CN219117454U

CN219117454U - Portable nucleic acid isothermal amplification device

Info

Publication number: CN219117454U
Application number: CN202222995893.0U
Authority: CN
Inventors: 罗迪贤; 王丽; 刘权; 文小莎; 陈尚
Original assignee: Union Shenzhen Hospital of Huazhong University of Science and Technology
Current assignee: Union Shenzhen Hospital of Huazhong University of Science and Technology
Priority date: 2022-11-10
Filing date: 2022-11-10
Publication date: 2023-06-02
Anticipated expiration: 2032-11-10

Abstract

The utility model provides a portable nucleic acid isothermal amplification device, which comprises a shell, wherein a heating module and a control module which are electrically connected are arranged in the shell; the heating module comprises a first heat conducting plate and a second heat conducting plate, wherein a first limit groove and a second limit groove are respectively formed in the surface of one side, opposite to the first heat conducting plate and the second heat conducting plate, of the first heat conducting plate, a first accommodating cavity for accommodating a reagent tube is formed in the first limit groove and the second limit groove, accommodating grooves are formed in the surface of one side, far away from the first heat conducting plate and the second heat conducting plate, of the first heat conducting plate, a heating plate is arranged in the accommodating grooves, and the heating plate is electrically connected with the control module; the top of casing is provided with the upper cover plate, test window has been seted up to the surface of upper cover plate, and reagent pipe by test window stretches into in the first chamber that holds. The utility model is convenient to carry, can be used for home detection, and can meet the temperature requirement of isothermal amplification.

Description

Portable nucleic acid isothermal amplification device

Technical Field

The utility model belongs to the technical field of nucleic acid detection, and relates to a portable nucleic acid isothermal amplification device.

Background

Infectious diseases are a clinically important disease type with highest incidence, such as influenza virus and the like, which cause serious injury to human beings. Most of the current diagnosis of pathogenic microorganisms uses bacterial culture and drug susceptibility tests. However, the method is easy to cause cross contamination to cause false positive or false negative, and the detection time is long, generally 3-7 days, and the result cannot be timely and accurately reported.

Currently, molecular diagnostics is increasingly becoming an important method for detecting pathogenic microorganisms using a large number of methods. Among them, fluorescent quantitative PCR (polymerase chain reaction ) is a molecular biological technique for amplifying specific DNA fragments, and can be regarded as specific DNA replication in vitro, and can greatly increase a minute amount of DNA. However, the use of this technique relies on thermal cycling, requires specialized equipment, and is not suitable for field detection, home detection or rapid screening because of the cumbersome, inconvenient to move and expensive equipment.

The detection method which is studied to be mature in the prior art is isothermal amplification, namely amplification times which are even higher than that of common PCR can be achieved in a short time, thermal cycling is not needed, and the isothermal amplification is combined with a CRISPR/Cas (constant palindromic repeated sequence cluster/constant palindromic repeated sequence cluster associated protein system, clustered Regularly Interspaced Palindromic Repeats/CRISPR-associated proteins system) system, and can be determined through the change of fluorescent signals.

Disclosure of Invention

Aiming at the defects in the prior art, the utility model aims to provide a portable nucleic acid isothermal amplification device which is convenient to carry, can be used for home detection and can meet the temperature requirement of isothermal amplification.

To achieve the purpose, the utility model adopts the following technical scheme:

the utility model provides a portable nucleic acid isothermal amplification device, which comprises a shell, wherein a heating module and a control module which are electrically connected are arranged in the shell;

the heating module comprises a first heat conducting plate and a second heat conducting plate, wherein a first limit groove and a second limit groove are respectively formed in the surface of one side, opposite to the first heat conducting plate and the second heat conducting plate, of the first heat conducting plate, a first accommodating cavity for accommodating a reagent tube is formed in the first limit groove and the second limit groove, accommodating grooves are formed in the surface of one side, far away from the first heat conducting plate and the second heat conducting plate, of the first heat conducting plate, a heating plate is arranged in the accommodating grooves, and the heating plate is electrically connected with the control module;

the top of casing is provided with the upper cover plate, test window has been seted up to the surface of upper cover plate, and reagent pipe by test window stretches into in the first chamber that holds.

According to the portable nucleic acid isothermal amplification device provided by the utility model, the reagent tube is placed in the accommodating cavity formed by the first heat-conducting plate and the second heat-conducting plate for amplification, so that cross contamination can be avoided, the device is small in size and convenient to carry, the portable nucleic acid isothermal amplification device can be used for household detection, the heating plate is used for heating to the set temperature, the result can be judged, the temperature requirement of isothermal amplification can be met, and the detection speed can be increased.

The present utility model does not specifically limit or specifically require the judging method, and in order to help those skilled in the art to better understand the overall technical scheme and working process of the present utility model, the present utility model exemplarily provides the judging method:

and inserting the detection test strip into the reagent tube, staying for 1min, taking out the test strip, waiting for a plurality of minutes, observing whether the test strip has two strips, if so, judging positive, if only one strip is negative, and if not, indicating that the test strip fails.

As a preferable technical scheme of the utility model, one side of the first heat conduction plate far away from the second heat conduction plate is sequentially provided with a first heat insulation plate and a first heat dissipation plate, and one side of the second heat conduction plate far away from the first heat conduction plate is sequentially provided with a second heat insulation plate and a second heat dissipation plate.

That is, in the utility model, a first heat insulation board and a first heat dissipation board are sequentially arranged on one side of the first heat conduction board far away from the reagent tube, and a second heat insulation board and a second heat dissipation board are sequentially arranged on one side of the second heat conduction board far away from the reagent tube. According to the utility model, the heating plate converts electric energy into heat energy to realize rapid temperature rise, the first heat conduction plate and the second heat conduction plate have good heat conduction performance to realize heat conduction between the heating plate and the reagent tube, the first heat insulation plate and the second heat insulation plate are used for heat insulation, the first heat insulation plate and the second heat insulation plate are used for heat dissipation in the shell, and the use safety performance is ensured.

As a preferable technical scheme of the utility model, the first heat insulation plate is detachably connected with the second heat insulation plate, a first positioning groove and a second positioning groove are respectively formed on the surface of one side of the first heat insulation plate, which is opposite to the second heat insulation plate, and the first positioning groove and the second positioning groove form a second accommodating cavity for accommodating the first heat insulation plate and the second heat insulation plate.

In the utility model, the first heat insulating plate is connected with the second heat insulating plate, the first positioning groove and the second positioning groove form a second accommodating chamber for accommodating the first heat conducting plate and the second heat conducting plate, and meanwhile, the first limiting groove of the first heat conducting plate and the second limiting groove of the second heat conducting plate form a first accommodating chamber for accommodating the reagent tube.

It should be noted that, the detachable connection mode of the first heat insulation board and the second heat insulation board is not particularly limited or specially required, and in order to help those skilled in the art to better understand the overall technical scheme and the working process of the utility model, the utility model exemplarily provides a connection mode of realizing the first heat insulation board and the second heat insulation board by adopting the positioning pin. The method comprises the following steps: at least one fixing hole is formed in the surface of one side, opposite to the first heat insulating plate and the second heat insulating plate, of each of the first heat insulating plate and the second heat insulating plate, one end of the locating pin is inserted into the fixing hole of the first heat insulating plate, the other end of the locating pin is aligned with the fixing hole of the second heat insulating plate for connection, and in order to avoid affecting the first heat insulating plate and the second heat insulating plate, the fixing hole is formed in the side of the first locating groove or the second locating groove. It will be understood, of course, that other means of attachment that enable the first heat shield to be secured to the second heat shield are also within the scope and disclosure of the present utility model, and that other means of attachment that have been disclosed in the prior art or that have not been disclosed in the new art may be employed in the present utility model as well.

As a preferable technical scheme of the utility model, a first bulge is arranged on one side of the first heat radiation plate, which is close to the first heat insulation plate, and a second bulge is arranged on one side of the second heat radiation plate, which is close to the second heat insulation plate.

The surface of first heat insulating board and second heat insulating board has seted up first direction through-hole and second direction through-hole respectively, first arch passes first direction through-hole, the second arch passes the second direction through-hole.

As a preferable technical scheme of the utility model, one side of the first radiating plate and one side of the second radiating plate, which are far away from the first bulge and the second bulge respectively, are provided with a plurality of radiating fins so as to improve the radiating effect in the shell.

As a preferable technical scheme of the utility model, an adjusting module is further arranged in the shell, the adjusting module comprises a supporting base, the supporting base is fixed on the bottom surface of the shell, and a sliding base is further movably connected to the supporting base.

The support base is used for supporting the first heat insulation plate and the first heat radiation plate, and the sliding base is used for driving the second heat radiation plate and the second heat insulation plate to move so as to realize distance adjustment between the first heat insulation plate and the second heat insulation plate.

The support base is fixed on the bottom surface of the inner cavity of the shell, and the sliding base slides on the support base. After the test is finished, the first heat insulation plate and the first heat radiation plate are arranged on the supporting base to be fixed and not to move, an operator drives the second heat insulation plate and the second heat radiation plate to synchronously slide by utilizing the sliding base to detach, and the reagent tube is taken out.

As a preferable technical scheme of the utility model, a sliding groove is further formed in the surface of the supporting base, a sliding block is arranged at the bottom of the sliding base, and the sliding block slides in the sliding groove.

The support base is further provided with a boss, and the first heat dissipation plate is fixed on the boss.

As a preferable technical scheme of the utility model, the adjusting module further comprises a limiting plate, an adjusting knob and a guide rod, wherein the limiting plate is fixedly connected with the supporting base, and the limiting plate is positioned on one side, away from the second heat insulation plate, of the second heat dissipation plate.

One end of the guide rod is in transmission connection with the adjusting knob, and the other end of the guide rod penetrates through the limiting plate and abuts against the second radiating plate.

The present utility model does not specifically limit or require the structure or shape of the sliding base, the limiting plate, the adjusting knob and the guide rod, and in order to help the person skilled in the art to better understand the overall technical scheme and the working process of the present utility model, the present utility model provides the following structure by way of example:

the sliding base comprises a bottom plate and a baffle, the bottom plate and the baffle form an L-shaped structure, the second heat insulation plate and the second heat radiation plate are sequentially arranged on the bottom plate side by side, and meanwhile the same height is guaranteed with the first heat insulation plate and the first heat radiation plate on the boss of the supporting base, so that the effect of heightening is achieved, and the operation of staff is facilitated. The limiting plate is located one side of the baffle, which is far away from the second heat insulation plate, and the sliding base is prevented from sliding out of the sliding groove. The guide rod adopts a threaded rod and is meshed with the adjusting knob so as to drive the guide rod to move.

In the testing process, the reagent tube is placed in the first accommodating cavity, the sliding base is moved, the second heat-radiating plate and the second heat-insulating plate are clung to the second heat-conducting plate, then the adjusting knob is rotated, the guide rod is used for propping against the second heat-radiating plate to fix, the reagent tube is prevented from falling off, and the testing safety is ensured. After the test is finished, the adjusting knob is rotated along the reverse direction, the guide rod is loosened, the second heat radiation plate and the second heat insulation plate move a certain distance along the direction away from the reagent tube along with the sliding base, the reagent tube is taken out again, the reagent tube is prevented from being broken or damaged, and the limiting plate can prevent the sliding base from sliding out of the sliding groove.

It should be noted that the above description of the structure of each operation component does not constitute a further limitation on the protection scope of the present utility model, that is, each operation component disclosed in the prior art or not disclosed in the new technology may be used in the present utility model, and is not limited to the operation module having the above structure, and as long as the operation component having the same or similar function can be replaced at will, the technical solution obtained after replacement also falls within the protection scope and the disclosure scope of the present utility model.

As a preferable technical scheme of the utility model, a detection port is formed in the side wall surface of the first heat-conducting plate, a temperature sensor is arranged in the detection port, and the temperature sensor is electrically connected with the control module.

The temperature sensor detects the temperature of the first heat-conducting plate in real time and transmits the temperature to the control module so that an operator can adjust the temperature to achieve the purpose of constant temperature control.

As a preferable technical scheme of the utility model, the control module comprises a control main board and a main control power supply which are electrically connected, a bracket is arranged in the shell, the control main board is fixed above the bracket, and the main control power supply is positioned below the bracket and is fixed on the bottom surface of the shell.

The outer wall of casing still is provided with switch and input plug, switch and input plug electric connection the master control power, still be provided with the display screen on the upper cover plate, display screen electric connection the control mainboard.

In order to further improve the detection precision, a person skilled in the art can set a detection module in the shell according to the condition of the reagent, and perform detection by adopting fluorescence quantification. To help those skilled in the art to better understand the overall technical solution and working procedure of the present utility model, the present utility model exemplarily provides the following specific structures related to the detection module:

the detection module comprises a laser, the laser is arranged in the shell and is positioned on one side of the heating module, in the use process, the input plug is electrified, the laser excites the light to irradiate the reagent tube, the display screen reads out the fluorescent value, and whether an amplification product is generated or not can be judged according to the fluorescent value.

It should be noted that the description of the structure of the detection module above does not constitute a further limitation on the protection scope of the present utility model, that is, the detection module disclosed in the prior art or not disclosed in the new technology may be used in the present utility model, and is not limited to the operation module with the above structure, and the detection module with the same or similar function may be replaced at will, so long as the technical solution obtained after replacement falls within the protection scope and the disclosure scope of the present utility model.

Compared with the prior art, the utility model has the beneficial effects that:

according to the portable nucleic acid isothermal amplification device provided by the utility model, the reagent tube is placed in the accommodating cavity formed by the first heat-conducting plate and the second heat-conducting plate for amplification, so that cross contamination can be avoided, the device is small in size and convenient to carry, the device can be used for household detection, the heating plate is used for heating to the set temperature, the result can be judged, the temperature requirement of isothermal amplification can be met, and the detection speed is increased.

Drawings

FIG. 1 is a schematic diagram showing a portable isothermal nucleic acid amplification device according to an embodiment of the present utility model;

FIG. 2 is a schematic diagram showing the internal structure of a portable isothermal nucleic acid amplification device according to one embodiment of the present utility model;

FIG. 3 is a schematic view of a heating module according to an embodiment of the present utility model;

FIG. 4 is an exploded view of a heating module according to one embodiment of the present utility model;

FIG. 5 is a schematic structural view of a first heat-conducting plate according to an embodiment of the present utility model;

FIG. 6 is a schematic structural view of a first heat shield according to an embodiment of the present utility model;

fig. 7 is a schematic structural diagram of a first heat dissipation plate according to an embodiment of the present utility model;

FIG. 8 is a side view of a heating module provided in one embodiment of the present utility model;

FIG. 9 is a schematic view of a supporting base according to an embodiment of the present utility model;

FIG. 10 is a schematic structural view of a limiting plate according to an embodiment of the present utility model;

FIG. 11 is a schematic view of a sliding base according to an embodiment of the present utility model;

FIG. 12 is a schematic structural diagram of a control module according to an embodiment of the present utility model;

fig. 13 is a schematic structural view of a bracket according to an embodiment of the present utility model.

Wherein, 1-shell; 2-an upper cover plate; 3-a test window; 4-a heating module; 5-a control module; 6-heating plate; 7-a first heat-conducting plate; 8-a second heat-conducting plate; 9-a first heat insulating plate; 10-a second heat insulating plate; 11-a first heat dissipation plate; 12-a second heat sink; 13-a support base; 14-a sliding base; 15-limiting plates; 16-an adjusting knob; 17-a guide bar; 18-a slider; 19-boss; 20-sliding grooves; 21-a first limit groove; 22-a first accommodation chamber; 23-a first guide through hole; 24-a first positioning groove; 25-locating pins; 26-a first bump; 27-heat dissipation fins; 28-a bracket; 29-a detection port; 30-a control main board; 31-a main control power supply; 32-an input plug; 33-a power switch; 34-reagent tube; 35-display screen.

Detailed Description

It is to be understood that in the description of the present utility model, the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus are not to be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may explicitly or implicitly include one or more such feature. In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.

It should be noted that, in the description of the present utility model, unless explicitly specified and limited otherwise, the terms "disposed," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art in a specific case.

The technical scheme of the utility model is further described below by the specific embodiments with reference to the accompanying drawings.

In one embodiment, the utility model provides a portable nucleic acid isothermal amplification device, as shown in fig. 1 and 2, comprising a shell 1, wherein a heating module 4 and a control module 5 which are electrically connected are arranged in the shell 1;

the heating module 4 includes a first heat-conducting plate 7 and a second heat-conducting plate 8, as shown in fig. 3, 4 and 5, a first limit groove 21 and a second limit groove are respectively formed on one side surface of the first heat-conducting plate 7 opposite to the second heat-conducting plate 8, the first limit groove 21 and the second limit groove form a first accommodating chamber 22 for accommodating a reagent tube 34, accommodating grooves are formed on one side surface of the first heat-conducting plate 7 and one side surface of the second heat-conducting plate 8 far away from each other, a heating plate 6 is arranged in the accommodating grooves, and the heating plate 6 is electrically connected with the control module 5;

the top of casing 1 is provided with upper cover plate 2, test window 3 has been seted up to upper cover plate 2's surface, reagent pipe 34 by test window 3 stretches into in the first accommodation chamber 22.

In some embodiments, as shown in fig. 4, a first heat insulation board 9 and a first heat dissipation board 11 are sequentially disposed on a side of the first heat conduction board 7 away from the second heat conduction board 8, and a second heat insulation board 10 and a second heat dissipation board 12 are sequentially disposed on a side of the second heat conduction board 8 away from the first heat conduction board 7.

That is, in the present utility model, the first heat insulation board 9 and the first heat dissipation board 11 are sequentially disposed on the side of the first heat conduction board 7 away from the reagent tube 34, and the second heat insulation board 10 and the second heat dissipation board 12 are sequentially disposed on the side of the second heat conduction board 8 away from the reagent tube 34. According to the utility model, the heating plate 6 converts electric energy into heat energy to realize rapid temperature rise, the first heat conducting plate 7 and the second heat conducting plate 8 have good heat conducting performance, heat conduction between the heating plate 6 and the reagent tube 34 is realized, the first heat insulating plate 9 and the second heat insulating plate 10 are used for heat insulation, the first heat radiating plate 11 and the second heat radiating plate 12 are used for radiating heat in the shell 1, and the use safety performance is ensured.

In some embodiments, the first heat insulating plate 9 is detachably connected to the second heat insulating plate 10, as shown in fig. 3 and 6, a first positioning groove 24 and a second positioning groove are respectively formed on a surface of one side of the first heat insulating plate 9 opposite to the second heat insulating plate 10, and the first positioning groove 24 and the second positioning groove form a second accommodating chamber for accommodating the first heat conducting plate 7 and the second heat conducting plate 8.

In the utility model, the first heat insulating plate 9 and the second heat insulating plate 10 are connected with each other, the first positioning groove 24 and the second positioning groove form a second accommodating chamber for accommodating the first heat conducting plate 7 and the second heat conducting plate 8, and meanwhile, the first limiting groove 21 of the first heat conducting plate 7 and the second limiting groove of the second heat conducting plate 8 form a first accommodating chamber 22 for accommodating the reagent tube 34.

As shown in fig. 4 and 8, the present utility model exemplarily provides a manner of connecting the first heat insulation board 9 and the second heat insulation board 10 using the positioning pins 25. The method comprises the following steps: at least one fixing hole is respectively formed in the surface of one side, opposite to the first heat insulating plate 9 and the second heat insulating plate 10, of the first heat insulating plate 9, one end of the positioning pin 25 is inserted into the fixing hole of the first heat insulating plate 9, and the other end of the positioning pin is aligned with the fixing hole of the second heat insulating plate 10 to be connected, so that the first heat insulating plate 9 and the second heat insulating plate 10 are prevented from being influenced, and the fixing hole is formed at the side of the first positioning groove 24 or the second positioning groove.

In some embodiments, as shown in fig. 7, a first protrusion 26 is disposed on a side of the first heat dissipation plate 11 adjacent to the first heat insulation plate 9, and a second protrusion is disposed on a side of the second heat dissipation plate 12 adjacent to the second heat insulation plate 10.

The surfaces of the first heat insulating plate 9 and the second heat insulating plate 10 are respectively provided with a first guide through hole 23 and a second guide through hole, the first bulge 26 penetrates through the first guide through hole 23, and the second bulge penetrates through the second guide through hole.

In some embodiments, a plurality of heat dissipation fins 27 are disposed on a side of the first heat dissipation plate 11 and the second heat dissipation plate 12 away from the first protrusion 26 and the second protrusion, respectively, so as to improve the heat dissipation effect in the housing 1.

In some embodiments, as shown in fig. 8, an adjusting module is further disposed in the housing 1, and the adjusting module includes a support base 13, where the support base 13 is fixed on the bottom surface of the housing 1, and a sliding base 14 is further movably connected to the support base 13.

The support base 13 is used for supporting the first heat insulation board 9 and the first heat dissipation board 11, and the sliding base 14 is used for driving the second heat dissipation board 12 and the second heat insulation board 10 to move so as to adjust the distance between the first heat insulation board 9 and the second heat insulation board 10.

The support base 13 of the utility model is fixed on the bottom surface of the inner cavity of the shell 1, and the sliding base 14 slides on the support base 13. After the test is finished, the first heat insulation plate 9 and the first heat dissipation plate 11 are arranged on the supporting base 13 and are fixed and do not move, an operator drives the second heat insulation plate 10 and the second heat dissipation plate 12 to synchronously slide by utilizing the sliding base 14 for disassembly, and the reagent tube 34 is taken out.

In some embodiments, as shown in fig. 4 and 9, a sliding groove 20 is further formed on the surface of the support base 13, a sliding block 18 is disposed at the bottom of the sliding base 14, and the sliding block 18 slides in the sliding groove 20. The support base 13 is further provided with a boss 19, and the first heat dissipation plate 11 is fixed on the boss 19.

In some embodiments, as shown in fig. 4, 8 and 10, the adjusting module further includes a limiting plate 15, an adjusting knob 16 and a guiding rod 17, the limiting plate 15 is fixedly connected to the supporting base 13, and the limiting plate 15 is located on a side of the second heat dissipating plate 12 away from the second heat dissipating plate 10. One end of the guide rod 17 is in transmission connection with the adjusting knob 16, and the other end of the guide rod 17 penetrates through the limiting plate 15 and abuts against the second heat dissipation plate 12.

The present utility model does not specifically limit or require the structures or shapes of the slide base 14, the limiting plate 15, the adjusting knob 16 and the guide bar 17, and exemplarily provides the following structures:

as shown in fig. 11, the sliding base 14 includes a bottom plate and a baffle, the bottom plate and the baffle form an "L", the second heat insulation board 10 and the second heat dissipation board 12 are sequentially arranged on the bottom plate side by side, and meanwhile, the same height is guaranteed with the first heat insulation board 9 and the first heat dissipation board 11 on the boss 19 of the supporting base 13, so as to play a role in raising, and facilitate the operation of staff. The limiting plate 15 is located at one side of the baffle plate away from the second heat insulation plate 10, and prevents the sliding base 14 from sliding out of the sliding groove 20. The guide rod 17 adopts a threaded rod, and is meshed with the adjusting knob 16 to drive the guide rod 17 to move.

In the testing process, the reagent tube 34 is placed in the first accommodating chamber 22, the sliding base 14 is moved, the second heat radiation plate 12 and the second heat insulation plate 10 are clung to the second heat conduction plate 8, then the adjusting knob 16 is rotated, the guide rod 17 is used for propping against the second heat radiation plate 12 to fix, the reagent tube 34 is prevented from falling off, and the testing safety is ensured. After the test is finished, the adjusting knob 16 is rotated in the opposite direction, the guide rod 17 is loosened, the second heat dissipation plate 12 and the second heat insulation plate 10 move for a certain distance along with the sliding base 14 in the direction away from the reagent tube 34, the reagent tube 34 is taken out again, the reagent tube 34 is prevented from being broken or damaged, and the limiting plate 15 can prevent the sliding base 14 from sliding out of the sliding groove 20.

In some embodiments, as shown in fig. 5, a detection port 29 is formed on a side wall surface of the first heat conducting plate 7, and a temperature sensor is disposed in the detection port 29 and is electrically connected to the control module 5. The temperature sensor detects the temperature of the first heat-conducting plate 7 in real time and transmits the temperature to the control module 5 so that an operator can adjust the temperature to achieve the purpose of constant temperature control.

In some embodiments, as shown in fig. 2, 12 and 13, the control module 5 includes a control main board 30 and a main control power source 31 electrically connected, a bracket 28 is disposed in the housing 1, the control main board 30 is fixed above the bracket 28, and the main control power source 31 is located below the bracket 28 and is fixed on the bottom surface of the housing 1.

The outer wall of the shell 1 is further provided with a power switch 33 and an input plug 32, the power switch 33 is electrically connected with the input plug 32 and the main control power supply 31, the upper cover plate 2 is further provided with a display screen 35, and the display screen 35 is electrically connected with the control main board 30. The input plug 32 in the present utility model is a small input plug with a filter.

The present utility model does not make any specific limitation or special requirement on the judgment method, and exemplarily provides the judgment method:

the test strip is inserted into the reagent tube 34, stays for 1min, then is taken out, and after waiting for a few minutes, whether the test strip has two strips or not is observed, if two strips appear, the test strip is judged to be positive, if only one strip is negative, and if no strip exists, the test strip is indicated to be invalid.

In another embodiment, the portable nucleic acid isothermal amplification device further comprises a detection module. The detection module comprises a laser, the laser is arranged in the shell 1 and is positioned on one side of the heating module 4, in the use process, the input plug 32 is electrified, the laser excites the light to irradiate the reagent tube 34, the display screen 35 reads out the fluorescent value, and whether an amplification product is generated or not can be judged according to the fluorescent value.

According to the portable nucleic acid isothermal amplification device provided by the utility model, the reagent tube 34 is placed in the accommodating cavity formed by the first heat-conducting plate 7 and the second heat-conducting plate 8 for amplification, so that cross contamination can be avoided, the device is small in size and convenient to carry, the portable nucleic acid isothermal amplification device can be used for household detection, the heating plate 6 is used for heating to the set temperature, the result can be judged, the temperature requirement of isothermal amplification can be met, and the detection speed is increased.

The applicant declares that the above is only a specific embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and it should be apparent to those skilled in the art that any changes or substitutions that are easily conceivable within the technical scope of the present utility model disclosed by the present utility model fall within the scope of the present utility model and the disclosure.

Claims

1. The portable nucleic acid isothermal amplification device is characterized by comprising a shell, wherein a heating module and a control module which are electrically connected are arranged in the shell;

2. The portable nucleic acid isothermal amplification device according to claim 1, wherein a first heat insulation plate and a first heat dissipation plate are sequentially arranged on one side, away from the second heat conduction plate, of the first heat conduction plate, and a second heat insulation plate and a second heat dissipation plate are sequentially arranged on one side, away from the first heat conduction plate, of the second heat conduction plate.

3. The portable nucleic acid isothermal amplification device according to claim 2, wherein the first heat insulating plate is detachably connected to the second heat insulating plate, a first positioning groove and a second positioning groove are formed in a surface of one side of the first heat insulating plate opposite to the second heat insulating plate, and the first positioning groove and the second positioning groove form a second accommodating chamber for accommodating the first heat insulating plate and the second heat insulating plate.

4. The portable nucleic acid isothermal amplification device according to claim 2, wherein a first protrusion is arranged on one side of the first heat dissipation plate, which is close to the first heat insulation plate, and a second protrusion is arranged on one side of the second heat dissipation plate, which is close to the second heat insulation plate;

5. The portable nucleic acid isothermal amplification device according to claim 4, wherein a plurality of heat dissipation fins are arranged on one side of the first heat dissipation plate and the second heat dissipation plate, which are away from the first protrusion and the second protrusion, respectively.

6. The portable nucleic acid isothermal amplification device according to claim 2, wherein an adjusting module is further arranged in the housing, the adjusting module comprises a supporting base, the supporting base is fixed on the bottom surface of the housing, and a sliding base is further movably connected to the supporting base;

7. The portable nucleic acid isothermal amplification device according to claim 6, wherein a sliding groove is further formed in the surface of the support base, a sliding block is arranged at the bottom of the sliding base, and the sliding block slides in the sliding groove;

8. The portable nucleic acid isothermal amplification device according to claim 6, wherein the adjustment module further comprises a limiting plate, an adjustment knob and a guide rod, wherein the limiting plate is fixedly connected with the support base, and the limiting plate is positioned on one side of the second heat dissipation plate away from the second heat dissipation plate;

9. The portable nucleic acid isothermal amplification device according to claim 1, wherein a detection port is formed in a side wall surface of the first heat conducting plate, a temperature sensor is arranged in the detection port, and the temperature sensor is electrically connected with the control module.

10. The portable nucleic acid isothermal amplification device according to claim 1, wherein the control module comprises a control main board and a main control power supply which are electrically connected, a bracket is arranged in the shell, the control main board is fixed above the bracket, and the main control power supply is positioned below the bracket and is fixed on the bottom surface of the shell;