or 13d 2 from the reaction container position of the outer disk 11d of the reaction disk
- the reaction signal in reaction vessel located at measurement location 82 is measured by measurement device 86; the reaction vessel step is discarded, and transfer unit 50 transfers the measurement vessel from which measurement is completed from measurement location 82 to disposal aperture 60.
- FIG. 1 differs from the first embodiment in that it further includes a measuring dark chamber 82 and a measuring disk 81 which are independent of the reaction unit 10, and the measuring device 86 is mounted on the measuring dark room 82.
- the filling station (not shown) can be integrated in the measuring disc 81, and the reaction vessel position of the measuring disc 81 and its rotational positioning function can be fully utilized, so that the independent filling station can be omitted, saving
- the mechanism can make the whole machine cost less and the structure is more compact.
- the mixing mechanism can be integrated into the filling station for ultrasonic mixing or shaking mixing of the filled reaction vessel.
- a measuring reaction vessel position 81a is provided on the measuring disk 81 to measure the center of rotation of the disk for carrying the reaction vessel to be measured.
- a plurality of reaction vessel positions are provided, and all or part of the signal incubation can be achieved.
- the measurement transfer position 82a is set in the upper portion of the measurement dark room 82.
- the measurement transfer position 82a is within the horizontal movement range of the transfer unit 50, and the transfer unit 50 can remove the reaction container to be measured from the reaction disk 11 through the cleaning separation transfer position 13d 1 or 13d 2 of the reaction unit 10, and move it in by the measurement transfer position 82a.
- the disc 81 is measured.
- the measurement dark chamber 82 is wrapped or surrounded by the periphery of the measuring disc 81 to provide a darkroom environment for the measuring device 86.
- the heating device and the sensor may be selectively disposed on the side or the bottom of the measuring dark chamber 82 to provide a constant temperature for the measuring disc reaction container position 81a. Signal incubation environment.
- the measuring device 86 includes a weak photodetector photomultiplier tube (PMT) directly mounted on the measuring dark chamber 82 to measure a weak chemiluminescence signal generated by adding a signal reagent to the reaction vessel.
- PMT weak photodetector photomultiplier tube
- the upper portion of the measuring disk 81 of the present invention or the periphery of the measuring dark room 82 may be provided with a signal reagent filling mechanism (not shown) to react to the reaction position of the measuring disk 81. Fill all or part of the signal reagent in the container. It will be understood by those skilled in the art that other units of the present embodiment are the same as or similar to the first embodiment.
- the test flow and steps of the present embodiment are different from those of the first embodiment with reference to FIG. 5, FIG. 6, and FIG.
- Loading the reaction vessel, filling the sample and reagents, and finally adding the signal reagent, measuring, discarding the reaction vessel, and the like, the others are the same or similar.
- the transfer unit 50 moves the unused reaction container from the reaction container supply unit 70 through the measurement transfer position 82a to the reaction container position on the measuring disk 81, the measurement disk 81 is rotated, the reaction container is transferred to the filling station, and the filling unit 20 sucks the sample.
- the reagent is filled into the reaction vessel at the filling station. After the filling is completed, the mixing mechanism integrated in the filling station mixes the mixture in the reaction vessel.
- the transfer unit 50 transfers the measurement container that has completed the measurement from the reaction vessel position on the measurement disk 81 to the reaction unit by measuring the transfer position 82a.
- the step of filling the signal reagent in the embodiment may be completed at the reaction vessel position of the outer ring 11d of the reaction disk, or may be completed at the reaction vessel position on the measuring disk 81, or may be at the reaction vessel position of the outer ring 11d of the reaction disk.
- the filling of the first signal reagent is completed, and the filling of the second signal reagent is completed in the reaction container position on the measuring disk 81; in the measuring step, the transfer unit 50 passes the reaction container to be measured through the cleaning separation transfer position 13d1 or 13d2 from the reaction disk.
- the reaction vessel of the outer ring 11d is displaced, moved into the reaction vessel position on the measuring disk 81 by the measuring transfer position 82a, the measuring disk 81 is rotated, and the reaction vessel is transferred to the measuring device 86, and the reaction signal in the reaction vessel is performed by the measuring device 86.
- the reaction vessel step is discarded, and the transfer unit 50 transfers the measurement container that has completed the measurement from the reaction vessel position on the measuring disk 81 to the disposal hole 60 by the measurement transfer position 82a.
- the automatic analysis device of the invention can also be flexibly expanded and maximized to achieve serialization of products.
- the number of transfer units and filling units can be increased, the size of the reaction unit can be appropriately increased, or the reaction can be increased.
- the number of units is used to achieve this.
- FIG 11 there is shown a schematic view of a fifth embodiment of the automatic analyzer of the present invention.
- the sample transport unit 30 adopts the injection mode of the track and the sample rack, so that more samples can be accommodated, the sample can be added in real time, and the operation is more convenient.
- the sample holder 32 and the sample tube 31 thereon can be delivered to the range of motion of the first filling unit 21.
- the reagent storage unit 40 increases the reagent storage position and allows more reagent containers to be placed.
- the filling unit 20 includes a first filling unit 21 and a second filling unit 22, the first filling unit 21 only filling the sample or filling the sample and the partial reagent, the second The filling unit 22 is filled with reagents, and of course more filling units can be added, which increases the speed of adding the sample and the reagent.
- the reaction vessel supply unit 70 adopts a silo type, and the reaction vessel can be poured into a silo of the reaction vessel supply unit 70 in a scattered manner, which makes the supply of the reaction vessel more, faster, and more convenient.
- the reaction disk 11 of the reaction unit 10 includes an outer ring 11d reaction vessel position and an inner region 11a reaction vessel position distributed centering on the center of rotation of the reaction disk.
- the reaction vessel position on the inner region 11a is distributed in a "honeycomb" manner, so that the space on the reaction disk 11 can be fully utilized, more reaction vessel positions can be set, more reaction vessels can be accommodated, and the test throughput can be increased.
- an incubation transfer zone 13a (including 7 incubation transfer sites) and a wash separation transfer site 13d are disposed on the reaction unit 10.
- the measurement dark room 82 and the measuring disk 81 and the measuring device 86 can be completely multiplexed with the fourth embodiment, but in order to improve the test efficiency, the filling bit is no longer set.
- a measurement transfer position 82a is provided on the measurement dark chamber 82 for the reaction container to enter and exit the measurement disk.
- the transfer unit 50 includes a first transfer unit 51 and a second transfer unit 52 that can perform three-dimensional movement independently.
- the first reaction container unit 51 is mainly in the incubation transfer zone 13a of the reaction unit 10, the cleaning separation transfer position 13d, the measurement disk 81, and the reaction.
- the reaction container is transferred between the container discarding holes 60b and the like, and the second transfer unit 52 is mainly at the reaction container supply unit 70, the filling station 90, the incubation transfer zone 13a of the reaction unit 10, and the cleaning separation transfer position 13d and the reaction container disposal hole 60b. Transfer the reaction vessel between.
- the transfer of the reaction vessel between any two locations can be accomplished simultaneously by the first or second transfer unit or both. Of course, there may be more than two transfer units, and more transfer units may be provided as needed to increase the efficiency and speed of transfer of the reaction vessel.
- the present embodiment uses the method of the independent filling station 90 to fill the sample and the reagent.
- the filling station 90 can move back and forth between the reaction container supply unit 70, the first filling unit 21, and the second filling unit 22, receive the reaction container supplied from the reaction container supply unit 70, and accept the filling of the first filling unit 21.
- the sample or sample and a portion of the reagent are received by the second filling unit 22.
- the mixing mechanism can be integrated on the filling station 90 or the filling unit 20 to mix the reaction container after the sample or/and the reagent is added. After the mixing is completed, the reaction vessel on the filling station 90 is transferred from the transfer unit 50 to the reaction unit 10.
- test procedure and steps of the present embodiment are mainly different from the first embodiment in that the sample and reagent are filled by the first and second filling.
- the unit coordination is completed, the reaction container transfer is completed by the first and second transfer units, and the filling operation of the filling unit is completed at the independent filling station.
- the other actions and processes are the same or similar to those of the first embodiment. - Figure 7, no further details.
- this embodiment avoids the extra large size of the cleaning separation disc, and the measurement unit independent of the reaction unit is easier to realize the darkroom environment and flexible measurement, and the partition of the reaction vessel position through different functions is also The size of the reaction unit itself is reduced, making the machine more compact, less costly, more efficient and more reliable.
- the embodiment of the invention further provides a sample analysis method, which specifically includes:
- washing and separating step of washing and separating the reaction vessel entering the reaction unit through at least one washing and separating transfer point to remove unbound components in the reactant;
- the measuring step measures the reaction signal in the reaction vessel by the measuring device.
- the invention realizes the incubation and washing separation of the reactants in the reaction vessel centering on the reaction unit, and at least one incubation transfer position and at least one cleaning separation transfer position are arranged on the reaction unit, and the transfer unit can be transferred between the incubation transfer position and the cleaning separation position.
- the reaction vessel can not only realize flexible incubation, but also solve the problem that the two-step test must be implemented by using multiple cleaning and separating mechanisms in the prior art, and fully realize efficient cleaning separation.
- the measuring device can be The layout or structure of the whole machine needs to be flexibly arranged or arranged, for example, it can be directly installed on the reaction unit, set in a separate position or installed on an independent measuring plate, etc., which solves the limitation of the arrangement of the measuring device in the prior art and the measurement.
- the environment is prone to interference and other issues.
- the invention improves the working efficiency of the analysis device and reduces the difficulty of realizing the automatic function, and solves the technical problems of large volume, low detection speed, high cost and poor performance of the current automatic instrument, which not only saves the laboratory space, but also improves the laboratory space. Test efficiency, and help reduce expenses, reduce the burden on the testee, and ultimately save a lot of natural resources and social resources.
- Various embodiments may be included in various embodiments of the invention, which may be embodied as machine-executable instructions that are executable by a general purpose or special purpose computer (or other electronic device). Alternatively, these steps may be performed by hardware elements comprising specific logic circuitry to perform the steps or jointly by hardware, software and/or firmware.
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Abstract
An automatic analyzer and a sample analysis method. The automatic analyzer (100) comprises: a filling unit (20) for filling a reaction vessel with a sample and/or a reagent; a reacting unit (10) for incubating, cleaning, and separating reactants in the reaction vessel; a measuring apparatus (86) for measuring a reaction signal in the reaction vessel; and a transferring unit (50) for transferring the reaction vessel between different positions. At least one incubation transfer position (13a1-3, 13b1-3, and 13c1-3) and at least one cleaning and separating transfer position (13d1-2) are provided on the reacting unit (10). The transferring unit (50) is capable of transferring the reaction vessel between the incubation transfer position (13a1-3, 13b1-3, and 13c1-3) and the cleaning and separating position (13d1-2). Not only flexible incubation can be implemented, but the problem in the prior art in which multiple cleaning and separating mechanisms must be employed to implement a two-step test is also solved, and efficient cleaning and separation are fully implemented.
Description
本发明涉及体外诊断设备领域,具体涉及一种自动分析装置及样本分析方法。The invention relates to the field of in vitro diagnostic equipment, in particular to an automatic analysis device and a sample analysis method.
近年来,临床检验和自动化技术的发展和进步,不仅提升了临床实验室自动化水平,提高了医学检验的效率,也改善了检验结果的质量和可靠性。然而,随着检测标本量的增多,临床实验室需要不断增添大型自动化检测系统以满足其检测需求,从而导致实验室日益拥挤和检测成本不断攀升。因而,如何在面临医保控费的压力和挑战下,提升检验效率、保证结果并充分利用现有实验室资源和减少检测成本支出,是临床检验要解决的一个迫切问题。In recent years, the development and advancement of clinical testing and automation technology has not only improved the level of clinical laboratory automation, but also improved the efficiency of medical testing and improved the quality and reliability of testing results. However, as the number of specimens is increased, clinical laboratories are continually adding large automated inspection systems to meet their testing needs, resulting in increasing laboratory congestion and rising testing costs. Therefore, how to improve the inspection efficiency, ensure the results and make full use of existing laboratory resources and reduce the cost of testing under the pressure and challenge of medical insurance control is an urgent problem to be solved by clinical testing.
为了表述方便,本文以体外诊断(In-Vitro Diagnostics,简称IVD)中的全自动免疫分析仪,特别地,以发光免疫分析仪为列,阐述本技术方案和方法,本领域内技术人员应该理解,本发明方案和方法也可用于其它临床检验自动化装置,比如荧光免疫装置、电化学免疫等。全自动免疫分析通过以抗原抗体相互结合的免疫学反应为基础,使用酶标记、镧系元素标记或化学发光剂标记抗原抗体,通过一系列级联放大反应,将光信号或电信号与分析物浓度等相联系,分析人体样本中的待测的抗原或抗体,主要应用于医院的检验科、第三方独立实验室、血检中心等机构,对人类体液中的各分析物含量进行定量、半定量或定性检测,进行传染病、肿瘤、内分泌功能、心血管疾病和优生优育以及自身免疫类疾病等的诊断。全自动免疫分析仪通常由取样单元、反应单元、供应和废物废液单元、系统控制单元等组成。发光免疫由于具有定量检测、灵敏度高、特异性好、线性范围宽、自动化程度高等优势正成为目前自动化免疫的主流技术。全自动发光免疫分析根据标记方法和发光体系不同,又包括酶促化学发光、直接化学发光、电化学发光等。For convenience of description, the present invention is described in the context of a fully automated immunoassay instrument in In-Vitro Diagnostics (IVD), in particular, a luminescent immunoassay analyzer, which should be understood by those skilled in the art. The protocols and methods of the present invention are also applicable to other clinical test automation devices, such as fluorescent immuno devices, electrochemical immunization, and the like. Automated immunoassay based on immunological reactions in which antigen and antibody bind to each other, labeling antigen antibodies with enzyme labels, lanthanide labels or chemiluminescent agents, and optical or electrical signals and analytes through a series of cascade amplification reactions The concentration is related to the analysis of the antigen or antibody to be tested in the human sample, and is mainly used in hospitals, third-party independent laboratories, blood test centers, etc., to quantify the content of each analyte in human body fluids. Quantitative or qualitative testing for the diagnosis of infectious diseases, tumors, endocrine functions, cardiovascular diseases, prenatal and postnatal diseases, and autoimmune diseases. The automatic immunoassay analyzer usually consists of a sampling unit, a reaction unit, a supply and waste waste unit, a system control unit, and the like. Luminescence immunity has become the mainstream technology of automatic immunity due to its advantages of quantitative detection, high sensitivity, good specificity, wide linear range and high degree of automation. The fully automatic luminescence immunoassay differs according to the labeling method and the luminescence system, and includes enzymatic chemiluminescence, direct chemiluminescence, and electrochemiluminescence.
参考附图1-3,发光免疫分析按测试原理和模式一般可分为一步法、延时一步法、两步法等,主要测试步骤一般包括加注样本和试剂、反应物混匀、孵育、清洗分离(Bound-Free,简称B/F)、加信号试剂、测量等。需要指出的是,为了表述方便,本发明区分了试剂和信号试剂、孵育和信号孵育。试剂与分析项目为“一一对应”关系,即不同分析项目对应的具体试剂在配方、试剂量、组分数量等方面一般不同。根据具体分析项目的不同,试剂通常包括多个组分,如常见的2-5个组分,包括磁微粒试剂、酶标试剂、稀释液等试剂组分。根据反应模式不同,一个分析项目的多个试剂组分可以一次性加注也可以分多个步骤加注,分步骤加注时按照加注次序定义为第一试剂、第二试剂、第三试剂等。信号试剂用于测量信号的产生,通常为通用试剂的一种,与分析项目为“一对多”的对应关系,即不同的分析项目共用信号试剂。本发明的孵育特指反应容器开始清洗分离前,其内的反应物在反应单元的恒温环境下发生的抗原抗体结合反应或生物素亲和素结合反应的过程,具体地,一步法孵育一次,为进入清洗分离前的一次孵育,延时一步法孵育两次,包括加注第二试剂前的第一次孵育和进入清洗分离前的第二次孵育,两步法孵育两次,包括第一次清洗分离前的第一次孵育和第二次清洗分离前的第二次孵育。而信号孵育指清洗分离后的反应容器在加入信号试剂后,在恒温环境下反应一段时间,使信号增强的过程。根据反应体系和发光原理的不同,并不是所有测试都需要信号孵育,需要信号孵育的测试一般为酶促类化学发光免疫分析。不同测试模式对应的测试步骤详述如下:Referring to Figures 1-3, the luminescence immunoassay can be generally divided into a one-step method, a one-step one-step method, a two-step method, etc. according to the test principle and mode. The main test steps generally include adding a sample and a reagent, mixing the reactants, and incubating. Cleaning separation (Bound-Free, referred to as B/F), adding signal reagents, measuring, etc. It should be noted that the present invention distinguishes between reagents and signal reagents, incubation and signal incubation for ease of presentation. The reagents and analysis items have a “one-to-one correspondence” relationship, that is, the specific reagents corresponding to different analysis items generally differ in formula, reagent amount, and component quantity. Depending on the specific analytical item, the reagent typically includes multiple components, such as the usual 2-5 components, including reagent components such as magnetic particle reagents, enzyme labeling reagents, diluents, and the like. Depending on the reaction mode, multiple reagent components of an analysis item can be filled in one time or in multiple steps. When the step is added, the first reagent, the second reagent, and the third reagent are defined according to the order of filling. Wait. The signal reagent is used to measure the generation of the signal, usually a kind of general-purpose reagent, and the analysis item is "one-to-many" correspondence, that is, different analysis items share the signal reagent. The incubation of the present invention specifically refers to the process of antigen-antibody binding reaction or biotin-avidin binding reaction of the reactants in the constant temperature environment of the reaction unit before the start of washing and separation, specifically, one-step incubation, To enter an incubation prior to wash separation, delay the incubation by one step in a single step, including the first incubation before the second reagent is added and the second incubation before the wash separation, and the two-step incubation twice, including the first The first incubation before the separation is separated and the second incubation before the second cleaning separation. The signal incubation refers to a process in which the reaction vessel after the separation and separation is added to the signal reagent and reacted in a constant temperature environment for a period of time to enhance the signal. Depending on the reaction system and the principle of luminescence, not all tests require signal incubation, and tests that require signal incubation are typically enzymatic chemiluminescence immunoassays. The test steps corresponding to different test modes are detailed as follows:
1)一步法:参考附图1,加注样本(S)和试剂(R),混匀(有些测试方法也可以不需要混匀,下同,不再赘述),孵育(一般为5-60分钟),孵育完成后进行清洗分离,加注信号试剂,信号孵育(一般为1-6分钟),最后测量。需要指出的是,由于信号试剂具体成分的不同,有些发光体系不需要信号孵育,在加注信号试剂过程中或加注完信号试剂
后可以直接测量。信号试剂可以是一种或多种,参考附图2,信号试剂包括第一信号试剂、第二信号试剂。1) One-step method: Refer to Figure 1, add sample (S) and reagent (R), mix (some test methods can also do not need to mix, the same below, no longer repeat), incubate (generally 5-60 Minutes), after the incubation is completed, wash and separate, add signal reagent, signal incubation (usually 1-6 minutes), and finally measure. It should be pointed out that some luminescent systems do not require signal incubation due to the specific composition of the signal reagents. During the filling of the signal reagents or after the filling of the signal reagents
It can be measured directly afterwards. The signal reagent may be one or more. Referring to Figure 2, the signal reagent includes a first signal reagent and a second signal reagent.
2)延时一步法:与一步法不同之处在于试剂分两次加注、需要两次孵育,加第一试剂混匀后进行第一次孵育,第一次孵育完成后加第二试剂并混匀。与一步法相比多了一次孵育、加注试剂和混匀动作,其余流程与一步法一样。2) One-step delay method: the difference from the one-step method is that the reagent is added twice, and requires two incubations. After the first reagent is mixed, the first incubation is performed, and after the first incubation, the second reagent is added. Mix well. One more incubation, reagent addition, and mixing action than the one-step method, and the rest of the procedure is the same as the one-step method.
3)两步法:与延时一步法不同在于多了一次清洗分离步骤,其它步骤相同。3) Two-step method: The difference from the one-step method is that there is one more cleaning and separation step, and the other steps are the same.
为了实现上述流程自动化测试,现有的具体实现技术方案如下:In order to achieve the above process automation test, the existing specific implementation technical solutions are as follows:
第一种现有技术方案将孵育、清洗分离和测量分开独立布局,分别由三个旋转圆盘完成相应功能,反应容器在不同单元之间由机械抓臂完成转移。该技术方案组件和单元多,孵育转移位、清洗分离转移位和测量转移位分散在不同的圆盘上,且距离远,反应容器需要在各转移位之间转移,造成体积大、成本高、运动路径多、控制流程复杂等问题。The first prior art solution separates the incubation, wash separation, and measurement into separate layouts, each of which is accomplished by three rotating disks, and the reaction vessel is transferred between the different units by a mechanical gripper. The technical scheme has many components and units, the incubation transfer position, the cleaning separation transfer position and the measurement transfer position are dispersed on different discs, and the distance is long, and the reaction container needs to be transferred between the transfer positions, resulting in large volume and high cost. There are many problems such as many moving paths and complicated control processes.
第二种现有技术方案将孵育和测量布置在一起构成孵育测量单元,清洗分离由另一个独立单元完成,虽然与第一种现有方案相比,该技术方案减少了一个测量圆盘,在一定程度上有利于控制整机尺寸和成本,但同样存在与第一种技术方案相同的问题。该技术方案为了实现灵活的孵育时间,孵育测量单元控制复杂,孵育和测量在控制上也会相互制约,不仅存在无法实现高速自动化测试等缺点,也无法实现灵活的信号孵育。A second prior art solution arranges the incubation and measurement together to form an incubation measurement unit, which is performed by another separate unit, although the technical solution reduces one measurement disc compared to the first prior scheme. To a certain extent, it is advantageous to control the size and cost of the whole machine, but there are also the same problems as the first technical solution. In order to achieve flexible incubation time, the incubation measurement unit is complicated to control, and the incubation and measurement are also controlled by each other. There are not only shortcomings such as high-speed automated testing, but also flexible signal incubation.
第三种现有技术方案将孵育、清洗分离和测量在一个单圈圆盘或歧形轨道上实现,该方案为了支持较长的孵育时间,圆盘除了清洗分离和测量位置外,还需要设置很多的孵育位置,这样为了实现高速测试,圆盘或歧形轨道尺寸需要设计得很大,生产制造难度大、成本高,此外,为了实现延时一步法和两步法测试,还需要至少两个加样机构和至少两个清洗分离机构,从而增加了物料、加工、生产成本和整机尺寸。另一方面,该技术方案还限制了孵育时间,导致了孵育时间固定、出结果时间过长等问题。此外,该技术方案不仅很难实现测量所需的暗室环境,需要增加额外的快门机构,还无法实现灵活的信号孵育。A third prior art solution achieves incubation, wash separation and measurement on a single-turn disc or a trajectory track. In order to support longer incubation times, the disc needs to be set in addition to cleaning separation and measurement positions. A lot of incubation positions, so in order to achieve high-speed testing, the size of the disc or the trajectory track needs to be designed to be large, difficult to manufacture, and costly. In addition, in order to achieve the one-step and two-step test, at least two A loading mechanism and at least two cleaning separation mechanisms increase material, processing, production costs and overall machine size. On the other hand, this technical solution also limits the incubation time, resulting in problems such as a fixed incubation time and an excessively long time. In addition, this technical solution is not only difficult to achieve the darkroom environment required for measurement, it requires an additional shutter mechanism, and flexible signal incubation is not possible.
发明内容Summary of the invention
为解决现有技术普遍存在的缺点和问题,本发明提供一种生产制造成本低、结构简单紧凑、测试流程或方法灵活高效的自动分析装置及样本分析方法。In order to solve the shortcomings and problems common in the prior art, the present invention provides an automatic analysis device and a sample analysis method with low manufacturing cost, simple and compact structure, flexible and efficient test procedure or method.
根据本发明的一方面,提供一种自动分析装置,包括:加注单元,加注样本和/或试剂到反应容器,反应单元,孵育并清洗分离反应容器内的反应物,测量装置,测量反应容器内的反应信号,转移单元,在不同位置之间转移反应容器,所述反应单元包括一个旋转装置,所述旋转装置上设置若干个反应容器位,用于承载和固定反应容器,所述反应单元上设置至少一个孵育转移位和至少一个清洗分离转移位,所述反应单元的至少一个孵育转移位和至少一个清洗分离转移位在转移单元的水平运动范围内。According to an aspect of the invention, there is provided an automatic analysis device comprising: a filling unit, filling a sample and/or a reagent into a reaction vessel, a reaction unit, incubating and washing the reactants in the separation reaction vessel, measuring the device, measuring the reaction a reaction signal in the vessel, a transfer unit, transferring the reaction vessel between different positions, the reaction unit comprising a rotating device, the rotating device is provided with a plurality of reaction vessel positions for carrying and fixing the reaction vessel, the reaction At least one incubation transfer site and at least one wash separation transfer site are disposed on the unit, the at least one incubation transfer site of the reaction unit and the at least one wash separation transfer site being within a horizontal range of motion of the transfer unit.
根据本发明的另一方面,提供一种样本分析方法,包括:加注步骤,在反应容器中加注样本和/或试剂,孵育步骤,对通过至少一个孵育转移位进入反应单元的反应容器进行孵育,清洗分离步骤,对通过至少一个清洗分离转移位进入反应单元的反应容器进行清洗分离,以去除反应物中未结合的成分,加注信号试剂步骤,向反应容器内加注信号试剂,测量步骤,通过测量装置对反应容器内的反应信号进行测量。According to another aspect of the present invention, there is provided a sample analysis method comprising: a filling step of filling a sample and/or a reagent in a reaction vessel, and an incubating step of performing a reaction vessel entering the reaction unit through at least one incubation transfer position Incubating, washing and separating the step, washing and separating the reaction vessel entering the reaction unit through at least one washing and separating transfer position, removing unbound components in the reactant, adding a signal reagent step, adding a signal reagent to the reaction container, and measuring In the step, the reaction signal in the reaction vessel is measured by a measuring device.
本发明以反应单元为中心实现反应容器内反应物的孵育、清洗分离,反应单元上设置至少一个孵育转移位和至少一个清洗分离转移位,转移单元可在孵育转移位和清洗分离位之间转移反应容器,不仅可以实现灵活的孵育,而且还可以解决现有技术中必须采用多个清洗分离机构实现两步法测试的问题,充分实现高效的清洗分离。此外,测量装置可根据整机布局或结构实现的需要,灵活安排或布置,比如可直接安装在反应单元上、设置在独立位置上或安装在独立测量盘上等,解决了现有技术中测量装置布置受限、测量环境易干扰等问题。本发明提高了分析装置的工作效率和降低了自动化功能的实现难度,很好解决
了目前自动化仪器体积大、检测速度慢、成本高、性能差等技术难题,不但节约了实验室空间,提高了测试效率,而且有利于减少费用开支,减轻受测者负担,最终节约了大量的自然资源和社会资源。The invention realizes the incubation and washing separation of the reactants in the reaction vessel centering on the reaction unit, and at least one incubation transfer position and at least one cleaning separation transfer position are arranged on the reaction unit, and the transfer unit can be transferred between the incubation transfer position and the cleaning separation position. The reaction vessel can not only realize flexible incubation, but also solve the problem that the two-step test must be implemented by using multiple cleaning and separating mechanisms in the prior art, and fully realize efficient cleaning separation. In addition, the measuring device can be flexibly arranged or arranged according to the needs of the whole machine layout or structure realization, for example, can be directly installed on the reaction unit, set in a separate position or mounted on an independent measuring disk, etc., and solves the measurement in the prior art. The device layout is limited, and the measurement environment is easy to interfere. The invention improves the working efficiency of the analysis device and reduces the difficulty of realizing the realization of the automation function, and is well solved
At present, the technical problems of large size, slow detection speed, high cost and poor performance of the automatic instrument not only save the laboratory space, improve the test efficiency, but also help reduce the expenses, reduce the burden on the testee, and ultimately save a lot of Natural and social resources.
图1是一步法反应模式示意图;Figure 1 is a schematic diagram of a one-step reaction mode;
图2是一步法反应模式(另一种信号测量方式)示意图;Figure 2 is a schematic diagram of a one-step reaction mode (another signal measurement mode);
图3是延时一步法和两步法反应模式示意图;Figure 3 is a schematic diagram of a one-step and two-step reaction mode;
图4是本发明自动分析装置的第一种实施方式示意图;Figure 4 is a schematic view showing a first embodiment of the automatic analyzer of the present invention;
图5是一步法测试流程图;Figure 5 is a one-step test flow chart;
图6是延时一步法测试流程图;Figure 6 is a flow chart of a one-step delay test;
图7是两步法测试流程图;Figure 7 is a two-step test flow chart;
图8是本发明自动分析装置的第二种实施方式示意图;Figure 8 is a schematic view showing a second embodiment of the automatic analyzing device of the present invention;
图9是本发明自动分析装置的第三种实施方式示意图;Figure 9 is a schematic view showing a third embodiment of the automatic analyzer of the present invention;
图10是本发明自动分析装置的第四种实施方式示意图;Figure 10 is a schematic view showing a fourth embodiment of the automatic analyzer of the present invention;
图11是本发明自动分析装置的第五种实施方式示意图。Figure 11 is a schematic view showing a fifth embodiment of the automatic analyzer of the present invention.
下面通过具体实施方式结合附图对本发明作进一步详细说明。The present invention will be further described in detail below with reference to the accompanying drawings.
本发明的一种自动分析装置,包括:加注单元,加注样本和/或试剂到反应容器,反应单元,孵育并清洗分离反应容器内的反应物,测量装置,测量反应容器内的反应信号,转移单元,在不同位置之间转移反应容器,所述反应单元包括一个旋转装置,所述旋转装置上设置若干个反应容器位,用于承载和固定反应容器,所述反应单元上设置至少一个孵育转移位和至少一个清洗分离转移位,所述反应单元的至少一个孵育转移位和至少一个清洗分离转移位在转移单元的水平运动范围内。An automatic analysis device of the present invention comprises: a filling unit, filling a sample and/or a reagent into a reaction vessel, a reaction unit, incubating and washing the reactants in the separation reaction vessel, measuring the device, and measuring the reaction signal in the reaction vessel a transfer unit that transfers the reaction vessel between different positions, the reaction unit comprising a rotating device, wherein the rotating device is provided with a plurality of reaction vessel positions for carrying and fixing the reaction vessel, and at least one of the reaction units is disposed Incubating the transfer site and at least one wash separation transfer site, at least one incubation transfer site and at least one wash separation transfer site of the reaction unit are within a range of horizontal motion of the transfer unit.
反应容器为样本和试剂的反应提供反应场所,可以是各种形状和构造的反应管、反应杯、多个腔的反应杯条、反应芯片等,一般为一次性使用。反应容器的材料通常为塑料,如聚苯乙烯等。反应容器可以在内壁预先包被抗原或抗体,也可不包被,也可在其内预先存放包被好的磁珠或塑料球。反应容器的存储和供给由反应容器供给单元完成。为了精简机构,反应容器供给单元优选采用预排列式,反应容器预先排列在反应容器托盘、盒或反应容器架、槽道上,反应容器供给单元每次可将整盘、整盒反应容器或一排、一列反应容器输送至目标位置。其他实施例中反应容器供给单元可以是料仓式,反应容器可以成包散乱倒入反应容器供给单元的料仓中,然后反应容器供给单元自动将反应容器逐次单个排序,供给反应容器到转移单元。The reaction vessel provides a reaction site for the reaction of the sample and the reagent, and may be a reaction tube of various shapes and configurations, a reaction cup, a reaction cup of a plurality of chambers, a reaction chip, etc., and is generally used at one time. The material of the reaction vessel is usually a plastic such as polystyrene. The reaction container may be coated with an antigen or an antibody in advance on the inner wall, or may be pre-stored with a magnetic bead or a plastic ball. The storage and supply of the reaction vessel is completed by the reaction vessel supply unit. In order to streamline the mechanism, the reaction vessel supply unit is preferably pre-arranged, and the reaction vessel is pre-arranged in the reaction vessel tray, the box or the reaction vessel rack, and the channel, and the reaction vessel supply unit can be used for the whole tray, the whole box of reaction vessels or a row at a time. A series of reaction vessels are delivered to the target location. In other embodiments, the reaction vessel supply unit may be a silo type, and the reaction vessel may be poured into a silo of the reaction vessel supply unit in a scattered manner, and then the reaction vessel supply unit automatically sorts the reaction vessels one by one, and supplies the reaction vessel to the transfer unit. .
反应容器在本发明装置中不同位置之间的转移可由转移单元完成。转移单元可以是任何合适的可以转移或移动反应容器的机构,本发明优选的转移单元主要包括驱动机构、水平运动机械臂、抓放机构等结构。抓放机构通常为机械手指,可以抓放反应容器,水平运动机械臂在驱动机构驱动下可沿着X向、Y向、X向和Y向、径向、周向、径向和周向等方向移动抓放机构,将抓放机构抓取的反应容器移动到不同位置。除了水平运动外,转移单元还可做上下运动,将反应容器放入不同的位置或从不同的位置取出。根据测试速度和整机布局不同,可设置一个或多个转移单元。The transfer of the reaction vessel between different locations in the apparatus of the invention can be accomplished by the transfer unit. The transfer unit can be any suitable mechanism for transferring or moving the reaction vessel. The preferred transfer unit of the present invention primarily comprises a drive mechanism, a horizontal motion robot arm, a pick and place mechanism, and the like. The pick and place mechanism is usually a mechanical finger, which can hold the reaction container. The horizontal motion mechanical arm can be driven along the X direction, the Y direction, the X direction and the Y direction, the radial direction, the circumferential direction, the radial direction and the circumferential direction by the driving mechanism. Move the pick and place mechanism in the direction, and move the reaction container grabbed by the pick and place mechanism to different positions. In addition to horizontal movement, the transfer unit can also move up and down, placing the reaction vessels in different positions or taking them out from different locations. Depending on the test speed and the overall layout, one or more transfer units can be set.
加注单元完成样本、试剂的加注。加注单元一般由钢针或一次性吸嘴(Tip)、加注运动驱动机构、注射器或注液泵、阀、流体管路以及清洗池(当采用Tip时也可没有清洗池)等组件构成。为了完成吸取样本、试剂及其加注动作,加注单元除了可以上下运动外,还可以水平运动,水平运动通常有旋转、X向、Y向等几种运动形式及其组合。加注单元可以是一个,既加样本又加试剂,这样可使整机结构更紧凑和成本更低。为了提高测试速度,
加注单元还可进一步包括一个或几个样本加注单元、一个或几个试剂加注单元,样本加注单元只加注样本或加注样本和部分试剂,试剂加注单元加注试剂。The filling unit completes the filling of the sample and the reagent. The filling unit is generally composed of a steel needle or a disposable nozzle (Tip), a motion driving mechanism, a syringe or a liquid injection pump, a valve, a fluid line, and a cleaning tank (or a cleaning pool when a Tip is used). . In order to complete the suction sample, the reagent and the filling action, the filling unit can move horizontally in addition to the up and down movement, and the horizontal movement usually has several movement forms such as rotation, X direction and Y direction, and a combination thereof. The filling unit can be one, adding both the sample and the reagent, which makes the structure of the whole machine more compact and lower cost. In order to improve the test speed,
The filling unit may further comprise one or several sample filling units, one or several reagent filling units, the sample filling unit only filling the sample or filling the sample and a part of the reagent, and the reagent filling unit is filling the reagent.
为了方便加注单元的加注,本发明还可包括加注站。加注站位于转移单元和加注单元的运动范围内或可通过水平运动运动到转移单元和加注单元的运动范围内。加注站接收和承载转移单元转移过来的反应容器、接受加注单元向反应容器内加注样本和试剂。加注站上设置反应容器位,用于放置需要加注样本和试剂的反应容器。为了使样本和试剂混合更均匀、反应更充分,同时为了精简整机结构和缩小体积,本发明优选在加注站集成混匀机构,对每次加注后的反应容器进行超声混匀、偏向旋转或震荡混匀。当然,也可以将混匀机构,比如超声波发生器集成于加注单元,在加注样本和试剂的同时或加注动作完成后由加注单元产生的超声波实现混匀。本领域内技术人员可以理解,加注站也可不集成混匀机构,混匀还可由加注单元的吸排动作或冲击力完成。In order to facilitate the filling of the filling unit, the invention may also include a filling station. The filling station is located within the range of motion of the transfer unit and the filling unit or can be moved by horizontal motion to the range of motion of the transfer unit and the filling unit. The filling station receives and carries the reaction vessel transferred from the transfer unit, and accepts the filling unit to fill the reaction container with the sample and the reagent. A reaction vessel is placed on the filling station for placing a reaction vessel to which the sample and reagents need to be filled. In order to make the sample and the reagent mixture more uniform and more complete, and in order to simplify the structure of the whole machine and reduce the volume, the present invention preferably integrates the mixing mechanism at the filling station, and ultrasonically mixes and biases the reaction container after each filling. Rotate or shake to mix. Of course, it is also possible to integrate a mixing mechanism, such as an ultrasonic generator, into the filling unit, and mix the ultrasonic waves generated by the filling unit at the same time as the filling of the sample and the reagent or after the filling operation is completed. It will be understood by those skilled in the art that the filling station may not integrate the mixing mechanism, and the mixing may be performed by the suction and discharge action or the impact force of the filling unit.
反应单元承载和固定反应容器。反应单元主要包括保温装置、旋转装置和清洗分离装置。保温装置外围通常具有保温棉等隔热材料,通常包裹或包围旋转装置的底部、周边和上部,侧面或底部内侧可设有加热装置和传感器,上部一般为盖板等结构,为反应单元提供恒温孵育环境并防止或减少反应单元热量的散失。当然,为了传热效率更高,加热装置也可以安装在旋转装置上。除了提供孵育环境外,保温装置还可支撑和固定清洗分离装置的磁场产生装置,为清洗分离提供磁场环境。此外,如果测量装置安装在反应单元上,保温装置不仅可以为测光装置提供安装位置,还可实现测光装置所需的暗室环境。旋转装置最好为一个,包括驱动、传动机构及相关的控制电路等,控制和带动旋转装置每隔固定时间(比如一个循环或周期)旋转固定的角度,转送所述反应容器位前进一定的位置(比如前进一个反应容器位)。旋转装置上设置若干个独立的孔、槽、托架、底座或其他适合承载反应容器的结构,定义为反应容器位。反应容器位除了承载反应容器位外,还可以固定反应容器。此处“固定”指反应容器在反应容器位内不会移动或滑动,但可随着反应容器位一起整体运动。这样可使反应容器与反应容器位贴合更紧,间隙更小,不仅有利于反应容器的传热孵育和精准定位,还可使旋转装置结构更精简、容纳更多反应容器位、生产制造成本更低,从而有效解决了某些现有技术中由于反应容器在反应容器位内移动而引起的传热效率差、空间浪费、结构复杂等缺点和缺陷。除了承载和固定外,反应单元还孵育反应容器内的反应物。对于需要信号孵育的测试,本发明的反应单元还可实现信号孵育功能。The reaction unit carries and fixes the reaction vessel. The reaction unit mainly includes a heat preservation device, a rotation device, and a cleaning separation device. The periphery of the heat preservation device usually has insulation materials such as heat insulating cotton, and usually wraps or surrounds the bottom, the periphery and the upper portion of the rotating device, and the heating device and the sensor may be disposed on the inner side of the side or the bottom, and the upper portion is generally a structure such as a cover plate to provide a constant temperature for the reaction unit. Incubate the environment and prevent or reduce the loss of heat in the reaction unit. Of course, for higher heat transfer efficiency, the heating device can also be mounted on the rotating device. In addition to providing an incubation environment, the holding device can also support and secure the magnetic field generating device of the cleaning separation device to provide a magnetic field environment for cleaning separation. In addition, if the measuring device is mounted on the reaction unit, the heat insulating device can not only provide a mounting position for the photometric device, but also a darkroom environment required for the photometric device. Preferably, the rotating device comprises a driving device, a transmission mechanism and an associated control circuit, etc., and controls and drives the rotating device to rotate at a fixed angle every fixed time (such as a cycle or a cycle), and forwards the reaction vessel to a certain position. (such as advancing a reaction container bit). The rotating device is provided with a plurality of independent holes, slots, brackets, bases or other structures suitable for carrying the reaction vessel, defined as the reaction vessel position. In addition to carrying the reaction vessel position, the reaction vessel can also hold the reaction vessel. By "fixed" herein is meant that the reaction vessel does not move or slide within the reaction vessel position, but can move integrally with the reaction vessel site. In this way, the reaction vessel and the reaction vessel can be tightly packed, and the gap is smaller, which not only facilitates heat transfer incubation and precise positioning of the reaction vessel, but also makes the structure of the rotating device more compact, accommodates more reaction vessel positions, and has manufacturing costs. Lower, which effectively solves some shortcomings and defects in the prior art due to poor heat transfer efficiency, space waste, and complicated structure due to the movement of the reaction vessel in the reaction vessel position. In addition to carrying and immobilizing, the reaction unit also incubates the reactants in the reaction vessel. For tests that require signal incubation, the reaction unit of the present invention can also implement a signal incubation function.
为了让反应容器进出反应单元,在反应单元上设置至少两个转移位。转移位定义为在转移单元水平运动范围内的、反应单元上反应容器进出反应单元的固定位置,其不随反应容器位的转动而转动。不同位置的反应容器位可在旋转装置的旋转下转送和定位到转移位,接收反应容器或退出其上的反应容器以使反应容器完成后续相应的功能,比如进入反应单元孵育或转移到其它反应容器位进行清洗分离等。根据反应容器进出的阶段和实现的主要功能不同,可将转移位分为孵育转移位和清洗分离转移位。本发明将需要孵育的反应容器进入反应单元、孵育一定时间后或孵育结束的反应容器转移出反应单元所通过的转移位定义为孵育转移位;将孵育一定时间后或孵育结束后需要清洗分离的反应容器进入反应单元或/和完成清洗分离的反应容器转移出反应单元所通过的转移位定义为清洗分离转移位。本发明反应单元的孵育转移位设置至少一个,特定的孵育转移位可根据需要只进不出、只出不进或既出又进反应容器,这样可以实现灵活的孵育时间和多样的整机布局。需要指出的是,为了解决现有技术的缺点和问题,通过孵育转移位进出反应单元的反应容器包括需要孵育一次、孵育两次或更多次的反应容器,这样可以将需要孵育一次、两次以及更多次的反应容器先集中孵育,从而减少反应单元的尺寸和提高反应单元的空间使用率。清洗分离转移位可设置一个,反应容器既出又进,这样可以使清洗分离装置更紧凑,也可设置至少一个,这样可以更灵活布置清洗分离装置。总之,反应单元上转移位的设置,解决了现
有技术中转移位分散在多个不同单元上以及需要孵育的反应容器分散放置的问题,不仅可使转移单元运动路径更少、距离更短,而且充分利用了反应单元的空间,从而使整机控制更简单、尺寸更小。In order to allow the reaction vessel to enter and exit the reaction unit, at least two transfer sites are provided on the reaction unit. The transfer position is defined as the fixed position of the reaction vessel in and out of the reaction unit on the reaction unit within the horizontal motion range of the transfer unit, which does not rotate with the rotation of the reaction vessel position. The reaction vessel position at different positions can be transferred and positioned to the transfer position under the rotation of the rotating device, and the reaction vessel can be received or withdrawn from the reaction vessel to complete the subsequent corresponding functions, such as entering the reaction unit for incubation or transferring to other reactions. The container position is cleaned and separated. Depending on the stage in which the reaction vessel enters and exits and the main functions achieved, the transfer position can be divided into an incubation transfer position and a wash separation transfer position. The present invention defines a transfer site through which the reaction vessel to be incubated into the reaction unit, after a certain period of incubation or at the end of the incubation, and the transfer site through which the reaction unit is transferred is defined as the incubation transfer position; after a certain period of incubation or after the end of the incubation, the separation needs to be cleaned. The transfer site through which the reaction vessel enters the reaction unit or/and the reaction vessel that completes the purge separation is transferred to the reaction unit is defined as the wash separation transfer site. The incubation unit of the present invention has at least one incubation transfer position, and the specific incubation transfer position can be inaccessible, only in or out, and into the reaction container as needed, so that flexible incubation time and various machine layouts can be realized. It should be noted that in order to solve the disadvantages and problems of the prior art, the reaction vessel that enters and exits the reaction unit by incubating the transfer site includes a reaction vessel that needs to be incubated once, incubated two or more times, so that it may be incubated once or twice. And more times the reaction vessel is concentrated in the first step, thereby reducing the size of the reaction unit and increasing the space utilization rate of the reaction unit. The cleaning separation transfer position can be set one by one, and the reaction container can be both retracted and further, so that the cleaning separation device can be made more compact, and at least one can be set, so that the cleaning separation device can be arranged more flexibly. In short, the setting of the transfer bit on the reaction unit solves the present
In the prior art, the problem that the transfer sites are dispersed on a plurality of different units and the reaction vessels that need to be incubated are dispersed can not only make the transfer unit have fewer moving paths, shorter distances, but also make full use of the space of the reaction unit, thereby making the whole machine Control is simpler and smaller.
反应单元除了上述的功能外,其上的清洗分离装置还可实现清洗分离,以去除反应物中未结合的成分。本发明反应单元的清洗分离装置包括磁场产生装置和冲洗机构。磁场产生装置提供磁场环境,使反应容器内的顺磁颗粒吸附到反应容器内壁。由于在磁场中的响应时间、移动距离和阻力等因素,顺磁性颗粒吸附到反应容器内壁需要一定的时间,通常为几秒到几十秒不等,这样在每次吸取废液(包括未结合成分)前,反应容器需要经过磁场一段时间。本发明的一种优选实施例中,磁场产生装置可直接安装或固定在反应单元的保温装置上,这样不仅可以节省额外的固定机构,降低成本,还可使磁铁产生装置更靠近反应容器位,从而减少顺磁颗粒的吸附时间,提高清洗分离效率。冲洗机构包括吸液和注液装置,抽吸反应容器内的未结合成分和向抽吸后的反应中注入清洗缓冲液。吸液装置包括吸液针、吸液管或吸液嘴等适合抽吸液体的吸液部,吸液部布置在反应单元的上方,可以通过驱动机构的带动进出反应容器位上的反应容器,抽吸反应容器内的未结合成分。注液装置包括注液针、管、嘴等适合排注液体的注液部,注液部同样布置在反应单元的反应容器位的上方,向抽吸后的反应容器内注入清洗缓冲液。每次冲洗包括一次吸液和一次注入清洗缓冲液和过程,一般冲洗三次或四次,即进行三次或四次冲洗,当然冲洗次数也可灵活多变。为了使清洗更彻底,残留更少,还可在注液位设置混匀器混匀反应容器或利用注液时的冲击力,在注清洗缓冲液同时或注清洗缓冲液后使顺磁性颗粒重悬浮和均匀分散在清洗缓冲液中。反应单元旋转装置转送反应容器到清洗分离装置时,清洗分离装置开始对反应容器进行清洗分离。此外,为了精简机构,清洗分离装置还可进一步耦合信号试剂加注机构,在反应容器完成清洗分离后,向其内加注全部或部分信号试剂,比如加注全部的第一、第二信号试剂等或只加注第一信号试剂等,余下的信号试剂可在测量时加注。这样可以充分利用清洗分离机构的功能,缩减了机构体积和节省了成本。In addition to the functions described above, the reaction unit can also perform cleaning separation to remove unbound components of the reactants. The cleaning and separating device of the reaction unit of the present invention comprises a magnetic field generating device and a flushing mechanism. The magnetic field generating device provides a magnetic field environment for adsorbing paramagnetic particles in the reaction vessel to the inner wall of the reaction vessel. Due to factors such as response time, moving distance and resistance in the magnetic field, it takes a certain time for the paramagnetic particles to adsorb to the inner wall of the reaction vessel, usually ranging from several seconds to several tens of seconds, so that each time the waste liquid is taken (including unbound Before the component), the reaction vessel needs to pass through the magnetic field for a period of time. In a preferred embodiment of the present invention, the magnetic field generating device can be directly mounted or fixed on the heat insulating device of the reaction unit, which not only saves an additional fixing mechanism, reduces the cost, but also brings the magnet generating device closer to the reaction container. Thereby reducing the adsorption time of the paramagnetic particles and improving the cleaning separation efficiency. The rinsing mechanism includes a liquid absorbing and injecting device that sucks unbound components in the reaction vessel and injects a washing buffer into the reaction after suction. The liquid absorption device comprises a liquid absorption part suitable for pumping liquid, such as a liquid suction needle, a liquid suction tube or a liquid suction nozzle, and the liquid absorption part is arranged above the reaction unit, and the reaction container can be driven into and out of the reaction container through the driving mechanism. The unbound components in the reaction vessel are aspirated. The liquid injection device includes a liquid injection portion suitable for discharging liquid, such as a liquid injection needle, a tube, a mouth, and the like, and the liquid injection portion is also disposed above the reaction container position of the reaction unit, and the cleaning buffer is injected into the reaction container after the suction. Each flush includes one aspirate and one injection buffer and process, usually three or four times, ie three or four rinses, although the number of flushes can be varied. In order to make the cleaning more thorough and less residue, it is also possible to set the mixer to mix the reaction vessel in the filling position or to use the impact force when injecting the liquid, and to make the paramagnetic particles heavy after the injection buffer or the cleaning buffer. Suspended and uniformly dispersed in the wash buffer. When the reaction unit rotating device transfers the reaction vessel to the washing and separating device, the washing and separating device starts cleaning and separating the reaction vessel. In addition, in order to streamline the mechanism, the cleaning and separating device may further couple the signal reagent filling mechanism, and after the cleaning and separation of the reaction container, all or part of the signal reagent is added thereto, for example, all the first and second signal reagents are added. Wait for or only add the first signal reagent, etc., and the remaining signal reagents can be added during the measurement. This makes full use of the function of the cleaning and separating mechanism, reducing the size of the mechanism and saving costs.
由以上描述可知,清洗分离装置布置在反应单元旋转装置周边或旋转装置上方,可以直接对反应单元旋转装置上的反应容器进行清洗分离,这样可以避免设置独立的清洗分离旋转装置,如独立的清洗分离盘或清洗分离轨道等,不仅精简了组件和整机机构,使整机机构更紧凑和成本更低,还避免了反应容器在独立的清洗分离装置和反应单元之间的转移,使整机控制流程更简单高效,从而提高处理效率和可靠性。It can be seen from the above description that the cleaning and separating device is arranged around the rotating unit of the reaction unit or above the rotating device, and the reaction container on the rotating unit of the reaction unit can be directly cleaned and separated, thereby avoiding the installation of independent cleaning and separating rotating device, such as independent cleaning. Separating the disc or cleaning the separation rail, etc., not only streamlining the components and the whole mechanism, making the whole mechanism more compact and lower in cost, but also avoiding the transfer of the reaction vessel between the independent cleaning and separating device and the reaction unit, so that the whole machine Control processes are simpler and more efficient, increasing processing efficiency and reliability.
测量装置对反应容器内的信号进行测量。信号为反应容器内加入信号试剂后产生的电信号、荧光信号或微弱化学发光信号等。测量装置包括微弱光探测器光电倍增管(PMT)或其他灵敏的光电感应器件,可把测量的光信号转换为电信号,传送至控制中心。此外,为了提高测量效率和保证测量一致性,测量装置还可进一步包括光信号收集和校准等光学装置。以微弱化学发光信号为例,为了避免环境光的干扰,本发明中测量装置对反应容器内的信号测量有三种实现方式。第一种实现方式中测量装置安装于反应单元,对反应单元反应容器位上的反应容器内的反应信号进行测量。这样可以充分利用反应单元上的反应容器位,使整机更为紧凑、成本更低。第二种实施方式中包括测量暗室和测量位,测量装置安装于测量暗室上,对测量位上的的反应容器内的信号进行测量。测量位在转移单元的水平运动范围内或可水平运动到所述转移单元的水平运动范围内。第三种实施方式主要包括测量盘、测量暗室和测量装置等。测量盘包括以所述测量盘旋转中心为圆心的至少一圈反应容器位,用于承载需要进行测量的反应容器。对于需要信号孵育的测试来说,测量盘上的反应容器位还可实现信号孵育功能。通过测量盘的旋转,可把其上的任一反应容器位上的反应容器旋转到测量装置进行测量,从而实现灵活的信号孵育,提高测试的灵活性和效率。测量单元的测量暗室包裹或包围在测量盘的周边,为测量单元提供密闭的暗室环境。
进一步地,为了实现有些测试的信号孵育功能,测量暗室的侧面或底部还可设置加热装置和传感器,为测量单元提供恒温孵育环境并防止或减少反应单元热量的散失。当然,为了传热效率更高,加热装置也可以安装在测量盘上。测量装置可以通过通用方式连接或安装到测量暗室上,比如直接安装固定在测量暗室上或通过光纤连接安装到测量暗室上,这样可以直接对测量盘反应容器位上的反应容器内的信号进行测量,可使处理效率和可靠性更高。本发明的测量装置可以根据设计需要灵活布局,不仅容易实现暗室环境,还可实现灵活的信号孵育,解决了现有技术暗室结构复杂、测量装置布局困难等缺点。The measuring device measures the signal in the reaction vessel. The signal is an electrical signal, a fluorescent signal or a weak chemiluminescence signal generated after the signal reagent is added to the reaction vessel. The measuring device includes a weak photodetector photomultiplier tube (PMT) or other sensitive photoelectric sensing device that converts the measured optical signal into an electrical signal for transmission to the control center. In addition, in order to improve measurement efficiency and ensure measurement uniformity, the measuring device may further include optical devices such as optical signal collection and calibration. Taking the weak chemiluminescence signal as an example, in order to avoid interference of ambient light, the measuring device of the present invention has three implementations for measuring the signal in the reaction container. In the first implementation, the measuring device is installed in the reaction unit, and the reaction signal in the reaction vessel at the reaction vessel reaction vessel position is measured. This makes full use of the reaction vessel position on the reaction unit, making the machine more compact and less costly. The second embodiment includes measuring the darkroom and the measuring position, and the measuring device is mounted on the measuring darkroom to measure the signal in the reaction vessel at the measuring position. The measurement position is within the horizontal range of motion of the transfer unit or can be moved horizontally to the horizontal range of motion of the transfer unit. The third embodiment mainly includes a measuring disk, a measuring dark room, a measuring device, and the like. The measuring disk comprises at least one reaction vessel position centered on the center of rotation of the measuring disk for carrying a reaction vessel to be measured. For tests that require signal incubation, the reaction vessel position on the measurement pan also enables signal incubation. By measuring the rotation of the disc, the reaction vessel on any of the reaction vessel positions can be rotated to the measuring device for measurement, thereby achieving flexible signal incubation and improving test flexibility and efficiency. The measuring darkroom of the measuring unit is wrapped or enclosed around the measuring disc to provide a closed darkroom environment for the measuring unit.
Further, in order to realize the signal incubation function of some tests, the heating device and the sensor may be disposed at the side or the bottom of the measurement darkroom to provide a constant temperature incubation environment for the measurement unit and prevent or reduce the loss of heat of the reaction unit. Of course, for higher heat transfer efficiency, the heating device can also be mounted on the measuring plate. The measuring device can be connected or mounted to the measuring darkroom in a general manner, such as directly mounted on the measuring darkroom or mounted to the measuring darkroom via a fiber optic connection, so that the signal in the reaction vessel on the measuring disc reaction vessel can be directly measured. Can make processing efficiency and reliability higher. The measuring device of the invention can be flexibly arranged according to the design requirements, not only easy to realize the darkroom environment, but also realize flexible signal incubation, and solves the defects of the complicated structure of the prior art darkroom and the difficulty of the layout of the measuring device.
此外,为了输送样本、存储试剂,本发明的自动分析装置还可设置样本输送单元、试剂存储单元等单元。Further, in order to transport the sample and store the reagent, the automatic analyzer of the present invention may further be provided with a unit such as a sample transport unit, a reagent storage unit, and the like.
样本输送单元用于放置待检样本管并将目标样本管输送至吸样本位。样本输送单元有轨道进样、样本盘进样和固定区域进样三种主要方式,样本管通常放置在样本架上,每个样本架一般放置5个或10个样本管,样本架放置于传输轨道上、样本盘上或分析装置的固定区域。The sample transport unit is used to place the sample tube to be inspected and deliver the target sample tube to the sample site. The sample transport unit has three main modes: orbital injection, sample tray injection and fixed area injection. The sample tubes are usually placed on the sample holder. Each sample holder is usually placed with 5 or 10 sample tubes, and the sample holder is placed in the transmission. A fixed area on the track, on the sample tray, or on the analysis device.
试剂存储单元冷藏试剂并将目标试剂转送至吸试剂位。试剂存储单元通常采用试剂盘和固定试剂存储区两种方式,为了保证试剂的稳定性,试剂盘一般具有制冷功能,如4-10℃。试剂盘上一般设置若干个试剂容器位,用于放置试剂容器。每个试剂容器设置若干个独立的腔体,用于存放不同的试剂组分,如磁微粒试剂、酶标试剂、稀释液等试剂组分。The reagent storage unit refrigerates the reagent and transfers the target reagent to the aspirating reagent position. The reagent storage unit usually adopts two ways of reagent tray and fixed reagent storage area. In order to ensure the stability of the reagent, the reagent tray generally has a cooling function, such as 4 - 10 °C. A plurality of reagent container positions are generally set on the reagent tray for placing the reagent container. Each reagent container is provided with a plurality of independent chambers for storing different reagent components, such as magnetic particle reagents, enzyme labeling reagents, diluents and the like.
本发明自动分析装置的第一种实施方式,参考图4。自动分析装置100主要包括样本输送单元30、试剂存储单元40、加注单元20、加注站90、反应容器供给单元70、转移单元50、反应单元10以及测量装置86等。下面分别叙述各部分的功能和作用。A first embodiment of the automatic analyzer of the present invention is referred to FIG. The automatic analysis device 100 mainly includes a sample delivery unit 30, a reagent storage unit 40, a filling unit 20, a filling station 90, a reaction container supply unit 70, a transfer unit 50, a reaction unit 10, a measuring device 86, and the like. The functions and functions of each part are described below.
样本输送单元30用于放置待检样本管31并将目标样本管输送至吸样本位。本实施例中,样本输送单元30为样本盘,样本盘上放置弧形样本架(图中未标出)上,每个弧形样本架放置10个样本管31。样本盘可在控制中心的控制下由驱动机构带动将目标样本转送至吸样本位,吸样本位位于加注单元20的水平动范围与样本管中心圆的交点处。The sample delivery unit 30 is used to place the sample tube 31 to be inspected and deliver the target sample tube to the sample site. In this embodiment, the sample transport unit 30 is a sample tray on which a curved sample holder (not shown) is placed, and each of the curved sample holders is placed with 10 sample tubes 31. The sample tray can be driven by the driving mechanism to transfer the target sample to the suction sample position under the control of the control center, and the suction sample position is located at the intersection of the horizontal movement range of the filling unit 20 and the center circle of the sample tube.
试剂存储单元40冷藏试剂容器41并将目标试剂转送至吸试剂位。本实施例中,试剂存储单元40为试剂盘,设置25个试剂位,可容纳25个试剂容器41(或试剂盒、试剂瓶,为表述方便,以下简称试剂瓶)。本实施例中,每个试剂瓶41设置4个腔体41a、41b、41c、41d,可用于存放磁微粒试剂、酶标试剂、稀释液等试剂组分。试剂盘可在控制中心的控制下由驱动机构带动将目标试剂瓶转送至吸试剂位,吸试剂位位于加注单元水平运动范围与试剂腔中心圆的交点处,本实施例中,与对应4个试剂组分对应,有4个吸试剂位(图中未标出)。The reagent storage unit 40 refrigerates the reagent container 41 and transfers the target reagent to the aspirating reagent position. In the present embodiment, the reagent storage unit 40 is a reagent tray, and 25 reagent positions are provided, and 25 reagent containers 41 (or kits and reagent bottles are conveniently used for the description, hereinafter referred to as reagent bottles). In this embodiment, each of the reagent bottles 41 is provided with four chambers 41a, 41b, 41c, and 41d, and can be used for storing reagent components such as magnetic particle reagents, enzyme labeling reagents, and diluents. The reagent tray can be driven by the driving mechanism to transfer the target reagent bottle to the suction reagent position under the control of the control center. The suction reagent position is located at the intersection of the horizontal movement range of the filling unit and the center circle of the reagent chamber. In this embodiment, the corresponding 4 Corresponding to the reagent components, there are 4 aspirating reagent sites (not shown).
加注单元20完成样本、试剂的加注。加注单元水平运动范围与样本盘30上的样本位、试剂盘40上的试剂位、测量盘上的反应容器位分别相交,交点处分别为吸样本位、吸试剂位和加注位。本实施例中,加注单元为单一加样机构,可做上下和水平旋转运动,既加注样本又加注试剂,这样可使整机结构更紧凑和成本更低。在一些实施例中,加注单元20上还可集成超声波发生器等混匀机构,对每次加注后的反应容器进行超声混匀。The filling unit 20 completes the filling of the sample and the reagent. The horizontal movement range of the filling unit intersects the sample position on the sample tray 30, the reagent position on the reagent disk 40, and the reaction container position on the measuring disk, respectively, and the intersection point is the suction sample position, the suction reagent position, and the filling position. In this embodiment, the filling unit is a single sample loading mechanism, which can perform up and down and horizontal rotation movements, and both the sample and the reagent are added, so that the structure of the whole machine is more compact and the cost is lower. In some embodiments, a mixing mechanism such as an ultrasonic generator may be integrated on the filling unit 20 to ultrasonically mix the reaction container after each filling.
加注站90位于转移单元50和加注单元20的水平运动轨迹下,接收和承载转移单元50转移过来的反应容器、接受加注单元20向反应容器内加注样本和试剂。加注站上设置反应容器位,用于放置需要加样本和试剂的反应容器。本实施例中,在加注站集成混匀机构,对每次加注后的反应容器进行超声混匀或偏心震荡混匀,优选偏心震荡混匀,这样技术实现难度更低,结构更紧凑。The filling station 90 is located under the horizontal movement track of the transfer unit 50 and the filling unit 20, and receives and transfers the reaction container transferred from the transfer unit 50, and accepts the filling unit 20 to fill the reaction container with the sample and the reagent. A reaction vessel is placed on the filling station for placing a reaction vessel to which the sample and reagents need to be added. In this embodiment, the mixing mechanism is integrated in the filling station, and the reaction container after each filling is ultrasonically mixed or eccentrically oscillated and mixed, preferably eccentrically oscillating and mixing, so that the technology is less difficult to implement and the structure is more compact.
反应容器供给单元70存放和提供反应容器。本实施例中,为了使整机更为紧凑和成本更低,反应容器供给单元采用预先排列式。反应容器供给单元70包括两个反应容器托盘,反应容器托盘上设置若干数量的反应容器位,存放未使用的反应容器。反应容器供给
单元70在转移单元50的水平运动范围内,这样转移单元50可以遍历托盘上每个反应容器位上的未使用的反应容器,为新开始的测试提供未使用的反应容器。The reaction vessel supply unit 70 stores and supplies a reaction vessel. In this embodiment, in order to make the whole machine more compact and lower in cost, the reaction container supply unit adopts a pre-arranged type. The reaction vessel supply unit 70 includes two reaction vessel trays on which a number of reaction vessel positions are disposed to store unused reaction vessels. Reaction vessel supply
Unit 70 is within the horizontal range of motion of transfer unit 50 such that transfer unit 50 can traverse unused reaction vessels at each reaction vessel location on the tray to provide an unused reaction vessel for the newly initiated test.
转移单元50在自动分析装置100的不同位置之间转移反应容器。本实施中,转移单元50设置为1个,可做三维运动,这样可使整机更为紧凑和成本更低。转移单元50包括X向运动机械臂50b、Y向导轨50a、Y向运动机械臂50c以及垂直运动机构和机械手指(图中未标出)等机构。转移单元50可同时沿着X向、Y向水平移动机械手指,水平运动范围覆盖边界矩形56内的范围,可将反应容器在反应容器供给单元70、反应单元10上的9个孵育转移位(12a1-3、12b1-3,12c1-3)、反应单元10上的2个清洗分离转移位(12d1和12d2)、丢反应容器位60之间转移。此外,由于转移单元50运动范围覆盖反应单元10上的多个孵育转移位,转移单元可以通过不同的孵育转移位放入反应容器或从不同的孵育转移位转移出应容器来实现灵活的孵育时间。The transfer unit 50 transfers the reaction vessels between different positions of the automated analysis device 100. In the present embodiment, the transfer unit 50 is set to one, and the three-dimensional movement can be performed, which makes the whole machine more compact and lower in cost. The transfer unit 50 includes an X-direction moving robot arm 50b, a Y-direction guide rail 50a, a Y-direction moving robot arm 50c, and a vertical motion mechanism and a mechanical finger (not shown). The transfer unit 50 can simultaneously move the mechanical finger horizontally along the X direction and the Y direction, and the horizontal movement range covers the range within the boundary rectangle 56, and the 9 incubation transfer positions of the reaction container on the reaction container supply unit 70 and the reaction unit 10 can be 12a 1-3 , 12b 1-3 , 12c 1-3 ), 2 cleaning separation transfer sites (12d 1 and 12d 2 ) on the reaction unit 10, and transfer between the lost reaction vessel sites 60. In addition, since the range of motion of the transfer unit 50 covers a plurality of incubation transfer sites on the reaction unit 10, the transfer unit can be placed in the reaction vessel through different incubation transfer positions or transferred from the different incubation transfer sites to achieve a flexible incubation time. .
反应单元10承载和固定反应容器、孵育并清洗分离反应容器内的反应物。本实施例中,反应单元10的保温装置为锅体12和上盖(图中未标出),旋转装置为一个反应盘11、清洗分离装置为16。锅体12侧面或底部里侧有加热器和传感器,包围反应盘11的底部和周边,为反应单元10提供恒温孵育环境,防止或减少反应单元10热量的散失。除了提供孵育环境外,锅体12还支撑和固定清洗分离装置16的磁场产生装置,为清洗分离提供磁场环境。本实施例中,清洗分离装置16的磁铁产生装置为永磁体装置,这样可以提供更强和更稳定的磁场环境。清洗分离装置16的冲洗机构包括吸液装置和注液装置以及混匀机构。清洗分离装置16还可耦合信号试剂加注机构,在反应单元10反应容器位上的反应容器完成清洗分离后,向其内加注全部或部分信号试剂。The reaction unit 10 carries and fixes the reaction vessel, incubates and cleans the reactants in the separation reaction vessel. In the present embodiment, the heat retaining device of the reaction unit 10 is a pot body 12 and an upper cover (not shown), and the rotating device is a reaction disk 11 and a cleaning and separating device 16 . A heater and a sensor are arranged on the side or the bottom side of the pot body 12, surrounding the bottom and the periphery of the reaction disk 11, providing a constant temperature incubation environment for the reaction unit 10 to prevent or reduce the loss of heat of the reaction unit 10. In addition to providing an incubation environment, the pot 12 also supports and secures the magnetic field generating means of the cleaning separation device 16 to provide a magnetic field environment for cleaning separation. In the present embodiment, the magnet generating device of the cleaning and separating device 16 is a permanent magnet device, which can provide a stronger and more stable magnetic field environment. The rinsing mechanism of the cleaning separation device 16 includes a liquid absorbing device and a liquid injection device, and a mixing mechanism. The cleaning and separating device 16 can also be coupled with a signal reagent filling mechanism, and after the cleaning container is separated from the reaction vessel at the reaction vessel position of the reaction unit 10, all or part of the signal reagent is filled therein.
本实施例中的测量装置86直接安装在锅体12的侧面上,当然也可安装在保温装置的上盖上,可直接对反应盘11的反应容器位上的反应容器内的反应信号进行测量。这样可以充分利用反应单元上的反应容器位,使整机更为紧凑、成本更低。The measuring device 86 in this embodiment is directly mounted on the side of the pot body 12, and of course can also be mounted on the upper cover of the heat retaining device, and can directly measure the reaction signal in the reaction vessel on the reaction vessel position of the reaction disk 11. . This makes full use of the reaction vessel position on the reaction unit, making the machine more compact and less costly.
本实施例中,反应单元10的反应盘11可绕中心轴旋转,其上设置以旋转中心为圆心的四圈反应容器位,当然圈数是可以改变的,但至少为2圈,比如可以是2圈、3圈、5圈或更多圈等,每圈设置若干个反应容器位,每圈的反应容器位数量可以相同也可以不同,本实施例中,每圈设置30个反应容器位。每个反应容器位为反应盘上尺寸合适的孔槽,可以容纳一个反应容器,承载和固定反应容器,反应容器放进相应的反应容器位后,在反应容器位内不会发生移动或滑动。反应盘内三圈11a、11b、11c上的反应容器位实现孵育功能,容纳正在孵育的反应容器。外圈11d上的反应容器位主要容纳孵育结束或孵育一定时间后即将或正在清洗分离的反应容器,主要实现清洗分离和测量功能。为了反应容器可以进出反应盘11不同圈上的反应容器位和能够实现灵活的孵育时间,在保温装置的上盖上设置开孔,开孔所在位置即为转移位,共设置了9个孵育转移位13a1-3、13b1-3、13c1-3和2个清洗分离转移位13d1和13d2,其中,孵育转移位13a1-3、13b1-3、13c1-3分别对应反应盘内三圈11a、11b、11c,分别供反应容器进出11a、11b、11c上的反应容器位;清洗分离转移位12d1和12d2对应反应盘外圈11d,供反应容器进出11d上的反应容器位。反应盘每隔固定时间旋转固定的角度,可以逆时针旋转或顺时针旋转,比如每30秒旋转12度,前进一个反应容器位。通过反应盘旋转,反应容器位上的反应容器可被转送到孵育转移位或清洗分离转移位。反应盘每次旋转后的间歇时间内,转移单元可将反应容器从多个孵育转移位和清洗分离转移位移进、移出反应盘。反应容器通过孵育转移位进入反应盘后,开始在11a、11b或11c上的反应容器位孵育,孵育结束或孵育一定时间后再从孵育转移位转移出来。通过孵育转移位进出反应盘的反应容器包括孵育一次、孵育两次或更多次的反应容器,这样可以充分利用反应盘的空间。需要指出的是,反应容器可以在内三圈11a、11b、11c完成孵育,再转移到外圈11d上进行清洗分离,也可在内三圈完成一定时间的孵
育后,比如完成大部分时间的孵育,再转移到外圈11d,然后在反应盘转送到磁分离装置的过程中完成余下时间的孵育。前一种实现方式中内三圈无需设置很多反应容器位,就可支持完成反应容器的孵育,外圈也无需额外的反应容器位用于孵育,从而可以使反应盘尺寸更小,成本更低。对于后一种实现方式,举例如下,若一个测试的反应容器需要孵育25分钟,可以先在内三圈11a、11b、11c的一圈或几圈上完成大部分时间如24分钟的孵育,再转移到外圈11d上,在转送到清洗分离装置前完成余下1分钟的孵育。这种方案由于外圈分担了部分孵育的功能,可以适当减少内三圈的反应容器位数量,这样可以平衡内外圈反应容器位的数量,从而优化反应盘的尺寸并充分利用反应盘的内部空间。In this embodiment, the reaction disk 11 of the reaction unit 10 is rotatable about a central axis, and a four-turn reaction vessel position centered on the center of rotation is disposed thereon. Of course, the number of turns can be changed, but at least 2 turns, for example, 2 cycles, 3 turns, 5 turns or more, etc., a plurality of reaction vessel positions are arranged per turn, and the number of reaction vessel positions per turn may be the same or different. In this embodiment, 30 reaction vessel positions are set per turn. Each reaction vessel is located in a suitable size tank on the reaction tray, and can accommodate a reaction vessel for carrying and fixing the reaction vessel. After the reaction vessel is placed in the corresponding reaction vessel position, no movement or sliding occurs in the reaction vessel position. The reaction vessel position on the three turns 11a, 11b, 11c in the reaction tray achieves an incubation function to accommodate the reaction vessel being incubated. The reaction vessel position on the outer ring 11d mainly accommodates the separation of the reaction vessel after the end of the incubation or after a certain period of incubation, mainly to achieve the cleaning separation and measurement functions. In order to allow the reaction vessel to enter and exit the reaction vessel on different circles of the reaction disk 11 and to achieve flexible incubation time, an opening is provided on the upper cover of the thermal insulation device, and the position of the opening is the transfer position, and a total of 9 incubation transfers are set. Positions 13a 1-3 , 13b 1-3 , 13c 1-3 and 2 wash separation transfer sites 13d 1 and 13d 2 , wherein the incubation sites 13a 1-3 , 13b 1-3 , 13c 1-3 correspond to the reactions Three turns 11a, 11b, 11c in the tray are respectively supplied to the reaction vessel in the reaction vessel 11a, 11b, 11c; the cleaning separation transfer sites 12d 1 and 12d 2 correspond to the reaction disk outer ring 11d, and the reaction vessel enters and exits 11d. Container bit. The reaction plate is rotated at a fixed angle every fixed time and can be rotated counterclockwise or clockwise, for example by 12 degrees every 30 seconds, advancing a reaction vessel position. By rotating the reaction disk, the reaction vessel at the reaction vessel can be transferred to the incubation transfer site or to the wash separation site. The transfer unit can shift the reaction vessel from the plurality of incubation transfer positions and the cleaning separation into and out of the reaction tray during the intermittent time after each rotation of the reaction tray. After the reaction vessel enters the reaction disk by incubating the transfer site, it begins to incubate in the reaction vessel at 11a, 11b or 11c, and is transferred from the incubation transfer site after the incubation or incubation for a certain period of time. The reaction vessel that enters and exits the reaction disk by incubating the transfer site includes a reaction vessel that is incubated once, incubated twice or more, so that the space of the reaction disk can be fully utilized. It should be noted that the reaction vessel can be incubated on the inner three rings 11a, 11b, 11c, and then transferred to the outer ring 11d for washing and separation, or after a certain period of incubation in the inner three rings, for example, most of the time is completed. Incubate, transfer to the outer ring 11d, and then complete the incubation for the remainder of the time while the reaction disk is transferred to the magnetic separation device. In the former implementation, it is possible to support the completion of the incubation of the reaction vessel without the need for a plurality of reaction vessel positions in the inner three circles, and the outer ring does not require an additional reaction vessel position for the incubation, thereby making the reaction tray smaller in size and lower in cost. . For the latter implementation, for example, if a test reaction container needs to be incubated for 25 minutes, it can be done for most of the time, such as 24 minutes, on one or several turns of the inner three rings 11a, 11b, and 11c. Transfer to the outer ring 11d and complete the remaining 1 minute incubation before transferring to the wash separation unit. Because this kind of scheme shares the function of partial incubation, the number of reaction vessels in the inner three rings can be appropriately reduced, so that the number of reaction vessels in the inner and outer rings can be balanced, thereby optimizing the size of the reaction disk and making full use of the internal space of the reaction disk. .
下面以一个一步法测试为例,结合附图4和5,简述自动分析装置100的测量流程和步骤。测试开始后,Taking a one-step test as an example, the measurement flow and steps of the automatic analysis device 100 will be briefly described with reference to FIGS. 4 and 5. After the test starts,
步骤200加载反应容器:转移单元50从反应容器供给单元70转移一个未使用的反应容器到加注站90的反应容器位上。Step 200 loads the reaction vessel: Transfer unit 50 transfers an unused reaction vessel from reaction vessel supply unit 70 to the reaction vessel location of fill station 90.
步骤201加注样本和试剂:加注单元20分别从吸样本位和吸试剂位吸取样本和试剂加注到加注站90上的反应容器内。The step 201 is to fill the sample and the reagent: the filling unit 20 respectively sucks the sample and the reagent from the suction sample position and the suction reagent position into the reaction container on the filling station 90.
步骤202混匀:若需要混匀,则混匀机构对反应容器内的样本和试剂进行混匀。若不需要混匀,则省略该步骤。Step 202: Mixing: If mixing is required, the mixing mechanism mixes the sample and reagents in the reaction vessel. If no mixing is required, this step is omitted.
步骤203孵育:转移单元50从加注站90将加注完样本和试剂的反应容器通过孵育转移位(12a1-3、12b1-3、12c1-3中的一个)转移到反应盘11内三圈11a、11b、11c上的某个反应容器位,反应容器开始在反应盘上孵育。反应容器孵育的同时,每隔固定时间随反应盘11旋转前进1个位置。孵育时间因具体测试项目而异,一般为5-60分钟。Step 203: The transfer unit 50 transfers the reaction container filled with the sample and the reagent from the filling station 90 to the reaction tray 11 by incubating the transfer position (one of 12a 1-3 , 12b 1-3 , 12c 1-3 ) Within one of the three reaction chambers 11a, 11b, 11c, the reaction vessel begins to incubate on the reaction tray. While the reaction vessel is being incubated, the reaction disk 11 is rotated one position at a fixed time. The incubation time varies depending on the specific test item, typically 5 to 60 minutes.
步骤204清洗分离:孵育完成或孵育一定时间后,转移单元50通过孵育转移位(13a1-3、13b1-3、13c1-3中的一个)将反应容器从反应盘11内三圈11a、11b或11c的反应容器位移出、再通过清洗分离转移位(13d1或13d2)将反应容器移入反应盘11外圈11d的反应容器位。反应盘11每隔固定时间旋转前进1个位置,转送外圈11d反应容器位上的反应容器到清洗分离装置16。若反应容器已经孵育完成,则在转送过程中不需在外圈11d上继续孵育,若反应容器孵育未完成,则在转送到清洗分离装置16的过程中完成余下时间的孵育。外圈11d反应容器位上的反应容器经过清洗分离装置16的磁场时,由清洗分离装置16的冲洗机构和混匀机构对反应容器完成吸液、注清洗缓冲液、清洗混匀直至完成清洗分离。Step 204: Washing and separating: After the incubation is completed or after a certain period of incubation, the transfer unit 50 passes the transfer position (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) from the reaction tray 11 three times 11a The reaction vessel of 11b or 11c is displaced, and the reaction vessel is moved to the reaction vessel position of the outer ring 11d of the reaction disk 11 by washing and separating the transfer sites (13d 1 or 13d 2 ). The reaction disk 11 is rotated one position at a fixed time, and the reaction container on the outer ring 11d reaction container position is transferred to the cleaning and separating device 16. If the reaction vessel has been incubated, no incubation is required on the outer ring 11d during the transfer. If the reaction vessel incubation is not completed, the remaining time incubation is completed during the transfer to the wash separation device 16. When the reaction vessel on the outer ring 11d reaction vessel passes through the magnetic field of the cleaning and separating device 16, the washing mechanism and the mixing mechanism of the washing and separating device 16 complete the liquid absorption, the washing buffer, and the washing and mixing until the cleaning separation is completed. .
步骤205加注信号试剂:清洗分离完成后,反应盘11转送外圈11d反应容器位上的反应容器离开磁场区域,由清洗分离机构上耦合的信号试剂注液机构向反应容器内注入全部或部分信号试剂。Step 205 is filled with a signal reagent: after the cleaning separation is completed, the reaction tray 11 is transferred to the outer ring 11d. The reaction container at the reaction container is separated from the magnetic field region, and the signal reagent injection mechanism coupled by the cleaning and separating mechanism injects all or part of the reaction container into the reaction container. Signal reagent.
步骤206信号孵育:若需要信号孵育,则在反应盘11转送外圈11d上的该反应容器到测量装置86的过程中完成信号孵育,若不需要信号孵育,则该步骤省略。Step 206 Signal Incubation: If signal incubation is desired, signal incubation is accomplished during transfer of the reaction vessel 11 to the reaction vessel on the outer race 11d to the measurement device 86. This step is omitted if no signal incubation is required.
步骤207测量:需要测量的反应容器在外圈11d上转送到测量单元86后,视情况若需要则注入全部或部分信号试剂,由测量装置86对反应容器内的反应信号进行测量,测量结果经处理后传送至自动分析装置的控制中心。Step 207: The reaction container to be measured is transferred to the measuring unit 86 on the outer ring 11d, and if necessary, all or part of the signal reagent is injected, and the reaction signal in the reaction container is measured by the measuring device 86, and the measurement result is processed. It is then sent to the control center of the automatic analyzer.
步骤208丢弃反应容器:反应盘11继续转送外圈11d上的该反应容器到清洗分离转移位(13d1或13d2),转移单元50将测量后的反应容器移出反应盘,转移到丢弃反应容器孔60丢弃。Step 208 discards the reaction vessel: the reaction tray 11 continues to transfer the reaction vessel on the outer ring 11d to the washing separation transfer position (13d 1 or 13d 2 ), and the transfer unit 50 removes the measured reaction vessel from the reaction tray and transfers to the discarded reaction vessel. Hole 60 is discarded.
参考附图4和附图6,延时一步法测试流程和步骤与一步法试主要不同之处在于步骤301-305,将试剂分二次分注和增加了一次孵育,其他步骤与一步法类似,不再赘述。Referring to Figure 4 and Figure 6, the one-step one-step test procedure and procedure differs from the one-step test in that steps 301-305 are used to separate the reagents and add one incubation. The other steps are similar to the one-step method. ,No longer.
步骤301加注样本和第一试剂:加注单元20分别从吸样本位和吸试剂位吸取样本和第一试剂加注到加注站90上的反应容器内。Step 301 fills the sample and the first reagent: the filling unit 20 injects the sample and the first reagent from the suction sample position and the suction reagent position into the reaction container on the filling station 90, respectively.
步骤302混匀:若需要混匀,若需要混匀,则混匀机构对反应容器内的样本和试剂进
行混匀。若不需要混匀,则省略该步骤。Step 302: Mix well: If mixing is required, if mixing is required, the mixing mechanism feeds the sample and reagents in the reaction vessel.
Mix well. If no mixing is required, this step is omitted.
步骤303第一次孵育:转移单元50从加注站90将加注完样本和试剂的反应容器通过孵育转移位(13a1-3、13b1-3、13c1-3中的一个)转移到反应盘11内三圈11a、11b、11c上的某个反应容器位,反应容器开始在反应盘上孵育。反应容器孵育的同时,每隔固定时间随反应盘11旋转前进1个位置。孵育时间因具体测试项目而异,一般为5-60分钟。Step 303 First incubation: Transfer unit 50 transfers the reaction container filled with sample and reagent from the filling station 90 through the incubation transfer position (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) to One of the three reaction chambers 11a, 11b, 11c has a reaction vessel position, and the reaction vessel begins to incubate on the reaction tray. While the reaction vessel is being incubated, the reaction disk 11 is rotated one position at a fixed time. The incubation time varies depending on the specific test item, typically 5 to 60 minutes.
步骤304加注第二试剂:第一次孵育结束后,转移单元50将反应容器通过孵育转移位(13a1-3、13b1-3、13c1-3中的一个)从反应盘11内三圈11a、11b、11c上的反应容器位转移到加注站90上的反应容器位上,加注单元20从吸试剂位吸取第二试剂加注到加注站90上的反应容器内。Step 304: filling the second reagent: after the first incubation is completed, the transfer unit 50 passes the reaction vessel through the incubation transfer position (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) from the reaction tray 11 The reaction vessel locations on the loops 11a, 11b, 11c are transferred to the reaction vessel location on the fill station 90, and the fill unit 20 draws the second reagent from the draw reagent station into the reaction vessel on the fill station 90.
步骤305混匀:若需要混匀,若需要混匀,则混匀机构对反应容器内的样本和试剂进行混匀。若不需要混匀,则省略该步骤。Mixing in step 305: If mixing is required, if mixing is required, the mixing mechanism mixes the sample and reagents in the reaction vessel. If no mixing is required, this step is omitted.
参考附图4和附图7,两步法测试流程和步骤与延时一步法试主要不同之处在于增加了步骤404,增加了一次清洗分离。Referring to Figure 4 and Figure 7, the two-step test procedure and steps differ primarily from the one-step delay test in that step 404 is added to add a wash separation.
步骤404清洗分离:第一次孵育完成或第一次孵育一定时间后,转移单元50通过孵育转移位(13a1-3、13b1-3、13c1-3中的一个)将反应容器从反应盘11内三圈11a、11b或11c的反应容器位移出、再通过清洗分离转移位(13d1或13d2)将反应容器移入反应盘11外圈11d的反应容器位。反应盘11每隔固定时间旋转前进1个位置,转送外圈11d反应容器位上的反应容器到清洗分离装置16。若反应容器已经孵育完成,则在转送过程中不需在外圈11d上继续孵育,若反应容器孵育未完成,则在转送到清洗分离装置16的过程中完成余下时间的孵育。外圈11d反应容器位上的反应容器经过清洗分离装置16的磁场时,由清洗分离装置16的冲洗机构和混匀机构对反应容器完成吸液、注清洗缓冲液、清洗混匀直至完成第一次清洗分离。第一次清洗分离完成后,转移单元50将反应容器通过孵育转移位(13a1-3、13b1-3、13c1-3中的一个)从反应盘11内三圈11a、11b、11c上的反应容器位转移到加注站90上的反应容器上,加注单元20从吸试剂位吸取第二试剂加注到加注站90上的反应容器内。Step 404: Washing separation: After the first incubation is completed or after the first incubation for a certain period of time, the transfer unit 50 removes the reaction vessel from the reaction by incubating the transfer sites (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) The reaction vessel of the three turns 11a, 11b or 11c in the tray 11 is displaced, and the reaction vessel is moved into the reaction vessel position of the outer ring 11d of the reaction disk 11 by the washing separation transfer position (13d 1 or 13d 2 ). The reaction disk 11 is rotated one position at a fixed time, and the reaction container on the outer ring 11d reaction container position is transferred to the cleaning and separating device 16. If the reaction vessel has been incubated, no incubation is required on the outer ring 11d during the transfer. If the reaction vessel incubation is not completed, the remaining time incubation is completed during the transfer to the wash separation device 16. When the reaction vessel on the outer ring 11d reaction vessel passes through the magnetic field of the cleaning and separating device 16, the washing mechanism and the mixing mechanism of the washing and separating device 16 complete the liquid absorption, the cleaning buffer, and the washing and mixing until the first completion is completed. Separate cleaning. After the first washing separation is completed, the transfer unit 50 passes the reaction vessel through the incubation transfer position (one of 13a 1-3 , 13b 1-3 , 13c 1-3 ) from the three turns 11a, 11b, 11c in the reaction disk 11. The reaction vessel position is transferred to a reaction vessel on the filling station 90, and the filling unit 20 draws a second reagent from the aspirating reagent site into the reaction vessel on the filling station 90.
两步法其他步骤与延时一步法类似,不再赘述。The other steps of the two-step method are similar to the one-step method of delay, and will not be described again.
由以上描述可见,本实施例中,自动分析装置100先在内三圈集中孵育完成或孵育一定时间,孵育完成或孵育一定时间的反应容器再转移到外圈进行余下时间的孵育、完成清洗分离和测量,反应容器在不同圈之间的转移由转移单元通过设置在反应单元上的至少一个孵育转移位和一个清洗分离转移位完成,不仅节省了现有技术采用的独立清洗分离盘和测光盘,缩减了整机尺寸和降低了成本,还精简了测试步骤和降低了控制的复杂度和难度,避免了反应容器在多个盘之间的转移。此外,反应单元通过设置不同的转移位,可很好调整、设置和平衡内外圈反应容器位的数量,不仅可以实现灵活的孵育时间,还可以充分利用反应盘的内部空间,从而进一步缩减反应单元的尺寸,使整机结构更加紧凑,成本更低,测试效率更高。As can be seen from the above description, in the present embodiment, the automatic analysis device 100 is first concentrated or incubated for a certain period of time in the inner three rounds, and the reaction container that has been incubated or incubated for a certain period of time is transferred to the outer ring for the remaining time to complete the cleaning and separation. And measuring, the transfer of the reaction vessel between different circles is completed by the transfer unit through at least one incubation transfer position and one cleaning separation transfer position disposed on the reaction unit, which not only saves the independent cleaning separation disk and the optical disk used in the prior art. , reducing the size of the machine and reducing the cost, but also streamlining the test steps and reducing the complexity and difficulty of the control, avoiding the transfer of the reaction vessel between multiple disks. In addition, the reaction unit can adjust, set and balance the number of reaction vessels in the inner and outer ring by setting different transfer positions, which not only can realize flexible incubation time, but also fully utilize the internal space of the reaction disk, thereby further reducing the reaction unit. The size makes the whole structure more compact, lower cost and more efficient.
本发明的第二种实施例,参见图8。本实施例中的样本输送单元30、试剂存储单元40和加注单元20与实施例一相同或形似,不再赘述。本实施例中,转移单元50设置为1个,可做二维运动,这样可使整机更为紧凑和成本更低。转移单元50包括Y向导轨50a、Y向运动机械臂50b以及垂直运动机构和机械手指(图中未标出)等机构。转移单元50可沿着Y向水平移动机械手指,水平运动范围为一维线性区域56,可将反应容器在反应容器供给单元70、反应单元10上的2个孵育转移位(13b、13c)、反应单元10上的1个清洗分离转移位(13a)、反应单元10上的1个测量转移位(13d)、丢反应容器位60之间转移。此外,由于转移单元50运动范围覆盖反应单元10上的多个孵育转移位,转移单元可以通过不同的孵育转移位移入反应容器或从不同的孵育转移位转移出应容器来实现灵活的孵
育时间。加注站90与实施例一的主要不同在于其可水平运动,可以沿着X向水平运动到转移单元50的水平运动范围内。反应容器供给单元70与实施例一的主要不同之处在于其上的反应容器只有一列在转移单元50的水平运动范围内,为了持续供给反应容器,反应容器供给单元70可沿着X向水平运动,以便使其上的各列反应容器经过转移单元50的水平运动范围,这样转移单元50可以遍历托盘上每个反应容器位上的未使用的反应容器,为新开始的测试提供未使用的反应容器。反应单元与实施例一的主要不同在清洗分离装置的布置以及转移位的设置上。本实施例中,清洗分离装置16布置在反应盘的内圈11a、对通过清洗分离转移位13a进入反应盘内圈11a上的反应容器进行清洗分离。测量装置86安装在保温装置侧面上,对通过测量转移位13d进入反应盘外圈11d上的反应容器内的信号进行测量。中间两圈11b、11c上的反应容器位对通过孵育转移位(13b、13c)进入处理单元的反应容器进行孵育。本实施例中,对应转移单元50的水平一维运动范围和反应盘四圈11a、11b、11c、11d上的反应容器位的相交点,在反应单元上由内向外依次设置清洗分离转移位13a、孵育转移位13b和13c、测量转移位13d共4个转移位。本实施将清洗分离装置布置在反应单元内圈上,不仅可使清洗分离装置更紧凑,还减少了清洗分离装置对测量可能造成的温度波动、引入环境光的干扰等不利影响。A second embodiment of the invention is shown in FIG. The sample transport unit 30, the reagent storage unit 40, and the filling unit 20 in this embodiment are the same as or similar to those in the first embodiment, and will not be described again. In this embodiment, the transfer unit 50 is set to one, which can perform two-dimensional motion, which makes the whole machine more compact and lower in cost. The transfer unit 50 includes a Y-direction guide rail 50a, a Y-direction moving robot arm 50b, and a mechanism such as a vertical motion mechanism and a mechanical finger (not shown). The transfer unit 50 can move the mechanical finger horizontally along the Y direction, the horizontal movement range is a one-dimensional linear region 56, and the two reaction transfer positions (13b, 13c) of the reaction container on the reaction container supply unit 70 and the reaction unit 10 can be One wash separation transfer position (13a) on the reaction unit 10, one measurement transfer position (13d) on the reaction unit 10, and a lost reaction container position 60 are transferred. In addition, since the range of motion of the transfer unit 50 covers a plurality of incubation transfer sites on the reaction unit 10, the transfer unit can be transferred into the reaction vessel through different incubation shifts or transferred from the different incubation transfer positions to achieve flexible incubation.
Breeding time. The main difference between the filling station 90 and the first embodiment is that it can move horizontally and can move horizontally along the X direction to the horizontal range of motion of the transfer unit 50. The main difference between the reaction vessel supply unit 70 and the first embodiment is that only one column of the reaction vessel thereon is in the horizontal movement range of the transfer unit 50. In order to continuously supply the reaction vessel, the reaction vessel supply unit 70 can move horizontally along the X direction. So that the columns of reaction vessels above it pass the horizontal range of motion of the transfer unit 50, such that the transfer unit 50 can traverse the unused reaction vessels on each of the reaction vessel locations on the tray to provide an unused reaction for the newly initiated test. container. The main difference between the reaction unit and the first embodiment is in the arrangement of the cleaning separation device and the setting of the transfer position. In the present embodiment, the cleaning and separating device 16 is disposed in the inner ring 11a of the reaction disk, and cleans and separates the reaction container that has entered the reaction disk inner ring 11a by the cleaning separation transfer position 13a. The measuring device 86 is mounted on the side of the heat retaining device and measures the signal in the reaction vessel that has entered the outer ring 11d of the reaction disk by measuring the transfer position 13d. The reaction vessel positions on the middle two turns 11b, 11c are incubated with the reaction vessel entering the treatment unit by incubation of the transfer sites (13b, 13c). In this embodiment, corresponding to the horizontal one-dimensional motion range of the transfer unit 50 and the intersection point of the reaction container positions on the four turns 11a, 11b, 11c, and 11d of the reaction disk, the cleaning separation transfer bit 13a is sequentially disposed from the inside to the outside on the reaction unit. The transfer sites 13b and 13c were incubated, and the transfer sites 13d were measured for a total of 4 transfer sites. In this embodiment, the cleaning and separating device is arranged on the inner ring of the reaction unit, which not only makes the cleaning and separating device more compact, but also reduces the adverse effects of the cleaning and separating device on the temperature fluctuations that may be caused by the measurement and the interference of introducing ambient light.
本领域内普通技术人员可以理解,本实施例的测试流程和步骤与实施例一相似,故以下只做简单描述。测试时,加注站90沿X向水平运动到转移单元50水平运动范围内,转移单元50从反应容器供给单元70转移一个未使用反应容器放到加注站90的反应容器位上,然后加注站90运动到加注单元20的水平运动轨迹下,加注单元20向加注站90上的反应容器加注样本和试剂,加注完成后,集成于加注站90的混匀器可以对反应容器进行混匀。混匀完成后或混匀过程中,加注站90再次水平运动到转移单元50的水平运动范围内。这样,加注站90上需要孵育的反应容器先由转移单元50通过孵育转移位13b或13c移入中间两圈11b、11c中的一圈,孵育完成或孵育一定时间后需要清洗分离时再由转移单元50通过孵育转移位13b或13c移出中间两圈11b、11c、再通过清洗分离转移位13a移入内圈11a,在反应盘的旋转转送下,由清洗分离装置86进行清洗分离,清洗分离完成,则由转移单元50通过清洗分离转移位13a移出内圈11d,若需要加第二试剂,则转移单元50将反应容器转移到加注站90完成第二试剂的加注;若需要测量,则由转移单元50通过测量转移位13d移入外圈11d,反应容器在反应盘的旋转下,转送到测量装置进行测量。It will be understood by those skilled in the art that the test procedure and steps of this embodiment are similar to those of the first embodiment, and therefore only a brief description will be given below. At the time of the test, the filling station 90 is moved horizontally in the X direction to the horizontal movement range of the transfer unit 50, and the transfer unit 50 transfers an unused reaction container from the reaction container supply unit 70 to the reaction container position of the filling station 90, and then adds The filling station 90 moves to the horizontal movement track of the filling unit 20, and the filling unit 20 fills the reaction container on the filling station 90 with the sample and the reagent. After the filling is completed, the mixer integrated in the filling station 90 can be Mix the reaction vessel. After the mixing is completed or during the mixing process, the filling station 90 is again horizontally moved to the horizontal movement range of the transfer unit 50. Thus, the reaction vessel to be incubated on the filling station 90 is first transferred by the transfer unit 50 through the incubation transfer position 13b or 13c into one of the middle two loops 11b, 11c, and after the incubation is completed or after a certain period of time, it is necessary to wash and separate and then transfer. The unit 50 moves out of the middle two rings 11b, 11c by the incubation transfer position 13b or 13c, and then moves into the inner ring 11a through the cleaning separation transfer position 13a, and is cleaned and separated by the cleaning and separating device 86 under the rotation transfer of the reaction disk, and the cleaning and separation are completed. Then, the transfer unit 30 removes the inner ring 11d by the cleaning separation transfer position 13a. If the second reagent needs to be added, the transfer unit 50 transfers the reaction container to the filling station 90 to complete the filling of the second reagent; if measurement is required, The transfer unit 50 is moved into the outer ring 11d by measuring the transfer position 13d, and the reaction container is transferred to the measuring device for measurement under the rotation of the reaction disk.
本发明的第三种实施例,参见图9。本实施与实施一主要不同在于测量装置的布置上。本实施例还包括测量暗室(图中未标出)和独立于反应单元10的测量位82,测量装置86安装于测量暗室上,对测量位82上的的反应容器内的信号进行测量。测量暗室为测量装置86提供所需的暗室环境,测量位82在转移单元50的水平运动范围内或可水平运动到所述转移单元50的水平运动范围内。为了容易实现避光,测量位82可以做成固定位置,反应容器的进出口设置“天窗”机构,平时关闭以保证测量暗室的暗室环境,反应容器进出时打开;测量位82也可以做成移动位置,为了容易避光,测量位82可以以推拉抽屉等形式远离或靠近测量装置86。当然测量位82及相应的避光结构可以是其他合适的实现方式。此外,信号试剂的加注也可在测量位82完成。该实施例可以使测量装置86相对独立,更容易实现测量时的密闭暗室环境,而反应单元不需要再设置专门针对测量装置86避光要求的结构。本领域内普通技术人员可以理解,本实施的其它单元与实施例一相同或相似,本实施例的测试流程和步骤参考图5、图6和图7,与实施例一的主要不同在最后的加注信号试剂、测量、丢弃反应容器三个步骤,其余相同或相似。本实施例中的加注信号试剂步骤可以在反应盘外圈11d的反应容器位上完成,也可以在测量位82完成,还可以在反应盘外圈11d的反应容器位上完成第一信号试剂的加注,在测量位82完成第二信号试剂的加注;测量步骤,转移单元50将需要测量的反应容器通过清洗分离转移位13d1或13d2
从反应盘外圈11d的反应容器位转移到测量位82,由测量装置86对位于测量位82的反应容器内的反应信号进行测量;丢弃反应容器步骤,转移单元50将完成测量的反应容器从测量位82转移到丢弃孔60丢弃。A third embodiment of the present invention is shown in FIG. This embodiment differs from the first embodiment in the arrangement of the measuring device. This embodiment also includes measuring a darkroom (not shown) and a measurement location 82 independent of the reaction unit 10, and the measurement device 86 is mounted on the measurement darkroom to measure the signal in the reaction vessel on the measurement site 82. The measurement darkroom provides the desired darkroom environment for the measurement device 86, which is within the horizontal range of motion of the transfer unit 50 or can be moved horizontally to the horizontal range of motion of the transfer unit 50. In order to avoid light, the measuring position 82 can be made into a fixed position, and the inlet and outlet of the reaction container are provided with a "sunroof" mechanism, which is normally closed to ensure the measurement of the darkroom environment of the darkroom, and the reaction container is opened when it enters and exits; the measuring position 82 can also be made mobile. The position, in order to be easily protected from light, the measuring position 82 can be moved away from or close to the measuring device 86 in the form of a push-pull drawer or the like. Of course, the measurement bit 82 and the corresponding light-shielding structure may be other suitable implementations. In addition, the filling of the signal reagent can also be done at measurement position 82. This embodiment makes the measuring device 86 relatively independent, and it is easier to realize a closed darkroom environment during measurement, and the reaction unit does not need to be provided with a structure specifically for the light-shielding requirement of the measuring device 86. It will be understood by those skilled in the art that other units of the present embodiment are the same as or similar to the first embodiment. The test flow and steps of this embodiment refer to FIG. 5, FIG. 6, and FIG. 7, which are mainly different from the first embodiment. Fill the signal reagent, measure, and discard the reaction vessel in three steps, the same or similar. The step of filling the signal reagent in the embodiment may be completed on the reaction vessel position of the outer ring 11d of the reaction disk, or may be completed at the measurement position 82, and the first signal reagent may be completed on the reaction vessel position of the outer ring 11d of the reaction disk. Filling, the filling of the second signal reagent is completed at the measuring position 82; in the measuring step, the transfer unit 50 transfers the reaction container to be measured through the cleaning separation transfer position 13d 1 or 13d 2 from the reaction container position of the outer disk 11d of the reaction disk To measurement location 82, the reaction signal in reaction vessel located at measurement location 82 is measured by measurement device 86; the reaction vessel step is discarded, and transfer unit 50 transfers the measurement vessel from which measurement is completed from measurement location 82 to disposal aperture 60.
本发明的第四种实施例,参见图10。本实施例与实施一主要不同在还包括独立于反应单元10的测量暗室82和测量盘81,测量装置86安装于测量暗室82上。本实施例中,加注站(图中未标出)可以集成在测量盘81,可以充分利用测量盘81的反应容器位以及其旋转定位功能,这样可以省去设置独立的加注站,节省了机构,可以使整机成本更低,结构更为紧凑。混匀机构可集成于加注站,用于对加注后的反应容器进行超声混匀或震荡混匀。测量盘81上设置以测量盘旋转中心为圆心的一圈反应容器位81a,用于承载需要进行测量的反应容器。本实施例设置多个反应容器位,可以实现全部或部分信号孵育。测量盘81每次旋转,可把任一信号孵育位上的反应容器旋转到测量装置86进行测量,从而实现灵活的信号孵育,提高测试的灵活性和效率。为了反应容器进出测量盘81,测量暗室82的上部设置测量转移位82a。测量转移位82a在转移单元50的水平运动范围内,转移单元50可将需要测量的反应容器通过反应单元10的清洗分离转移位13d1或13d2从反应盘11移出、通过测量转移位82a移入测量盘81。测量暗室82包裹或包围在测量盘81的周边,为测量装置86提供暗室环境,对于信号孵育的测试,测量暗室82侧面或底部可以选择设置加热装置和传感器,为测量盘反应容器位81a提供恒温信号孵育环境。测量装置86包括微弱光探测器光电倍增管(PMT),直接安装在测量暗室82上,对反应容器内加入信号试剂后产生的微弱化学发光信号进行测量。此外,为了方便信号试剂的加注,本发明的测量盘81上部或测量暗室82的外围,还可设置信号试剂加注机构(图中未标出),向测量盘81反应容器位上的反应容器内加注全部或部分信号试剂。本领域内普通技术人员可以理解,本实施的其它单元与实施例一相同或相似,本实施例的测试流程和步骤参考图5、图6和图7,与实施例一的主要不同在最初的加载反应容器、加注样本和试剂以及最后的加注信号试剂、测量、丢弃反应容器三个步骤等,其余相同或相似。转移单元50将未使用的反应容器从反应容器供给单元70通过测量转移位82a移入测量盘81上的反应容器位,测量盘81旋转,将反应容器转送到加注站,加注单元20吸取样本和试剂加注到位于加注站上的反应容器,加注完成后,集成于加注站的混匀机构对反应容器内的混合物进行混匀。混匀完成后,转移单元50将完成测量的反应容器通过测量转移位82a从测量盘81上的反应容器位转移到反应单元孵育。本实施例中的加注信号试剂步骤可以在反应盘外圈11d的反应容器位上完成,也可以在测量盘81上的反应容器位完成,还可以在反应盘外圈11d的反应容器位上完成第一信号试剂的加注,在测量盘81上的反应容器位完成第二信号试剂的加注;测量步骤,转移单元50将需要测量的反应容器通过清洗分离转移位13d1或13d2从反应盘外圈11d的反应容器位移出、通过测量转移位82a移入测量盘81上的反应容器位,测量盘81旋转,将反应容器转送到测量装置86,由测量装置86对反应容器内的反应信号进行测量;丢弃反应容器步骤,转移单元50将完成测量的反应容器通过测量转移位82a从测量盘81上的反应容器位转移到丢弃孔60丢弃。A fourth embodiment of the invention is shown in FIG. This embodiment differs from the first embodiment in that it further includes a measuring dark chamber 82 and a measuring disk 81 which are independent of the reaction unit 10, and the measuring device 86 is mounted on the measuring dark room 82. In this embodiment, the filling station (not shown) can be integrated in the measuring disc 81, and the reaction vessel position of the measuring disc 81 and its rotational positioning function can be fully utilized, so that the independent filling station can be omitted, saving The mechanism can make the whole machine cost less and the structure is more compact. The mixing mechanism can be integrated into the filling station for ultrasonic mixing or shaking mixing of the filled reaction vessel. A measuring reaction vessel position 81a is provided on the measuring disk 81 to measure the center of rotation of the disk for carrying the reaction vessel to be measured. In this embodiment, a plurality of reaction vessel positions are provided, and all or part of the signal incubation can be achieved. Each time the measuring disc 81 is rotated, the reaction vessel at any of the signal incubation positions can be rotated to the measuring device 86 for measurement, thereby achieving flexible signal incubation and improving test flexibility and efficiency. In order to allow the reaction container to enter and exit the measuring disk 81, the measurement transfer position 82a is set in the upper portion of the measurement dark room 82. The measurement transfer position 82a is within the horizontal movement range of the transfer unit 50, and the transfer unit 50 can remove the reaction container to be measured from the reaction disk 11 through the cleaning separation transfer position 13d 1 or 13d 2 of the reaction unit 10, and move it in by the measurement transfer position 82a. The disc 81 is measured. The measurement dark chamber 82 is wrapped or surrounded by the periphery of the measuring disc 81 to provide a darkroom environment for the measuring device 86. For the signal incubation test, the heating device and the sensor may be selectively disposed on the side or the bottom of the measuring dark chamber 82 to provide a constant temperature for the measuring disc reaction container position 81a. Signal incubation environment. The measuring device 86 includes a weak photodetector photomultiplier tube (PMT) directly mounted on the measuring dark chamber 82 to measure a weak chemiluminescence signal generated by adding a signal reagent to the reaction vessel. In addition, in order to facilitate the filling of the signal reagent, the upper portion of the measuring disk 81 of the present invention or the periphery of the measuring dark room 82 may be provided with a signal reagent filling mechanism (not shown) to react to the reaction position of the measuring disk 81. Fill all or part of the signal reagent in the container. It will be understood by those skilled in the art that other units of the present embodiment are the same as or similar to the first embodiment. The test flow and steps of the present embodiment are different from those of the first embodiment with reference to FIG. 5, FIG. 6, and FIG. Loading the reaction vessel, filling the sample and reagents, and finally adding the signal reagent, measuring, discarding the reaction vessel, and the like, the others are the same or similar. The transfer unit 50 moves the unused reaction container from the reaction container supply unit 70 through the measurement transfer position 82a to the reaction container position on the measuring disk 81, the measurement disk 81 is rotated, the reaction container is transferred to the filling station, and the filling unit 20 sucks the sample. The reagent is filled into the reaction vessel at the filling station. After the filling is completed, the mixing mechanism integrated in the filling station mixes the mixture in the reaction vessel. After the completion of the mixing, the transfer unit 50 transfers the measurement container that has completed the measurement from the reaction vessel position on the measurement disk 81 to the reaction unit by measuring the transfer position 82a. The step of filling the signal reagent in the embodiment may be completed at the reaction vessel position of the outer ring 11d of the reaction disk, or may be completed at the reaction vessel position on the measuring disk 81, or may be at the reaction vessel position of the outer ring 11d of the reaction disk. The filling of the first signal reagent is completed, and the filling of the second signal reagent is completed in the reaction container position on the measuring disk 81; in the measuring step, the transfer unit 50 passes the reaction container to be measured through the cleaning separation transfer position 13d1 or 13d2 from the reaction disk. The reaction vessel of the outer ring 11d is displaced, moved into the reaction vessel position on the measuring disk 81 by the measuring transfer position 82a, the measuring disk 81 is rotated, and the reaction vessel is transferred to the measuring device 86, and the reaction signal in the reaction vessel is performed by the measuring device 86. The reaction vessel step is discarded, and the transfer unit 50 transfers the measurement container that has completed the measurement from the reaction vessel position on the measuring disk 81 to the disposal hole 60 by the measurement transfer position 82a.
本发明的自动分析装置还可以灵活拓展和最大限度地复用,实现产品的系列化。在实施例四的基础上,为了进一步提升整机规格参数和测试通量,满足标本量更大的终端客户需求,可以通过增加转移单元和加注单元数量、适当增大反应单元尺寸或增加反应单元数量等方式来实现。参考图11为本发明自动分析装置的第五种实施方式示意图。样本输送单元30采取轨道和样本架的进样方式,这样可以容纳更多样本,可以实时追加样本,操作也更为方便。样本架32和其上的样本管31可被输送到第一加注单元21的运动范围下。试剂存储单元40增加了试剂存放位置,可以放置更多试剂容器。加注单元20包括第一加注单元21和第二加注单元22,第一加注单元21只加注样本或加注样本和部分试剂,第二
加注单元22加注试剂,当然也可增加更多的加注单元,这样提高了加样本和试剂的速度。反应容器供给单元70采用料仓式,反应容器可以成包散乱倒入反应容器供给单元70的料仓中,这种方式可使反应容器的供给更多、更快、更方便。反应单元10的反应盘11包括以反应盘旋转中心为圆心分布的外圈11d反应容器位和内部区域11a反应容器位。内部区域11a上的反应容器位,成“蜂窝状”分布,这样可以充分利用反应盘11上的空间,设置更多的反应容器位,容纳更多的反应容器孵育,提升测试通量。为了反应容器进出反应盘11上的反应容器位,反应单元10上设置一个孵育转移区13a(包括7个孵育转移位)和清洗分离转移位13d。测量暗室82和测量盘81以及测量装置86可以完全复用实施例四,但为了提高测试效率,不再设置加注位。测量暗室82上设置测量转移位82a,供反应容器进出测量盘。转移单元50包括可独立做三维运动的第一转移单元51和第二转移单元52,第一反应容器单元51主要在反应单元10的孵育转移区13a和清洗分离转移位13d、测量盘81以及反应容器丢弃孔60b等位置之间转移反应容器,第二转移单元52主要在反应容器供给单元70、加注站90、反应单元10的孵育转移区13a和清洗分离转移位13d以及反应容器丢弃孔60b之间转移反应容器。本领域普通技术人员可以理解,通过合理的布局和分配,任意两个位置之间反应容器的转移都可通过第一或第二转移单元或两者同时完成。当然,转移单元可以不止2个,可以根据需要设置更多的转移单元以提高反应容器转移的效率和速度。为了整机布局紧凑和提高测试速度,本实施例采用独立加注站90的方式加注样本和试剂。加注站90可在反应容器供给单元70、第一加注单元21、第二加注单元22之间来回移动,接收反应容器供给单元70供给的反应容器、接受第一加注单元21加注样本或样本和部分试剂、接受第二加注单元22加注试剂。可以在加注站90上或加注单元20上集成混匀机构,对加注样本或/和试剂后的反应容器进行混匀。混匀完成后,加注站90上的反应容器由转移单元50转移到反应单元10。本领域内普通技术人员可以理解,本实施的其它单元与实施例四相同或相似,本实施例的测试流程和步骤与实施例一主要不同在于加注样本和试剂由第一和第二加注单元协调配合完成,反应容器转移由第一和第二转移单元协调配合完成,加注单元的加注动作在独立的加注站完成,其它动作和流程与实施例一相同或相似,参考图5-图7,不再赘述。该实施例与现有技术相比,避免了额外的大尺寸的清洗分离盘,独立于反应单元的的测量装置更容易实现暗室环境和灵活的测量,同时通过功能不同的反应容器位的分区也减少了反应单元自身的尺寸,从而使整机更为紧凑、成本更低、效率更高和可靠性更好。The automatic analysis device of the invention can also be flexibly expanded and maximized to achieve serialization of products. On the basis of the fourth embodiment, in order to further improve the specification parameters and test throughput of the whole machine and meet the needs of end users with larger specimens, the number of transfer units and filling units can be increased, the size of the reaction unit can be appropriately increased, or the reaction can be increased. The number of units is used to achieve this. Referring to Figure 11, there is shown a schematic view of a fifth embodiment of the automatic analyzer of the present invention. The sample transport unit 30 adopts the injection mode of the track and the sample rack, so that more samples can be accommodated, the sample can be added in real time, and the operation is more convenient. The sample holder 32 and the sample tube 31 thereon can be delivered to the range of motion of the first filling unit 21. The reagent storage unit 40 increases the reagent storage position and allows more reagent containers to be placed. The filling unit 20 includes a first filling unit 21 and a second filling unit 22, the first filling unit 21 only filling the sample or filling the sample and the partial reagent, the second
The filling unit 22 is filled with reagents, and of course more filling units can be added, which increases the speed of adding the sample and the reagent. The reaction vessel supply unit 70 adopts a silo type, and the reaction vessel can be poured into a silo of the reaction vessel supply unit 70 in a scattered manner, which makes the supply of the reaction vessel more, faster, and more convenient. The reaction disk 11 of the reaction unit 10 includes an outer ring 11d reaction vessel position and an inner region 11a reaction vessel position distributed centering on the center of rotation of the reaction disk. The reaction vessel position on the inner region 11a is distributed in a "honeycomb" manner, so that the space on the reaction disk 11 can be fully utilized, more reaction vessel positions can be set, more reaction vessels can be accommodated, and the test throughput can be increased. In order to allow the reaction vessel to enter and exit the reaction vessel position on the reaction disk 11, an incubation transfer zone 13a (including 7 incubation transfer sites) and a wash separation transfer site 13d are disposed on the reaction unit 10. The measurement dark room 82 and the measuring disk 81 and the measuring device 86 can be completely multiplexed with the fourth embodiment, but in order to improve the test efficiency, the filling bit is no longer set. A measurement transfer position 82a is provided on the measurement dark chamber 82 for the reaction container to enter and exit the measurement disk. The transfer unit 50 includes a first transfer unit 51 and a second transfer unit 52 that can perform three-dimensional movement independently. The first reaction container unit 51 is mainly in the incubation transfer zone 13a of the reaction unit 10, the cleaning separation transfer position 13d, the measurement disk 81, and the reaction. The reaction container is transferred between the container discarding holes 60b and the like, and the second transfer unit 52 is mainly at the reaction container supply unit 70, the filling station 90, the incubation transfer zone 13a of the reaction unit 10, and the cleaning separation transfer position 13d and the reaction container disposal hole 60b. Transfer the reaction vessel between. One of ordinary skill in the art will appreciate that by reasonable placement and distribution, the transfer of the reaction vessel between any two locations can be accomplished simultaneously by the first or second transfer unit or both. Of course, there may be more than two transfer units, and more transfer units may be provided as needed to increase the efficiency and speed of transfer of the reaction vessel. In order to make the layout of the whole machine compact and to improve the test speed, the present embodiment uses the method of the independent filling station 90 to fill the sample and the reagent. The filling station 90 can move back and forth between the reaction container supply unit 70, the first filling unit 21, and the second filling unit 22, receive the reaction container supplied from the reaction container supply unit 70, and accept the filling of the first filling unit 21. The sample or sample and a portion of the reagent are received by the second filling unit 22. The mixing mechanism can be integrated on the filling station 90 or the filling unit 20 to mix the reaction container after the sample or/and the reagent is added. After the mixing is completed, the reaction vessel on the filling station 90 is transferred from the transfer unit 50 to the reaction unit 10. It will be understood by those skilled in the art that other units of the present embodiment are the same as or similar to the fourth embodiment. The test procedure and steps of the present embodiment are mainly different from the first embodiment in that the sample and reagent are filled by the first and second filling. The unit coordination is completed, the reaction container transfer is completed by the first and second transfer units, and the filling operation of the filling unit is completed at the independent filling station. The other actions and processes are the same or similar to those of the first embodiment. - Figure 7, no further details. Compared with the prior art, this embodiment avoids the extra large size of the cleaning separation disc, and the measurement unit independent of the reaction unit is easier to realize the darkroom environment and flexible measurement, and the partition of the reaction vessel position through different functions is also The size of the reaction unit itself is reduced, making the machine more compact, less costly, more efficient and more reliable.
本发明实施例还提供了一种样本分析方法,具体包括:The embodiment of the invention further provides a sample analysis method, which specifically includes:
加注步骤,在反应容器中加注样本和/或试剂;a filling step of filling a sample and/or reagent in the reaction vessel;
孵育步骤,对通过至少一个孵育转移位进入反应单元的至少包括需要孵育两次的反应容器进行孵育;An incubation step of incubating at least one reaction vessel that needs to be incubated twice by entering at least one incubation transfer site into the reaction unit;
清洗分离步骤,对通过至少一个清洗分离转移位进入反应单元的反应容器进行清洗分离,以去除反应物中未结合的成分;a washing and separating step of washing and separating the reaction vessel entering the reaction unit through at least one washing and separating transfer point to remove unbound components in the reactant;
加注信号试剂步骤,向反应容器内加注信号试剂,Filling the signal reagent reagent step, adding a signal reagent to the reaction vessel,
测量步骤,通过测量装置对反应容器内的反应信号进行测量。The measuring step measures the reaction signal in the reaction vessel by the measuring device.
进一步地,还包括转移步骤,通过转移单元从所述至少一个孵育转移位、至少一个清洗分离转移位将反应容器移入移出所述反应单元;还包括混匀步骤,对反应容器内的反应物混匀。Further, further comprising a transfer step of moving the reaction vessel into and out of the reaction unit from the at least one incubation transfer position by the transfer unit, at least one wash separation transfer position; further comprising a mixing step of mixing the reactants in the reaction vessel uniform.
本发明以反应单元为中心实现反应容器内反应物的孵育、清洗分离,反应单元上设置至少一个孵育转移位和至少一个清洗分离转移位,转移单元可在孵育转移位和清洗分离位之间转移反应容器,不仅可以实现灵活的孵育,而且还可以解决现有技术中必须采用多个清洗分离机构实现两步法测试的问题,充分实现高效的清洗分离。此外,测量装置可根据
整机布局或结构实现的需要,灵活安排或布置,比如可直接安装在反应单元上、设置在独立位置上或安装在独立测量盘上等,解决了现有技术中测量装置布置受限、测量环境易干扰等问题。本发明提高了分析装置的工作效率和降低了自动化功能的实现难度,很好解决了目前自动化仪器体积大、检测速度慢、成本高、性能差等技术难题,不但节约了实验室空间,提高了测试效率,而且有利于减少费用开支,减轻受测者负担,最终节约了大量的自然资源和社会资源。The invention realizes the incubation and washing separation of the reactants in the reaction vessel centering on the reaction unit, and at least one incubation transfer position and at least one cleaning separation transfer position are arranged on the reaction unit, and the transfer unit can be transferred between the incubation transfer position and the cleaning separation position. The reaction vessel can not only realize flexible incubation, but also solve the problem that the two-step test must be implemented by using multiple cleaning and separating mechanisms in the prior art, and fully realize efficient cleaning separation. In addition, the measuring device can be
The layout or structure of the whole machine needs to be flexibly arranged or arranged, for example, it can be directly installed on the reaction unit, set in a separate position or installed on an independent measuring plate, etc., which solves the limitation of the arrangement of the measuring device in the prior art and the measurement. The environment is prone to interference and other issues. The invention improves the working efficiency of the analysis device and reduces the difficulty of realizing the automatic function, and solves the technical problems of large volume, low detection speed, high cost and poor performance of the current automatic instrument, which not only saves the laboratory space, but also improves the laboratory space. Test efficiency, and help reduce expenses, reduce the burden on the testee, and ultimately save a lot of natural resources and social resources.
本发明实施例中描述的技术特征或操作步骤可以按照任何合适的方式进行组合。本领域内普通技术人员容易理解,本发明实施例描述的方法中的步骤或动作的顺序是可以改变的。因此,除非另有说明要求一定的顺序,在附图或者详细描述中的任何顺序只是为了用作说明的目的,而不是必须的顺序。The technical features or operational steps described in the embodiments of the present invention may be combined in any suitable manner. It will be readily understood by those skilled in the art that the order of the steps or actions in the methods described in the embodiments of the present invention can be changed. Therefore, any order in the drawings or the detailed description is merely for the purpose of illustration and not a
本发明的各实施例中可以包括各种步骤,这些步骤可以体现为可由通用或专用计算机(或其它电子设备)执行的机器可执行的指令。可选地,这些步骤可以由包括了用以执行这些步骤的特定逻辑电路的硬件元件执行或者由硬件、软件和/或固件联合执行。Various embodiments may be included in various embodiments of the invention, which may be embodied as machine-executable instructions that are executable by a general purpose or special purpose computer (or other electronic device). Alternatively, these steps may be performed by hardware elements comprising specific logic circuitry to perform the steps or jointly by hardware, software and/or firmware.
以上通过具体的实施例对本发明进行了说明,但本发明并不限于这些具体的实施例。本领域技术人员应该明白,还可以对本发明做各种修改、等同替换、变化等等,这些变换只要未背离本发明的精神,都应在本发明的保护范围之内。此外,以上多处所述的“一个实施例”“本实施例”等表示不同的实施例,当然也可以将其全部或部分结合在一个实施例中。The present invention has been described above by way of specific embodiments, but the invention is not limited to the specific embodiments. It will be apparent to those skilled in the art that various modifications, equivalents, changes, and the like may be made without departing from the spirit and scope of the invention. In addition, the "one embodiment", "this embodiment" and the like described above in various places represent different embodiments, and of course, all or part of them may be combined in one embodiment.
以上所述实施例仅表达了本发明的几种实施方式,其描述较为具体和详细,但并不能因此而理解为对本发明专利范围的限制。应当指出的是,对于本领域的普通技术人员来说,在不脱离本发明构思的前提下,还可以做出若干变形和改进,这些都属于本发明的保护范围。因此,本发明专利的保护范围应以所附权利要求为准。
The above-mentioned embodiments are merely illustrative of several embodiments of the present invention, and the description thereof is more specific and detailed, but is not to be construed as limiting the scope of the invention. It should be noted that a number of variations and modifications may be made by those skilled in the art without departing from the spirit and scope of the invention. Therefore, the scope of the invention should be determined by the appended claims.
Claims (10)
- 一种自动分析装置,其特征在于,包括:An automatic analysis device, comprising:加注单元,加注样本和/或试剂到反应容器;Filling unit, filling the sample and / or reagent into the reaction vessel;反应单元,孵育并清洗分离反应容器内的反应物;a reaction unit, incubating and washing the reactants in the separation reaction vessel;测量装置,测量反应容器内的反应信号;Measuring device for measuring a reaction signal in the reaction vessel;转移单元,在不同位置之间转移反应容器;a transfer unit that transfers the reaction vessel between different locations;所述反应单元包括一个旋转装置,所述旋转装置上设置反应容器位,用于承载和固定反应容器,所述反应单元上设置至少一个孵育转移位和至少一个清洗分离转移位,所述反应单元的至少一个孵育转移位和至少一个清洗分离转移位在转移单元的水平运动范围内。The reaction unit comprises a rotating device, wherein the rotating device is provided with a reaction vessel position for carrying and fixing the reaction vessel, and the reaction unit is provided with at least one incubation transfer position and at least one cleaning separation transfer position, the reaction unit At least one incubation transfer site and at least one wash separation transfer site are within the horizontal range of motion of the transfer unit.
- 根据权利要求1所述的自动分析装置,其特征在于,所述反应单元包括清洗分离装置,所述清洗分离装置对通过所述清洗分离转移位进入反应单元的反应容器进行清洗分离,以去除反应物中未结合的成分。The automatic analyzer according to claim 1, wherein the reaction unit comprises a washing and separating device that cleans and separates a reaction vessel that has entered the reaction unit through the washing and separating transfer point to remove the reaction. Unbound ingredients.
- 根据权利要求1所述的自动分析装置,其特征在于,通过所述孵育转移位进入反应单元的反应容器至少包括需要孵育两次的反应容器,所述反应单元对通过所述孵育转移位进入反应单元的反应容器进行孵育。The automatic analyzer according to claim 1, wherein the reaction vessel entering the reaction unit through the incubation transfer site comprises at least a reaction vessel that needs to be incubated twice, and the reaction unit enters a reaction by transferring the transfer site. The reaction vessel of the unit is incubated.
- 根据权利要求1所述的自动分析装置,其特征在于,所述旋转装置为反应盘,所述反应盘每隔固定时间旋转固定的角度,转送所述反应容器位到所述的孵育转移位或所述的清洗分离转移位。The automatic analyzer according to claim 1, wherein said rotating device is a reaction disk, said reaction disk is rotated at a fixed angle every fixed time, and said reaction container is transferred to said incubation transfer position or The cleaning separates the transfer sites.
- 根据权利要求1所述的自动分析装置,其特征在于,还包括加注站,接收和承载需要加注样本或/和试剂的反应容器。The automatic analyzer according to claim 1, further comprising a filling station for receiving and carrying a reaction container to which a sample or/and a reagent is to be filled.
- 根据权利要求5所述的自动分析装置,其特征在于,所述加注站在所述转移单元的水平运动范围内或可水平运动到所述转移单元的水平运动范围内。The automatic analyzer according to claim 5, wherein said filling station is within a horizontal range of motion of said transfer unit or horizontally movable to a horizontal range of motion of said transfer unit.
- 根据权利要求5所述的自动分析装置,其特征在于:还包括混匀机构,所述混匀机构集成于加注站,用于对反应容器内的反应物混匀。The automatic analyzer according to claim 5, further comprising a mixing mechanism integrated in the filling station for mixing the reactants in the reaction vessel.
- 一种样本分析方法,其特征在于,包括:A sample analysis method, comprising:加注步骤,在反应容器中加注样本和/或试剂;a filling step of filling a sample and/or reagent in the reaction vessel;孵育步骤,对通过至少一个孵育转移位进入反应单元的至少包括需要孵育两次的反应容器进行孵育;An incubation step of incubating at least one reaction vessel that needs to be incubated twice by entering at least one incubation transfer site into the reaction unit;清洗分离步骤,对通过至少一个清洗分离转移位进入反应单元的反应容器进行清洗分离,以去除反应物中未结合的成分;a washing and separating step of washing and separating the reaction vessel entering the reaction unit through at least one washing and separating transfer point to remove unbound components in the reactant;加注信号试剂步骤,向反应容器内加注信号试剂, Filling the signal reagent reagent step, adding a signal reagent to the reaction vessel,测量步骤,通过测量装置对反应容器内的反应信号进行测量。The measuring step measures the reaction signal in the reaction vessel by the measuring device.
- 根据权利要求8所述的样本分析方法,其特征在于:还包括转移步骤,通过转移单元从所述至少一个孵育转移位、至少一个清洗分离转移位将反应容器移入移出所述反应单元。The sample analysis method according to claim 8, further comprising a transferring step of moving the reaction container into and out of the reaction unit by the transfer unit from the at least one incubation transfer position and the at least one wash separation transfer position.
- 根据权利要求8所述的样本分析方法,其特征在于:还包括混匀步骤,对反应容器内的反应物混匀。 The sample analysis method according to claim 8, further comprising the step of mixing to mix the reactants in the reaction vessel.
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