JPH0643163A - Enzyme immunity automatic measurement method - Google Patents
Enzyme immunity automatic measurement methodInfo
- Publication number
- JPH0643163A JPH0643163A JP3039046A JP3904691A JPH0643163A JP H0643163 A JPH0643163 A JP H0643163A JP 3039046 A JP3039046 A JP 3039046A JP 3904691 A JP3904691 A JP 3904691A JP H0643163 A JPH0643163 A JP H0643163A
- Authority
- JP
- Japan
- Prior art keywords
- solid phase
- reaction
- enzyme
- sample
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
【発明の詳細な説明】
【0001】
【産業上の利用分野】本発明は酵素免疫測定方法に関す
るものである。
【0002】
【従来の技術】従来、酵素免疫測定方法においては、標
識物質として酵素を用い、抗原抗体反応によりサンプル
中の特定物質を測定するために、抗原または抗体を固定
化した固相を収容する反応容器にサンプルまたは酵素標
識試薬を分注して固相とサンプルまたは酵素標識試薬と
の反応を行った後に、反応によって結合した物質(Boun
d) と結合していな物質(Free)との分離(B−F分離)
を行い、固相上の酵素活性を測定してサンプル中の抗原
または抗体の測定を行っている。
【0003】
【発明が解決しようとする課題】従来の酵素免疫測定方
法では、B−F分離を行うために固相を反応容器から別
の容器に移しているので、必要とする反応容器の個数が
多くなり、B−F分離を能率良く自動的に行うことがで
きないとともに測定機が大型になり、固相の搬送機構も
複雑となる欠点があった。また、固相とサンプルまたは
酵素標識試薬との反応を効率良く行うことができないた
め、反応時間が長くなり、処理能力が低くなる欠点があ
った。
【0004】本発明は、反応容器に分注したサンプルま
たは酵素標識試薬と固相との反応を促進して分析能力を
向上するとともに抗原抗体反応を行った後のB−F分離
を洗浄装置によって行うことにより酵素免疫測定を自動
的に行うことができるようにした酵素免疫測定方法を提
供することを目的とするものである。
【0005】
【課題を解決するための手段】本発明は、標識物質とし
て酵素を用い、抗原抗体反応によりサンプル中の特定物
質を自動的に測定するに当たり、抗原または抗体を固定
化した固相を収容する反応容器にサンプルまたは酵素標
識試薬を分注した後に、反応液中に空気を吹き込むこと
により反応容器中で進行する固相とサンプルまたは酵素
標識試薬との反応を促進させ、次に反応容器の上部開口
より洗浄液を注入し反応容器内の液体を反応容器外に吸
引排出して固相を反応容器内に残したままで固相および
反応容器を洗浄して非結合物質を除去した後、反応後の
酵素活性を測定することを特徴とするものである。
【0006】
【作用】このような本発明の酵素免疫測定方法において
は、固相を収容した反応容器にサンプルまたは酵素標識
試薬を分注した後、反応液中に空気を吹き込んで攪拌を
行うためこれらの反応を促進することができ、短い反応
時間で十分な反応を行わせることができるとともに固相
を反応容器に入れたままでの洗浄液の注入、吸引排出に
よってB−F分離を行うことができるので、使用する反
応容器の個数を減らすことができる。
【0007】
【実施例】次に、図面につき本発明を詳細に説明する。
図1は酵素免疫反応の概略図である。1は、ポリスチレ
ンボールまたはガラスビーズ等の固相に抗体(あるいは
抗原)2aを固定した固定化固相である(A)。この固
定化固相1に抗体(あるいは抗原)2aに対する抗原
(あるいは抗体)2bを含む血清あるいはこれに相応す
る試料を加えて反応させる(B)。すると、抗原(ある
いは抗体)2bは固定化固相1上の抗体(あるいは抗
原)2aと反応し、抗原(抗体)−抗体(抗原)複合物
固定化固相3を生成する(C)。この複合物固定化固相
3に、固定化固相1に固定化した抗体(あるいは抗原)
2aと同一の抗体(あるいは抗原)に酵素を標識付けし
た酵素標識抗体(あるいは抗原)4を加えて反応させる
(D)。これにより、酵素標識抗体(あるいは抗原)4
と複合物固定化固相3の複合物である酸素標識抗体(抗
原)−抗原(抗体)−抗体(抗原)複合物固定化固相5
を生成する(E)。過剰の酵素標識抗体(あるいは抗
原)4を除去し、前記固定化固相5上の酵素活性を測定
する。これによって試料中の抗原(あるいは抗体)の含
有量を知ることができる。
【0008】本発明においては、固定化固相1を入れた
反応容器に試料あるいは酵素標識試薬を加えて反応液に
空気を吹き込んで抗原抗体反応を行わせて複合物固定化
固相3または5を生成した後、反応容器に洗浄液を供給
し、次いで洗浄液を吸引排出することにより反応容器内
に固相3または5を残したままでB−F分離を行うもの
である。
【0009】図2は本発明の酵素免疫自動測定方法を実
施する自動測定機の一実施例を示す部分図である。大口
部6aおよび小口部6bを備えたU字管6に、大口部6
aから自由に出し入れでき、小口部6bに入らない固定
化固相1を用意する。U字管6の大口部6aより固定化
固相1と試薬あるいは緩衝液を加える(F)。次にサン
プル分注器7により測定対象のサンプルを一定量加え
(G)、小口部6bから空気を吹込み攪拌を行い上述の
固定化固相3を生成させる(H)。この後、大口部6a
に洗浄液供給装置8、小口部6bに洗浄液吸引装置9を
適用させ前記固定化固相3およびU字管6の洗浄を行う
(I)。
【0010】次に上述の酵素標識抗体(あるいは抗原)
4を分注器10により、U字管6の大口部6aより加え
(J)、小口部6bより空気を吹込み攪拌を行い、上述
の固定化固相5を生成させる(K)。この後、洗浄液供
給装置8および洗浄液吸引装置9によって前記固定化固
相5およびU字管6の洗浄を行う(L)。
【0011】次に固定化固相5を含むU字管6内に、固
定化固相5上の酵素活性を測定するための試薬を、試薬
分注器11によって加える(M)。この後小口部6bよ
り空気を吹込み攪拌を行い、反応を行った後(N)、吸
引チューブ12によって反応溶液のみをフローセル13
内に移送する(O)。この反応溶液の吸光度を測定し、
固定化固相5上の酵素活性を求める。これにより、試料
中の抗原(あるいは抗体)2aの含有量を知ることがで
きる。また、吸光度測定後、U字管6内に残った固定化
固相5は大口部6aに設けた固定化固相吸引装置14に
よって除去し、小口部6bに設けた洗浄液供給装置15
により洗浄液をU字管6内に供給し、洗浄後、吸引装置
14により除去する。
【0012】図3は、図2をさらに詳細に説明するた
め、本発明による酵素免疫自動測定方法を実施する自動
測定機の一実施例を示す全体図である。
【0013】図3は、大口部6aおよび小口部6bを具
えた複数のU字管を保持する恒温槽を上から見た図であ
る。恒温槽の上面には反応管を移動させる反応管ターレ
ット16が設けてあり、このターレット16は矢印の方
向に所定ピッチで回動するリング状円板である。一定量
の固相を固定化固相供給装置17から大口部6aに供給
する。
【0014】次に、ターレット16が回動し、サンプル
分注器7によりサンプルを一定量加える。このサンプル
分注器7は、矢印の方向に所定ピッチで回動するサンプ
ラ18のサンプルカップ19からサンプルを、シリンジ
20の上下動およびプローブ21の回動と上下動により
U字管の大口部6aに分注する。このサンプルの分注と
同時に又はその前後において適当な試薬又は緩衝液を分
注するように構成することもできる。22はU字管の小
口部6bに空気を送るエアポンプである。小口部6bか
ら空気を吹込み、固定化固相とサンプルを攪拌し完全に
反応させる。
【0015】次に小口部6bから液排出ポンプ23によ
り、未反応の液を排出する。同時に洗浄用ポンプ24に
より大口部6aから洗浄液を供給し、固相を残したまま
固相とU字管の洗浄を行なってB−F分離を行う。洗浄
液は液排出ポンプ23により排出する。小口部6bの上
面には上下動する円板(図示外)を設け、小口部6bを
介して空気および液を給排する際にこの円板が下降して
給排チューブが小口部6bと連結されるようにし、ター
レット16の回動時には上昇するように配置する。
【0016】次に、酵素標識試薬を分注器10により、
大口部6aから分注する。上に述べたと同様にエアポン
プ22から小口部6bに空気を吹込み攪拌し、液排出ポ
ンプ23により液を排出する。洗浄用ポンプ24により
大口部6aから洗浄液を供給し、固相を残したまま固相
とU字管を洗浄後、洗浄液を液排出ポンプ23により排
出する。
【0017】次に、試薬分注器11によって、大口部6
aから酵素活性を測定するための試薬を加え、エアポン
プ22から小口部6bに空気を吹込み攪拌する。次い
で、小口部6bから減圧ポンプ25により反応溶液をフ
ローセル26に移送する。このフローセル26で吸光度
を測定する。27は光源、28はフィルタ、29は受光
素子である。
【0018】測定後、大口部6aに密閉連結し得る吸引
装置14によりU字管の内容物を排出し、小口部6bに
取付けた洗浄液供給装置15により洗浄液を供給しU字
管を洗浄後、洗浄液を吸引装置14により除去する。
【0019】上記の実施例においてU字管を使用して酵
素免疫反応を行わせたが、本発明は特にU字管に限られ
るものではなく、種々の反応容器が使用可能である。
【0020】
【発明の効果】以上述べたように本発明によれば、反応
容器に固相を収容した状態で、反応容器を変えることな
く、サンプル分析に必要な、全ての反応、固相に未結合
の物質の除去などの一連の操作を実施することが可能と
なり、分析に必要な反応容器の個数を少なくすることが
でき、これにより、酵素免疫自動測定機の小型化、容器
から容器への煩雑な固相の移し変え操作を省くことがで
きる。さらに、本発明では空気を吹き込むことにより固
相とサンプルまたは試薬との反応を促進するので反応時
間が短縮され単位時間当たりの処理能力を向上させるこ
とができる。Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an enzyme immunoassay method. [0002] Conventionally, in the enzyme immunoassay method, an enzyme is used as a labeling substance, and a solid phase on which an antigen or an antibody is immobilized is contained in order to measure a specific substance in a sample by an antigen-antibody reaction. After the sample or enzyme-labeled reagent is dispensed into the reaction vessel and the solid phase reacts with the sample or enzyme-labeled reagent, the substance bound by the reaction (Boun
d) Separation of substances not bound to (Free) (BF separation)
Then, the enzyme activity on the solid phase is measured to measure the antigen or antibody in the sample. In the conventional enzyme immunoassay method, since the solid phase is transferred from the reaction container to another container in order to carry out BF separation, the number of reaction containers required is increased. However, the BF separation cannot be performed efficiently and automatically, the measuring machine becomes large, and the solid phase transfer mechanism becomes complicated. Further, since the reaction between the solid phase and the sample or the enzyme-labeled reagent cannot be performed efficiently, there is a drawback that the reaction time becomes long and the processing ability becomes low. The present invention promotes the reaction between a sample dispensed in a reaction container or an enzyme-labeled reagent and a solid phase to improve the analytical ability, and performs BF separation after carrying out an antigen-antibody reaction by a washing device. It is an object of the present invention to provide an enzyme immunoassay method capable of automatically performing enzyme immunoassay by performing the method. According to the present invention, an enzyme is used as a labeling substance, and when a specific substance in a sample is automatically measured by an antigen-antibody reaction, a solid phase on which an antigen or an antibody is immobilized is used. After the sample or enzyme-labeled reagent is dispensed into the containing reaction vessel, air is blown into the reaction solution to accelerate the reaction between the solid phase that proceeds in the reaction vessel and the sample or enzyme-labeled reagent, and then the reaction vessel After injecting the cleaning liquid from the upper opening of the reactor and sucking and discharging the liquid in the reaction container to the outside of the reaction container to wash the solid phase and the reaction container while leaving the solid phase in the reaction container to remove unbound substances, the reaction It is characterized by measuring the subsequent enzyme activity. In the enzyme immunoassay method of the present invention as described above, after a sample or an enzyme labeling reagent is dispensed into a reaction container containing a solid phase, air is blown into the reaction solution to perform stirring. These reactions can be promoted, a sufficient reaction can be performed in a short reaction time, and BF separation can be performed by injecting a cleaning solution with the solid phase kept in the reaction container and sucking and discharging. Therefore, the number of reaction vessels used can be reduced. The present invention will now be described in detail with reference to the drawings.
FIG. 1 is a schematic diagram of enzyme immunoreaction. Reference numeral 1 is an immobilized solid phase in which an antibody (or antigen) 2a is immobilized on a solid phase such as polystyrene balls or glass beads (A). A serum containing an antigen (or antibody) 2b against the antibody (or antigen) 2a or a sample corresponding thereto is added to the immobilized solid phase 1 and reacted (B). Then, the antigen (or antibody) 2b reacts with the antibody (or antigen) 2a on the immobilized solid phase 1 to generate an antigen (antibody) -antibody (antigen) complex-immobilized solid phase 3 (C). Antibodies (or antigens) immobilized on the solid phase 1 on which the complex is immobilized
An enzyme-labeled antibody (or antigen) 4 obtained by labeling an enzyme with the same antibody (or antigen) as 2a is added and reacted (D). Thus, the enzyme-labeled antibody (or antigen) 4
And an oxygen-labeled antibody (antigen) -antigen (antibody) -antibody (antigen) complex-immobilized solid phase 5 which is a complex of
Is generated (E). Excess enzyme-labeled antibody (or antigen) 4 is removed, and the enzyme activity on the immobilized solid phase 5 is measured. By this, the content of the antigen (or antibody) in the sample can be known. In the present invention, the sample or the enzyme labeling reagent is added to the reaction vessel containing the immobilized solid phase 1 and air is blown into the reaction solution to carry out an antigen-antibody reaction to cause the complex immobilized solid phase 3 or 5 to react. Is generated, the cleaning liquid is supplied to the reaction container, and then the cleaning liquid is sucked and discharged to perform BF separation while leaving the solid phase 3 or 5 in the reaction container. FIG. 2 is a partial view showing an embodiment of an automatic measuring machine for carrying out the enzyme immunoassay automatic measuring method of the present invention. In the U-shaped pipe 6 having the large mouth portion 6a and the small mouth portion 6b, the large mouth portion 6
An immobilized solid phase 1 that can be freely taken in and out from a and does not enter the foremost portion 6b is prepared. The immobilized solid phase 1 and the reagent or buffer solution are added from the large opening 6a of the U-shaped tube 6 (F). Next, a fixed amount of a sample to be measured is added by the sample dispenser 7 (G), and air is blown from the small opening 6b to stir to generate the immobilized solid phase 3 (H). After this, the large portion 6a
The cleaning liquid supply device 8 and the cleaning liquid suction device 9 are applied to the small portion 6b to clean the immobilized solid phase 3 and the U-shaped tube 6 (I). Next, the above-mentioned enzyme-labeled antibody (or antigen)
4 is added from the large opening portion 6a of the U-shaped tube 6 by the dispenser 10 (J), and air is blown from the small opening portion 6b to perform stirring to generate the above-mentioned immobilized solid phase 5 (K). After that, the immobilized solid phase 5 and the U-shaped tube 6 are cleaned by the cleaning liquid supply device 8 and the cleaning liquid suction device 9 (L). Next, a reagent for measuring the enzyme activity on the immobilized solid phase 5 is added into the U-shaped tube 6 containing the immobilized solid phase 5 by a reagent dispenser 11 (M). After that, air is blown from the small portion 6b to perform stirring to carry out the reaction (N), and then only the reaction solution is flown by the suction tube 12 to the flow cell 13
Transfer inside (O). Measure the absorbance of this reaction solution,
The enzyme activity on the immobilized solid phase 5 is determined. Thereby, the content of the antigen (or antibody) 2a in the sample can be known. Further, after the measurement of the absorbance, the immobilized solid phase 5 remaining in the U-shaped tube 6 is removed by the immobilized solid phase suction device 14 provided in the large mouth portion 6a, and the cleaning liquid supply device 15 provided in the small mouth portion 6b.
The cleaning liquid is supplied into the U-shaped tube 6 by means of, and after cleaning, it is removed by the suction device 14. FIG. 3 is a general view showing an embodiment of an automatic measuring machine for carrying out the enzyme-linked immunosorbent automatic measuring method according to the present invention in order to explain FIG. 2 in more detail. FIG. 3 is a view from above of a thermostat for holding a plurality of U-shaped tubes having a large mouth portion 6a and a small mouth portion 6b. A reaction tube turret 16 for moving the reaction tube is provided on the upper surface of the constant temperature bath, and the turret 16 is a ring-shaped disc that rotates at a predetermined pitch in the direction of the arrow. A fixed amount of solid phase is supplied from the immobilized solid phase supply device 17 to the large mouth portion 6a. Next, the turret 16 is rotated and the sample dispenser 7 adds a fixed amount of sample. The sample dispenser 7 samples a sample from a sample cup 19 of a sampler 18 that rotates at a predetermined pitch in the direction of an arrow by vertically moving a syringe 20 and rotating and vertically moving a probe 21 so that a large mouth portion 6a of a U-shaped tube is formed. Dispense into. A suitable reagent or buffer may be dispensed at the same time as or before or after the dispensing of the sample. An air pump 22 sends air to the small opening 6b of the U-shaped tube. Air is blown from the small opening 6b to stir the immobilized solid phase and the sample to completely react. Next, the unreacted liquid is discharged from the small portion 6b by the liquid discharge pump 23. At the same time, a cleaning liquid is supplied from the large opening 6a by the cleaning pump 24, and the solid phase and the U-shaped tube are cleaned while leaving the solid phase, and BF separation is performed. The cleaning liquid is discharged by the liquid discharge pump 23. A disc (not shown) that moves up and down is provided on the upper surface of the small portion 6b, and when supplying and discharging air and liquid through the small portion 6b, the circular plate descends to connect the supply / discharge tube to the small portion 6b. And is arranged so as to rise when the turret 16 rotates. Next, the enzyme labeling reagent is dispensed by the dispenser 10.
Dispense from the large portion 6a. In the same manner as described above, air is blown from the air pump 22 into the small opening 6b to stir, and the liquid is discharged by the liquid discharge pump 23. The cleaning liquid is supplied from the large opening 6a by the cleaning pump 24, the solid phase and the U-shaped tube are cleaned while leaving the solid phase, and then the cleaning liquid is discharged by the liquid discharge pump 23. Next, the reagent dispenser 11 is used to
A reagent for measuring the enzyme activity is added from a, and air is blown from the air pump 22 into the small portion 6b and stirred. Then, the reaction solution is transferred to the flow cell 26 by the decompression pump 25 from the small portion 6b. The absorbance is measured by this flow cell 26. 27 is a light source, 28 is a filter, and 29 is a light receiving element. After the measurement, the contents of the U-shaped pipe are discharged by the suction device 14 which can be hermetically connected to the large mouth portion 6a, and the cleaning liquid is supplied by the cleaning liquid supply device 15 attached to the small mouth portion 6b to wash the U-shaped pipe. The cleaning liquid is removed by the suction device 14. Although the U-shaped tube is used for the enzyme immunoreaction in the above-mentioned embodiment, the present invention is not limited to the U-shaped tube and various reaction vessels can be used. As described above, according to the present invention, in a state where the solid phase is contained in the reaction container, all the reactions and solid phases necessary for sample analysis can be obtained without changing the reaction container. It is possible to carry out a series of operations such as removal of unbound substances, and it is possible to reduce the number of reaction vessels required for analysis. This makes it possible to reduce the size of the enzyme immunoassay analyzer and from vessel to vessel. It is possible to omit the complicated solid phase transfer operation. Furthermore, in the present invention, the reaction between the solid phase and the sample or reagent is promoted by blowing air, so that the reaction time is shortened and the processing capacity per unit time can be improved.
【図面の簡単な説明】
【図1】酵素免疫反応の概略図である。
【図2】本発明による酵素免疫自動測定方法における順
次の工程を示す図である。
【図3】本発明の酵素免疫自動測定方法を実施する装置
の一実施例の構成を示す図である。
【符号の説明】
1 固定化固相
2 抗原(抗体)
3 抗原─抗体複合物固定化固相
4 酵素標識抗体
5 酵素標識抗体─抗原─抗体複合物固定化固相
6 U字管
7 試料分注器
8 洗浄水供給装置
9 洗浄水吸引装置
10 分注器
11 試薬分注器
12 吸引チューブ
13 フローセル
14 固定化固相吸引装置
15 小口部用洗浄水供給装置
16 反応管ターレット
22 エアポンプ
23 液排出ポンプ
24 洗浄用ポンプBRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram of enzyme immunoreaction. FIG. 2 is a diagram showing sequential steps in the enzyme-immunoassay automatic measuring method according to the present invention. FIG. 3 is a diagram showing the configuration of an embodiment of an apparatus for carrying out the enzyme-immunoassay automatic measuring method of the present invention. [Explanation of symbols] 1 immobilized solid phase 2 antigen (antibody) 3 antigen-antibody complex immobilized solid phase 4 enzyme labeled antibody 5 enzyme labeled antibody-antigen-antibody complex immobilized solid phase 6 U-tube 7 sample Injection device 8 Wash water supply device 9 Wash water suction device 10 Dispenser 11 Reagent dispenser 12 Suction tube 13 Flow cell 14 Immobilized solid phase suction device 15 Wash water supply device for small mouth portion 16 Reaction tube turret 22 Air pump 23 Liquid discharge Pump 24 Cleaning pump
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 FI 技術表示箇所 G01N 35/06 A 8310−2J ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification number Office reference number FI technical display location G01N 35/06 A 8310-2J
Claims (1)
ンプル中の特定物質を自動的に測定するに当たり、抗原
または抗体を固定化した固相を収容する反応容器にサン
プルまたは酵素標識試薬を分注した後に、反応液中に空
気を吹き込むことにより反応容器中で進行する固相とサ
ンプルまたは酵素標識試薬との反応を促進させ、次に反
応容器の上部開口より洗浄液を注入し反応容器内の液体
を反応容器外に吸引排出して固相を反応容器内に残した
ままで固相および反応容器を洗浄して非結合物質を除去
した後、反応後の酵素活性を測定することを特徴とする
酵素免疫測定方法。[Claims] 1. When an enzyme is used as a labeling substance and a specific substance in a sample is automatically measured by an antigen-antibody reaction, the sample or the enzyme is placed in a reaction container containing a solid phase on which an antigen or antibody is immobilized. After the labeling reagent is dispensed, air is blown into the reaction solution to accelerate the reaction between the solid phase and the sample or enzyme labeling reagent that proceed in the reaction vessel, and then the washing solution is injected from the upper opening of the reaction vessel. To measure the enzyme activity after the reaction after removing the non-binding substances by washing the solid phase and the reaction container with the solid phase remaining inside the reaction container by suctioning and discharging the liquid in the reaction container outside the reaction container. An enzyme immunoassay method characterized by:
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3039046A JPH0643163A (en) | 1991-02-12 | 1991-02-12 | Enzyme immunity automatic measurement method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3039046A JPH0643163A (en) | 1991-02-12 | 1991-02-12 | Enzyme immunity automatic measurement method |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6107387A Division JPS6324161A (en) | 1987-03-18 | 1987-03-18 | Automatic enzyme immunity measurement method |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH0643163A true JPH0643163A (en) | 1994-02-18 |
Family
ID=12542185
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3039046A Pending JPH0643163A (en) | 1991-02-12 | 1991-02-12 | Enzyme immunity automatic measurement method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0643163A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001027619A1 (en) * | 1999-10-14 | 2001-04-19 | Central Research Institute Of Electric Power Industry | Method for detecting exogenous endocrine disrupting chemical and detection apparatus |
JP2003227841A (en) * | 2002-02-01 | 2003-08-15 | Fuji Photo Film Co Ltd | Method for association reaction between receptor and ligand and unit for biochemical analysis used for the same |
US20200299672A1 (en) * | 2019-03-18 | 2020-09-24 | Cellular Research, Inc. | Precise delivery of components into fluids |
-
1991
- 1991-02-12 JP JP3039046A patent/JPH0643163A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001027619A1 (en) * | 1999-10-14 | 2001-04-19 | Central Research Institute Of Electric Power Industry | Method for detecting exogenous endocrine disrupting chemical and detection apparatus |
JP2003227841A (en) * | 2002-02-01 | 2003-08-15 | Fuji Photo Film Co Ltd | Method for association reaction between receptor and ligand and unit for biochemical analysis used for the same |
US20200299672A1 (en) * | 2019-03-18 | 2020-09-24 | Cellular Research, Inc. | Precise delivery of components into fluids |
US11976269B2 (en) * | 2019-03-18 | 2024-05-07 | Cellular Research, Inc. | Precise delivery of components into fluids |
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