CN208829682U - Integrated DNA analysis instrument - Google Patents

Abstract

The utility model discloses a kind of integrated DNA analysis instrument, including sample processing system and capillary electrophoresis system；Sample processing system includes sample extraction area, PCR reaction zone and pipettor, and pipettor can move between sample extraction area, PCR reaction zone, to complete the PCR reaction after the DNA of sample is extracted and extracted, is provided with electrophoresis modules in PCR reaction zone；Capillary electrophoresis system includes capillary module, the anode tap of capillary module is connected with encapsulating component, optical sensing module is provided at detection window, cathode terminal is provided with motion platform component, and the electrophoresis modules in PCR reaction zone can be connected by motion platform component with the cathode terminal of capillary module.Each step that the utility model tests and analyzes DNA is all integrated on a compact apparatus, it is easy to operate, detection is quick, under the premise of guaranteeing accuracy, greatly improve the efficiency of DNA detection, meanwhile Integral type small-sized equipment also greatly improves mobile portability, expand can application places range.

Description

Integrated DNA analysis instrument

Technical field

The utility model relates to DNA analysis detection field more particularly to a kind of integrated DNA analysis instrument.

Background technique

DNA analysis technology has wide application field.In basic research field, DNA analysis is mainly used in genome Sequencing, gene expression spectrum analysis, gene mutation and polymorphism analysis etc.；In clinical medicine domain, clinical disease can be carried out Diagnosis, medicament research and development；In judicial expertise field, individual identification and paternity test can be carried out；In agriculture field, animals and plants can be carried out Crossbreeding research, Safety of GM Food detection.

Wherein, by taking medical law fields as an example, with the development of science and technology, forensic dna detection technique has become in one line of public security Body identification, indispensable scientific sharp weapon of fighting crime.In decades, forensic dna detection technique experienced DNA Fingerprinting Map analysis technology, amplified fragment length polymorphism technology, the big technological revolution of mitochondrial DNA detection technique three.It has sent out at present It opens up based on fluorescent marker limited loci STR composite amplification detection technique, mitochondrial DNA detection technique and snp analysis technology The technical system led.

The realization of the above technology be unable to do without corresponding detection platform, and the development of forensic dna detecting instrument determines respectively The practical performance and development speed of kind forensic dna detection technique.Therefore, which is increasingly becoming forensic science field and analysis The research hotspot of instrument field.But the defect of existing equipment mostly various degrees such as requires ratio to the professional of operation It is higher, it is unsuitable for layman's use, is difficult to be applied on a large scale in conventional forensic dna detection device in a short time And popularization.

Utility model content

Have that function is integrated, simple operation, using flexible, detection the purpose of this utility model is to provide one kind The integrated DNA analysis instrument of high-speeding and Mobile portable.

To achieve the above object, the integrated DNA analysis instrument of the utility model the specific technical proposal is:

A kind of integration DNA analysis instrument, wherein including analyzer shell, analyzer enclosure interior is provided with sample process System and capillary electrophoresis system；Sample processing system includes sample extraction area, PCR reaction zone and pipettor, and pipettor can be It moves between sample extraction area, PCR reaction zone, is extracted and with completing the DNA of sample in sample extraction area in the completion of PCR reaction zone The PCR of DNA after extraction reacts, and is provided with electrophoresis modules in PCR reaction zone；Capillary electrophoresis system includes capillary module, The cathode terminal of capillary module is provided with motion platform component, motion platform component can by PCR reaction zone electrophoresis modules with The cathode terminal of capillary module is connected, and the anode tap of capillary module is connected with encapsulating component, the detection window of capillary module Place is provided with optical sensing module.

The integrated DNA analysis instrument of the utility model has the advantage that

1) each step that DNA is tested and analyzed all is integrated on a compact apparatus, easy to operate, and detection is quick, is being protected Under the premise of demonstrate,proving accuracy, the efficiency of DNA detection is greatly improved, meanwhile, Integral type small-sized equipment also greatlys improve Mobile portability, expand can application places range.

2) from sample to as a result, user is not necessarily to operate in the PCR Lab of standard, DNA extraction process, PCR mistake Journey, PCR product mixing and Capillary Electrophoresis process, signal detection process etc. can all automate progress, and it is short to be also equipped with reagent Phase saves function, can be widely applied to the fields such as public security, the administration of justice, clinic.

Detailed description of the invention

Fig. 1 is the front view of the integrated DNA analysis instrument of the utility model；

Fig. 2 is the rear view of the integrated DNA analysis instrument of the utility model；

Fig. 3 is the structural schematic diagram of the sample processing system in Fig. 1；

Fig. 4 is the top view of the processing unit in Fig. 3；

Fig. 5 is the perspective view of the PCR reaction zone in Fig. 4；

Fig. 6 is the broken away view of the PCR reaction component in Fig. 5；

Fig. 7 is the assembling stereogram of the metal reaction slot and temperature conditioning unit in Fig. 6；

Fig. 8 is the perspective view of the displacement component in Fig. 5；

Fig. 9 is the perspective view of the liquid-transfering device in Fig. 3；

Figure 10 is the partial enlarged view of the liquid-transfering device in Fig. 9；

Figure 11 is the perspective view of the pipettor in Fig. 9；

Figure 12 is the perspective view of another embodiment of the pipettor in Fig. 9；

Figure 13 is the perspective view of the magnetic pipettor in Fig. 9；

Figure 14 is the local broken away view of the magnetic pipettor in Figure 13；

Figure 15 is the perspective view of the magnetic assembly in Figure 13；

Figure 16 is the working state figure one of the magnetic pipettor in Figure 13；

Figure 17 is the working state figure two of the magnetic pipettor in Figure 13；

Figure 18 is the structural schematic diagram of the capillary electrophoresis system in Fig. 1；

Figure 19 is the broken away view of the capillary module in Figure 18；

Figure 20 is the perspective view of the motion platform component in Figure 18；

Figure 21 is the perspective view of the encapsulating component in Figure 18；

Figure 22 is the perspective view of another embodiment of the encapsulating component in Figure 18；

Figure 23 is the broken away view of the encapsulating component in Figure 22；

Figure 24 is the front view of the another embodiment of the encapsulating component in Figure 18；

Figure 25 is the rear view of the encapsulating component in Figure 24；

Figure 26 is the front view of the main part of the encapsulating component in Figure 24；

Figure 27 is the rear view of the main part of the encapsulating component in Figure 24；

Figure 28 is the perspective view of the support component in Figure 26；

Figure 29 is the perspective view of the storage element in Figure 26；

Figure 30 is the front view of the blob of viscose element in Figure 26；

Figure 31 is the rear view of the blob of viscose element in Figure 26；

Figure 32 is the longitudinal sectional view of the blob of viscose element in Figure 26；

Figure 33 is the transverse sectional view of the blob of viscose element in Figure 26；

Figure 34 is the front view of the optical sensing module in Figure 18；

Figure 35 is the rear view of the optical sensing module in Figure 18；

Figure 36 is the index path of the optical sensing module in Figure 18.

Specific embodiment

In order to be better understood by the purpose of this utility model, structure and function, with reference to the accompanying drawing, to the utility model It is a kind of integration DNA analysis instrument do further detailed description.

Compared to traditional DNA analysis equipment, the integrated DNA analysis instrument of the utility model is integrated with sample extraction, PCR Almost all step during the DNA analyses such as reaction, electrophoretic separation, fluorescence detection makes the analytic process of DNA can be whole at one It is completed in body equipment, greatly improves the usage scenario of equipment, can be widely applied to the fields such as public security, the administration of justice, clinic.

As depicted in figs. 1 and 2, according to functional regional division, the integrated DNA analysis instrument of the utility model includes at sample Reason system 1 and capillary electrophoresis system 2, sample processing system 1 and capillary electrophoresis system 2 are arranged in analyzer shell (in figure Do not show) inside, constitute one miniaturization DNA analysis instrument.Wherein, sample processing system 1 is used to carry out DNA to sample to mention PCR reaction after taking and extracting；Capillary Electrophoresis and detection and analysis of the capillary electrophoresis system 2 for realizing reactant.Separately Outside, it is noted that be also provided with control system, power-supply system etc. accordingly in the integrated DNA analysis instrument of the utility model Complementary structure, to reach the automation of equipment operation.

Sample processing system 1

It is a preferred embodiment of the sample processing system in the utility model as shown in Fig. 3 to Figure 17.The embodiment In, sample processing system 1 includes processing unit 100 and liquid-transfering device 200, wherein and processing unit 100 includes mounting base 110, Sample extraction area 120 and PCR reaction zone 130 are provided on mounting base 110, sample extraction area 120 can carry out sample DNA is extracted, and PCR reaction zone 130 can carry out PCR reaction to the DNA after extraction；Liquid-transfering device 200 include braced frame 210, The top of processing unit 100 is arranged in mobile device 220 and pipettor 230, braced frame 210, and the setting of mobile device 220 exists In braced frame 210, pipettor 230 can be driven by mobile device 220 and be moved in braced frame 210, to realize that sample mentions It takes, the transfer of the substances in processing unit such as the sample in PCR reaction process, reagent.The sample process of the utility model as a result, System is used cooperatively by processing unit and liquid-transfering device, and the institutional operation of sample process may be implemented.

It further, is a preferred embodiment of the processing unit in the utility model as shown in Fig. 4 to Fig. 8.The embodiment In, processing unit 100 includes mounting base 110, wherein mounting base 110 is integral component, is arranged on mounting base 110 There is partition 150, the side of partition 150 is sample extraction area 120, and the other side is PCR reaction zone 130, passes through intermediate setting as a result, Partition 150 can make sample extraction area 120 and PCR reaction zone 130 relatively independent, reduce the generation mutually polluted.

Further, sample extraction area 120 is used to carry out DNA extraction to sample, including reagent storage assembly 121, sample mention Take component 122, pipette tips storage assembly for storing 123 and waste storage assembly for storing 124.Wherein, reagent storage assembly 121 can be stored at room temperature more Kind of reagent, such as magnetic bead, lysate, in conjunction with liquid, cleaning solution, rinsing liquid, eluent, isopropanol, dehydrated alcohol, Proteinase K, answer It is noted that situation according to different needs, contained amount of reagent, type be will be different in reagent storage assembly.

Further, sample extraction component 122 includes one or more reacting holes, to meet different throughput requirements, reacting hole Lower section is provided with temperature control module, and temperature control module can be heated or be cooled down to reaction is extracted.Wherein, in sample extraction component Following manner realization can be used in DNA extraction: 1) being used cooperatively with the magnetic pipettor that will be discussed later, extracted using paramagnetic particle method DNA；2) it is used cooperatively with the common pipettor that will be discussed later, and is correspondingly arranged the magnetic devices such as removable bar magnet, utilized Paramagnetic particle method extracts DNA.It should be noted that excessive limitation is not done in the utility model for specific extracting mode, as long as The extraction of DNA can be readily achieved using the device of the utility model, aforesaid way is only preferred embodiment.

Further, pipette tips storage assembly for storing 123 is used to place the pipette tips for the plurality of specifications that may be used during sample extraction； Waste storage assembly for storing 124 is for storing the pipette tips after use.When carrying out sample extraction operation, pipettor can be directly from pipette tips Target pipette tips are taken in storage assembly for storing, also directly pipette tips can be disposal in waste storage assembly for storing after the completion of liquid relief, operation side Just, the manipulation time is saved, the degree of automation of the sample processing system of the utility model is improved.

It should be noted that according to actual needs, it can also be by the sample extraction component 122 and reagent in sample extraction area 120 Storage assembly 121 merges, that is, reaction reagent is directly deposited in reacting hole, when extracting reaction, and direct handle Sample is added in the reacting hole containing reagent, and the mistake from reagent storage assembly to sample extraction component reagent adding is omitted Journey.

Further, as shown in Figure 4 and Figure 5, PCR reaction zone 130 is used to carry out PCR reaction to the DNA after extraction, including PCR reaction component 131, cryogenic agent storage assembly 132, pipette tips storage assembly for storing 133 and waste storage assembly for storing 134.Wherein, rifle Head storage assembly for storing 133 and waste storage assembly for storing 134 are similar with structure, the function in sample extraction area 120 described above, It repeats no more.

Further, PCR reaction component 131 includes reactive tank unit and temperature conditioning unit, wherein reactive tank unit is that PCR is anti- The support container answered, temperature conditioning unit are connected with reactive tank unit, for controlling to the PCR reaction temperature in reactive tank unit System.Specifically, as Fig. 6 shows, reactive tank unit includes metal reaction slot 136 and is movably arranged on 136 top of metal reaction slot Temperature control react lid 137, wherein metal reaction slot 136 is preferably made of the good metal of thermal conductivity such as gold, silver, aluminium, metal Multiple reaction tube spliced eyes are formed on reactive tank 136, PCR reaction tube can be plugged into reaction tube spliced eye, to complete PCR Reaction.

Further, as shown in fig. 6, the top of metal reaction slot 136 is arranged in temperature control reaction lid 137, wherein metal reaction The side of slot 136 is provided with motor and rotation axis, the rotation of motor powered rotatable axis, and temperature control reaction lid 137 is connected with rotation axis, Under the drive of the motor, temperature control reaction lid 137 can be opened and closed relative to metal reaction slot 136, when carrying out PCR reaction The PCR reaction tube being inserted on sealing metal reactive tank 136 guarantees going on smoothly for PCR reaction.In addition, temperature control reaction lid Heating component is additionally provided in 137, in PCR reaction, metal reaction slot 136, can be to PCR reaction tube by the effect of temperature conditioning unit In reaction solution heated, in order to avoid reaction solution evaporate into temperature control reaction lid 137 in, can start simultaneously temperature control reaction lid Heating component in 137, to guarantee being normally carried out for PCR reaction.

Further, as shown in Figure 6 and Figure 7, temperature conditioning unit includes TEC (Thermo Electric Cooler) temperature control group The top of TEC temperature control component 138 is arranged in part 138, metal reaction slot 136, and TEC temperature control component 138 can be to metal reaction slot 136 Temperature be adjusted, with meet PCR reaction temperature requirement.Preferably, due to the rate of temperature fall of TEC temperature control component 138 It is unable to satisfy the cooling requirement of PCR reaction, the lower section of TEC temperature control component 138 is additionally provided with radiator 139, wherein radiator 139 bottom is formed with heat transfer slot, and in heat transfer slot, the other end is connected with radiator fan 141 for one end setting of heat transfer sheet 140. As a result, when needing to radiate to metal reaction slot 136, start radiator fan 141, the heat transmission gas that radiator fan 141 is blown out Stream can be reached by heat transfer sheet 140 with carrying out auxiliary temperature-reducing to TEC temperature control component 138 by heat transfer sheet 140, radiator 139 The cooling requirement of PCR reaction.It is emitted in addition, radiator fan 141 can also orient aerosol issuable in device, example The aerosol processing unit being such as discharged into outside device is medium, in order to provide good reaction environment.

Further, examination needed for cryogenic agent storage assembly 132 is used to store PCR reaction or the reaction of subsequent Capillary Electrophoresis Agent, such as PCR enzyme solution, PCR primer, pcr template, PCR buffer, dNTP, formamide.Wherein, the low temperature examination in the present embodiment The structure similar with PCR reaction component 131 can be selected in agent storage assembly 132, that is, may also comprise reserve tank unit and temperature control list Member, the temperature control reaction lid 137 for distinguishing the reactive tank unit being in PCR reaction component 131 has heating function, and low temperature tries The sealing thermal insulation lid of reserve tank unit in agent storage assembly 132 only has heat insulation function, this is because cryogenic agent storage group Part 132 only need to carry out cryo-conservation to PCR reaction reagent or Capillary Electrophoresis reaction reagent, without heat etc. Reason.

Further, electrophoresis modules 135 are additionally provided in PCR reaction zone 130, so as to subsequent capillary electrophoresis system phase It is used cooperatively.Wherein, electrophoresis modules 135 are arranged on mounting base 110, are mainly used for storing Capillary Electrophoresis use in the process The substances such as Cathode buffer, water, sample, such as may include sample tube, cleaning pipe and buffer pipe, sample can be added in sample tube This, the analyte as Capillary Electrophoresis；Pure water can be added in cleaning pipe, the cleaning for capillary；Buffer liquid pipe Nei Kejia Enter buffer, for carrying out electrophoresis reaction.It should be noted that each component in the reaction unit of the utility model is in mounting base On position arrangement and it is revocable, in attached drawing be only preferred embodiment, can be adjusted flexibly according to different requirements, as PCR is anti- Answer component, location swap of reagent storage assembly etc..

Further, in order to easily complete sample extraction and PCR reaction, the reaction unit of the utility model be can be set to It moves, is matched with the movement with the pipettor in liquid-transfering device in X-direction in the Y-axis direction.Wherein, as shown in figure 8, The mounting base 110 of reaction unit may be provided on displacement component, and displacement component includes y-axis motor 161, Y-axis sliding rail 162, Y-axis Sliding block 163, Y-axis bracket 164, Y-axis sliding rail 162 are fixed on pedestal or the pedestal of other forms, and Y-axis sliding block 163 slides It is arranged on Y-axis sliding rail 162, Y-axis sliding block 163 is fixedly connected with the motor shaft of y-axis motor 161, and motor shaft can drive Y-axis sliding block 163 move back and forth along Y-axis sliding rail 162, and Y-axis bracket 164 is fixed on Y-axis sliding block 163, the mounting base of reaction unit 110 are fixed on Y-axis bracket 164.As a result, when needing drive response device to move in the Y-axis direction, y-axis motor 161 Motor shaft drive Y-axis sliding block 163 moved along Y-axis sliding rail 162, Y-axis sliding block 163 by Y-axis bracket 164 drive mounting base 110 and each component above it is mobile.

In addition, according to actual needs, the reaction unit of the utility model may be arranged as in Y-axis and Z axis direction all It is removable, to match with the pipettor 230 in liquid-transfering device 200 in the movement of X-direction, three-dimensional movement is realized, about Z axis The moving structure in direction can refer to the moving structure of above-mentioned Y direction to design or using the mobile knot of Z axis commonly used in the art Structure be not described in detail in the utility model.

It further, is a preferred embodiment of the liquid-transfering device in the utility model as shown in Fig. 9 to Figure 17.The implementation In example, liquid-transfering device 200 includes braced frame 210, mobile device 220 and pipettor 230.Wherein, braced frame 210 is gantry Rack and panel construction, including two vertically arranged support columns, and the crossbeam being horizontally installed between two support columns.Wherein, it answers It is noted that the concrete shape and size of braced frame 210 can be according to the different arrangement forms of mounting base 110 come flexibly Design, it is not limited to as shown in the figure.

Further, as shown in Figure 10, mobile device 220 includes mobile motor 221, connector 222, sliding block 223 and sliding rail 224, wherein mobile motor 221 is fixed in braced frame 210, and in the present embodiment, mobile motor 221 is specifically to be arranged The junction of support column and crossbeam in braced frame 210, the length extending direction of motor shaft and the length extending direction of crossbeam It is parallel to each other；Connector 222 is fixedly connected with motor shaft, can be moved back and forth under the drive of motor shaft；The side of sliding block 223 is solid Fixed to be arranged on connector 222, the other side is slidably connected with sliding rail 224, and sliding rail 224 is fixed in braced frame 210 On crossbeam, pipettor 230 is fixed on sliding block 223.As a result, when needing to move pipettor 230, starting movement Motor 221, mobile by motor shaft band follower link 222, connector 222 is while mobile with movable slider 223 along sliding rail 224 It is mobile, and then realize the movement to the pipettor 230 on sliding block 223.

Further, in order to improve liquid relief efficiency, there are two pipettors 230 for setting in the present embodiment, as shown in figure 9, corresponding In setting on two pipettors 230, braced frame 210, there are two mobile devices 220, two mobile devices 220 to be separately positioned on The both ends of braced frame 210, that is, being separately positioned on the junction of two support columns and crossbeam.Two pipettors 230 are set respectively It sets in two mobile devices 220, according to actual needs, optionally manipulates one of them or manipulate two pipettors simultaneously 230, efficiently to complete pipetting.

Further, in order to control the moving range of pipettor 230 in moving process, it is also provided with limit switch 240, In, as shown in Figure 10, the first limit assembly in limit switch 240 is arranged at the position of mobile motor 221, the second limit group Part is arranged on connector 222, when direction of the motor shaft with follower link 222 towards close mobile motor 221 is mobile, first Limit assembly and the second limit assembly can also move closer to, and contact with each other when pipettor 230 reaches the limit of position, realization pair The control of 230 displacement stroke of pipettor avoids causing to damage to pipettor 230 in moving process.

It further, as shown in figure 11, is a preferred embodiment of the pipettor in the utility model.In the embodiment, move Liquid device 300 includes bottom plate 310, and be arranged on bottom plate 310 injecting motor 320, syringe 330 and unload pipette tips component.Its In, it is the basic part of pipettor 300 that bottom plate 310, which is plate structure, and each component of pipettor 300 is arranged on bottom plate 310, bottom Plate 310 is fixed in the mobile device of above-mentioned liquid-transfering device, and then the reciprocating movement of pipettor is realized by mobile device.

Further, syringe 330 and injecting motor 320 are respectively fixedly disposed on bottom plate 310, the push rod of syringe 330 It is connected with motor shaft, pipette tips are connected with syringe 330, it is preferred that syringe 330 includes syringe body, syringe body One end be provided with the first connector, the first connector is connected with the motor shaft of injecting motor 320, the other end of syringe body It is provided with the second connector, the second connector is connected with pipette tips 350.As a result, when needing to draw target liq, starting injection electricity Machine 320, motor shaft drive the push rod uplink of syringe 330, suction are provided for pipette tips 350, to draw target liq；When needing to arrange Out when target liq in pipette tips 350, start injecting motor 320, motor shaft drives the push rod downlink of syringe 330, to rifle Target liq in first 350 applies thrust, and target liq is released from pipette tips 350.

Further, unloading pipette tips component includes support rod 341, and first baffle 342 and second are fixedly installed on bottom plate 310 Baffle 343, support rod 341 are arranged between first baffle 342 and second baffle 343, one end of support rod 341 and first baffle 342 are fixedly connected, and the other end is fixedly connected with second baffle 343, wherein the length extending direction and syringe of support rod 341 330 length extending direction is parallel to each other, and first baffle 342 is arranged close to injecting motor 320, and second baffle 343 is close to pipette tips 350 settings, it is preferred that support rod 341 is two, is separately positioned on the two sides of syringe 330, to guarantee the steady of equipment operation It is qualitative.

Further, the side on support rod 341 close to second baffle 343 is arranged with pipette tips press box, and pipette tips press box is frame Formula structure, including the first pressing plate 344 and the second pressing plate 345, are provided with connecting rod between the first pressing plate 344 and the second pressing plate 345 346.Wherein, the first pressing plate 344 is set on support rod 341, between first baffle 342 and second baffle 343, connection Bar 346 is arranged across second baffle 343, and the second pressing plate 345 is between second baffle 343 and pipette tips 350, that is, pipette tips pressure Frame is activity setting relative to support rod 341, and can be moved up and down along support rod 341.

Further, transmission pressing plate 347 is provided on motor shaft, transmission pressing plate 347 is connected with the push rod of syringe 330, electricity Arbor can drive the push rod uplink and downlink of syringe 330 by transmission pressing plate 347.Wherein, transmission pressing plate 347 is set in support It on bar 341, can be moved back and forth relative to support rod 341, transmission casing 348 is also arranged on support rod 341, is driven casing 348 It, can when being driven pressing plate 347 along 341 downlink of support rod between the first pressing plate 344 on transmission pressing plate 347 and pipette tips press box It pushes transmission casing 348 along 341 downlink of support rod, to push the first pressing plate 344 along 341 downlink of support rod, and then makes pipette tips pressure Frame entirety downlink, to achieve the purpose that unload pipette tips 350 from syringe 330.

Further, limit switch 360 is also provided on bottom plate 310, wherein as shown in figure 11, in limit switch 360 First limit assembly is arranged on bottom plate 310 at position corresponding with the extreme position that can unload pipette tips 350, the second limit Component setting is on transmission pressing plate 347, when motor shaft drives transmission pressing plate 347 along 341 downlink of support rod, the first limit assembly It can move closer to the second limit assembly, and contact with each other when the extreme position of pipette tips 350 can be unloaded by reaching, realize to biography The control of the displacement stroke of dynamic pressure plate 347 avoids causing to damage to each component of pipettor 300 in moving process.

Further, reset spring 349 is provided between the first pressing plate 344 and second baffle 343, it is preferred that reset bullet Spring 349 is set in the connecting rod 346 between the first pressing plate 344 and the second pressing plate 345, one end of reset spring 349 and first Pressing plate 344 is inconsistent, and the other end and second baffle 343 are inconsistent, and reset spring 349 is supported on the first pressing plate 344 and second gear Between plate 343, to maintain the relative positional relationship of pipette tips press box and support rod 341, second baffle 343.As a result, when transmission pressing plate When 347 promotion transmission casings 348 are along 341 downlink of support rod, transmission casing 348 pushes the first pressing plate 344 towards close to second gear The direction movement of plate 343 namely pipette tips press box entirety downlink, reset spring 349 is compressed at this time；When transmission pressing plate 347 is along branch 341 uplink of strut and with transmission casing 348 be detached from when, reset spring 349 is restored to reset condition, at this time 349 meeting of reset spring The first pressing plate 344 is pushed to be directed away from the direction movement namely pipette tips press box entirety uplink of second baffle 343.

The concrete operating principle of the pipettor of the present embodiment are as follows: 1) install pipette tips, under the drive of mobile device, pipettor It is moved at pipette tips storage assembly for storing, selection target pipette tips simultaneously are completed to install；2) target liq (reagent or sample etc.) is drawn, starting Injecting motor, motor shaft drive the push rod uplink of syringe by transmission pressing plate, and target liq will be inhaled into and syringe phase In pipette tips even；3) target liq is released, injecting motor is started, motor shaft is driven by transmission pressing plate under the push rod of syringe Row, target liq will be pushed out from pipette tips；4) pipette tips are unloaded, under the drive of mobile device, pipettor is moved to discarded At object storage assembly for storing, motor shaft continues to press on transmission pressing plate and is driven pressing plate along support rod downlink and is in contact with transmission casing, and pushes away Dynamic transmission casing is driven casing and pushes first pressing plate of the pipette tips press box in support rod downlink, pipette tips press box along support rod downlink Reset spring between second baffle is compressed, and the second pressing plate in pipette tips press box is in contact with pipette tips 4, and finally by pipette tips It is separated with syringe, pipette tips drop into waste storage assembly for storing；5) device resets, and motor shaft drives transmission pressing plate along support rod Uplink, transmission pressing plate are separated with transmission casing, and the reset spring between the first pressing plate and second baffle restores to the original state, to push rifle Head press box is along support rod uplink.

It further, as shown in figure 12, is another preferred embodiment of the pipettor of the utility model.In the embodiment, move Liquid device 400 includes accommodating shell 410, first motor 420, syringe 430 and unloads pipette tips component.Wherein, the setting of syringe 430 exists It accommodates inside shell 410；The outside of accommodating shell 410 is arranged in first motor 420, and motor shaft is extend into accommodating shell 410 Portion is simultaneously connected with the push rod of syringe body, to control the uplink and downlink of the push rod of syringe 430；Pipette tips 450 are located at accommodating The outside of shell 410 can be connected by pipette tips connector with syringe 430.

Further, unloading pipette tips component includes the second motor 441 and pipette tips pressing plate 442, and the setting of the second motor 441 is accommodating The outside of accommodating shell 410 is arranged in the inside of shell 410, pipette tips pressing plate 442, and motor shaft stretches out putting part body 410 and and rifle Head pressing plate 442 is connected, and for pipette tips pressing plate 442 between pipette tips 450 and pipette tips connector, motor shaft can push pipette tips pressing plate 442 It is mobile towards the direction of close pipette tips 450, and finally separate pipette tips 450 with syringe 430.

The concrete operating principle of the pipettor of the present embodiment are as follows: 1) install pipette tips, under the drive of mobile device, pipettor It is moved at pipette tips storage assembly for storing, selection target pipette tips simultaneously are completed to install；2) target liq is drawn, first motor, motor are started Axis drives the push rod uplink of syringe, and target liq will be inhaled into the pipette tips being connected with syringe；3) target liq is released, Start first motor, motor shaft drives the push rod downlink of syringe, and target liq will be pushed out from pipette tips；4) rifle is unloaded Head, under the drive of mobile device, pipettor is moved at waste storage assembly for storing, starts the second motor, and motor shaft pushes rifle Towards close to the movement of the direction of pipette tips, pipette tips pressing plate is in contact head pressing plate with pipette tips, and finally separates pipette tips with syringe, rifle Head drops into waste storage assembly for storing；5) device resets, and starts the second motor, and motor shaft drives pipette tips pressing plate to be directed away from rifle The direction of head is mobile.

It further, is the preferred embodiment of the magnetic pipettor in the utility model as shown in Figure 13 to Figure 17.The implementation In example, magnetic pipettor 500 is that magnetic assembly 520, specifically, magnetic assembly are added on the basis of above-mentioned common pipettor 520 are arranged on the accommodating shell 510 of pipettor at position corresponding with pipette tips 550, including magnetic motor 521, connecting rod 522 and magnet 523, magnetic assembly 520 can be used cooperatively with the pipette tips 550 on pipettor, to complete the extraction operation of DNA.

Further, it is provided with fixing piece 524 on the accommodating shell 510 of pipettor, motor installation is provided on fixing piece 524 Hole and motor shaft accommodating hole are provided with motor connecting hole on magnetic motor 521, are pacified the motor on fixing piece 524 by screw Dress hole is connected with the motor connecting hole on magnetic motor 521, so that magnetic motor 521 is fixed on fixing piece 524, motor Axis passes through the motor shaft accommodating hole setting on fixing piece 524, and can rotate in motor shaft accommodating hole.

Further, one end of connecting rod 522 is provided with motor shaft connecting hole, and the other end is provided with magnet accommodation groove, motor Axis is fixedly connected after passing through the motor shaft accommodating hole on fixing piece 524 with the motor shaft connecting hole in connecting rod 522, and motor shaft can Connecting rod 522 is driven to rotate, magnet 523 is fixed in magnet accommodation groove, can rotate together with connecting rod 522, with close Or far from the pipette tips 550 on pipettor 400.

As a result, as shown in Figure 16 and Figure 17, the working principle of the magnetic pipettor of the present embodiment is (to be with blood sample Example): 1) blood sample is added to cracking fluid apertures, uses the suction repeatedly of magnetic pipettor；2) heating cracks fluid apertures for a period of time Afterwards, cell can be cleaved liquid destruction, release DNA；3) using the solution in magnetic pipettor sucking cracking fluid apertures, magnetic is drained into Pearl mixes fluid apertures, uses the suction repeatedly of magnetic pipettor；4) after standing a period of time, DNA can be specifically bound with magnetic bead；5) make With the solution in magnetic pipettor sucking magnetic bead mixing fluid apertures, solution is located in the pipette tips on liquid relief component；6) starting magnetic electricity Machine drives connecting rod rotation, so that pipette tips of the magnet on liquid relief component in connecting rod, and adsorb the magnetic in pipette tips Pearl；7) solution in the pipette tips on liquid relief component is drained into magnetic bead and mixes fluid apertures, the magnetic bead containing DNA is attracted in magnetic at this time Near magnet on component；8) magnetic pipettor is moved to washing fluid apertures, starts magnetic motor, drive connecting rod rotation, with Make the magnet in connecting rod far from the pipette tips on liquid relief component, releases the absorption to the magnetic bead in pipette tips；9) magnetic liquid relief is used Device suction repeatedly, for washing magnetic bead；10) start magnetic motor, connecting rod rotation is driven, so that the magnet in connecting rod is close Pipette tips on liquid relief component adsorb the magnetic bead in pipette tips again；11) solution in the pipette tips on liquid relief component is drained into washing Fluid apertures, the magnetic bead containing DNA attracts again near the magnet in magnetic assembly at this time；12) magnetic pipettor is moved to and is washed De- fluid apertures starts magnetic motor, drives connecting rod rotation, so that the magnet in connecting rod is far from the pipette tips on liquid relief component, then Absorption of the secondary releasing to the magnetic bead in pipette tips；13) using magnetic pipettor repeatedly suction elution fluid apertures in solution, make containing The magnetic bead of DNA enters in elution fluid apertures；14) elution fluid apertures is heated, separates magnetic bead with DNA；15) magnetic is used Solution in pipettor sucking elution fluid apertures, starts magnetic motor, connecting rod rotation is driven, so that the magnet in connecting rod is close Pipette tips on liquid relief component adsorb the magnetic bead in pipette tips again；16) solution in the pipette tips on liquid relief component is drained into DNA Sample aperture obtains DNA solution.

Capillary electrophoresis system

It is a preferred embodiment of the capillary electrophoresis system in the utility model as shown in Figure 18 to Figure 36.The implementation In example, capillary electrophoresis system 2 includes mounting bracket, is provided with capillary module 600 in mounting bracket, wherein mounting bracket It is provided with motion platform component 700 at the upper position corresponding to the cathode terminal of capillary module 600, motion platform component 700 can The electrophoresis modules 135 in above-mentioned PCR reaction zone 130 are made to be connected with the cathode terminal of capillary module 600, as needed by cathode Buffer, water or sample are in contact with capillary cathode end；Position in mounting bracket corresponding to the anode tap of capillary module 600 The place of setting is provided with encapsulating component 800, and encapsulating component 800 is connected with the anode tap of capillary module 600, can be as needed by anode Buffer is in contact with capillary anode tap, and gel is injected in capillary；Corresponding to capillary module 600 in mounting bracket Optical sensing module 900, the detection window of optical sensing module 900 and capillary module 600 are provided at the position of detection window Mouth is connected, for realizing the detection and analysis of Capillary Electrophoresis.

Further, as shown in figure 19, capillary module 600 includes protective element, temperature control component and capillary 630, The inside of protective element is arranged in temperature control component and capillary 630, and temperature control component and capillary 630 are attached to one It rises.Wherein, capillary 630 can use commercial product, the elasticity produced such as Polymicro Technologies (PT) company Vitreous silica capillary is usually used artificial vitreous silica semi-finished product and draws, it is similar also can be used day heat quartz, Pyrex, a variety of plastics or molten silicon materials synthesis substance manufacture, this depends primarily on actual application demand.

Further, carrier of the capillary 630 as electrophoretic separation, is preferably arranged in a curve way the inside of protective element, is formed Smooth radian reaches the optimum efficiency of electrophoretic separation, it is of course also possible to which it is anti-to use other modes to be seated in capillary 630 The inside of protection element, such as the capillary being of a straight line type directly is arranged in the inside of protective element.In addition, in the present embodiment, It is provided only with a capillary inside one capillary module, but according to actual needs, such as in order to improve analysis throughput or analysis Efficiency etc., also can using more capillaries to be in parallel arranged to form to the form of bundle of capillary tubes, such as by 4,8,16,24 or 32 equal number of capillaries are arranged in capillary module in an array manner, do not do specific limit in the utility model to this It is fixed.

Further, protective element mainly plays the role of protecting capillary 630, including first shell 611 and second shell Body 612, first shell 611 and the interconnection of second shell 612 can form a closed chamber, and capillary 630 is directly set It is placed on the closed cavity chamber interior, protective element and capillary 630 form an entirety as a result, can not only play guarantor in this way The effect that shield capillary avoids damage to, but also can effectively improve the substitute mode of capillary.Preferably, first shell 611 It is hinged with one end of second shell 612, the other end can be opened, and can be sealed using the progress of lock form, with convenient Maintenance and replacement capillary.

Further, temperature control component includes sequentially connected heating sheet 621, thermally conductive sheet 622 and insulating trip 623, this reality It applies in example, as shown in figure 19, heating sheet 621, thermally conductive sheet 622 and insulating trip 623 are successively set on capillary 630 and second shell Between body 612, heating sheet 621 is close to second shell 612, and insulating trip 623 is close to capillary 630, wherein heating sheet 621 can Guarantee the temperature of capillary 630 in electrophoresis, it is preferred that temperature sensor is provided at heating sheet 621, with Real-time Feedback The temperature of heating sheet 621；Thermally conductive sheet 622 can make the temperature of heating sheet 621 uniformly be sufficiently conducted to entire capillary 630；Insulating trip 623 can conduct heat, and play insulation effect in high-voltage power supply, prevent the production of the electric discharge phenomena such as electric arc It is raw.Moreover it is preferred that temperature sensor is also equipped at capillary 630, with the temperature of Real-time Feedback capillary 630.

Alternatively, the mode that hot air circulation can also be used in temperature control component heats, specifically, capillary is assemblied in In insulation shell, insulation shell has air inlet and air outlet, passes through the heating sheet and fan generation hot wind in heating furnace, hot wind Entered in insulation shell internal chamber by air inlet, the capillary inside insulation shell is heated, is then arranged by air outlet Out, heating furnace is reentered, it is preferred that can be by the temperature of detection heating sheet, air inlet and air outlet come feedback regulation Temperature.

Further, as shown in figure 20, motion platform component 700, which is arranged in mounting bracket, corresponds to capillary module 600 Cathode terminal position at, including driving motor 710, sliding block 720, sliding rail 730 and carrying platform 740.Wherein, driving motor 710 are fixed in mounting bracket, and the motor shaft of driving motor 710 is connected with sliding block 720, and sliding block 720 is slidably arranged in cunning On rail 730, sliding rail 730 is fixed in mounting bracket, and sliding block 720 is connected with carrying platform 740, in PCR reaction zone 130 Electrophoresis modules 135 may be provided on carrying platform 740, and Cathode buffer can be added in electrophoresis modules 135 as needed (when electrophoresis Conducting medium), the water medium of scrap rubber (when encapsulating discharge) or sample etc..

Driving motor 710 can drive sliding block 720 to move up along sliding rail 730 as a result, with holding on movable slider 720 Carrying platform 740 and then makes the electrophoresis group on capillary cathode end and carrying platform 740 close to the cathode terminal of capillary module 600 Reagent in part 135 is in contact；Driving motor 710 can drive sliding block 720 to move down along sliding rail 730, with band movable slider 720 On cathode terminal of the carrying platform 740 far from capillary module 600, and then make electrophoresis modules 135 and the hair on carrying platform 740 The cathode terminal of tubule component 600 separates, to be convenient for changing electrophoresis modules 135.

In addition, can also increase the devices such as motor and sliding rail according to actual needs, on motion platform component becomes two dimension or three Maintenance and operation moving platform, to increase the degree of automation.For example, can not have to replacement Reagent Tube manually after becoming two-dimension moving platform, reduce Operation；And after becoming three-dimensional movement platform, multiple Reagent Tubes or 8 townhouses or 96 orifice plates etc. can be arranged, to realize continuous loading Purpose.

Further, as shown in figure 21, the sun for corresponding to capillary module 600 in mounting bracket is arranged in encapsulating component 800 At extreme position, including encapsulating motor 801, sliding block 802, sliding rail 803, syringe 804, blob of viscose element 805 and anode buffer Liquid bottle 806.Wherein, encapsulating motor 801 is fixed in mounting bracket, motor shaft and 802 phase of sliding block of encapsulating motor 801 Even, sliding block 802 is slidably arranged on sliding rail 803, and sliding rail 803 is fixed in mounting bracket, sliding block 802 and syringe 804 On push rod be connected, syringe 804 for temporarily store gel and realize capillary encapsulating.

Further, blob of viscose element 805 is fixed in mounting bracket, and three are provided in blob of viscose element 805 and is connected each other Logical first passage, second channel and third channel, wherein first passage is connected with syringe 804, second channel and sun Pole buffer bottle 806 is connected, and third channel is connected with capillary 630, and second channel and anode buffer liquid bottle 806 Between be provided with opening/shutting valve 807, to control the connection or disconnection of second channel Yu anode buffer liquid bottle 806, anode buffer liquid Conducting medium of the bottle 806 for temporarily storing anode buffer liquid, when anode buffer liquid is electrophoresis.

As a result, when needing encapsulating, close between second channel and anode buffer liquid bottle 806 in blob of viscose element 805 Opening/shutting valve 807 is in an off state second channel with anode buffer liquid bottle 806, and encapsulating motor 801 drives 802 edge of sliding block Sliding rail 803 moves down, and to push the push rod downlink in syringe 804 by sliding block 802, and then makes solidifying in syringe 804 Glue enters the first passage in blob of viscose element 805, and injects capillary 630 by third channel, completes encapsulating operation.

It further, as shown in Figure 22 and Figure 23, is another embodiment of the encapsulating component 800 in the utility model.With The difference of a upper embodiment is, in the embodiment, the outside of syringe 804 is provided with cooling module 810, with can for a long time Save gel.Specifically, cooling module 810 includes the first fixing piece 811 and the second fixing piece 812,811 He of the first fixing piece Second fixing piece 812 is connected, and to form closed chamber, syringe 804 is located in the closed chamber, the outside packet of syringe 804 It covers and is equipped with heat-conducting piece 813, heat-conducting piece 813 is connected with TEC cooling piece 814, and the outside cladding of heat-conducting piece 813 is provided with heat preservation member 815, the temperature in syringe 804 can be adjusted by TEC cooling piece 814, heat-conducting piece 813 as a result, heat preservation member 815 can To reduce heat dissipation, improves temperature and adjust efficiency.

In addition, in order to increase rate of heat dispation, it is preferred that correspond to TEC system in heat preservation member 815 and the first fixing piece 811 Be formed with opening at cold 814 position, TEC cooling piece 814 can be connected by the opening with cooling fin 816, cooling fin 816 and Radiator fan 817 is connected, and can carry out auxiliary drop to TEC cooling piece 814 from there through radiator fan 817 and cooling fin 816 Temperature further submits temperature to adjust efficiency.

It further, is the another embodiment of the encapsulating component in the utility model as shown in Figure 24 to Figure 33.With it is above-mentioned Embodiment is compared, and in the present embodiment, storage glue mode, blob of viscose structure, encapsulating mode etc. is all optimized, more to collect At change and automation.

Specifically, in the present embodiment, encapsulating component includes support component 820, storage element 830 and blob of viscose element 840, Wherein, support component 820 is basic component, for providing frame support for entire encapsulating component；The setting of storage element 830 is being propped up It supports on element 820, including gel storage chamber 831 and buffer storage chamber 832, storage has capillary electric in gel storage chamber 831 Gel needed for swimming, anode buffer liquid needed for storage has Capillary Electrophoresis in buffer storage chamber 832；In blob of viscose element 840 Be provided with a plurality of interface channel, a plurality of interface channel respectively with buffer storage chamber 832, gel storage chamber 831, capillary, filling It infuses driving element 850 to be connected, to realize the gel perfusion in capillary 630 by blob of viscose element 840.

Further, as shown in figure 28, support component 820 includes column 821, and sliding is provided with plummer on column 821 822, storage element 830 is arranged on plummer 822, wherein plummer 822 includes the first supporting part 823 and the second supporting part 824, the first supporting part 823 is used to carry the buffer storage chamber 832 in storage element 830, and the second supporting part 824 is for holding Carry the gel storage chamber 831 in storage element 830.In addition, plummer 822 is connected with driving unit 825, driving unit 825 can Plummer 822 is driven to move up and down along column 821, to facilitate the replacement to storage element 830, in the present embodiment, driving unit 825 can be used motor.

Further, as shown in figure 29, storage element 830 is an integral structure, i.e. gel storage chamber 831 and buffer storage It deposits chamber 832 and is interconnected to an entirety, as a result, when needing to replace storage element 830, motor driven support component Plummer 822 in 820 declines, and the storage element 830 that integral type is removed from plummer 822 can be completed replacement, facilitate province Thing.Certainly, according to actual needs, storage element 830 can also be made into split type structure, i.e. gel storage chamber 831 and buffering Liquid storage chamber 832 is two mutually independent structures, can increase the flexibility used in this way.

Further, as shown in Figure 26 and Figure 27, gel storage chamber 831 is connected with cooling module 860, and cooling module 860 can Temperature in gel storage chamber 831 is controlled, to guarantee that the low temperature of gel stores, in the present embodiment, cooling module 860 is set It sets on the second supporting part 824 of support component 820, it can be with system when gel storage chamber 831 is seated on the second supporting part 824 Cold element 860 is in contact.Meanwhile the outside of gel storage chamber 831 is additionally provided with warm keeping element 870, warm keeping element 870 and refrigeration Element 860 with the use of can enable gel for a long time save, avoid gel it is frequent replacement and fill, save technique at This, while only needing that the filling of gel can be realized by replacing storage element 830, reduce the complexity of operation.It should be noted that , cooling module 860 and warm keeping element 870 can use refrigeration structure and thermal insulation material commonly used in the art, this reality With particularly being limited not to this in novel.

Further, as shown in Figure 30 to Figure 33, blob of viscose element 840 is block structure, and the is provided in blob of viscose element 840 A connection channel 841, the second interface channel 842, third interface channel 843 and the 4th interface channel 844, four interface channels that This connection, it is preferred that as shown in Figure 32 and Figure 33, the first interface channel 841, the second interface channel 842, third interface channel 843 with the 4th interface channel 844 have one it is common be connected to Rendezvous Point 845, realized by the connection Rendezvous Point 845 mutual Connection.

Further, the first interface channel 841 is connected with gel storage chamber 831, the second interface channel 842 and buffer Storage chamber 832 is connected, and third interface channel 843 is connected with capillary, the 4th interface channel 844 and perfusion driving element 850 are connected.These, it is preferred to be provided with check valve (in figure between the first interface channel 841 and gel storage chamber 831 Do not show), check valve only allows gel to flow into the first interface channel 841 from gel storage chamber 831；Second interface channel 842 with it is slow Opening and closing valve 846 is provided between fliud flushing storage chamber 832, opening and closing valve 846 can control the second interface channel 842 and store up with buffer Deposit the connection and disconnection between chamber 832；Perfusion driving element 850 selects syringe commonly used in the art, as shown in figure 25, with Just power is provided for the perfusion of gel.

As a result, when needing that gel is perfused into capillary, the second interface channel 842 and buffer storage chamber 832 are closed Between opening and closing valve 846 drive syringe to provide suction so that the second interface channel 842 is disconnected with buffer storage chamber 832 Power, the gel in gel storage chamber 831 will flow into the first interface channel 841 by check valve, and then by connection Rendezvous Point 845 flow into the 4th interface channel 844 and syringe 804；Drive syringe 804 to provide thrust, 844 He of the 4th interface channel Gel in syringe 804 will flow into the second interface channel 842 and third interface channel 843 by connection Rendezvous Point 845, And then upper gel will be perfused in buffer storage chamber 832 and intercapillary channel.

It further, as shown in figure 34 to figure 36, is a preferred embodiment of optical sensing module.In the embodiment, optics Detection components 900 are arranged at the position in mounting bracket corresponding to the detection window of capillary module 600, including mounting base 901, mounting base 901 be rectangular configuration, laser 902, the first reflecting mirror 903, the second reflecting mirror 904, condenser lens 905, Capillary unit 906, objective lens 907 and third reflecting mirror 908 are arranged at the marginal position of mounting base 901, optical filter 909, sleeve lens 910 and spectrometer 911 are located at the middle position of mounting base 901.As a result, in optical sensing module 900 Excitation beam propagated between each component at the marginal position for being located at mounting base, and to the hair in capillary unit 906 Tubule is irradiated, to generate fluorescence, and the fluorescence generated then can each component for being located at the middle position of mounting base it Between propagate, enter eventually into spectrometer complete test and analyze.

Further, as shown in figs. 34 and 35, laser 902 is arranged at the one side edge of mounting base 901, preferably It is that laser 902 is extended along the first long side 912 of mounting base 901, wherein laser 902 is used to provide exciting light Beam 916, excitation beam 916 are propagated along the first long side 912 of mounting base 901, and exciting light can be tried with the fluorescence in capillary Agent is had an effect, and to generate fluorescence, realizes the optical detection of Capillary Electrophoresis.

Further, it is provided with the first reflecting mirror 903 at the position of the transmitting terminal of laser 902 on mounting base 901, Wherein, the first reflection folder is formed between the excitation beam 916 that the reflecting surface of the first reflecting mirror 903 and laser 902 issue Angle, the first reflection angle can be such that excitation beam 916 is reflected on subsequent second reflecting mirror 904 by the first reflecting mirror 903, excellent Choosing, the first reflection angle is 45 °, so that excitation beam 916 at right angles reflects at the first reflecting mirror 903.It is specific next It says, as shown in Figure 34 and Figure 36, the first corner of mounting base 901 is arranged in the first reflecting mirror 903, that is, the first long side The intersection of side 912 and the first short side 913, laser 902 is issued along the first long side 912 of mounting base 901 as a result, Excitation beam 916 can by the first reflecting mirror 903 reflection and become along mounting base 901 the first short side 913 propagate Primary event light beam 917.

Further, as shown in Figure 35 and Figure 36, the second corner of mounting base 901 is arranged in the second reflecting mirror 904, That is, the intersection of the first short side 913 and the second long side 914, the primary event light beam 917 that the first reflecting mirror 903 reflects The second reflection angle is formed between the reflecting surface of the second reflecting mirror 904, the second reflection angle can make primary event light beam 917 It being reflected on subsequent condenser lens 905 by the second reflecting mirror 904, it is preferred that the second reflection angle is also 45 °, so that Primary event light beam 917 equally at right angles reflects at the second reflecting mirror 904.

Further, as shown in Figure 35 and Figure 36, mounting base 901 is arranged in condenser lens 905 and capillary unit 906 Second long side 914, wherein condenser lens 905 can receive the between the second reflecting mirror 904 and capillary unit 906 The secondary reflection light beam 918 that two-mirror 904 reflects back, and capillary unit 906 is irradiated to after forming focus on light beam 919 In capillary on, at the optical detection window specially on capillary, to generate fluorescence.Specifically, capillary unit 906 The third corner of mounting base 901 can be located at, that is, the intersection of the second long side 914 and the second short side 915.

Further, objective lens 907 are set together with capillary unit 906, specifically, objective lens 907 and hair Optical detection window face setting on tubule, to collect the fluorescent light beam 920 issued at optical detection window.In addition, third Reflecting mirror 908 is arranged at the light outlet of objective lens 907, wherein the reflecting surface and objective lens of third reflecting mirror 908 Third reflection angle is formed between 907 fluorescent light beams 920 issued, third reflection angle can make fluorescent light beam 920 by the Three reflecting mirrors 908 are reflected on subsequent optical filter 909 and sleeve lens 910, it is preferred that and it is 45 ° that third, which reflects angle, So that fluorescent light beam 920 at right angles reflects at third reflecting mirror 908.

Further, optical filter 909, light outlet and 911 phase of spectrometer are provided at the light entrance of sleeve lens 910 Even, wherein optical filter 909 is used to will go into the impurity light beam in the triple reflection light beam 921 of sleeve lens 910 (as excited Light beam etc.) it filters out, to form detection light beam, guarantee the accuracy tested and analyzed；Sleeve lens 910 are used to will test light beam weight It is transferred in spectrometer 911 after new focusing；Spectrometer 911 is used to carry out spectrum analysis to the detection light beam after focusing.

As a result, as shown in figure 36, the course of work of the optical sensing module 900 of the utility model are as follows: 1) laser 902 Issue excitation beam 916；2) excitation beam 916 forms primary event light beam 917 by the reflection of the first reflecting mirror 903；3) one Secondary reflection light beam 917 is irradiated on the second reflecting mirror 904, forms secondary reflection light beam 918；4) secondary reflection light beam 918 irradiates Onto condenser lens 905, focus on light beam 919 is formed；5) focus on light beam 919 is irradiated on the capillary in capillary unit 906, Generate fluorescence；6) objective lens 907 collect the fluorescent light beam 920 generated in capillary；7) fluorescent light beam 920 is irradiated to third On reflecting mirror 908, triple reflection light beam 921 is formed；8) triple reflection light beam 921 is irradiated on optical filter 909, forms detection light Beam；9) detection light beam is transferred in spectrometer 911 after the focusing of sleeve lens 910 and is tested and analyzed.

It is further preferred that the setting of at least one of the first reflecting mirror 903 and third reflecting mirror 908 is adjustable On eyeglass platform, the angle of the first reflecting mirror 903 and third reflecting mirror 908 can be also adjusted by the way that eyeglass platform is adjusted, it is convenient Debugging reduces assembly precision requirement, for example, having dressed up 45.5 degree, then when the angle of the second reflecting mirror 904 is due to rigging error Its optical path can be made to enter lens by adjusting 0.5 degree of the first reflecting mirror.Wherein, this field can be used by eyeglass platform being adjusted In common angle adjustment structure, be specifically limited not to this in the utility model.

The DNA each step tested and analyzed is all integrated on a compact apparatus in the utility model, easy to operate, inspection It surveys fast, under the premise of guaranteeing accuracy, greatly improves the efficiency of DNA detection, meanwhile, Integral type small-sized equipment Greatly improve mobile portability, expand can application places range.

The utility model is further described by specific embodiment above, it should be understood that, have here The description of body, should not be construed as the restriction to the spirit and scope of the utility model, and one of ordinary skilled in the art is readding The various modifications made after reader specification to above-described embodiment belong to the range that the utility model is protected.

Claims (17)

1. a kind of integration DNA analysis instrument, which is characterized in that including analyzer shell, analyzer enclosure interior is provided with sample Processing system and capillary electrophoresis system；

Sample processing system includes sample extraction area, PCR reaction zone and pipettor, and pipettor can be reacted in sample extraction area, PCR It is moved between area, to complete in sample extraction area, the DNA of sample is extracted and the PCR of the DNA after PCR reaction zone is completed to extract is anti- It answers, is provided with electrophoresis modules in PCR reaction zone；

Capillary electrophoresis system includes capillary module, and the cathode terminal of capillary module is provided with motion platform component, and movement is flat Electrophoresis modules in PCR reaction zone can be connected by platform component with the cathode terminal of capillary module, the anode tap of capillary module with Encapsulating component is connected, and is provided with optical sensing module at the detection window of capillary module.

2. integration DNA analysis instrument according to claim 1, which is characterized in that sample processing system includes processing unit, Processing unit includes mounting base, and partition is provided on mounting base, and the side of partition is sample extraction area, other side PCR Reaction zone.

3. integration DNA analysis instrument according to claim 1 or 2, which is characterized in that PCR reaction zone includes PCR reaction group Part, PCR reaction component include reactive tank unit and temperature conditioning unit, and temperature conditioning unit includes TEC temperature control component, the setting of reactive tank unit Radiator is provided with below the top of TEC temperature control component, TEC temperature control component, the bottom of radiator is formed with heat transfer slot, passes In heat transfer slot, the other end is connected with radiator fan for one end setting of backing.

4. integration DNA analysis instrument according to claim 1 or 2, which is characterized in that the electrophoresis modules in PCR reaction zone Including sample tube, cleaning pipe and buffer pipe, it is stored with Capillary Electrophoresis process respectively in sample tube, cleaning pipe and buffer pipe Used in reagent and sample.

5. integration DNA analysis instrument according to claim 1 or 2, which is characterized in that sample processing system includes liquid relief dress It sets, liquid-transfering device includes braced frame, mobile device and pipettor, and braced frame is arranged in sample extraction area and PCR reaction zone Top, mobile device be arranged on the support frame, pipettor can by mobile device drive move on the support frame.

6. integration DNA analysis instrument according to claim 5, which is characterized in that pipettor includes syringe, syringe Push rod is connected with the motor shaft of liquid relief motor, and pipette tips are connected with syringe, and moveable pressure is provided between pipette tips and syringe Board group part, Anchor plate kit can move along towards the direction close to pipette tips, pipette tips separated with syringe.

7. integration DNA analysis instrument according to claim 6, which is characterized in that pipettor includes support rod, support rod One end is fixedly connected with first baffle, and the other end is fixedly connected with second baffle；Anchor plate kit includes the first pressing plate and the second pressure Plate is fixedly installed connecting rod between the first pressing plate and the second pressing plate, the first pressing plate is movably set on support rod, is located at first Between baffle and second baffle, connecting rod activity across second baffle be arranged, the second pressing plate between second baffle and pipette tips, Reset spring is provided between first pressing plate and second baffle；The motor shaft of motor is connected with transmission pressing plate, and transmission pressing plate is arranged On support rod, drive socket is arranged on support rod, one end of drive socket is in contact with transmission pressing plate, the other end and first Pressing plate is in contact.

8. integration DNA analysis instrument according to claim 6, which is characterized in that pipettor includes first motor and second Motor, the motor shaft of first motor are connected with the push rod of syringe, can drive push rod uplink and downlink；The motor shaft of second motor It is connected with Anchor plate kit, Anchor plate kit can be driven mobile towards the direction close to or far from pipette tips.

9. integrated DNA analysis instrument described according to claim 6 or 7 or 8, which is characterized in that be provided with magnetic on pipettor Component, magnetic assembly include magnetic motor and the magnet that is connected with magnetic motor, and magnetic motor can drive magnet rotors, with close Pipette tips or far from pipette tips, complete absorption to magnetic bead in pipette tips and desorb.

10. integration DNA analysis instrument according to claim 1, which is characterized in that capillary module include protective element, Temperature control component and capillary, temperature control component and capillary are arranged in the inside of protective element, temperature control component and Capillary is attached onto.

11. according to claim 1 or integrated DNA analysis instrument described in 10, which is characterized in that motion platform component includes driving The motor shaft of motor, sliding block, sliding rail and carrying platform, driving motor is connected with sliding block, and sliding block is slidably arranged on sliding rail, sliding block It is connected with carrying platform, the electrophoresis modules in PCR reaction zone are settable on the carrying platform.

12. according to claim 1 or integrated DNA analysis instrument described in 10, which is characterized in that encapsulating component include syringe, Blob of viscose element and anode buffer liquid bottle are provided with the first passage, second channel and third channel to communicate with each other in blob of viscose element, First passage is connected with syringe, and second channel is connected with anode buffer liquid bottle, and third channel is connected with capillary, the Be provided with opening/shutting valve between two channels and anode buffer liquid bottle, the outside cladding of syringe is provided with heat-conducting piece, heat-conducting piece with TEC cooling piece is connected, and the outside of heat-conducting piece cladding is provided with heat preservation member, is provided with fixing piece on the outside of heat preservation member, heat preservation member and Corresponding to opening is formed at the position of TEC cooling piece on fixing piece, TEC cooling piece is connected by the opening with cooling fin, is dissipated Backing is connected with radiator fan.

13. according to claim 1 or integrated DNA analysis instrument described in 10, which is characterized in that encapsulating component includes support member Part, storage element and blob of viscose element, storage element is arranged on a support element, including gel storage chamber and buffer storage chamber, Gel storage chamber is connected with cooling module, is provided with a plurality of interface channel in blob of viscose element, a plurality of interface channel respectively with buffering Liquid storage chamber, gel storage chamber, capillary, perfusion driving element are connected.

14. integration DNA analysis instrument according to claim 13, which is characterized in that support component includes column, on column Sliding is provided with plummer, and storage element is arranged on plummer, and plummer is connected with driving unit, and driving unit can drive and hold Microscope carrier is moved back and forth along column.

15. integration DNA analysis instrument according to claim 13, which is characterized in that gel storage chamber and buffer storage Chamber is an integral structure.

16. integration DNA analysis instrument according to claim 13, which is characterized in that be provided with the first company in blob of viscose element Connect road, the second interface channel, third interface channel and the 4th interface channel, the first interface channel, the second interface channel, third It is provided with a connection Rendezvous Point between interface channel and the 4th interface channel, is communicated with each other by the connection Rendezvous Point；First Interface channel is connected with gel storage chamber, and the second interface channel is connected with buffer storage chamber, third interface channel and hair Tubule is connected, and the 4th interface channel is connected with perfusion driving element, is arranged between the first interface channel and gel storage chamber There is check valve, opening and closing valve is provided between the second interface channel and buffer storage chamber.

17. according to claim 1 or integrated DNA analysis instrument described in 10, which is characterized in that optical sensing module includes installation Bottom plate has been sequentially arranged laser, the first reflecting mirror, the second reflecting mirror, condenser lens, capillary at the marginal position of mounting base Tube assembly, objective lens and third reflecting mirror, the excitation beam that laser generates is by the first reflecting mirror, the second reflecting mirror and gathers The capillary in capillary module is irradiated after focus lens, to generate fluorescence；The middle position of mounting base is provided with Optical filter, sleeve lens and spectrometer, objective lens collect the fluorescent light beam generated in capillary, and fluorescent light beam is anti-by third Enter in spectrometer after penetrating mirror, optical filter and sleeve lens, to be tested and analyzed.