CN217127434U - Nucleic acid qualitative detection device capable of realizing visual display of result - Google Patents
Nucleic acid qualitative detection device capable of realizing visual display of result Download PDFInfo
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Abstract
The utility model provides a nucleic acid qualitative detection device which can realize the visual display of the result, the nucleic acid qualitative detection device comprises a shell, a reaction module, a temperature control module, a detection module, an interpretation module and a moving module are arranged in the shell; the reaction module comprises at least one placing frame, at least one detection tube is inserted into the placing frame, the detection module comprises an optical sensor and a detection light source, and the detection tube is driven by the moving module to move to a position between the optical sensor and the detection light source, so that the detection light source, the detection tube and the optical sensor are positioned on the same light path; the interpretation module comprises a data processor and an indicator light array which are electrically connected. The utility model provides a nucleic acid qualitative detection device can pass through pilot lamp visual display testing result, and convenient operation is applicable to the nucleic acid sample instant detection of various types and scene.
Description
Technical Field
The utility model belongs to the technical field of nucleic acid detection, a realize result visual display's qualitative detection device of nucleic acid is related to.
Background
The polymerase chain reaction (nucleic acid) is a molecular biology technique for amplifying specific DNA fragments, which can be regarded as specific DNA replication in vitro. The nucleic acid is prepared by using DNA which is denatured into single strand at 95 ℃ in vitro, combining a primer and the single strand at low temperature (about 60 ℃) according to the principle of base complementary pairing, adjusting the temperature to the optimal reaction temperature (about 72 ℃) of DNA polymerase, and synthesizing a complementary strand by the DNA polymerase along the direction from phosphoric acid to pentose. The nucleic acid instrument manufactured based on polymerase is actually a temperature control device, and can be well controlled among a denaturation temperature, a renaturation temperature and an extension temperature.
CN210237635U relates to a portable nucleic acid detection device, including power module, casing, operation and display module and the detection processing module that sets up in the casing for the power supply, operation and display module, detection processing module are connected electrically between. This detection device passes through the power module power supply, realizes the detection to nucleic acid reaction liquid and will detect data processing analysis through detecting processing module, then feeds back operation and display module with the analysis result, realizes this detection device's test operation and result demonstration through operation and display module, and the structure is small and exquisite, simple. Compared with the existing fluorescence imaging detection method, the detection method for the nucleic acid reaction result does not need the steps of photographing and the like, greatly simplifies the operation flow, shortens the experiment operation time and reduces the experiment cost.
CN212800301U discloses a portable nucleic acid detection device, including detection box, nucleic acid reaction prefabricated pipe, fluorescence probe and heating device, detection box upper end opening is provided with the support ring in the upper end, nucleic acid reaction prefabricated pipe fixed connection just extrudes the through-hole in support ring center on the support ring and the middle part down, fluorescence probe fixed connection just faces the nucleic acid reaction prefabricated pipe convex part down at the lower terminal surface of support ring, heating device is installed to the middle part below of nucleic acid reaction prefabricated pipe, heating device fixed connection is in the detection box. This scheme is heated to the settlement temperature through heating device, will sample the overhead sample of test paper and crowd into the prefabricated intraductal reaction that reacts of nucleic acid reaction, adopts fluorescence probe to survey, realizes nucleic acid short-term test, and the structure is simplified greatly, and portable, cost greatly reduced.
CN109536368A discloses a portable convective nucleic acid amplification detection device, which mainly comprises an amplification part and a detection part. The amplification part comprises a heater, a water storage tank, a heat conduction module and a heat dissipation module; the detection part comprises an optical module, a smart phone and a fixing module thereof. The device uses a heater to heat water to generate water vapor, and obtains the reaction temperature required by convective nucleic acid by heating the heat conducting part with the help of the water vapor, thereby realizing the nucleic acid amplification process. Meanwhile, based on the smart phone, a detection module compatible with various types of smart phones is designed, a fluorescence image of a convective nucleic acid reaction process is collected through a camera of the smart phone, and qualitative detection is completed by means of application software on the smart phone. During the detection process, the nucleic acid reaction tube C is placed into a heating device to complete the nucleic acid amplification reaction, after the amplification reaction is completed, the detection module is fixed on the smart phone, and the nucleic acid tube C is inserted into the detection module, so that the qualitative detection of the nucleic acid amplification result can be completed by using the smart phone.
The operation of the existing nucleic acid detection device is complex, and the detection result cannot be intuitively obtained, so that the design of the nucleic acid detection device capable of intuitively displaying the detection result is required.
SUMMERY OF THE UTILITY MODEL
Not enough to prior art exists, the utility model aims to provide a realize the qualitative detection device of nucleic acid of result visual display, the utility model discloses a detection module and interpretation module mutually support and have realized the qualitative analysis of the sample that awaits measuring and output the testing result fast directly perceivedly.
To achieve the purpose, the utility model adopts the following technical proposal:
in a first aspect, the utility model provides a nucleic acid qualitative detection device for realizing visual display of results, which comprises a shell, wherein a reaction module, a temperature control module, a detection module, an interpretation module and a moving module are arranged in the shell, and the temperature control module is positioned below the reaction module and heats or cools the reaction module; the moving module is positioned at one side of the reaction module;
the reaction module comprises at least one placing rack, at least one detection tube is inserted into the placing rack, nucleic acid amplification reaction is carried out in the detection tube, and the moving module drives the placing rack to move back and forth integrally along the horizontal direction;
the detection module comprises an optical sensor and a detection light source, the detection tube is driven by the moving module to move to a position between the optical sensor and the detection light source, so that the detection light source, the detection tube and the optical sensor are positioned on the same light path, a reaction system in the detection tube generates an optical signal under the irradiation of the detection light source, and the optical sensor receives the optical signal generated by the amplification reaction system;
the interpretation module comprises a data processor and an indicator light array which are electrically connected, the optical sensor transmits an optical signal to the data processor, and the data processor instructs the indicator light array to display a positive or negative detection result according to the optical signal.
The utility model discloses a detection module and interpretation module mutually support and have realized the qualitative analysis to the sample that awaits measuring and output the testing result fast directly perceivedly, and detection module mainly used sends and detects light (exciting light or visible light) and shines to the reaction liquid on, and the optical signal that the reaction liquid shines after sending transmits to the circuit board of interpreting after optical sensor receives, acquires the testing result through optical signal in the circuit board of interpreting to control the pilot lamp array according to the testing result and show masculinity/negativity. The utility model provides a nucleic acid qualitative detection device can pass through pilot lamp visual display testing result, and convenient operation is applicable to the nucleic acid sample instant detection of various types and scene.
The utility model discloses in, optical sensor and detection light source quantity are only one, when detecting the sample that awaits measuring in a certain detecting tube, need drive rack moving as a whole through motion module to remove the detecting tube to between optical sensor and the detection light source, make detection light source, detecting tube and optical sensor be located same light path, detect the completion back, motion module drives the rack again and impels one by one, checks the sample that awaits measuring in each detecting tube and detects one by one.
As an optimized technical scheme of the utility model, the shell include uncovered casing and set up in the apron of the uncovered department of casing.
An operation panel is embedded in the cover plate.
The cover plate is of a flip structure.
One side of the shell is provided with a key switch and a power socket.
As an optimized technical scheme of the utility model, reaction module still include at least one centrifuging tube rack.
As an optimized technical scheme, temperature control module including refrigeration piece and temperature sensor, the refrigeration piece be located the rack bottom, temperature sensor and data processor electric connection, data processor feedback control the refrigeration piece, temperature sensor be used for detecting the reaction temperature in the test tube to with temperature data transmission to data processor, data processor carries out the logic judgement and controls the heating or the refrigeration temperature of refrigeration piece according to the judged result to temperature data.
As an optimized technical proposal, the bottom of each placing rack is respectively provided with an independent heating and/or refrigerating piece.
It should be noted that if the types of reagents placed in the two placing racks are the same, the same refrigerating sheet can be shared; if the actual types placed in the two placing racks are different, a refrigerating sheet which can independently heat or refrigerate needs to be arranged respectively, and the optimal reaction temperatures of different reaction reagents are controlled respectively.
As an optimized technical scheme of the utility model, the interpretation module still include the interpretation circuit board, the interpretation circuit board on the integration be provided with data processor and pilot lamp array.
As an optimized technical scheme of the utility model, detection module still include detection circuit board, optical sensor and detection light source integration set up on detection circuit board.
The detection light source emits light with different central wavelengths to the detection tube.
It should be noted that the utility model provides a nucleic acid qualitative detection device can adopt fluorescence detection, also can adopt visible light to detect, and the central wavelength of the detection light source that different detection methods adopted is different. If a fluorescence detection mode is adopted, the wavelength centers of the adopted detection light sources are different according to the difference of fluorescence detection channels, if the FAM channel is detected, the detection light source with the central wavelength of 470nm is used, and if the ROX channel is detected, the detection light source with the central wavelength of 595nm is used; if the visible light detection method is adopted, the detection light source is a white light source.
As a preferred technical solution of the present invention, the indicator array includes at least one indicator.
The utility model discloses in, utilize motion module to drive the rack and remove to the sample that awaits measuring in the different detecting tubes detects one by one, every sample that awaits measuring corresponds an pilot lamp or a set of pilot lamp, and the detection circuit board controls corresponding pilot lamp according to the testing result of different samples and changes, the utility model discloses do not do concrete requirement and special restriction to the change pattern of pilot lamp, in principle, the testing result can be confirmed through the change of pilot lamp to the measurement personnel, exemplarily, can adopt following instruction mode to show the testing result:
the first scheme is as follows: the detection result is indicated by means of the on/off or color change of a single lamp, for example: when the indicator lamp is turned on, the corresponding sample to be detected is positive, and when the indicator lamp is kept off, the corresponding sample to be detected is negative; of course, the detection result can also be indicated in a color change mode.
Scheme II: the two indicating lamps are a group of indicating detection results, one indicating lamp is a positive indicating lamp, the other indicating lamp is a negative indicating lamp, if the positive indicating lamp is lighted, the sample to be detected is positive, and if the negative indicating lamp is lighted, the sample to be detected is negative.
As an optimized technical scheme of the utility model, the qualitative detection device of nucleic acid still include heat radiation module and power, heat radiation module be used for reaction module's quick heat dissipation.
And a heat insulation plate is also arranged between the temperature control module and the heat dissipation module.
The utility model discloses in, key switch, supply socket and power form the electric connection of series connection relation, and supply socket is as power input's interface and external power supply electric connection, through the mains operated in external power supply to the casing, and key switch then is used for controlling the start-up and the bring to rest of complete machine equipment.
As an optimized technical scheme, the shell in still be provided with wireless transmission module, data processor and wireless transmission module electric connection, wireless transmission module and mobile terminal wireless connection, data processor passes through wireless transmission module and transmits testing result to mobile terminal.
The utility model discloses a set up wireless transmission module, realized the remote transmission of testing result, widened check out test set's use scene, intelligent degree is higher.
The utility model provides a nucleic acid qualitative detection device can adopt fluorescence detection or visible light to detect.
When fluorescence detection is used, the specific nucleic acid detection steps include:
taking out a detection tube with a fluorescence amplification reaction system, adding a sample nucleic acid to be detected, placing the detection tube in a placing rack, and heating or cooling the detection tube by a temperature control module according to different reaction conditions to start a nucleic acid amplification reaction;
(II) when the amplification reaction is carried out, the detection light source emits excitation light with a specific wave band to irradiate on the amplification reaction system, the fluorescence signal excited by the amplification reaction system is received by the optical sensor and then is transmitted to the data processor, the data processor judges the detection result of the sample to be detected according to the fluorescence signal, and the result of the sample to be detected is displayed through the indicator lamp and is negative or positive.
When visible light detection is used, specific nucleic acid detection steps include:
(1) adding a sample to be detected, an amplification reaction reagent and a color reagent into a detection tube, placing the detection tube in a placing rack, heating or cooling the detection tube by a temperature control module according to different reaction conditions, starting a nucleic acid amplification reaction by the temperature control module and a heat dissipation module, ending after the nucleic acid amplification is finished, and cooling the temperature of an amplification reaction system to room temperature;
(2) the detection light source emits white light to irradiate the amplification reaction system, the color of the reaction liquid generates an optical signal under the irradiation of the white light, the optical sensor receives the optical signal and transmits the optical signal to the data processor, the data processor judges the detection result of the sample to be detected according to the received optical signal, and the result of the sample to be detected is displayed through the indicator light and is negative or positive.
The utility model discloses in, if adopt the visible light detection, then need add the colour development agent to the reaction system, accomplish the back at nucleic acid amplification, because the existence of colour development agent makes the reaction product take place the colour and change, the detection light source adopts the visible light source, like the LED lamp, visible light shines to the reaction product, and the colour light of reaction product reflection is received by optical sensor and discerns the colour and changes to judge the testing result. Optional color developers include phenol red and bromothymol blue.
Compared with the prior art, the beneficial effects of the utility model are that:
(1) the utility model discloses a detection module and interpretation module mutually support and have realized the qualitative analysis to the sample that awaits measuring and output the testing result fast directly perceivedly, and detection module mainly used sends detection light (exciting light or visible light) and shines to the reaction liquid on, and the optical signal that the reaction liquid shines after sending transmits to data processor after optical sensor receives, acquires the testing result through optical signal in data processor to show positive/negative according to testing result control indicator lamp array. The utility model provides a nucleic acid qualitative detection device can pass through pilot lamp visual display testing result, convenient operation for the nucleic acid sample instant detection of various types and scene.
(2) The optical sensor and the detection light source are only one in number, when a to-be-detected sample in one detection tube is detected, the placing frame needs to be driven to move integrally through the movement module, so that the detection tube is moved between the optical sensor and the detection light source, the detection tube and the optical sensor are located on the same light path, after detection is completed, the movement module drives the placing frame to advance one by one, and the to-be-detected sample in each detection tube is checked and detected one by one.
Drawings
FIG. 1 is an exploded view of a qualitative nucleic acid detecting apparatus according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a detection module and an interpretation module according to an embodiment of the present invention;
wherein, 1-cover plate; 2-a shell; 3-an operation panel; 4, placing a rack; 5-an interpretation module; 6-a detection module; 7-a temperature control module; 8-a key switch; 9-power socket; 10-a heat insulation plate; 11-a heat dissipation module; 12-a power supply; 13-an array of indicator lights; 14-interpreting the circuit board; 15-detecting a light source; 16-an optical sensor; 17-detecting the circuit board.
Detailed Description
It is to be understood that in the description of the present invention, the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for the purpose of convenience and simplicity of description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention.
It should be noted that, unless explicitly stated or limited otherwise, the terms "disposed," "connected" and "connected" in the description of the present invention are to be construed broadly, and may for example be fixedly connected, detachably connected or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements.
The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings.
In a specific embodiment, the utility model provides a realize result visual display's qualitative detection device of nucleic acid, the nucleic acid detection device include the shell, the shell includes uncovered casing 2 and sets up in 2 uncovered department's of casing apron 1, apron 1 is the flip formula structure, embedding operating panel 3 in the apron 1, casing 2 one side is provided with key switch 8 and power 12 socket 9. The outer dimensions of the housing are 101mm × 101mm × 101 mm.
Set up reaction module, temperature control module 7, detection module 6, interpretation module 5 and removal module in the shell, detection module 6 and interpretation module 5 are located reaction module both sides respectively, and temperature control module 7 is located reaction module below, carries out the nucleic acid amplification reaction in the reaction module, and removal module is located reaction module one side.
The reaction module comprises at least one placing rack 4, at least one detection tube is inserted into the placing rack 4, nucleic acid amplification reaction is carried out in the detection tube, and the moving module drives the placing rack to move back and forth along the horizontal direction. Optionally, the reaction module further comprises at least one centrifuge tube placement rack 4.
The detection module 6 comprises an optical sensor 16 and a detection light source 15, the detection tube is driven by the moving module to move to a position between the optical sensor and the detection light source, so that the detection light source, the detection tube and the optical sensor are positioned on the same light path, the amplification reaction system generates an optical signal under the irradiation of the detection light source 15, and the optical sensor 16 receives the optical signal generated by the amplification reaction system. The detection light source emits light with different central wavelengths to the detection tube, if a fluorescence detection mode is adopted, the detection light source 15 adopts different wavelength centers of the detection light source 15 according to the difference of fluorescence detection channels, if the FAM channel is detected, the detection light source 15 with the central wavelength of 470nm is used, and if the ROX channel is detected, the detection light source 15 with the central wavelength of 595nm is used; if the visible light detection method is used, the detection light source 15 is selected to be a white light source. The detection module 6 further comprises a detection circuit board 17, and the optical sensor 16 and the detection light source 15 are integrally arranged on the detection circuit board 17.
The temperature control module 7 comprises a refrigeration piece and a temperature sensor, the refrigeration piece is positioned at the bottom of the placing frame 4, and furthermore, in order to realize independent heating or refrigeration of different placing frames 4, the bottom of each placing frame 4 is respectively provided with an independent heating and/or refrigeration piece. Temperature sensor and data processor electric connection, data processor feedback control refrigeration piece, temperature sensor are used for detecting the reaction temperature in the check tube to with temperature data transmission to data processor, data processor carries out logical judgement and controls the heating or the refrigeration temperature of refrigeration piece according to the judged result to temperature data.
The interpretation module 5 comprises an interpretation circuit board 14, a data processor and an indicator light array 13, the data processor and the indicator light array 13 are electrically connected and integrally arranged on the interpretation circuit board 14, the optical sensor 16 transmits optical signals to the data processor, and the data processor instructs the indicator light array 13 to display positive or negative detection results according to the optical signals.
The utility model discloses can carry out nucleic acid testing to a plurality of detection samples simultaneously, every detection sample corresponds an pilot lamp or a set of pilot lamp, and detection circuit board 17 controls corresponding pilot lamp according to the testing result of different samples and changes, the utility model discloses do not do concrete requirement and special restriction to the change mode of pilot lamp, in principle, the testing personnel can through the change of pilot lamp confirm the testing result can, exemplarily, can adopt following instruction mode to show the testing result:
the first scheme is as follows: the detection result is indicated by means of the on/off or color change of a single lamp, for example: when the indicator lamp is turned on, the corresponding sample to be detected is positive, and when the indicator lamp is kept off, the corresponding sample to be detected is negative; of course, the detection result can also be indicated in a color change mode.
Scheme II: the two indicating lamps are a group of indicating detection results, one indicating lamp is a positive indicating lamp, the other indicating lamp is a negative indicating lamp, if the positive indicating lamp is lighted, the sample to be detected is positive, and if the negative indicating lamp is lighted, the sample to be detected is negative.
The nucleic acid qualitative detection device further comprises a heat dissipation module 11 and a power supply 12 positioned below the heat dissipation module 11, the heat dissipation module 11 is positioned below the temperature control module 7, and a heat insulation plate 10 is further arranged between the temperature control module 7 and the heat dissipation module 11. Still be provided with wireless transmission module in the shell, data processor and wireless transmission module electric connection, wireless transmission module and mobile terminal wireless connection, data processor passes through wireless transmission module and transmits the testing result to mobile terminal.
The utility model discloses a detection module 6 and interpretation module 5 mutually support and have realized the qualitative analysis to the sample that awaits measuring and output the testing result fast directly perceivedly, detection module 6 mainly used sends and detects light (exciting light or visible light) and shines to the reaction liquid on, the optical signal that the reaction liquid shines after sending transmits to interpretation circuit board 14 after optical sensor 16 receives, obtain the testing result through optical signal in interpretation circuit board 14, and show positive/negative according to testing result control indicator lamp array 13. The utility model provides a nucleic acid qualitative detection device can pass through pilot lamp visual display testing result, and convenient operation is applicable to the nucleic acid sample instant detection of various types and scene.
In another embodiment, the present invention provides a method for qualitatively detecting nucleic acid, the method comprising:
the amplification reaction system generates optical signals after being irradiated by the detection light source 15, the optical sensor 16 receives the optical signals and transmits the optical signals to the data processor, and the data processor instructs the indicator light array 13 to display positive or negative detection results according to the optical signals.
The utility model provides a nucleic acid qualitative detection device both can be applicable to fluorescence detection and can be applicable to visible light detection, carries out the detailed description to the concrete testing process of two kinds of detection methods below.
The first scheme is as follows: the nucleic acid qualitative detection is carried out by adopting fluorescence, and the specific detection steps comprise:
taking out a detection tube provided with a fluorescence amplification reaction system, adding nucleic acid of a sample to be detected, placing the detection tube in a placing frame 4, and heating or cooling the detection tube by a temperature control module 7 according to different reaction conditions to start a nucleic acid amplification reaction;
(II) when the amplification reaction is carried out, the detection light source 15 emits exciting light with different wave bands to irradiate on the amplification reaction system, the fluorescence signal excited by the amplification reaction system is received by the optical sensor 16 and then is transmitted to the data processor, the data processor judges the detection result of the sample to be detected according to the fluorescence signal, and the result of the sample to be detected is displayed through the indicator lamp and is negative or positive.
Scheme II: the method adopts visible light to carry out nucleic acid qualitative detection, and comprises the following specific detection steps:
(1) adding a sample to be detected, an amplification reaction reagent and a color reagent into a detection tube, placing the detection tube in a placing rack 4, heating or cooling the detection tube by a temperature control module 7 according to different reaction conditions, starting a nucleic acid amplification reaction by starting the temperature control module 7 and a heat dissipation module 11, ending after the nucleic acid amplification is finished, and cooling the temperature of an amplification reaction system to room temperature;
(2) the detection light source 15 emits white light to irradiate the amplification reaction system, the color of the reaction liquid generates an optical signal under the irradiation of the white light, the optical sensor 16 receives the optical signal and transmits the optical signal to the data processor, the data processor judges the detection result of the sample to be detected according to the received optical signal, and the result of the sample to be detected is displayed through the indicator lamp and is negative or positive.
Application example 1
The application example provides a specific use step of the nucleic acid qualitative detection device in the process of carrying out fluorescence detection on the novel coronavirus:
1. extracting the new crown sample by using QIAamp Viral RNA MiniKit to obtain sample nucleic acid; in addition, the extraction can also adopt nucleic acid extraction reagents (record number: Yuejiu apparatus 20150092) produced by Guangzhou and Shikui biotechnology limited to extract the new crown sample;
2. preparing a reaction system:
2.1, adding Tris-HCl buffer solution, (NH) 4 ) 2 SO 4 KCl, magnesium chloride and the like are mixed to prepare 5 × RT PCR reaction buffer solution;
2.2, synthesizing a pair of specific primers and a TaqMan probe P1 according to the N gene sequence of the new corona virus nucleic acid, synthesizing a pair of specific primers and a TaqMan probe P2 according to the E gene sequence of the new corona virus nucleic acid, and synthesizing a pair of specific primers and a TaqMan probe P3 according to the ACE2 gene sequence of the new corona virus nucleic acid to form a probe guiding mixture;
2.3, mixing hot start Taq enzyme, reverse transcriptase, dNTPs and the like into an RT enzyme system;
2.4, uniformly mixing the reaction buffer, the probe mixture and the RT enzyme system according to the proportion provided in the table 1 to obtain a reaction system:
TABLE 1
3. Sequentially adding 20 mu L of extracted positive sample nucleic acid, 20 mu L of extracted negative quality control material and 20 mu L of extracted positive quality control material into the reaction system, tightly covering the detection tube, and centrifuging at 3000-5000rpm for 15 seconds;
4. the detection device provided by the utility model is turned on, the indicator light is fully on after the system self-detection, and then the system is turned off; transferring the detection tube into the placing rack 4, lighting the corresponding indicator lamp, and closing the cover plate 1;
5. the mobile phone opens the applet and realizes wireless connection with the nucleic acid qualitative detection device, the operator selects a fluorescence channel on the applet according to the table 2, and sets amplification conditions according to the table 3:
TABLE 2
TABLE 3
6. Starting clicking, and automatically operating the system;
7. after the amplification is finished, the instrument buzzes and displays the detection results, which are shown in table 4:
TABLE 4
| Serial number | Test sample | The result of the detection | Display mode of result |
| 1 | Positive sample | Positive for | The indicator light is on |
| 2 | Positive quality control product | Positive for | The indicator light is on |
| 3 | Negative quality control product | Negative of | The indicator light is not on |
Application example 2
The application example provides a specific use step of the nucleic acid qualitative detection device in the process of detecting the novel coronavirus through visible light:
1. extracting new corona sample with QIAamp Viral RNA MiniKit or nucleic acid extraction or purification reagent (docket No. 20150092 Yueji Instrument) produced by Guangzhou and Shikui biotechnology Limited to obtain sample nucleic acid;
2. the reaction system was configured according to the concentrations of the components provided in table 5:
TABLE 5
3. Synthesizing a pair of specific primers and a TaqMan probe P1 according to a new corona virus nucleic acid N gene sequence, synthesizing a pair of specific primers and a TaqMan probe P2 according to a new corona virus nucleic acid E gene sequence, and synthesizing a pair of specific primers and a TaqMan probe P3 according to a new corona virus nucleic acid ACE2 gene sequence to form a probe mixture;
4. uniformly mixing the reaction system and the probe mixture to obtain an amplification reagent (at the moment, the amplification reagent is purple red);
5. sequentially adding 20uL of extracted positive sample nucleic acid, 20uL of extracted negative quality control material and 20uL of extracted positive quality control material into the amplification system, tightly covering the detection tube, and centrifuging at 3000-plus 5000rpm for 15 seconds;
6. the detection device provided by the utility model is turned on, the indicator light is fully on after the system self-detection, and then the system is turned off; transferring the detection tube into the placing rack 4, lighting the corresponding indicator lamp, and closing the cover plate 1;
7. the mobile phone opens the small program to realize wireless connection with the nucleic acid qualitative detection device, and an operator sets the amplification condition on the small program to react for 30min at 56 ℃;
8. starting clicking, and automatically operating the system;
9. when the operation is finished, the instrument buzzes and displays the detection result, and the detection result is shown in table 6:
TABLE 6
| Serial number | Test sample | The result of the detection | Display mode of result |
| 1 | Positive sample | Positive for | The indicator light is on |
| 2 | Positive quality control product | Positive for | The indicator light is on |
| 3 | Negative quality control product | Negative of | The indicator light is not on |
Note: if the color of the amplification reagent changes from purple red to yellow, the corresponding indicator light is on, and the result is positive; if the color of the amplification reagent is not changed, the corresponding indicator lamp is kept turned off, and the display is negative.
The applicant states that the above description is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and those skilled in the art should understand that any changes or substitutions easily conceivable by those skilled in the art within the technical scope of the present invention are within the protection scope and the disclosure scope of the present invention.
Claims (10)
1. A nucleic acid qualitative detection device for realizing visual display of a result is characterized by comprising a shell, wherein a reaction module, a temperature control module, a detection module, an interpretation module and a moving module are arranged in the shell, and the temperature control module is positioned below the reaction module and used for heating or cooling the reaction module; the moving module is positioned at one side of the reaction module;
the reaction module comprises at least one placing rack, at least one detection tube is inserted into the placing rack, nucleic acid amplification reaction is carried out in the detection tube, and the moving module drives the placing rack to move back and forth integrally along the horizontal direction;
the detection module comprises an optical sensor and a detection light source, the detection tube is driven by the moving module to move to a position between the optical sensor and the detection light source, so that the detection light source, the detection tube and the optical sensor are positioned on the same light path, a reaction system in the detection tube generates an optical signal under the irradiation of the detection light source, and the optical sensor receives the optical signal generated by the amplification reaction system;
the interpretation module comprises a data processor and an indicator light array which are electrically connected, the optical sensor transmits an optical signal to the data processor, and the data processor instructs the indicator light array to display a positive or negative detection result according to the optical signal.
2. The qualitative nucleic acid detecting device of claim 1, wherein the housing comprises an open casing and a cover plate disposed at the opening of the casing;
an operation panel is embedded in the cover plate;
the cover plate is of a flip structure;
one side of the shell is provided with a key switch and a power socket.
3. The apparatus for qualitative detection of nucleic acid according to claim 1, wherein the reaction module further comprises at least one centrifuge tube placing rack.
4. The qualitative nucleic acid detection device of claim 1, wherein the temperature control module comprises a refrigeration piece and a temperature sensor, the refrigeration piece is located at the bottom of the placement frame, the temperature sensor is electrically connected with the data processor, the data processor controls the refrigeration piece in a feedback manner, the temperature sensor is used for detecting the reaction temperature in the detection tube and transmitting the temperature data to the data processor, and the data processor performs logic judgment on the temperature data and controls the heating or refrigerating temperature of the refrigeration piece according to the judgment result.
5. The qualitative nucleic acid detecting device of claim 1, wherein a cooling plate for independent heating and/or cooling is disposed at the bottom of each rack.
6. The apparatus according to claim 1, wherein the interpretation module further comprises an interpretation circuit board, and the interpretation circuit board is integrated with a data processor and an indicator light array.
7. The qualitative nucleic acid detecting device of claim 1, wherein the detecting module further comprises a detecting circuit board, and the optical sensor and the detecting light source are integrally disposed on the detecting circuit board;
the detection light source emits light with different central wavelengths to the detection tube.
8. The qualitative nucleic acid detecting device of claim 1, wherein the array of indicator lights comprises at least one indicator light.
9. The qualitative nucleic acid detecting device according to claim 1, further comprising a heat dissipating module and a power supply, wherein the heat dissipating module is used for rapid heat dissipation of the reaction module;
and a heat insulation plate is also arranged between the temperature control module and the heat dissipation module.
10. The qualitative nucleic acid detection device of claim 1, wherein a wireless transmission module is further disposed in the housing, the data processor is electrically connected to the wireless transmission module, the wireless transmission module is wirelessly connected to the mobile terminal, and the data processor transmits the detection result to the mobile terminal through the wireless transmission module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121425250.1U CN217127434U (en) | 2021-06-25 | 2021-06-25 | Nucleic acid qualitative detection device capable of realizing visual display of result |
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