JPH0118383B2 - - Google Patents
Info
- Publication number
- JPH0118383B2 JPH0118383B2 JP55049140A JP4914080A JPH0118383B2 JP H0118383 B2 JPH0118383 B2 JP H0118383B2 JP 55049140 A JP55049140 A JP 55049140A JP 4914080 A JP4914080 A JP 4914080A JP H0118383 B2 JPH0118383 B2 JP H0118383B2
- Authority
- JP
- Japan
- Prior art keywords
- solid phase
- antigen
- enzyme
- antibody
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000007790 solid phase Substances 0.000 claims description 48
- 238000006243 chemical reaction Methods 0.000 claims description 40
- 102000004190 Enzymes Human genes 0.000 claims description 34
- 108090000790 Enzymes Proteins 0.000 claims description 34
- 239000000427 antigen Substances 0.000 claims description 25
- 102000036639 antigens Human genes 0.000 claims description 23
- 108091007433 antigens Proteins 0.000 claims description 23
- 238000004140 cleaning Methods 0.000 claims description 20
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 12
- 238000003018 immunoassay Methods 0.000 claims description 8
- 238000005259 measurement Methods 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 6
- 238000002372 labelling Methods 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 3
- 239000012071 phase Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 description 22
- 239000000523 sample Substances 0.000 description 17
- 238000010586 diagram Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000003028 enzyme activity measurement method Methods 0.000 description 3
- 230000036046 immunoreaction Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 1
- 238000011481 absorbance measurement Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
Description
【発明の詳細な説明】
本発明は自動的に酵素免疫を測定する測定機に
関するものであり、特に、反応によつて結合した
物質（Bound）と結合していない物質（Free）
の分離（Ｂ−Ｆ分離）が可能な酵素免疫自動測定
機に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a measuring device that automatically measures enzyme immunity, and in particular, a substance bound by a reaction (Bound) and a substance not bound (Free).
This invention relates to an automatic enzyme immunoassay device capable of separating (B-F separation).
従来、酵素免疫測定は複雑な測定法であり、最
終段階の酵素活性測定は簡単に自動測定を行うこ
とができるにもかかわらず、Ｂ−Ｆ分離を行う操
作段階において、Ｂ−Ｆ分離が容易でない所から
全過程を自動化することが困難であつた。また、
生化学自動分析機は通常測定終了後の洗浄装置を
装備しているが、分析途中で洗浄するような装置
を備えたものは殆どない。従つて酵素免疫測定に
おいても、分析途中の洗浄は用手法で行わなけれ
ばならなかつた。 Conventionally, enzyme immunoassay is a complicated measurement method, and although the enzyme activity measurement at the final stage can be easily performed automatically, it is difficult to separate B-F at the operational stage where B-F separation is performed. It was difficult to automate the entire process from a place where there was no such thing. Also,
Automatic biochemical analyzers are usually equipped with a cleaning device after the end of a measurement, but very few are equipped with a device that cleans the analyzer mid-way through analysis. Therefore, even in enzyme immunoassays, cleaning during the analysis had to be performed manually.
本発明は、酵素免疫測定において分析途中のＢ
−Ｆ分離を洗浄により自動的に行うことができる
ともに処理能率を上げ、ランニングコストを低く
するために反応容器を繰り返し使用することがで
きるようにした酵素免疫自動測定機を提供するこ
とを目的とするものである。 The present invention provides B in the middle of analysis in enzyme immunoassay.
-The purpose of the present invention is to provide an automatic enzyme immunoassay device that can automatically perform -F separation by washing, and also allows the reaction vessel to be used repeatedly in order to increase processing efficiency and reduce running costs. It is something to do.
本発明の酵素免疫自動測定機は、標識物質とし
て酵素を用い、抗原抗体反応によりサンプル中の
特定物質を測定するに当たり、反応容器を反応ラ
インに沿つて搬送する手段と、抗原または抗体を
固定化した固相を反応容器に供給する固相供給手
段と、反応容器にサンプルを分注する手段と、反
応容器に酵素標識試薬を分注する手段と、抗原抗
体反応を行つた後に固相を反応容器内に残したま
まで固相および反応容器を洗浄することによつて
Ｂ−Ｆ分離を自動的に行う洗浄手段と、抗原抗体
反応により形成される複合物固定化固相の酵素活
性を測定する手段と、測定が終了した反応容器か
ら複合物固定化固相を排出して再使用に備える手
段とを具えたものである。 The automatic enzyme immunoassay device of the present invention uses an enzyme as a labeling substance and measures a specific substance in a sample through an antigen-antibody reaction. A solid phase supply means for supplying the solid phase to the reaction vessel, a means for dispensing the sample into the reaction vessel, a means for dispensing an enzyme labeled reagent to the reaction vessel, and a means for dispensing the solid phase to the reaction vessel after performing the antigen-antibody reaction. A cleaning means that automatically performs B-F separation by washing the solid phase and the reaction vessel while leaving it in the container, and measuring the enzyme activity of the complex-immobilized solid phase formed by antigen-antibody reaction. and a means for discharging the composite-immobilized solid phase from the reaction vessel after measurement and preparing it for reuse.
次に、図面につき本発明実施例を詳細に説明す
る。 Next, embodiments of the present invention will be described in detail with reference to the drawings.
第１図は酵素免疫反応の概略図である。１は、
ポリスチレンボールまたはガラスビーズ等の固相
に抗体（あるいは抗原）２ａを固定した固定化固
相である(A)。この固定化固相１に抗体（あるいは
抗原）２ａに対する抗原（あるいは抗体）２ｂを
含む血清あるいはこれに相応する試料を加えて反
応させる(B)。すると、抗原（あるいは抗体）２ｂ
は固定化固相１上の抗体（あるいは抗原）２ａと
反応し、抗原（抗体）−抗体（抗原）複合物固定
化固相３を生成する(C)。この複合物固定化固相３
に、固定化固相１に固定化した抗体（あるいは抗
原）２ａと同一の抗体（あるいは抗原）に酵素を
標識付けした酵素標識抗体（あるいは抗原）４を
加えて反応させる(D)。これにより、酵素標識抗体
（あるいは抗原）４と複合物固定化固相３の複合
物である酵素標識抗体（抗原）−抗原（抗体）−抗
体（抗原）複合物固定化固相５を生成する(E)。過
剰の酵素標識抗体（あるいは抗原）４を除去し、
前記固定化固相５上の酵素活性を測定する。これ
によつて試料中の抗原（あるいは抗体）の含有量
を知ることができる。 FIG. 1 is a schematic diagram of the enzyme immunoreaction. 1 is
This is an immobilized solid phase in which antibody (or antigen) 2a is immobilized on a solid phase such as polystyrene balls or glass beads (A). Serum containing the antigen (or antibody) 2b against the antibody (or antigen) 2a or a sample corresponding thereto is added to the immobilized solid phase 1 and reacted (B). Then, antigen (or antibody) 2b
reacts with the antibody (or antigen) 2a on the immobilized solid phase 1 to produce an antigen (antibody)-antibody (antigen) complex immobilized solid phase 3 (C). This composite immobilized solid phase 3
Then, an enzyme-labeled antibody (or antigen) 4, which is an enzyme-labeled antibody (or antigen) identical to the antibody (or antigen) 2a immobilized on the immobilized solid phase 1, is added and reacted (D). As a result, an enzyme-labeled antibody (antigen)-antigen (antibody)-antibody (antigen) complex-immobilized solid phase 5, which is a composite of the enzyme-labeled antibody (or antigen) 4 and the complex-immobilized solid phase 3, is generated. (E). Remove excess enzyme-labeled antibody (or antigen) 4,
The enzyme activity on the immobilized solid phase 5 is measured. This allows the content of antigen (or antibody) in the sample to be determined.
本発明においては、反応管を反応ラインに沿つ
て順次搬送させる手段と、反応管に固定化固相を
供給する手段と、試料、酵素標識試薬を反応管に
分注する手段と、上述の抗原（抗体）−抗体（抗
原）複合物固定化固相３および酵素標識抗体（抗
原）−抗原（抗体）−抗体（抗原）複合物固定化固
相５を生成後、その反応管を洗浄してＢ−Ｆ分離
を自動的に行う手段と、複合物固定化固相の酵素
活性を測定する手段と、測定後、固相を反応管か
ら排出して再使用に備える手段とを具え、酵素免
疫反応を効率良く自動的に測定することができる
ようにした。 In the present invention, there are provided means for sequentially transporting reaction tubes along a reaction line, means for supplying an immobilized solid phase to the reaction tubes, means for dispensing samples and enzyme-labeled reagents into the reaction tubes, and means for dispensing the above-mentioned antigen. After producing the (antibody)-antibody (antigen) complex-immobilized solid phase 3 and the enzyme-labeled antibody (antigen)-antigen (antibody)-antibody (antigen) complex-immobilized solid phase 5, the reaction tube is washed. It is equipped with a means for automatically performing B-F separation, a means for measuring the enzyme activity of the solid phase immobilized with the complex, and a means for discharging the solid phase from the reaction tube after the measurement to prepare for reuse. This makes it possible to efficiently and automatically measure reactions.
第２図はこの自動測定機の一実施例を示す部分
図である。 FIG. 2 is a partial diagram showing one embodiment of this automatic measuring machine.
大口部６ａおよび小口部６ｂを備えたＵ字管６
に、大口部６ａから自由に出し入れでき、小口部
６ｂに入らない固定化固相１を用意する。Ｕ字管
６の大口部６ａより固定化固相１と試薬あるいは
緩衝液を加える(F)。次にサンプル分注器７により
測定対象のサンプルを一定量加え(G)、小口部６ｂ
から空気を吹込み撹拌を行い上述の固定化固相３
生成させる(H)。この後、大口部６ａに洗浄液供給
装置８、小口部６ｂに洗浄液吸引装置９を適用さ
せ前記固定化固相３およびＵ字管６の洗浄を行う
(I)。次に上述の酵素標識抗体（あるいは抗原）４
を分注器１０により、Ｕ字管６の大口部６ａより
加え(J)、小口部６ｂより空気を吹込み撹拌を行
い、上述の固定化固相５を生成させる(K)。この
後、洗浄液供給装置８および洗浄液吸引装置９に
よつて、前記固定化固相５およびＵ字管６の洗浄
を行う(L)。次に固定化固相５を含むＵ字管６内
に、固定化固相５上の酵素活性を測定するための
試薬を、試薬分注器１１によつて加える（Ｍ）。
この後小口部６ｂより空気を吹込み撹拌を行い、
反応を行つた後（Ｎ）、吸引チユーブ１２によつ
て反応溶液をフローセル１３内に移送する（Ｏ）。
この反応溶液の吸光度を測定し、固定化固相５上
の酵素活性を求める。これにより、試料中の抗原
（あるいは抗体）２ａの含有量を知ることができ
る。また、吸光度測定後、Ｕ字管６内に残つた固
定化固相５は大口部６ａに設けた固定化固相吸引
装置１４によつて除去し、小口部６ｂに設けた洗
浄液供給装置１５により洗浄液をＵ字管６内に供
給し、洗浄後、吸引装置１４により除去し、次の
測定に備えるようにする。 U-shaped tube 6 with a large opening 6a and a small opening 6b
First, an immobilized solid phase 1 is prepared which can be freely taken in and taken out from the large opening 6a and which does not enter the small opening 6b. The immobilized solid phase 1 and reagent or buffer are added through the large opening 6a of the U-shaped tube 6 (F). Next, add a certain amount of the sample to be measured using the sample dispenser 7 (G), and
Air is blown in and stirred to form the above-mentioned immobilized solid phase 3.
Generate (H). Thereafter, the immobilized solid phase 3 and the U-shaped tube 6 are washed by applying a washing liquid supply device 8 to the large opening 6a and a washing liquid suction device 9 to the small opening 6b.
(I). Next, the above-mentioned enzyme-labeled antibody (or antigen) 4
is added from the large opening 6a of the U-shaped tube 6 using the dispenser 10 (J), and air is blown into the small opening 6b for stirring to produce the above-mentioned immobilized solid phase 5 (K). Thereafter, the immobilized solid phase 5 and the U-shaped tube 6 are washed using the washing liquid supply device 8 and the washing liquid suction device 9 (L). Next, a reagent for measuring the enzyme activity on the immobilized solid phase 5 is added into the U-shaped tube 6 containing the immobilized solid phase 5 using the reagent dispenser 11 (M).
After that, air is blown in from the small opening 6b to stir the mixture.
After the reaction (N), the reaction solution is transferred into the flow cell 13 by the suction tube 12 (O).
The absorbance of this reaction solution is measured to determine the enzyme activity on the immobilized solid phase 5. This makes it possible to know the content of antigen (or antibody) 2a in the sample. Further, after the absorbance measurement, the immobilized solid phase 5 remaining in the U-shaped tube 6 is removed by the immobilized solid phase suction device 14 provided at the large opening 6a, and is removed by the cleaning liquid supply device 15 provided at the small opening 6b. A cleaning liquid is supplied into the U-shaped tube 6, and after cleaning, it is removed by the suction device 14 in preparation for the next measurement.
第３図は、第２図をさらに詳細に説明するた
め、本発明による自動測定機の一実施例を示す全
体図である。 FIG. 3 is an overall view showing an embodiment of the automatic measuring machine according to the present invention, in order to explain FIG. 2 in more detail.
第３図は、大口部６ａおよび小口部６ｂを備え
た複数のＵ字管を保持する恒温槽を上から見た図
である。恒温槽の上面には反応管を移動させる反
応管ターレツト１６が設けてあり、このターレツ
ト１６は矢印の方向に所定ピツチで回動するリン
グ状円板である。一定量の固相を固定化固相供給
装置１７から大口部６ａに供給する。次に、ター
レツト１６が回動し、サンプル分注器７によりサ
ンプルを一定量加える。このサンプル分注器７
は、矢印の方向に所定ピツチで回動するサンプラ
１８のサンプルカツプ１９からサンプルを、シリ
ンジ２０の上下動およびプローブ２１の回動と上
下動によりＵ字管の大口部６ａに分注する。この
サンプルの分注と同時に又はその前後において適
当な試薬又は緩衝液を分注するように構成するこ
ともできる。２２はＵ字管の小口部６ｂに空気を
送るエアポンプである。小口部６ｂから空気を吹
込み、固定化固相とサンプルを撹拌し完全に反応
させる。次に小口部６ｂから液排出ポンプ２３に
より、未反応の液を排出する。同時に洗浄用ポン
プ２４により大口部６ａから洗浄液を供給し、固
相とＵ字管の洗浄を行う。洗浄液は液排出ポンプ
２３により排出する。小口部６ｂの上面には上下
動する円板（図示外）を設け、小口部６ｂを介し
て空気および液を給排する際にこの円板が下降し
て給排チユーブが小口部６ｂと連結されるように
し、ターレツト１６の回動時には上昇するように
配置する。 FIG. 3 is a top view of a thermostatic chamber holding a plurality of U-shaped tubes each having a large opening 6a and a small opening 6b. A reaction tube turret 16 for moving the reaction tubes is provided on the upper surface of the thermostatic chamber, and this turret 16 is a ring-shaped disk that rotates at a predetermined pitch in the direction of the arrow. A fixed amount of solid phase is supplied from the immobilized solid phase supply device 17 to the large mouth portion 6a. Next, the turret 16 is rotated and a fixed amount of sample is added by the sample dispenser 7. This sample dispenser 7
The sample is dispensed from the sample cup 19 of the sampler 18, which rotates at a predetermined pitch in the direction of the arrow, into the large opening 6a of the U-shaped tube by the vertical movement of the syringe 20 and the rotation and vertical movement of the probe 21. A suitable reagent or buffer may be dispensed simultaneously with or before or after dispensing the sample. 22 is an air pump that sends air to the small opening 6b of the U-shaped tube. Air is blown from the small opening 6b to stir the immobilized solid phase and the sample to cause a complete reaction. Next, the unreacted liquid is discharged from the small opening 6b by the liquid discharge pump 23. At the same time, the cleaning pump 24 supplies a cleaning liquid from the large opening 6a to clean the solid phase and the U-shaped tube. The cleaning liquid is discharged by a liquid discharge pump 23. A vertically movable disc (not shown) is provided on the upper surface of the mouth part 6b, and when air and liquid are supplied and discharged through the mouth part 6b, this disc descends and the supply/discharge tube is connected to the mouth part 6b. The turret 16 is arranged so that it rises when it rotates.
次に、酵素標識試薬を分注器１０により、大口
部６ａから分注する。上に述べたと同様にエアポ
ンプ２２から小口部６ｂに空気を吹込み撹拌し、
液排出ポンプ２３により液を排出する。洗浄用ポ
ンプ２４により大口部６ａから洗浄液を供給し、
固相とＵ字管を洗浄後、洗浄液を液排出ポンプ２
３により排出する。 Next, the enzyme labeling reagent is dispensed from the large mouth portion 6a using the dispenser 10. Air is blown into the mouth part 6b from the air pump 22 and stirred in the same manner as described above,
The liquid is discharged by the liquid discharge pump 23. Supplying cleaning liquid from the large mouth part 6a by the cleaning pump 24,
After washing the solid phase and U-shaped tube, the washing liquid is sent to the liquid discharge pump 2.
Discharge according to 3.
次に、試薬分注器１１によつて、大口部６ａか
ら酵素活性測定用試薬を加え、エアポンプ２２か
ら小口部６ｂに空気を吹込み撹拌する。次いで、
小口部６ｂから減圧ポンプ２５により反応溶液を
フローセル２６に移送する。このフローセル２６
で吸光度を測定する。２７は光源、２８はフイル
タ、２９は受光素子である。 Next, a reagent for enzyme activity measurement is added from the large mouth part 6a using the reagent dispenser 11, and air is blown into the small mouth part 6b from the air pump 22 for stirring. Then,
The reaction solution is transferred from the small opening 6b to the flow cell 26 by the vacuum pump 25. This flow cell 26
Measure the absorbance. 27 is a light source, 28 is a filter, and 29 is a light receiving element.
測定後、大口部６ａに密閉連結し得る吸引装置
１４によりＵ字管の内容物を排出し、小口部６ｂ
に取付けた洗浄液供給装置１５により洗浄液を供
給しＵ字管を洗浄後、洗浄液を吸引装置１４によ
り除去する。このようにしてＵ字管を再使用する
ことができる。 After the measurement, the contents of the U-shaped tube are discharged by a suction device 14 that can be tightly connected to the large opening 6a, and the contents of the U-shaped tube are removed from the small opening 6b.
After cleaning the U-shaped tube by supplying the cleaning liquid by the cleaning liquid supply device 15 attached to the holder, the cleaning liquid is removed by the suction device 14. In this way the U-tube can be reused.
上記実施例においてＵ字管を使用して酵素免疫
反応を行わせたが、Ｕ字管に限られるものではな
く、固相がＢ−Ｆ分離のための洗浄時の洗浄液の
除去によつて反応容器から吸引排出されないよう
な形状、例えば濾斗状、管内に網状のしきり部を
設けたもの等を使用することができる。 In the above examples, the enzyme immunoreaction was carried out using a U-shaped tube, but the U-shaped tube is not limited to the U-shaped tube. It is possible to use a shape that prevents the liquid from being suctioned and discharged from the container, such as a funnel shape or a tube with a mesh-like partition provided inside the tube.
以上述べたように本発明の酵素免疫自動測定機
は、分析途中で、固相を反応容器内に残したまま
で洗浄を行つてＢ−Ｆ分離を行うことができる洗
浄装置を設けたので、容易に反応物と未反応物の
分離を行うことができ、全過程を自動的に操作す
ることができる。また、この洗浄装置は、簡単な
構造なので、サンプル分注から最終的な比色定量
その他の光学的測定までの反応段階において、任
意の位置に選択的に設置することができ、且つ脱
着可能である。 As described above, the automatic enzyme immunoassay system of the present invention is equipped with a cleaning device that can perform B-F separation by cleaning the solid phase while leaving it in the reaction vessel during the analysis. It is possible to separate reactants and unreacted products, and the entire process can be operated automatically. Furthermore, since this cleaning device has a simple structure, it can be selectively installed at any position during the reaction steps from sample dispensing to final colorimetric determination and other optical measurements, and it is also removable. be.
また、反応容器に固相を供給する手段と、酵素
活性の測定後、反応容器に残つている固相を除去
する手段を設けることによつて反応容器を繰り返
し再使用できるようにしたため、サンプルの連続
的な測定が可能となり、処理能力の向上とランニ
ングコストの軽減を図ることができる。 In addition, by providing a means for supplying a solid phase to the reaction vessel and a means for removing the solid phase remaining in the reaction vessel after enzyme activity measurement, the reaction vessel can be reused repeatedly. Continuous measurement becomes possible, improving processing capacity and reducing running costs.
第１図は酵素免疫反応の概略図、第２図は本発
明による自動測定機における順次の工程を示す線
図、第３図は第２図に示す自動測定機の構成をさ
らに詳細に示した図である。
１……固定化固相、２……抗原（抗体）、３…
…抗原−抗体複合物固定化固相、４……酵素標識
抗体、５……酵素標識抗体−抗原−抗体複合物固
定化固相、６……Ｕ字管、７……試料分注器、８
……洗浄水供給装置、９……洗浄水吸引装置、１
０……分注器、１１……試薬分注器、１２……吸
引チユーブ、１３……フローセル、１４……固定
化固相吸引装置、１５……小口部用洗浄水供給装
置、１６……反応管ターレツト、２２……エアポ
ンプ、２３……液排出ポンプ、２４……洗浄用ポ
ンプ。
FIG. 1 is a schematic diagram of the enzyme immunoreaction, FIG. 2 is a diagram showing the sequential steps in the automatic measuring device according to the present invention, and FIG. 3 shows the configuration of the automatic measuring device shown in FIG. 2 in more detail. It is a diagram. 1...immobilized solid phase, 2...antigen (antibody), 3...
...Antigen-antibody complex immobilized solid phase, 4...Enzyme-labeled antibody, 5...Enzyme-labeled antibody-antigen-antibody complex immobilized solid phase, 6...U-shaped tube, 7...Sample dispenser, 8
...Washing water supply device, 9...Washing water suction device, 1
0... Dispenser, 11... Reagent dispenser, 12... Suction tube, 13... Flow cell, 14... Immobilized solid phase suction device, 15... Small mouth washing water supply device, 16... Reaction tube turret, 22...air pump, 23...liquid discharge pump, 24...cleaning pump.
Claims (1)
よりサンプル中の特定物質を測定するに当たり、 反応容器を反応ラインに沿つて搬送する手段
と、 抗原または抗体を固定化した固相を反応容器に
供給する固相供給手段と、 反応容器にサンプルを分注する手段と、 反応容器に酵素標識試薬または酵素活性測定用
試薬を分注する手段と、 抗原抗体反応を行つた後に固相を反応容器内に
残したままで固相および反応容器を洗浄すること
によつてＢ−Ｆ分離を自動的に行う洗浄手段と、 抗原抗体反応により形成される複合物固定化固
相の酵素活性を測定する手段と、 測定が終了した反応容器から複合物固定化固相
を排出して再使用に備える手段とを具えた酵素免
疫自動測定機。[Claims] 1. When measuring a specific substance in a sample by antigen-antibody reaction using an enzyme as a labeling substance, means for transporting a reaction container along a reaction line, and a solid phase on which an antigen or antibody is immobilized. a means for dispensing a sample into a reaction vessel; a means for dispensing an enzyme labeling reagent or a reagent for measuring enzyme activity into the reaction vessel; A cleaning means that automatically performs B-F separation by washing the solid phase and the reaction vessel while leaving the phase in the reaction vessel, and an enzyme activity of the complex-immobilized solid phase formed by the antigen-antibody reaction. An automatic enzyme immunoassay device, comprising: a means for measuring , and a means for discharging a compound-immobilized solid phase from a reaction vessel after measurement to prepare it for reuse.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4914080A JPS56147067A (en) | 1980-04-16 | 1980-04-16 | Automatic measuring instrument for enzyme immunity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4914080A JPS56147067A (en) | 1980-04-16 | 1980-04-16 | Automatic measuring instrument for enzyme immunity |
Related Child Applications (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6107387A Division JPS6324161A (en) | 1987-03-18 | 1987-03-18 | Automatic enzyme immunity measurement method |
| JP6106087A Division JPS6324159A (en) | 1987-03-18 | 1987-03-18 | Automatic enzyme immunity measuring machine |
| JP6107287A Division JPS6324160A (en) | 1987-03-18 | 1987-03-18 | Automatic enzyme immunity measurement method |
| JP6107487A Division JPS6324162A (en) | 1987-03-18 | 1987-03-18 | Automatic enzyme immunity measurement method |
| JP6105887A Division JPS6324157A (en) | 1987-03-18 | 1987-03-18 | Automatic enzyme immunity measuring machine |
| JP6105987A Division JPS6324158A (en) | 1987-03-18 | 1987-03-18 | Automatic enzyme immunity measuring machine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56147067A JPS56147067A (en) | 1981-11-14 |
| JPH0118383B2 true JPH0118383B2 (en) | 1989-04-05 |
Family
ID=12822767
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4914080A Granted JPS56147067A (en) | 1980-04-16 | 1980-04-16 | Automatic measuring instrument for enzyme immunity |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56147067A (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58219456A (en) * | 1982-06-16 | 1983-12-20 | Aloka Co Ltd | Biochemical assay equipment |
| US5175086A (en) * | 1983-01-24 | 1992-12-29 | Olympus Optical Co., Ltd. | Method for effecting heterogeneous immunological analysis |
| JPS59193359A (en) * | 1983-04-18 | 1984-11-01 | Olympus Optical Co Ltd | Immunological automatic analytical apparatus |
| JPS59135367A (en) * | 1983-01-24 | 1984-08-03 | Olympus Optical Co Ltd | Immunological automatic analytical method |
| JPH0614048B2 (en) * | 1983-02-14 | 1994-02-23 | オリンパス光学工業株式会社 | Enzyme immunological automatic analyzer |
| JPS59188560A (en) * | 1983-04-12 | 1984-10-25 | Olympus Optical Co Ltd | Reaction tube |
| DE3412886A1 (en) * | 1983-04-07 | 1984-10-11 | Olympus Optical Co., Ltd., Tokio/Tokyo | Carrier and reaction tube for immunological analyses |
| JPS60111961A (en) * | 1983-11-22 | 1985-06-18 | Olympus Optical Co Ltd | Method of washing solid phase carrier |
| US4681742A (en) * | 1984-10-01 | 1987-07-21 | Cetus Corporation | Assay tray |
| JPS61161456A (en) * | 1985-01-10 | 1986-07-22 | Sumitomo Electric Ind Ltd | Antibody genetic power detector |
| US4753775A (en) * | 1985-04-12 | 1988-06-28 | E. I. Du Pont De Nemours And Company | Rapid assay processor |
| JP2520136B2 (en) * | 1987-08-27 | 1996-07-31 | オリンパス光学工業株式会社 | Automatic analyzer |
| JP2828143B2 (en) * | 1987-09-28 | 1998-11-25 | オリンパス光学工業株式会社 | Automatic analyzer with an analysis unit based on the immunological analysis principle |
| US9597612B2 (en) | 2012-07-25 | 2017-03-21 | Hitachi High-Technologies Corporation | Analysis device including solid-phase extraction material filling and discharging mechanisms |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5673347A (en) * | 1979-10-26 | 1981-06-18 | Guigan Jean | Analyser |
-
1980
- 1980-04-16 JP JP4914080A patent/JPS56147067A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5673347A (en) * | 1979-10-26 | 1981-06-18 | Guigan Jean | Analyser |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS56147067A (en) | 1981-11-14 |
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