CN110161003A - Optical detection apparatus and real time fluorescent quantitative nucleic acid amplification detection system - Google Patents
Optical detection apparatus and real time fluorescent quantitative nucleic acid amplification detection system Download PDFInfo
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- CN110161003A CN110161003A CN201910409817.7A CN201910409817A CN110161003A CN 110161003 A CN110161003 A CN 110161003A CN 201910409817 A CN201910409817 A CN 201910409817A CN 110161003 A CN110161003 A CN 110161003A
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Abstract
A kind of optical detection apparatus and real time fluorescent quantitative nucleic acid amplification detection system, optical detection apparatus includes actuator, multiple detection cartridges and multiple optical detecting modules, the actuator is used to for the reaction chamber of the single detection cartridge being moved to the light detection mouth out of optical detecting module described in any one, so that the optical detecting module carries out optical detection to the detection cartridge.Above-mentioned optical detection apparatus, by the way that multiple optical detecting modules are arranged, in, multiple optical detecting modules can use different wavelength, so, when needing to detect detection cartridge using corresponding wavelength, the reaction chamber of the single detection cartridge is moved on the light detection mouth out of one of them optical detecting module by actuator, can need to carry out different wavelength detectings respectively to multiple detection cartridges according to detection；And then multiple wavelength detectings can be suitable for.
Description
Technical field
This application involves technical field of optical detection, more particularly to a kind of optical detection apparatus and real time fluorescent quantitative core
Sour augmentation detection system.
Background technique
Polymerase chain reaction (Polymerase Chain Reaction, PCR) is a kind of specific for amplifying amplification
The Protocols in Molecular Biology of DNA fragmentation, it is considered as the special DNA replication dna of in vitro, and the maximum feature of PCR is to incite somebody to action
Micro DNA is significantly increased.Round pcr main process is that the small disconnected Single-stranded DNA fragments synthesized by the mankind also referred to as draw
Object, in conjunction with the specific regiospecificity of template DNA, and then using four kinds of dNTP as substrate, by archaeal dna polymerase along primer and
3 ' ends of the double stranded section that template is formed start, and polymerization forms the process that DNA fragmentation realizes DNA amplification in vitro.PCR is mainly by becoming
Sexual stage (90 DEG C -96 DEG C), extends stage (70 DEG C -75 DEG C) three fundamental reaction step structures at annealing stage (25 DEG C -65 DEG C)
At.
Real-Time Fluorescent Quantitative PCR Technique (Real-time Quantitative PCR Detecting System,
It qPCR), is that corresponding fluorescent dye or fluorescence labeling probe are added on the basis of Standard PCR, by glimmering in PCR reaction process
Change in optical signal is measured in real time entire PCR process, the product after each PCR cycle is monitored with fluorescent chemical
The method of total amount, the method that quantitative analysis is carried out to DNA sequence dna specific in sample to be tested.Fluorescence quantitative PCR instrument is application
The reaction kit that qPCR technology is measured in real time generally is guaranteed instrument function by heat circulating system, fluorescence real-time detecting system
Energy.Fluorescence quantitative PCR instrument mainly detects optical module, circuit control module, calculating by heating refrigeration cycle module, week fluorescent
Machine and its processing software composition.Wherein heating refrigeration cycle module realizes PCR's by forms such as air bath, water-bath, metal baths
Temperature cycles；Week fluorescent inspection optics is surveyed module and is mainly made of light source part, optical path, photoelectric sensor, control section.
However, existing fluorescence quantitative PCR instrument in carrying out optical detection, typically adds optical filter using single light source
Multi-wavelength excitation fluorescence is solved the problems, such as with turntable, is then realized in the way of multiple optical fiber or mechanical arm to multiplicity
This fluorescent collecting.It is this to design an apparatus that structure is complicated, realize difficult, control object is more, and instrument is big and heavy.The optical filter service life
It is shorter, it is easy to happen damage multi fiber and is also easy to happen confusion.
Summary of the invention
Based on this, it is necessary to provide the optical detection dress that a kind of structure is relatively simple, can be suitable for multiple wavelength detectings
It sets and real time fluorescent quantitative nucleic acid amplification detection system.
A kind of optical detection apparatus, including actuator, multiple detection cartridges and multiple optical detecting modules, the actuator
For the reaction chamber of the single detection cartridge to be moved to the light detection mouth out of optical detecting module described in any one
On, so that the optical detecting module carries out optical detection to the detection cartridge.
The actuator includes rotating electric machine and turntable, the rotary shaft of the rotating electric machine in one of the embodiments,
The turntable is connected, multiple detection cartridges are distributed on the same circumference centered on the rotary shaft of the turntable,
The turntable under the drive of the rotary shaft of the rotating electric machine for rotating, by the reaction chamber of the single detection cartridge
It is moved to going out on light detection mouth for optical detecting module described in any one, so that the optical detecting module is to the detection
Cartridge carries out optical detection.
The actuator includes mechanical arm in one of the embodiments, and the mechanical arm is used for will the single detection
The reaction chamber of cartridge is moved on the light detection mouth out of optical detecting module described in any one, so that the optical detection
Module carries out optical detection to the detection cartridge.
Above-mentioned optical detection apparatus, by the way that multiple optical detecting modules are arranged, in, multiple optical detecting modules can be with
Using different wavelength, in this way, when needing to detect detection cartridge using corresponding wavelength, it will be single by actuator
The reaction chamber of the detection cartridge is moved on the light detection mouth out of one of them optical detecting module, can be according to inspection
Survey needs to carry out different wavelength detectings respectively to multiple detection cartridges；And then multiple wavelength detectings can be suitable for.Relative to
It is existing by single light source add optical filter and turntable carry out multi-wavelength detection in the way of, the optical detection apparatus of the application uses
Multiple optical detecting modules can respectively correspond different wavelength, and then overall structure is relatively simple, easy to accomplish.
A kind of optical detection apparatus, comprising: support component, actuator, reaction component and optical sensing module；
The support component includes top plate and bottom plate and connecting rod, and the top plate is connected and supported by the connecting rod
On the bottom plate, mounting hole and several optical through-holes are offered on the top plate, several optical through-holes surround the installation
Hole setting；
The actuator includes rotating electric machine, connecting shaft and turntable, and the rotating electric machine is set on the bottom plate, described
The first end of connecting shaft connects the rotary shaft of the rotating electric machine, and the second end of the connecting shaft passes through the mounting hole and connects
The rotary shaft of the turntable, the rotating electric machine drives the turntable to rotate by the connecting shaft；
The reaction component includes several detection cartridges, and each detection cartridge connects the turntable, and several inspections
Cartridge is surveyed to be centrosymmetric centered on the connecting shaft distribution；Several reaction chambers are offered in the detection cartridge, and each
The reaction chamber is located at using the center of the connecting shaft as on the circumference in the center of circle；
The optical sensing module includes several optical detecting modules, and each optical detecting module is all set in the top
In the one side of the bottom plate, the center of several optical through-holes is located at same using the connecting shaft as the circumference in the center of circle plate
On line；The center of each optical through-hole and the center of each reaction chamber are located on same circumference；Each optical detection
Module goes out light detection mouth and each optical through-hole one-to-one correspondence.
The optical detecting module includes light source control plate, diversing lens, shines and filter in one of the embodiments,
Piece, exit lens, receives optical filter, receiving lens and circuit board for receiving at dichroscope；Light is provided on the light source control plate
Source is provided with photoelectric sensor on the circuit board for receiving；
Light sequence dichroscope described in directive after diversing lens and emission filter that the light source issues, through institute
State exit lens described in directive after dichroscope reflects；It is anti-described in the optical through-hole directive via being passed through after the exit lens
Answer chamber；
Reactant in the reaction chamber is excited through the light of the light source issues fluorescence, described in the fluorescence sequence process
Dichroscope described in directive after optical through-hole and the exit lens is filtered via receiving described in directive after dichroscope reflection
Mating plate, the receiving lens described in directive after the reception optical filter, by the receiving lens by the fluorescent foci to described
On photoelectric sensor.
The support component further includes mounting plate and several support rods in one of the embodiments, and the mounting plate is logical
It crosses several support rods to connect and be mounted on the bottom plate, the rotating electric machine is fixed on the mounting plate.
Perforating is also provided on the mounting plate in one of the embodiments, the rotating electric machine is fixed on described
In the one side of the bottom plate, the rotary shaft of the rotating electric machine passes through the perforating and connects the connection mounting plate
Axis.
The detection cartridge is PCR micro-fluidic chip in one of the embodiments,.
It is opened up in one of the embodiments, in several reaction chambers along the side close to connecting shaft in the detection cartridge
There is ventilation hole.
Several chip mounting grooves are offered on the turntable in one of the embodiments, each detection cartridge is one by one
Correspondence is installed in each chip mounting groove.
A kind of real time fluorescent quantitative nucleic acid amplification detection system is filled including optical detection described in any embodiment as above
It sets
Above-mentioned optical detection apparatus, due to using multiple optical detecting modules, in, multiple optical detecting modules can
To use different wavelength, in this way, when needing to detect detection cartridge using corresponding wavelength, by controlling electric rotating
The reaction chamber of the single detection cartridge is moved on the light detection mouth out of one of them optical detecting module by machine, energy
It is enough to need to carry out different wavelength detectings respectively to multiple detection cartridges according to detection；And then multiple wavelength can be suitable for and examined
It surveys.Relative to it is existing by single light source add optical filter and turntable carry out multi-wavelength detection in the way of, the optics of the application is examined
It surveys device and uses multiple optical detecting modules, different wavelength can be respectively corresponded, and then overall structure is relatively simple, be easy real
It is existing.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the optical detection apparatus of an embodiment；
Fig. 2 is the structural schematic diagram of the top plate of the optical detection apparatus of an embodiment；
Fig. 3 is the structural schematic diagram of the optical detection apparatus of an embodiment；
Fig. 4 is the structural schematic diagram of the detection cartridge of the optical detection apparatus of an embodiment；
Fig. 5 is the structural schematic diagram of the optical detecting module of the optical detection apparatus of an embodiment.
Specific embodiment
The application in order to facilitate understanding, in order to make the above objects, features, and advantages of the present application more apparent, under
Face is described in detail in conjunction with specific embodiment of the attached drawing to the application.It is explained in the following description many details
In order to fully understand the application, the better embodiment of the application is given in attached drawing.But the application can be with many not
With form realize, however it is not limited to embodiments described herein.On the contrary, the purpose of providing these embodiments is that making
Disclosure of this application is understood more thorough and comprehensive.The application can be to be much different from other way described herein
Implement, those skilled in the art can do similar improvement without prejudice to the application intension, therefore the application not by
The limitation of following public specific embodiment.In addition, term " first ", " second " are used for description purposes only, and should not be understood as
Indication or suggestion relative importance or the quantity for implicitly indicating indicated technical characteristic." first ", " are defined as a result,
Two " feature can explicitly or implicitly include at least one of the features.In the description of the present application, the meaning of " plurality " is
At least two, such as two, three etc., unless otherwise specifically defined.In the description of the present application, the meaning of " several "
It is at least one, such as one, two etc., unless otherwise specifically defined.It should be noted that when element is referred to as
" being fixed on " another element, it can directly on the other element or there may also be elements placed in the middle.When a member
Part is considered as " connection " another element, it can be directly to another element or may be simultaneously present member placed in the middle
Part.Term as used herein "vertical", "horizontal", "left" and "right" and similar statement simply to illustrate that mesh
, it is not meant to be the only embodiment.Unless otherwise defined, all technical and scientific terms used herein and category
It is identical in the normally understood meaning of those skilled in the art of the application.Term used herein is intended merely to describe
The purpose of specific embodiment, it is not intended that in limitation the application.Term " and or " used herein includes one or more
Any and all combinations of a relevant listed item.
In one embodiment, a kind of optical detection apparatus, including actuator, multiple detection cartridges and multiple optical detection moulds
Block, the actuator are used to the reaction chamber of the single detection cartridge being moved to optical detecting module described in any one
Go out light detection mouth on so that the optical detecting module to the detection cartridge progress optical detection.Above-mentioned optical detection dress
It sets, by the way that multiple optical detecting modules are arranged, in, multiple optical detecting modules can use different wavelength, in this way, working as
When needing to detect detection cartridge using corresponding wavelength, by actuator by the reaction chamber of the single detection cartridge
It is moved to going out on light detection mouth for one of them optical detecting module, can be needed according to detection to multiple detection cartridges point
Different wavelength detectings is not carried out；And then multiple wavelength detectings can be suitable for.Add optical filtering using single light source relative to existing
Piece and turntable carry out the mode of multi-wavelength detection, and the optical detection apparatus of the application uses multiple optical detecting modules, can be with
Different wavelength is respectively corresponded, and then overall structure is relatively simple, it is easy to accomplish.For example, the actuator includes rotating electric machine
And turntable, turntable described in the rotation axis connection of the rotating electric machine, multiple detection cartridges be distributed in the turntable with institute
State on the same circumference centered on rotary shaft, the turntable for being rotated under the drive of the rotary shaft of the rotating electric machine, with
The reaction chamber of the single detection cartridge is moved on the light detection mouth out of optical detecting module described in any one, with
The optical detecting module is set to carry out optical detection to the detection cartridge.For example, the actuator includes mechanical arm, the machine
The light that goes out that tool arm is used to for the reaction chamber of the single detection cartridge being moved to optical detecting module described in any one is examined
It surveys on mouth, so that the optical detecting module carries out optical detection to the detection cartridge.
It should be noted that the detection cartridge of the application, cartridge can be will test in advance and carried out after qPCR reaction completes
It is detected, can also directly will test cartridge is preferably micro-fluidic chip, especially PCR micro-fluidic chip, after real time reaction
It is detected.
It should be noted that the object of the present invention is to provide a kind of PCR of simple, compact multi-wavelength multisample is glimmering in real time
Optical detection system, in other words, the optical detection apparatus that the PCR real-time fluorescence applied to multi-wavelength multisample detects, realization side
Formula is compact-sized, easily fabricated, and detection accuracy is high.The present invention utilizes distributed optical detecting module, rotary reaction
Chamber realizes the real-time detection to multi-wavelength multisample.The present invention is mainly that optical detecting parts are integrated into one individually
In module, optical detecting module is formed, then multiple optical detecting modules carry out distributed arrangement according to expected design, and sample is anti-
Region is answered, i.e., the reaction chamber in detection cartridge is it is anticipated that also carry out distributed arrangement.Multi-wavelength measurement can be with multiple
Optical module is detected, each sample conversion zone can move to the alignment of any one optical detecting module, thus
The multi-wavelength measurement that each conversion zone may be implemented, to realize the detection system of multisample multi-wavelength.Sample reaction zone
Domain is the reaction chamber detected in cartridge, it is preferred that for the reaction chamber on PCR micro-fluidic chip.Sample control section is main
It is by sample conversion zone, which can be circular distribution, be also possible to rectangular distribution, either
Sector distribution, it can be metal material, be also possible to plastic material；Motion control mechanism is actuator, movement control
Mechanism processed is mainly the form of rotating electric machine or mechanical arm.Such as if reaction chamber is the either fan-shaped distribution of circular distribution,
Can be rotating electric machine makes entire reaction chamber rotate to realize the multi-wavelength measurement of each chamber；If it is rectangular or other
The distribution of form can be such that reaction chamber arbitrarily moves in plane with the form of mechanical arm, realize that the multi-wavelength of each chamber is surveyed
Amount.Sample control section is to detect cartridge, it is preferred that detection cartridge is micro-fluidic chip, it is furthermore preferred that detection cartridge is
PCR micro-fluidic chip.Motion control mechanism is actuator.The present invention is easily achieved the multi-wavelength measurement to some sample,
It is also very simple to increase measurable wavelength in this system, as long as increasing an optical detecting module, scalability and compatibility
Property is fine, arbitrarily can need expansion optical detection module according to detection.The movement of sample areas is only with optical detecting module
Vertical, thus increase sample size or change the distribution form of sample areas, there will not be any influence to optical measurement, therefore
Arbitrary sample size can be increased, realize the measurement of multisample.
The application is continued with to be illustrated in such a way that actuator includes rotating electric machine and turntable.
In one embodiment, a kind of optical detection apparatus, comprising: support component, actuator, reaction component and optical detection group
Part；The support component includes top plate and bottom plate and connecting rod, and the top plate is connected and supported by the connecting rod at the bottom
On plate, mounting hole and several optical through-holes are offered on the top plate, several optical through-holes are arranged around the mounting hole；
The actuator includes rotating electric machine, connecting shaft and turntable, and the rotating electric machine is set on the bottom plate, the connecting shaft
First end connects the rotary shaft of the rotating electric machine, and the second end of the connecting shaft passes through the mounting hole and connects described turn
The rotary shaft of disk, the rotating electric machine drives the turntable to rotate by the connecting shaft；The reaction component includes several inspections
Cartridge is surveyed, each detection cartridge connects the turntable, and several detection cartridges are rounded centered on the connecting shaft
It is spaced apart；Several reaction chambers are offered in the detection cartridge, and several reaction chambers are positioned at same with the company
On circumference centered on spindle；The optical sensing module includes several optical detecting modules, each optical detecting module
The top plate is all set in the one side of the bottom plate, several optical through-holes, which are located at, same with the connecting shaft is
On the circumference of the heart；Each optical through-hole and each reaction chamber are located on same circumference；Each optical detecting module
Go out light detection mouth and each optical through-hole corresponds.For example, a kind of optical detection apparatus, comprising: support component, driving
Part, reaction component and optical sensing module；The support component includes top plate and bottom plate and connecting rod, and the top plate passes through described
Connecting rod connects and supports on the bottom plate, and mounting hole and several optical through-holes, several light are offered on the top plate
Through-hole is learned to be arranged around the mounting hole；The actuator includes rotating electric machine, connecting shaft and turntable, the rotating electric machine setting
In on the bottom plate, the first end of the connecting shaft connects the rotary shaft of the rotating electric machine, and the second end of the connecting shaft is worn
It crosses the mounting hole and connects the turntable, the rotary shaft of the rotating electric machine drives the turntable to turn by the connecting shaft
It is dynamic；The reaction component includes several detection cartridges, and each detection cartridge connects the turntable, and several detection cartridges
Be centrosymmetric distribution centered on the connecting shaft；Several reaction chambers are offered in the detection cartridge, and each described anti-
Chamber is answered to be located at using the center of the connecting shaft as on the circumference in the center of circle；The optical sensing module includes several optical detections
Module, each optical detecting module are all set in the top plate in the one side of the bottom plate, several optical through-holes
Center be located at it is same using the connecting shaft as on the circumference in the center of circle；The center of each optical through-hole and each reaction chamber
The center of room is located on same circumference；Each optical detecting module goes out one a pair of light detection mouth and each optical through-hole
It answers.For example, the center of detection cartridge can be understood as geometric center.
In order to further illustrate above-mentioned optical detection apparatus, another example is, referring to Fig. 1, optical detection apparatus packet
Include support component 100, actuator 200, reaction component 300 and optical sensing module 400；The support component 100 mainly provides
Integrated support and installation effect.The actuator 200 connects the support component 100, and the actuator is made for providing driving
With.The reaction component 300 connects the driving component 200, and the reaction component is used as biochemical reaction, for example, PCR reacts
It uses.The optical sensing module 400 connects the support component 100, and the optical sensing module 400 is used for reaction component
Reactant in 300 provides optical detection.
Support component 100 includes top plate 110, bottom plate 120 and connecting rod 130, and the top plate 110 passes through the connecting rod
130 connect and support on the bottom plate 120, and in other words, one end of connecting rod connects the top plate, and the other end of connecting rod connects
The bottom plate is connect, and top plate is located above bottom plate.For another example, several connecting rods are set, in a specific embodiment, four connections are set
Bar, four connecting rods are located at four corner locations of the bottom plate or the top plate.Referring to Fig. 2, the top plate
Mounting hole 111 and several optical through-holes 112 are offered on 110, several optical through-holes 112 are set around the mounting hole 111
It sets.The mounting hole is used to install drive shaft by actuator, specific in the present embodiment, for the connecting shaft in actuator.
The optical through-hole is used to carry out optics to the reactant in reaction component by the optical through-hole convenient for optical sensing module
Detection.
Incorporated by reference to Fig. 3 to Fig. 5, the actuator 200 includes rotating electric machine 210, connecting shaft 220 and turntable 230, the rotation
Rotating motor is set on the bottom plate 120, certainly, practices, rotating electric machine can also be arranged on the top plate, and needs
Technological means same as the present application or essentially identical is also understood on top plate it is noted that being set to.The company
The first end of spindle 220 connects the rotary shaft of the rotating electric machine 210, and the second end of the connecting shaft 220 passes through the installation
Hole 111 simultaneously connects the turntable 230, and the rotary shaft of the rotating electric machine 210 drives the turntable by the connecting shaft 220
230 rotations；In other words, the connecting shaft under the drive of the rotary shaft of the rotating electric machine for driving the turntable to rotate.
For preferably mounting rotary electric machine, for example, referring to Fig. 3, the support component 100 further includes mounting plate 140 and several
Strut 150, the mounting plate 140 is connected by several support rods 150 and is mounted on the bottom plate 120, and the peace
Between the bottom plate 120 and the top plate 110, the rotating electric machine 210 is fixed on the mounting plate 140 loading board 140.
For another example, perforating is also provided on the mounting plate 140, it is neighbouring described that the rotating electric machine 210 is fixed on the mounting plate 140
In the one side of bottom plate 120, the rotary shaft of the rotating electric machine 210 passes through the perforating and connects the connecting shaft 220.Such as
This, being capable of preferably mounting rotary electric machine.
Fig. 1 and Fig. 4 is please referred to, reaction component 300 includes several detection cartridges 310, and each detection cartridge 310 connects institute
Turntable 230 is stated, and several detection cartridges 310 are rounded centered on the connecting shaft 220 is spaced apart；The detection card
Offer several reaction chambers 311 on box 310, and several reaction chambers 311 are located at and same with the connecting shaft 220 are
On the circumference of the heart；It should be noted that detection cartridge is the prior art.For example, the detection cartridge is micro-fluidic chip.Example
Such as, the micro-fluidic chip is PCR micro-fluidic chip.How PCR micro-fluidic chip carries out realizing PCR reaction, please refers to existing
Technology, details are not described herein by the application.Each reaction chamber of micro-fluidic chip is respectively used to carry out PCR reaction.It needs to illustrate
It is, in the application as previously described centered on connecting shaft, it is understood that for centered on the pivot center of connecting shaft.Example
Such as, ventilation hole 312 is offered along the side close to connecting shaft in several reaction chambers on the micro-fluidic chip 310, in this way, logical
Setting ventilation hole is crossed, the heating and cooling that PCR reacts are controlled convenient for micro-fluidic chip.Connect to preferably install micro-fluidic chip
It connects on turntable, for example, offering several chip mounting grooves on the turntable, each micro-fluidic chip one-to-one correspondence is installed on
In each chip mounting groove.For example, the fan-shaped structure of micro-fluidic chip.For example, being also provided with gear on the micro-fluidic chip
Slot, the turntable are convexly equipped with block in the side wall of the mounting groove, and the block is corresponding with the retaining groove, by the miniflow
Chip limit is controlled in the mounting groove.
Referring to Fig. 3, optical sensing module 400 includes several optical detecting modules 410, each optical detecting module
410 are all set in the top plate 110 in the one side of the bottom plate 120, and several optical through-holes 112 are located at same with institute
It states on the circumference centered on connecting shaft 220；Each optical through-hole 112 is located at same circumference with each reaction chamber 311
On；Each optical detecting module 410 goes out light detection mouth and each one-to-one correspondence of optical through-hole 112, in other words, the light
The corresponding optical through-hole of light detection mouth that goes out for learning detection module aligns, in this way, going out light inspection convenient for optical detecting module
It surveys mouth and optical detection is carried out by reaction chamber of the optical through-hole to the detection cartridge.
Above-mentioned optical detection apparatus, due to using multiple optical detecting modules 410, in, multiple optical detection moulds
Block can use different wavelength, in this way, being revolved when needing to detect detection cartridge using corresponding wavelength by control
The reaction chamber of the single detection cartridge is moved to the light detection mouth out of one of them optical detecting module by rotating motor
On, it can need to carry out different wavelength detectings respectively to multiple detection cartridges according to detection；And then multiple waves can be suitable for
Long detection.Relative to it is existing by single light source add optical filter and turntable carry out multi-wavelength detection in the way of, the light of the application
It learns detection device and uses multiple optical detecting modules, different wavelength can be respectively corresponded, and then overall structure is relatively simple, hold
Easily realize.
In one of the embodiments, the optical detecting module 410 include light source control plate 411, diversing lens 412,
Emission filter 413, exit lens 415, receives optical filter 416, receiving lens 417 and circuit board for receiving at dichroscope 414
418；It is provided with light source on the light source control plate 411, is provided with photoelectric sensor on the circuit board for receiving 419；The light
Light sequence dichroscope 414 described in directive after diversing lens 412 and emission filter 413 that source issues, through described two
Exit lens 415 described in directive after being reflected to Look mirror 414；It is penetrated via the optical through-hole 112 is passed through after the exit lens 415
To the reaction chamber 311；
Reactant in the reaction chamber 311 issues fluorescence through the light excitation of the light source, and the fluorescence sequence is passed through
Dichroscope 414 described in directive after the optical through-hole 112 and the exit lens 415 is reflected via the dichroscope 414
Optical filter 416, the receiving lens 417 described in directive after the reception optical filter 417, by the reception are received described in directive afterwards
Lens 417 will be on the fluorescent foci to the photoelectric sensor.The photoelectric sensor carries out optics to the fluorescence received
Photosignal is analyzed and is converted into, corresponding data analysis result can be obtained by then analyzing photosignal.In this way,
Quantitative fluorescence analysis can preferably be carried out.
It should be noted that optical detecting module as noted earlier goes out light detection mouth, as exit lens 415 sides
Outlet.For another example, the optical detecting module 410 includes sleeve 410a, light source control plate 411, diversing lens 412, shines and filter
Piece 413, exit lens 415, receives optical filter 416, receiving lens 417 and circuit board for receiving 418 at dichroscope 414；The light
Source control panel 411, the diversing lens 412, the emission filter 413, the dichroscope 414, the exit lens
415, the reception optical filter 416, the receiving lens 417 and the circuit board for receiving 418 are all set in the sleeve 410
It is interior；The whole structure in T shape of the sleeve, or in similar T-shaped structure, the sleeve have first end, second end and the
The first end and second end at three ends, the sleeve is parallel, and the third end of the sleeve is respectively perpendicular to its first end and second end,
The dichroscope is located at the first end, second end and the link position at third end of the sleeve, the light source control plate
411, diversing lens 412 and emission filter 413 are located at the third end of the sleeve, and the exit lens 415 are located at the set
The first end of cylinder, reception optical filter 416, the receiving lens 417 and the circuit board for receiving 418 are located at the sleeve
Second end.It so, it is possible preferably to carry out optical detection.It should be noted that the first end of sleeve is it can be appreciated that outgoing
Optical path sleeve, the second end of sleeve is it can be appreciated that receiving light path sleeve, the third end of sleeve is it can be appreciated that transmitting light
Road sleeve.Transmitting optical path sleeve major function is the installation and fixation of LED light source and diversing lens and transmitting optical filter, light source
Control panel is also used to external control circuit.Emitting light path sleeve is mainly exit lens, guarantees that the luminous energy of light source focuses on reaction
Chamber.Receiving light path sleeve is mainly lens and optical filter, it is ensured that can reach reception circuit by the fluorescence of optical through-hole；It connects
Circuit board is received to be also used to connect with external signal Processing Interface.Light source control plate controls light-source brightness；Diversing lens are used
In making the light of light source become directional light by the lens, this directional light passes in exit lens using dichroscope, through being emitted
On lens focus to reaction chamber；Reactant in reaction chamber inspires fluorescence through the light of this light source, and the fluorescence is using out
It penetrates lens to get on dichroscope, fluorescence, by receiving optical filter, gets to receiving lens by dichroscope；This fluorescence passes through
Photoelectric sensor above lens focus to circuit board for receiving carries out the conversion of photosignal.
Above-mentioned optical detection apparatus, due to using multiple optical detecting modules 410, in, multiple optical detection moulds
Block can use different wavelength, in this way, being revolved when needing to detect detection cartridge using corresponding wavelength by control
The reaction chamber of the single detection cartridge is moved to the light detection mouth out of one of them optical detecting module by rotating motor
On, it can need to carry out different wavelength detectings respectively to multiple detection cartridges according to detection；And then multiple waves can be suitable for
Long detection.Relative to it is existing by single light source add optical filter and turntable carry out multi-wavelength detection in the way of, the light of the application
It learns detection device and uses multiple optical detecting modules, different wavelength can be respectively corresponded, and then overall structure is relatively simple, hold
Easily realize.
It should be noted that the object of the present invention is to provide a kind of PCR of simple, compact multi-wavelength multisample is glimmering in real time
Optical detection system, in other words, the optical detection apparatus that the PCR real-time fluorescence applied to multi-wavelength multisample detects, realization side
Formula is compact-sized, easily fabricated, and detection accuracy is high.The present invention utilizes distributed optical detecting module, rotary reaction
Chamber realizes the real-time detection to multi-wavelength multisample.The present invention is mainly that optical detecting parts are integrated into one individually
In module, optical detecting module is formed, then multiple optical detecting modules carry out distributed arrangement according to expected design, and sample is anti-
Region, i.e. reaction chamber on micro-fluidic chip are answered it is anticipated that also carrying out distributed arrangement.Multi-wavelength measurement can be used more
A optical module is detected, each sample conversion zone can move to the alignment of any one optical detecting module, from
And the multi-wavelength measurement of each conversion zone may be implemented, to realize the detection system of multisample multi-wavelength.Sample reaction
Reaction chamber on region, that is, micro-fluidic chip.
Mainly by sample conversion zone, which can be circular distribution for sample control section,
It is also possible to rectangular distribution, or fan-shaped distribution, it can be metal material, be also possible to plastic material；Movement
Control mechanism is actuator, and motion control mechanism is mainly the form of motor or mechanical arm.If such as reaction chamber is round
The either fan-shaped distribution of shape distribution, can be motor makes entire reaction chamber rotate to realize that the multi-wavelength of each chamber is surveyed
Amount；If rectangular or other forms distributions, reaction chamber can be made arbitrarily to move in plane with the form of mechanical arm, realized
The multi-wavelength of each chamber measures.Sample control section is micro-fluidic chip.Motion control mechanism is actuator.
The present invention is easily achieved the multi-wavelength measurement to some sample, to increase measurable wavelength also very in this system
Simply, as long as increasing an optical detecting module, scalability and compatibility are fine, arbitrarily can need to extend according to detection
Optical detecting module.
The movement of sample areas is independent with optical detecting module, thus increases sample size or change sample areas
Distribution form, will not have any influence to optical measurement, therefore arbitrary sample size can be increased, realize the survey of multisample
Amount.
In specific embodiment, when actuator uses rotating electric machine, rotating electric machine main function is rotation, drives several micro-
Fluidic chip rotates together；Optical detecting module is fixed on the underface of reaction chamber in micro-fluidic chip by top plate, passes through
The optical through-hole of hollow out on top plate carries out realization optical detection.When reaction chamber is rotated with motor, in micro-fluidic chip
Each reaction chamber can be successively by each optical module of lower section；It is logical to ensure that each sample may pass through optics
Road.
In one specific embodiment, reaction component includes 8 sample reaction modules, has several reactions on each reaction module
Chamber has ventilation hole conveniently to do PCR reaction in each module.Sample reaction module is micro-fluidic chip.
A kind of qPCR system that can be realized the measurement of multi-wavelength multisample is constituted described in above-mentioned.A kind of distributed optics
Measuring system, optical module fully functional by several, independent are arranged according to sample distribution, and can work independently can also combine work
The system of work.A kind of distribution of sample reaction chamber and its control system, ensure that each reaction chamber can by movement mechanism
It enough moves to each optical module and nearby carries out optical measurement.A kind of PCR reaction system, in conjunction with above-mentioned optical measuring system
With sample reaction chamber and its control system, realizes and the system of multisample, multi-wavelength was realized in PCR reaction process
Journey.
The present invention also provides a kind of real time fluorescent quantitative nucleic acid amplification detection systems, including described in any embodiment as above
Optical detection apparatus.
Above-mentioned real time fluorescent quantitative nucleic acid amplification detection system uses above-mentioned optical detection apparatus, by the way that multiple optics are arranged
Detection module, in, multiple optical detecting modules can use different wavelength, in this way, when needing using corresponding wavelength
When being detected to detection cartridge, the reaction chamber of the single detection cartridge is moved to described in one of them by actuator
Optical detecting module goes out on light detection mouth, can be needed to carry out multiple detection cartridges respectively different wavelength inspections according to detection
It surveys；And then multiple wavelength detectings can be suitable for.Using single light source add optical filter and turntable relative to existing and carry out more waves
The optical detection apparatus of the mode of long detection, the application uses multiple optical detecting modules, can respectively correspond different wavelength,
And then overall structure is relatively simple, it is easy to accomplish.
In order to further illustrate above-mentioned micro-fluidic chip；The application also provides a kind of micro-fluidic chip.For example, a kind of miniflow
Chip is controlled, micro-fluidic chip has rotation center, and micro-fluidic chip includes chip basal body, and is set to adding in chip basal body
Sample hole, feeding chamber, gas outlet, sample enrichment chamber, waste liquid chamber, dilution cracking chamber, siphon piping, feeding chamber circulation duct, cracking
Chamber circulation duct, gas flow pipe road, sample output channel, reagent distribution pipeline, outlet pipe and multiple reaction chambers；Waste liquid
Chamber includes the first waste liquid chamber and the second waste liquid chamber；Well is respectively communicated with external and feeding chamber, and feeding chamber passes through feeding chamber stream
It threads a pipe and is connected to sample enrichment chamber；Sample is enriched with chamber and is connected to the first waste liquid chamber by cracking chamber circulation duct, and sample is enriched with chamber also
Pass through sample output channel connection dilution cracking chamber in its bottom position far from rotation center；Dilution cracking chamber sequence passes through
Siphon piping and reagent distribution pipeline are respectively communicated with each reaction chamber and the second waste liquid chamber；First waste liquid chamber passes through gas flow pipe
Road is connected to the second waste liquid chamber；The external setting of gas outlet connection, gas outlet are also connected to a waste liquid chamber, and waste liquid chamber by outlet pipe
The position being connected with gas outlet compared with gas outlet more far from rotation center.Above-mentioned micro-fluidic chip is suitable for centrifugal microfluidic
Control analysis, dilutes after the enrichment of sample, cracking, cracking and the PCR amplification of equivalent distribution, multi-chamber is all able to sequence and realizes,
It can be realized and exempt from nucleic acid purification molecular diagnosis function, one side gas outlet is connected to a waste liquid chamber and is connected to by the waste liquid chamber another
Waste liquid chamber can adjust the position of gas outlet according to demand, and capillary force is dexterously on the other hand utilized by designing siphon piping
With the relativeness of centrifugal force, controlling valve is formd to control liquid and flow into reagent distribution pipeline, utilizes PCR amplification application
Realize that the molecular diagnosis of based on PCR amplification, entire reaction process are in closed micro-fluidic chip in centrifugal microfluidic control technology,
Realize the molecular diagnosis effect quickly detected whenever and wherever possible.Reaction chamber in the present embodiment is used for PCR amplification.Certainly it needs
It is noted that the micro-fluidic chip of the application is not limited to this, can also just there be the PCR for being able to carry out detection in the prior art
Micro-fluidic chip.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.It should be noted that " in an embodiment ", " example of the application
Such as ", " for another example ", it is intended to the application is illustrated, rather than for limiting the application.Embodiment described above only table
The several embodiments of the application are reached, the description thereof is more specific and detailed, and but it cannot be understood as to applying for a patent
The limitation of range.It should be pointed out that for those of ordinary skill in the art, in the premise for not departing from the application design
Under, various modifications and improvements can be made, these belong to the protection scope of the application.Therefore, the protection of the application patent
Range should be determined by the appended claims.
Claims (10)
1. a kind of optical detection apparatus, which is characterized in that including actuator, multiple detection cartridges and multiple optical detecting modules,
The actuator is used to the reaction chamber of the single detection cartridge being moved to optical detecting module described in any one
Out on light detection mouth, so that the optical detecting module carries out optical detection to the detection cartridge.
2. optical detection apparatus according to claim 1, which is characterized in that the actuator includes rotating electric machine and turns
Disk, turntable described in the rotation axis connection of the rotating electric machine, multiple detection cartridges be distributed in the turntable with the rotation
On same circumference centered on shaft, the turntable, will be single for rotating under the drive of the rotary shaft of the rotating electric machine
The reaction chamber of a detection cartridge is moved on the light detection mouth out of optical detecting module described in any one, so that institute
It states optical detecting module and optical detection is carried out to the detection cartridge.
3. optical detection apparatus according to claim 1, which is characterized in that the actuator includes mechanical arm, the machine
The light that goes out that tool arm is used to for the reaction chamber of the single detection cartridge being moved to optical detecting module described in any one is examined
It surveys on mouth, so that the optical detecting module carries out optical detection to the detection cartridge.
4. a kind of optical detection apparatus characterized by comprising
Support component, the support component include top plate and bottom plate and connecting rod, and the top plate is connected and propped up by the connecting rod
Support offers mounting hole and several optical through-holes, several optical through-holes are around described on the bottom plate on the top plate
Mounting hole setting；
Actuator, the actuator include rotating electric machine, connecting shaft and turntable, and the rotating electric machine is set on the bottom plate,
The first end of the connecting shaft connects the rotary shaft of the rotating electric machine, and the second end of the connecting shaft passes through the mounting hole simultaneously
The turntable is connected, the rotary shaft of the rotating electric machine drives the turntable to rotate by the connecting shaft；
Reaction component, the reaction component include several detection cartridges, and each detection cartridge connects the turntable, and several institutes
Detection cartridge is stated to be centrosymmetric distribution with the center of the connecting shaft；Several reaction chambers are offered in the detection cartridge,
And the center of each reaction chamber is located at using the center of the connecting shaft as on the circumference in the center of circle；
Optical sensing module, the optical sensing module include several optical detecting modules, and each optical detecting module is all provided with
The top plate is placed in the one side of the bottom plate, the centers of several optical through-holes is located at same with the connecting shaft
Center is on the circumference in the center of circle；The center of each optical through-hole and the center of each reaction chamber are located at same circumference
On；Each optical detecting module goes out light detection mouth and each optical through-hole one-to-one correspondence.
5. optical detection apparatus according to claim 4, which is characterized in that the optical detecting module includes light source control
Plate, emission filter, dichroscope, exit lens, receives optical filter, receiving lens and circuit board for receiving at diversing lens；It is described
It is provided with light source on light source control plate, is provided with photoelectric sensor on the circuit board for receiving；
Light sequence dichroscope described in directive after diversing lens and emission filter that the light source issues, through described two
Exit lens described in directive after to Look mirror reflection, by being passed through described in the corresponding optical through-hole directive after the exit lens
Reaction chamber；
Reactant in the reaction chamber issues fluorescence through the light excitation of the light source, and the fluorescence sequence passes through the optics
Dichroscope described in directive after through-hole and the exit lens filters via receiving described in directive after dichroscope reflection
Piece, the receiving lens described in directive after the reception optical filter, by the receiving lens by the fluorescent foci to the light
On electric transducer.
6. optical detection apparatus according to claim 4, which is characterized in that if the support component further include mounting plate and
Heavenly Stems and Earthly Branches strut, the mounting plate are connected and are mounted on the bottom plate by several support rods, and the rotating electric machine is fixed
In on the mounting plate.
7. optical detection apparatus according to claim 6, which is characterized in that it is also provided with perforating on the mounting plate,
The rotating electric machine is fixed on the mounting plate in the one side of the bottom plate, and the rotary shaft of the rotating electric machine passes through described
Perforating simultaneously connects the connecting shaft.
8. optical detection apparatus according to claim 4, which is characterized in that the detection cartridge is PCR micro-fluidic chip.
9. optical detection apparatus according to claim 8, which is characterized in that in several reaction chambers in the detection cartridge
Ventilation hole is offered along the side close to connecting shaft；
And/or several chip mounting grooves are offered on the turntable, each detection cartridge one-to-one correspondence is installed on each core
In piece mounting groove.
10. a kind of real time fluorescent quantitative nucleic acid amplification detection system, which is characterized in that including in such as any one of claim 1 to 9
The optical detection apparatus.
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