CN114019178A - Full-automatic immune biochemical integrated analyzer and use method thereof - Google Patents
Full-automatic immune biochemical integrated analyzer and use method thereof Download PDFInfo
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- CN114019178A CN114019178A CN202111313955.9A CN202111313955A CN114019178A CN 114019178 A CN114019178 A CN 114019178A CN 202111313955 A CN202111313955 A CN 202111313955A CN 114019178 A CN114019178 A CN 114019178A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00455—Controlling humidity in analyser
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00534—Mixing by a special element, e.g. stirrer
Abstract
The invention relates to the field of chemical analysis, and discloses a full-automatic immune biochemical integrated analyzer and a using method thereof. Carry out the sample through the sharing part and add, the immunity part carries out chemiluminescence immunodetection, and biochemical part carries out biochemical detection, can promote work efficiency, reduces and takes up an area of the space, can save purchasing cost.
Description
Technical Field
The invention relates to the field of chemical analysis, in particular to a full-automatic immune biochemical integrated analyzer and a using method thereof.
Background
The chemiluminescence immunoassay is an analyzer which is mainly used for measuring the components and the content of substances such as antigen and antibody contained in a sample by detecting the luminous intensity of a luminous reaction liquid corresponding to the sample to be measured. The biochemical analyzer is mainly an analyzer which mixes a sample to be tested and a corresponding reagent in a reaction cup, tests the colorimetric value of the mixed solution through chemical reaction, and determines indexes of various biochemical analysis items through calculation and analysis.
At present, immunological detection and biochemical detection are carried out by 2 instruments separately. The detection mechanism needs to buy two instruments, namely a biochemical analyzer and an immunoassay, separately to realize biochemical and immunological detection. For a primary hospital, two instruments are purchased at the same time, so that more requirements on the site and the purchase cost are bound to be met.
If the person to be detected needs to carry out immune and biochemical detection at the same time, at least 2 tubes of serum need to be collected, or manual sample separation is carried out to obtain at least two parts of serum, and the serum is respectively detected on an immune instrument and a biochemical instrument. Or the same sample is detected on an immunization instrument and a biochemical instrument in sequence. Therefore, the pain of the blood collected by the person to be detected is increased, the working efficiency is reduced, and the time of detection report is delayed.
For this reason, hospitals mainly purchase immune and biochemical test pipelines to solve the problem at present. But for the primary hospitals with small sample amount, the purchase of the immunization and biochemical test flow line occupies large area and has higher cost and low comprehensive cost performance.
Disclosure of Invention
The invention aims to provide a full-automatic immune biochemical integrated analyzer and a using method thereof, and aims to solve the problems that the existing equipment occupies a large space, is high in cost and reduces the production efficiency.
In order to achieve the above object, in a first aspect, the present invention provides a fully automatic immune biochemical integrated analyzer, including a shared portion, an immune portion and a biochemical portion, where the shared portion includes a sample reagent loading assembly and a dispensing assembly, and the dispensing assembly is slidably connected to the sample reagent loading assembly and located at one side of the sample reagent loading assembly; the immune part comprises an immune reaction disc assembly, an immune blending assembly, an immune photometry assembly, a reaction cup supply assembly, a magnetic separation cleaning assembly and a reaction cup transfer assembly, the immune reaction disc assembly is arranged on one side of the dispensing assembly, the immune blending assembly is arranged between the immune reaction disc assembly and the dispensing assembly, the immune photometry assembly is arranged on one side of the immune reaction disc assembly, the reaction cup supply assembly is arranged on one side of the immune blending assembly, the reaction cup transfer assembly is arranged on one side of the immune blending assembly close to the immune reaction disc assembly, the biochemical part comprises a biochemical reaction disc assembly, a biochemical stirring cleaning assembly, a biochemical cleaning hand assembly and a biochemical light path detection assembly, the reaction disc assembly is arranged on the outer side of the immune reaction disc assembly, biochemical stirring subassembly sets up the immunity light measuring subassembly is close to one side of branch injection subassembly, biochemical stirring washs the subassembly and sets up one side of biochemical stirring subassembly, biochemical washing hand subassembly sets up one side of biochemical reaction dish subassembly, and is close to immunity light measuring subassembly, biochemical light path detection subassembly sets up biochemical reaction dish subassembly is close to one side of reaction cup transfer subassembly, magnetic separation washs the subassembly and sets up divide the injection subassembly with biochemical stirring washs between the subassembly.
The immune part also comprises an immune cleaning component, and the immune cleaning component is arranged between the immune blending component and the dispensing component.
The dispensing assembly may be cleaned by the immuno-cleaning assembly.
And the magnetic separation cleaning component is used for cleaning and separating the combined substances and the free substances so as to prevent the free substances from participating in the reaction and influencing the photometric result.
Wherein, immunization portion still includes waste liquid recovery subassembly, waste liquid recovery subassembly sets up in biochemical reaction dish subassembly keeps away from one side of annotating the subassembly with water.
And recovering the waste liquid in the reaction cup and carrying the reaction cup through the waste liquid recovery assembly.
Wherein the immunization section further comprises a waste collection assembly disposed on one side of the waste recovery assembly.
The waste collecting assembly is used for collecting the used reaction cups to be processed in a centralized manner.
In a second aspect, the present invention further provides an immunoassay method for a full-automatic immunoassay and biochemical analysis integrated analyzer, including: the reaction cup transferring component transfers the reaction cups from the reaction cup supply component to the blending component;
the dispensing assembly sucks a sample and a reagent from the corresponding position of the sample reagent loading assembly and dispenses the sample and the reagent into a reaction cup;
the mixing component is used for mixing the reaction cups uniformly, and after the mixing time is reached, the reaction cup transfer component is used for transferring the reaction cups to the reaction plate component for constant-temperature incubation;
after incubation is finished, the reaction cup is transferred to a magnetic separation cleaning assembly, magnetic beads combined by the antibody and the antigen in the reaction cup are collected, and other impurities in the reaction cup are cleaned;
and after the washing is finished, performing substrate separate injection and uniform mixing on the reaction cup, and transferring the reaction cup to the reaction disk assembly by the reaction cup transfer assembly for incubation photometry.
In a third aspect, the present invention further provides a biochemical detection and use method of the full-automatic immune biochemical integrated analyzer, including: the biochemical reaction disc assembly rotates, the reaction cup stops being positioned at a reagent and sample adding position, and the reagent I and the sample are added into the reaction cup through the separate injection assembly;
the biochemical reaction disc component rotates, the reaction cup stops at the biochemical stirring component, and enters the reaction cup through a stirring rod of the biochemical stirring component to uniformly mix the solution;
incubating the uniformly mixed solution in the reaction cup in a constant-temperature heating ring, stopping the reaction cup at a reagent adding position after a preset period, and adding a second reagent into the reaction cup through a dispensing component if the second reagent needs to be added; if the reagent is not required to be added, no reagent is added;
the biochemical reaction disc component rotates, the reaction cup stops at the biochemical stirring component, and if a reagent II is added, the reagent II enters the reaction cup through a stirring rod of the biochemical stirring component to uniformly mix the solution; if not, not mixing;
after the light of the reaction cup is measured, the reaction disk rotates and stops for a plurality of cycles, and the reaction cup stops at a position from one needle to four needles of the biochemical cleaning hand assembly to clean the reaction cup;
the reaction cup is cleaned by one to four needles of the cleaning hand, and is rotated by a plurality of periods through the reaction disc, and stops at the sample adding position of the reagent and the sample, so that the next round of sample test can be carried out.
The invention relates to a full-automatic immune biochemical integrated analyzer and a using method thereof, wherein a shared part comprises a sample reagent loading assembly and a dispensing assembly, the immune part comprises an immune reaction disc assembly, an immune blending assembly, a magnetic separation cleaning assembly, an immune photometric assembly, a reaction cup supply assembly and a reaction cup transfer assembly, the immune reaction disc assembly is arranged on one side of the dispensing assembly, the immune blending assembly is arranged between the immune reaction disc assembly and the dispensing assembly, the immune photometric assembly is arranged on one side of the immune reaction disc assembly, the reaction cup supply assembly is arranged on one side of the immune blending assembly, and the reaction cup transfer assembly is arranged on one side of the immune blending assembly close to the immune reaction disc assembly. Biochemical part includes biochemical reaction dish subassembly, biochemical stirring and washs subassembly, biochemical washing hand subassembly and biochemical light path detection subassembly, the reaction dish subassembly sets up the immunoreaction dish subassembly outside, biochemical stirring subassembly sets up the immunity photometry subassembly is close to one side of branch injection subassembly, biochemical stirring washs the subassembly and sets up one side of biochemical stirring subassembly, biochemical washing hand subassembly sets up one side of biochemical reaction dish subassembly, and is close to immunity photometry subassembly, biochemical light path detection subassembly sets up biochemical reaction dish subassembly is close to one side of reaction cup transfer subassembly. So that the chemiluminescence immunoassay can be carried out, and the biochemical assay can also be carried out; the same tube of serum sample is used for one-time machine installation, so that the immune and biochemical detection can be carried out, and the working efficiency is improved; a non-immune and biochemical flow line reduces the use requirement on space; and the procurement budget is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a structural diagram of a fully automatic immuno-biochemical integrated analyzer according to the present invention;
FIG. 2 is a flow chart of the immunoassay method of the fully automatic immunoassay and biochemical analyzer according to the present invention;
FIG. 3 is a flow chart of the biochemical detection method of the fully automatic immune biochemical analyzer according to the present invention.
1-shared part, 2-immune part, 3-biochemical part, 11-sample reagent loading component, 12-dispensing component, 13-immune cleaning component, 14-biochemical cleaning component, 21-immune reaction disc component, 22-immune blending component, 23-immunity photometry component, 24-reaction cup supply component, 25-reaction cup transfer component, 26-magnetic separation cleaning component, 27-waste liquid recovery component, 28-waste material collection component, 31-biochemical reaction disc component, 32-biochemical stirring component, 33-biochemical stirring cleaning component, 34-biochemical cleaning hand component and 35-biochemical light path detection component.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In a first aspect, referring to fig. 1, the present invention provides a full-automatic immune biochemical analyzer, including:
the reagent kit comprises a common part 1, an immunization part 2 and a biochemical part 3, wherein the common part 1 comprises a sample reagent loading assembly 11 and a dispensing assembly 12, and the dispensing assembly 12 is connected with the sample reagent loading assembly 11 in a sliding mode and is positioned on one side of the sample reagent loading assembly 11;
immune part 2 includes immune response dish subassembly 21, immune mixing subassembly 22, immunity light subassembly 23, reaction cup supply assembly 24, magnetic separation washs subassembly 26 and reaction cup transfer assembly 25, immune response dish subassembly 21 sets up one side of dispensing subassembly 12, immune mixing subassembly 22 sets up immune response dish subassembly 21 with dispensing between the subassembly 12, immunity light subassembly 23 sets up one side of immune response dish subassembly 21, reaction cup supply assembly 24 sets up one side of immune mixing subassembly 22, reaction cup transfer assembly 25 sets up immune mixing subassembly 22 is close to one side of immune response dish subassembly 21, biochemical part 3 includes biochemical reaction dish subassembly 31, biochemical stirring subassembly 32, biochemical stirring washing subassembly 33, biochemical washing hand subassembly 34 and biochemical light path detection subassembly 35, the reaction disc assembly is arranged on the outer side of the immune reaction disc assembly 21, the biochemical stirring assembly 32 is arranged on one side, close to the dispensing assembly 12, of the immunity and light measuring assembly 23, the biochemical stirring and cleaning assembly 33 is arranged on one side of the biochemical stirring assembly 32, the biochemical hand cleaning assembly 34 is arranged on one side of the biochemical reaction disc assembly 31 and close to the immunity and light measuring assembly 23, the biochemical light path detection assembly 35 is arranged on one side, close to the reaction cup transfer assembly 25, of the biochemical reaction disc assembly 31, and the magnetic separation and cleaning assembly 26 is arranged between the dispensing assembly 12 and the biochemical stirring and cleaning assembly 33.
In the present embodiment, the reagent sample loading module is mainly composed of a sample loading tray and a reagent loading tray. The reagent loading tray mainly comprises a motor driving part, a rotatable tray body and a closed reagent bin, wherein the closed reagent bin is used for dividing a reagent loading area and an external environment, and refrigerating is carried out through a refrigerating and refrigerating part arranged on the reagent bin to jointly ensure the constant temperature of the reagent, and the rotatable tray body is positioned in the closed reagent bin and is used for loading a reagent box; the rotary disk body is compatible with the immunoassay kit and the biochemical kit. The immunoassay kit placed can realize the function of a uniform mixing magnetic bead reagent bin when the disk body is rotated. The sample loading disc is positioned on the outer ring of the reagent loading disc, the two disc bodies are coaxially arranged, the sample loading disc mainly comprises a motor driving part and a rotatable disc body, the rotatable disc body is used for loading a sample, a set of scanners is arranged on the outer side of the sample disc, and the sample loading disc and the reagent loading disc are provided with a notch at the same position on the circumference and used for respectively scanning the sample and the reagent and automatically identifying the type and the item of the reagent.
The separate injection assembly 12 is located above the sample reagent loading assembly 11 and mainly comprises a separate injection needle and an injection arm, the separate injection needle realizes the suction and separate injection operation of a sample and a reagent through an execution element such as a pump and a transmission storage element such as a needle injection pipeline, the injection arm is composed of an xy two-axis linkage driving device and can simultaneously realize the movement in the horizontal and vertical directions, a driving component is a motor, a transmission component can be a synchronous belt and a guide rail, a screw rod guide rail or a mechanical structure for realizing linear movement such as a gear rack.
The movement track of the dispensing component 12 comprises a reagent sucking position and a sample sucking position corresponding to a reagent sample disc, an immune cleaning component 13, a sample adding position of a blending component and a sample adding position of a biochemical reaction disc; quantitatively sucking at a sample position and a reagent position respectively, performing separate injection in a specified reaction cup on the blending component when performing immunoassay, and performing separate injection at a specified sample adding position of a biochemical reaction disc when performing biochemical assay. After the separated injection is finished, cleaning is carried out on the corresponding immune cleaning component 13 and biochemical cleaning component 14, so that cross contamination is avoided; the dispensing assembly 12 is positioned above the instrument to ensure that it does not interfere with other mechanisms during movement.
The reaction cups are supplied in an automatic loading arrangement mode, the assembly mainly comprises a hopper, a transportation assembly and an arrangement buffer channel, the hopper is of a box structure with an opening at the upper part, the design of the big-end-up ensures that the reaction cups can smoothly slide into the lower end without being clamped, the transportation assembly is arranged on the side of the hopper and moves from bottom to top in an escalator mode, the transportation end comprises a small grid, each grid can contain one reaction cup and is driven by a motor to realize circulation motion so as to ensure the continuous supply of the reaction cups, the arrangement buffer channel receives the reaction cups transferred from the low position to the high position by the transportation assembly and forcibly arranges the reaction cups on a straight line, after the reaction cups at the positioning position are used, one reaction cup is automatically supplied and positioned again so as to be used next time, the movement stroke of the reaction cup transfer assembly 25 can be reduced, and the requirement on positioning precision can be simplified, the component is directly arranged on the instrument bottom plate so as to achieve the positioning effect.
The immune blending component 22 mainly comprises a motor driving device, a synchronous belt (or gear) transmission component and a blending block movement component, wherein the blending block and the rotating shaft are designed eccentrically, and when the motor rotates, the blending block is driven to execute eccentric rotary motion, so that the vortex blending effect is achieved. The mixing subassembly can bear a plurality of reaction cups, once only carries out a plurality of reaction cup mixing, just so can make each reaction cup mixing time abundant, and the mixing effect is better.
Install in the inside heater block of heat preservation circle and heat the accuse temperature, guarantee the constancy temperature that the reaction cup was hatched jointly, but have a plurality of apertures in the vertical direction of rotatable disk body for the reaction cup is stored in the location, and the reaction cup can carry out rotary motion around the center pin along with the disk body, realizes the effect that can operate in the different reaction cups in same position. The assembly is integrally arranged on the instrument bottom plate so as to achieve the positioning effect.
The immunity light measuring component 23 is arranged in front of the immunity reaction disc component 21 and is far away from the biochemical light path detection component 35, so that the influence on the light measuring component caused by the luminescence of the biochemical light path is avoided. The device comprises a light path and an electric control component, performs light measurement in a disc, ensures that the light-shielding effect and the reaction environment are kept unchanged, is used for measuring an optical signal of a reaction cup which is added with a substrate and reacts stably, and measures and analyzes the content of a compound according to the measured optical signal.
The reaction cup transfer component 25 is composed of a three-dimensional motion platform and a cup grabbing execution element, the three-dimensional motion platform can realize three-axis linkage in space, and the cup grabbing execution element is matched with the three-dimensional motion platform, namely, the transfer of each point of the reaction cup is realized. The gripping cup executing element can judge various gripping states including failure of gripping the reaction cup, dropping of the reaction cup, interference of placing of the reaction cup and the like through the sensor.
The biochemical reaction disc component 31 is arranged at the same circle center position with the immunoreaction disc component 21 and is positioned at the outer ring of the immunoreaction disc component 21. Is independently controlled from the immunoreaction disc. A separate constant temperature heating system. Mainly comprises a motor driving device, a rotary disc body, a reaction cup bracket, a reaction cup and a constant temperature heating ring. The reaction disc is driven to rotate by the motor driving device. A plurality of reaction cup brackets are arranged on the rotary disc body, and each reaction cup bracket can be provided with a plurality of reaction cups. The reaction cup can be cleaned and reused. The added sample and reagents are added to the cuvette, which is incubated in a thermostated heating loop.
The biochemical stirring assembly 32 stops the stirring rod above the reaction cup mainly through an up-down rotating mechanism, and descends into the reaction cup, so that the stirring rod in the shape of a paddle enters the reagent sample mixed liquid. Meanwhile, the stirring rod is connected with the direct current motors respectively, and the stirring rod is driven to rotate through the rotation of the direct current motors, so that the stirring action is realized. This subassembly has two stirring rods, and the symmetry 180 degrees are placed, and a mixing reaction cup, another wash at biochemical stirring washing subassembly 33, so the alternate use stirring rod, raise the efficiency.
The biochemical stirring and cleaning assembly 33 mainly cleans the stirring rod of the biochemical stirring assembly 32. The cup is a cylindrical cup structure with a central ring, a liquid outlet is arranged in the middle, distilled water flows out in a fountain mode through a pipeline, and a stirring rod extends into the cup structure for cleaning. The distilled water flows out to a waste liquid pipe through the outer ring and is discharged.
The biochemical hand cleaning component 34 drives the belt through the motor to drive the cleaning head to move up and down. The cleaning head has 4 liquid suction needles, wherein the last needle is of an insert structure and three liquid spitting needles. The cleaning head moves downwards, four liquid sucking needles and two liquid spitting needles on the cleaning head respectively enter corresponding reaction cups, liquid suction is firstly carried out to discharge waste liquid, and distilled water is injected into the positions of the first two needles to clean the reaction cups. And only cleaning the insert block at the last needle, and sucking the residual liquid in the reaction cup. The reaction disc rotates according to regular period, the reaction cup advances one grid per period, thereby achieving four-stage cleaning effect of absorbing liquid four times and discharging liquid two times.
The biochemical light path detection component 35 is installed behind the biochemical reaction disc, and avoids interference with the immunity photometry component 23. The assembly illuminates the sample being tested in the cuvette with a stable light source and determines the neutral composition and concentration of the sample based on the absorption spectrum of the sample.
A magnetic separation cleaning assembly 26 is located on the front side of the instrument for cleaning and separating bound and free objects from the reaction of the free objects to affect the photometric results. The device comprises a storage component, a magnetic absorption component, a liquid injection component, a uniform mixing component, a substrate separate injection component and the like, wherein the storage component is of a disc structure and is provided with a plurality of small holes distributed in a circumferential array and used for storing reaction cups, the disc body can be controlled by a motor to rotate and move, each reaction cup is transferred to different positions, then liquid absorption, liquid injection, uniform mixing, magnetic absorption and other operations are carried out, the magnetic absorption component is distributed on the side surface of the storage component and used for carrying out magnetic field absorption on the reaction cups at a specified position to ensure that a combined object cannot be removed during cleaning and separation, the liquid absorption component comprises a liquid absorption needle and a lifting mechanism platform, the lifting mechanism platform can control the liquid absorption needle to descend into the reaction cups for absorbing liquid and then ascend and retreat from the reaction cups to avoid interference during rotation of the storage component, the liquid absorption needle is arranged on the lifting mechanism platform to perform absorption and cleaning actions on other substances except for combination, reach the separation effect, annotate the liquid part and be used for annotating the injection cleaning solution, it can be for fixing in the point position top of annotating, also can be for installing on the elevating system platform together with imbibition part, for the combination with the dissociate dispersion that does not wash the separation provides the environment, in order to wait to wash the separation once more, the mixing part is unanimous with above-mentioned mixing subassembly for mix the reaction cup who annotates the liquid, guarantee the combination and the abundant dispersion of dissociate, in order to wait to inhale the attraction once more and wash the separation.
Further, the immunization part 2 further comprises an immunization cleaning component 13, and the immunization cleaning component 13 is arranged between the immunization mixing component 22 and the dispensing component 12.
In the present embodiment, the immuno-cleaning module 13 mainly includes a cleaning reservoir and a cleaning solution supply member, and the cleaning solution supply member can be mounted at other positions, and therefore, not discussed, the cleaning reservoir is a hollow container having a liquid inlet hole and a liquid outlet hole, and the hollow portion can accommodate a certain amount of cleaning solution and has a slender structure similar to the shape of a dispensing needle, and the dispensing needle can perform a cleaning operation therein without using too much cleaning solution.
Further, the immunization part 2 further comprises a waste liquid recovery assembly 27, and the waste liquid recovery assembly 27 is arranged on the side of the biochemical reaction disc assembly 31 far away from the dispensing assembly 12; the immunization portion 2 further comprises a waste collection assembly 28, the waste collection assembly 28 being disposed on one side of the waste recovery assembly 27.
In this embodiment, the waste liquid recovery unit 27 is composed of a liquid suction needle, a cup grasping hand, and a lifting/lowering steering mechanism, and the liquid suction needle descends with the lifting/lowering mechanism to suck waste liquid into the reaction cup and discharge the waste liquid out of the apparatus through a pipeline. Then a reaction cup is grabbed by a cup grabbing hand and ascends along with the lifting mechanism, the rotating mechanism moves the reaction cup to an appointed position through a rotating arm, the assembly absorbs and discharges waste liquid of the reaction cup after optical measurement is finished, the reaction cup is discarded, and dry-wet separation is performed, so that biological pollution is avoided, the safety of an operator is effectively guaranteed, and meanwhile, the garbage disposal of a laboratory is facilitated; garbage collection subassembly 28 is top open-ended box structure for hold the reaction cup that uses the completion, be located the instrument side, through opening side door switch, load and unload, the simple operation.
Further, the common part 1 further comprises a biochemical cleaning assembly 14, and the biochemical cleaning assembly 14 is arranged between the dispensing assembly 12 and the immunological cleaning assembly 13.
In the present embodiment, the biochemical cleaning module 14 mainly includes a cleaning tank and a cleaning deionized water supply unit, and the deionized water supply unit can be mounted at other positions, and therefore, not shown, the cleaning tank is a hollow container having a liquid inlet hole and a liquid outlet hole, the middle hole has a slender structure, the dispensing needle extends into the middle hole to perform shower type outer wall cleaning, and the dispensing needle discharges deionized water to perform inner wall cleaning. All deionized water flows out from top to bottom and finally flows out from a liquid discharge hole at the lower part, and the action is finished.
In a second aspect, referring to fig. 2, the present invention further provides an immunoassay method of a full-automatic immune biochemical analyzer, including:
s101, the reaction cup transferring component 25 transfers the reaction cup from the reaction cup supply component 24 to the blending component;
s102, the dispensing assembly 12 sucks a certain amount of samples and reagents from the corresponding positions of the sample reagent loading assembly 11 and dispenses the samples and the reagents into the reaction cup;
s103, uniformly mixing the reaction cup by the uniformly mixing component, and transferring the reaction cup to the reaction disk component by the reaction cup transferring component 25 for constant-temperature incubation after the uniform mixing time is reached, so that the antibody/antigen is combined with the magnetic beads;
s104, after the incubation is finished, the reaction cup is transferred to a magnetic separation cleaning assembly, the magnetic beads combined by the antibody and the antigen in the reaction cup are collected, and other impurities in the reaction cup are cleaned.
S105, after cleaning, performing substrate separate injection and uniform mixing on the reaction cup, and transferring the reaction cup to the reaction disk assembly by the reaction cup transfer assembly for incubation photometry.
The test was carried out here using a one-step process. The reaction cup transfer assembly 25 transfers the reaction cup from the reaction cup supply assembly 24 to the blending assembly, the dispensing assembly 12 sucks a certain amount of sample and reagent from the corresponding position of the sample reagent loading assembly 11, the sample and the reagent are dispensed into the reaction cup, the blending assembly performs blending operation on the reaction cup, after the blending time is up, the reaction cup transfer assembly 25 transfers the reaction cup to the reaction disk assembly for incubation at a constant temperature, after the incubation time is up, the reaction cup transfer assembly 25 transfers the reaction cup from the reaction disk assembly to the magnetic separation cleaning assembly 26 for cleaning and separation, after the substrate dispensing and blending are finished, the reaction cup transfer assembly 25 transfers the reaction cup to the reaction disk assembly for incubation and photometry, after the incubation is finished, the solid-liquid separation is performed, the solid waste is collected and treated, and the liquid waste is uniformly treated and discharged.
In addition, the method can also adopt a two-step method for one-time separation. The specific operation is that the reaction cup transfer component 25 transfers the reaction cup from the reaction cup supply component 24 to the blending component, the dispensing component 12 sucks a certain amount of sample and reagent from the corresponding position of the sample reagent loading component 11 and dispenses the sample and reagent into the reaction cup, the blending component performs blending operation on the reaction cup, after the blending time is reached, the reaction cup transfer component 25 transfers the reaction cup into the reaction cup component for constant temperature incubation, after the incubation time is reached, the reaction cup transfer component 25 transfers the reaction cup from the reaction cup component to the blending component, the dispensing component 12 sucks a certain amount of reagent from the corresponding position of the sample reagent loading component 11 and dispenses the reagent into the reaction cup, the blending component performs blending operation on the reaction cup, after the blending time is reached, the reaction cup transfer component 25 transfers the reaction cup into the reaction cup component for constant temperature incubation, after the incubation time is reached, the reaction cup transfer assembly 25 transfers the reaction cup from the reaction disk assembly to the magnetic separation cleaning assembly 26 for cleaning and separation, substrate separate injection and uniform mixing are carried out after the reaction cup transfer assembly 25 transfers the reaction cup to the reaction disk assembly for incubation and photometric measurement, solid-liquid separation is carried out after the reaction cup transfer assembly is finished, solid waste materials are collected and treated respectively, and liquid waste liquid is treated and discharged uniformly.
Two-step separation may also be employed. The specific operation is that the reaction cup transfer component 25 transfers the reaction cup from the reaction cup supply component 24 to the blending component, the dispensing component 12 sucks a certain amount of sample and reagent from the corresponding position of the sample reagent loading component 11 and dispenses the sample and reagent into the reaction cup, the blending component performs blending operation on the reaction cup, after the blending time is up, the reaction cup transfer component 25 transfers the reaction cup into the reaction cup component for constant temperature incubation, after the incubation time is up, the reaction cup transfer component 25 transfers the reaction cup from the reaction cup component to the magnetic separation cleaning component 26 for cleaning and separation, the reaction cup transfer component 25 transfers the reaction cup into the blending component for dispensing, the reaction cup transfer component 12 sucks a certain amount of sample and reagent from the corresponding position of the sample reagent loading component 11 and dispenses the sample and reagent into the reaction cup, the blending component performs blending operation on the reaction cup, after the blending time is up, the reaction cup transfer component 25 transfers the reaction cup into the reaction cup component for constant temperature incubation, and when the incubation time is up, the reaction cup transfer assembly 25 transfers the reaction cup from the reaction disk assembly to the magnetic separation cleaning assembly 26 for cleaning and separation, substrate separate injection and uniform mixing are carried out after the reaction cup transfer assembly is finished, the reaction cup is transferred to the reaction disk assembly by the reaction cup transfer assembly 25 for incubation and photometric measurement, solid-liquid separation is carried out after the reaction cup transfer assembly is finished, solid waste materials are collected and treated respectively, and liquid waste liquid is treated and discharged uniformly.
In a third aspect, referring to fig. 3, the present invention further provides a biochemical detection and use method of a full-automatic immune biochemical integrated analyzer, including:
s201, the biochemical reaction disc assembly 31 rotates, the reaction cup stops being located at a reagent and sample adding position, and the reagent I and the sample are added into the reaction cup through the dispensing assembly 12;
s202, rotating the biochemical reaction disc component 31, stopping the reaction cup at the biochemical stirring component 32, entering the reaction cup through a stirring rod of the biochemical stirring component 32, and uniformly mixing the solution;
s203, incubating the uniformly mixed solution in the reaction cup in a constant-temperature heating ring, stopping the reaction cup at a reagent adding position after a preset period, and adding a second reagent into the reaction cup through the dispensing component 12 if the second reagent needs to be added; if the reagent is not required to be added, no reagent is added;
s204, the biochemical reaction disc component 31 rotates, the reaction cup is stopped at the biochemical stirring component 32, and if a reagent II is added, the reagent II enters the reaction cup through a stirring rod of the biochemical stirring component 32 to uniformly mix the solution; if not, not mixing;
s205 after the light of the reaction cup is measured, the reaction disk rotates and stops for a plurality of cycles, and the reaction cup stops at the position from one needle to four needles of the biochemical cleaning hand assembly 34 to clean the reaction cup;
s206 the reaction cup is cleaned by cleaning one to four needles, and is rotated for a plurality of periods through the reaction disc, and stops at the reagent and sample adding position, so that the next round of sample test can be carried out.
The invention adopts the layout of a full-automatic immune biochemical integrated analyzer, and can realize chemiluminescence immune detection and biochemical detection on the same instrument.
The kit comprises a common component part, an immunization component part and a biochemical component part. The common component parts comprise a sample reagent loading component 11 positioned on the right side of the instrument, a dispensing component 12 arranged above the sample reagent loading component 11, and an immune cleaning component 13 which is adjacent to the left side of the sample reagent loading component 11 and positioned on the motion trail of the dispensing component 12. The immunization part comprises an immunoreaction disc component 21 positioned on the left side of the instrument, an immune blending component 22 positioned between the immunoreaction disc component 21 and the immune cleaning component 13, an immunity light measuring component 23 arranged on the front side of the immunoreaction disc, a magnetic separation cleaning component 26 positioned on the front side of the instrument, a reaction cup supply component 24 for continuously supplying reaction cups, a waste material grabbing and sucking component for sucking waste liquid of the reaction cups after the light measurement and discarding the waste cups, a waste material collecting component positioned below the waste material grabbing and sucking component, and a reaction cup transferring component 25 positioned above all reaction cup transfer points. The biochemical part includes a biochemical reaction disk assembly 31 mounted concentrically on the immunoreaction disk. The biochemical stirring assembly 32 in front of the biochemical reaction disk and the biochemical stirring cleaning assembly 33 on the motion track thereof. A biochemical cleaning hand assembly 34 is positioned at the left front of the biochemical reaction disk. And the biochemical light path detection component 35 is positioned at the left rear part of the biochemical reaction disk.
Wherein the sample reagent loading assembly 11, the sample tray is on the outer ring of the reagent tray, and rotates independently. The reagent tray can be compatible with the placement of immunoreagents and biochemical reagents. The immunoreagent is placed in the reagent dish, when rotatory reagent dish, can realize the action of magnetic bead reagent storehouse mixing. The independent heat preservation structure of reagent dish refrigerates through cold-stored refrigeration part, guarantees reagent storage constant temperature. The sample dish of outer lane can realize the sample scanning, and a breach is left to the sample circle, can let the scanner scan the reagent in the reagent dish.
Wherein the immunoreaction disc component 21 and the biochemical reaction disc component 31 are arranged on the same circle center. The biochemical reaction disk is positioned on the outer ring of the immunoreaction disk. The two reaction disks separately and independently rotate and are respectively independent constant temperature systems, so that the temperature stability of respective reaction is ensured. Immunity photometric component 23 places in disk body the place in the place ahead, and biochemical photometric component places in the disk body rear side, and both are far away apart, avoid mutual interference.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
1. A full-automatic immune biochemical integrated analyzer, which is characterized in that,
the device comprises a shared part, an immune part and a biochemical part, wherein the shared part comprises a sample reagent loading assembly and a dispensing assembly, and the dispensing assembly is connected with the sample reagent loading assembly in a sliding manner and is positioned on one side of the sample reagent loading assembly;
the immune part comprises an immune reaction disc component, an immune blending component, an immune photometry component, a reaction cup supply component, a magnetic separation cleaning component and a reaction cup transfer component, wherein the immune reaction disc component is arranged on one side of the dispensing component, the immune blending component is arranged between the immune reaction disc component and the dispensing component, the immune photometry component is arranged on one side of the immune reaction disc component, the reaction cup supply component is arranged on one side of the immune blending component, and the reaction cup transfer component is arranged on one side of the immune blending component close to the immune reaction disc component;
biochemical part includes biochemical reaction dish subassembly, biochemical stirring washing subassembly, biochemical washing hand subassembly and biochemical light path detection subassembly, the reaction dish subassembly sets up the immunoreaction dish subassembly outside, biochemical stirring subassembly sets up the immunity photometry subassembly is close to one side of branch injection subassembly, biochemical stirring washing subassembly sets up one side of biochemical stirring subassembly, biochemical washing hand subassembly sets up one side of biochemical reaction dish subassembly, and is close to immunity photometry subassembly, biochemical light path detection subassembly sets up biochemical reaction dish subassembly is close to one side of reaction cup transfer subassembly, magnetic separation washing subassembly sets up divide the injection subassembly with biochemical stirring washs between the subassembly.
2. The fully automatic integrated immunoassay and biochemistry analyzer of claim 1,
the immune part also comprises an immune cleaning component, and the immune cleaning component is arranged between the immune blending component and the dispensing component.
3. The fully automatic integrated immunoassay and biochemistry analyzer of claim 1,
the immunization part further comprises a waste liquid recovery assembly, and the waste liquid recovery assembly is arranged on one side, far away from the separate injection assembly, of the biochemical reaction disc assembly.
4. The fully automatic immuno-biochemical integrated analyzer according to claim 3,
the immunization section further comprises a waste collection assembly disposed on one side of the waste recovery assembly.
5. An immunoassay method of a full-automatic immune biochemical integrated analyzer, which is applied to the full-automatic immune biochemical integrated analyzer according to any one of claims 1 to 4,
the method comprises the following steps: the reaction cup transferring component transfers the reaction cups from the reaction cup supply component to the blending component;
the dispensing assembly sucks a sample and a reagent from the corresponding position of the sample reagent loading assembly and dispenses the sample and the reagent into a reaction cup;
the mixing component is used for mixing the reaction cups uniformly, and after the mixing time is up, the reaction cup transfer component is used for transferring the reaction cups to the reaction plate component for constant-temperature incubation, so that the antibodies/antigens are combined with the magnetic beads;
after incubation is finished, the reaction cup is transferred to a magnetic separation cleaning assembly, magnetic beads combined by the antibody and the antigen in the reaction cup are collected, and other impurities in the reaction cup are cleaned;
and after the washing is finished, performing substrate separate injection and uniform mixing on the reaction cup, and transferring the reaction cup to the reaction disk assembly by the reaction cup transfer assembly for incubation photometry.
6. A biochemical detection using method of a full-automatic immune biochemical integrated analyzer, which is applied to the full-automatic immune biochemical integrated analyzer according to any one of claims 1 to 4, and is characterized by comprising the following steps:
the biochemical reaction disc assembly rotates, the reaction cup stops being positioned at a reagent and sample adding position, and the reagent I and the sample are added into the reaction cup through the separate injection assembly;
the biochemical reaction disc component rotates, the reaction cup stops at the biochemical stirring component, and enters the reaction cup through a stirring rod of the biochemical stirring component to uniformly mix the solution;
incubating the uniformly mixed solution in the reaction cup in a constant-temperature heating ring, stopping the reaction cup at a reagent adding position after a preset period, and adding a second reagent into the reaction cup through a dispensing component if the second reagent needs to be added; if the reagent is not required to be added, no reagent is added;
the biochemical reaction disc component rotates, the reaction cup stops at the biochemical stirring component, and if a reagent II is added, the reagent II enters the reaction cup through a stirring rod of the biochemical stirring component to uniformly mix the solution; if not, not mixing;
after the light of the reaction cup is measured, the reaction disk rotates and stops for a plurality of cycles, and the reaction cup stops at a position from one needle to four needles of the biochemical cleaning hand assembly to clean the reaction cup;
the reaction cup is cleaned by one to four needles of the cleaning hand, and is rotated by a plurality of periods through the reaction disc, and stops at the sample adding position of the reagent and the sample, so that the next round of sample test can be carried out.
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| CN202111313955.9A CN114019178A (en) | 2021-11-08 | 2021-11-08 | Full-automatic immune biochemical integrated analyzer and use method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114839392A (en) * | 2022-04-28 | 2022-08-02 | 科来思(深圳)科技有限公司 | Full-automatic chemiluminescence immunoassay analyzer |
| CN114895045A (en) * | 2022-04-28 | 2022-08-12 | 深圳市慧流体生物科技有限公司 | Full-automatic biochemical and immune integrated detection equipment |
| CN114965886A (en) * | 2022-05-18 | 2022-08-30 | 深圳无疆生命科学有限公司 | Sample adding disc device and sample analyzer |
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2021
- 2021-11-08 CN CN202111313955.9A patent/CN114019178A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114839392A (en) * | 2022-04-28 | 2022-08-02 | 科来思(深圳)科技有限公司 | Full-automatic chemiluminescence immunoassay analyzer |
| CN114895045A (en) * | 2022-04-28 | 2022-08-12 | 深圳市慧流体生物科技有限公司 | Full-automatic biochemical and immune integrated detection equipment |
| CN114965886A (en) * | 2022-05-18 | 2022-08-30 | 深圳无疆生命科学有限公司 | Sample adding disc device and sample analyzer |
| CN114965886B (en) * | 2022-05-18 | 2023-11-03 | 深圳无疆生命科学有限公司 | Sample loading tray device and sample analyzer |
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