CN113583854A - Nucleic acid detection device capable of realizing household self-detection, detection method and application - Google Patents
Nucleic acid detection device capable of realizing household self-detection, detection method and application Download PDFInfo
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Abstract
The invention provides a nucleic acid detection device capable of realizing household self-detection, a detection method and application, wherein the nucleic acid detection device comprises a shell, a circuit board is arranged in the shell, an amplification plate is arranged in the central area of the circuit board, at least one amplification cabin is arranged on the outer edge of the amplification plate along the circumferential direction, a liquid inlet cabin is arranged in the center of the amplification plate, and the liquid inlet cabins are independently communicated with the amplification cabins; the periphery of the amplification plate is provided with a detection light source; one end of the shell is inserted into a needle tube, and one end of the needle tube extending into the shell is butted with the liquid inlet cabin; the nucleic acid detecting device further comprises a reagent tube. The nucleic acid detection device provided by the invention adopts a visible light detection mode, and a complex light path system required by fluorescence detection is omitted, so that the device can be miniaturized and lightened, the operation process is simplified, the operation difficulty and the detection threshold are reduced, and the device is particularly suitable for the real-time detection of nucleic acid samples in public occasions such as customs, families, schools and the like.
Description
Technical Field
The invention belongs to the technical field of nucleic acid detection, and relates to a nucleic acid detection device capable of realizing household self-detection, a detection method and application.
Background
Polymerase Chain Reaction (PCR), also known as cell-free molecular cloning or specific DNA sequence in vitro primer directed enzymatic amplification technology, is a method for synthesizing specific DNA fragments in vitro enzymatically, and a cycle is formed by several steps of reactions such as high-temperature denaturation, low-temperature annealing, suitable temperature extension and the like, is performed in a circulating manner, so that the target DNA can be rapidly amplified, and the method has the characteristics of strong specificity, high sensitivity, simplicity and convenience in operation, time saving and the like. It can be used for basic research of gene separation, cloning and nucleic acid sequence analysis, etc. and also for diagnosis of diseases, fast detection of animal virus and epidemic situation or any place with DNA and RNA. In clinical medicine and animal epidemic monitoring systems, PCR is used to identify genetic diseases and to rapidly detect viral and bacterial infections. The traditional method needs to culture the pathogen for several weeks to identify the virus and germ infection, and the invention can quickly judge whether the DNA of the virus and germ exists in the human body or animal cells (such as blood cells) to confirm the diagnosis.
CN212514273U discloses PCR sampling check out test set, including the detection box, the prefabricated pipe of PCR reaction, the fluorescence probe, heating device and sample thief, detection box upper end opening, be provided with the support ring in the upper end, the prefabricated pipe fixed connection of PCR reaction is on the support ring and the through-hole at middle part lower extrusion support ring center, fluorescence probe fixed connection just is just to the prefabricated intraductal convex part down of PCR reaction at the lower terminal surface of support ring, heating device is installed to the prefabricated intraductal below of PCR reaction, heating device fixed connection is in the detection box, the sample thief can cover and close the detection box upper end and can squeeze the liquid on the sampling test paper into in the prefabricated pipe of PCR reaction.
CN212800301U discloses a portable PCR detection device, including detecting box, the prefabricated pipe of PCR reaction, fluorescence probe and heating device, detect box upper end opening is provided with the support ring in the upper end, the prefabricated pipe fixed connection of PCR reaction on the support ring and the through-hole at middle part lower extrusion support ring center, fluorescence probe fixed connection just is just to the prefabricated intraductal convex part down of PCR reaction at the lower terminal surface of support ring, heating device is installed to the prefabricated pipe middle part below of PCR reaction, heating device fixed connection is in detecting the box.
CN212713554U discloses a virus PCR short-term test device, including the packing carton, the upper cover plate, reagent tube base, reagent tube hole groove, upper cover heating module and base heating module, reagent tube base installs in the packing carton, the vertical multirow reagent tube hole groove that is provided with on the reagent tube base, the rear portion of packing carton can open and shut be connected with the upper cover plate, the upper cover plate lid closes and forms a confined box body on the packing carton after, upper cover heating module is installed to the lower part in the upper cover plate, it all installs base heating module to lie in every cavity in the reagent tube base. Install base temperature sensor on the reagent tube base, upper cover temperature sensor is installed to the upper cover plate lower extreme, and liquid crystal display and operation button are installed on the upper cover plate top, and liquid crystal display, upper cover temperature sensor, base temperature sensor, operation button all are connected with the main control board through the wire.
The existing nucleic acid detection device is complex in operation, is not suitable for home self-detection, and cannot visually obtain a detection result, so that a nucleic acid detection device suitable for home self-detection needs to be designed.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a nucleic acid detection device capable of realizing household self-detection, a detection method and application.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a nucleic acid detection device capable of realizing household self-test, which comprises a shell, wherein a circuit board is arranged in the shell, an amplification board and a detection light source are arranged on the circuit board, at least one amplification cabin is arranged on the amplification board, one end of the shell is inserted into a needle tube, and one end of the needle tube, which extends into the shell, is butted with the amplification cabin;
the nucleic acid detection device also comprises a reagent tube which is penetrated through the needle tube, and reactants in the reagent tube flow into the amplification cabin through the reagent tube to carry out nucleic acid amplification.
The nucleic acid detection device provided by the invention adopts a visible light detection mode, and a complex light path system required by fluorescence detection is omitted, so that the device can be miniaturized and lightened, the operation process is simplified, the operation difficulty and the detection threshold are reduced, and the device is particularly suitable for the real-time detection of nucleic acid samples in public occasions such as customs, families, schools and the like.
In a preferred embodiment of the present invention, the amplification plate is disposed in a central region of the circuit board.
Preferably, at least one amplification cabin is arranged on the outer edge of the amplification plate along the circumferential direction.
Preferably, the center department of amplification board has still seted up into the liquid cabin, it independently communicates each amplification cabin to advance the liquid cabin, the needle tubing stretches into the one end and the feed liquor cabin intercommunication of casing, and reaction liquid flows into the liquid cabin by the needle tubing to the liquid cabin reposition of redundant personnel gets into each amplification cabin through the feed liquor.
Preferably, the detection light source is arranged on the periphery of the amplification plate and is positioned on the circuit board.
Preferably, the detection light source is a visible light source.
As a preferred technical scheme of the invention, the circuit board comprises an upper circuit board and a lower circuit board which are electrically connected in an inserting manner, and the amplification board is positioned on the upper circuit board.
Preferably, the periphery of the amplification plate is provided with the same number of detection light sources as the amplification cabins, and each detection light source corresponds to one amplification cabin.
As a preferred technical solution of the present invention, the upper circuit board is provided with a driving motor, the driving motor is electrically connected to the amplification plate, and the driving motor is used for driving the bottom plate to intermittently rotate.
Preferably, the periphery of the amplification plate is provided with a detection light source, during the period of stopping rotation, the detection light source corresponds to one of the amplification cabins, and the detection light source is switched to the next amplification cabin after rotating once.
It should be noted that there are two different detection schemes of the present invention, specifically:
the first scheme is as follows: when the amplification plate is detected in a fixed mode, a plurality of detection light sources are required to be arranged on the periphery of the amplification plate, each detection light source corresponds to one amplification cabin, different amplification reagents are put into different amplification cabins in advance, reactants in reagent tubes flow into different amplification cabins through liquid inlet cabins respectively to carry out amplification reaction, and after the amplification reaction is finished, the detection light sources are started to irradiate the corresponding amplification cabins during detection. Although this solution requires a plurality of detection light sources, it has an advantage that different detection means can be performed on the sample to be detected at the same time.
Scheme II: when the amplification plate is detected in a rotating mode, only one detection light source is arranged on the periphery of the amplification plate, the amplification plate is driven to rotate by a driving motor, reactants in a reagent tube respectively flow into different amplification cabins through liquid inlet cabins in an initial state, different amplification reagents are put into different amplification cabins in advance, the detection light source is aligned to one of the amplification cabins after amplification is finished and detection is started, light beams emitted by the detection light source irradiate the reactants in the amplification cabins, and the detection result of a detection sample in the amplification cabins is obtained through color detection; and after the detection is finished, rotating the amplification plate, switching to the next amplification cabin, repeating the steps, and detecting the samples to be detected in each amplification cabin one by one. The scheme saves the cost of redundant detection light sources, but only can carry out a detection means on the sample to be detected at the same time.
As a preferred technical scheme of the present invention, the detection light source is covered and buckled with a reflector with a single side opening, the opening of the reflector faces the amplification chamber, and light beams emitted by the detection light source are reflected and gathered by the reflector and then irradiate to the corresponding amplification chamber.
Preferably, the reflector is fixed on the circuit board.
Preferably, the fixing mode is welding fixing.
As a preferable technical scheme of the invention, a heating film is arranged between the amplification plate and the circuit board and used for heating or cooling reactants in the amplification cabin.
Preferably, the heating film is of a ring structure.
Preferably, a color sensor is arranged between the amplification board and the circuit board, the color sensor is electrically connected with the circuit board, the detection light source, the amplification cabin and the color sensor are positioned on the same light path, and light beams emitted by the detection light source are received by the color sensor after passing through reactants in the amplification cabin.
Preferably, the color sensor is located at the central hollow of the annular heating film.
Preferably, a light-transmitting plate is further arranged between the amplification plate and the circuit board, a light path channel is formed in the light-transmitting plate, the detection light source, the amplification cabin, the light path channel and the color sensor are located on the same light path, and light beams emitted by the detection light source penetrate through the amplification cabin and are introduced into the color sensor through the light path channel.
Preferably, the light-transmitting plate is located at a hollow-out position in the center of the annular heating film.
The invention aims to provide a light-transmitting plate, which comprises the following components: (1) gathering light beams emitted by different amplification cabins, and improving the detection precision; (2) light beams emitted by different amplification cabins are distinguished, and the mutual influence of different light beams is avoided, so that result misjudgment is caused.
As a preferable technical scheme of the invention, an upper cover plate is further arranged in the shell, and the upper cover plate and the amplification plate are buckled with each other.
Preferably, a sealing film is arranged on the joint surface of the upper cover plate and the amplification plate.
The sealing film of the present invention is made of an elastic material, and the elastic material is required to have the characteristics of hydrophobicity, no adsorption of nucleic acid, high temperature resistance, etc., and the specific type of the elastic material is not required or limited. The sealing film is arranged between the upper cover plate and the amplification plate, and when the upper cover plate is mutually buckled with the amplification plate, the sealing effect between the upper cover plate and the amplification plate is improved through the sealing film.
Preferably, the surface of the upper cover plate is provided with a liquid inlet, one end of the needle tube is inserted into the liquid inlet, and when the detection is started, the needle tube penetrates through the liquid inlet cabin and pierces the sealing film to extend into the liquid inlet cabin.
Preferably, a baffle is arranged at one end of the needle tube far away from the upper cover plate, and a sealing piece is arranged on the periphery of the needle tube.
The sealing element provided by the invention is of a jacket structure, elastic materials are filled in the jacket, and the sealing element is mainly used for sealing the upper cover plate, so that the whole nucleic acid detection device can only be contacted with the external environment through the needle tube.
Preferably, the shell is provided with an indicator light array, the circuit board is provided with a data processor in an integrated manner, the data processor is respectively and independently electrically connected with the indicator light array and the color sensor, the color sensor transmits an optical signal to the data processor, and the data processor instructs the indicator light array to display a positive or negative detection result according to the optical signal.
The invention realizes the qualitative analysis of a sample to be detected and outputs the detection result quickly and intuitively through the detection logic formed by the detection light source, the color sensor, the data processor and the indicator light array, the detection light source emits visible light and irradiates on a reactant, an optical signal emitted by the reactant after irradiation is received by the color sensor and then transmitted to the data processor, the detection result is obtained in the data processor through the optical signal, and the indicator light array is controlled to display positive/negative according to the detection result.
It should be noted that the present invention can simultaneously perform different amplification reactions (different amplification reagents are placed in the amplification chamber in advance) on a sample to be tested, thereby detecting positive/negative results of different diseases. Each sample to be detected corresponds to one indicator light or one group of indicator lights, and the data processor controls the corresponding indicator lights to change according to the detection results of different samples, the color change mode of the indicator lights is not specifically required and specially limited, in principle, a detector can determine the detection result through the change of the indicator lights, and exemplarily, the following indication modes can be adopted to display the detection result:
the first scheme is as follows: the detection result is indicated by means of the on/off or color change of a single lamp, for example: when the indicator lamp is turned on, the corresponding sample to be detected is positive, and when the indicator lamp is kept off, the corresponding sample to be detected is negative; of course, the detection result can also be indicated in a color change mode.
Scheme II: the two indicating lamps are a group of indicating detection results, one indicating lamp is a positive indicating lamp, the other indicating lamp is a negative indicating lamp, if the positive indicating lamp is lighted, the sample to be detected is positive, and if the negative indicating lamp is lighted, the sample to be detected is negative.
Further, the present invention may also adopt other result display modes, such as: and the remote display of the result is realized through a wireless transmission technology. The specific scheme can be selected as follows: set up wireless transmission module in the casing, data processor and wireless transmission module electric connection, wireless transmission module and mobile terminal wireless connection, data processor passes through wireless transmission module and transmits the testing result to mobile terminal. Through setting up wireless transmission module, realized the remote transmission of testing result, widened the use scene of check out test set, intelligent degree is higher.
In a second aspect, the present invention provides a method for using the nucleic acid detecting device of the first aspect for non-disease diagnosis and/or treatment, the method comprising:
the sample to be detected is added into the reagent tube and mixed with the extraction reagent in the reagent tube to carry out nucleic acid extraction reaction to obtain a reactant, the detection needle tube punctures the reagent tube, the reactant in the reagent tube flows into the liquid inlet cabin through the needle tube and is shunted to each amplification cabin from the liquid inlet cabin, the reactant is mixed with the amplification reagent in the amplification cabin to complete nucleic acid amplification to obtain a reaction liquid, the reaction liquid is subjected to color change or no color change under the action of a color developing agent, and light beams emitted by the detection light source irradiate the reaction liquid in the amplification cabin.
The invention adopts visible light detection, a color-developing agent is required to be added into a reaction system, after nucleic acid amplification is finished, the color change of a reaction product is/is not generated due to the existence of the color-developing agent, a detection light source can be an LED lamp or an incandescent lamp, light beams irradiate to reaction liquid, color light reflected by the reaction liquid is received by a color sensor and the color change is identified, so that the detection result is judged. Optional color developers include phenol red and bromothymol blue.
As a preferable technical solution of the present invention, the using method further comprises:
the detection light source respectively irradiates the reaction liquid before and after amplification, the reaction liquid reflects different/same color light before and after amplification, the color light before and after amplification is respectively received by the color sensor and transmitted to the data processor, and the data processor judges a detection result according to the color change of the reaction liquid before and after amplification and controls the indicator lamp array to display the detection result.
In a third aspect, the present invention provides a use of the nucleic acid detecting apparatus of the first aspect for the real-time detection of nucleic acid samples of various types and scenes.
Compared with the prior art, the invention has the beneficial effects that:
the nucleic acid detection device provided by the invention adopts a visible light detection mode, and a complex light path system required by fluorescence detection is omitted, so that the device can be miniaturized and lightened, the operation process is simplified, the operation difficulty and the detection threshold are reduced, and the device is particularly suitable for the real-time detection of nucleic acid samples in public occasions such as customs, families, schools and the like.
Drawings
FIG. 1 is an exploded view of a nucleic acid detecting apparatus according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a reagent tube according to an embodiment of the present invention.
Wherein, 1-baffle; 2-a needle tube; 3-a seal; 4-upper cover plate; 5-sealing the film; 6-a reflector; 7-amplification plate; 8-a light-transmitting plate; 9-heating the film; 10-a color sensor; 11-a detection light source; 12-an upper circuit board; 13-a lower circuit board; 14-reagent tube.
Detailed Description
It is to be understood that in the description of the present invention, the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be taken as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
It should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "disposed," "connected" and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
The technical scheme of the invention is further explained by the specific implementation mode in combination with the attached drawings.
In one embodiment, the invention provides a nucleic acid detection device capable of home self-testing, which comprises a shell, wherein a circuit board is arranged in the shell, an amplification board 7 and a detection light source 11 are arranged on the circuit board, at least one amplification cabin is arranged on the amplification board 7, one end of the shell is inserted into a needle tube 2, and one end of the needle tube 2 extending into the shell is butted with the amplification cabin; the nucleic acid detecting apparatus further includes a reagent tube 14 (shown in FIG. 2), and the reagent tube 14 is pierced by the needle tube 2, and the reagent in the reagent tube 14 flows into the amplification chamber through the reagent tube 14 to amplify the nucleic acid.
The nucleic acid detection device provided by the invention adopts a visible light detection mode, and a complex light path system required by fluorescence detection is omitted, so that the device can be miniaturized and lightened, the operation process is simplified, the operation difficulty and the detection threshold are reduced, and the device is particularly suitable for the real-time detection of nucleic acid samples in public occasions such as customs, families, schools and the like.
Further, an amplification plate 7 is disposed in the central region of the circuit board.
Further, at least one amplification chamber is arranged on the outer edge of the amplification plate 7 along the circumferential direction.
Furthermore, the center of the amplification plate 7 is also provided with a liquid inlet cabin which is independently communicated with each amplification cabin, one end of the needle tube 2 extending into the shell is communicated with the liquid inlet cabin, and reaction liquid flows into the liquid inlet cabin through the needle tube 2 and flows into each amplification cabin through the liquid inlet cabin in a shunting manner.
Further, the periphery of the amplification plate 7 is provided with a detection light source 11, and the detection light source 11 is positioned on the circuit board. Further, the detection light source 11 is a visible light source, and may be an LED lamp or an incandescent lamp, for example.
Further, the circuit board comprises an upper circuit board 12 and a lower circuit board 13 which are electrically plugged, and the amplification board 7 is located on the upper circuit board 12.
Furthermore, the periphery of the amplification plate 7 is provided with the same number of detection light sources 11 as the number of the amplification cabins, and each detection light source 11 corresponds to one amplification cabin.
Further, the upper circuit board 12 is provided with a driving motor, the driving motor is electrically connected with the amplification board 7, and the driving motor is used for driving the bottom board to intermittently rotate.
Further, the periphery of the amplification plate 7 is provided with a detection light source 11, and during the period of stopping rotation, the detection light source 11 corresponds to one amplification cabin, and is switched to the next amplification cabin after rotating once.
It should be noted that there are two different detection schemes of the present invention, specifically:
the first scheme is as follows: when the amplification plate 7 is detected in a fixed manner, a plurality of detection light sources 11 need to be arranged on the periphery of the amplification plate 7, each detection light source 11 corresponds to one amplification chamber, reactants in the reagent tubes 14 flow into different amplification chambers through liquid inlet chambers respectively, the detection light sources 11 are started to irradiate the corresponding amplification chambers, different amplification reagents are put into the different amplification chambers in advance, and different amplification reactions are carried out. Although this solution requires a plurality of detection light sources 11, it is advantageous in that different detection means can be performed on the sample to be detected at the same time.
Scheme II: when the amplification plate 7 is detected in a rotating mode, only one detection light source 11 needs to be arranged on the periphery of the amplification plate 7, the amplification plate 7 is driven to rotate by a driving motor, reactants in the reagent tube 14 respectively flow into different amplification cabins through liquid inlet cabins in an initial state, different amplification reagents are put into the different amplification cabins in advance, the detection light source 11 is aligned to one of the amplification cabins, light beams emitted by the detection light source 11 irradiate the reactants in the amplification cabins, and a detection result of a detection sample in the amplification cabins is obtained through color detection; and after the detection is finished, rotating the amplification plate 7, switching to the next amplification cabin, and repeating the steps to detect the samples to be detected in each amplification cabin one by one. The scheme saves the cost of redundant detection light sources 11, but only one detection means can be carried out on the sample to be detected at the same time.
Furthermore, the exterior of the detection light source 11 is covered with a reflector 6 with a single side opening, the opening of the reflector 6 faces the amplification chamber, and the light beam emitted by the detection light source 11 is reflected and gathered by the reflector 6 and then irradiates the corresponding amplification chamber.
Further, the reflector 6 is fixed on the circuit board; further, the fixing manner is welding fixing.
Further, a heating film 9 is arranged between the amplification plate 7 and the circuit board, and the heating film 9 is used for heating or cooling the reactant in the amplification chamber.
Further, the heating film 9 is of an annular structure.
Further, a color sensor 10 is arranged between the amplification board 7 and the circuit board, the color sensor 10 is electrically connected with the circuit board, the detection light source 11, the amplification cabin and the color sensor 10 are positioned on the same light path, and light beams emitted by the detection light source 11 penetrate through reactants in the amplification cabin and then are received by the color sensor 10.
Further, the color sensor 10 is located at a central hollow of the annular heating film 9.
Further, a light-transmitting plate 8 is further arranged between the amplification plate 7 and the circuit board, a light path channel is formed inside the light-transmitting plate 8, the detection light source 11, the amplification cabin, the light path channel and the color sensor 10 are located on the same light path, and visible light emitted by the detection light source 11 passes through the amplification cabin and is introduced into the color sensor 10 through the light path channel.
Further, the light-transmitting plate 8 is located at the central hollow-out position of the annular heating film 9.
The invention aims to provide a light-transmitting plate 8 which comprises: (1) visible light beams emitted by different amplification cabins are gathered, so that the detection precision is improved; (2) visible light emitted by different amplification cabins is distinguished, and the mutual influence of different visible light beams is avoided, so that result misjudgment is avoided.
Furthermore, an upper cover plate 4 is arranged in the shell, and the upper cover plate 4 is buckled with the amplification plate 7.
Further, a sealing film 5 is provided on the bonding surface between the upper cover plate 4 and the amplification plate 7.
The sealing film 5 of the present invention is made of an elastic material, and the elastic material is required to have characteristics of hydrophobicity, no adsorption of nucleic acid, high temperature resistance, etc., and the specific type of the elastic material is not required or limited. The sealing film 5 is provided between the upper cover plate 4 and the amplification plate 7, and when the upper cover plate 4 and the amplification plate 7 are engaged with each other, the sealing effect between the upper cover plate 4 and the amplification plate 7 is improved by the sealing film 5.
Furthermore, a liquid inlet is formed in the surface of the upper cover plate 4, one end of the needle tube 2 is inserted into the liquid inlet, and when the detection is started, the needle tube 2 penetrates through the liquid inlet chamber, breaks the sealing film 5 and then extends into the liquid inlet chamber.
Furthermore, a baffle plate 1 is arranged at one end of the needle tube 2 far away from the upper cover plate 4, and a sealing piece 3 is arranged on the periphery of the needle tube 2.
The sealing element 3 provided by the invention is of a jacket structure, elastic materials are filled in the jacket, and the sealing element 3 is mainly used for sealing the upper cover plate 4, so that the whole nucleic acid detection device can only be contacted with the external environment through the needle tube 2.
Further, the shell is provided with an indicator light array, the circuit board is integrally provided with a data processor, the data processor is respectively and independently electrically connected with the indicator light array and the color sensor 10, the color sensor 10 transmits an optical signal to the data processor, and the data processor instructs the indicator light array to display a positive or negative detection result according to the optical signal.
The invention realizes the qualitative analysis of a sample to be detected and outputs the detection result quickly and intuitively through the detection logic formed by the detection light source 11, the color sensor 10, the data processor and the indicator light array, the detection light source 11 emits visible light and irradiates on a reactant, an optical signal emitted by the reactant after irradiation is received by the color sensor 10 and then transmitted to the data processor, the detection result is obtained in the data processor through the optical signal, and the indicator light array is controlled to display positive/negative according to the detection result.
It should be noted that the present invention can simultaneously perform different amplification reactions (different amplification reagents are placed in the amplification chamber in advance) on a sample to be tested, thereby detecting positive/negative results of different diseases. Each sample to be detected corresponds to one indicator light or one group of indicator lights, and the data processor controls the corresponding indicator lights to change according to the detection results of different samples, the color change mode of the indicator lights is not specifically required and specially limited, in principle, a detector can determine the detection result through the change of the indicator lights, and exemplarily, the following indication modes can be adopted to display the detection result:
the first scheme is as follows: the detection result is indicated by means of the on/off or color change of a single lamp, for example: when the indicator lamp is turned on, the corresponding sample to be detected is positive, and when the indicator lamp is kept off, the corresponding sample to be detected is negative; of course, the detection result can also be indicated in a color change mode.
Scheme II: the two indicating lamps are a group of indicating detection results, one indicating lamp is a positive indicating lamp, the other indicating lamp is a negative indicating lamp, if the positive indicating lamp is lighted, the sample to be detected is positive, and if the negative indicating lamp is lighted, the sample to be detected is negative.
Further, the present invention may also adopt other result display modes, such as: and the remote display of the result is realized through a wireless transmission technology. The specific scheme can be selected as follows: set up wireless transmission module in the casing, data processor and wireless transmission module electric connection, wireless transmission module and mobile terminal wireless connection, data processor passes through wireless transmission module and transmits the testing result to mobile terminal. Through setting up wireless transmission module, realized the remote transmission of testing result, widened the use scene of check out test set, intelligent degree is higher.
In another embodiment, the present invention provides a method for using the nucleic acid detecting device provided in the above embodiment for non-disease diagnosis and/or treatment, the method comprising:
(1) adding a sample to be detected into a reagent tube 14, mixing the sample with an extraction reagent in the reagent tube 14 to perform a nucleic acid extraction reaction to obtain a reactant, puncturing the reagent tube 14 by a detection needle tube 2, enabling the reactant in the reagent tube 14 to flow into a liquid inlet cabin through the needle tube 2, and shunting the reactant to each amplification cabin from the liquid inlet cabin, mixing the reactant with the amplification reagent in the amplification cabins to complete nucleic acid amplification to obtain a reaction liquid, wherein the reaction liquid is subjected to color change or not subjected to color change under the action of a color developing agent, and visible light emitted by a detection light source 11 irradiates the reaction liquid in the amplification cabins;
(2) the detection light source 11 irradiates the reaction solution before and after amplification respectively, the reaction solution reflects different/same color lights before and after amplification, the color lights before and after amplification are received by the color sensor 10 respectively and transmitted to the data processor, the data processor judges a detection result according to the color change of the reaction solution before and after amplification, and controls the indicator light array to display the detection result.
The invention adopts visible light detection, a color-developing agent is required to be added into a reaction system, after nucleic acid amplification is finished, the color change of a reaction product is/is not generated due to the existence of the color-developing agent, a visible light source can be an LED lamp or an incandescent lamp, visible light irradiates reaction liquid, color light reflected by the reaction liquid is received by a color sensor 10 and the color change is identified, so that the detection result is judged. Optional color developers include phenol red and bromothymol blue.
In another embodiment, the present invention provides a use of the nucleic acid detecting device according to the above embodiment for the instant detection of nucleic acid samples of various types and scenes. Including but not limited to customs, homes, schools, and the like.
The applicant declares that the above description is only a specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and it should be understood by those skilled in the art that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are within the scope and disclosure of the present invention.
Claims (10)
1. The nucleic acid detection device capable of realizing the household self-test is characterized by comprising a shell, wherein a circuit board is arranged in the shell, an amplification board and a detection light source are arranged on the circuit board, at least one amplification cabin is arranged on the amplification board, one end of the shell is inserted into a needle tube, and one end of the needle tube, which extends into the shell, is butted with the amplification cabin;
the nucleic acid detection device also comprises a reagent tube which is penetrated through the needle tube, and reactants in the reagent tube flow into the amplification cabin through the reagent tube to carry out nucleic acid amplification.
2. The nucleic acid detecting apparatus according to claim 1, wherein the amplification plate is disposed in a central region of the circuit board;
preferably, at least one amplification cabin is arranged on the outer edge of the amplification plate along the circumferential direction;
preferably, the center of the amplification plate is further provided with a liquid inlet cabin, the liquid inlet cabins are independently communicated with the amplification cabins, one end of the needle tube extending into the shell is communicated with the liquid inlet cabins, and reaction liquid flows into the liquid inlet cabins through the needle tube and is shunted by the liquid inlet cabins to enter the amplification cabins;
preferably, the detection light source is arranged on the periphery of the amplification plate and is positioned on the circuit board;
preferably, the detection light source is a visible light source.
3. The nucleic acid detecting device according to claim 1 or 2, wherein the circuit board comprises an upper circuit board and a lower circuit board which are electrically connected, and the amplification board is located on the upper circuit board;
preferably, the periphery of the amplification plate is provided with the same number of detection light sources as the amplification cabins, and each detection light source corresponds to one amplification cabin.
4. The apparatus according to claim 3, wherein a driving motor is disposed on the upper circuit board, the driving motor is electrically connected to the amplification plate, and the driving motor is configured to drive the base plate to intermittently rotate;
preferably, the periphery of the amplification plate is provided with a detection light source, during the period of stopping rotation, the detection light source corresponds to one of the amplification cabins, and the detection light source is switched to the next amplification cabin after rotating once.
5. The nucleic acid detecting device according to any one of claims 1 to 4, wherein a reflector with a single side opening is covered and fastened outside the detecting light source, the opening of the reflector faces the amplification chamber, and light beams emitted by the detecting light source are reflected and gathered by the reflector and then irradiate the corresponding amplification chamber;
preferably, the reflector is fixed on the circuit board;
preferably, the fixing mode is welding fixing.
6. The nucleic acid detecting device according to any one of claims 1 to 5, wherein a heating film is disposed between the amplification plate and the circuit board, and the heating film is used for heating or cooling the reactant in the amplification chamber;
preferably, the heating film is of an annular structure;
preferably, a color sensor is arranged between the amplification board and the circuit board, the color sensor is electrically connected with the circuit board, the detection light source, the amplification cabin and the color sensor are positioned on the same light path, and light beams emitted by the detection light source are received by the color sensor after passing through reactants in the amplification cabin;
preferably, the color sensor is positioned at the central hollow-out part of the annular heating film;
preferably, a light-transmitting plate is further arranged between the amplification plate and the circuit board, a light path channel is formed in the light-transmitting plate, the detection light source, the amplification cabin, the light path channel and the color sensor are located on the same light path, and light beams emitted by the detection light source pass through the amplification cabin and are introduced into the color sensor through the light path channel;
preferably, the light-transmitting plate is located at a hollow-out position in the center of the annular heating film.
7. The nucleic acid detecting device according to claim 5 or 6, wherein an upper cover plate is further provided in the case, and the upper cover plate and the amplification plate are engaged with each other;
preferably, a sealing film is arranged on the joint surface of the upper cover plate and the amplification plate;
preferably, the surface of the upper cover plate is provided with a liquid inlet, one end of the needle tube is inserted into the liquid inlet, and when the detection is started, the needle tube penetrates through the liquid inlet cabin and pierces the sealing film to extend into the liquid inlet cabin;
preferably, a baffle is arranged at one end of the needle tube, which is far away from the upper cover plate, and a sealing piece is arranged on the periphery of the needle tube;
preferably, the shell is provided with an indicator light array, the circuit board is provided with a data processor in an integrated manner, the data processor is respectively and independently electrically connected with the indicator light array and the color sensor, the color sensor transmits an optical signal to the data processor, and the data processor instructs the indicator light array to display a positive or negative detection result according to the optical signal.
8. A method for using the nucleic acid detecting device according to any one of claims 1 to 7 for the purpose of non-disease diagnosis and/or treatment, the method comprising:
the sample to be detected is added into the reagent tube and mixed with the extraction reagent in the reagent tube to carry out nucleic acid extraction reaction to obtain a reactant, the detection needle tube punctures the reagent tube, the reactant in the reagent tube flows into the liquid inlet cabin through the needle tube and is shunted to each amplification cabin from the liquid inlet cabin, the reactant is mixed with the amplification reagent in the amplification cabin to complete nucleic acid amplification to obtain a reaction liquid, the reaction liquid is subjected to color change or no color change under the action of a color developing agent, and light beams emitted by the detection light source irradiate the reaction liquid in the amplification cabin.
9. The use of claim 8, wherein said use further comprises:
the detection light source respectively irradiates the reaction liquid before and after amplification, the reaction liquid reflects different/same color light before and after amplification, the color light before and after amplification is respectively received by the color sensor and transmitted to the data processor, and the data processor judges a detection result according to the color change of the reaction liquid before and after amplification and controls the indicator lamp array to display the detection result.
10. Use of the nucleic acid detecting apparatus according to any one of claims 1 to 7, wherein the nucleic acid detecting apparatus is used for real-time detection of nucleic acid samples of various types and scenes.
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| CN1458866A (en) * | 2000-07-28 | 2003-11-26 | 基因系统公司 | Device for heat-dependent chain amplication of target nucleic acid sequences |
| EP1878496A1 (en) * | 2006-07-14 | 2008-01-16 | Roche Diagnostics GmbH | Apparatus for performing nucleic acid analysis |
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| CN110161003A (en) * | 2019-05-17 | 2019-08-23 | 深圳市刚竹医疗科技有限公司 | Optical detection apparatus and real time fluorescent quantitative nucleic acid amplification detection system |
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| CN1458866A (en) * | 2000-07-28 | 2003-11-26 | 基因系统公司 | Device for heat-dependent chain amplication of target nucleic acid sequences |
| CN102353569A (en) * | 2002-05-13 | 2012-02-15 | 贝克顿·迪金森公司 | Protease inhibitor sample collection system |
| EP1878496A1 (en) * | 2006-07-14 | 2008-01-16 | Roche Diagnostics GmbH | Apparatus for performing nucleic acid analysis |
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