CN106222068A - Miniature PCR system of capillary glass tube and preparation method thereof - Google Patents
Miniature PCR system of capillary glass tube and preparation method thereof Download PDFInfo
- Publication number
- CN106222068A CN106222068A CN201610705502.3A CN201610705502A CN106222068A CN 106222068 A CN106222068 A CN 106222068A CN 201610705502 A CN201610705502 A CN 201610705502A CN 106222068 A CN106222068 A CN 106222068A
- Authority
- CN
- China
- Prior art keywords
- glass tube
- module
- capillary glass
- pcr
- chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
Abstract
Open a kind of miniature PCR system of capillary glass tube of the present invention and preparation method thereof, described PCR system includes chip carrier, pcr chip, fan, wherein: pcr chip is made up of capillary glass tube, base plate, the inside of capillary glass tube is PCR reaction chamber, lower surface at base plate is provided with and adds thermode, the upper surface of base plate is provided with thermometric electrode and deep trouth, and described capillary glass tube is embedded in deep trouth, is filled with heat-conducting resin in deep trouth.The pcr chip reaction chamber of the present invention is capillary glass tube, can be used for the pcr chip realizing rapid temperature rise and drop, temperature homogeneity is good, amplification efficiency is high.Consumptive material in the miniature pcr chip of capillary glass tube of the present invention is capillary glass tube, make have add thermode, thermometric electrode and the base plate of deep trouth and on can repeatedly use, while this avoids cross-contamination, it is greatly reduced the use cost of chip.
Description
Technical field
The present invention relates to polymerase chain reaction (PCR) system, in particular it relates to a kind of capillary glass tube miniature PCR system
System and its preparation method thereof.
Background technology
Polymerase chain reaction (PCR) is a kind of Protocols in Molecular Biology for amplifying amplification specific DNA fragments, is raw
Special DNA replication dna outside object, the DNA of trace can be significantly increased by it.By nineteen eighty-three American scientist K.B.Mullis first
Advance an idea, within 1985, invented polymerase chain reaction by it, it means that the real birth of round pcr.Through more than 30 years
Development, round pcr has been widely used in health analysis and has divided with medical diagnosis on disease, agricultural, ecological environment monitoring, criminal case
The fields such as analysis.
Pcr chip based on MEMS microflow control technique is by traditional PCR reaction unit microminaturization, thus substantially reduces
The volume of device, power consumption, price, decrease the consumption of reaction reagent, speeded response speed.Currently, pcr chip has following
Feature: 1, use polymer (such as: PDMS, SU8, PC etc.) material, utilize the thermoplasticity of polymer or the mobility of monomer whose, pressure
Mould or injection molding make fluid chamber；2, the chamber that pressing mold or injection molding make is planar flat structure, and surface-to-volume ratio is big；
3, most of chips all will add thermode or temperature sensing electrode integrates with fluid chamber, in order to avoid cross-contamination,
Chip is single use.Existing pcr chip advantage is the most prominent, but there is also a lot of not enough place, such as: 1, polymeric material
Being generally of the strongest protein adsorption, in order to improve the reaction efficiency of pcr chip, chip chamber needs to carry out surface process,
This makes complex manufacturing technology；2, pressing mold or the molding process process of polymer are complicated, processing cost is of a relatively high；3, use
Flat two-dimentional chamber structure, is easily generated bubble at edge；4, cost of manufacture is of a relatively high.
Through retrieval, the Chinese invention application of Publication No. 102899238A, this disclosure of the invention a kind of continuous stream PCR and hair
The micro flow control chip device that cons electrophoresis function is integrated, including continuous-flow PCR chip and capillary electrophoresis chip, its feature
It is that the output channel of described continuous-flow PCR chip is directly connected in the sample cell of capillary electrophoresis chip, described capillary
Electrophoresis tube chip is provided with sample intake passage and split tunnel, and described sample intake passage and split tunnel are decussation, described sample introduction
The top of passage is sample cell and sample waste pond, and the top of described split tunnel is buffer pool and waste liquid pool.
But the integrated microfluidic chip of above-mentioned patent disclosure is a kind of not reproducible use device, causes cost relatively
High.
Summary of the invention
For deficiency of the prior art, it is an object of the invention to provide a kind of miniature PCR system of capillary glass tube and
Preparation method, employing add thermode, thermometric electrode separates with PCR reaction chamber, adds thermode, thermometric electrode is repeatable makes
With, this greatly reduces cost of manufacture.
For realizing object above, the present invention by the following technical solutions:
According to the first aspect of the invention, it is provided that a kind of miniature PCR system of capillary glass tube, including chip carrier, PCR core
Sheet, fan, wherein:
Described pcr chip is made up of capillary glass tube, base plate, and the inside of capillary glass tube is PCR reaction chamber, at base plate
Lower surface be provided with and add thermode, the upper surface of base plate is provided with thermometric electrode and deep trouth, and described capillary glass tube is embedded in deep trouth
In, in deep trouth, it is filled with heat-conducting resin；
Described pcr chip is fixed on chip carrier by support beam, and the chip carrier immediately below pcr chip makes to be had
Air holes, is provided with fan, rotates, after fan energising, the flowing making air in the bottom of chip carrier, and PCR core is easy in the flowing of air
Heat Convention diffusion on sheet, reaches the effect of cooling.
Preferably, add thermode, employing platinum or aluminum or copper or gold metallic film on described base plate make, and to metal foil
Film is patterned so that the temperature of capillary glass tube region tends to uniform.
Preferably, described pcr chip connects has telemetry circuit, described telemetry circuit to include power module, temperature measurement module, add
Thermal drivers module and cooling drive module, wherein:
The photovoltaic conversion that outside inputs is become control circuit each module required voltage value by power module；
Temperature measurement module passes through constant-current source circuit, and the resistance value equal proportion of thermometric electrode on pcr chip is converted into magnitude of voltage,
Read for subsequent data acquisition system and heating and cooling control program and judge；
Heat driven module, for adding thermocurrent to the thermode output that adds of pcr chip, the thermal resistance effect adding thermocurrent makes
The temperature obtaining capillary glass tube region rises；
Cooling drives module, controls startup and the disconnection of fan, for the region by the capillary glass tube place of pcr chip
Temperature lowers.
Preferably, described pcr chip connects data acquisition and procedure control unit, described data acquisition and programme-control
Unit includes gathering and control module and central controller module, gather with the temperature measurement module on control module and telemetry circuit,
Heat driven module, cooling drive module to be connected；Wherein:
Gather, with control module, the analog output voltage of temperature measurement module is changed into digital signal, be transferred to central authorities' control simultaneously
Device module processed；
Central controller module is by controlling program, it is judged that the Current Temperatures of capillary glass tube region and target temperature
Between error, if Current Temperatures is higher than range of error, then sends instruction with control module to gathering, start cooling and drive module
And cooling fan, the regional temperature at capillary glass tube place is lowered；If Current Temperatures is less than range of error, then to gather with
Control module sends instruction, starts heat driven module, and heat driven module adds thermoelectricity to the thermode output that adds of pcr chip
Stream, the thermal resistance effect adding thermocurrent makes the temperature of capillary glass tube region rise.
Present system uses PCR reaction chamber with electrode separation formula structure, surface-to-volume than little PCR reaction chamber
Three-dimension curved surface, non-polymer material make, and simple in construction, heating and cooling are rapid, this structure is good to the compatibility of biomacromolecule,
Protein adsorption extremely pettiness, processing cost is low.
According to a further aspect in the invention, it is provided that the preparation method of the miniature PCR system of a kind of capillary glass tube, including as follows
Step:
Using the substrate identical with baseboard material, the upper surface at substrate carries out deposit metal films, uses photoetching process,
Patterned photoresist defines the figure of metallic film, then uses dry etching or wet-etching technology to remove and comes out
Metallic film, it is thus achieved that the thermometric electrode of upper surface；
Lower surface at substrate deposits metallic film, uses the figure of double-sided alignment photoetching process definition lower surface metal thin film
Shape, uses dry etching or wet-etching technology to remove the metallic film come out, it is thus achieved that to add thermode, the thermometric of upper surface
Electrode is strictly directed at the relative position added between thermode of lower surface；
Between the thermometric electrode of upper surface, machine cuts processing technique is used to make the deep of capillary glass tube embedding
Groove；
Finally each unit is cut down, it is thus achieved that single pcr chip.
Compared with prior art, the present invention has a following beneficial effect:
The pcr chip reaction chamber of the present invention is capillary glass tube, and capillary glass tube has low cost, inhales without albumen
Attached, the generation of good hydrophilic property, bubble-free, good airproof performance, the advantage such as planar dimension is little, surface-to-volume ratio is little, can be used for realizing
The pcr chip that rapid temperature rise and drop, temperature homogeneity are good, amplification efficiency is high.In the miniature pcr chip of capillary glass tube of the present invention
Consumptive material is capillary glass tube, make have add thermode, thermometric electrode and the base plate of deep trouth and on can repeatedly use,
While this avoids cross-contamination, it is greatly reduced the use cost of chip.
Deep trouth on the pcr chip base plate of the present invention uses the mode of machining to make, and technique is simple, processing cost
Low, the most also can make big aspect ratio structures.The pcr chip structure of the present invention uses the base plate of small volume, this reduces core
The thermal capacity of sheet so that warming and cooling rate speeds；Meanwhile, by using linear contact lay to support, reduce pcr chip and chip carrier it
Between contact area, decrease due to contact bring heat conduction, this be conducive to accelerate chip heating rate, improve PCR
The work efficiency of chip and temperature homogeneity.
Accompanying drawing explanation
By the detailed description non-limiting example made with reference to the following drawings of reading, the further feature of the present invention,
Purpose and advantage will become more apparent upon:
Fig. 1 is the miniature PCR system general three view of the capillary glass tube in one embodiment of the invention；
Fig. 2 is the miniature PCR system general three broken away view of the capillary glass tube in one embodiment of the invention；
Fig. 3 is the chip carrier three-dimensional broken away view in one embodiment of the invention；
Fig. 4 is the oblique upper 3-D view of the pcr chip in one embodiment of the invention；
Fig. 5 is the oblique upper 3-D view of the pcr chip in one embodiment of the invention；
Fig. 6 is that the miniature PCR system of the capillary glass tube in one embodiment of the invention controls logic diagram；
Fig. 7 is the pcr chip micro Process preparation flow figure in one embodiment of the invention；
In figure: support beam 1,2；Pcr chip 3；Thermometric electrode 4,6；Capillary glass tube 5；Chip carrier 7；Chip carrier leg 8；
Fan 9；Support beam embedded groove 10,11；Air outlet 12；Fan support face 13；Air intake vent 14；Thermometric contact conductor foot 41,42；The end
Plate 43；Deep trouth 44；Add thermode 45；Heating contact conductor foot 46.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following example will assist in the technology of this area
Personnel are further appreciated by the present invention, but limit the present invention the most in any form.It should be pointed out that, the ordinary skill to this area
For personnel, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement.These broadly fall into the present invention
Protection domain.
As shown in Figure 1, Figure 2, Figure 3, Figure 4, the embodiment of the miniature PCR system of a kind of capillary glass tube, including support beam 1,
2；Pcr chip 3；Thermometric electrode 4,6；Capillary glass tube 5；Chip carrier 7；Chip carrier leg 8；Fan 9.
Described pcr chip 3 is made up of capillary glass tube 5, base plate 43, and the inside of capillary glass tube 5 is PCR reaction chamber,
Lower surface at base plate 43 is provided with and adds thermode 45, and the upper surface of base plate 43 is provided with thermometric electrode 4,6 and deep trouth 44, described glass
Capillary tube 5 is embedded in deep trouth 44, is filled with heat-conducting resin in deep trouth 44；
Described pcr chip 3 is fixed on chip carrier 7 by support beam 1,2, and support beam 1,2 is embedded in the upper table of chip carrier 7
The support beam embedded groove 10,11 in face.Chip carrier 7 is immediately below pcr chip 3.
Be provided with fan 9 in the bottom of chip carrier 7, fan 9 is positioned by the fan support face 13 within chip carrier 7, and core
Bar 7 forms a fixed connection, and chip carrier leg 8 has four, is positioned on four angles bottom chip 7, for one of chip carrier 7
Ingredient.
As one preferably, as it is shown on figure 3, the upper surface of chip carrier 7 makes support beam embedded groove 10,11, support beam
The height of the depth ratio support beam 1,2 of embedded groove 10,11 is little.Exhaust vent 12 is arranged at the top of chip carrier 7, facilitates fan 9 to blow out
Air flows out.Air intake vent 14 is arranged at the bottom of chip carrier 7, facilitates the inflow of air.After fan 9 energising, PCR is easy in the flowing of air
Heat Convention diffusion on chip 3, reaches the effect of cooling.
As one preferably, as shown in Figure 4, Figure 5, the both sides of pcr chip 3 upper surface deep trouth 44 be distributed thermometric electrode 4,
6.The signal of telecommunication is introduced and extraction by thermometric electrode 4,6 by thermometric contact conductor foot 41,42.Thermometric electrode 4,6 is metallic film
Structure, the function that resistance value is temperature of thermometric electrode 4,6.Pcr chip 3 lower surface makes to have and adds thermode 45, by heating
Contact conductor foot 46 is passed through and adds thermocurrent, utilizes the electrocaloric effect adding thermocurrent to make pcr chip 3 temperature raise.
As one preferably, the outer telemetry circuit that is with of described pcr chip 3, telemetry circuit by power module, temperature measurement module,
Heat driven module, cooling drive module.The photovoltaic conversion that outside inputs is become electricity needed for each module of control circuit by power module
Pressure value.Temperature measurement module passes through constant-current source circuit, and the resistance value equal proportion of thermometric electrode on pcr chip 34,6 is converted into voltage
Value, judges with procedure control unit for subsequent data acquisition.The core component of heat driven module is a power amplification circuit,
When data acquisition sends heating instructions with procedure control unit to telemetry circuit, heat driven module starts, and to pcr chip
The thermode 45 that adds on 3 is passed through and adds thermocurrent, and the thermal resistance effect adding thermode 45 produces heat so that capillary glass tube 5 place
The temperature in region raises, and reaches preset value.
Described pcr chip 3 connects data acquisition and procedure control unit.Described data acquisition and procedure control unit bag
Include collection and control module 16 and central controller module, gather with control module 16 and the temperature measurement module on telemetry circuit, add
Thermal drivers module, cooling drive module to be connected.Gather, with control module 16, the analog output voltage of temperature measurement module is changed into number
Word signal, is transferred to central controller module simultaneously.Central controller module is by controlling program, it is judged that capillary glass tube 5 institute
Error between the Current Temperatures and target temperature in region.If Current Temperatures is higher than range of error, then to gathering and controlling mould
Block 16 sends instruction, starts cooling and drives module and cooling fan, is lowered by the regional temperature at capillary glass tube 5 place.If
Current Temperatures is less than range of error, then to gathering and control module 16 transmission instruction, start heat driven module, heat driven mould
Block adds thermocurrent to thermode 45 output that adds of pcr chip 3, and the thermal resistance effect adding thermocurrent makes capillary glass tube 5 location
The temperature in territory rises.
As shown in Figure 6, for the temperature control logic block diagram of the miniature PCR system of capillary glass tube, whole control process makes
The PCR reaction chamber temperature of capillary glass tube 5 reaches target temperature as early as possible.The temperature measurement module of telemetry circuit 15 by thermometric electrode 4,
The resistance of 6 approximately linearly changes into magnitude of voltage, samples with control module 16 for data acquisition.Data acquisition and control module 16
By photovoltaic conversion become digital quantity, be then passed to host computer 17 i.e. central controller module, host computer 17 runs program, compares glass
The Current Temperatures of glass capillary tube 5 and target temperature value size set in advance, if Current Temperatures is higher than range of error, then send
Control signal is to telemetry circuit 15, and the cooling of telemetry circuit 15 drives module to start fan 9 so that around capillary glass tube 5
Air flows, and takes away heat so that capillary glass tube temperature 5 declines.If Current Temperatures is less than range of error, then host computer 17
Sending control signal to telemetry circuit 15, the heat driven module of telemetry circuit 15 sends and adds thermocurrent to adding thermode 45, adds
The Joule heat of thermode 45 makes the temperature of capillary glass tube 5 rise.
As it is shown in fig. 7, be the microfabrication preparation flow of pcr chip, specifically include:
Using the substrate identical with base plate 43 material, the upper surface at substrate carries out deposit metal films, then uses light
Carving technology, patterned photoresist defines the figure of metallic film, then uses dry etching or wet-etching technology to remove
The metallic film come out, it is thus achieved that thermometric electrode 4,6.
Lower surface at substrate deposits metallic film, uses the figure of double-sided alignment photoetching process definition lower surface metal thin film
Shape, the same metallic film using dry etching or wet-etching technology removal to come out, it is thus achieved that add thermode 45, upper surface
Thermometric electrode 4,6 be strictly directed at the relative position added between thermode 45 of lower surface.
Between upper surface thermometric electrode 4,6, machine cuts processing technique is used to make deep trouth 44.
Finally each unit is cut down, it is thus achieved that single pcr chip 3.
The pcr chip reaction chamber of the present invention is capillary glass tube, and capillary glass tube has low cost, inhales without albumen
Attached, the generation of good hydrophilic property, bubble-free, good airproof performance, the advantage such as planar dimension is little, surface-to-volume ratio is little, can be used for realizing
The pcr chip that rapid temperature rise and drop, temperature homogeneity are good, amplification efficiency is high.
Consumptive material in the miniature pcr chip of capillary glass tube of the present invention is capillary glass tube, makes and has and add thermode, thermometric
Electrode and the base plate of deep trouth and on can repeatedly use, while this avoids cross-contamination, be greatly reduced
The use cost of chip.
Above the tool embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned spy
Determining embodiment, those skilled in the art can make various deformation or amendment within the scope of the claims, and this has no effect on
The flesh and blood of the present invention.
Claims (5)
1. the miniature PCR system of capillary glass tube, it is characterised in that: include chip carrier, pcr chip, fan, wherein:
Described pcr chip is made up of capillary glass tube, base plate, and the inside of capillary glass tube is PCR reaction chamber, under base plate
Surface is provided with and adds thermode, and the upper surface of base plate is provided with thermometric electrode and deep trouth, and described capillary glass tube is embedded in deep trouth,
Heat-conducting resin it is filled with in deep trouth；
Described pcr chip is fixed on chip carrier by support beam, and the chip carrier immediately below pcr chip makes air-out
Hole, is provided with fan, rotates, after fan energising, the flowing making air in the bottom of chip carrier, and pcr chip is easy in the flowing of air
On heat Convention diffusion, reach cooling effect.
The miniature PCR system of capillary glass tube the most according to claim 1, it is characterised in that: add thermoelectricity on described base plate
Pole, uses platinum or aluminum or copper or gold metallic film to make, and is patterned metallic film so that capillary glass tube location
The temperature in territory tends to uniform.
The miniature PCR system of capillary glass tube the most according to claim 1 and 2, it is characterised in that: described pcr chip connects
Power module, temperature measurement module, heat driven module and cooling drive module, wherein to have telemetry circuit, described telemetry circuit to include:
The photovoltaic conversion that outside inputs is become control circuit each module required voltage value by power module；
Temperature measurement module passes through constant-current source circuit, and the resistance value equal proportion of thermometric electrode on pcr chip is converted into magnitude of voltage, after supplying
Continuous data collecting system reads and heating and cooling control program and judge；
Heat driven module, for adding thermocurrent to the thermode output that adds of pcr chip, the thermal resistance effect adding thermocurrent makes glass
The temperature of glass capillary tube region rises；
Cooling drives module, controls startup and the disconnection of fan, for the regional temperature by the capillary glass tube place of pcr chip
Lower.
The miniature PCR system of capillary glass tube the most according to claim 3, it is characterised in that: described pcr chip connects number
According to gathering and procedure control unit, described data acquisition and procedure control unit include gathering and control module and central controller
Module, gathers and drives module to be connected with control module with the temperature measurement module on telemetry circuit, heat driven module, cooling；Wherein:
Gather, with control module, the analog output voltage of temperature measurement module is changed into digital signal, be transferred to central controller simultaneously
Module；
Central controller module is by controlling program, it is judged that between Current Temperatures and the target temperature of capillary glass tube region
Error, if Current Temperatures is higher than range of error, then send instruction with control module to gathering, start cooling and drive module and cold
But fan, lowers the regional temperature at capillary glass tube place；If Current Temperatures is less than range of error, then to gathering and controlling
Module sends instruction, starts heat driven module, and heat driven module adds thermocurrent to the thermode output that adds of pcr chip, adds
The thermal resistance effect of thermocurrent makes the temperature of capillary glass tube region rise.
5. the preparation method of the miniature PCR system of capillary glass tube described in an any one of claim 1-4, it is characterised in that: bag
Include following steps:
Using the substrate identical with baseboard material, the upper surface at substrate carries out deposit metal films, uses photoetching process, figure
The photoresist changed defines the figure of metallic film, then uses dry etching or wet-etching technology to remove the gold come out
Belong to thin film, it is thus achieved that the thermometric electrode of upper surface；
Lower surface at substrate deposits metallic film, uses the figure of double-sided alignment photoetching process definition lower surface metal thin film,
Dry etching or wet-etching technology is used to remove the metallic film come out, it is thus achieved that to add thermode, the thermometric electricity of upper surface
Pole is strictly directed at the relative position added between thermode of lower surface；
Between the thermometric electrode of upper surface, machine cuts processing technique is used to make the deep trouth that capillary glass tube embeds；
Finally each unit is cut down, it is thus achieved that single pcr chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610705502.3A CN106222068A (en) | 2016-08-22 | 2016-08-22 | Miniature PCR system of capillary glass tube and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610705502.3A CN106222068A (en) | 2016-08-22 | 2016-08-22 | Miniature PCR system of capillary glass tube and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106222068A true CN106222068A (en) | 2016-12-14 |
Family
ID=57552835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610705502.3A Pending CN106222068A (en) | 2016-08-22 | 2016-08-22 | Miniature PCR system of capillary glass tube and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106222068A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929409A (en) * | 2017-03-17 | 2017-07-07 | 上海交通大学 | Glass sleeve pipe structure pcr chip array |
| CN108865818A (en) * | 2017-05-08 | 2018-11-23 | 广州康昕瑞基因健康科技有限公司 | sequencing reaction device |
| CN109207645A (en) * | 2018-11-01 | 2019-01-15 | 福建省博凯科技有限公司 | The control method of one-part form thermal convection instrument |
| CN109486652A (en) * | 2018-11-12 | 2019-03-19 | 安徽中医药高等专科学校 | A kind of PCR micro-fluidic chip and preparation method thereof |
| WO2020207295A1 (en) * | 2019-04-09 | 2020-10-15 | 京东方科技集团股份有限公司 | Reaction device and reaction system for detection chip |
| CN113249196A (en) * | 2021-05-06 | 2021-08-13 | 北京谊安和景生物科技有限公司 | Thermal expansion and cold contraction type integrated reaction tube |
| CN113614965A (en) * | 2018-12-04 | 2021-11-05 | 株式会社Lg新能源 | Laminating device, laminating method, and secondary battery manufacturing apparatus including laminating device |
| CN114134033A (en) * | 2021-12-06 | 2022-03-04 | 广东润鹏生物技术有限公司 | PCR thermal cycling device and control method |
| WO2022160998A1 (en) * | 2021-01-29 | 2022-08-04 | 广东润鹏生物技术有限公司 | Molecular diagnostic platform |
| US11654435B2 (en) | 2019-03-29 | 2023-05-23 | Boe Technology Group Co., Ltd. | Detection chip, method for operating detection chip, and reaction system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103135634A (en) * | 2013-01-18 | 2013-06-05 | 上海交通大学 | Temperature control method and device for polymerase chain reaction biochip |
| CN104263634A (en) * | 2014-09-24 | 2015-01-07 | 中国科学技术大学 | Flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and heating method |
-
2016
- 2016-08-22 CN CN201610705502.3A patent/CN106222068A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103135634A (en) * | 2013-01-18 | 2013-06-05 | 上海交通大学 | Temperature control method and device for polymerase chain reaction biochip |
| CN104263634A (en) * | 2014-09-24 | 2015-01-07 | 中国科学技术大学 | Flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and heating method |
Non-Patent Citations (1)
| Title |
|---|
| GUOET AL.: "Polymerase chain reaction chip with microchannel of glass capillaries embedded", 《ELECTRONICS LETTERS》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929409B (en) * | 2017-03-17 | 2019-04-05 | 上海交通大学 | Glass sleeve pipe structure pcr chip array |
| CN106929409A (en) * | 2017-03-17 | 2017-07-07 | 上海交通大学 | Glass sleeve pipe structure pcr chip array |
| CN108865818A (en) * | 2017-05-08 | 2018-11-23 | 广州康昕瑞基因健康科技有限公司 | sequencing reaction device |
| CN109207645A (en) * | 2018-11-01 | 2019-01-15 | 福建省博凯科技有限公司 | The control method of one-part form thermal convection instrument |
| CN109486652A (en) * | 2018-11-12 | 2019-03-19 | 安徽中医药高等专科学校 | A kind of PCR micro-fluidic chip and preparation method thereof |
| CN113614965A (en) * | 2018-12-04 | 2021-11-05 | 株式会社Lg新能源 | Laminating device, laminating method, and secondary battery manufacturing apparatus including laminating device |
| CN113614965B (en) * | 2018-12-04 | 2023-10-31 | 株式会社Lg新能源 | Lamination device and lamination method, and secondary battery manufacturing apparatus including lamination device |
| US11654435B2 (en) | 2019-03-29 | 2023-05-23 | Boe Technology Group Co., Ltd. | Detection chip, method for operating detection chip, and reaction system |
| WO2020207295A1 (en) * | 2019-04-09 | 2020-10-15 | 京东方科技集团股份有限公司 | Reaction device and reaction system for detection chip |
| US11883822B2 (en) | 2019-04-09 | 2024-01-30 | Beijing Boe Technology Development Co., Ltd. | Reaction device for detection chip and reaction system |
| WO2022160998A1 (en) * | 2021-01-29 | 2022-08-04 | 广东润鹏生物技术有限公司 | Molecular diagnostic platform |
| CN113249196A (en) * | 2021-05-06 | 2021-08-13 | 北京谊安和景生物科技有限公司 | Thermal expansion and cold contraction type integrated reaction tube |
| CN114134033A (en) * | 2021-12-06 | 2022-03-04 | 广东润鹏生物技术有限公司 | PCR thermal cycling device and control method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106222068A (en) | Miniature PCR system of capillary glass tube and preparation method thereof | |
| CN107904161B (en) | Micro-fluidic chip for visual immediate detection of pathogen nucleic acid and preparation method and detection method thereof | |
| CN102083533B (en) | Microfluidic chip devices and their use | |
| JP4441653B2 (en) | Automated two-dimensional electrophoresis apparatus and apparatus components | |
| CN102998242B (en) | Micro-fluid cytometer and manufacture method thereof | |
| TW200413097A (en) | Microfluidic device with thin-film electronic devices | |
| CN105505761A (en) | Digital isothermal nucleic acid detecting device and detecting method thereof | |
| CN103071548A (en) | Power source-free and valve-free type single molecule detection chip and applications thereof | |
| CN105296349A (en) | Microfluidic chip, detection system and device used for rapid DNA detection | |
| CN109517732B (en) | Integrated DNA analysis system | |
| CN108636471A (en) | A kind of nucleic acid amplifier and its application | |
| CN202415561U (en) | Quantitative PCR (Polymerase Chain Reaction) microfluidic control chip device | |
| CN104894106A (en) | High-integration equidistance equipartition nucleic acid amplification micro-fluidic chip and application | |
| CN205170857U (en) | A micro -fluidic chip , detecting system and device for DNA short -term test | |
| CN102604824A (en) | Space-oriented mini micro-fluidic real-time fluorescent PCR (Plymerase Chain Reaction) working system | |
| CN102559488A (en) | Quantitative polymerase chain reaction (PCR) microfluidic chip integrated device for integrated electrochemical detection technology | |
| US20220250075A1 (en) | System and method for isolating and analyzing cells | |
| CN111004708B (en) | PCR temperature cycle control method and rotary driving type PCR temperature cycle control device | |
| CN107109333A (en) | PCR device with sliding drive mechanism repeatedly and utilize its PCR method | |
| CN206232730U (en) | The module thermal balance device of PCR gene amplification instrument | |
| CN204490890U (en) | Thermal gradient microreactor | |
| CN111879922A (en) | Integrated paper-based chip structure suitable for synchronous detection of nucleic acid and immunity and manufacturing method thereof | |
| CN101718698A (en) | Laser-induced fluorescence analyzer with PCR-CE coupled microfluidic chip | |
| CN209446605U (en) | A kind of liquid stream control and measuring device based on hydrogel | |
| CN107129933A (en) | One kind is based on electrically driven (operated) digital microcurrent-controlled pcr chip device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |