WO2022160998A1 - Molecular diagnostic platform - Google Patents

Abstract

A molecular diagnostic platform, comprising: a rack; a cartridge; and a sample processing module, a sample transfer module, a thermal cycling module, and an optical module which are provided on the rack. The cartridge is configured to be used for packaging a sample to be tested and various reagents, and an operator places the cartridge on a cartridge moving assembly of the rack so as to convey the cartridge to the sample processing module; the sample processing module is configured to be used for operating the cartridge, so that the reagents in the cartridge sequentially react with the sample, and a sample solution obtained after the reaction is canned in a capillary tube on a test tube rack; and the sample transfer module is configured to be used for transferring the test tube rack to the thermal cycling module, so that the sample is subjected to a PCR reaction, and fluorescence signal acquisition is performed on the sample by means of the optical module to obtain a fluorescence imaging picture.

Description

Molecular Diagnostic Platform

CROSS-REFERENCE TO RELATED APPLICATIONS

This application requires the application number 202110126913.8 to be submitted to the State Intellectual Property Office of China on January 29, 2021, and the Chinese patent application entitled "Molecular Diagnostic Platform", and the application number to the State Intellectual Property Office of China on January 29, 2021. "202110129513.2", a Chinese patent application titled "Multi-channel Fluorescence Quantitative Detection Device and Molecular Diagnosis Platform", filed with the State Intellectual Property Office of China on January 29, 2021 with the application number of 202110129275.5, titled "Heating Device and Heating Device to Be Heated" The priority of the Chinese patent application filed with the State Intellectual Property Office of China on January 29, 2021 with the application number 202110129518.5 and the title of "Clamping Mechanism and Transfer Device", the entire contents of the above applications are approved Reference is incorporated in this application.

technical field

The present application relates to the technical field of molecular detection, and in particular, to a molecular diagnostic platform.

Background technique

With the transformation of the medical model and the continuous development of individualized medicine, the medical laboratory urgently needs fast and accurate detection methods, and molecular detection has a unique advantage. However, due to the complexity of molecular detection technology itself, "sample to result" is not only cumbersome to operate, but also takes too long to detect.

SUMMARY OF THE INVENTION

The present application provides a molecular diagnosis platform to improve the automation degree of the molecular diagnosis platform to a certain extent and shorten the detection time.

The present application provides a molecular diagnostic platform, which may include a rack, a cartridge, a sample processing module, a sample transfer module, a thermal cycling module, and an optical module; the sample processing module, the sample transfer module, and the thermal cycling module and the optical module may be respectively provided on the rack; the cartridge may be provided with a test tube rack on which capillaries may be installed; the sample processing module may be configured to operate the card cartridge, so that the sample in the cartridge completes the nucleic acid extraction reaction, and the obtained sample solution is filled in the capillary; the sample transfer module may be configured to be used in the sample processing module and the thermal The test tube rack is transferred between circulation modules; the thermal cycling module can be configured to thermally cycle the sample in the capillary; the optical module can include a light source assembly, an imaging assembly and a fiber optic bundle; the light source The assembly may be configured to provide excitation light, the light source assembly irradiating the capillary tube at the thermal cycling module through the fiber optic bundle to excite fluorescence; the fluorescence is irradiated into the An imaging assembly that can be configured to acquire the fluorescence and form a fluorescence imaging photograph.

Optionally, a cassette moving assembly may be provided on the frame; the cassette can be placed on the cassette moving assembly, and the cassette moving assembly is configured to move the cassette to the desired location. at the sample processing module.

Optionally, the cartridge may include a cartridge body and a pipetting mechanism; the cartridge body may be formed with a plurality of compartments, the test tube rack may be connected with the cartridge body, and the test tube rack is A plurality of the capillary tubes can be installed; the pipetting mechanism can be connected with the main body of the cassette, and the sample processing module can act on the pipetting mechanism, so that the pipetting mechanism can be installed in a plurality of the chambers Reagent transfer between the capillary and the capillary.

Optionally, the plurality of chambers include a sample chamber for storing samples to be tested, a waste liquid chamber for storing waste liquid, and a reconstitution chamber for storing reconstituted solution, and the bottom of the cassette has a plurality of flow channels, The plurality of flow passages can conduct corresponding ones of the plurality of chambers.

Optionally, the pipetting mechanism may include a main mechanical valve and an auxiliary mechanical valve; the main mechanical valve and the auxiliary mechanical valve may be rotatably connected to the main body of the cartridge, and the main mechanical valve and the Each of the auxiliary mechanical valves may include a valve body and a valve stem; the valve body of the main mechanical valve can be rotated, so that the valve body of the main mechanical valve is communicated with a plurality of the cabins respectively; The valve stem can perform piston movement to transfer reagents between the valve body of the main mechanical valve and the chamber communicated with it; the valve body of the auxiliary mechanical valve can be rotated to make the auxiliary mechanical valve The valve bodies are respectively connected with a plurality of capillaries on the test tube rack and a plurality of reconstitution chambers in the chambers; the valve stem of the auxiliary mechanical valve can perform piston movement, so that the auxiliary mechanical valve can extract reconstituting the sample solution in the chamber and filling the sample solution into the plurality of capillaries.

Optionally, the sample processing module may include a cartridge operation unit; the cartridge operation unit may include a valve body rotation assembly, a main valve stem lifting assembly and a secondary valve stem lifting assembly; the valve body rotation assembly can be combined with all The main mechanical valve and the valve body of the auxiliary mechanical valve are connected to drive the valve body of the main mechanical valve and the auxiliary mechanical valve to rotate; the main valve stem lifting assembly can be connected with the valve body of the main mechanical valve The stem is connected to drive the valve stem of the main mechanical valve to perform piston movement in the valve body of the main mechanical valve; the auxiliary valve stem lifting assembly can be connected with the valve stem of the auxiliary mechanical valve to drive the The valve stem of the auxiliary mechanical valve performs piston movement in the valve body of the auxiliary mechanical valve.

Optionally, the sample processing module may further include a physical processing unit; the physical processing unit may be disposed below the cartridge body, and the physical processing unit may include a lift drive device, a magnetic suction assembly and an ultrasonic heating assembly ; The magnetic attraction assembly and the ultrasonic heating assembly can be respectively connected with the driving ends of the lift drive device; the lift drive device can be configured to drive the magnetic attraction assembly and the ultrasonic heating assembly to rise and fall, so that the magnetic attraction assembly or the ultrasonic heating assembly abuts against the outer wall of the sample chamber in the plurality of chambers of the cassette; the ultrasonic heating assembly may be configured to be used for performing the sample operation on the sample in the sample chamber. heating and sonication; the magnetic attraction assembly may be configured to attract magnetic beads within the sample chamber.

Optionally, the physical processing unit may further include a translation drive device; the lift drive device may be disposed on the translation drive device, and the translation drive device can drive the lift drive device to move, so as to move the magnetic The suction assembly or the ultrasonic heating assembly is moved below the sample chamber.

Optionally, the sample transfer module may be located between the sample processing module and the thermal cycling module, and the sample transfer module may include a gripper mechanism and a gripper lifting device; the gripper mechanism may be mounted on the On the clamping jaw lifting device, the clamping jaw lifting device can be configured to drive the clamping jaw mechanism to rise and fall; the clamping jaw mechanism can grab the test tube rack, and the clamping jaw mechanism can rotate to drive The test tube rack is turned over at a predetermined angle, and the test tube rack is turned over from the sample processing module to the thermal cycle module.

Optionally, the thermal cycling module may include a moving mechanism, a reverse transcription tank, a high temperature tank, a low temperature tank, and a photodetection tank; the reverse transcription tank, the high temperature tank, the low temperature tank, and the optical detection tank may be side by side The reverse transcription tank, the high temperature tank, the low temperature tank and the optical detection tank may be respectively provided with temperature control elements; the sample transfer module can place the test tube rack in the mobile mechanism, so as to move the test tube rack to the top of the reverse transcription tank, the high temperature tank, the low temperature tank or the photodetection tank by the moving mechanism, and move the test tube rack on the test tube rack The capillary is placed in the reverse transcription tank, the high temperature tank, the low temperature tank or the photodetection tank.

Optionally, the moving mechanism includes a first sliding mechanism, a second sliding mechanism and a support plate, the support plate is slidably connected to the second sliding mechanism, and slides with the first sliding mechanism through the second sliding mechanism connected, so that the first sliding mechanism drives the support plate to move sequentially from the end close to the sample transfer module to the top of the reverse transcription tank, the high temperature tank, the low temperature tank or the photodetection tank ; and the second sliding mechanism is configured to drive the support plate to rise and fall.

Optionally, the optical detection slot may be formed with multiple optical detection cavities, and the multiple optical detection cavities may correspond one-to-one with the multiple capillaries; the sidewall of the optical detection slot may be formed with multiple optical detection cavities A plurality of light-passing holes are opened at opposite positions of the optical detection cavity, and the plurality of light-passing holes can be respectively communicated with the corresponding optical detection cavity, so that the excitation light can be irradiated to the capillary.

Optionally, an optical fiber fixing plate may also be provided on the optical detection slot; the optical fiber fixing plate abuts against the side wall of the optical detection slot on the side where the light-passing hole can be opened, and the optical fiber A plurality of communication holes may be opened on the fixing plate, and the plurality of communication holes may be in one-to-one correspondence with and communicate with the plurality of light-passing holes; the communication holes may be configured to be used for accessing the optical fiber bundle, and each A focusing lens is sandwiched and installed between each of the communicating holes and the corresponding light-passing holes.

Optionally, a heating sheet is provided on the bottom wall of the optical detection tank for heating the optical detection tank, and the optical detection tank is formed with a first heat preservation chamber and a second heat preservation chamber, so The plurality of optical detection chambers may be located between the first thermal insulation chamber and the second thermal insulation chamber, and a thermal insulation layer is provided on the outside of the optical detection tank to keep the optical detection tank warm.

Optionally, the number of the optical fiber bundles may be multiple, and the multiple optical fiber bundles may be in one-to-one correspondence with the multiple capillary tubes; each of the multiple optical fiber bundles may include three ports, and the multiple optical fiber bundles may each include three ports. The first ports of the bundles can all be connected to the light source assembly, the second ports of the multiple optical fiber bundles can be connected to the multiple communication holes respectively, and the third ports of the multiple optical fiber bundles can be connected to each other. The imaging assembly; the excitation light emitted by the light source assembly can enter the first ports of the plurality of optical fiber bundles, and irradiate the corresponding capillary tubes through the second ports of the plurality of optical fiber bundles, so as to excite multiple optical fiber bundles. The fluorescent substances in each of the capillaries emit fluorescence; the fluorescent light can enter the second ports of the plurality of optical fiber bundles, and is irradiated to the imaging assembly through the third ports of the optical fiber bundles.

Compared with the prior art, the beneficial effects of the present application are at least:

Molecular diagnostic platforms provided herein may include racks, cartridges, sample processing modules, sample transfer modules, thermal cycling modules, and optical modules. The sample processing module, the sample transfer module, the thermal cycle module and the optical module can be respectively arranged on the rack; the cassette can be configured to place the sample to be tested and various reagents, and the sample can be prepared with various reagents in the cassette. In order to complete the nucleic acid extraction reaction of the sample, a cassette moving component can be set on the rack, and the operator can place the cassette on the cassette moving component, and then transport the cassette to the sample through the cassette moving component. The work station of the processing module; the sample processing module can be configured to operate the cartridge, so that the reagents in the cartridge react with the sample in a predetermined order to complete the nucleic acid extraction reaction, and the obtained sample solution is filled into the capillary; The sample transfer module is located between the sample processing module and the thermal cycler module. After the nucleic acid extraction of the sample is completed in the cartridge and filled in the capillary, the test tube rack is transferred to the thermal cycler module through the sample transfer module, so that the sample can be stored in the thermal cycler module. PCR reaction is carried out at the place, and fluorescence quantitative detection is performed on the sample in the capillary tube after the PCR reaction has been completed by the optical module, so as to collect the fluorescence signal and obtain the fluorescence imaging photo. Therefore, in the whole detection process, the operator only needs to place the cassette containing the samples and various reagents on the rack, and the subsequent operations can be completed automatically and efficiently through each module.

Description of drawings

In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the accompanying drawings that need to be used in the description of the specific embodiments or the prior art will be briefly introduced below. The drawings are some embodiments of the present application. For those of ordinary skill in the art, other drawings can also be obtained from these drawings without any creative effort.

FIG. 1 is a schematic structural diagram of a molecular diagnostic platform provided in an embodiment of the present application from a first perspective;

2 is a schematic structural diagram of the molecular diagnostic platform provided by the embodiment of the present application from a second perspective;

3 is a schematic structural diagram of a cassette provided by an embodiment of the present application;

FIG. 4 is a schematic structural diagram of a multi-channel fluorescence quantitative detection device provided in an embodiment of the present application from a first viewing angle;

FIG. 5 is a schematic structural diagram of a multi-channel fluorescence quantitative detection device provided in an embodiment of the present application from a second viewing angle;

Figure 6 is a sectional view at A-A in Figure 5;

Fig. 7 is a sectional view at B-B in Fig. 5;

FIG. 8 is a schematic structural diagram of an optical detection slot provided by an embodiment of the present application;

FIG. 9 is a schematic structural diagram of a heating device provided by an embodiment of the present application;

10 is a schematic structural diagram of a heating component in a heating device provided by an embodiment of the present application;

11 is a schematic structural diagram of a sample storage bin provided by an embodiment of the present application from a first perspective;

12 is a schematic structural diagram of a sample storage bin provided by an embodiment of the present application from a second perspective;

13 is a schematic structural diagram of a heating device provided by another embodiment of the present application from a first viewing angle;

FIG. 14 is a schematic structural diagram of a heating device provided in another embodiment of the present application from a second viewing angle;

FIG. 15 is a schematic structural diagram of the heating device provided by another embodiment of the present application from a first perspective during practical application;

FIG. 16 is a schematic structural diagram of the heating device provided in another embodiment of the present application from a second viewing angle during practical application;

FIG. 17 is a schematic structural diagram of a to-be-heated member from a first viewing angle provided by yet another embodiment of the present application;

FIG. 18 is a schematic structural diagram of a to-be-heated member from a second viewing angle according to another embodiment of the present application;

FIG. 19 is a schematic structural diagram of a heating device provided by yet another embodiment of the application;

20 is a schematic structural diagram of a clamping mechanism provided in an embodiment of the application;

21 is a schematic structural diagram of a to-be-clamped member provided in an embodiment of the application;

FIG. 22 is a schematic structural diagram of a clamping mechanism provided in another embodiment of the present application from a first perspective;

FIG. 23 is a schematic structural diagram of a clamping mechanism provided in another embodiment of the present application from a second viewing angle;

FIG. 24 is a schematic structural diagram of the transfer device provided in other embodiments of the present application in a practical application process;

FIG. 25 is a schematic structural diagram of a to-be-clamped member provided in other embodiments of the present application.

Detailed ways

The technical solutions of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present application, but not all of the embodiments.

The components of the embodiments of the present application generally described and shown in the drawings herein may be arranged and designed in a variety of different configurations. Thus, the following detailed description of the embodiments of the application provided in the accompanying drawings is not intended to limit the scope of the application as claimed, but is merely representative of selected embodiments of the application.

Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

In the description of this application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation or a specific orientation. construction and operation, and therefore should not be construed as limitations on this application. Furthermore, the terms "first", "second", and "third" are used for descriptive purposes only and should not be construed to indicate or imply relative importance.

In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it may be a fixed connection or a detachable connection Connection, or integral connection; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be internal communication between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood in specific situations.

The molecular diagnostic platform according to some embodiments of the present application is described below with reference to FIGS. 1 to 3 .

The present application provides a molecular diagnostic platform, as shown in FIG. 1 and FIG. 2 , which may include a rack 1 , a cassette 2 , a sample processing module 3 , a sample transfer module 4 , a thermal cycle module 5 and an optical module 6 .

The cartridge 2 can be configured to place the sample to be tested and various reagents, and the sample can react with various reagents in a predetermined order in the cartridge 2 to complete the nucleic acid extraction reaction of the sample.

Preferably, as shown in FIG. 3 , the cartridge 2 may include a cartridge body, a pipetting mechanism and a test tube rack 23; the cartridge body may be formed with a plurality of compartments, and three of the plurality of compartments may be used as sample compartments, Waste liquid compartment and reconstitution compartment, and the other compartments are used as reagent compartments; the sample to be tested is encapsulated in the sample compartment, and multiple reagent compartments can be filled with different reaction reagents for reacting with the sample, complete Pretreatment of samples; PCR reaction systems in the form of lyophilized beads can be installed in the reconstitution chamber. The test tube rack 23 is detachably mounted on the cartridge body, and a plurality of capillaries 24 can be installed on the test tube rack 23, and the plurality of capillaries 24 contain primers and probes for specific targets stored in freeze-dried or spot-dried form.

The pipetting mechanism is connected to the main body of the cartridge. Through the operation of the pipetting mechanism, the reaction reagents in the multiple reagent chambers can be transferred to the sample chambers for pretreatment reaction with the samples, and the waste liquid produced in each step of the reaction can be transferred to the sample chamber. in the waste tank. After the sample completes the pretreatment reaction in the sample chamber, the pipetting mechanism can transfer the sample to the reconstitution chamber, and after the reconstitution is completed, the sample solution is perfused into the multiple capillaries through the pipetting mechanism.

In this embodiment, preferably, the pipetting mechanism may include a main mechanical valve 21 and an auxiliary mechanical valve 22; the main mechanical valve 21 may be rotatably connected to the cartridge body, and the main mechanical valve 21 may be a piston valve and may include a valve A valve body and a valve stem arranged in the valve body, the valve stem can perform piston movement in the valve body. Rotating the valve body of the main mechanical valve 21 can make the valve body communicate with a plurality of chambers, respectively, and pulling the valve stem can draw the reagent in the chamber connected with the main mechanical valve 21 into the valve body of the main mechanical valve 21, and push the valve stem The reagent in the valve body of the main mechanical valve 21 can be pushed into the chamber communicated with the main mechanical valve 21 .

In the process of pre-processing the samples in the sample chamber, the main mechanical valve 21 can draw the reagents in the multiple reagent chambers into the sample chamber in a predetermined order, so that the samples can be mixed with various reagents such as lysing solution, cleaning solution It reacts with the eluent, etc., and extracts the waste liquid formed after each step of the reaction is completed into the waste liquid tank; after the sample completes a series of reactions, the sample in the sample tank is extracted through the main mechanical valve 21 until it is repeated. Inside the tank.

The auxiliary mechanical valve 22 can be rotatably connected to the main body of the cartridge. The auxiliary mechanical valve 22 can have the same structure as the main mechanical valve 21. Rotating the valve body of the auxiliary mechanical valve 22 can make the auxiliary mechanical valve 22 connect with the reconstitution chamber and the plurality of capillaries 24 respectively. After the reconstitution is completed, by operating the valve stem of the auxiliary mechanical valve 22, the sample solution in the reconstitution chamber can be extracted into the auxiliary mechanical valve 22, and then filled into a plurality of capillaries 24 through the auxiliary mechanical valve 22 in turn. , for subsequent detection steps.

The sample processing module 3 can be configured to operate the main mechanical valve 21 and the auxiliary mechanical valve 22 of the cartridge 2 to realize the automatic operation of the main mechanical valve 21 and the auxiliary mechanical valve 22 to improve the detection efficiency and avoid manual transfer of reagents Errors that may exist in the process improve detection accuracy.

The sample processing module 3 may be disposed on the rack 1 , and one end of the rack 1 may be provided with a cassette moving component, and the cassette moving component may extend to the sample processing module 3 . The operator can place the cassette 2 on the cassette moving assembly, and then transport the cassette 2 to the station of the sample processing module 3 through the cassette moving assembly.

In an embodiment of the present application, preferably, the sample processing module 3 may include a cassette operation unit; as shown in FIG. 2 , the cassette operation unit may include a valve body rotating assembly 31 , a main valve stem lifting assembly 32 and a secondary valve Rod lifting assembly 33, wherein the valve body rotating assembly 31 can act on the valve body of the main mechanical valve 21 and the valve body of the auxiliary mechanical valve 22, and can independently drive the valve body of the main mechanical valve 21 and the valve body of the auxiliary mechanical valve 22 to rotate , so that the main mechanical valve 21 is communicated with a plurality of chambers respectively, and the auxiliary mechanical valve 22 is communicated with the reconstitution chamber and a plurality of capillary tubes 24 . The main valve stem lifting assembly 32 can act on the valve stem of the main mechanical valve 21 to make the valve stem of the main mechanical valve 21 perform a piston movement in the valve body to extract reagents into the valve body of the main mechanical valve 21 or push out the main mechanical valve 21 Reagents in the valve body. The auxiliary valve stem lifting assembly 33 can act on the valve stem of the auxiliary mechanical valve 22, so that the valve stem of the auxiliary mechanical valve 22 performs a piston movement in the valve body, so that the auxiliary mechanical valve 22 can extract the sample solution in the reconstitution chamber, and The sample solution is filled into a plurality of capillaries 24 .

Preferably, the valve body rotating assembly 31 may include a first lifting device, a first rotating device and a second rotating device, both the first rotating device and the second rotating device may be mounted on the first lifting device, and the first rotating device may Located above the main mechanical valve 21 , the second rotating device may be located above the auxiliary mechanical valve 22 .

The first lifting device can drive the first rotating device and the second rotating device to descend to a predetermined position, so that the first rotating device is connected with the valve body of the main mechanical valve 21, and the second rotating device is connected with the valve body of the auxiliary mechanical valve 22. connect. The first rotating device can drive the valve body of the main mechanical valve 21 to rotate, so that the valve body of the main mechanical valve 21 is communicated with the multiple chambers of the cartridge 2 respectively; the second rotating device can drive the valve body of the auxiliary mechanical valve 22 to rotate. , so that the valve body of the auxiliary mechanical valve 22 is communicated with the reconstitution chamber and the plurality of capillaries 24 respectively.

Preferably, the main valve stem lifting assembly 32 and the auxiliary valve stem lifting assembly 33 have the same structure. Taking the main valve stem lifting assembly 32 as an example for illustration, the main valve stem lifting assembly 32 may include a second lifting device and a plug-board telescopic device, The plug-in expansion device can be installed on the second lifting device, and the second lifting device can drive the plug-in expansion device to move to the valve stem of the main mechanical valve 21; the plug-in expansion device can include a plug-in plate and a driving device, and the driving device can Drive the plug-in board to extend toward the valve stem, so that the plug-in board and the valve rod are plugged together; then the plug-in board telescopic device and the valve rod of the main mechanical valve 21 can be driven to rise and fall through the second lifting device, so that the main mechanical The valve stem of the valve 21 performs piston movement in the valve body.

In this embodiment, preferably, as shown in FIG. 2 , the sample processing module 3 may further include a physical processing unit 34 , and the physical processing unit 34 is used to provide suitable reaction conditions for the samples in the sample chamber.

Preferably, the physical processing unit 34 may be disposed below the cartridge 2, and the physical processing unit 34 may include a lift drive device, a translation drive device, a magnetic attraction component and an ultrasonic heating component. The drive end of the translation drive device can be formed with a support platform, and the lift drive device is installed on the support platform of the translation drive device; the drive end of the lift drive device can also be formed with a support platform, and the magnetic attraction component and the ultrasonic heating component are arranged at intervals on the lift drive device. on the support platform of the device.

The translation driving device can drive the magnetic attraction component and the ultrasonic heating component to move in the horizontal direction, so that the magnetic attraction component or the ultrasonic heating component is moved to just below the sample compartment of the cartridge 2 . The lift driving device can drive the magnetic attraction component and the ultrasonic heating component to move upward, so that the magnetic attraction component or the ultrasonic heating component located directly below the sample chamber rises to abut against the outer wall of the sample chamber.

The ultrasonic heating component can heat the samples and reagents in the sample chamber and provide sonic treatment, so that the samples and reagents can be mixed more uniformly, and provide a suitable reaction temperature for the reaction of samples and reagents, so that the reaction between samples and reagents can be more efficient. full.

Magnetic beads can be arranged in the sample compartment, and the magnetic attraction component can adsorb the magnetic beads, so as to adsorb the magnetic beads at a position away from the liquid outlet of the sample compartment, so that when the samples and reagents in the sample compartment are transferred to the sample compartment , to prevent the magnetic beads from flowing out.

As shown in FIG. 2 , the sample transfer module 4 and the thermal cycle module 5 can also be arranged on the rack 1, and the sample transfer module 4 can be located between the sample processing module 3 and the thermal cycle module 5. When the sample is in the cassette 2 After nucleic acid extraction is completed and the capillary tube 24 is filled, the test tube rack 23 is transferred to the thermal cycler module 5 through the sample transfer module 4 , so that the sample is subjected to PCR reaction at the thermal cycler module 5 .

In an embodiment of the present application, preferably, the sample transfer module 4 may include a gripper mechanism and a gripper lifting device, and the gripper mechanism is mounted on the gripper lifting device, so as to drive the gripper mechanism to move to the gripper lifting device through the gripper lifting device. At the position directly opposite the test tube rack 23 horizontally. The gripper mechanism can grip the test tube rack 23 and drive the test tube rack 23 to turn 180 degrees, and transfer the test tube rack 23 from the sample processing module 3 on one side of the gripper mechanism to the thermal cycle module 5 on the other side. The capillary 24 is turned over 180 degrees, from the previous state of upside down and the test tube rack 23 to the state where the capillary 24 is facing upward.

In an embodiment of the present application, preferably, as shown in FIG. 2 , the thermal cycle module 5 may include four independent thermal tanks, which are a photodetection tank 52 , a reverse transcription tank 53 , a low temperature tank 54 and a high temperature tank 55 , respectively. , the four heat sinks can be arranged side by side on the rack 1 at intervals, and temperature control elements can be respectively arranged on the four heat sinks to independently control the temperature of the four heat sinks.

The thermal cycle module 5 may further include a moving mechanism 51 , the moving mechanism 51 is disposed across the four thermal tanks, and one end of the moving mechanism 51 extends to the sample transfer module 4 . In this embodiment, preferably, the moving mechanism 51 may include a first sliding mechanism, a second sliding mechanism and a support plate, the support plate is slidably connected to the second sliding mechanism, and is slidably connected to the first sliding mechanism through the second sliding mechanism , so that the first sliding mechanism can drive the support plate to move sequentially from the end close to the sample transfer module 4 to the top of the four heat sinks; the second sliding mechanism can be configured to drive the support plate to move up and down.

In the initial state, the support plate is located near one end of the sample transfer module 4, and the second sliding mechanism moves the support plate to a position of an appropriate height, so that the sample transfer module 4 turns the test tube rack 23 over to the position facing the thermal cycle module 5. On one side, the capillary tube 24 can be placed on the support plate through the test tube rack 23 to complete the transfer of the capillary tube 24 between the sample processing module 3 and the thermal cycle module 5 . Then, the first sliding mechanism drives the capillary 24 to move to the top of the reverse transcription tank 53, the high temperature tank 55, the low temperature tank 54 and the photodetection tank 52 in turn, and put the capillary 24 into the reverse transcription tank 53, the high temperature tank 55, and the low temperature tank 54. And in the photodetection tank 52, the four thermal tanks are respectively equipped with stable control elements, so that the four thermal tanks have different temperatures, so that the samples in the capillary 24 are sequentially subjected to reverse transcription reaction in the reverse transcription tank 53, and high temperature tank 55. The DNA degradation reaction is carried out in the low temperature tank 54, and the fluorescence quantitative detection is carried out in the optical detection tank 52 after the temperature reduction and annealing in the low temperature tank 54, so as to complete a PCR reaction. Since four independent heat baths are set, the temperature of the four heat baths can be controlled independently, avoiding the design of the PCR thermal cycle system using temperature gradients, so that the samples can quickly complete the PCR reaction of 40 cycles, and the 40 cycles of the PCR reaction can be completed quickly. PCR reactions can be completed within 5-8 minutes.

In this embodiment, preferably, the fluorescent quantitative detection of the sample solution in the capillary 24 is completed in the optical detection tank 52 , and the optical detection tank 52 can be formed with multiple optical detection cavities, and the multiple optical detection cavities can be combined with the multiple capillary tubes 24 . One-to-one correspondence, so that the plurality of capillaries 24 can be respectively placed in the corresponding optical detection chambers; at the same time, the temperature of the optical detection tank 52 is controlled by the temperature control element disposed on the optical detection tank 52, so that the sample in the capillary tube 24 is controlled. Always be at a suitable temperature when performing fluorescence quantitative detection. As shown in FIG. 8 , a plurality of light-passing holes 523 may be opened at positions opposite to the plurality of light-checking cavities on the sidewall of the light-detecting slot, and the plurality of light-passing holes 523 may be respectively communicated with the corresponding light-checking cavities, so as to The capillary 24 is irradiated with excitation light emitted from the optical module 6 . The side wall of the optical detection slot 52 can also be provided with an optical fiber fixing plate 521, and the optical fiber fixing plate 521 abuts against the side wall of the side where the light-passing hole 523 is opened in the optical detection slot 52, and the optical fiber fixing plate 521 can be provided with an optical fiber fixing plate 521. A plurality of communication holes (or a plurality of optical fiber fixing holes) 522, the plurality of communication holes 522 are in one-to-one correspondence with the plurality of light-passing holes 523 and communicated, and each communication hole 522 and the light-passing hole 523 are sandwiched between one focusing lens.

The optical module 6 is used to perform quantitative fluorescence detection on the capillary 24 in the optical detection tank 52. In an embodiment of the present application, preferably, as shown in FIG. 4, the optical module 6 may include a light source assembly 61, an imaging assembly 62 and Optical fiber bundles; the optical fiber bundles are three-port optical fiber bundles, and the number of optical fiber bundles is multiple, and the multiple optical fiber bundles are in one-to-one correspondence with the multiple capillaries 24; the first ports of the multiple optical fiber bundles converge into a single access light source assembly 61, The second ports of the multiple optical fiber bundles are respectively connected to the multiple optical fiber fixing holes 522 of the optical fiber fixing plate 521 , and the third ports of the multiple optical fiber bundles are aggregated and connected to the imaging assembly 62 . Wherein the light source assembly 61 can provide incident light with different wavelength bands, preferably, the light source assembly 61 may include an LED lamp, a collimating lens, a filter wheel equipped with filters, and a focusing lens; the imaging assembly 62 may include a camera, a focusing lens , collimating lens and imaging assembly 62 also includes a filter wheel with filters. In the light source assembly 61, the LED lamp can emit white light, the white light is filtered into monochromatic light with a predetermined wavelength band after being filtered by a collimating lens and a filter, and enters the first port of a plurality of fiber bundles through a focusing lens; and then passes through a plurality of fibers. The second port of the beam emits and irradiates the corresponding plurality of capillaries 24 to excite the fluorescent substances in the capillaries 24 to emit fluorescence in corresponding wavelength bands. The fluorescence excited by the plurality of capillaries 24 then enters the second port of the corresponding optical fiber bundle, and then irradiates into the imaging assembly 62 from the third port; After the film and the camera lens enter the camera, the fluorescence is collected by the camera and the fluorescence imaging photo is taken.

The filter wheels of the light source assembly 61 and the imaging assembly 62 may include a plurality of filters and correspond one-to-one, one filter of the light source assembly 61 and one filter of the corresponding imaging assembly 62 are a group, Thus, a plurality of filter sets are formed; in the process of performing fluorescence quantitative detection on the capillary 24, the plurality of filter sets can be rotated to a position that allows light to pass through, so that the capillary 24 can be subjected to excitation light of different wavelength bands. Illuminate and form corresponding fluorescence imaging pictures.

Therefore, through the sample processing module 3, the sample transfer module 4, the thermal cycle module 5 and the optical module 6 on the rack 1, the cartridge 2 containing the sample and various reagents only needs to be placed at the sample processing module 3, Subsequent operations can be completed automatically and efficiently through each module.

In some embodiments, the optical module 6 of the molecular diagnostic platform may be a multi-channel fluorescence quantitative detection device. The multi-channel fluorescence quantitative detection device according to some embodiments of the present application is described below with reference to FIGS. 4 to 8 .

In some embodiments, the fluorescence quantitative detection device of the present application is used to perform fluorescence quantitative detection on the sample in the capillary; as shown in FIG. 4 , the fluorescence quantitative detection device may include a first rack 11 , a light source assembly 61 , a first filter The optical component 13 , the second optical filter component 14 , the imaging component 62 , the optical fiber bundle and the optical detection slot 52 .

The optical detection tank 52 can be placed on the first rack 11, and the optical detection tank 52 can be used to fix the capillary tube containing the sample to be detected. The number of capillaries may be multiple, and the multiple capillaries may be arranged side by side in the photodetection groove 52 at intervals.

The light source assembly 61 can provide a white light source, the light source assembly 61 and the first filter assembly 13 can be disposed opposite to the first frame 11, and the white light source emitted by the light source assembly 61 can illuminate the first filter assembly 13, and then pass through the first filter assembly 13. A filter component 13 filters the white light source, so that monochromatic light of a predetermined wavelength band passes through the first filter component 13 to form incident light with a predetermined wavelength band for illuminating the capillary.

The fiber bundle may be a three-port fiber bundle, including a first port, a second port and a third port, an incident fiber may be formed between the first port and the second port, and an exit fiber may be formed between the second port and the third port . The number of optical fiber bundles can be multiple, and multiple optical fiber bundles can be in one-to-one correspondence with multiple capillaries. The formed incident light can enter into the plurality of incident optical fibers uniformly through the plurality of first ports.

The second ports of the multiple optical fiber bundles can be respectively connected to the optical detection slot 52, and the multiple second ports are respectively directed toward the corresponding capillaries. The incident light can be transmitted to the corresponding second port through a plurality of incident optical fibers, and then the incident light of the predetermined wavelength band is irradiated on the corresponding capillary tube through the second port, so as to excite the fluorescent substance in the capillary tube to emit fluorescence in the corresponding wavelength band.

The second filter assembly 14 can be disposed on the first rack 11, and the third ports of the multiple fiber bundles can be converged into a single bundle to be connected to the second filter assembly 14, so that the fluorescence of the corresponding wavelength band emitted by the multiple capillaries can be irradiated on the On the second filter component 14; the imaging component 62 can be disposed opposite to the second filter component 14, the fluorescence can be irradiated on the imaging component 62 through the second filter component 14, and the stray light can be removed by the second filter component 14, thereby The fluorescence imaging photos of multiple capillaries are conveniently and quickly obtained through the imaging component 62 to analyze the concentration of the sample and improve the efficiency of fluorescence quantitative detection of the sample.

In some embodiments of the present application, preferably, as shown in FIGS. 4 to 6 , the first filter component 13 can filter the white light source emitted by the light source component 61 to form a variety of monochromatic lights with different wavelength bands .

Preferably, as shown in FIG. 6 , the first filter assembly 13 may include a filter wheel 131, a filter 132, a driving device 133 and a light shield 134; the driving device 133 may be fixedly arranged on the first frame 11, The driving end of the driving device 133 can be connected with the filter wheel 131 to drive the filter wheel 131 to rotate around the axis of the filter wheel 131 through the driving device 133 . The number of the filters 132 may be multiple, the multiple filters 132 may be distributed at intervals along the circumferential direction of the filter wheel 131 , and the multiple filters 132 can respectively pass light of different wavelength bands. The light blocking cover 134 can be covered on the filter wheel 131 , a light inlet can be opened on the side wall of the light blocking cover 134 on the side opposite to the filter wheel 131 , and the side wall on the other side of the light blocking cover 134 can be opened. A light outlet can be opened, and the light inlet and the light outlet can be located on the same axis. The driving device 133 can drive the filter wheel 131 to rotate, so that the plurality of filters 132 rotate between the light inlet and the light outlet in turn, and are located on the same axis as the light inlet and the light outlet, so that the light inlet and the light outlet are on the same axis. The optical path is formed between the ports.

The white light source emitted by the light source assembly 61 can be irradiated on the filter 132 located between the light inlet and the light outlet through the light inlet, and then the white light source is filtered by the filter 132, so that the monochromatic light of the corresponding wavelength band passes through. The filter 132 is emitted through the light outlet to form incident light with a predetermined wavelength band for irradiating the capillary.

In some embodiments, preferably, the structure of the second optical filter assembly 14 may be the same as that of the first optical filter assembly 13 , which will not be repeated here; The number of the filters 132 on the filter assembly 13 is the same and corresponds to each other one by one.

Preferably, the number of filters 132 on the first filter assembly 13 may be four, and the four filters 132 are respectively a red filter, a yellow filter, a green filter and a blue filter ; the red filter is used to pass red light at 607-644nm, the yellow filter is used to pass yellow light at 552-594nm, the green filter is used to pass green light at 509-545nm, the blue Color filters are used to pass blue light at 420-490 nm.

When the incident light of these four wavelength bands is irradiated on the capillary, the fluorescent substance in the capillary can excite the fluorescence of the corresponding wavelength band, which are red fluorescence, yellow fluorescence, green fluorescence and blue fluorescence respectively. Correspondingly, the number of filters on the second filter assembly 14 may also be four, and the four filters are respectively a red fluorescence filter, a yellow fluorescence filter, a green fluorescence filter and a blue fluorescence filter. Filters; red fluorescence filter is used to pass red fluorescence at 644-686nm, yellow fluorescence filter is used to pass yellow fluorescence at 594-634nm, green fluorescence filter 132 is used to pass 545-583nm The green fluorescence passes through, and a blue fluorescence filter is used to pass blue fluorescence from 490-532 nm.

In the specific use process, taking the red incident light as an example, when the red incident light needs to be used to irradiate the capillary, the red filter and the red fluorescence filter are respectively rotated to the corresponding optical path channels, so that the light source emits The white light source is filtered by the first filter component 13 to form incident light of 607-644nm, and is covered on the capillary tube, and then the fluorescent substance in the capillary tube is excited to emit red fluorescence; after the red fluorescence is filtered by the second filter component 14, The red fluorescence of 644-686 nm is irradiated into the imaging component 62, and the imaging component 62 is used to obtain a fluorescence imaging photo of a plurality of capillaries formed under the excitation of the incident light of 607-644 nm.

In some embodiments of the present application, preferably, the driving devices 133 of the first filter assembly 13 and the second filter assembly 14 may both be stepper motors, so that the corresponding filter wheels can be precisely controlled by the stepper motors 131 to rotate the required filter 132 to the optical path.

Preferably, as shown in FIG. 4 , a photoelectric sensor 136 may be provided at the stepper motors of the first filter assembly 13 and the second filter assembly 14 respectively; one end of the output shaft of the stepper motor may be connected to the filter wheel 131 In connection, the other end of the output shaft may be provided with an induction sheet 135 , so that the induction sheet 135 can rotate at the same angle synchronously with the filter wheel 131 .

The edge of the sensing sheet 135 can extend into the photoelectric sensor 136, and the edge of the sensing sheet 135 can be provided with a notch. When the sensing sheet 135 is rotated until the notch is located in the sensing area of the photoelectric sensor 136, the filter wheel 131 is in the initial position Then, based on the initial position, the filter wheel is driven by a stepping motor to rotate at different angles, so that the four filters 132 can be rotated to the optical path in turn.

In some embodiments of the present application, preferably, as shown in FIG. 6 , the light source assembly 61 may include an LED lamp 121 , a light source sleeve 122 , a collimating lens 123 and a heat sink 124 . The light source sleeve 122 can be fixedly arranged on the first frame 11, and the light source sleeve 122 can be located on the side of the first filter assembly 13 where the light inlet is opened; The light inlets are coaxially arranged, one end of the light source sleeve 122 away from the first filter assembly 13 can be connected to the LED lamp 121 , and the other end of the light source sleeve 122 can extend into the light source through the light inlet of the first filter assembly 13 . inside the light shield 134 and extend to a position close to the filter wheel 131 . The collimating lens 123 can be installed in the light source sleeve 122, the LED lamp 121 can emit white light, and the white light emitted by the LED is corrected by the collimating lens 123 to form a beam of parallel light; the parallel light irradiates the filter located on the optical path channel The incident light in the predetermined wavelength band is then filtered by the filter 132 to be used to excite the fluorescent substance in the capillary.

In some embodiments of the present application, preferably, as shown in FIG. 6 , a first optical fiber lens assembly 16 may also be disposed on the side of the first optical filter assembly 13 on which the light outlet is opened; the first optical fiber lens assembly 16 may include The first sleeve 161 , the first focusing lens 162 and the first fiber holder 163 .

The first sleeve 161 can be fixedly arranged on the first frame 11, and the first sleeve 161 can be arranged coaxially with the light outlet of the first filter assembly 13; one end of the first sleeve 161 can pass the first filter The light outlet of the assembly 13 extends into the light shield 134 of the first filter assembly 13 and extends to the filter wheel 131, and the other end of the first sleeve 161 can be connected to the first optical fiber fixing member 163; a plurality of optical fibers After the first ports of the bundle are converged, they can be inserted into the first sleeve 161 through the first optical fiber fixing member 163, the first focusing lens 162 can be installed in the first sleeve 161, and the first port of the optical fiber bundle can be inserted into the first sleeve 161. is located on the focal plane of the first focusing lens 162; the incident light of the predetermined wavelength band obtained after being filtered by the first filter component 13 can be uniformly dispersed into the first ports of the multiple fiber bundles after being focused by the first focusing lens 162, Then, it is transmitted to the corresponding second port through the incident fibers of the multiple fiber bundles, and then the incident light of the predetermined wavelength band is irradiated on the corresponding capillary tube through the second port, so as to excite the fluorescent substances in the multiple capillary tubes to generate Fluorescence in the corresponding band.

In some embodiments of the present application, preferably, as shown in FIG. 7 , a second optical fiber lens assembly 17 may be provided on the side of the second optical filter assembly 14 on which the light inlet is opened, and the second optical fiber lens assembly 17 may include The second sleeve 171 , the second lens 172 and the second fiber holder 173 . The second sleeve 171 can be fixedly disposed on the first frame 11, and the second sleeve 171 can be disposed coaxially with the light inlet of the second filter assembly 14; one end of the second sleeve 171 can pass through the second filter element 14. The light inlet of the optical component 14 extends into the light shield of the second optical filter component 14 and extends to the filter wheel of the second optical filter component 14, and the other end of the second sleeve 171 can be fixed with the second optical fiber piece 173 is connected. After the third ports of the multiple optical fiber bundles converge into one, they can be inserted into the second sleeve 171 through the second optical fiber fixing member 173, and the second lens 172 can be installed in the second sleeve 171; the fluorescence emitted by the multiple capillaries It can enter the outgoing fibers of the multiple optical fiber bundles through the second port of the corresponding optical fiber bundle, and then transmit to the third port of the multiple optical fiber bundles through the multiple outgoing fibers, and send out the capillary through the multiple third ports. The fluorescent light is irradiated on the second lens 172; the second lens 172 is used for correcting the fluorescent light to obtain a parallel fluorescent light beam, so that the parallel fluorescent light beam is irradiated on the filter of the second filter assembly 14 , to filter the fluorescent light beam through the filter of the second filter component 14, so that the fluorescent light beam of a predetermined wavelength band is irradiated into the imaging component 62, so as to obtain a fluorescent imaging photo.

In some embodiments of the present application, preferably, as shown in FIG. 4 and FIG. 7 , the imaging assembly 62 may be disposed on the side of the second filter assembly 14 where the light outlet is opened, and the imaging assembly 62 may include the camera 151 , the first A lens 153 and a camera lens sleeve 152, the first lens 153 can be installed in the camera lens sleeve 152, and the first lens 153 can be coaxially disposed with the camera lens sleeve 152; the camera lens sleeve 152 can be fixedly disposed in the first lens sleeve 152. On a frame 11, and the camera lens sleeve 152 can be coaxially disposed with the light outlet of the second filter assembly 14, one end of the camera lens sleeve 152 can extend into the second filter assembly 14 through the light outlet of the second filter assembly 14. Inside the light shield of the filter assembly 14 and extending to the filter wheel. Preferably, the first lens 153 and the second lens 172 on both sides of the second filter assembly 14 may be achromatic doublet lenses; the camera 151 may be fixedly arranged on the first frame 11, and the camera lens sleeve 152 The other end can be connected to the camera 151, so that the fluorescence emitted by the third port of the fiber bundle passes through the second lens 172, the second filter assembly 14 and the first lens 153 in sequence and then irradiates into the CMOS chip of the camera 151, so that there is no need to The camera lens can collect fluorescence signals through the camera 151 and obtain fluorescence imaging photos.

In some embodiments of the present application, preferably, as shown in FIG. 8 , the optical detection slot 52 may be formed with multiple independent optical detection cavities, the multiple optical detection cavities may be arranged side by side at intervals, and the multiple optical detection cavities may be One-to-one correspondence with multiple capillaries. The upper end of the optical detection tank 52 may be an open end, and the open end is covered with a silicone cover plate 84 , so that the plurality of optical detection cavities are sealed by the silicone cover plate 84 . A capillary insertion hole may be formed on the silicone cover plate 84 at a position corresponding to each optical detection cavity, so that a plurality of capillaries can be inserted into the corresponding optical detection cavity through the capillary insertion hole.

A light-passing hole 523 may be respectively opened on a side wall of one side of the light-detecting groove 52 at a position corresponding to each light-checking cavity, and the light-passing hole 523 may face one end of the capillary tube containing the sample. An optical fiber fixing plate 521 can be provided on the side wall of the optical detection slot 52 on which the light-passing hole 523 is opened. A plurality of optical fiber fixing holes 522 can be opened on the optical fiber fixing plate 521. The light-passing holes 523 are in one-to-one correspondence, and a corresponding group of light-passing holes 523 and the optical fiber fixing holes 522 may be located on the same axis. The second ports of the plurality of optical fiber bundles can be respectively inserted into the plurality of optical fiber fixing holes 522, and a second focusing lens can be arranged between each group of light-passing holes 523 and the optical fiber fixing holes 522, so that the second focusing lens of the optical fiber bundles is second. The incident light emitted from the port is focused by the corresponding second focusing lens and then irradiated on the corresponding capillary tube, so as to excite the fluorescent substance in the capillary tube to emit fluorescence.

In some embodiments, preferably, a heating plate may be provided on the bottom wall of the photodetection slot 52, and the photodetection slot 52 can be heated by the heating plate, so that the photodetection cavity inside the photodetection slot 52 is at a suitable temperature , so as to ensure the accuracy of fluorescence quantitative detection.

Preferably, in order to further ensure that the multiple photodetection chambers are at the same temperature, a first heat preservation chamber and a second heat preservation chamber may be formed in the photodetection tank 52, and the first heat preservation chamber may be located in the multiple photodetection chambers One end of the plurality of photodetection chambers, the second heat preservation chamber may be located at the other end of the plurality of photodetection chambers, so as to prevent the temperature of the two photodetection chambers located at both ends from being too low in the plurality of photodetection chambers.

Preferably, a thermal insulation layer may be provided outside the optical detection tank 52 to further insulate multiple optical detection cavities, ensure that the temperature in the optical detection tank 52 is within a stable range, and improve the accuracy of fluorescence quantitative detection.

According to the fluorescence quantitative detection device of the exemplary embodiment of the present application, the fluorescence imaging photos of multiple capillaries can be obtained conveniently and quickly through the imaging component, so as to analyze the concentration of the sample and improve the efficiency of the fluorescence quantitative detection of the sample.

In some embodiments of the present application, the molecular diagnostic platform may include a heating device, and the heating device is used in the molecular detection technology to heat the sample storage compartment 1000 in the cartridge 2, and the sample storage compartment in the cartridge 2 is heated. 1000 is subjected to heat treatment, for example, including proteinase K treatment, which usually requires a temperature of 55-60 degrees Celsius; the heating device provided in some embodiments of the present application is used to heat the sample storage compartment 1000. As shown in FIG. 12 and FIG. 13 , the The bottom of the sample storage bin 1000 has heating chambers 1010 arranged at intervals, wherein the longitudinal section of the heating chambers 1010 is a trapezoid.

In some embodiments, as shown in FIG. 9 , the heating device may include a heating component and a heat-conducting sleeve 1030; a heat-conducting sleeve 1030 may be sleeved on the heating end of the heating component; wherein, the heat-conducting sleeve 1030 may be connected with the heat-conducting sleeve 1030. Specifically, in order to satisfy the heating cavity 1010 with a trapezoidal longitudinal section, the thermally conductive sleeve 1030 preferably has a hexahedral structure, and one of its end faces is an inclined surface, and the inclined surface can match the inclined surface in the heating cavity 1010. More specifically, considering that the space of the heating cavity 1010 is small, in order to improve the heating efficiency of the heating assembly, one end face of the heat conducting sleeve 1030 is set as an inclined surface, and the inclined surface is adapted to the inclination of the accommodating cavity. There is a larger contact area between the surfaces, thereby improving the heating efficiency of the heating assembly for the sample storage bin 1000 .

It is worth noting that, for the heating cavity 1010 whose longitudinal cross section is a trapezoid, a description is given that the thermally conductive sleeve 1030 has a hexahedral structure; if the structure of the heating cavity 1010 is other polyhedral structures, the structure of the thermally conductive sleeve 1030 can also be It is changed accordingly, as long as it can fit with the inner wall of the heating chamber 1010 .

In the actual heating process, as shown in FIG. 12 and FIG. 13 , first, the heating assembly can heat the heat-conducting sleeve 1030 to a set temperature; then, the heating end of the heating assembly can cooperate with the heat-conducting sleeve 1030 to be inserted into the heating cavity 1010 , so that the thermally conductive sleeve 1030 is closely attached to the end face of the heating cavity 1010, thereby improving the heating efficiency of the heating assembly; overcoming the inability to achieve close contact between the heating sheet 1040 and the heating cavity 1010 in the related art, resulting in time-consuming heating Long, low heating efficiency technical problems.

In this embodiment, in order to ensure a close fit between the thermally conductive sleeve 1030 and the heating cavity 1010, thereby improving the heating efficiency of the heating assembly 102, the thermally conductive sleeve 1030 may be formed of a silicone material; specifically, a thermally conductive sleeve made of silicone material The 1030 has the characteristics of thermal expansion and contraction. When the thermally conductive sleeve 1030 is heated, the heated thermally conductive sleeve 1030 will expand, thereby making the thermally conductive sleeve 1030 closely fit with the heating cavity 1010, thereby improving the heating efficiency of the heating assembly.

Referring to FIG. 10 , in this embodiment, in order to ensure the tight fit between the heat conducting sleeve 1030 and the heating cavity 1010 and thereby improve the heating efficiency of the heating assembly, the heating assembly may include a heating sheet 1040 and a patch type The thermistor is welded to the temperature sensor 1050 on the heating sheet 1040; the thermally conductive sleeve 1030 can be sleeved on the heating sheet 1040, and the heating sheet 1040 can include a substrate 1060 and a heating wire 1070; the heating wire 1070 may be disposed on the substrate 1060 .

On the one hand, since the thermally conductive sleeve 1030 made of silicone material has the characteristics of thermal expansion and contraction, when it is heated, the heated thermally conductive sleeve 1030 will expand; During the process, the cooperation between the heating sheet 1040 and the heat-conducting sleeve 1030 will produce a small amount of outward deformation, which can make the heat-conducting sleeve 1030 and the heating cavity 1010 closely fit, thereby improving the heating efficiency of the heating assembly.

In addition, considering the small volume of the heating chamber 1010, the heating sheet 1040 should be made as thin as possible. Based on this, the substrate 1060 can be made of stainless steel, and the heating wire 1070 can be a thick film heating wire. The thick-film heating wire can be printed on the substrate 1060; by using the printing technology, on the one hand, the thickness of the heating sheet 1040 is thin, on the other hand, it does not affect the heating of the heating wire 1070, and the heat conduction effect is better.

Another embodiment of the heating device of the present application will be described below. This other embodiment is an improvement on the basis of the above-mentioned embodiment. The technical content disclosed in the above-mentioned embodiment will not be described repeatedly, and the content disclosed in the above-mentioned embodiment also belongs to This other embodiment discloses the content.

In another embodiment, as shown in FIG. 13 , the heating device may further include a first driving assembly 1080 , and the driving end of the first driving assembly 1080 may be connected to the heating assembly through a supporting member 1090 , so The first driving assembly 1080 can drive the heating assembly to move along the first direction 1100, specifically, the first direction 1100 is the vertical direction when the heating device is in normal use; so that the heating assembly can be inserted or pulled out in the heating chamber 1010 .

Specifically, the support member 1090 may include a first support plate 1170, a guide block 1180 and a second support plate 1190; wherein the guide block 1180 may be provided with a first support plate 1170, and the first support plate 1170 may be provided on the guide block 1180. The plate 1170 may extend along the first direction 1100, the second support plate 1190 may be disposed on one side of the first support plate 1170 and extend along the second direction 1160 (the second direction 1160 refers to the horizontal direction), The heating assembly may be disposed on the second support plate 1190 .

More specifically, the first drive assembly 1080 may include a first drive member 1110 and a first lead screw; the first drive member 1110 is preferably a first motor, and the first motor may be disposed on the first support plate At the top of 1170, the output shaft of the first motor can be connected with the first lead screw, and the second bearing plate 1190 can be sleeved on the first lead screw and be screwed with the first lead screw .

15 and 16, in the actual driving process, the cartridge 2 can be slidably arranged above the heating device through the second rail 1280. When the sample storage compartment 1000 needs to be heated, first the cartridge 2 can be Move to the preset position, and then the first motor can drive the second support plate 1190 to move vertically upward along the first direction 1100 through the first lead screw, at this time, the heating assembly can be vertically moved along the first direction 1100 Move straight up and insert into the heating chamber 1010 , after heating the cartridge 2 , the first motor can drive the heating assembly to move in a vertical downward direction, so that the heating assembly is pulled out from the heating chamber 1010 .

With reference to FIG. 13 , the end surface of the first supporting plate 1170 facing the second supporting plate 1190 may be provided with a first rail 1200 , and the end surface of the second supporting plate 1190 facing the first supporting plate 1170 may be provided with There is a slider 1210 . When the first motor drives the second support plate 1190 to move in the first direction 1100 , the slider 1210 can move on the first track 1200 to assist the movement of the second support plate 1190 .

Specifically, with reference to FIG. 14 , the first drive assembly 1080 may further include a first limit switch assembly 1310, and the first limit switch assembly 1310 may include a first optocoupler 1320 and a first trigger member 1330; wherein, The first optocoupler 1320 can be disposed on the guide block 1180, and the first trigger member 1330 can be disposed on the second support plate 1190; more specifically, the first optocoupler 1320 can include a signal receiving device terminal and the signal transmitting terminal, and the signal receiving terminal can be used to receive the signal of the signal transmitting terminal, when the first motor drives the heating assembly to move in the vertical downward direction, and when the first trigger 1330 triggers the first When the optocoupler 1320 is used (the first trigger 1330 is located between the signal receiving end and the signal transmitting end), when the signal receiving end cannot receive the signal emitted by the signal transmitting end, it is used to control the first trigger The first controller for motor movement may control the first motor to stop movement, so as to stop the movement of the heating assembly.

In this embodiment, as shown in FIG. 13 , the heating device may further include a second drive assembly 1130; wherein, the second drive assembly 1130 may include a second drive member 1140 and a second lead screw 1150; The driving member 1140 is preferably a second motor, the output shaft of the second motor can be connected with the second lead screw 1150, and the guide block 1180 can be sleeved on the second lead screw 1150 and connected with the second lead screw 1150. Lead screw 1150 threaded connection.

During the actual driving process, the cartridge 2 can be slidably arranged above the heating device through the second rail 1280. When the sample storage compartment 1000 needs to be heated, the cartridge 2 can be moved to a preset position first, which may be a distance from the predetermined distance. There will be a certain error in setting the position. At this time, the second driving assembly 1130 is used to fine-tune the heating assembly in the second direction 1160, so that the heat conduction sleeve 1030 can be inserted into the heating cavity 1010. Specifically, the second motor can drive the guide block 1180. Moving along the second direction 1160 enables the heating assembly to also move along the second direction 1160 , so as to move the heating assembly to a position where the heating chamber 1010 is directly opposite.

Specifically, with reference to FIG. 14 , the second drive assembly 1130 may further include a second limit switch assembly 1340, and the second limit switch assembly 1340 may include a second optocoupler 1350 and a second trigger member 1360; wherein, The second optocoupler 1350 can be disposed on the guide block 1180, and the second trigger 1360 can be disposed on the side plate 1370 (the side plate 1370 is disposed on one side of the heating assembly); more specifically, the The second optocoupler 1350 may include a signal receiving end and a signal transmitting end, and the signal receiving end may be used to receive the signal of the signal transmitting end. When the second motor drives the heating assembly to move in the horizontal direction, and when the second trigger 1360 When the second optocoupler 1350 is triggered (the second trigger 1360 is located between the signal receiving end and the signal transmitting end), when the signal receiving end cannot receive the signal emitted by the signal transmitting end, it is used for The second controller that controls the movement of the second motor may control the movement of the second motor to stop, so as to stop the movement of the heating assembly 102 .

Another embodiment of the present application will be described below. This further embodiment is an improvement on the basis of the above-mentioned embodiment. The technical content disclosed in the above-mentioned embodiment will not be described repeatedly, and the content disclosed in the above-mentioned embodiment also belongs to this further embodiment. Example disclosure.

Another embodiment of the present application provides a component to be heated. As shown in FIG. 17 and FIG. 18 , the component to be heated may be a cassette 2 , and the cassette 2 may have multiple compartments, such as for storing waste liquid. The waste liquid tank 1390, the reconstitution tank 1400 for storing the reconstituted solution, and the alcohol cleaning liquid tank 1410 for storing the alcohol cleaning liquid, etc.; the bottom of the cartridge 2 has a plurality of flow channels 1380. 1380 can turn on the corresponding compartments mentioned above.

18, the bottom of the cartridge 2 can have a heating chamber 1010. When the cartridge 2 is applied in the field of molecular detection technology, the cartridge 2 needs to be heated (for example, proteinase K treatment, usually requires 55-60 Celsius); the heating chamber 1010 can accommodate the heating device in any of the above-mentioned embodiments, and can be adapted to the thermally conductive sleeve 1030 in the above-mentioned heating device, when the above-mentioned heating device is inserted into the heating chamber 1010 When inside the heating chamber 1010, the side wall of the heating chamber 1010 can be attached to the thermally conductive sleeve 1030, and the cartridge 2 can be heated by using the heating device.

Specifically, considering that the volume of the cartridge 2 is small and the space occupied by the heating chamber 1010 is limited, in order to ensure that the heating efficiency of the cartridge 2 can be improved through the heating chamber 1010 with limited space, preferably, the heating chamber 1010 The side wall may have at least one inclined inclined surface, and the inclined inclined surface can be adapted to the heat conduction sleeve 1030 having the inclined inclined surface, so as to increase the heat conduction area between the heating component and the heating cavity 1010, thereby improving the heating efficiency.

Preferably, the bottom of the cartridge 2 can be provided with two heating chambers 1010 symmetrical about the central axis of the cartridge 2. On the one hand, it conforms to the structural arrangement of the cartridge 2, and on the other hand, it further improves the heating of the cartridge 2. efficiency.

Next, another embodiment of the present application will be described. This further embodiment is an improvement on the basis of the above-mentioned embodiment. The technical content disclosed in the above-mentioned embodiment will not be described repeatedly, and the content disclosed in the above-mentioned embodiment also belongs to this still another embodiment. Example disclosure.

In yet another embodiment of the present application, as shown in FIG. 19 , a magnetic attraction member 1220 may be disposed on the second support plate 1190 , and the magnetic attraction member 1220 may include a magnetic attraction member 1220 disposed on the second support plate 1190 . The support rod 1300 on the plate 1190 and the magnet 1290 disposed on the support rod 1300, the support rod 1300 can extend along the first direction 1100, so that the magnet 1290 and the heating assembly in the static state are located on the same horizontal plane .

Specifically, during the nucleic acid extraction process, the magnet 1290 can absorb the magnetic beads of the solution in the cartridge 2. In order to prevent the incomplete adsorption caused by the uneven magnetic force of the magnet 1290, the magnet 1290 can be set on the second support through the support rod 1300. On the plate 1190, that is, when the second motor drives the second support plate 1190 to move in the second direction 1160, the magnet 1290 can move left and right along the bottom of the sample storage compartment, which can fully adsorb the magnetic beads in the solution, and can put the magnetic beads in the solution. The magnetic beads move to a position where they are not easily washed away by the liquid.

The heating assembly provided according to the embodiment of the present application can heat the heat-conducting sleeve to a set temperature; then, the heating end of the heating assembly is inserted into the heating cavity in cooperation with the heat-conducting sleeve, so that the heat-conducting sleeve is closely attached to the end face of the heating cavity, thereby The heating efficiency of the heating assembly is improved, and the technical problems of the related art that the heating sheet and the heating cavity cannot be in close contact, resulting in long heating time and low heating efficiency are overcome.

According to the heating element to be heated provided by the embodiment of the present application, the bottom has a flow channel, wherein the corresponding flow channel communicates the corresponding chamber in the to-be-heated element; the to-be-heated element may further include a heating cavity, The heating cavity can accommodate the above-mentioned heating device, and can be adapted to the heat-conducting sleeve; when the above-mentioned heating device is inserted into the heating cavity, the side wall of the heating cavity can be compatible with the heat-conducting sleeve. Fitted to each other, the heating time of the heating chamber is short, and the heating efficiency of the heating element is improved.

In some optional embodiments, the molecular diagnostic platform of the present application may include a clamping mechanism, and the clamping mechanism can clamp the test tube rack 23 on the cassette 2 . As shown in FIG. 20 and FIG. 21 , the test tube rack 23 The top end may have a protrusion 1101, and its bottom end may have a groove 1102; as shown in FIG. 20, the clamping mechanism may include a clamping jaw 1104 and a supporting plate 1106; the supporting plate 1106 may be located in the clamping jaw 1104 and a preset distance from the clamping jaw 1104, so that a clamping space 1107 is formed between the clamping jaw 1104 and the support plate 1106; optionally, the clamping end of the clamping jaw 1104 A clamping groove 1108 can be opened along its length direction. Preferably, the length of the clamping groove 1108 is equal to the length of the protrusion 1101; the support plate 1106 can be provided with a limiting protrusion 1105, and the limiting protrusion 1105 and The positions of the grooves 1102 correspond.

In the actual use process, when the sample processing module (sample processing module is the prior art, refers to a series of operations from sample pretreatment to nucleic acid purification, to PCR reaction liquid filling, etc., do not do too much here. Explanation, those skilled in the art can understand) inject the PCR reaction body into the capillary, then cover the capillary rack with the end cap 1103 to seal the test tube rack 23, and finally transfer the sealed test tube rack 23 to the thermal cycler module The subsequent PCR process is completed; in this embodiment, the capillary rack can be clamped by the clamping mechanism. Specifically, when the test tube rack 23 in the cassette 2 moves to the preset position, the test tube rack 23 In the clamping space 1107, when the protrusion 1101 is inserted into the clamping groove 1108, and the limiting protrusion 1105 is clamped in the groove 1102, the clamping groove 1108 is used for the protrusion 1101. Therefore, the clamping mechanism can hold the test tube rack 23 stably. The technical problem of low work efficiency and contamination of the test tube rack 23 caused by manually holding the test tube rack 23 in the prior art is overcome.

In conclusion, using the clamping mechanism to realize the clamping effect on the test tube rack 23 replaces the manual operation, which improves the work efficiency on the one hand, and ensures that the capillary is not polluted, so that the result is more accurate.

In this embodiment, the support plate 1106 may be formed of spring steel, and the support plate 1106 formed of the spring steel material has a certain elastic force, which can generate a clamping force when the clamping jaws 1104 cooperate, on the one hand, it does not affect the The test tube rack 23 enters the limiting space, on the other hand, it can ensure that the limiting protrusion 1105 is clamped in the groove 1102 .

Another embodiment of the present application will be described below. This other embodiment is an improvement on the basis of the above-mentioned embodiment. The technical content disclosed in the above-mentioned embodiment will not be described repeatedly, and the content disclosed in the above-mentioned embodiment also belongs to this other embodiment. Example disclosure.

Considering that the test tube rack 23 is provided with a plurality of capillaries at intervals, that is to say, the width of the test tube rack 23 is affected by the number of capillaries. If the test tube rack 23 is too wide, in order to ensure the stable clamping of the test tube rack 23 by the clamping mechanism, in the In this embodiment, the number of the clamping jaws 1104 and the supporting plate 1106 can be set in multiples; the clamping jaws 1104 can be in one-to-one correspondence with the supporting plates 1106 , that is, using a plurality of clamping jaws 1104 The clamping jaws 1104 and the plurality of supporting plates 1106 realize stable clamping of the test tube rack 23 with multi-point positioning.

Specifically, a plurality of the clamping jaws 1104 and the plurality of the supporting plates 1106 are arranged at intervals along a first direction 1109 , and the first direction 1109 refers to the width extending direction of the test tube rack 23 .

Preferably, the number of the clamping jaws 1104 and the supporting plate 1106 is two respectively, which are the first clamping jaw 110, the first supporting plate, the second clamping jaw 111 and the second supporting plate, respectively. The same example applies.

Another embodiment of the present application will be described below. This further embodiment is an improvement on the basis of the above-mentioned embodiment. The technical content disclosed in the above-mentioned embodiment will not be described repeatedly, and the content disclosed in the above-mentioned embodiment also belongs to this further embodiment. Example disclosure.

In the actual experiment process, after the test tube rack 23 is clamped by the clamping mechanism, it is necessary to cover the test tube rack 23 with the end cap 1103, so as to realize the sealing of the test tube rack 23; During the capping operation of the end cap 1103 of the test tube rack 23, it is necessary to drive the clamping mechanism holding the test tube rack 23 to move in the second direction 1118 (moving close to the end cap 103), so as to realize the capping operation of the end cap 1103 of the test tube rack 23.

Based on the above problems, in this further embodiment, the clamping mechanism may further include a first drive assembly, and the first drive assembly may include a fixed cylinder 1115, a first drive member 1116 and a lead screw 1117, preferably, The first driving member 1116 can be a first motor, the output shaft of the first motor is connected with the lead screw 1117 , the fixing cylinder 1115 is provided with a threaded hole, and the fixing cylinder 1115 is sleeved on the lead screw 1117 , and is threadedly connected with the lead screw 1117; the fixing cylinder 1115 is provided with a first clamping jaw 110 and a second clamping jaw 111 at intervals along the first direction 1109, and a first supporting plate is arranged below the first clamping jaw 110 , a second supporting plate is arranged below the second clamping jaw 111 .

Specifically, the first motor can drive the fixing cylinder 1115 to move in the second direction 1118 through the lead screw 1117, so that the first clamping jaw 110 and the first support plate 1106 can move along the second direction 1118. Two directions 1118 move, preferably, the second direction 1118 refers to the vertical upward or vertical downward direction in the normal working state of the clamping mechanism.

More specifically, the end cap 1103 may be located on one side of the test tube rack 23, and the height of the end cap 1103 is smaller than the height of the test tube rack 23. In the actual end cap 1103 capping operation, the clamping mechanism first clamps the test tube rack 23; Then the first motor drives the fixing cylinder 1115 to move vertically downward, so that the groove 1102 at the bottom of the test tube rack 23 is inserted into the protrusion 1101 at the bottom of the end cap 1103, and finally the end cap 1103 of the test tube rack 23 is covered.

To sum up, by using the clamping mechanism with the first drive assembly, on the one hand, the test tube rack 23 can be clamped; set operation.

A further embodiment of the present application will be described below. This further embodiment is an improvement on the basis of the above-mentioned embodiment. The technical content disclosed in the above-mentioned embodiment will not be described repeatedly, and the content disclosed in the above-mentioned embodiment also belongs to this further embodiment. Disclosure of an embodiment.

Based on the problems described in the foregoing embodiments, and considering the stable fixation of the first drive assembly, another structure of the first drive assembly is proposed in this embodiment. The first drive assembly may include a fixed cylinder 1115, a first drive member 1116, a first support frame 1112, and a second support frame 1113 disposed inside the first support frame 1112. Preferably, the first drive member 1116 may be The first motor; the first drive member 1116 can be disposed on the top of the first support frame 1112, the two ends of the fixing cylinder 1115 are fixed on the second support frame 1113, the first drive member 1116 The output shaft is connected with the second support frame 1113 through the lead screw 1117, that is, when the first motor is driven, the second support frame 1113 can be driven to move in the second direction 1118 by the lead screw 1117. When the second direction 1118 moves, the fixing cylinder 1115 can cooperate with the clamping jaws 1104 and the support plate 1106 to move in the second direction 1118 , thereby realizing the covering of the end caps 1103 of the test tube rack 23 .

In order to ensure that the second support frame 1113 can run stably on the first support frame 1112, in this further embodiment, the clamping mechanism may further include an auxiliary member 1125, and the auxiliary member 1125 may include a slideway 1126 and a sliding A slider 1127 placed on the slideway 1126; the slideway 1126 can be set on the end surface of the first support frame 1112 facing the second support frame 1113, and the slide block 1127 can be set on the The second support frame 1113 faces the end surface of the first support frame 1112 .

Specifically, with the aid of the auxiliary member 1125 , the second support frame 1113 can not only achieve stable sliding on the first support frame 1112 , but also give the second support frame 1113 a certain guiding effect.

Another embodiment of the present application will be described below. The other embodiment is an improvement on the basis of the above-mentioned embodiment. The technical content disclosed in the above-mentioned embodiment will not be described repeatedly, and the content disclosed in the above-mentioned embodiment also belongs to this other embodiment. Examples of disclosures.

22 to 25, when the test tube rack 23 is clamped and the end cap 1103 is covered, the fixing cylinder 1115 will be subjected to a large force in the vertical direction. Once the self-locking force of the first motor is insufficient In order to support this force, the fixed cylinder 1115 will have the problem of turning and tilting. In order to overcome such problems, the clamping mechanism further includes a limiting member; when the clamping jaws 1104 and the support plate 1106 clamp the When the piece is to be clamped, the limiting member can prevent the fixing cylinder 1115 from rotating.

Specifically, the limiting member may be a limiting pin 1129; the limiting pin 1129 may be disposed below the fixing cylinder 1115, and a limiting position is provided on the fixing cylinder 1115 corresponding to the limiting pin 1129 hole.

During actual use, when the first driving component drives the fixing cylinder 1115 to move along the second direction 1118 , the limiting pin 1129 can be inserted into or pulled out of the limiting hole.

When the first motor drives the fixing cylinder 1115 to move down a certain distance along the second direction 1118, the limiting pin 1129 can rotate into the limiting hole, and the fixing cylinder 1115 is limited at this time; After completing the covering action of the end cap 1103, when moving up along the second direction 1118, the limiting pin 1129 leaves the limiting hole.

In other embodiments, after the end caps 1103 are capped on the test tube racks 23, the test tube racks 23 need to be transferred to a PCR thermal cycler module (PCR thermal cycler module is the core technology of rapid molecular detection) to achieve For the thermal cycle of the test tube rack 23, in order to facilitate the transfer of the test tube rack 23 to the PCR thermal cycle module, the molecular diagnostic platform of the present application further includes a transfer device, and the transfer device includes a second drive assembly and the above-mentioned clamping mechanism; the second The output shaft of the driving assembly is connected with the fixing cylinder 1115, and the second driving member 1131 can drive the fixing cylinder 1115 to rotate by a preset angle so that the clamping mechanism transfers the to-be-clamped piece from the first position to the second position.

To sum up, the transfer device can realize the transfer of the test tube racks 23 , which overcomes the manual hand-held transfer method in the prior art. On the one hand, the work efficiency is improved; on the other hand, the test tube racks 23 are not polluted.

Considering that the fixing cylinder 1115 is fixed on the second support frame 1113, the space on the upper part of the fixing cylinder 1115 is limited, preferably, the fixing cylinder 1115 can rotate counterclockwise during the rotation process, that is, the second driving member 1131 drives the fixing cylinder 1115 to rotate. The barrel 1115 rotates in a counterclockwise direction.

Specifically, the first position refers to the position of the test tube rack 23 in the cassette 2 on one side of the second support frame 1113 , and the second position refers to the position on the other side of the second support frame 1113 The position of the supporting portion for supporting the test tube rack 23.

Preferably, the preset angle may be 180°.

In this embodiment, the second driving assembly may include a second driving member 1131; the second driving member 1131 may be disposed on the second supporting frame 1113, and the output shaft of the second driving member 1131 may be Passing through the second support frame 1113 and inserted into the interior of the fixing cylinder 1115; preferably, the second driving member 1131 may be a second motor.

Specifically, when the second motor drives the output shaft to rotate by the predetermined angle, the fixed cylinder 1115 can be rotated by the predetermined angle.

Considering that when the second motor works, it will generate vibration, in order to prevent this vibration from being transmitted to the test tube rack 23 and affecting the test tube rack 23, in this embodiment, the transfer member may further include a buffer 1132, so One end of the buffer member 1132 can abut on the first support frame 1112, and the other end can abut on the second driving member 1131; preferably, the buffer member 1132 can be a spring, which is activated by a spring to shock absorption.

Besides, the present application may also be provided with a support platform 1133 , and the clamping mechanism may be provided on the support platform 1133 .

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, but not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: The technical solutions described in the foregoing embodiments can still be modified, or some or all of the technical features thereof can be equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the embodiments of the present application. scope.

Industrial Applicability

According to the molecular diagnostic platform provided by the present application, the operator can place the cassette on the cassette moving assembly, and then transport the cassette to the workstation of the sample processing module through the cassette moving assembly; the sample processing module can be configured to be used for Operate the cartridge to make the reagents in the cartridge react with the sample in a predetermined order to complete the nucleic acid extraction reaction, and fill the obtained sample solution into the capillary; the sample transfer module is located between the sample processing module and the thermal cycle module. After the nucleic acid extraction of the sample is completed in the cartridge and filled in the capillary, the test tube rack is transferred to the thermal cycler module through the sample transfer module, so that the sample is subjected to PCR reaction at the thermal cycler module, and the capillary tube that has completed the PCR reaction is passed through the optical module. Fluorescence quantitative detection is performed on the samples inside to collect fluorescence signals and obtain fluorescence imaging photos. Therefore, in the whole detection process, the operator only needs to place the cassette containing the samples and various reagents on the rack, and the subsequent operations can be completed automatically and efficiently through each module.

Furthermore, it will be appreciated that the racks, sample processing modules, sample transfer modules, etc. of the present application are reproducible and can be used in a variety of industrial applications. For example, the rack of the present application can be applied to a molecular diagnostic platform that needs to realize fluorescence quantitative detection.

Claims (15)

1. A molecular diagnostic platform is characterized by comprising a rack, a cassette, a sample processing module, a sample transfer module, a thermal cycle module and an optical module;

   the sample processing module, the sample transfer module, the thermal cycle module and the optical module are respectively arranged on the rack;

   The cartridge is provided with a test tube rack on which capillaries are installed; the sample processing module is configured to operate the cartridge, so that the sample in the cartridge completes the nucleic acid extraction reaction, and the The obtained sample solution is filled in the capillary;

   the sample transfer module is configured to transfer the test tube rack between the sample processing module and the thermal cycling module;

   the thermal cycling module is configured to thermally cycle the sample within the capillary;

   The optical module includes a light source assembly, an imaging assembly, and a fiber optic bundle; the light source assembly is configured to provide excitation light, and the light source assembly illuminates the capillary tube located at the thermal cycling module through the fiber optic bundle, to excite fluorescence; the fluorescence is irradiated through the fiber optic bundle into the imaging assembly configured to collect the fluorescence and form a fluorescence imaging picture.
2. The molecular diagnostic platform according to claim 1, wherein a cassette moving assembly is arranged on the rack;

   The cartridge is placeable on the cartridge moving assembly configured to move the cartridge to the sample processing module.
3. The molecular diagnostic platform according to claim 1 or 2, wherein the cassette comprises a cassette body and a pipetting mechanism;

   The cartridge body is formed with a plurality of compartments, the test tube rack is connected with the cartridge body, and a plurality of the capillary tubes are installed on the test tube rack;

   The pipetting mechanism is connected with the cartridge body, and the sample processing module can act on the pipetting mechanism, so that the pipetting mechanism transfers reagents between the plurality of chambers and the capillary.
4. The molecular diagnostic platform according to claim 3, wherein the plurality of compartments comprises a sample compartment for storing samples to be tested, a waste fluid compartment for storing waste liquid, and a reconstitution compartment for storing reconstituted solution , the bottom of the cartridge has a plurality of flow channels, and the plurality of flow channels can conduct the corresponding chambers in the plurality of chambers.
5. The molecular diagnostic platform according to claim 3 or 4, wherein the pipetting mechanism comprises a main mechanical valve and an auxiliary mechanical valve;

   The main mechanical valve and the auxiliary mechanical valve are respectively rotatably connected to the main body of the cartridge, and both the main mechanical valve and the auxiliary mechanical valve include a valve body and a valve stem;

   The valve body of the main mechanical valve can be rotated, so that the valve body of the main mechanical valve is communicated with a plurality of the chambers respectively; Reagent transfer between the valve body of the valve and said compartment in communication with it;

   The valve body of the auxiliary mechanical valve can be rotated, so that the valve body of the auxiliary mechanical valve is respectively communicated with a plurality of capillaries on the test tube rack and a plurality of reconstitution chambers in the chambers; the auxiliary machinery The valve stem of the valve can perform piston movement, so that the auxiliary mechanical valve can extract the sample solution in the reconstitution chamber and fill the sample solution into the plurality of capillaries.
6. The molecular diagnostic platform according to claim 4, wherein the sample processing module comprises a cassette operation unit;

   The cartridge operation unit includes a valve body rotating assembly, a main valve stem lifting assembly and a secondary valve stem lifting assembly;

   The valve body rotating assembly can be connected with the valve bodies of the main mechanical valve and the auxiliary mechanical valve to drive the valve bodies of the main mechanical valve and the auxiliary mechanical valve to rotate;

   The main valve stem lifting assembly can be connected with the valve stem of the main mechanical valve, so as to drive the valve stem of the main mechanical valve to perform piston movement in the valve body of the main mechanical valve;

   The auxiliary valve stem lifting assembly can be connected with the valve stem of the auxiliary mechanical valve, so as to drive the valve stem of the auxiliary mechanical valve to perform piston movement in the valve body of the auxiliary mechanical valve.
7. The molecular diagnostic platform according to any one of claims 3 to 6, wherein the sample processing module further comprises a physical processing unit;

   The physical processing unit is arranged below the main body of the cartridge, and the physical processing unit includes a lift drive device, a magnetic attraction component and an ultrasonic heating component;

   The magnetic attraction assembly and the ultrasonic heating assembly are respectively connected with the drive ends of the lift drive device;

   The lift driving device is configured to drive the magnetic attraction assembly and the ultrasonic heating assembly up and down, so as to make the magnetic attraction assembly or the ultrasonic heating assembly and the samples in the plurality of chambers of the cartridge against the outer walls of the tank;

   the ultrasonic heating assembly is configured to heat and sonicate the sample in the sample chamber;

   The magnetic attraction assembly is configured to attract magnetic beads in the sample chamber.
8. The molecular diagnostic platform according to claim 7, wherein the physical processing unit further comprises a translation drive device;

   The lift drive device is disposed on the translation drive device, and the translation drive device can drive the lift drive device to move, so as to move the magnetic attraction component or the ultrasonic heating component below the sample chamber.
9. The molecular diagnostic platform of any one of claims 1 to 8, wherein the sample transfer module is located between the sample processing module and the thermal cycling module, and wherein the sample transfer module includes a clamp Claw mechanism and jaw lifting device;

   The clamping jaw mechanism is mounted on the clamping jaw lifting device, and the clamping jaw lifting device is configured to drive the clamping jaw mechanism to rise and fall;

   The gripper mechanism can grab the test tube rack, and the gripper mechanism can rotate to drive the test tube rack to turn over a predetermined angle, and turn the test tube rack from the sample processing module to the thermal cycler at the module.
10. The molecular diagnostic platform according to any one of claims 1 to 9, wherein the thermal cycle module comprises a moving mechanism, a reverse transcription tank, a high temperature tank, a low temperature tank and a photodetection tank;

    The reverse transcription tank, the high temperature tank, the low temperature tank and the photodetection tank are arranged side by side at intervals, and the reverse transcription tank, the high temperature tank, the low temperature tank and the photodetection tank are respectively provided There are temperature control elements;

    The sample transfer module can place the test tube rack on the moving mechanism, so as to move the test tube rack to the reverse transcription tank, the high temperature tank, the low temperature tank or the low temperature tank through the moving mechanism above the optical detection tank, and place the capillary tube on the test tube rack in the reverse transcription tank, the high temperature tank, the low temperature tank or the optical detection tank.
11. The molecular diagnostic platform according to claim 10, wherein the moving mechanism comprises a first sliding mechanism, a second sliding mechanism and a supporting plate, the supporting plate is slidably connected to the second sliding mechanism, and passes through the The second sliding mechanism is slidably connected with the first sliding mechanism, so that the first sliding mechanism drives the support plate to move from the end close to the sample transfer module to the reverse transcription tank and the high temperature tank in sequence. , above the cryogenic tank or the optical detection tank; and the second sliding mechanism is configured to drive the support plate to rise and fall.
12. The molecular diagnostic platform according to claim 10 or 11, wherein the photodetection groove is formed with a plurality of photodetection cavities, and a plurality of the photodetection chambers are in one-to-one correspondence with a plurality of the capillaries;

    A plurality of light-passing holes are opened at positions opposite to the plurality of light-checking cavities on the sidewall of the light-detecting slot, and the plurality of light-passing holes are respectively communicated with the corresponding light-checking cavities, so as to enable excitation Light can be irradiated to the capillary.
13. The molecular diagnostic platform according to claim 12, wherein the optical detection slot is further provided with an optical fiber fixing plate;

    The optical fiber fixing plate abuts against the side wall of the optical detection slot on which the light-passing hole is formed, and the optical fiber fixing plate is provided with a plurality of communication holes, and the plurality of communication holes are connected with the plurality of communication holes. The light-passing holes are in one-to-one correspondence and communicated;

    The communication holes are used to connect the optical fiber bundles, and a focusing lens is clamped and installed between each of the communication holes and the corresponding light-passing holes.
14. The molecular diagnostic platform according to claim 12, wherein a heating plate is provided on the bottom wall of the photodetection tank for heating the photodetection tank, and the photodetection tank is formed with a first A heat preservation chamber and a second heat preservation chamber, the plurality of photodetection chambers may be located between the first heat preservation chamber and the second heat preservation chamber, and a heat preservation layer is provided on the outside of the photodetection tank to The photodetection cell is kept warm.
15. The molecular diagnostic platform according to claim 13, wherein the number of the optical fiber bundles is multiple, and the multiple optical fiber bundles are in one-to-one correspondence with the multiple capillaries;

    The multiple optical fiber bundles each include three ports, the first ports of the multiple optical fiber bundles are all connected to the light source assembly, and the second ports of the multiple optical fiber bundles are respectively connected to the multiple communication holes. , the third ports of the plurality of optical fiber bundles are all connected to the imaging assembly;

    The excitation light emitted by the light source assembly can enter the first ports of the plurality of optical fiber bundles, and is irradiated onto the corresponding capillary tubes through the second ports of the plurality of optical fiber bundles, so as to excite the interior of the plurality of the capillary tubes. The fluorescent substance emits fluorescence;

    The fluorescent light can enter the second ports of the plurality of fiber optic bundles and illuminate the imaging assembly through the third ports of the fiber optic bundles.

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