CN110146715A - A high-throughput automatic immunoluminescence analysis system - Google Patents

Abstract

The invention discloses a high-throughput automatic luminescence immunoassay system, which includes a sample loading bracket, a sample loading arm, a sample loading needle, a reagent chamber, a sample chamber, a reaction incubation plate, a reaction turntable, a reaction cup groove, a reaction cup, and a reaction cup automatic Cup loading assembly, reaction cup clamping arm, cleaning station assembly, insulation tray, insulation turntable, substrate needle, measurement darkroom assembly and photon measurement assembly; use multiple sample loading arms to add samples or reagents at the same time, improving the addition time per unit time The number of cuvettes for samples or reagents can be reduced to increase the throughput of the system; as required, substrate needles can be set up outside the measurement darkroom assembly or outside to meet the measurement requirements of the flash method or glow method. In some cases, the cuvette after adding the substrate can be delayed and kept warm as needed, the photon measurement component can measure the product in the cuvette, and the computer can calculate the concentration of the analyte according to the measurement result.

Description

A high-throughput automatic immunoluminescence analysis system

technical field

The invention relates to the technical field of biological monitoring and clinical testing equipment, in particular to a high-throughput fully automatic immunoluminescent analysis system.

Background technique

With the development of science and technology, chemiluminescence and bioluminescence measurements have become very common in recent years. They are often used to determine the content of an unknown component in a sample, and in the past decade, luminescence measurements have been used in It also plays an important role in the study of gene expression and regulation. Chemiluminescence is the use of light excited during chemical reactions, and biochemical luminescence is the use of light emitted by enzymatic reactions during chemical reactions. Compared with other measurement techniques, chemical and biochemical luminescence measurement techniques have the following advantages: extremely high sensitivity, wide dynamic range, and emerging luminescence measurement reagents. Luminescence measurement has extremely high sensitivity, which is 10 ⁵ times more sensitive than spectral absorption measurement technique, and at least 1000 times higher than fluorescence measurement technique.

However, the existing automatic immunoluminescence analyzer uses the XYZ movement mechanism to increase the stroke of the sample loading arm, resulting in a relatively low number of cuvettes with samples or reagents added per unit time, and a relatively slow sample processing speed.

Contents of the invention

The purpose of the present invention is to provide a high-throughput fully automatic immunoluminescence analysis system, which further improves the problems raised by the background technology, uses multiple sample loading arms to add samples or reagents at the same time, and improves the efficiency of adding samples per unit time. Or the number of reaction cups for reagents, the number of reaction cups that have added samples and reagents per hour can reach up to 720 times, thereby improving the throughput of the system; at the same time, the bottom can be set in the measurement darkroom component or its external exterior as needed In order to meet the measurement requirements of the flash method or glow method; in the case of adding the substrate externally, the system after adding the substrate can be delayed and kept warm according to the needs, and the photon measurement component can measure the product in the cuvette. Measurement is carried out, and the computer calculates the concentration of the analyte according to the measurement result.

The purpose of the present invention can be achieved through the following technical solutions:

A high-throughput automatic luminescent immunoassay system, including a sample loading rack, a sample loading arm, a sample loading needle, a reagent chamber, a sample chamber, a reaction incubation plate, a reaction turntable, a reaction cup groove, a reaction cup, and an automatic cup filling assembly for the reaction cup , reaction cup clamping arm, cleaning station assembly, insulation tray, insulation turntable, substrate needle measurement, measurement darkroom assembly and photon measurement assembly, the reaction incubator is equipped with an axially rotating reaction turntable, and the reaction cup automatic filling assembly is located in the reaction chamber. Above the turntable, the photon measurement assembly is fixedly installed on one side of the measurement darkroom assembly, and it is characterized in that the sample loading bracket is distributed on the side of the reaction turntable, the sample loading bracket is equipped with a sample loading arm, and the sample loading arm is fixedly installed with Sample needle.

Reaction cup clamping arms are installed between the reaction turntable and the cleaning station assembly and between the cleaning station assembly and the measurement darkroom assembly.

The heat preservation plate is located inside the cleaning station assembly, and an axially rotating heat preservation turntable is installed on the heat preservation plate, and the substrate needle is fixedly installed on the heat preservation plate through the substrate needle support.

Further, the reagent chamber and the sample chamber are respectively located on both sides of the reaction turntable.

Further, the reaction turntable is provided with two rows of reaction cup grooves distributed axially.

Further, the cuvette clamping arm moves along the radial direction of the reaction turntable, the direction of the Z axis and the rotation.

Further, the sample loading arm moves along the X-axis direction, the Z-axis direction and rotates.

Further, the sample loading arms are divided into two groups, the sample loading arms located on one side of the reagent chamber are reagent arms, and the sample loading arms located on one side of the sample chamber are sample arms.

Further, there is a time interval between the movement of the sample arms.

There is a time interval between the movements of the reagent arms.

Further, the cuvette automatic cup filling assembly automatically fills the cuvette slots in the inner and outer rings of the reaction turntable.

Further, the test tube rack transport system transports the test tube rack to the lower part of the corresponding sample arm, and the reagent box transport system transports the reagent box to the lower part of the corresponding reagent arm.

Further, the reagent box conveying system arranges and places the reagent boxes in the reagent compartment.

Beneficial effects of the present invention:

1. The present invention uses multiple sample loading arms to add samples or reagents at the same time, increasing the number of cuvettes that have added samples or reagents per unit time, thereby increasing the throughput of the system;

2. The present invention can set up a substrate adding component outside the measurement darkroom component or its exterior as required, so as to meet the measurement requirements of the flash method or the glow method;

3. In the case of adding the substrate externally, the present invention can delay and insulate the system after adding the substrate as required;

4. The reaction turntable of the present invention is equipped with double rows of reaction cup slots distributed in the axial direction, and the reaction cup automatic cup filling assembly can automatically fill the reaction cup slots in the inner and outer rings of the reaction turntable, increasing the load of the reaction turntable for loading reaction cups;

5. The present invention uses the test tube rack delivery system to transport the test tube rack to the bottom of the corresponding sample arm, and the reagent box delivery system transports the reagent box to the bottom of the corresponding reagent arm, and moves the kit/test tube rack to the sample loading arm compared to the traditional manual Below, the manual labor intensity is reduced, and the working time is reduced. At the same time, the reagent boxes in the reagent compartment are arranged in the reagent compartment through the conveying mechanism, further reducing the manual labor intensity.

Description of drawings

The present invention will be further described below in conjunction with the accompanying drawings.

Fig. 1 is a schematic structural diagram of the high-throughput fully automatic immunoluminescence analysis system of the present invention;

Fig. 2 is a partial structure schematic diagram of the present invention;

Fig. 3 is a structural schematic diagram of different viewing angles of the present invention;

Fig. 4 is a structural schematic diagram of the sample loading arm of the present invention;

Fig. 5 is a schematic structural diagram of the kit delivery system of the present invention;

Fig. 6 is a schematic diagram of the structure of the robotic arm for rotating the kit of the present invention;

Fig. 7 is a schematic diagram of the structure of the kit handling robot arm of the present invention;

Fig. 8 is a schematic structural view of the test tube rack conveying system of the present invention;

Fig. 9 is a structural schematic diagram of different viewing angles of the test tube rack delivery system of the present invention;

Fig. 10 is a schematic diagram of the structure of the delivery channel of the present invention;

Fig. 11 is a three-dimensional sectional view of the delivery channel of the present invention;

Fig. 12 is a schematic structural view of the cuvette clamping arm of the present invention;

Fig. 13 is a schematic diagram of a partial structure of a cuvette clamping arm of the present invention;

Fig. 14 is a schematic diagram of a partial structure of the present invention;

Fig. 15 is a schematic diagram of an enlarged structure at point A of the present invention.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

In describing the present invention, it is to be understood that the terms "opening", "upper", "lower", "thickness", "top", "middle", "length", "inner", "surrounding" etc. Indicating orientation or positional relationship is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as limiting the present invention .

As shown in Figure 1, Figure 2, Figure 14 and Figure 15, a high-throughput automatic immunoluminescence analysis system includes a system bracket 1, a system base plate 01, a sample loading bracket 2, a sample loading arm 3, and a sample loading needle 4 , Reagent chamber 5, Sample chamber 6, Reaction incubation plate 7, Reaction turntable 8, Reaction cup tank 9, Reaction cup 10, Reaction cup automatic filling assembly 11, Reaction cup clamping arm 12, Cleaning station assembly 13, Insulation tray 14, Incubation turntable 15, substrate needle 16, measurement darkroom assembly 17 and photon measurement assembly 18.

The system backplane 01 is fixedly installed on the system bracket 1 .

The sample loading rack 2 , the reaction incubation tray 7 , the cleaning station assembly 13 and the measurement darkroom assembly 17 are all fixedly installed on the system bottom plate 01 .

The reagent chamber 5 and the sample chamber 6 are respectively located on both sides of the reaction turntable 8, and the reagent chamber 5 and the sample chamber 6 are fixedly installed on the system bracket 1, the reagent chamber 5 is placed with arranged reagent boxes, and the sample chamber 6 is placed with arranged and distributed reagent boxes. test tube rack.

The reaction cup automatic cup loading assembly 11 is located above the reaction turntable 8 and is fixedly installed on the system support 1 .

Reaction cup clamping arms 12 are fixedly installed on the system base plate 01 between the reaction turntable 8 and the cleaning station assembly 13 and between the cleaning station assembly 13 and the measurement chamber assembly 17 .

As shown in Figure 3, an axially rotating reaction turntable 8 is installed on the reaction incubator 7, and the reaction turntable 8 is provided with a double row of axially distributed reaction cup grooves 9, so that the reaction turntable 8 is relatively different from the original single row of reaction cup grooves 9. More reaction cups 10 can be loaded at one time, and part of the reaction cup groove 9 is in the reaction incubating plate 7, and the reaction cup 10 is placed in the reaction cup groove 9, so that the reaction cup 10 placed in the reaction cup groove 9 is kept at a constant temperature. For incubation, the upper end of the reaction incubation plate 7 is covered with a heat preservation cover.

The photon measurement component 18 is fixedly installed in one side of the measurement darkroom component 17 .

The sample loading bracket 2 is distributed on the 8 peripheral sides of the reaction turntable, and the sample loading bracket 2 is equipped with a sample loading arm 3 (or the upper ends of the sample loading bracket 2 on the same side are connected together to form a sample loading arm guide rail, and two sample loading arm guide rails are installed. A loading arm 3).

As shown in Figure 4, the sample loading arm 3 includes a sample loading X-axis support base 31, a sample loading Z-axis support base 32, a sample loading motor 33 and a sample injection needle support 34, and a sample loading X-axis support base 31 is equipped with a Z-axis supporting base 32, sample loading Z-axis supporting base 32 is fixedly installed with a sample feeding motor 33, and a sample feeding needle support 34 is fixedly installed on the rotating shaft at the output end of the sample feeding motor 33.

The sample loading bracket 2 drives the sample loading X-axis support seat 31 to move along the X-axis direction through the sample loading X-axis timing belt 35, and the sample loading X-axis support seat 31 drives the sample loading Z-axis support seat 32 through the sample loading Z-axis timing belt 36 It moves along the Z-axis direction, wherein the sample loading X-axis timing belt is fixedly installed on the sample loading bracket 2, and the sample loading Z-axis timing belt 36 is fixedly installed on the sample loading X-axis support seat 31.

A sampling needle 4 is fixedly installed on the sampling needle bracket 34, and the sampling needle 4 is connected to the sampling pump through a pipeline.

When in use, the sampling needle 4 moves in the X-axis direction and the Z-axis direction through the X-axis synchronous belt 35 and the sampling Z-axis synchronous belt 36, and the sampling needle 4 rotates around the output end of the sampling motor 33 through the sampling motor 33. .

Divide the sample loading arm 3 into two groups, the sample loading arm 3 on one side of the reagent chamber 5 is the reagent arm, and the sample loading arm 3 on the side of the sample chamber 6 is the sample arm. When measuring, place the transparent cuvette 10 in the reaction chamber. In the turntable 8, the reaction turntable 8 rotates. When the reaction turntable 8 drives the cuvette 10 to rotate to the vicinity of the reagent arm, a single reagent arm passes through the X-axis direction, Z-axis direction, rotational movement and sample addition of the sample needle 4 of the sample support 2. The pump, the sample injection needle 4, and the reagent arm add the reagent in the reagent chamber 5 to the reaction cup 10. After the reagent in the reaction cup 10 is added, the reaction turntable 8 drives the reaction cup 10 to rotate to the vicinity of the sample arm, and a single sample arm passes through the X-axis direction, Z-axis direction, rotational movement of the sample loading arm 3 of the support 2 and the sample loading pump and the sample injection needle 4 , the sample arm adds the sample in the sample chamber 6 to the same cuvette 10 .

The above sequence of adding reagents and samples to the cuvette 10 is not limited to adding the reagents first and then adding the sample, the cuvette 10 can also be driven by the reaction turntable 8 to rotate to the vicinity of the sample arm, the sample is added first, and then the cuvette is driven by the reaction turntable 8 10Turn around the reagent arm and add reagent.

The above-mentioned movement relationship between the sample arms and the movement relationship between the reagent arms are all performed asynchronously, and there is a time interval between them.

Use two sample arms and two reagent arms to add samples and reagents to the reaction cups 10 on the reaction turntable 8, and add samples and reagents to the two reaction cups 10 at the same time, compared with the traditional single sample arm and reagent arm at the same time. Adding samples and reagents to the cuvettes 10 doubles the number of cuvettes 10 that have been added with samples or reagents per unit time, thereby increasing the throughput of the system.

The sampling needle 4 performs sampling through the X-axis direction, the Z-axis direction and the rotational movement, compared with the traditional sampling needle 4 through the X-axis direction, the Y-axis direction and the Z-direction movement to add samples, reducing the number of needles during the sampling process. 4 Sampling and adding the reagent or sample to the stroke of the cuvette 10, and further increasing the adding speed of the reagent or sample in the cuvette 10.

As shown in Figures 1 and 5, the front end of the reagent compartment 5 is provided with a reagent box inlet 51, and the rear end of the reagent compartment 5 is provided with a reagent box outlet 52, and reagent boxes are fixedly installed inside the reagent box inlet 51 and the reagent box outlet 52. Chute 53, transfer chute 54 is installed between the reagent box chute 53, transfer chute 54 is fixedly installed on the reagent warehouse 5, wherein reagent box entrance 51, reagent box chute 53, transfer chute 54 and reagent box outlet 52 is kept in a straight line, and a reagent box delivery system 55 is installed in the reagent compartment 5 .

The reagent box conveying system 55 includes a reagent box pushing plate 551, a reagent box rotating and carrying mechanical arm 552 and a reagent box carrying mechanical arm 553. The reagent box slides along the reagent box chute 53 to the side of the reagent box rotating and conveying robot arm 552, and the reagent box rotating and conveying robot arm 552 holds up the reagent box, and the reagent box rotating and conveying robot arm 552 is lifted by the conveying synchronous belt 555. The reagent box is transported, transported to the target position of the reagent compartment 5, and the reagent boxes are arranged. The reagent box transporting robot arm 553 is located on the side of the reagent box rotating transporting robot arm 552.

When the reagent in the reagent box needs to be sucked, the reagent box rotating and transporting mechanical arm 552 transports the target reagent box in the reagent chamber 5 to the bottom of the sample loading arm 3 through the transport timing belt 555, and the 4 pairs of sample loading needles on the sample loading arm 3 The reagent in the target reagent box is extracted, and the empty reagent box is transported to the initial position of the reagent compartment 5 by the reagent box rotating and transporting robot arm 552 again.

After the reagents in all the reagent boxes in the reagent compartment 5 have been extracted, the reagent box carrying mechanical arm 553 lifts up the empty reagent box on the side of the reagent box outlet 52 of the reagent compartment 5, and the reagent box transporting mechanical arm 553 passes through the transport timing belt. 555 transports the lifted reagent box to the reagent box chute 53 on the side of the reagent box outlet 52; the reagent box rotating and transporting mechanical arm 552 lifts up the reagent box 5 that is empty on the side of the reagent box inlet 51 , the reagent box rotating and transporting mechanical arm 552 transports the lifted reagent box to the reagent box chute 53 on the side of the reagent box inlet 51 through the conveying timing belt 555, and the reagent box pushing plate 551 pushes the empty reagent box by pushing the timing belt 554. The reagent box slides to the reagent box outlet 52 along the reagent box chute 53 or the transfer chute 54, and is pushed out from the reagent box outlet 52 to complete the collection.

As shown in Figure 6, the reagent box rotating and transporting mechanical arm 552 includes a motor mounting base 5521, a lifting support portion 5522, a reagent box clamping piece 5523, a reagent box rotating pulley 5524 and a lifting guide rail 5525, wherein the motor mounting base 5521 is synchronized with the handling The belt 555 is fixedly connected, and the motor mounting base 5521 is equipped with a lifting support part 5522 which is slidingly connected. Pulley 5524, the kit rotation pulley 5524 is equipped with a lifting guide rail 5525 slidingly connected along the Z-axis direction, and the lifting guide rail 5525 is fixedly connected with the lower end of the lifting support part 5522.

The motor mounting base 5521 drives the lifting support part 5522 to move along the Z-axis direction by arranging the Z-axis synchronous belt 5526, and the motor mounting base 5521 drives the kit rotating pulley 5524 to rotate by arranging the rotating synchronous belt 5527, and the kit rotating pulley 5524 passes through the lifting The guide rail 5525 drives the lifting support part 5522 to rotate.

When in use, the reagent box clamping piece 5523 moves in the Z-axis direction to hold up the plug portion of the reagent box, and when it is necessary to use the reagent box rotating and transporting mechanical arm 552 to transport the reagent box to the reagent compartment 5 located on the side of the reagent box outlet 52 During inner arrangement, the lifting support part 5522 rotates, and drives the lifted reagent box to rotate to the reagent compartment 5 located on the side of the reagent box outlet 52 for arrangement.

As shown in Figure 7, the reagent box transporting mechanical arm 553 includes a transporting motor mounting base 5531, a transporting lifting support portion 5532 and a transporting reagent box clamping piece 5533, wherein the transporting motor mounting base 5531 is fixedly connected to the transporting synchronous belt 555, and the transporting motor is installed The transport lifting support part 5532 that is slidingly connected is installed on the seat 5531, and the transport motor mounting base 5531 drives the transport lifting support part 5532 to move along the Z axis direction by moving away from the Z-axis synchronous belt 5534, and the transport lifting support part 5532 is fixedly installed with a transport The reagent box clamping piece 5533, when in use, transports the reagent box clamping piece 5533 to lift and transport the plug portion of the reagent box by moving in the Z-axis direction.

As shown in Figures 8 and 9, a test tube rack conveying system 61 is installed in the sample chamber 6, and the test tube rack conveying system 61 includes a test tube rack Y-axis support seat 611, a test tube rack Z-axis support seat 612, a test tube rack rotating support seat 613, Test tube rack supporting seat 614, test tube rack motor 615, test tube rack supporting portion 616 and test tube rack clamping portion 617, test tube rack Y-axis supporting seat 611 is installed on the sample warehouse 6, and a sliding connection is installed on the test tube rack Y-axis supporting seat 611. The test tube rack Z-axis support seat 612, the test tube rack Z-axis support seat 612 upper end is fixedly equipped with a test tube rack rotating support seat 613, and a test tube rack supporting seat 614 slidingly connected along the Z-axis direction is installed on the test tube rack rotating support seat 613, A test tube rack motor 615 is fixedly installed on the test tube rack support seat 614, and a test tube rack outer gear 6151 is fixedly installed on the driving shaft of the test tube rack motor 615, and the test tube rack outer gear 6151 is meshed with a test tube rack outer rack 6152, and the test tube rack outer gear A test tube rack holding part 616 is fixedly installed on the top of the bar 6152, and a test tube rack holding part 617 is fixedly installed on the test tube rack holding part 616.

Wherein, the Y-axis support seat 611 of the test tube rack drives the Z-axis support seat 612 of the test tube rack to move along the Y-axis support seat 611 of the test tube rack through the Y-axis synchronous belt 618 of the test tube rack, so as to realize the movement of the test tube rack supporting part 616 along the Y-axis direction , the test tube rack Y-axis timing belt 618 is fixedly installed on the test tube rack Y-axis support seat 611 .

The test tube rack Z-axis support seat 612 is fixedly installed with the test tube rack Z-axis synchronous belt 619, and the test tube rack Z-axis synchronous belt 619 is fixedly installed with the test tube rack supporting seat 6191, and the test tube rack supporting seat 6191 is connected with the test tube rack supporting seat. The lower end of 614 is rotationally connected; the test tube rack supporting seat 614 is driven to move along the Z-axis direction by the test tube rack Z-axis synchronous belt 619, further realizing the movement of the test tube rack supporting part 616 along the Z-axis direction.

The test tube rack rotating support seat 613 upper end is equipped with a test tube rack rotating synchronous belt 620, and the test tube rack rotating synchronous belt 620 is internally engaged with a test tube rack rotating seat 621, and the test tube rack rotating supporting seat 613 passes through the test tube rack rotating synchronous belt 620 and the test tube rack rotating seat. 621 drives the test tube rack supporting seat 614 to rotate around itself, so as to realize the rotation of the test tube rack supporting part 616.

During use, the test tube rack supporting part 616 moves in the Y-axis direction and the Z-axis direction to move the test tube rack supporting part 616 to the bottom of the target test tube rack in the sample chamber 6. By turning on the test tube rack motor 615, the test tube rack motor 615 The test tube rack outer gear 6151 on the drive shaft rotates to drive the test tube rack outer rack 6152 to move, and the test tube rack outer rack 6152 drives the test tube rack supporting part 616 to insert into the bottom of the target test tube rack, and supports the bottom of the target test tube rack; Among them, the test tube rack locking part 617 clamps the sample tube on the target test tube rack to prevent loosening between the target test tube rack and the test tube rack conveying system 61 during the transportation process, and the test tube rack holding part 616 moves in the Y-axis direction and Movement in the Z-axis direction transports the target test tube rack to the bottom of the sample arm, wherein the test tube rack holding part 616 realizes the processing of the test tube rack on the other side of the sample chamber 6 through its own rotation, and the samples in the test tube rack are completely absorbed by the sampling needle Afterwards, the test tube rack conveying system 61 transports the test tube rack and returns to the original position in the sample chamber 6 .

Both the reagent chamber 5 and the sample chamber 6 contain a conveying mechanism, which continuously transports the reagent box of the reagent chamber 5 or the test tube rack of the sample chamber 6 to the bottom of the sample loading arm 3. Compared with the traditional manual movement of the reagent box/test tube rack to the sample loading Below the arm 3, the manual labor intensity is reduced, and the time consumption of work is reduced at the same time. At the same time, the reagent boxes in the reagent compartment 5 are automatically arranged in the reagent compartment 5 through the conveying mechanism, further reducing the manual labor intensity.

As shown in Figure 1, the cuvette automatic cup filling assembly 11 includes a cuvette arrangement device 111 (such as a large-capacity fully automatic cuvette arrangement device in patent No. CN201311274346. The shell of the device 111 is fixedly installed on the system bracket 1, and the cuvettes 10 can be continuously loaded into the reaction turntable 8 through the cuvette arrangement device 111, the slideway 112, and the delivery channel 113. The cuvette arrangement device 111 makes the cuvettes in the hopper 10 falls into the slideway 112 according to the determined orientation, and falls into the cuvette groove of the cuvette 10 through the conveying channel 113 .

As shown in Figure 11 and Figure 12, the delivery channel 113 includes a channel body 1131, a limit electromagnet 1132, a drive motor 1133, a stop bar 1134, an outer ring channel 1135, an inner ring channel 1136 and a reflective photoelectric switch 1137, and the channel body 1131 The upper end communicates with the slideway 112, the cuvette 10 in the slideway 112 falls into the channel body 1131, a pair of limit electromagnets 1132 are installed outside the channel body 1131, the limit electromagnet 1132 is activated, and the limit electromagnet 1132 at the lower end The drive shaft at the output end bears against the cuvette 10 in the channel body 1131, and the limit electromagnet 1132 at the upper end squeezes the cuvette 10 in the channel body 1131 to limit the position of the cuvette 10. The outside of the channel body 1131 A driving motor 1133 is installed, and a retaining bar 1134 is fixedly installed on the driving shaft of the driving motor 1133. An outer ring channel 1135 and an inner ring channel 1136 are fixedly installed at the lower end of the channel body 1131, wherein the outlets at the lower ends of the outer ring channel 1135 and the inner ring channel 1136 are respectively located at Directly above the outer and inner rings of the reaction turntable 8 , reflective photoelectric switches 1137 are fixedly mounted on the upper and lower ends of the channel body 1131 .

A cuvette limiting cover 1138 is fixedly installed on the blocking bar 1134 .

When in use, start the limit electromagnet 1132, the drive shaft at the output end of the limit electromagnet 1132 limits the position of the cuvette 10 in the channel body 1131, and the single cuvette 10 in the channel body 1131 at the top of the bar 1134 falls due to its own gravity onto the bar 1134, and slide along the bar 1134 into the outer channel 1135 or the inner channel 1136. During this process, the cuvette limit cover 1138 limits the cuvette 10 that slides on the bar 1134 to prevent During the sliding down process of the cuvette 10, the cuvette 10 turns over by itself.

Start the drive motor 1133, the drive shaft of the drive motor 1133 drives the bar 1134 to swing, and by changing the swing direction and angle of the bar 1134, the selective outer ring channel 1135 and the inner ring channel 1136 are used to drop cups, thereby realizing the reaction of the turntable 8 The cuvette groove 9 of the outer ring or the inner ring performs automatic filling of the cuvette 10 .

During this process, the reflective photoelectric switch 1137 located at the lower end of the channel body 1131 monitors the cuvette 10 at the top of the bar 1134. When there is a cuvette 10 at the top of the bar 1134, the bar 1134 swings normally; When there is no cuvette 10 at the top, there are two cases at this time. One is that the cuvette 10 at the upper end of the stop bar 1134 falls to the outer ring channel 1135 or the inner ring channel 1136. At this time, the drive shaft at the output end of the limit electromagnet 1132 shrinks to The cuvette 10 in the channel body 1131 will be dropped next time, and the other is that the cuvette 10 is placed upside down on the bar 1134, and the driving motor 1133 drives the upper end of the bar 1134 to rotate to the outer channel 1135 or the inner channel 1136 Nearby, during this process, the cuvette 10 covered by the top end of the bar 1134 slides down into the outer ring channel 1135 or the inner ring channel 1136 due to its own gravity.

The reflective photoelectric switch 1137 located at the upper end of the channel body 1131 monitors the cuvette 10 in the upper end of the channel body 1131. When the reflective photoelectric switch 1137 monitors that there is a cuvette 10 at the upper end of the channel body 1131, it indicates that the cuvette 10 in the channel body 1131 is present. The cuvettes 10 have been backlogged and full, so the cuvette arrangement device 111 is controlled to stop running to prevent cuvettes 10 from accumulating in a large amount in the channel body 1131 and the slideway 112; when the reflective photoelectric switch 1137 at the upper end of the channel body 1131 cannot detect cuvette 10, the cuvette arranging device 111 operates normally, and the cuvette 10 is supplied to the channel body 1131 through the slideway 112 .

As shown in Figure 12, the cuvette clamping arm 12 includes a radial support base 121, a synchronous pulley support base 122, a clamping synchronous pulley 123, a clamping guide rail 124 and a clamping portion 125, and the radial support base 121 is installed on On the system base plate 01, a synchronous pulley support seat 122 is installed on the radial support seat 121, and a holding synchronous pulley 123 rotating around the axis is installed on the upper end of the synchronous pulley support seat 122. The clamping guide rail 124 is slidably connected along the Z-axis direction, and the upper end of the clamping guide rail 124 is fixedly mounted with a clamping portion 125 .

The radial synchronous belt 126 is fixedly installed on the system base plate 01, and the radial support seat 121 is fixedly installed on the radial synchronous belt 126. The system base plate 01 drives the radial support seat 121 to move linearly along the system base plate 01 through the radial synchronous belt 126. .

A clamping Z-axis timing belt 127 is fixedly installed on the radial support base 121, and a clamping Z-axis support base 120 is fixedly installed on the clamping Z-axis timing belt 127, and the lower end of the clamping guide rail 124 rotates with the clamping Z-axis support base 120. Connected, the radial support base 121 drives the clamping Z-axis support base 120 to move along the Z-axis direction by clamping the Z-axis synchronous belt 127 .

A clamping and rotating synchronous belt 128 is fixedly installed on the synchronous pulley support seat 122, and the clamping and rotating synchronous belt 123 is meshed and connected to the inner side of the clamping and rotating synchronous belt 128. The synchronous pulley 123 rotates, and the clamping synchronous pulley 123 drives the clamping part 125 to rotate through the clamping guide rail 124 .

When in use, the clamping part 125 realizes the linear movement along the radial direction and the Z-axis direction of the reaction turntable 8 through the radial synchronous belt 126, the clamping Z-axis synchronous belt 127 and the clamping rotary synchronous belt 128 and the clamping synchronous belt The wheel 123 rotates about its axis.

As shown in Figure 13, the clamping part 125 includes a clamping support part 1251, a clamping housing 1252, a telescopic electromagnet 1253, an upper rack 1254, a lower rack 1255, an external gear 1256 and a clamping bar 1257, and the clamping support Part 1251 is fixedly connected with the top of guide rail 124, clamping housing 1252 is fixedly installed on clamping support part 1251, telescopic electromagnet 1253 is fixedly installed on clamping support part 1251, and the top end of telescopic rod of telescopic electromagnet 1253 is fixedly installed with upper Rack 1254, the upper rack 1254 is located at the upper end of the clamping housing 1252, the lower end of the clamping housing 1252 is equipped with a lower rack 1255 that is slidingly connected, and the outer gear 1256 is installed between the upper rack 1254 and the lower rack 1255 , the upper rack 1254 and the lower rack 1255 are meshed with the external gear 1256, and the lower ends of the upper rack 1254 and the lower rack 1255 are fixedly equipped with a clamping bar 1257.

The lower end of the clamping bar 1257 extends to the outside of the clamping shell 1252 .

When in use, start the telescopic electromagnet 1253, the telescopic end of the telescopic electromagnet 1253 drives the upper rack 1254 to move, and the upper rack 1254 moves through motion transmission, driving the lower rack 1255 to move, so that the clamping bars 1257 are separated from each other or clamped , to clamp or release the cuvette 10 placed between the clamping bars 1257 .

As shown in Figure 14, the cleaning station assembly 13 includes a magnetic separation base 131, a cleaning turntable 132, a suction needle 133, and a washing needle 134 (wherein the magnetic separation base 131 and the cleaning turntable 132 are respectively a kind of luminescence measurement of the patent application number CN201410856244.X The magnetic separation seat and the cleaning turntable disclosed in the instrument cuvette and its corresponding cleaning and separation mechanism), the magnetic separation seat 131 is equipped with a cleaning turntable 132 rotating around the axis, and the cleaning turntable 132 is provided with cuvette grooves distributed axially. 9. A needle-absorbing support 135 is fixedly installed on the magnetic separation base 131. The needle-absorbing support 135 is equipped with a needle-absorbing support plate 136 that moves along the Z-axis direction. Needle washing 134 is installed on the magnetic separation base 131 directly below the needle 133 , and the needle washing 134 is fixedly installed on the magnetic separation base 131 through the needle washing bracket 137 .

The cuvette clamping arm 12 is located between the reaction turntable 8 and the magnetic separation base 131 and between the magnetic separation base 131 and the measurement darkroom assembly 17 .

When the incubation time of a certain cuvette 10 reaches the specified time, turn the reaction turntable 8 to a specific position, and move it to the cuvette groove 9 of the cleaning turntable 132 through the cuvette clamping arm 12 .

When the cuvette 10 containing the reactant is placed in the cuvette groove 9 of the cleaning turntable 132, the cleaning turntable 132 rotates according to a certain time period, and then reaches the suction needle 133 and the washing needle 134 through the separation magnet in the magnetic separation seat 131 successively. At the bottom, the suction needle 133 first draws away the liquid in the cuvette 10 through the needle pump, and the washing needle 134 injects the washing solution into the cuvette 10 through the needle washing pump, and then the cleaning turntable 132 continues to rotate according to a certain period of time , when the cuvette 10 reaches the last suction needle 133, the magnetic beads move from one side of the cup to the other for 4 times, and the cuvette 10 is cleaned 4 times, thus ensuring the reliability and repeatability of the measurement results , and reduce the number of cleanings and speed up the test.

The heat preservation disk 14 is fixedly installed on the upper end of the system base plate 01, and the heat preservation disk 14 is located inside the cleaning station assembly 13. As shown in Figure 15, the heat preservation disk 14 is equipped with an axially rotating heat preservation turntable 15, and the heat preservation turntable 15 is provided with axially distributed The reaction cup groove 9 of the reaction cup groove 9, the heat preservation plate 14 heats the reaction cup 10 in the reaction cup groove 9 on the heat preservation turntable 15.

The substrate needle 16 is fixedly installed on the heat preservation tray 14 through the substrate needle support 161 .

After the reaction cup 10 is cleaned, if it is a one-step method, after the cleaning is completed, the reaction cup 10 is moved to the reaction cup groove 9 of the heat preservation turntable 15 by the reaction cup clamping arm 12, and the substrate pump makes the substrate needle 16 selectively Substrate is added to the cuvette 10 , and the heat preservation turntable 15 is rotated until the cuvette 10 is located under the cuvette clamping arm 12 next to the measurement darkroom assembly 17 .

For the two-step method or three-step method of some reagents, after cleaning, the cuvette clamping arm 12 does not move the cuvette 10 to the cuvette groove 9 of the heat preservation turntable 15, but returns the cuvette 10 to the reaction turntable 8. Carry out the reagent or sample loading and incubation process again, and the subsequent procedures are the same as the one-step method.

When the cuvette 10 on the heat preservation turntable 15 rotates to below the cuvette clamping arm 12 next to the measurement darkroom assembly 17, the cuvette 10 is moved to the measurement darkroom assembly 17 by the cuvette clamping arm 12 (such as Patent No. CN201310312544.7 In the drawer cup groove of the measurement darkroom assembly disclosed in a fully automatic biochemical and luminescence immunoassay system), the drawer is closed, the measurement darkroom assembly 17 is sealed, and at the same time, the light shielding plate in the measurement darkroom assembly 17 is opened, and the cuvette 10 is located in the photon Before the photodetector of the measurement component 18 (such as the photon measurement component disclosed in a fully automatic biochemical and luminescence immunoassay system of Patent No. CN201310312544.7), the photon measurement component 18 measures the luminescent product in the cuvette 10, and the computer Then calculate the concentration of the analyte according to the measurement results.

When the cuvette clamping arm 12 moves the cuvette 10 to the cuvette groove 9 of the heat preservation turntable 15, if it is the measurement requirement of the flash method, quickly rotate the heat preservation turntable 15 until the cuvette 10 is positioned at the cuvette holder next to the measurement darkroom assembly 17. Below the holding arm 12, the cuvette clamping arm 12 moves the cuvette 10 to the measurement darkroom assembly 17, the substrate needle in the darkroom assembly 17 adds the substrate to the cuvette 10, and the photon measurement assembly 18 reacts to the luminescence in the cuvette 10. The product is measured, and the computer calculates the concentration of the analyte according to the measurement result.

If it is the measurement requirement of the glow method, control the rotation speed of the heat preservation turntable 15 to delay and heat the cuvette 10 according to the demand. At this time, the addition of the substrate to the cuvette 10 is not limited to the substrate needle 16 located on the heat preservation plate 14. The substrate is added to the cuvette 10 , and the substrate can also be added to the cuvette 10 by measuring the substrate needle in the darkroom assembly 17 .

After the measurement, the drawer is opened, and the cuvette holding arm 12 moves the cuvette 10 into the waste cup tank, the waste liquid pump sucks the waste liquid away through the waste liquid needle group, and the discarded cuvette 10 falls into the waste cuvette channel , thus leading to the outside of the instrument.

In the description of this specification, descriptions with reference to the terms "one embodiment", "example", "specific example" and the like mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment of the present invention. In an embodiment or example. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The basic principles, main features and advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements are possible, which fall within the scope of the claimed invention.

Claims (10)

1. A high-throughput automatic luminescent immunoassay system, including a sample loading rack (2), a sample loading arm (3), a sample injection needle (4), a reagent chamber (5), a sample chamber (6), and a reaction incubation plate (7), Reaction Turntable (8), Reaction Cup Slot (9), Reaction Cup (10), Reaction Cup Automatic Filling Assembly (11), Reaction Cup Clamping Arm (12), Cleaning Station Assembly (13), Thermal Insulation Disk (14), heat preservation turntable (15), substrate needle (16), measurement darkroom assembly (17) and photon measurement assembly (18), reaction incubator disk (7) is equipped with axially rotating reaction turntable (8), The reaction cup automatic cup loading assembly (11) is located above the reaction turntable (8), and the photon measurement assembly (18) is fixedly installed in one side of the measurement darkroom assembly (17). It is characterized in that the sample loading bracket (2) Distributed on the side of the reaction turntable (8), the sample loading bracket (2) is equipped with a sample loading arm (3), and a sample loading needle (4) is fixedly installed on the sample loading arm (3); Reaction cup clamping arms (12) are installed between the reaction turntable (8) and the cleaning station assembly (13) and between the cleaning station assembly (13) and the measurement darkroom assembly (17); The heat preservation disc (14) is located inside the cleaning station assembly 13, the heat preservation turntable (15) which rotates axially is installed on the heat preservation disc (14), and the substrate needle (16) is fixedly mounted on the heat preservation plate (161) by the substrate needle support (161). on the plate (14). 2. A high-throughput automatic immunoluminescence analysis system according to claim 1, characterized in that, the reagent chamber (5) and the sample chamber (6) are respectively located on both sides of the reaction turntable (8). 3. A high-throughput automatic immunoluminescence analysis system according to claim 1, characterized in that, the reaction turntable (8) is provided with double rows of reaction cup grooves (9) distributed axially. 4. A high-throughput fully automatic immunoluminescence analysis system according to claim 1, characterized in that, the cuvette clamping arm (12) rotates along the radial direction and Z-axis direction of the reaction turntable (8). sports. 5. A high-throughput automatic immunoluminescence analysis system according to claim 1, characterized in that, the sample loading arm (3) moves along the X-axis direction, the Z-axis direction and rotates. 6. A high-throughput fully automatic immunoluminescence analysis system according to claim 2, characterized in that, the sample loading arm (3) is divided into two groups, the sample loading arm (3) on one side of the reagent chamber (5) 3) is a reagent arm, and the sample loading arm (3) located on one side of the sample chamber (6) is a sample arm. 7. A high-throughput fully automatic immunoluminescence analysis system according to claim 6, wherein there is a time interval between the movement of the sample arms; There is a time interval between the movements of the reagent arms. 8. A high-throughput fully automatic immunoluminescence analysis system according to claim 1, characterized in that, the cuvette automatic cupping assembly (11) is connected to the cuvette grooves (9) of the inner and outer rings of the reaction turntable (8) ) for automatic cup filling. 9. A high-throughput fully automatic immunoluminescence analysis system according to claim 6, characterized in that, the test tube rack delivery system (61) transports the test tube rack to the bottom of the corresponding sample arm, and the kit delivery system ( 55) Transport the reagent kit to the lower part of the corresponding reagent arm. 10. A high-throughput automatic immunoluminescence analysis system according to claim 9, characterized in that, the reagent box delivery system (55) arranges the reagent boxes in the reagent compartment (5).