CN117805357A - Sample analyzer and liquid injection control method - Google Patents
Sample analyzer and liquid injection control method Download PDFInfo
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- CN117805357A CN117805357A CN202211174619.5A CN202211174619A CN117805357A CN 117805357 A CN117805357 A CN 117805357A CN 202211174619 A CN202211174619 A CN 202211174619A CN 117805357 A CN117805357 A CN 117805357A
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- 239000007788 liquid Substances 0.000 title claims abstract description 491
- 238000002347 injection Methods 0.000 title claims abstract description 331
- 239000007924 injection Substances 0.000 title claims abstract description 331
- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000007246 mechanism Effects 0.000 claims abstract description 146
- 238000003860 storage Methods 0.000 claims abstract description 23
- 238000004140 cleaning Methods 0.000 claims description 115
- 238000006243 chemical reaction Methods 0.000 claims description 109
- 239000003153 chemical reaction reagent Substances 0.000 claims description 96
- 238000002156 mixing Methods 0.000 claims description 46
- 239000000758 substrate Substances 0.000 claims description 31
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- 239000012295 chemical reaction liquid Substances 0.000 claims description 13
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The embodiment of the application provides a sample analyzer and a liquid injection control method, wherein the sample analyzer comprises: a liquid container; the liquid injection mechanism is provided with a liquid injection port and is used for executing liquid injection to the liquid container through the liquid injection port, and comprises a liquid injection pipeline, a liquid storage container, a power component and a temperature control component, wherein the liquid storage container is used for storing liquid, the temperature control component is communicated between the liquid injection port and the liquid storage container, and the power component is communicated with the liquid injection port through the liquid injection pipeline and is used for providing power for the flowing of the liquid in the liquid injection pipeline; the temperature control component is used for adjusting the temperature of the liquid flowing through the liquid injection pipeline; and a controller for: when the liquid injection mechanism is in a non-working state, the power assembly is controlled to suck back air through the liquid injection port, so that liquid in the liquid injection pipeline at least partially flows back to the temperature control assembly, and the non-working state is a state that the liquid injection mechanism does not execute liquid injection operation.
Description
Technical Field
The application relates to the technical field of medical instruments, in particular to a sample analyzer and a liquid injection control method.
Background
The sample analyzer is used for analyzing a specific sample and obtaining a corresponding sample analysis result, and is widely applied to clinical examination. In a sample analysis process performed by a sample analyzer, it is generally required to perform a liquid injection operation on a target object by using a liquid injection mechanism, for example, a liquid container is described by using the target object as a liquid container, a solution injected into the liquid container by the liquid injection mechanism is a sample and/or a reagent to prepare a sample to be measured, and a cleaning agent is injected into the liquid container by the liquid injection mechanism to clean the liquid container or perform a cleaning operation on the liquid container.
However, in some specific item detection processes, when the solution is injected into the target object by the injection mechanism, the accuracy of the final sample detection is affected due to the large temperature fluctuation of the injected solution.
Disclosure of Invention
The embodiment of the application mainly aims at providing a sample analyzer and a liquid injection control method, aiming at improving the stability of the liquid injection temperature of a liquid injection mechanism in the process of executing the liquid injection operation of the liquid injection mechanism, and further improving the accuracy of a sample detection result.
In a first aspect, embodiments of the present application provide a sample analyzer, comprising:
a liquid container;
the liquid injection mechanism is provided with a liquid injection port and is used for executing liquid injection to the liquid container through the liquid injection port, and comprises a liquid injection pipeline, a liquid storage container, a power component and a temperature control component, wherein the liquid storage container is used for storing liquid, the temperature control component is communicated between the liquid injection port and the liquid storage container, and the power component is communicated with the liquid injection port through the liquid injection pipeline and is used for providing power for the flowing of the liquid in the liquid injection pipeline; the temperature control component is at least used for adjusting the temperature of the liquid flowing through the liquid injection pipeline; and
The controller is connected with the liquid injection mechanism and is used for:
and controlling the power assembly to suck back air through the liquid injection port when the liquid injection mechanism is in a non-working state, so that liquid in the liquid injection pipeline at least partially flows back to the temperature control assembly, wherein the non-working state is a state that the liquid injection mechanism does not execute liquid injection operation.
In a second aspect, embodiments of the present application further provide a method for controlling liquid injection, applied to a sample analyzer, the method including: and controlling the power assembly of the liquid injection mechanism to suck back air through the liquid injection port of the liquid injection mechanism when the liquid injection mechanism of the sample analyzer is in a non-working state, so that liquid in the liquid injection pipeline of the liquid injection mechanism at least partially flows back to the temperature control assembly of the liquid injection mechanism, wherein the non-working state is a state that the liquid injection mechanism does not execute liquid injection operation.
The embodiment of the application provides a sample analyzer and a liquid injection control method, in an implementation manner, the sample analyzer comprises: a liquid container; the liquid injection mechanism is provided with a liquid injection port and is used for executing liquid injection to the liquid container through the liquid injection port, and comprises a liquid injection pipeline, a liquid storage container, a power component and a temperature control component, wherein the liquid storage container is used for storing liquid, the temperature control component is communicated between the liquid injection port and the liquid storage container, and the power component is communicated with the liquid injection port through the liquid injection pipeline and is used for providing power for the flowing of the liquid in the liquid injection pipeline; the temperature control component is at least used for adjusting the temperature of the liquid flowing through the liquid injection pipeline; and the controller is connected with the liquid injection mechanism and is used for: and controlling the power assembly to suck back air through the liquid injection port when the liquid injection mechanism is in a non-working state, so that liquid in the liquid injection pipeline at least partially flows back to the temperature control assembly, wherein the non-working state is a state that the liquid injection mechanism does not execute liquid injection operation. In this embodiment of the application, through the control annotate liquid mechanism back suction air under non-operating condition to make annotate liquid in liquid injection pipeline of liquid injection mechanism at least part backward flow extremely the control by temperature change subassembly, thereby utilize the control by temperature change subassembly to preheat the liquid in the pipeline once more, when annotating liquid mechanism and carrying out annotating liquid operation, avoid to liquid container exit temperature lower liquid, and then realize annotating liquid mechanism and carry out annotating liquid operation in-process, to the solution temperature that liquid container the exit comparatively stable and more be close to target temperature, effectively promote sample testing result's accuracy.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure of embodiments of the present application.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a block diagram of components of a sample analyzer in one embodiment;
FIG. 2 is a schematic diagram of components of a sample analyzer in one embodiment;
FIG. 3 is a schematic diagram of components of a dispensing device of a sample analyzer in one embodiment;
FIG. 4 is a block diagram of the components of a separation and washing apparatus of a sample analyzer in one embodiment;
FIG. 5 is a schematic view of a liquid path structure of a first liquid injection mechanism of the separation and cleaning device according to an embodiment;
FIG. 6 is a schematic view of a liquid path structure of a second liquid injection mechanism of the cleaning device according to an embodiment;
fig. 7 is a test schematic of an immunoassay.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the described embodiments are some, but not all, examples of the present application. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.
In the description of the present application, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art in a specific context.
The flow diagrams depicted in the figures are merely illustrative and not necessarily all of the elements and operations/steps are included or performed in the order described. For example, some operations/steps may be further divided, combined, or partially combined, so that the order of actual execution may be changed according to actual situations.
Some embodiments of the present application will be described in detail below with reference to the accompanying drawings, and the features of the following examples and embodiments may be combined with each other without conflict.
Referring to fig. 1, a sample analyzer 100 is provided for analyzing a sample to be tested to obtain a corresponding analysis result.
In some embodiments, the sample analyzer includes, but is not limited to, at least one of: biochemical analyzer, immunity analyzer, coagulation analyzer, urine analyzer.
As shown in fig. 1, the sample analyzer 100 includes a dispensing device 10, a sample supply device 20, a reagent supply device 30, a reaction device 40, a mixing device 50, a detection device 60, and a controller 70.
The sample supply device 20 is used for providing a sample to be tested, the reagent supply device 30 is used for providing a reagent reacting with the sample, and the reaction device 40 is provided with at least one placing position for placing a liquid container, wherein the liquid container comprises, but is not limited to, a reaction cup. For convenience of description, in the embodiments of the present application, a liquid container is taken as an example of a reaction cup. The dispensing device 10 is used for injecting a sample supplied from the sample supply device 20 and a reagent supplied from the reagent supply device 30 into a reaction cup so that the sample and the reagent are mixed to form a reaction solution. The mixing device 50 is provided with a mixing position and is used for mixing the sample and the reagent placed in the reaction cup of the mixing position uniformly so that the sample and the reagent fully react to form a reaction solution. The detecting device 60 is used for detecting the reaction liquid in the reaction cup to obtain a corresponding detection result.
Referring to fig. 2-3, in some embodiments, the sample supply 20 may include a sample distribution module (SDM, sample Delivery Module) and a front end rail; the sample supply 20 may also be a sample tray comprising a plurality of sample locations where sample, such as sample tubes, may be placed, the sample tray being capable of dispensing samples to corresponding locations, such as to the point where the sample is aspirated by the dispensing device 10, by rotating its tray assembly. The dispensing device 10 is used to aspirate and discharge a sample into a cuvette 4011 to be loaded.
In some embodiments, the dispensing device 10 includes a sample dispensing device 10a, wherein the sample dispensing device 10a is configured to aspirate a sample supplied by the sample supply device 20 and transfer the sample to a predetermined location, such as to discharge to a cuvette 4011 to be loaded. The sample dispensing device 10a includes a sample needle 101, a first drive assembly 102, and a first pipetting drive unit 103, wherein the first drive assembly 102 is configured to support the sample needle 101 and drive the sample needle 101 to move. For example, the sample needle 101 is spatially moved in two or three dimensions by a two or three dimensional first drive assembly 102 so that the sample needle 101 can be moved to aspirate a sample carried by the sample supply 20.
The first pipetting driving unit 103 is configured to quantitatively aspirate a sample, for example, a blood sample to be measured by the sample needle 101, wherein the sample needle 101 is driven by the first driving component 102 to move into a sample tube loaded with the blood sample on the sample supplying device 20, aspirate the blood sample to be measured by the driving of the first pipetting driving unit 103, and convey the blood sample to be measured into the reaction cup 4011 of the reaction device 40, so that the blood sample to be measured aspirated by the dispensing device 10 and a reagent provided by the reagent supplying device 20 are mixed in the reaction cup 4011 to prepare a sample to be measured.
As shown in fig. 3, in some embodiments, the first driving assembly 102 includes a support frame 1021, the support frame 1021 is fixed on the support rod 1022, the support rod 1022 can move vertically and rotate, and the support frame 1021 is driven by the support rod 1022 to realize vertical movement and horizontal rotation. The sample needle 101 is disposed on the support 1021, and driven by the support 1021, can reach a target position. The first drive assembly 102 also includes a driver 1023 for driving the movement of the support pole 1022, such as, but not limited to, a stepper motor. Alternatively, the sample needle 101 may be detachably connected to the first drive assembly 102, or may be fixedly connected.
In some embodiments, the first pipetting drive unit 103 includes a line 1031 and a power assembly 1033, wherein the line 1031 is used for transporting the fluid medium, one end of the line 1031 is communicated with the sample needle 101, and the other end is communicated with the power assembly 1033, so that the flow direction of the fluid medium in the line 1031 is changed under the action of the power assembly 1033, so that the sample needle 101 can transfer the sample.
In some embodiments, the dispensing device 10 includes a reagent dispensing device 10b, the reagent supplying device 30 includes a reagent carrying member 301 for carrying a reagent, and the reagent dispensing device 10b of the dispensing device 10 sucks the reagent carried by the reagent supplying device 30 and supplies the reagent to the reaction device 40, wherein the reagent includes but is not limited to a chromogenic reagent, a diluent, a substrate solution, an enzyme-labeled reagent, and the like.
In some embodiments, the reagent carrier 301 may be a reagent disk, which is provided as a disk-shaped assembly having a plurality of positions for carrying reagent containers, and the reagent carrier 301 is capable of rotating and driving the reagent containers carried thereby to rotate the reagent containers to a specific position, such as a position where reagent is sucked by the reagent dispensing device 10 b. Wherein the number of reagent carrying members 301 may be one or more.
In some embodiments, the reagent dispensing device 10b may include a reagent needle, a second drive assembly, and a second pipetting drive unit. The reagent needle performs a two-dimensional or three-dimensional motion in space by the two-dimensional or three-dimensional second driving assembly, so that the reagent needle can move and cooperate with the second pipetting drive part to suck the reagent carried by the reagent carrying member 301 and move to the cuvette 4011 to be filled with the reagent and discharge the reagent to the cuvette 4011.
In some embodiments, the second driving component and the first driving component 102 have the same components, which are not described herein.
In some embodiments, the second pipetting drive unit and the first pipetting drive unit 103 have the same components, which are not described here.
In some embodiments, the reagent dispensing device 10b does not add reagent by means of a reagent needle, but adds reagent in a reagent tube to the cuvette 4011 by means of a dedicated line. In such embodiments, only sample needle 101 is provided, without a reagent needle.
It will be appreciated that depending on the body fluid to be tested and the item to be tested, the sample and reagent may be added in different ways, for example, both the sample and reagent may be added using the sample needle 101, or the sample may be added using the sample needle 101, the reagent may be added using the reagent needle, or only the sample may be added using the sample needle 101, or the reagent may be added using other means. That is, the sample dispensing device 10a of the dispensing device 10 is used for both transferring a sample and transferring a reagent; or the sample dispensing device 10a of the dispensing device 10 is used for transferring a sample, and the reagent dispensing device 10b is used for transferring a reagent; or the sample dispensing device 10a of the dispensing device 10 is used for transferring a sample, and the reagent is connected to a reagent container for holding the reagent through a dedicated line so as to be added to the cuvette 4011. Thus, the sample needle 101 and/or the reagent needle are also referred to as pipetting needle, i.e. the pipetting needle comprises at least either of the sample needle 101 and the reagent needle.
In some embodiments, the reaction device 40 has a support 401, and the support 401 has at least one placement site for placing a reaction cup 4011, the reaction cup 4011 is for receiving a sample supplied by the sample supply device 20 and a reagent supplied by the reagent supply device 30 and providing a reaction site for the sample and the reagent to mix to form a reaction liquid, and the reaction device 40 is also for incubating the reaction liquid within the reaction cup 4011.
For example, the support 401 of the reaction device 40 may be a reaction disk, as shown in fig. 2, which is configured as a disk-shaped component with one or more placement sites for placing reaction cups 4011, and the reaction disk can rotate and drive the reaction cups 4011 in the placement sites to rotate for distributing the reaction cups 4011 and incubating the reaction liquid in the reaction cups 4011 in the reaction disk.
It will be appreciated that the placement location for the liquid container may be provided not only on the reaction tray of the reaction device 40, but also independently of the reaction tray arrangement of the reaction device 40, the placement location being provided independently of the reaction tray 4 meaning that the placement location does not interfere with the rotation of the reaction tray itself. Alternatively, when the mixing device 50 does not perform the mixing operation, the mixing bits are multiplexed as the placement bits of the cuvette.
In some embodiments, the detection device 60 is used for measuring the reaction liquid in the reaction cup or the sample to be tested incubated by the reaction liquid, so as to obtain the reaction data of the sample. For example, the detection device 60 includes a photometry mechanism for detecting the luminous intensity of a sample to be measured, and calculates the concentration of the component to be measured in the sample, etc. by a calibration curve. Alternatively, the detecting device 60 is separately provided on the peripheral side of the reaction device 40.
In further embodiments, the detection device 60 includes an electrical detection mechanism (e.g., an impedance measurement mechanism) or other principle detection mechanism (e.g., an imaging measurement mechanism).
In some embodiments, the sample analyzer further comprises a dispatching device (not shown) for performing the dispatching of the reaction cup, for example, the dispatching device may grab the target object and drive the target object to move in a two-dimensional or three-dimensional space, so as to achieve the dispatching of the target object. For example, the scheduler may schedule the cuvette 4011 to a sample site in the sample analyzer so that the sample dispensing device 10a performs a sample filling operation on the cuvette 4011 placed in the sample site, or may schedule the cuvette 4011 to a reagent site in the sample analyzer so that the reagent dispensing device 10b performs a reagent filling operation on the cuvette 4011 placed in the reagent site, or may schedule the cuvette 4011 filled with the sample and the reagent to a mixing site of the mixing device 50 so that the solution in the cuvette 4011 is mixed at the mixing site, or may schedule the cuvette 4011 having completed the mixing operation on the mixing site of the mixing device 50 to the reaction device 40 for incubation.
In some embodiments, the sample analyzer 100 further includes a separation and cleaning device 80, where the separation and cleaning device 80 is used for performing magnetic separation and cleaning on the reaction solution, so as to extract the magnetic particles enriched with the analyte in the reaction solution, clean the impurities attached to the surface of the magnetic particles, improve the purity of the analyte in the sample to be tested, and further improve the accuracy of the sample detection result.
Referring to fig. 4, the separation cleaning device 80 illustratively includes a magnetic separation disc 801, an adsorption assembly 802, a first liquid injection mechanism 81, and a liquid suction assembly 803, wherein the magnetic separation disc 801 is provided with at least one magnetic separation site, and the magnetic separation disc 801 is a rotatable structure and is provided with at least one liquid injection site and at least one liquid suction site along its rotation path; the adsorption component 802 is provided with at least one magnetic adsorption component capable of adsorbing magnetic particles in the reaction liquid in the reaction cup at the magnetic separation position; the first liquid injection mechanism 81 is used for injecting a first cleaning liquid into the reaction cup which moves to the liquid injection level so as to clean impurities attached to the surfaces of the magnetic particles; the pipetting assembly 803 is used to perform pipetting operations on a cuvette that is moved to a pipetting position.
Specifically, after the separation cleaning device 80 receives the cuvette 4011, performing N-order magnetic separation cleaning on the cuvette 4011, wherein N is an integer greater than or equal to 1; for any one order of magnetic separation cleaning, it includes: after the reaction cup 4011 reaches the liquid injection level, the first liquid injection mechanism 81 adds a first cleaning liquid into the reaction cup 4011, performs magnetic separation cleaning on the reaction liquid in the reaction cup 4011, transfers the reaction cup 4011 after the magnetic separation cleaning to a liquid suction position, performs liquid suction on the reaction cup 4011 through the liquid suction component 803 to complete the magnetic separation cleaning of the first stage, and circulates in this way until the reaction cup 4011 after the magnetic separation cleaning of the N stages waits for dispatching the magnetic separation disc 801 of the separation cleaning device 80.
In some embodiments, the sample analyzer 100 further comprises a substrate injection device for adding a substrate to the cuvette that has completed the magnetic separation wash, e.g., the substrate injection device injects a substrate into the cuvette 4011 after the pipetting assembly 803 has completed the N-up pipetting operation for the cuvette 4011 that is located at the pipetting level.
Illustratively, after the reaction cup 4011 carrying the reaction solution formed by mixing the sample and the reagent is transferred to the reaction device 40, the reaction device 40 incubates the sample and the reagent in the reaction cup 4011, the incubated reaction cup 4011 is transferred to the separation and cleaning device 80 for magnetic separation and cleaning, the reaction cup 4011 after the magnetic separation and cleaning is injected with the substrate and transferred to the reaction device 40 for incubation, and the incubated reaction cup 4011 is transferred to the corresponding photometric position to enable the detection device 60 to perform luminescence detection on the object to be detected, so as to obtain the corresponding parameters of the sample. The transfer of cuvette 4011 may be performed by a scheduler within sample analyzer 100.
To facilitate understanding of the sample and reagent stage names, the sample and reagent stage names are described in detail herein: the sample in the cuvette 4011 is mixed with the reagent and then referred to as a mixture, also referred to as a reaction solution, and the reaction device 40 is capable of incubating the mixture in the cuvette 4011 to allow the sample to react with the reagent sufficiently, and at this time, substances in the cuvette 4011 are an analyte and impurities. The mixture is a substance formed by mixing a sample with a reagent, and is referred to as a mixture herein regardless of the ratio or concentration of the sample to the reagent. The incubated mixture is presented as the analyte and impurities in the cuvette 4011. The impurities may be insufficiently reacted substances, side reaction products generated by side reactions, other substances affecting the detection by the detection device 60, or the like, or a combination of at least two of the above. The separation and cleaning device 80 cleans the object to be measured and impurities in the reaction cup 4011 to remove the impurities in the reaction cup 4011 and retain the object to be measured in the reaction cup 4011. The detection device 60 can detect the object to be detected in the reaction cup 4011 to obtain various parameters of the sample. When a substrate is added to the cuvette 4011 after separation and washing, the substrate is mixed with the sample in the cuvette 4011, and the substrate does not change the property of the sample, but only increases the luminescence value of the sample, so that the substrate is still called the sample after being mixed with the sample.
Referring to fig. 5, in some embodiments, the first filling mechanism 81 is provided with a first filling port 8011, and is configured to perform filling to a liquid container through the first filling port 8011, where the liquid container includes, but is not limited to, a reaction cup.
Specifically, the first injection mechanism 81 includes a first injection pipe 811, a first storage container 812, a first power component 813, and a first temperature control component 814, where the first storage container 812 is used for storing a liquid (e.g., a first cleaning solution), the first temperature control component 814 is communicated between the first injection port 8011 and the first storage container 812, the first power component 813 is communicated with the first injection port 8011 through the first injection pipe 811, and is used for providing power for the liquid flowing in the first injection pipe 811, and the first temperature control component 814 is at least used for adjusting the temperature of the liquid flowing through the first injection pipe 811.
For example, in the process of injecting the cleaning liquid into the reaction cup 4011 where the magnetic separation is completed, the first liquid injection mechanism 81 transfers the first cleaning liquid stored in the storage liquid to the first temperature control assembly 814 through the first power assembly 813 to be preheated to a preset temperature, and transfers the first cleaning liquid preset to the preset temperature into the reaction cup 4011 located in the magnetic separation position through the first liquid injection pipeline 811 to clean impurities adhering to the surfaces of the magnetic particles in the reaction cup 4011.
It will be appreciated that the first temperature control assembly 814 is provided with a liquid circulation component that is in communication with the first liquid injection pipeline 811, and a preheating component that is used for preheating the liquid circulated in the liquid circulation component, where the liquid circulation component may be integrally formed with the first liquid injection pipeline 811, or may be separately formed with the first liquid injection pipeline 811 and then connected by a connecting piece or by welding, gluing, etc., and the preset component may directly heat the liquid circulation component, or may heat the heat conducting medium, and then indirectly heat the liquid circulation component by the heat conducting medium.
For example, the first liquid injection pipe 811 and the liquid circulation member are integrally formed, the liquid circulation member is wound around the heat transfer medium, and the preset member of the first temperature control unit 814 heats the heat transfer medium, so that the heat transfer medium is used to heat the liquid in the liquid circulation member. The temperature of the liquid in the liquid circulation member may be collected by a temperature sensor, for example, a temperature sensor is provided on an outer wall of the liquid circulation member to collect the temperature of the liquid in the liquid circulation member.
In some embodiments, the first power assembly 813 is connected to the first liquid storage container 812 and the first temperature control assembly 814 through a pipeline, and the first power assembly 813 includes a switching unit disposed on the pipeline for controlling on-off of the pipeline and a power unit for driving the cleaning liquid to flow in a preset direction in the pipeline. The control device 70 is communicatively connected to a switch unit including but not limited to a solenoid valve and/or a throttle valve and a power unit including but not limited to a pump, a syringe to control the power unit to supply cleaning liquid to the cuvette 4011 through the first filling port 8011 when filling the liquid container.
In some embodiments, the first injection mechanism 81 further includes an injection needle 810 communicating with the first injection line 811, and the first injection port 8011 is provided at an end of the injection needle 810, and the first injection mechanism 81 performs injection of the liquid into the liquid container through the injection needle 810. Alternatively, the port of the first filling line 811 is used as the first filling port 8011, and filling is directly performed into the liquid container through the first filling line 811.
Referring to fig. 6, in some embodiments, the sample analyzer 100 further includes a cleaning device 90, which includes a cleaning tank 901 and a second liquid injection mechanism 91, wherein the cleaning tank 901 is used as a liquid container for providing a cleaning place to clean target devices located in the cleaning tank 901, including but not limited to, a sample needle 101 and a reagent needle, by the cleaning liquid when the second liquid injection mechanism 91 injects the cleaning liquid into the cleaning tank.
Specifically, the second liquid injection mechanism 91 is provided with a second liquid injection port 9011, and the second liquid injection port 9011 of the second liquid injection mechanism 91 is located on the inner wall of the cleaning tank 901, so that liquid is injected into the cleaning tank 901 through the second liquid injection port 9011, and thereby a target device is cleaned in the cleaning tank 901, and waste liquid generated in the cleaning process of the target device can be discharged into a waste liquid container through a liquid discharge port 9012 of the cleaning tank 901.
In some embodiments, the second liquid injection mechanism 91 and the first liquid injection mechanism 81 have the same structure, that is, the second liquid injection mechanism 91 includes a second liquid injection pipeline 911, a second liquid storage container 912, a second power component 913, and a second temperature control component 914, where the second liquid storage container 912 stores a second cleaning liquid, the second temperature control component 914 is communicated between the second liquid injection port 9011 and the second liquid storage container 912, and the second power component 913 is communicated with the second liquid injection port 9011 through the second liquid injection pipeline 911 and is used to provide power for the liquid flowing in the second liquid injection pipeline 911; the second temperature control component 914 is at least used for adjusting the temperature of the liquid flowing through the second liquid injection pipeline 911.
For example, when it is necessary to wash the sample needle 101, a pipetting needle to be subjected to a washing operation is moved into the washing tank 901, a second washing liquid of a preset temperature is supplied to the washing tank 901 through a second liquid injection port 9011 by means of a second liquid injection mechanism 91 to wash the outer wall of the sample needle 101 by means of the second washing liquid, a switching element connected to the control device 70 is provided on a line of the liquid discharge port 9012 communicating with the liquid discharge container to control on or off of the line, wherein the switching element includes, but is not limited to, an electromagnetic valve, a throttle valve, and a waste liquid generated during washing can flow into the waste liquid container through the line by opening the switching element.
It will be appreciated that the second cleaning solution and the first cleaning solution may be the same type of cleaning solution, may be different types of cleaning solutions, and may depend on the detection item performed by the sample analyzer, and are not limited herein.
In some embodiments, the second power component 913 is connected to the second liquid storage container 912 and the second temperature control component 914 through a pipeline, and the second power component 913 includes a switch unit disposed on the pipeline for controlling on-off of the pipeline and a power unit for driving the cleaning liquid to flow in a preset direction in the pipeline. The control device 70 is communicatively connected to a switch unit including but not limited to a solenoid valve and/or a throttle valve and a power unit including but not limited to a pump, a syringe to control the power unit to supply cleaning fluid to the cleaning tank 901 through the second fluid injection port 9011 when cleaning the target device.
In some embodiments, the second liquid injection mechanism 91 and the first liquid injection mechanism 81 are the same liquid injection mechanism, that is, the first cleaning liquid and the second cleaning liquid are the same cleaning liquid, and the separation cleaning device 80 and the cleaning device 90 share a set of liquid injection mechanisms to perform the liquid injection cleaning operation.
The controller 70 is communicatively connected to the dispensing device 10, the sample supply device 20, the reagent supply device 30, the reaction device 40, the mixing device 50, the separation washing device 80, the washing device 80, and the detection device 60, so that at least one of the dispensing device 10, the sample supply device 20, the reagent supply device 30, the reaction device 40, the mixing device 50, the separation washing device 80, the washing device 80, and the detection device 60 performs a predetermined operation, such as a magnetic separation operation of a sample or a detection operation of a sample.
In some embodiments, the controller 70 includes at least a processor 701, a memory 702, a communication interface (not shown), and an I/O interface (not shown). The processor 701, memory 702, communication interfaces, and I/O interfaces communicate over a bus. The processor 701 may be a central processing unit (Central Processing Unit, CPU) which may also be other general purpose processors, digital signal processors (Digital Signal Processor, DSP), application specific integrated circuits (Application Specific Integrated Circuit, ASIC), field-programmable gate arrays (Field-Programmable Gate Array, FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or the like. Wherein the general purpose processor may be a microprocessor or the processor may be any conventional processor or the like.
Various computer programs to be executed by the processor 701, such as an operating system and application programs, are stored in the memory 702, and data necessary for executing the computer programs. During analysis of the sample under test, data stored locally, if needed, may be stored in memory 702. The I/O interface includes, but is not limited to, serial interfaces such as USB, IEEE1394, or RS-232C, parallel interfaces such as SCSI, IDE, or IEEE1284, and analog signal interfaces composed of D/a converters, and the like. An input assembly is coupled to the I/O interface and a user may directly input data to the controller 70 using the input assembly, including but not limited to a keyboard, mouse, touch screen, or control buttons. The display assembly may be communicatively coupled to the controller 70 via an I/O interface for relevant information prompting. The communication interface may be an interface of any communication protocol known at present, the communication interface communicates with the outside through a network, and the controller 70 may transmit data with any component connected through the network through the communication interface in a preset communication protocol.
In some embodiments, the controller 70 is configured to at least: when the liquid injection mechanism is in a non-working state, the power assembly is controlled to suck back air through the liquid injection port, so that liquid in the liquid injection pipeline at least partially flows back to the temperature control assembly, and the non-working state is a state that the liquid injection mechanism does not execute liquid injection operation.
Illustratively, a sample analyzer is taken as an immunoassay analyzer for example, which is a type of high-sensitivity and high-specificity analyzer commonly used in clinical laboratories for detecting various analysis indexes of blood, urine or other body fluids. Conventional immunoassays have various implementation principles such as chemiluminescence, electrochemiluminescence, etc.
Referring to fig. 7, taking a chemiluminescent immunoassay analyzer as an example, the main working principle is as follows: when it is desired to measure a component in a sample, the corresponding antibody/antigen may be coated on a magnetic bead to form a magnetic bead reagent, and a specific label may be labeled on the antibody to form a labeled reagent (the reagent for measuring an analyte item generally has various components, such as a magnetic bead reagent component, a labeled reagent component, etc., herein, and different components of the same item may be packaged in different reagent containers or different chambers of the same reagent container). Firstly, mixing a sample containing an object to be tested with a magnetic bead reagent, a labeling reagent and other reagents to form a sample reagent reaction solution (simply referred to as reaction solution), and incubating under certain conditions to react to form a mixture, wherein the mixture comprises the object to be tested and impurities, and then removing the impurities (such as unbound labeling substance, other reagents and samples) in the mixture by a washing separation (generally abbreviated as B/F) technology; then adding a signal reagent (also called a substrate) into the sample, enabling the marker on the sample to react with the signal reagent (or catalyze the signal reagent) to emit light, detecting the luminous intensity of the sample, and calculating the concentration of the sample component in the sample by using a calibration curve. Wherein the signal reagent can be one or more of luminescent substrate liquid, pre-excitation liquid, luminescence enhancing liquid, etc.
In the process of detecting a sample to be detected by the sample analyzer, the liquid injection mechanism is used for performing liquid injection operation on the liquid container so as to clean the object to be cleaned or the target device in the liquid container, wherein the liquid injection mechanism comprises at least one of a first liquid injection mechanism 81 for separating the cleaning device 80 and a second liquid injection mechanism 91 for separating the cleaning device 90.
Taking the liquid injection mechanism as the first liquid injection mechanism 81 as an example, the first liquid injection mechanism 81 of the separation and cleaning device 80 is used for cleaning the surface of the magnetic particles enriched with the to-be-detected substances in the reaction liquid in the process of performing magnetic separation on the reaction cup 4011 carrying the reaction liquid by the separation and cleaning device 80.
In the magnetic separation process of the separation cleaning device 80, the magnetic particles are magnetically adsorbed and fixed on the wall of the reaction cup 4011 by the adsorption component 802, and at this time, the first cleaning solution is filled into the reaction cup 4011 through the first liquid filling mechanism 81 so as to clean impurities attached to the surface of the magnetic particles, thereby improving the accuracy of the sample detection result.
Based on the fact that the to-be-detected object in the reaction solution is sensitive to the change of the environmental temperature, in order to ensure the accuracy of the sample detection result, the temperature of the first washing liquid injected into the reaction cup 4011 by the first liquid injection mechanism 81 must be controlled within a preset temperature range during the cleaning operation, and if the temperature fluctuation of the first washing liquid is large during the cleaning operation performed by the separation and cleaning device 80, the accuracy of the final sample detection result may be affected.
In the non-working state of the first liquid injection mechanism 81, that is, in a state in which the first liquid injection mechanism 81 does not perform the liquid injection operation, the cleaning liquid stored in the first liquid injection pipeline 811 of the first liquid injection mechanism 81 is affected by the environment, and there may be a certain temperature difference between the temperature of the cleaning liquid and the preset temperature, and if the cleaning liquid having the temperature difference with the preset temperature is used to clean the magnetic particles in the execution cup 4011, the accuracy of the final sample detection result may be affected.
Therefore, in the non-working state of the first liquid injection mechanism 81, the first power component 813 of the first liquid injection mechanism 81 is controlled to suck back air through the first liquid injection port 8011, so that the first cleaning liquid in the first liquid injection pipeline 811 at least partially flows back to the first temperature control component 814, and the first cleaning liquid flowing back is reheated to the preset temperature under the preheating action of the first temperature control component 814, so that the problem that the temperature fluctuation of the cleaning liquid output by the first liquid injection mechanism 81 is large when the liquid injection operation is performed is reduced, and the temperature of the first cleaning liquid injected into the reaction cup 4011 by the first liquid injection mechanism 81 in the working state meets the preset temperature requirement, so that the accuracy of the sample detection result is improved.
Optionally, the volume of the air sucked back when the first power assembly 813 sucks the air back through the first injection port 8011 is a first volume V1, the volume of the cavity between the first injection port 8011 and the first temperature control assembly 814 for accommodating the liquid is a second volume V2, and the first volume V1 is greater than or equal to the second volume V2.
For example, when the first power assembly 813 sucks air back through the first injection port 8011, the volume of the sucked air is greater than or equal to the volume of the cavity between the first injection port 8011 and the first temperature control assembly 814 for accommodating the liquid, so that the liquid between the first temperature control assembly 814 and the first injection port 8011 is sucked back to the first temperature control assembly 814 as much as possible for re-preheating, and the temperature of the first cleaning solution injected into the reaction cup 4011 by the first injection mechanism 81 in the working state is ensured to meet the preset temperature requirement.
It will be appreciated that when the liquid injection mechanism is the second liquid injection mechanism 91 of the cleaning apparatus 90, the second liquid injection mechanism 91 is used to inject liquid into a liquid container to clean a target device in the liquid container, where the liquid container is a cleaning tank 901, and the target device includes, but is not limited to, a sample needle and a reagent needle, and the target device is taken as a sample needle 101, where the sample needle 101 is used to inject a sample into a reaction cup 4011 located at a sample position, so that the sample and the reagent are mixed in the reaction cup 4011 to form a reaction liquid, and in order to improve accuracy of sample analysis, a temperature of the sample needle 101 may affect a temperature of the sample injected through the sample needle 101, and thus, during execution of sample injection into the reaction cup 101, the temperature of the sample needle 101 needs to be within a predetermined temperature range.
Based on the fact that the samples analyzed by the sample analyzer may be different samples, in order to reduce cross infection of the samples during the sample analysis, the sample needle 101 needs to be cleaned during the two sample filling processes performed by the sample needle 101, and in order to make the temperature of the sample needle 101 be within a preset temperature range, the cleaning solution for cleaning the sample needle 101 also needs to be within a preset temperature range, so the temperature of the second cleaning solution injected into the cleaning tank 901 by the second liquid injection mechanism 91 of the cleaning device 90 needs to be kept within a preset temperature range.
Therefore, in the non-working state of the second liquid injection mechanism 91, the second power component 913 of the second liquid injection mechanism 91 is controlled to suck back air through the second liquid injection port 9011, so that the second cleaning liquid in the second liquid injection pipeline 911 is at least partially returned to the second temperature control component 914, and the returned second cleaning liquid is reheated to the preset temperature under the preheating action of the second temperature control component 914, so that the problem that the temperature of the cleaning liquid output by the second liquid injection mechanism 91 fluctuates greatly when the liquid injection operation is performed is reduced, and the temperature of the second cleaning liquid injected into the cleaning tank 901 by the second liquid injection mechanism 81 in the working state meets the preset temperature requirement.
Optionally, the first volume V1 of the air that is drawn back when the second power assembly 93 draws back air through the second injection port 9011 is greater than or equal to the second volume V2 of the cavity between the second injection port 9011 and the second temperature control assembly 814 for containing the liquid.
In some embodiments, the controller 70 is further configured to: controlling the power assembly to suck back air through the liquid injection port when the liquid injection mechanism is in a non-working state and before the liquid injection mechanism executes the liquid injection operation; or controlling the power assembly to suck back air through the liquid injection port after the liquid injection mechanism is in a non-working state and the liquid injection operation is completed.
For example, taking the first liquid injection mechanism 81 as an example, in a non-working state before the first liquid injection mechanism 81 performs the liquid injection operation, or in a non-working state after the first liquid injection mechanism 81 completes the liquid injection operation, the power assembly 813 is controlled to act, so that the first liquid injection mechanism 81 sucks air back through the first liquid injection port 8011, and thus, the liquid between the first temperature control assembly 814 and the first liquid injection port 8011 is sucked back to the first temperature control assembly 814 as much as possible to be preheated again, so as to ensure that the temperature of the first cleaning liquid injected into the reaction cup 4011 by the first liquid injection mechanism 81 meets the preset temperature requirement.
In some embodiments, the controller 70 is further configured to perform: after the liquid injection mechanism finishes the liquid injection operation, determining a time interval between a first time point when the liquid injection mechanism finishes the current liquid injection operation and a second time point when the next liquid injection operation is performed;
when the time interval is longer than the preset time, controlling the power assembly to suck back air through the liquid injection port so as to enable liquid in the liquid injection pipeline to at least partially flow back to the temperature control assembly;
and executing the next liquid injection operation after the liquid in the liquid injection pipeline at least partially flows back to the temperature control assembly.
For example, taking the first liquid injection mechanism 81 as an example, when the time interval between two liquid injection operations performed by the first liquid injection mechanism 81 is greater than the preset time interval, the first cleaning liquid in the first liquid injection pipeline 811 receives an environmental influence, and the temperature difference between the temperature of the first cleaning liquid and the preset temperature may exceed the preset value, then the first power component 813 is controlled to suck back air through the first liquid injection port 8011 between the two liquid injection operations of the liquid injection component of the first liquid injection mechanism 81, so that the first cleaning liquid in the first liquid injection pipeline 811 at least partially flows back to the first temperature control component 814, and the first cleaning liquid flowing back is preheated again by the first temperature control component 814, so that the temperature of the first cleaning liquid output by the first liquid injection mechanism 81 when the next liquid injection operation is performed is within the preset temperature range.
For example, after the first liquid injection mechanism 81 completes the first liquid injection operation performed on the reaction cup 4011 located in the magnetic separation position for the kth time and before the first liquid injection operation performed on the reaction cup 4011 located in the magnetic separation position for the kth time, a first time corresponding to the first liquid injection mechanism 81 performing the first liquid injection operation on the reaction cup 4011 located in the magnetic separation position for the kth time and a second time corresponding to the first liquid injection mechanism 81 performing the first liquid injection operation on the reaction cup 4011 located in the magnetic separation position for the kth time are obtained, and if a time interval between the first time and the second time is greater than a preset time interval, the first power assembly 813 is controlled to suck back air through the first liquid injection port 8011 before the first liquid injection mechanism 81 performs the first liquid injection operation on the reaction cup 4011 located in the magnetic separation position for the kth time, so that the first liquid in the first liquid injection pipeline 811 is at least partially refluxed to the first temperature control assembly 814.
In some embodiments, during heating of the liquid returning in the priming line, the controller 70 is further configured to: and acquiring temperature information of liquid in the liquid injection pipeline, and adjusting heating power of the temperature control component according to the temperature information.
Illustratively, taking the liquid injection mechanism as the first liquid injection mechanism 81 as an example, the heating power of the first temperature control component 814 can be set according to the temperature of the first cleaning liquid in the first liquid injection pipeline 811, so that efficient preheating of the sucked-back first cleaning liquid can be achieved. The temperature information may be obtained by a temperature sensor that may be provided to the first liquid injection line 811, for example, an outer wall of the first liquid injection line 811.
In some embodiments, the sample analyzer 100 further comprises a substrate injection device, a scheduling device, a mixing device 50, and a photometry mechanism, the controller 70 further configured to:
after the pipetting assembly 803 of the separation washing device 80 performs pipetting operation on the cuvette 4011 positioned at the pipetting level of the sample analyzer, controlling the substrate injection device to inject a substrate into the cuvette; after the substrate injection device finishes the substrate injection operation, controlling the dispatching device to dispatch the reaction cup bearing the substrate to the mixing position of the mixing device 50, and controlling the mixing device 50 to perform the mixing operation on the reaction cup 4011 placed at the mixing position so as to fully mix the substrate with the to-be-detected objects in the reaction cup 4011; after the mixing device 50 completes the mixing operation, the reaction cup 401 after the mixing by the scheduling device is controlled to be scheduled to the photometry position of the photometry mechanism of the detecting device 60, and the photometry mechanism is controlled to perform luminescence detection on the reaction cup 4011 placed at the photometry position.
In some embodiments, the controller 70 is further configured to: the control driving assembly drives the pipetting needle to move to the washing tank 901, and controls the second liquid injection mechanism 91 to inject the second washing liquid into the washing tank 901 through the second liquid injection port 9011 so as to wash the pipetting needle located in the washing tank 901.
Illustratively, taking a pipetting needle as the sample needle 101 as an example, during a cleaning operation of the sample needle 101 by the second liquid injection mechanism 91, the controller 70 controls the first driving assembly 102 to drive the sample needle 101 to move to the cleaning tank 901 and controls the second liquid injection mechanism 91 to inject the second cleaning liquid into the cleaning tank 901 through the second liquid injection port 9011 so as to clean the outer wall of the sample needle located in the cleaning tank 901.
A method for controlling injection according to the embodiment of the present application will be described below with reference to the working principle of the sample analyzer 100.
The liquid injection control method comprises the following steps: and controlling the power assembly of the liquid injection mechanism to suck back air through the liquid injection port of the liquid injection mechanism when the liquid injection mechanism of the sample analyzer is in a non-working state, so that liquid in the liquid injection pipeline of the liquid injection mechanism at least partially flows back to the temperature control assembly of the liquid injection mechanism, wherein the non-working state is a state that the liquid injection mechanism does not execute liquid injection operation.
In some embodiments, when the liquid injection mechanism is in a non-working state, controlling the power assembly to suck back air through the liquid injection port comprises:
controlling the power assembly to suck back air through the liquid injection port when the liquid injection mechanism is in a non-working state and before the liquid injection mechanism executes the liquid injection operation;
Or controlling the power assembly to suck back air through the liquid injection port after the liquid injection mechanism is in a non-working state and the liquid injection operation is completed.
In some embodiments, the method further comprises: after the liquid injection mechanism completes the liquid injection operation, determining a time interval between a first time point when the liquid injection mechanism completes the current liquid injection operation and a second time point when the next liquid injection operation is executed; when the time interval is longer than the preset time, controlling the power assembly to suck back air through the liquid injection port, so that liquid in the liquid injection pipeline at least partially flows back to the temperature control assembly, and executing the next liquid injection operation after the liquid in the liquid injection pipeline at least partially flows back to the temperature control assembly.
In some embodiments, during heating of the liquid returning in the liquid injection line, the method further comprises: and acquiring temperature information of the liquid in the liquid injection pipeline, and adjusting heating power of the temperature control component according to the temperature information.
In some embodiments, the sample analyzer further comprises a substrate injection device, a scheduling device, a mixing device, and a photometry mechanism, the method further comprising:
After the liquid sucking operation of the liquid sucking assembly on the reaction cup positioned at the liquid sucking position is finished, controlling the substrate injecting device to inject a substrate into the reaction cup;
after the substrate injection device finishes the substrate injection operation, controlling the dispatching device to dispatch the reaction cup bearing the substrate to a mixing position of the mixing device, and controlling the mixing device to perform the mixing operation on the reaction cup placed at the mixing position;
after the mixing device completes the mixing operation, controlling the reaction cup after the mixing device is uniformly mixed to be scheduled to the photometry position of the photometry mechanism, and controlling the photometry mechanism to carry out luminescence detection on the reaction cup placed at the photometry position.
In some embodiments, the liquid stored in the liquid storage container is a second cleaning liquid, and the sample analyzer further comprises: a dispensing device comprising a pipetting needle for transferring samples and/or reagents and a drive assembly for driving the pipetting needle to move; the liquid injection port of the liquid injection mechanism is positioned on the inner wall of the cleaning tank so as to inject liquid into the cleaning tank through the liquid injection port; the method further comprises the steps of:
And controlling the driving assembly to drive the pipetting needle to move to the cleaning pool, and controlling the liquid injection mechanism to inject a second cleaning liquid into the cleaning pool through the liquid injection port so as to clean the pipetting needle positioned in the cleaning pool.
It should be noted that, for convenience and brevity of description, specific working procedures of the above-described liquid injection control method may refer to corresponding working procedures of the above-described sample analyzer, and will not be described herein.
It is to be understood that the terminology used in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this specification and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.
It should also be understood that the term "and/or" as used in this specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations. It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or system that comprises the element.
The foregoing embodiment numbers of the present application are merely for describing, and do not represent advantages or disadvantages of the embodiments. While the invention has been described with reference to certain preferred embodiments, it will be understood by those skilled in the art that various changes and substitutions of equivalents may be made and equivalents will be apparent to those skilled in the art without departing from the scope of the invention. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (10)
1. A sample analyzer, comprising:
a liquid container;
the liquid injection mechanism is provided with a liquid injection port and is used for executing liquid injection to the liquid container through the liquid injection port, and comprises a liquid injection pipeline, a liquid storage container, a power component and a temperature control component, wherein the liquid storage container is used for storing liquid, the temperature control component is communicated between the liquid injection port and the liquid storage container, and the power component is communicated with the liquid injection port through the liquid injection pipeline and is used for providing power for the flowing of the liquid in the liquid injection pipeline; the temperature control component is at least used for adjusting the temperature of the liquid flowing through the liquid injection pipeline; and
The controller is connected with the liquid injection mechanism and is used for:
and controlling the power assembly to suck back air through the liquid injection port when the liquid injection mechanism is in a non-working state, so that liquid in the liquid injection pipeline at least partially flows back to the temperature control assembly, wherein the non-working state is a state that the liquid injection mechanism does not execute liquid injection operation.
2. The sample analyzer of claim 1, wherein the controller is further configured to:
controlling the power assembly to suck back air through the liquid injection port when the liquid injection mechanism is in a non-working state and before the liquid injection mechanism executes the liquid injection operation;
or controlling the power assembly to suck back air through the liquid injection port after the liquid injection mechanism is in a non-working state and the liquid injection operation is completed.
3. The sample analyzer of claim 1, wherein the controller is further configured to:
after the liquid injection mechanism completes the liquid injection operation, determining a time interval between a first time point when the liquid injection mechanism completes the current liquid injection operation and a second time point when the next liquid injection operation is executed;
When the time interval is longer than the preset time, controlling the power assembly to suck back air through the liquid injection port so as to enable liquid in the liquid injection pipeline to at least partially flow back to the temperature control assembly;
and executing the next liquid injection operation after the liquid in the liquid injection pipeline at least partially flows back to the temperature control assembly.
4. The sample analyzer of claim 1, wherein the volume of air that is drawn back through the port is a first volume; the volume of the cavity between the liquid injection port and the temperature control component for containing liquid is a second volume, and the first volume is larger than or equal to the second volume.
5. The sample analyzer of claim 1, wherein the controller is further configured to:
and acquiring temperature information of the liquid in the liquid injection pipeline, and adjusting heating power of the temperature control component according to the temperature information.
6. The sample analyzer of claim 1, wherein the liquid container is a reaction cup, the reaction cup carries a reaction liquid, and the liquid stored in the liquid storage container is a first cleaning liquid; the sample analyzer also comprises a magnetic separation disc, an adsorption assembly and a liquid suction assembly, wherein the magnetic separation disc is provided with at least one magnetic separation position, is of a rotatable structure, and is provided with at least one liquid injection level and at least one liquid suction level along the rotation path;
The adsorption component is provided with at least one magnetic adsorption component capable of adsorbing magnetic particles in the reaction liquid in the reaction cup at the magnetic separation position;
the liquid injection mechanism is used for injecting a first cleaning liquid into the reaction cup which moves to the liquid injection level so as to clean impurities attached to the surfaces of the magnetic particles;
the liquid sucking component is used for executing liquid sucking operation to the reaction cup which moves to the liquid sucking position.
7. The sample analyzer of claim 6, further comprising a substrate injection device, a scheduling device, a blending device, and a photometry mechanism, the controller further configured to:
after the liquid sucking operation of the liquid sucking assembly on the reaction cup positioned at the liquid sucking position is finished, controlling the substrate injecting device to inject a substrate into the reaction cup;
after the substrate injection device finishes the substrate injection operation, controlling the dispatching device to dispatch the reaction cup bearing the substrate to a mixing position of the mixing device, and controlling the mixing device to perform the mixing operation on the reaction cup placed at the mixing position;
after the mixing device completes the mixing operation, controlling the reaction cup after the mixing device is uniformly mixed to be scheduled to the photometry position of the photometry mechanism, and controlling the photometry mechanism to carry out luminescence detection on the reaction cup placed at the photometry position.
8. The sample analyzer of any one of claims 1-7, wherein the priming mechanism further comprises a priming needle in communication with the priming line, the priming port being disposed at an end of the priming needle.
9. The sample analyzer of claims 1-5, wherein the fluid stored in the fluid reservoir is a second cleaning fluid, the sample analyzer further comprising:
a dispensing device comprising a pipetting needle for transferring samples and/or reagents and a drive assembly for driving the pipetting needle to move;
the liquid container is a cleaning tank, and a liquid injection port of the liquid injection mechanism is positioned on the inner wall of the cleaning tank so as to inject liquid into the cleaning tank through the liquid injection port;
the controller is further configured to:
and controlling the driving assembly to drive the pipetting needle to move to the cleaning pool, and controlling the liquid injection mechanism to inject a second cleaning liquid into the cleaning pool through the liquid injection port so as to clean the pipetting needle positioned in the cleaning pool.
10. A method of controlling priming for a sample analyzer, the method comprising:
and controlling the power assembly of the liquid injection mechanism to suck back air through the liquid injection port of the liquid injection mechanism when the liquid injection mechanism of the sample analyzer is in a non-working state, so that liquid in the liquid injection pipeline of the liquid injection mechanism at least partially flows back to the temperature control assembly of the liquid injection mechanism, wherein the non-working state is a state that the liquid injection mechanism does not execute liquid injection operation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202211174619.5A CN117805357A (en) | 2022-09-26 | 2022-09-26 | Sample analyzer and liquid injection control method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211174619.5A CN117805357A (en) | 2022-09-26 | 2022-09-26 | Sample analyzer and liquid injection control method |
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| CN117805357A true CN117805357A (en) | 2024-04-02 |
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| CN202211174619.5A Pending CN117805357A (en) | 2022-09-26 | 2022-09-26 | Sample analyzer and liquid injection control method |
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- 2022-09-26 CN CN202211174619.5A patent/CN117805357A/en active Pending
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