JP3055373B2 - Liquid sample analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid sample analyzer, and more particularly to an analyzer for measuring a reaction solution by reacting a biological sample such as blood or urine with a reagent in a reaction vessel.

[0002]

2. Description of the Related Art In an apparatus for automatically analyzing a test component in a biological fluid sample, a component to be analyzed in the biological sample is generally reacted with a reagent in a reaction vessel, and the reaction solution is detected by a photometer or the like. Measurements are made in the system. The sample-treated or used reaction vessel is washed with a washing solution after draining the reaction solution, and is prepared to receive a new sample or reagent. As described in U.S. Pat.No. 4,313,735 and U.S. Pat.No. 5,037,612, a reaction vessel washing apparatus in a conventional discrete type liquid sample analyzer of this type is a reaction vessel which is intermittently transferred on a reaction line. A plurality of drain nozzles and a plurality of cleaning liquid supply nozzles are simultaneously moved forward and backward simultaneously for a plurality of reaction vessels in the row.

[0003]

The above-described conventional cleaning apparatus for an analyzer has a structure in which the same number of nozzles as the number of vessels to be processed are simultaneously and simultaneously acted on the corresponding reaction vessels. This has been a bottleneck in downsizing the analyzer. Further, since a plurality of drain nozzles and a plurality of cleaning liquid supply nozzles are provided, there is a problem that the number of parts is large and the possibility of failure is high by that much.

[0004] An object of the present invention is to provide a liquid sample analyzer that can simplify the configuration of a cleaning device.

Another object of the present invention is to provide a liquid sample analyzer which can achieve the same effect of cleaning a reaction vessel as when using a large number of drain nozzles, even though the number of drain nozzles in the cleaning device can be reduced. Is to provide.

[0006]

SUMMARY OF THE INVENTION The present invention is directed to a time interval between the start of a subsequent photometric operation, a time interval between the start of a preceding and subsequent dispensing of a sample, or a time interval between the start of a preceding and a subsequent washing operation on a reaction vessel array on a reaction line. With the time interval defined as the reference time width, the position of the drain nozzle of the cleaning device and the row of reaction vessels are relatively moved several times within this reference time width, and the same drain nozzle enters the plurality of reaction vessels. To perform a liquid discharging operation.

[0007]

In the cleaning treatment for removing the treated liquid or the reaction liquid in the reaction vessel, it is necessary to almost completely remove unnecessary residues causing the contamination from the reaction vessel thereafter. The liquid discharging operation and the cleaning liquid supply operation by the cleaning liquid supply nozzle must be performed a plurality of times, preferably three or more times, for the same reaction vessel. In the present invention, in order to execute the drainage treatment three or more times for each reaction container containing a reaction liquid that has become unnecessary,
One or two drainage nozzles are used. A plurality of types of processing are performed on a reaction vessel on a reaction line to which a row of reaction vessels is transferred in order to perform various processing on a sample at a reference time based on a predetermined time interval. These processes include sample dispensing, reagent dispensing, stirring, photometry, and washing. Various processes on the sample in the automatic analyzer are performed by repeating the reference time.

[0008] The reference time here can be the time from the start of dispensing of a sample to one container to the start of dispensing of a sample to another container on the same reaction vessel row. Alternatively, the reference time can be the time from the start of the previous photometric operation for the reaction solution formed in the reaction vessel to the start of the next photometric operation. Furthermore, the reference time can be the time from the start of the previous cleaning operation on the plurality of reaction vessels on the reaction line to the start of the cleaning operation on the plurality of reaction vessels including the new reaction vessel to be processed. The reference time can be considered as a unit of a minimum time width in which a series of processing operations in the automatic analyzer is performed by each mechanism that acts on the reaction vessel row on the reaction line,
It may be called one cycle time. The analysis operation of the automatic analyzer is advanced by repeating various processes executed at the reference time.

In the present invention, the same drainage nozzle in the cleaning device performs drainage processing on a plurality of reaction vessels within a reference time. In the embodiment of the present invention, as an example of the relative movement between the reaction vessel row and the drain nozzle, the drain nozzle is moved up and down a plurality of times at a predetermined position on the reaction line to intermittently move the reaction vessel row a plurality of times. Drainage treatment of multiple containers has been achieved. The present invention is not limited to this, and the drain nozzle is moved along the reaction container row while the reaction vessel row is stopped, and the drain nozzle is positioned in a plurality of reaction vessels to perform a drain operation. You may. The discharge nozzle may be provided with a cleaning liquid supply nozzle. Depending on the mode of sample processing, the installation position of the cleaning liquid supply nozzle and the installation position of the drain nozzle may be different on the reaction line.

Due to its necessity, the drain nozzle has a function of almost completely discharging the liquid in the reaction vessel to the outside. Therefore, the tip of the nozzle must be inserted to the deepest part in the reaction vessel. When a single drain nozzle is used in the cleaning mechanism, independent operation control according to the requirement becomes extremely easy when the nozzle drive system is operated according to the necessity / unnecessary conditions for cleaning the reaction vessel. When the reaction between the analyte in the sample and the reagent takes a long time and the analysis operation time of a specific analysis item exceeds a unit machine cycle, the corresponding reaction vessel is positioned at a predetermined washing processing position. No cleaning operation is performed. For such analysis items, information on the sample processing time or reaction time, that is, the time from the start of mixing the sample and the reagent to the end of photometry, etc.
Since the conditions have been input in advance from the input device, the control device instructs the cleaning device not to operate when the corresponding container comes to the predetermined cleaning position (both draining and cleaning liquid supply).

In a preferred embodiment of the present invention, the number of drain nozzles provided in the cleaning device is smaller than the number of reaction vessels to be drained within a reference time. The order in which the liquid drained by the drainage nozzle within the reference time is discharged is first for the reaction liquid that has already discharged the reaction liquid and contains the added cleaning liquid, and then This is for a reaction vessel containing a reaction solution that has not been washed yet. According to this, the amount of undesired substances remaining in the reaction vessel finally cleaned can be minimized.
Also, in the preferred embodiment, there is only one inherent transfer operation to transfer the reaction vessel row for sample dispensing within each reference time. On the other hand, the auxiliary transfer operation for transferring the reaction container row so that the drainage target container in the reaction container row is positioned at the drain nozzle position is performed a plurality of times.

[0012]

FIG. 1 shows the configuration of an embodiment in which the present invention is applied to an automatic biochemical analyzer. In this example, 12 reaction vessels are arranged in a loop on a rotatable reaction table 2 so that a row of reaction vessels 1 is transferred through a reaction line having a constant temperature passage controlled at a temperature of 37 degrees Celsius. ing. The reaction table 2 is intermittently rotated and transferred by a reaction table transfer device 3 whose operation is controlled by the control unit 11. The position on the reaction line where the reaction vessel 1 can be stopped is 1p
で 12p. In addition, each reaction vessel arranged as a reaction vessel row is denoted by A to L.

A description will be given of a method of performing an analysis operation when performing an original transfer operation in the analyzer. In order to execute multi-item analysis under different analysis conditions, the sample dispensing amount, reagent dispensing amount, photometric data acquisition conditions to be used for calculation, reaction in relation to each analysis item or each test component. Analysis conditions such as required time are input from the input device 30 to the control unit 11. After selectively dispensing a predetermined amount of a serum sample from the specific sample container 5 on the sampler table 6 to the reaction container A at the position 1p into the pipette 31 of the sample dispenser 4, the reaction container row on the reaction table 2 Is subjected to a plurality of auxiliary transfer operations and then to the primary transfer operation. As a result of this transfer operation, the reaction table 2 is turned counterclockwise by a distance of one circumference and one container. Therefore, the reaction vessel A stops at the position 2p, and the reaction vessel L stops at the position 1p. The time interval from the start of dispensing the sample into the reaction vessel A to the start of dispensing the sample into the next reaction vessel L is the reference time, that is, the cycle time.

A predetermined amount of reagent is selectively dispensed from the specific reagent container 7 on the sampler table 6 to the reaction container A stopped at the position 2p by the pipette 32 of the reagent dispensing device 8. During this second cycle time, a sample is dispensed into the reaction vessel at position 1p and
The stirrer of the stirrer 9 is inserted into the reaction vessel at, to execute stirring. The stirrer 9 acts on the reaction vessel A positioned at the position 3p at the next third sink time, and stirs the mixture of the sample and the reagent in the reaction vessel. Thereafter, with respect to the reaction vessel A, the luminous flux 40 of the photometric device 10 composed of a fixed photometer or the like during the original transfer operation of the reaction vessel row at each cycle time from the fourth cycle time to the eighth cycle time. Is taken into the control unit 11 in order to calculate the concentration or the enzyme activity value of the analysis item.

The four reaction vessels at positions 9p to 12p on the reaction line are subjected to the cleaning process by the cleaning device 50. The cleaning device 50 controls the cleaning process so that the cleaning process is not performed on the reaction container when the reaction container corresponding to the analysis item whose analysis operation time exceeds the unit machine cycle is between the positions 9p to 12p. The operation is controlled by the unit 11. The unit machine cycle refers to a time width required for the reaction container to return to the original position after 12 time cycles in this case. In the case of an analysis item having a long reaction time, the reaction liquid is maintained as it is at the first position to be cleaned, and the cleaning process is performed when it reaches the position to be cleaned subsequently.

The drain nozzle 12 in the cleaning device 50
Is supported by the lifting arm 15 together with the cleaning liquid dispensing nozzle 13. The arm 15 is moved up and down by an arm elevating device 14 driven and controlled by the control unit 11.
When the arm 15 is lowered, both the drain nozzle 12 and the cleaning liquid dispensing nozzle 13 enter the same reaction vessel. The tip of the drainage nozzle 12 descends to reach the lowermost end in the reaction vessel.
Is located above the liquid level in the reaction vessel. Thereby, the dispensing nozzle 13 is not brought into contact with the liquid in the reaction container. The end of the tube 51 connected to the drain nozzle 12 is opened into the drain tank 18, and the liquid sucked from the drain nozzle 12 is guided into the tank 18 by the operation of the drain pump 16. The cleaning liquid dispensing nozzle 13 is a tube 5
The cleaning liquid in the cleaning liquid tank 7 is communicated with the liquid supply pump 17 at a required timing through the liquid supply pump 17 through the nozzle 2, and is discharged from the tip opening of the nozzle 13 into the reaction vessel after draining.

Next, a description will be given mainly of how to proceed the operation when performing the auxiliary transfer operation. In the embodiment of FIG.
During one cycle time, three auxiliary transfers and one primary transfer are performed. Position this point at positions 9p-12p
2 and 3 focusing on the reaction vessel positioned in FIG.
This will be described with reference to FIG. Now, considering the N-th cycle time, this one cycle time corresponds to the case where the reaction vessel L in FIG. 1 is located at the position 12p (the vertical position of the drain nozzle 12).
At a time S1 and the subsequent movement time t for one container
1, the time S2 during which the reaction vessel K stops at the position 12p,
The subsequent movement time t2 for one container, the time S3 when the reaction container J stops at the position 12p, the subsequent movement time t3 for one container, the time S4 when the reaction container I stops at the position 12p, and so on This is the sum with the time t4 corresponding to the intrinsic transfer.

In this example, S1 to S4 are all 8 seconds, t1, t2, and t3 are each 0.5 seconds, and t4 is 2.5 seconds. Therefore, one cycle time is 36 seconds. In this example, t1 to t4
Are the same counterclockwise directions, and at t4, the reaction table is rotated by a distance corresponding to ten containers. As a result, at 1p, the reaction vessel reaches position 2p and stops at the start of the next cycle time (N + 1). However, the movement direction of t1 to t3 may be rotated counterclockwise, and the movement direction of t4 may be rotated in the opposite clockwise direction. In this case, in the movement at t4, if the distance corresponding to 14 containers is rotated, the reaction container row is in the same arrangement state as that described above.

The washing apparatus 50 operates as shown in FIG. 3 in accordance with the operation of the reaction vessel row as shown in FIG. That is, while the reaction vessels L, K, J, and I are sequentially stopped at the position 12p, the reaction vessel L is subjected to the fourth drainage processing W4 by the drain nozzle 12, and then the reaction is performed. A third drainage process W3 is performed on the container K, a second drainage process W2 is performed on the reaction container J, and finally, a first drainage process W1 is performed on the reaction container I. Is made. The top dead center in FIG. 3 indicates that the tip of the nozzle 12 is raised to a height above the upper end of the reaction vessel and does not hinder the movement of the reaction vessel row. It is in the maximum descending position where it has descended to the lowermost end of the inside. In the processes W1, W2, and W3, the cleaning liquid is supplied from the cleaning liquid dispensing nozzle 13 to the same reaction vessel after the liquid discharging operation by the liquid discharging nozzle 12 is completed. However, in the process W4, the cleaning liquid is not discharged from the cleaning liquid dispensing nozzle 13. Such an operation is controlled by the control unit 11. In this example, the liquid first discharged by the single drain nozzle 12 is the cleaning liquid added for the third time and is an extremely clean liquid. However, the last liquid discharged by the nozzle 12 in the same cycle time is the reaction liquid itself to which the cleaning liquid has not been added yet. In the next and subsequent cycle times (from N + 1), the reaction vessel row and the cleaning device perform the same operation.

Next, in the analyzer shown in FIG. 1, the distance between one vessel is moved counterclockwise in the reaction vessel row at t1, t2, and t3 within one cycle time. The analysis operation when transferring the distance between 14 containers in the direction will be described. Within the same cycle time, when the reaction vessel row is in the state of the first stop time S1,
The sample is dispensed to the reaction vessel at the position 1p by the pipette 31, the reagent is dispensed to the reaction vessel at the position 2p by the pipette 32, and the liquid in the reaction vessel at the position 3p is stirred.
Usually, the final drainage by the drainage nozzle 12 is performed on the reaction vessel at the position 12p. Container row stop time S2, S3, S
In the state of No. 4, the cleaning device 50 is placed on the reaction line.
Operates, and the drainage nozzle 12 and the washing liquid dispensing nozzle 13 are lowered to the reaction vessel at the position 12p.
The cleaning liquid is supplied by the nozzle 13 after the suction and drainage by the nozzle 2 is performed. The supply of the cleaning liquid may be performed during the raising operation of the nozzles 12 and 13. After the raising of the pair of nozzles in the stop time S4 is completed, the photometric device 10 photometrically measures the plurality of reactor vessels containing the reaction solution that has crossed the light flux 40 during the transport during the transport time t4 of the reaction vessel row. I do.

FIG. 4 shows positions (X + 2 to X-2) on the reaction line near the position X where the drain nozzle 12 moves up and down.
And what kind of stop position the reaction vessels A to D show by the auxiliary transfer within one cycle time. The reaction vessels A to D are sequentially positioned at the position X and have stop times S1 to S4. First stop time S1
At this time, the reaction vessel A receives the final drainage processing as the cleaning processing, and at the second stop time S2, the reaction vessel B receives the third drainage processing and the supply of the cleaning liquid for the container, and the third stoppage. At the time S3, the reaction vessel C receives the second drainage processing and the supply of the cleaning liquid for the vessel, and at the fourth stop time S4, the reaction vessel D discharges the reaction liquid itself, which is the first cleaning processing for the vessel. Receives liquid treatment and cleaning liquid supply.

Next, the configuration of another embodiment of the present invention will be described with reference to FIG. The apparatus in FIG. 5 is an immunoassay apparatus using a solid phase and using an enzyme as a label. Those having the same functions as those of the apparatus of FIG. 1 are denoted by the same reference numerals. As the solid phase, use is made of magnetic fine particles (having a diameter of about 1 to 5 μm), on the surface of which an antibody that specifically reacts with the immunological reaction according to the type of the test antigen (target substance) is bound. In order to perform enzyme immunoassay, first, a specific antigen in a blood sample is immunoreacted with a solid phase reagent and an enzyme-labeled antibody in a reaction vessel to form an antigen-antibody complex on a solid phase, and then an immunoreaction is performed. The bound antibody (B) and unreacted substance (F) are separated. This BF separation operation is executed by the operation of the cleaning device. The labeling enzyme on the solid phase left in the reaction vessel acts on a substrate capable of changing hue corresponding to the enzyme added thereafter. The reaction solution obtained by the enzyme reaction is measured with a photometer.

A plurality of reagent containers 7 on the reagent table 45
Includes a plurality of reagent solutions in which magnetic particles to which antibodies capable of specific reactions are respectively bound according to the type of target substance to be analyzed are dispersed and an enzyme-labeled antibody capable of binding to the target substance A solution containing a plurality of reagent solutions is prepared. The magnets 28 arranged in the vicinity of the photometric positions 7p and 8p cause the magnetic particles to be magnetically attracted to the inner wall of the container away from the light beam passage so that the solid phase of the magnetic particles in the reaction container does not disturb the photometry during photometry. . Magnet 2 arranged near the position 14p, 15p, 16p of the container to be cleaned
0 is for magnetically adsorbing the solid phase to the inner wall of the vessel so that the solid phase is not discharged from the reaction vessel during the washing process. The enzyme substrate dispensing nozzle 21 and the buffer solution dispensing nozzle 22 that move up and down at the position 19p on the reaction line by the vertical movement of the arm 23 can simultaneously enter the same reaction vessel. The enzyme substrate liquid from the substrate tank 26 is discharged from the substrate dispensing nozzle 21 into the reaction container by the liquid sending pump 25. The buffer solution from the buffer solution tank 27 is discharged from the buffer solution dispensing nozzle 22 into the reaction container by the solution sending pump 24.

In the analyzer shown in FIG. 5, 31 reaction vessels are arranged on a reaction table 2 on a reaction line, and a row of reaction vessels rotates by a distance corresponding to 16 vessels within one reference time, that is, a cycle time. Is done. In the cleaning process within each cycle time, the operation of each nozzle 12, 13 is the same as that shown in FIGS. Position 1 on the reaction line
A desired blood sample is dispensed by the sample dispenser 4 into the reaction vessel p, and the solid phase dispersion reagent and the enzyme labeling reagent are dispensed by the reagent dispenser 8 into the reaction vessel at position 2p. With the repetition of the cycle time, the positions on the reaction line of the reaction vessel that are stopped at the first stop time S1 are those at the initial position 1p at 17p, 2p, and 18p.
... in that order.

At the start of the cleaning process, that is, at the start of the X-th cycle time, the reaction vessel B that has reached the position 13p on the reaction line is stopped at the position 16p in the fourth stop time S4 in that cycle time, and the cleaning liquid Injection nozzle 1
The washing liquid is dispensed from 3. At this point, the drain nozzle 1
No. 2 does not perform the discharging operation. The reaction vessel B reaches the position 29p at the time of the first stop time S1 in the next cycle time, and is subjected to the stirring process by the stirring device 9. And the next X
In the third stop time S3 in the second cycle time, the reaction vessel B is stopped at the position 16p, and in a state where the solid phase is adsorbed by the magnet 20 on the reaction vessel wall, first, the drainage processing by the drainage nozzle 12 is performed. receive. As a result, the liquid containing the unbound substance with respect to the solid phase is discharged to the drain tank 18. The cleaning liquid is dispensed from the cleaning liquid dispensing nozzle 13 to the reaction vessel B where the solid phase remains in the same stopped state. X + 2
In the fourth stop time S4 in the fourth cycle time, the reaction vessel B reaches the stop 17p, so that the influence of the magnetic field is reduced, and the solid phase adsorbed on the inner wall of the vessel starts to disperse in the cleaning solution.

Next, in the X + 3rd cycle time, the reaction vessel B reaches the position 30p in the stop time S1, and the stop time S
2, the position 31p is reached, the stop time S3 reaches the position 1p, and the stop time S4 reaches the position 2p. While the reaction vessel B is at the positions 30p and 31p, the liquid phase in the reaction vessel B is stirred by the stirrer 9 so that unnecessary substances adhering to the surface of the solid particles are diffused into the cleaning liquid. The phase particles are dispersed in the liquid phase. Further, at the (X + 4) th cycle time, the reaction vessel B reaches the position 15p at the first stop time S1, and reaches the position 16p at the second stop time S2. At these positions, the solid phase is adsorbed on the inner wall of the reaction vessel by the magnet 20. At the position 16p, first, the cleaning liquid is discharged from the reaction container B by the drain nozzle 12 so that the solid phase remains in the container, and then the cleaning liquid dispensing nozzle 1
The washing liquid is added to the reaction vessel B from 3. In the X + 5th cycle time, the reaction vessel B is positioned at the first stop time S1.
After reaching 31p, the mixture is stirred by the stirrer 9 so that the solid phase is dispersed in the washing liquid. In the next (X + 6) th cycle time, when the reaction vessel B reaches the position 16p during the first stop time S1, the liquid is almost completely discharged by the drain nozzle 12 while the solid phase remains in the reaction vessel B. In the fourth stop time S4 in the same X + 6th cycle time, the reaction vessel B is stopped at the position 19p, the enzyme substrate solution is added from the substrate dispensing nozzle 21, and the buffer solution is added from the buffer solution dispensing nozzle 22. Thereby, the substrate is acted on by the enzyme bound on the solid phase remaining in the reaction vessel B, and the enzymatic reaction starts.

In the X + 7th cycle time,
During the first stop time S1, the reaction vessel B is stopped at the position 1p, but nothing is added to the reaction vessel B here. That is,
The control unit 11 controls the sample dispensing device 4 so that it does not operate. First stop time S at each cycle time
In the case of focusing on No. 1, the reaction vessel B moves the positions 17p, 2p, 18p, 3p,
Change in the order of 19p, 4p, 20p, 5p. The magnet 28 is adsorbed to a location on the inner wall of the reactor that does not interfere with the measurement of solid particles in the reactor at positions 7p and 8p. First stop time S1
The reaction vessel that has reached the position 5p at the time is associated with the luminous flux of the photometric device 10 in the fourth stop time S4 within this cycle time, and the first photometry is performed. The photometric treatment measures the fluorescence or absorbance of the reaction solution after the enzymatic reaction. Further, when the third stop time S3 of the cycle time after two times, the second stop time S2 of the cycle time after four times, and the first stop time S1 of the cycle time after six times, the reaction vessel B is at the position 8p. Since they are associated with each other, photometry is performed four times in total. The photometric data is subjected to arithmetic processing in the control unit 11 and displayed on the display device as the target substance concentration in the sample.

As described above, in any of the embodiments shown in FIGS. 1 and 5, the drain nozzle of the cleaning device is merely moved up and down, and no horizontal operation is required. Therefore, the nozzle drive mechanism is extremely simple. I'm done. Further, a single drain nozzle can be used for discharging unnecessary liquids from three or four reaction vessels within the same reference time (cycle time), and the cleaning apparatus can be made extremely small. In addition, since only one reaction vessel is required to correspond to the nozzle of the cleaning apparatus, individual control such as changing the nozzle operation according to the condition of the analysis item is facilitated.

[0029]

According to the present invention, since the structure of the cleaning mechanism can be simplified, it is possible to contribute to downsizing of the analyzer, and the possibility of failure is reduced by reducing the number of components. Further, although the number of drainage nozzles in the cleaning device can be reduced, the same cleaning effect as when a large number of drainage nozzles are used can be obtained.

[Brief description of the drawings]

FIG. 1 is a diagram showing a schematic configuration of an embodiment of the present invention.

FIG. 2 is a view for explaining a reaction vessel cleaning operation within one cycle time of the analyzer of FIG. 1;

FIG. 3 is an explanatory diagram of an operation of a cleaning nozzle system in the analyzer of FIG. 1;

FIG. 4 is an explanatory diagram of a processing status of a plurality of reaction vessels in one cycle time.

FIG. 5 is a diagram showing a schematic configuration of another embodiment of the present invention.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Reaction container, 2 ... Reaction table, 10 ... Photometric device, 1
2: Nozzle for drainage, 13: Dispensing nozzle for cleaning liquid, 20, 2
8: magnet, 21: substrate dispensing nozzle, 22: buffer solution dispensing nozzle.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-6-82461 (JP, A) JP-A 54-81388 (JP, U) (58) Fields investigated (Int. Cl. ⁷ , DB name) G01N 35/00-35/10

Claims (6)

(57) [Claims] 1. An intermittent transfer of a row of reaction vessels on a reaction line,
In a liquid sample analyzer that starts a photometric operation at a predetermined time interval with respect to a reaction vessel in which a reaction solution has been formed, and cleans the reaction vessel after the photometry is completed, a single discharge of the washing apparatus is performed at a predetermined position on the reaction line. The liquid nozzle is configured to move up and down, and during the predetermined time interval, the reaction container row is moved a plurality of times to position the reaction container to be processed at the predetermined position, and the single drain nozzle is Wherein the liquid in the plurality of reaction vessels is discharged. 2. The analyzer according to claim 1, wherein the drain nozzle discharges a reaction liquid from a reaction container containing the reaction liquid and performs a reaction from another reaction container within the predetermined time interval. A liquid sample analyzer that performs a cleaning liquid discharging operation. 3. From the start of dispensing a sample to a specific reaction container positioned at a sample dispensing position on a reaction vessel row, and thereafter from the start of dispensing a sample to another reaction container positioned at the sample dispensing position. In the liquid sample analyzer that dispenses samples, dispenses reagents for reaction, and cleans the reaction container within the reference time, the reaction container to be drained within the reference time is set as the reference time. The number of drain nozzles smaller than the number is provided, and the reaction vessel row is moved a plurality of times within the reference time, and the liquids in a plurality of different reaction vessels are discharged by the same drain nozzle. Characteristic liquid sample analyzer. 4. The analyzer according to claim 3 , wherein the primary transfer operation of the reaction vessel row for positioning the reaction vessel for dispensing a sample is performed only once within the reference time, and the liquid is transferred to the drain nozzle. 2. The liquid sample analyzer according to claim 1, wherein the auxiliary transfer operation of the positioning reaction vessel row is performed more times than the number of the drainage nozzles within the reference time. 5. A liquid sample analyzer for performing a washing operation on a reaction vessel on a reaction line, wherein a reference time interval from the start of washing of a previous reaction vessel to the start of washing of a new next reaction vessel is provided. The position of the drainage nozzle of the cleaning device and the row of the reaction vessels are relatively moved a plurality of times, and the same drainage nozzle enters the plurality of reaction vessels to perform a liquid discharge operation. Liquid sample analyzer. 6. The analyzer according to claim 5, wherein the liquid discharging operation by the same liquid discharging nozzle is performed after the reaction vessel to which the reaction liquid has been discharged and the cleaning liquid has been added. A liquid sample analyzer characterized by being sequenced to be executed on a reaction vessel containing a reaction solution.