CN107942085A - Automatic analysing apparatus and its method of sample analysis - Google Patents

Automatic analysing apparatus and its method of sample analysis Download PDF

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Publication number
CN107942085A
CN107942085A CN201710979179.3A CN201710979179A CN107942085A CN 107942085 A CN107942085 A CN 107942085A CN 201710979179 A CN201710979179 A CN 201710979179A CN 107942085 A CN107942085 A CN 107942085A
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China
Prior art keywords
reaction vessel
unit
reagent
reaction
processing unit
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CN201710979179.3A
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Chinese (zh)
Inventor
张震
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Shenzhen Kai Kai Biological Technology Co Ltd
Shenzhen Increcare Biotech Co Ltd
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Shenzhen Kai Kai Biological Technology Co Ltd
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Application filed by Shenzhen Kai Kai Biological Technology Co Ltd filed Critical Shenzhen Kai Kai Biological Technology Co Ltd
Priority to CN202310888939.5A priority Critical patent/CN116840499A/en
Priority to CN201710979179.3A priority patent/CN107942085A/en
Priority to CN202010348995.6A priority patent/CN111551758A/en
Publication of CN107942085A publication Critical patent/CN107942085A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • G01N2035/0401Sample carriers, cuvettes or reaction vessels
    • G01N2035/0437Cleaning cuvettes or reaction vessels

Abstract

The present invention provides a kind of automatic analysing apparatus and method of sample analysis.The automatic analysing apparatus includes:Unit (20) is filled, fills sample or/and reagent to reaction vessel;Hatch unit (80), the reactant in incubation reaction container;Processing unit (10), cleaning separate the reactant in reaction vessel and measure the reaction signal in reaction vessel;Buanch unit (50), between the different positions transfer reaction container.The present invention realizes the incubation of reactant in reaction vessel centered on hatch unit, independently of the measurement of signal in cleaning separation and reaction vessel that reactant in reaction vessel is realized centered on the processing unit of hatch unit.The present invention not only eliminates individually cleaning separator disk and Measuring plate, has simplified system structure and control flow, can also significantly reduce processing unit and hatch unit size, and realize flexible incubation time.

Description

Automatic analysing apparatus and its method of sample analysis
Technical field
The present invention relates to in-vitro diagnosis apparatus field, and in particular to a kind of automatic analysing apparatus and its method of sample analysis.
Background technology
In recent years, the development and progress of clinical examination and automatic technology, not only improves clinical laboratroy automation water It is flat, the efficiency of medical test is improved, also improves the q&r of inspection result.However, with detection specimen amount Increase, clinical labororatory needs constantly to increase Large-Scale Automatic Measurement System to meet its detection demand, so as to cause laboratory Increasingly crowded and testing cost is constantly soaring.Thus, how in the case where facing growing cost pressure, lifting checkability, Ensure result and make full use of existing lab resources, be the clinical examination pressing issues to be solved.
In order to express easily, exempted from herein with full-automatic in in-vitro diagnosis (In-Vitro Diagnostics, abbreviation IVD) Epidemic disease analyzer, especially, using luminescence immunoassay instrument as row, illustrates the technical program and method, those skilled in that art should The understanding, the present invention program and method can also be used for other clinical examination automation equipments, such as fluorescence immunoassay device, electrochemistry It is immunized.Automatic immunity using enzyme by based on the immunological response that antigen-antibody be combined with each other, being marked, group of the lanthanides Rubidium marking or chemiluminescent agent labelled antigen antibody, are reacted by a series of Cascaded amplifications, by optical signal or electric signal with dividing Analysis thing concentration etc. is associated, and analyzes the antigen or antibody to be measured in human sample, be mainly used in hospital clinical laboratory, the 3rd The mechanisms such as square independent laboratory, blood examination center, carry out each analyte in Human Fluids quantitative, sxemiquantitative or qualitative detection, Carry out the diagnosis of infectious disease, tumour, endocrine function, angiocardiopathy and prenatal and postnatal care and autoimmunity class disease etc..Entirely Automated immunoassay analyzer is usually made of sampling unit, reaction member, supply and waste waste unit, system control unit etc.. Electrochemiluminescent immunoassay due to quantitative detection, high sensitivity, the advantage such as specificity is good, the range of linearity is wide, the degree of automation is high just into For the mainstream technology that automation at present is immune.Full-automatic illumination immunoassay is different with luminescence system according to labeling method, and wraps Include enzyme-catalyzed chemical luminescence, direct chemiluminescence, electrochemical luminescence etc..
Refer to the attached drawing 1-3, luminescence immunoassay by test philosophy and pattern generally can be divided into one-step method, delay one-step method, Two-step method etc., main testing procedure generally comprises filling sample and reagent, reactant are mixed, be incubated, cleaning separates (Bound- Free, abbreviation B/F), plus signal reagent, measurement etc..It is pointed out that in order to express easily, the present invention distinguished reagent and Signal reagent, incubation and signal are incubated.Reagent and analysis project are correspondence " one by one ", i.e., the corresponding tool of different analysis projects Body reagent is general different in formula, amount of reagent, number of components etc..According to the difference of concrete analysis project, reagent usually wraps Multiple components are included, such as 2-5 common component, including the reagent component such as magnetic particle reagent, enzyme marking reagent, dilution.According to anti- Answer pattern different, multiple reagent components of an analysis project, which can be filled disposably, can also divide multiple steps to fill, substep During rapid filling the first reagent, the second reagent, the 3rd reagent etc. are defined as according to filling order.Signal reagent is used for measuring signal Produce, be usually one kind of common reagent, with the correspondence that analysis project is " one-to-many ", i.e., different analysis projects shares Signal reagent.The incubation of the present invention is refered in particular to reaction vessel and is started before cleaning separation, constant temperature of the reactant in it in hatch unit The antigen-antibody binding reaction or the process of biotin-labeled pentylamine association reaction occurred under environment, specifically, one-step method are incubated one Secondary, to enter the once incubation before cleaning separation, delay one-step method is incubated twice, including the first time before the second reagent of filling incubates Educate and enter second of incubation before cleaning separation after filling the second reagent, two-step method is incubated twice, including cleaning point for the first time It is incubated from preceding first time and cleans second of incubation before separation for second.And the reaction that signal is incubated after referring to cleaning separation is held Device reacts a period of time after signal reagent is added under isoperibol, makes the process of signal enhancing.According to reaction system and hair The difference of light principle, not all test is required for signal to be incubated, it is necessary to which the test that signal is incubated is generally enzymatic class chemistry Luminescence immunoassay.Details are as follows for the corresponding testing procedure of different test patterns:
1) one-step method:Refer to the attached drawing 1, filling sample (S) and reagent (R), (some test methods can not also need for mixing Mix, similarly hereinafter, repeat no more), (generally 5-60 minutes) are incubated, cleaning separation is carried out after the completion of incubation, fills signal reagent, Signal is incubated (generally 1-6 minutes), finally measures.It is pointed out that due to the difference of the specific component of signal reagent, some Luminescence system undesired signal is incubated, and can directly be measured during signal reagent is filled or after having filled signal reagent.Letter Number reagent can be one or more, and refer to the attached drawing 2, signal reagent includes the first signal reagent, secondary signal reagent.
2) be delayed one-step method:It is that reagent fills at twice with one-step method difference, adds the first reagent to be carried out after mixing It is incubated for the first time, the second reagent is added after the completion of being incubated for the first time and is mixed.It is more than once compared with one-step method to be incubated, fill reagent Acted with mixing, remaining flow is as one-step method.
3) two-step method:It is more than once cleaning separating step with delay one-step method difference, other steps are identical.
In order to realize above-mentioned process automation test, existing specific implementation technical solution is as follows:
The first prior art will be incubated, cleaning separation and measurement separate separated layout, respectively by three rotational circles Disk completes corresponding function, and reaction vessel is completed to shift by mechanical gripping arm between different units takes place.The technical solution component and unit More, reaction vessel needs the problems such as being shifted between each unit, causing volume big, of high cost, control flow is complicated.
Second of prior art will be incubated and measurement is arranged together composition and is incubated measuring unit, and cleaning separation is by another One separate unit is completed, although compared with the first existing scheme, which reduces a measurement disk, certain Be conducive to control package size and cost in degree, but equally exist the problem of identical with the first technical solution.The technical side For case in order to realize flexible incubation time, incubation measuring unit control is complicated, is incubated and measurement also can be restricted mutually in control, The shortcomings of can not realizing high-speed automated test is not only existed, can not realize that flexible signal is incubated yet.
The third prior art will be incubated, cleaning separation and measurement are real on an individual pen disk or discrimination shape track Existing, for the program in order to support longer incubation time, disk is in addition to cleaning separation and measurement position, it is also necessary to sets very much Position is incubated, so in order to realize high speed test, disk or discrimination shape track size need to be designed to very big, manufacturing difficulty Greatly, it is of high cost, in addition, in order to realize delay one-step method and two-step method test, it is also necessary at least two sample charging mechanisms and at least two A cleaning separator, so as to add material, processing, production cost and package size.On the other hand, which also limits Incubation time has been made, incubation time has been result in and fixes, goes out the problems such as result overlong time.In addition, the technical solution is difficult not only The dark room conditions needed for measurement are realized, it is necessary to increase extra tripper, can not also realize that flexible signal is incubated.
The content of the invention
The shortcomings that to solve the problems, such as prior art generally existing and, the present invention provides that a kind of manufacturing cost is low, structure Simple and compact, testing process or the flexible and efficient automatic analysing apparatus and its method of sample analysis of method.
According to an aspect of the present invention, there is provided a kind of automatic analysing apparatus, including:Unit is filled, fills sample or/and examination Agent is to reaction vessel;Hatch unit, the reactant in incubation reaction container;Processing unit, cleaning separate anti-in reaction vessel Answer thing and measure the reaction signal in reaction vessel;Buanch unit, between the different positions transfer reaction container;It is described to be incubated list Member includes rotating device, incubation reaction container position is set on the rotating device, for the reactant in incubation reaction container;Institute Stating processing unit includes processing disk, sets cleaning separation reaction vessel position and measurement reaction vessel position on the processing disk, respectively For cleaning the reaction signal in reactant and measurement reaction vessel in separation reaction vessel.
In accordance with a further aspect of the present invention, there is provided a kind of method of sample analysis, including:Step is filled, into reaction vessel Fill sample and reagent;Incubation step, is incubated the reaction vessel on the reaction vessel position of hatch unit;Incubation step, Reactant in reaction vessel on the reaction vessel position of hatch unit is incubated;Separating step is cleaned, to processing unit Cleaning separation reaction vessel position on reaction vessel in reactant carry out cleaning separation;Measuring process, to processing unit The reaction signal in reaction vessel on measurement reaction vessel position measures;Transfer step, will be described anti-by buanch unit Container is answered at cleaning separation reaction vessel position, the place of the hatch unit and the processing unit and the processing unit Shifted between the measurement reaction vessel position of reason unit.
The hatch unit of the present invention realizes the incubation of reactant in reaction vessel, real independently of the processing unit of hatch unit The cleaning separation of reactant and the signal in reaction vessel is measured in existing reaction vessel, reaction vessel in hatch unit and Buanch unit realization is displaced through between processing unit.The cleaning separation and measurement of the present invention on a process disk inside and outside respectively Realized on circle, not only eliminate individually cleaning separator disk and Measuring plate, also improve the reliability of reaction vessel transfer, so that Simplify system structure and control flow, significantly reduced hatch unit and processing unit size, and pass through independent incubation list Member realizes flexible incubation time.The present invention improves the work efficiency of analytical equipment, solves current self-reacting device very well The technical barriers such as volume is big, detection speed is slow, of high cost, poor performance, have not only saved lab space, improve test effect Rate, and expenses is advantageously reduced, finally save substantial amounts of natural resources and social resources.
Brief description of the drawings
Fig. 1 is one-step method reaction pattern schematic diagram;
Fig. 2 is one-step method reaction pattern (another signal measurement mode) schematic diagram;
Fig. 3 is delay one-step method and two-step method reaction pattern schematic diagram;
Fig. 4 is the first embodiment schematic diagram of automatic analysing apparatus of the present invention;
Fig. 5 is one-step method test flow chart;
Fig. 6 is delay one-step method test flow chart;
Fig. 7 is two-step method test flow chart;
Fig. 8 is second of embodiment schematic diagram of automatic analysing apparatus of the present invention.
Embodiment
The present invention is described in further detail below by embodiment combination attached drawing.
A kind of automatic analysing apparatus of the present invention, including:Unit is filled, fills sample or/and reagent to reaction vessel;Incubate Educate unit, the reactant in incubation reaction container;Processing unit, cleaning, which separates the reactant in reaction vessel and measures reaction, to be held Reaction signal in device;Buanch unit, between the different positions transfer reaction container;The hatch unit includes rotating device, Incubation reaction container position is set on the rotating device, for the reactant in incubation reaction container;The processing unit includes Disk is handled, cleaning separation reaction vessel position and measurement reaction vessel position are set on the processing disk, it is anti-to be respectively used to cleaning separation Answer the reaction signal in the reactant and measurement reaction vessel in container.
Reaction vessel provides reacting environment for the reaction of sample and reagent, can be it is variously-shaped and construction reaction tube, Reaction cup, the reaction cup bar of multiple chambers, reaction chip etc., it is generally disposable.The material of reaction vessel is usually plastics, Such as polystyrene.Reaction vessel be able to can not be also coated with the advance envelope antigen of inner wall or antibody, can also be deposited in advance in it Put the magnetic bead or baton round being coated with.The storage and supply of reaction vessel are completed by reaction vessel feed unit.Reaction vessel supplies Using two kinds of major way storages and reaction vessel mainly is provided to unit, one kind is bin type, and reaction vessel can be dissipated into bag Disorderly pour into the feed bin of reaction vessel feed unit, then reaction vessel feed unit is automatically by reaction vessel gradually single row Sequence, supply response container to buanch unit;Another way is walkthrough column, and reaction vessel is arranged in reaction vessel support in advance In disk, box or reaction vessel holder, conduit, reaction vessel feed unit every time can be by whole disk, whole box reaction vessel or a row, a row Reaction vessel is delivered to target location.
Transfer of the reaction vessel in apparatus of the present invention between diverse location can be completed by buanch unit.Buanch unit can be with It is any suitable can shift or the mechanism of mobile response container, currently preferred buanch unit mainly include driving machine The structures such as structure, horizontal movement machinery arm, pick-and-place mechanism.Pick-and-place mechanism is usually mechanical finger, can be horizontal with pick-and-place reaction vessel Movable machinery arm under driving mechanism driving can along X to, Y-direction, X be to Y-direction, radially, circumferentially, radially and circumferentially etc. direction is moved Dynamic pick-and-place mechanism, diverse location is moved to by the reaction vessel that pick-and-place mechanism captures.In addition to horizontal movement, buanch unit may be used also Move up and down, reaction vessel is put into different positions or is taken out from different positions.It is laid out according to test speed and complete machine Difference, can set one or more buanch units.
Fill the filling that unit completes sample, reagent.Unit is filled generally by draw point or disposable suction nozzle (Tip), filling Motion driving mechanism, syringe or topping-up pump, valve, fluid circuit and rinse bath (can also not have rinse bath when using Tip) Formed Deng component.In order to complete to draw sample, reagent and its filling action, filling unit may be used also in addition to it can move up and down With horizontal movement, horizontal movement usually has rotation, X to several forms of motion such as, Y-direction and combinations thereof.It can be one to fill unit It is a, this but also reagent adding be not only loaded, can so make complete machine structure more compact lower with cost.In order to improve test speed, filling is single Member can also further comprise one or several sample filling units, one or several reagents filling unit, and sample filling unit is only Fill sample or filling sample and portion of reagent, reagent filling unit filling reagent.
In order to facilitate the filling of filling unit, the present invention may also include filling station.Filling station is located at buanch unit and filling It can be moved in the range of movement of unit or by horizontal movement in the range of movement of buanch unit and filling unit.Filling station connects Receive and carrying buanch unit shifts the reaction vessel to come, receiving filling unit fills sample and reagent into reaction vessel.Add Note sets reaction vessel position on station, for placing the reaction vessel for needing to fill sample and reagent.In order to mix sample and reagent Close evenly, reaction it is more abundant, while in order to simplify complete machine structure and reduce volume, mixing mechanism can be integrated in filling station, it is right The ultrasonic mixing of reaction vessel progress, deviation rotation or concussion mixing after filling every time, can also be by mixing mechanism, such as ultrasound Wave producer is integrated in filling unit, is produced while sample and reagent is filled or after the completion of filling action by filling unit Ultrasonic wave, which is realized, to be mixed.Those skilled in that art are appreciated that filling station can not also integrate mixing mechanism, and mixing can also be by adding The suction action or impact force for noting unit are completed.In order to make complete machine more compact, filling station can be also monolithically integrated in hatch unit, So filling station can not be located under the track of buanch unit.
Reactant in hatch unit incubation reaction container.Hatch unit mainly includes attemperator and rotating device.Incubate The attemperator periphery for educating unit usually has the heat-barrier materials such as heat-preservation cotton, usually wraps up or surrounds the bottom of rotating device, week Side and top, side or bottom inner side can be equipped with heating unit and sensor, and top is generally the structures such as cover board, is hatch unit Constant-temperature incubation environment is provided.Certainly, in order to which heat transfer efficiency higher, heating unit can also be installed on the spinning device.It is incubated single Several holes, groove, bracket, base or other structures for being adapted to carry reaction vessel are set on the rotating device of member, are defined as anti- Answer container position.Reaction vessel position is the main place that the reactant reaction in reaction vessel is incubated, and makes the sample in reaction vessel React to each other between analyte and corresponding reagent and reagent and reagent.Usually, reaction vessel position is more, supported Incubation time is longer, and test speed is also faster.Hatch unit size, hatch unit are controlled at the same time in order to increase reaction vessel position Rotating device at least two circle reaction vessel positions using rotating device pivot as the center of circle can be set.Rotating device is preferably One, including driving, transmission mechanism and relevant control circuit etc., control and drive rotating device to need to rotate according to test, Transfer some positions of reaction vessel position advance.The movement of at least one reaction vessel position on rotating device in buanch unit In the range of, reaction vessel can be moved in and out the rotating device of hatch unit by such buanch unit.
The reaction signal in reactant and measurement reaction vessel in processing unit cleaning separation reaction vessel.Processing unit Mainly include attemperator, processing disk, cleaning separator and measuring device.The attemperator of processing unit and hatch unit class Seemingly, periphery usually has a heat-barrier material such as heat-preservation cotton, usually parcel or surrounds bottom, periphery and the top of processing disk, side or Bottom inside can be equipped with heating unit and sensor, and top is generally the structures such as cover board, constant-temperature incubation ring is provided for processing unit Border.Certainly, in order to which heat transfer efficiency higher, heating unit can also be installed on the spinning device.In addition to isoperibol is provided, protect The magnetic field generation device of the also sustainable and fixed cleaning separator of warm device, magnetic field environment is provided for cleaning separation.In addition, by In needing the signal that measures to be usually faint optical signal, when test be easily subject to ambient light interference and influence and measurement is not allowed Really, attemperator can also provide closed dark room conditions required during measurement for the measuring device of processing unit.With hatch unit It is similar, handle and several holes, groove, bracket, base or other structures for being adapted to carry reaction vessel are set on disk, be defined as reacting Container position, wherein being defined as cleaning separation reaction vessel position for cleaning separated reaction vessel position, the reaction for measurement is held Device position is defined as measurement reaction vessel position.Reaction vessel position on processing disk can be set to using the processing disc spins center as circle At least two circles of the heart, cleaning separation reaction vessel bit distribution is at least one circle, and measurement reaction vessel bit distribution is at least in addition On one circle, the size and volume that interfere with each other and reduce processing unit of cleaning separation and measurement are so reduced.For needing The test that signal is incubated, measurement reaction vessel position can also realize that signal is incubated in addition to for measuring.By the rotation for handling disk Turn, the reaction vessel on measurement reaction vessel position can be rotated to measuring device and measured, be incubated so as to fulfill signal.With enzyme Exemplified by promoting chemiluminescent signal incubation, if its signal, which is incubated, needs 5 minutes, measurement reaction vessel position can transfer reaction vessel It is incubated to the signal that 5 minutes are realized during measuring device.It is preferably one to handle disk, including driving, transmission mechanism and phase Control circuit of pass etc., control and drive processing disk are every set time (such as a circulation or cycle) the fixed angle of rotation Degree, transfers reaction vessel position thereon and advances some positions (such as reaction vessel position of advancing).On rotation processing disk extremely In the range of movement of buanch unit, reaction vessel can be moved in and out processing by such buanch unit for few two reaction vessel positions Disk.
For processing unit in addition to above-mentioned function, cleaning separator thereon can also be on cleaning separation reaction vessel position Reaction vessel carry out cleaning separation, with remove in reactant be not associated with component.The cleaning separation dress of processing unit of the present invention Put including magnetic field generation device and flushing machine.Magnetic field generation device provides magnetic field environment, makes the paramagnetic particle in reaction vessel It is adsorbed onto reaction vessel inner wall.Due to the factors such as the response time in magnetic field, displacement distance and resistance, paramagnetic particle absorption The regular hour is needed to reaction vessel inner wall, is usually to differ for several seconds to tens seconds, is so drawing waste liquid every time (including not Binding constituents) before, reaction vessel is needed by magnetic field for a period of time.In a preferred embodiment of the present invention, magnetic field produces dress The attemperator of processing unit can be mounted directly or be fixed on by putting, and so can not only be saved extra fixed mechanism, be reduced into This, can also make magnet generation device closer to reaction vessel position, so as to reduce the adsorption time of paramagnetic particle, improve cleaning separation Efficiency.Flushing machine includes imbibition and priming device, aspirates uncombined component in reaction vessel and into the reaction after suction Inject cleaning buffer solution.Liquid absorption device includes the imbibition portion of the suitable pumping liquids such as aspirating needle, pipette or imbibition nozzle, imbibition portion The top of processing unit is arranged in, can be anti-by the reaction vessel on the drive disengaging reaction vessel position of driving mechanism, suction Answer the uncombined component in container.Priming device includes the fluid injection portion of suitable row's fluid injection bodies such as injection needle, pipe, mouth, and fluid injection portion is same Sample is arranged in the top of the reaction vessel position of processing unit, injects cleaning buffer solution into the reaction vessel after suction.Punching every time Wash bags include an imbibition and once inject cleaning buffer solution and process, generally flush three times or four times, that is, carry out three times or four times Rinse, certain washing time also can be flexible and changeable.In order to make cleaning more thorough, residual is less, can also set and mix in fluid injection position Impact force when device mixes reaction vessel or utilizes fluid injection, makes paramagnetism at the same time or after note cleaning buffer solution in note cleaning buffer solution Particle resuspension and it is dispersed in cleaning buffer solution.Processing unit processes disk transfers reaction vessel to cleaning separator When, cleaning separator starts to carry out cleaning separation to reaction vessel.In addition, in order to streamline organizations, cleaning separator may be used also Further coupled signal reagent filling mechanism, after reaction vessel completes cleaning separation, to its interior all or part of signal of filling Reagent, for example fill first, second whole signal reagent etc. or only fill first signal reagent etc., remaining signal reagent can Filled in measurement.The function of cleaning separator can be so made full use of, organization volume is reduced and saves cost.Clearly Processing disk periphery or top that separator is arranged in processing unit are washed, directly the reaction vessel on processing disk can be carried out clear Separation is washed, so can be to avoid setting independent cleaning to separate rotating device, such as independent cleaning separator disk or cleaning separate rail Road etc., has not only simplified component and complete machine mechanism, makes complete machine mechanism more compact lower with cost, it is thus also avoided that reaction vessel is only Transfer between vertical cleaning separator and hatch unit, makes complete machine control flow simpler efficient, so as to improve processing effect Rate and reliability.
The measuring device of processing unit measures the signal in reaction vessel.Signal is addition signal in reaction vessel Electric signal, fluorescence signal or faint chemiluminescence signal for being produced after reagent etc..Measuring device includes dim light detector photoelectricity The optical signal of measurement, can be converted to electric signal, be sent in control by multiplier tube (PMT) or other sensitive optoelectronic induction devices The heart.In addition, in order to improve measurement efficiency and ensure measurement uniformity, measuring device can also further comprise optical signal collection and school The Optical devices such as standard.In a preferred embodiment of the present invention, measuring device can be connected or attached to place by generic way On the attemperator for managing unit, for example it is mounted directly and is fixed on attemperator or attemperator is connected to by optical fiber On, directly the signal in the reaction vessel on processing disk measurement reaction vessel position can so be measured, avoid setting only Vertical measurement position or Measuring plate, can make complete machine mechanism is more compact, cost is lower, control flow more it is simple efficiently, treatment effeciency With reliability higher.In addition, the filling of signal reagent for convenience, processing unit of the invention may also include signal reagent filling Mechanism, signal reagent is filled into the reaction vessel on processing disk reaction vessel position.The processing unit of the present invention is independently of incubation Unit, not only cleaning separation and measurement easy to implement, can also realize flexible incubation time, solve prior art package size The shortcomings of excessive, complicated, incubation time is fixed.
In addition, in order to convey sample and storing reagent, automatic analysis apparatus of the present invention can also set sample supply unit, The units such as reagent storage unit.
Sample supply unit is used to place sample tube to be checked and target sample pipe is delivered to suction sample bit.Sample conveying is single Member has three kinds of track sample introduction, sample disk sample introduction and fixed area sample introduction major ways, and sample tube is generally positioned on sample rack, often A sample rack generally places 5 or 10 sample tubes, and sample rack is positioned on transmission rail, on sample disk or analytical equipment is consolidated Determine region.
Reagent storage unit, which refrigerates reagent and transfers to destination agent, inhales reagent position.Reagent storage unit generally use is tried Agent disk and agent bin two ways, in order to ensure the stability of reagent, reagent disc generally has refrigerating function, such as 4~10 DEG C.Examination Several reagent container positions are generally set on agent disk, for placing reagent container.Each reagent container sets several independent Cavity, for storing different reagent components, such as magnetic particle reagent, enzyme marking reagent, dilution reagent component.
The first embodiment of automatic analysing apparatus of the present invention, with reference to figure 4.It is defeated that automatic analysing apparatus 100 includes sample Send unit 30, reagent storage unit 40, filling unit 20, reaction vessel feed unit 70, buanch unit 50, processing unit 10, Hatch unit 80 and filling station 90 etc..Function and the effect of each several part is described separately as below.
Sample supply unit 30 is used to place sample tube 31 to be checked and target sample pipe is delivered to suction sample bit.This implementation In example, sample supply unit 30 is sample disk, is placed on sample disk on arc sample rack (not marked in figure), each arc sample Frame places 10 sample tubes 31.Sample disk can be driven by driving mechanism under the control of control centre target sample transferring to suction Sample bit, inhales sample bit positioned at the horizontal movement track of filling unit 20 and the point of intersection of sample tube center circle.
Reagent storage unit 40, which refrigerates reagent container 41 and transfers to destination agent, inhales reagent position.In the present embodiment, examination Agent storage unit 40 is reagent disc, sets 16 reagent positions, and can accommodating 16 reagent containers 41, (or kit, reagent bottle are table State conveniently, hereinafter referred to as reagent bottle).In the present embodiment, each reagent bottle 41 sets 4 cavitys 41a, 41b, 41c, 41d, can use In reagent components such as storage magnetic particle reagent, enzyme marking reagent, dilutions.Reagent disc can be under the control of control centre by driving machine Structure, which drives to transfer in destination agent bottle, inhales reagent position, inhales reagent position positioned at filling unit horizontal movement locus and reagent chamber center Round point of intersection, it is corresponding with corresponding 4 reagent components in the present embodiment, there are 4 to inhale reagent position (not marked in figure).
Fill the filling that unit 20 completes sample, reagent.Fill on horizontal movement track and the sample disk 30 of unit 20 The reaction vessel position on reagent position, filling station 90 on sample bit, reagent disc 40 is intersected respectively, and point of intersection is respectively to inhale sample Position, inhale reagent position and filling position.In the present embodiment, filling unit is single sample charging mechanism, can do upper and lower and horizontal revolving motion, Not only sample had been filled but also had filled reagent, and can so make complete machine structure more compact lower with cost.It can also be integrated on filling unit 20 super The mixing mechanisms such as sonic generator, ultrasonic mixing is carried out to the reaction vessel after filling every time.In other embodiment, mixing machine Structure may be provided on filling station 90, be mixed for carrying out ultrasonic mixing or concussion to the reaction vessel after filling.
Reaction vessel is stored and provided to reaction vessel feed unit 70.In the present embodiment, in order to make complete machine more it is compact and Cost is lower, and reaction vessel feed unit uses advance arranged type.Reaction vessel feed unit 70 includes two reaction vessel supports Disk, sets a number of reaction vessel position on reaction vessel pallet, stores untapped reaction vessel.Reaction vessel supply is single In the range of the horizontal movement of buanch unit 50, such buanch unit 50 can travel through on pallet on each reaction vessel position member 70 Untapped reaction vessel, the test newly to start provides untapped reaction vessel.
Buanch unit 50 can be with horizontal movement, the transfer reaction container between the diverse location of automatic analysing apparatus 100.This In implementation, buanch unit 50 is arranged to 1, can do three-dimensional motion, can so make complete machine more compact lower with cost.Transfer is single Member 50 includes X to movable machinery arm 50b, Y-direction guide rail 50a, Y-direction movable machinery arm 50c and vertical movement mechanism and manipulator Refer to mechanisms such as (not marked in figure).Buanch unit 50 can be at the same time along X to, Y-direction horizontal moving mechanical finger, horizontal movement scope The scope in boundary polygon 56 is covered, can be by reaction vessel in reaction vessel feed unit 70, filling station 90, hatch unit 80 On reaction vessel position, the cleaning separation reaction vessel position 11a on processing unit 10, measurement reaction vessel position 11b, lose reaction and hold Shifted between device hole 60.
Reactant in 80 incubation reaction container of hatch unit.In the present embodiment, the attemperator of hatch unit 80 is pot Body 82 and upper cover (not marked in figure), rotating device are incubation disk 81.Having heaters and sensing on the inside of 82 side or bottom of pot body Device, surrounds the bottom and periphery for being incubated disk 81, provides constant-temperature incubation environment for hatch unit 80, prevents or reduce hatch unit 80 Heat scatters and disappears.The three circle reaction vessel positions using pivot as the center of circle can be provided with thereon around center axis rotation by being incubated disk 81 81a, 81b, 81c, certain number of turns can change, for example can be 1 circle, 2 circles, 4 circles or more etc..
Processing unit 10 carries out the reactant in reaction vessel cleaning separation and to reaction vessel independently of hatch unit Interior signal measures.In the present embodiment, the attemperator of processing unit 10 is pot body 12 and upper cover (not marked in figure), is located Reason disk is 11, cleaning separator is 16, measuring device 86.Having heaters and sensor on the inside of 12 side or bottom of pot body, The bottom and periphery of processing disk 11 are surrounded, isoperibol and dark room conditions is provided for processing unit 10, it is single to prevent or reduce incubation The lost and external stray light of first 10 heats influences.In addition to providing constant temperature and dark room conditions, pot body 12 is also supported and fixed The magnetic field generation device of separator 16 is cleaned, magnetic field environment is provided for cleaning separation.In the present embodiment, separator 16 is cleaned Magnet generation device be permanent magnet device, stronger and more stable magnetic field environment can be so provided.Clean separator 16 Flushing machine include liquid absorption device and priming device and mixing mechanism.Cleaning separator 16 can also coupled signal reagent add Mechanism is noted, all or part of signal reagent is filled to completing to clean in separated reaction vessel.Measuring device 86 includes faint light Detector photomultiplier (PMT), is directly installed in pot body 82, faint to being produced after addition signal reagent in reaction vessel Chemiluminescence signal measures.Two circles using pivot as the center of circle can be provided with thereon around center axis rotation by handling disk 11 Reaction vessel position 11a and 11b.Reaction vessel position wherein on inner ring 11a separates reaction vessel position to clean, on the 11b of outer ring Reaction vessel position is measurement reaction vessel position, so not only contributes to the installation of cleaning separator and measuring device, can also subtract The overall dimensions of few processing unit.Certainly, according to the needs of test, the number of turns for handling reaction vessel position on disk is to change , for example can be 1 circle, 3 circles, 4 circles or more etc..Hatch unit 80 be incubated end need to clean separated reaction vessel by Buanch unit 50 is transferred to the cleaning separation reaction vessel position on 11 inner ring 11a of processing disk, handles disc spins, transfers cleaning separation Reaction vessel on reaction vessel position carries out cleaning separation through over cleaning separator.Cleaning separation is completed to need the reaction measured Container, by buanch unit 50 from the measurement reaction vessel position on the cleaning separation bit transition to outer ring 11b on inner ring 11a, is handled Disc spins, reaction vessel to the measuring device transferred on measurement reaction vessel position measures, if the reactant of reaction vessel needs Want signal to be incubated, then realize that signal is incubated during transfer.
Below by taking one-step method is tested as an example, with reference to attached Figure 4 and 5, sketch automatic analysing apparatus 100 measurement procedure and Step.After test starts,
Step 200 loads reaction vessel:Buanch unit 50 is untapped anti-from the transfer of reaction vessel feed unit 70 one Container is answered to the reaction vessel position of filling station 90,
Step 201 fills sample and reagent:Fill unit 20 and draw sample and examination from suction sample bit and suction reagent position respectively Agent is filled into the reaction vessel on the reaction vessel position of filling station 90,
Step 202 mixes:If desired mix, then be integrated in the mixing mechanism of filling station 4 to the sample in reaction vessel and Reagent is mixed.If need not mix, the step is omitted,
Step 203 is incubated:The reaction vessel for having filled sample and reagent is transferred to incubation by buanch unit 50 from filling station Disk 81 3 encloses the reaction vessel position of wherein one circle of (81a, 81b, 81c), and reaction vessel starts to be incubated in hatch unit.Reaction is held Device is different because of specific test event in the incubation time of hatch unit 81, generally 5~60 minutes,
Step 204 cleaning separation:Reaction vessel, which is incubated, to be completed or is incubated after a certain period of time, and buanch unit 50 is by it from incubation The reaction vessel bit transition of unit 80 is to the cleaning separation reaction vessel position handled on 11 inner ring 11a of disk, and processing disk 11 is every solid Fix time and be rotationally advancing 1 position, transfer the reaction vessel on cleaning separation reaction vessel position to cleaning separator 16, pass through Clean the magnetic field of separator 16, complete imbibition to reaction vessel by the flushing machine and mixing mechanism of cleaning separator 16, Cleaning buffer solution, cleaning mixing are noted up to completing cleaning separation,
Step 205 fills signal reagent:After the completion of cleaning separation, processing disk 11 is transferred on cleaning separation reaction vessel position Reaction vessel leaves field region, from cleaning separator 16 on the signal reagent mechanism for filling liquid that couples injected into reaction vessel All or part of signal reagent.After all or part of signal reagent is completed in filling, buanch unit 50 is by reaction vessel from processing disk The measurement reaction vessel position on cleaning separation reaction vessel bit transition to outer ring 11b on 11 inner ring 11a,
Step 206 signal is incubated:If desired signal is incubated, and the measurement reaction vessel on the 11b of outer ring is transferred in processing disk 11 The reaction vessel of position is incubated to all or part of signal is completed during measuring device 86.If undesired signal is incubated, which saves Slightly,
Step 207 measures:When the reaction vessel for needing to measure transfers measured device 86, by measuring device 86 to reaction Reaction signal in container measures, and measurement result is sent to the control centre of automatic analysing apparatus after processing,
Step 208 abandons reaction vessel:Buanch unit 50 is by the reaction vessel after measurement from processing 11 outer ring 11b of disk Measurement reaction vessel bit transition is abandoned to reaction vessel hole 60 is abandoned.
Refer to the attached drawing 4 and attached drawing 6, delay one-step method testing process and step are step with one-step method examination difference 301-305, reagent dispenses and added in two times be once incubated, other steps are similar with one-step method, repeat no more.
Step 301 fills sample and the first reagent:Fill unit 20 and draw sample from suction sample bit and suction reagent position respectively It is filled into the first reagent in the reaction vessel on filling station 90,
Step 302 mixes:If desired mix, be then integrated in the vortex mixer of filling station 90 to the sample in reaction vessel and One reagent is mixed.If need not mix, the step is omitted,
Step 303 is incubated:The reaction vessel for having filled sample and the first reagent is transferred to by buanch unit 50 from filling station 4 The reaction vessel position that disk 81 3 encloses wherein one circle of (81a, 81b, 81c) is incubated, reaction vessel starts to be incubated in hatch unit.Instead Answer container different because of specific test event in the incubation time of hatch unit 81, generally 5~60 minutes,
Step 304 fills the second reagent:After being incubated for the first time, reaction vessel of the buanch unit 50 from hatch unit 80 Reaction vessel is again transferred to filling station 4 by position, and filling unit 20 is filled into filling station 90 from reagent position the second reagent of absorption is inhaled On reaction vessel in,
Step 305 mixes:If desired mix, be then integrated in the vortex mixer of filling station 90 to the sample in reaction vessel and One reagent is mixed.If need not mix, the step is omitted,
Refer to the attached drawing 4 and attached drawing 7, two-step method testing process and step are to add with delay one-step method examination difference Step 404, once cleaning separation is added:
Step 404 cleaning separation:Reaction vessel, which is incubated, to be completed or is incubated after a certain period of time, and buanch unit 50 is by it from incubation The reaction vessel bit transition of unit 80 is to the cleaning separation reaction vessel position handled on 11 inner ring 11a of disk, and processing disk 11 is every solid Fix time and be rotationally advancing 1 position, transfer the reaction vessel on cleaning separation reaction vessel position to cleaning separator 16, pass through Clean the magnetic field of separator 16, complete imbibition to reaction vessel by the flushing machine and mixing mechanism of cleaning separator 16, Note cleaning buffer solution, cleaning mixes until completion cleaning separation, after the completion of cleaning separates for the first time, buanch unit 50, which will react, to be held Device separates reaction vessel bit transition to filling station 90 from the cleaning on processing 11 inner ring 11a of disk.Unit 20 is filled from suction reagent position Draw in the reaction vessel that the second reagent is filled on filling station 90,
Other steps of two-step method are similar with delay one-step method, repeat no more.
It can be seen from the above descriptions that automatic analysing apparatus 100 uses mutually independent hatch unit and processing unit, it is incubated single First influence from processing unit, it is possible to achieve flexible incubation time, processing unit realize cleaning separation and measurement at the same time, no The independent cleaning separator disk and Measuring plate of prior art use are only eliminated, package size is reduced and reduces cost, also Simplify testing procedure and reduced the complexity and difficulty of control, avoid transfer of the reaction vessel between multiple disks.This Outside, processing unit is by setting different reaction vessel positions, and cleaning separator is arranged on the inside of processing unit, measuring device cloth Put on the outside of processing unit, separation will be cleaned and realized in the cleaning separation reaction vessel position of processing disk inner ring, measurement is being handled The measurement reaction vessel position of disk outer ring realizes, not only eliminates influencing each other for cleaning separation and measurement, it is single to also reduce processing The size of member, makes complete machine structure compacter, cost is lower, testing efficiency higher.
In addition to above-mentioned unique advantage, automatic analysis apparatus of the present invention can also flexibly expand and maximum limit The multiplexing of degree ground, realizes the seriation of product.On the basis of embodiment one, in order to further lift complete machine specifications parameter and test Flux, meets the terminal client demand of specimen amount bigger, can be by increasing buanch unit and filling element number, appropriate increase Hatch unit size increases the modes such as hatch unit quantity to realize.With reference to second that figure 8 is automatic analysing apparatus of the present invention Kind embodiment schematic diagram.Sample supply unit 30 takes the input mode of track and sample rack, can so accommodate more various This, can add sample in real time, and operation is also more convenient.Sample rack 32 and sample tube 31 thereon can be transported to the first filling Under the movement locus of unit 21.Reagent storage unit 40 adds reagent storage position, can place more reagent containers.Filling Unit 20 includes the first filling unit 21 and second and fills unit 22, and the first filling unit 21 fills sample, the second filling unit 22 filling reagents, the first filling unit 21 and second fill reaction vessel of the movement locus of unit 22 Jing Guo hatch unit 80 Position, so filling unit 20 can directly fill sample and reagent in the reaction vessel on 80 reaction vessel position of hatch unit, Filling station i.e. in the present embodiment is located in hatch unit.Certainly it can also increase more filling units, so improve sample-adding The speed of sheet and reagent.Pollution problem is carried in order to thoroughly solve sample, the first filling unit 21 is using once in the present embodiment Property the sample-adding of suction nozzle (Tip), 93 and 96 are respectively Tip and load positions and Tip unload positions in Fig. 8.Reaction vessel feed unit 70 in addition to it can provide new reaction vessel, can also provide new Tip heads.Buanch unit 50 includes independently doing three maintenance and operations Dynamic the first buanch unit 51 and the second buanch unit 52, the first buanch unit 51 mainly in reaction vessel feed unit 70, incubate Educate unit 80, Tip load between positions and Tip unloading positions, reaction vessel discarding hole 60a etc. positions transfer reaction container and Tip heads, the second buanch unit 52 mainly abandon hole in hatch unit 80, mixing station 90 and processing unit 10 and reaction vessel Transfer reaction container between 60b.Certainly, buanch unit can be 2 more than, can be arranged as required to more buanch units with Improve the efficiency and speed of reaction vessel transfer.The rotating device of hatch unit 80 sets two circle reaction vessel position 81a, 81b, place Unit 10 is managed independently of hatch unit, can multiplexing embodiment one entirely.At least one cleaning separation reaction handled on disk 11 is held In the range of movement of the second buanch unit 52, such second buanch unit 52 can for device position and at least one measurement reaction vessel position With between hatch unit, mixing station, processing unit and between cleaning separation reaction vessel position and measurement reaction vessel position turns Move reaction vessel.
Those skilled in the art are to be understood that the testing process of the present embodiment and step are led with embodiment one It is otherwise same to be:Sample and reagent is filled to be completed by the first and second filling unit cooperations;The filling of sample and reagent is all Completed on the reaction vessel position of hatch unit;The mixing for filling the reaction vessel after sample or reagent is complete by independent mixing station Into;Reaction vessel transfer is completed by the first and second buanch unit cooperations, and other actions and flow are identical with embodiment one Or it is similar, with reference to 5~Fig. 7 of figure, repeat no more.The embodiment compared with prior art, avoids extra large-sized cleaning Separator disk and Measuring plate, while divide the ruler for enclosing distribution and decreasing processing unit itself by the different reaction vessel position of function It is very little, so that complete machine is more compact, cost is lower, more efficient and better reliability.
The embodiment of the present invention additionally provides a kind of method of sample analysis, specifically includes:
Step is filled, sample and reagent are filled into reaction vessel;
Incubation step, is incubated the reaction vessel on the reaction vessel position of hatch unit;
Separating step is cleaned, the reactant in the reaction vessel on cleaning separation reaction vessel position to processing unit carries out Cleaning separation;
Measuring process, the reaction signal in the reaction vessel on measurement reaction vessel position to processing unit measure;
Transfer step, by buanch unit by the reaction vessel in the hatch unit and the processing unit and institute State and shifted between the cleaning separation reaction vessel position of processing unit, the measurement reaction vessel position of the processing unit.
The present invention realizes the incubation of reactant in reaction vessel centered on hatch unit, independently of the processing of hatch unit Unit carries out cleaning separation and to measurement reaction vessel position to the reactant in the reaction vessel on cleaning separation reaction vessel position On reaction vessel in signal measure, transfer of the reaction vessel between hatch unit and processing unit and processing are single The movement for being displaced through buanch unit between first differential responses container position is realized.The present invention not only increases reaction vessel transfer Reliability, eliminate individually cleaning separator disk and Measuring plate, simplified system structure and control flow, can also significantly reduce Processing unit size, makes hatch unit realize flexible incubation time.The present invention improves the work efficiency of analytical equipment, very well Solve the technical barriers such as current self-reacting device volume is big, detection speed is slow, of high cost, poor performance, not only saved experiment Room space, improves testing efficiency, and advantageously reduces expenses, mitigates testee's burden, has finally saved substantial amounts of Natural resources and social resources.
Technical characteristic or operating procedure described in the embodiment of the present invention can be combined in any suitable manner. Those skilled in the art are readily appreciated that, the order of step or action in the method for description of the embodiment of the present invention is can be with Change.Therefore, certain order is required unless otherwise indicated, and any order in attached drawing or detailed description is intended merely to Illustrative purpose, rather than necessary order.
It can include various steps in various embodiments of the present invention, these steps can be presented as can be by universal or special meter The instruction that the machine that calculation machine (or other electronic equipments) performs can perform.Alternatively, these steps can be by including to hold The hardware element of the particular logic circuit of these steps of row is performed or combined by hardware, software and/or firmware and performed.
Above by specific embodiment, the present invention is described, but the present invention is not limited to these specific implementations Example.It will be understood by those skilled in the art that various modifications, equivalent substitution, change etc. can also be done to the present invention, these conversion , all should be within protection scope of the present invention without departing from the spirit of the present invention.In addition, " the reality described in above many places Apply example " " the present embodiment " etc. represent different embodiments, naturally it is also possible to it is completely or partially combined in one embodiment.
Embodiment described above only expresses the several embodiments of the present invention, its description is more specific and detailed, but simultaneously Therefore the limitation to the scope of the claims of the present invention cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.

Claims (10)

  1. A kind of 1. automatic analysing apparatus, it is characterised in that:Including:
    Unit is filled, fills sample or/and reagent to reaction vessel;
    Hatch unit, the reactant in incubation reaction container;
    Processing unit, cleaning separate the reactant in reaction vessel and measure the reaction signal in reaction vessel;
    Buanch unit, between the different positions transfer reaction container;
    The hatch unit includes rotating device, sets incubation reaction container position on the rotating device, holds for incubation reaction Reactant in device;The processing unit includes processing disk, and cleaning separation reaction vessel position and measurement are set on the processing disk Reaction vessel position, the reaction signal being respectively used in the reactant and measurement reaction vessel in cleaning separation reaction vessel.
  2. 2. automatic analysing apparatus according to claim 1, it is characterised in that the buanch unit exists the reaction vessel Shifted between the hatch unit and the processing unit.
  3. 3. automatic analysing apparatus according to claim 1, it is characterised in that the buanch unit is also by the reaction vessel The cleaning separation reaction vessel position of the processing unit, the processing unit measurement reaction vessel position between shift.
  4. 4. automatic analysing apparatus according to claim 1, it is characterised in that the processing disk includes spiraling with the processing Turning at least two circle reaction vessel positions that center is the center of circle, the cleaning separation reaction vessel bit distribution at least one is enclosed wherein, The measurement reaction vessel bit distribution is on an at least other circle.
  5. 5. automatic analysing apparatus according to claim 4, it is characterised in that the processing disk includes spiraling with the processing Turn the inner ring and outer ring reaction vessel position that center is the center of circle, the cleaning separation reaction vessel bit distribution is on inner ring, the survey Quantitative response container bit distribution is on outer ring.
  6. 6. automatic analysing apparatus according to claim 1, it is characterised in that the reaction on the rotating device of the hatch unit is held Device bit distribution is at least two circles using the rotating device pivot as the center of circle.
  7. 7. automatic analysing apparatus according to claim 1, it is characterised in that the processing unit includes cleaning separator, right Reaction vessel on the cleaning separation reaction vessel position carries out cleaning separation, to remove the component being not associated with reactant.
  8. 8. automatic analysing apparatus according to claim 1, it is characterised in that the processing unit includes measuring device, the survey Measure device to be installed on the processing unit, the reaction signal in the reaction vessel on the measurement reaction vessel position is surveyed Amount.
  9. 9. automatic analysing apparatus according to claim 1, it is characterised in that:Measurement reaction vessel position on the processing disk Also there is signal incubation function.
  10. A kind of 10. method of sample analysis, it is characterised in that:Including
    Step is filled, sample and reagent are filled into reaction vessel;
    Incubation step, is incubated the reactant in the reaction vessel on the reaction vessel position of hatch unit;
    Separating step is cleaned, the reactant in the reaction vessel on cleaning separation reaction vessel position to processing unit cleans Separation;
    Measuring process, the reaction signal in the reaction vessel on measurement reaction vessel position to processing unit measure;
    Transfer step, by buanch unit by the reaction vessel at the hatch unit and the processing unit and the place Shifted between the cleaning separation reaction vessel position of reason unit, the measurement reaction vessel position of the processing unit.
CN201710979179.3A 2017-10-19 2017-10-19 Automatic analysing apparatus and its method of sample analysis Pending CN107942085A (en)

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WO2020087252A1 (en) * 2018-10-30 2020-05-07 深圳迎凯生物科技有限公司 Reaction device and immunity analyzer
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CN108444917A (en) * 2018-06-05 2018-08-24 深圳迎凯生物科技有限公司 Self-alignment Dim light measurement device
CN108732164A (en) * 2018-08-07 2018-11-02 广州万孚生物技术股份有限公司 Full-automatic chemiluminescence immunoassay analysis meter
CN108732164B (en) * 2018-08-07 2023-09-29 广州万孚生物技术股份有限公司 Full-automatic chemiluminescence immunoassay instrument
CN111122883B (en) * 2018-10-30 2023-08-08 深圳迎凯生物科技有限公司 Reaction device and immunoassay instrument
WO2020087252A1 (en) * 2018-10-30 2020-05-07 深圳迎凯生物科技有限公司 Reaction device and immunity analyzer
WO2020087238A1 (en) * 2018-10-30 2020-05-07 深圳迎凯生物科技有限公司 Reaction apparatus and immunoassay analyzer
CN111122883A (en) * 2018-10-30 2020-05-08 深圳迎凯生物科技有限公司 Reaction device and immunoassay analyzer
CN109030809A (en) * 2018-10-31 2018-12-18 蓝怡科技集团股份有限公司 A kind of anaphylactogen IgE antibody automatic analyzer
WO2020155386A1 (en) * 2019-02-02 2020-08-06 深圳迎凯生物科技有限公司 Liquid distribution method and immunoassay method
CN110261592A (en) * 2019-07-05 2019-09-20 杭州布封科技有限公司 A kind of analyte qualitativing quantitative measuring method
WO2021147188A1 (en) * 2020-01-21 2021-07-29 深圳迎凯生物科技有限公司 Reactor transfer method
CN113219194A (en) * 2020-01-21 2021-08-06 深圳迎凯生物科技有限公司 Analysis device
WO2021147186A1 (en) * 2020-01-21 2021-07-29 深圳迎凯生物科技有限公司 Dilution method and dilution apparatus
CN113219194B (en) * 2020-01-21 2023-10-20 深圳迎凯生物科技有限公司 Analysis device
CN113567690A (en) * 2020-04-28 2021-10-29 深圳迎凯生物科技有限公司 Incubation assembly, incubation device and automatic analysis device
CN113567691A (en) * 2020-04-28 2021-10-29 深圳迎凯生物科技有限公司 Incubation device and automatic analysis device

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