CN106929409B - Glass sleeve pipe structure pcr chip array - Google Patents
Glass sleeve pipe structure pcr chip array Download PDFInfo
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- CN106929409B CN106929409B CN201710160092.3A CN201710160092A CN106929409B CN 106929409 B CN106929409 B CN 106929409B CN 201710160092 A CN201710160092 A CN 201710160092A CN 106929409 B CN106929409 B CN 106929409B
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- pcr
- glass
- pcr chip
- chip array
- sleeve pipe
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
Abstract
The present invention provides a kind of glass sleeve pipe structure pcr chip array, wherein pcr chip array is made of multiple pcr chips, single pcr chip includes capillary glass tube, glass sock, heating electrode, thermometric electrode, capillary glass tube is nested in glass sock, two heating electrodes are symmetrically distributed in glass sock upper and lower surface, and two thermometric electrodes are symmetrically distributed in glass sock front and rear surfaces；Capillary glass tube is used as PCR reaction chamber when single pcr chip work, and electric current generates heat regulation PCR reaction chamber room temperature by heating electrode, by thermal resistance effect；Thermometric electrode real-time monitoring PCR reaction chamber room temperature；Multiple pcr chips are arranged in formation pcr chip array on bracket.Temperature rate of the present invention is fast, and the system circular response period is short, and thermometric is accurate；Capillary glass tube PCR reaction chamber can avoid generating bubble, protein adsorption；Pcr chip array can carry out the test of multiple reaction systems simultaneously, further increase efficiency.
Description
Technical field
The present invention relates to the pcr chip arrays of PCR field of biotechnology, and in particular, to a kind of glass sleeve pipe structure PCR
Chip array.
Background technique
Polymerase chain reaction (PCR) is a large amount of specific DNA fragments of common external rapid amplifying in field of biomedicine
Protocols in Molecular Biology.PCR cycle reaction includes high temperature unwinding, low-temperature annealing, medium temperature three processes of extension.Conventional PCR
Instrument volume is larger, and price is high；Reaction reagent thermal capacitance is big, so that warming and cooling rate is low, exposure is anti-in a high temperauture environment for a long time
Enzymatic activity is answered to reduce, system reaction efficiency is lower.
The principal element for influencing PCR cycle reaction efficiency includes PCR reaction chamber temperature uniformity, temperature-controlled precision and liter
Rate of temperature fall etc..
That there are temperature uniformities is not high for existing polymer pcr chip, temperature measurement accuracy is poor, amplification efficiency is low, reaction solution contains
The defects of a large amount of bubbles.
Thus, it needs to design the high pcr chip structure of a amplification efficiency at this stage.
Through retrieving, the Chinese invention patent application of Publication No. CN102899238A discloses a kind of continuous flow PCR and hair
The integrated micro flow control chip device of cons electrophoresis function, including continuous-flow PCR chip and capillary electrophoresis chip.But it should
Shortcoming existing for patent is: the not reproducible use of integrated microfluidic chip, at high cost.Publication No. CN104946511A's
Chinese invention patent application discloses temperature sensing pcr chip in a kind of laminated construction chamber, including successively arranges from top to bottom
Upper substrate, thermometric electrode, reaction chamber, lower substrate, heating electrode.But shortcoming existing for the patent is: thermometric electricity
Pole is protruded into reaction solution, so that reaction solution is easily contaminated；Total is non-dismountable, not reproducible use, at high cost.
The Chinese invention patent application of Publication No. CN106222068A discloses a kind of miniature PCR system of capillary glass tube
System and preparation method thereof, the PCR system includes chip carrier, pcr chip, fan.But shortcoming existing for the patent is:
Single heating electrode cannot be guaranteed the uniformity of reacting liquid temperature in pcr chip reaction chamber；Deep trouth open-top leads to thermometric
Electrode thermometric is inaccurate；Multiple reaction systems cannot be tested simultaneously.
Summary of the invention
For the defects in the prior art, the object of the present invention is to provide a kind of glass sleeve pipe structure pcr chip arrays, should
Pcr chip Array Cycle is short reaction time, and temperature uniformity is high, and PCR amplification is high-efficient；Temperature controlled system temperature control accuracy is high, rises
Rate of temperature fall is fast；Reaction solution carries out bubble-free when circular response in PCR reaction chamber；Pcr chip is reusable, reduces and uses
Cost；It can carry out the test of multiple reaction systems simultaneously using array structure.
In order to achieve the above object, the present invention provides a kind of glass sleeve pipe structure pcr chip array, comprising: bracket, pedestal and
Pcr chip array, in which: the bracket is fixed on the base；The pcr chip array is made of multiple pcr chips, more
A mutually isostructural pcr chip is arranged in juxtaposition and is connected on the bracket；
Multiple pcr chips are all made of sleeve pipe structure, comprising: glass sock, glass capillary, two heating electrodes and two
A thermometric electrode, in which: the glass sock is nested with the capillary glass tube；The capillary glass tube is used as pcr chip reaction
Chamber, inside are used for placing response liquid；Two heating electrodes and two thermometric electrodes are respectively symmetrically produced on described
The outer surface of glass sock；
When the heating electrode flows into electric current, it is indoor anti-to adjust the PCR reaction chamber to generate heat by thermal resistance effect
Answer liquid temperature；The indoor reacting liquid temperature of PCR reaction chamber described in the thermometric electrode real-time monitoring；By multiple identical structure PCR
The pcr chip array that chip layout is formed on bracket, for testing a variety of reaction systems simultaneously.
Preferably, the pcr chip is binded on the bracket by adiabatic module.
It is highly preferred that the adiabatic module is made of low thermal conductivity material, for preventing the heating electrode from radiating to institute
State bracket.
Preferably, in the pcr chip, the glass sock and the glass capillary use separate structure, thus just
In disassembly.
It is highly preferred that being cylinder or square duct inside the glass sock.
It is highly preferred that the capillary glass tube is round or square duct.
Preferably, the heating electrode is gathered using snakelike, and two heating electrodes are symmetrically distributed in the upper of glass sock
Lower surface, to maintain the temperature uniformity of reaction solution in the PCR reaction chamber, to accelerate temperature rate.
Preferably, the outer surface of the heating electrode covers layer of insulation material protective layer, to reduce heat loss rate.
Preferably, the thermometric electrode flows into quantitative electric current, the indoor reaction solution temperature of PCR reaction chamber described in real-time monitoring
Degree.
Preferably, the outer surface of the thermometric electrode covers layer of insulation material protective layer, to reduce heat loss rate.
In the present invention, the glass sock, the heating electrode and the thermometric electrode repetitive cycling are used, with reduce at
This；The glass capillary is disposable, to avoid biological pollution.
In the present invention, the PCR reaction chamber good airproof performance of the capillary glass tube production avoids generation and the albumen of bubble
Absorption.
Compared with prior art, the present invention have it is following the utility model has the advantages that
Glass sleeve pipe structure pcr chip array of the present invention realizes glass sock and heating electrode using separate type sleeve pipe structure
With the recycling of thermometric electrode, cost is reduced；
The present invention uses the PCR reaction chamber structure of capillary glass tube production, and good leak tightness avoids bubble generation and albumen
Absorption；
The present invention uses pcr chip array structure, can carry out the test of multiple systems simultaneously；
Overall structure of the invention is small in size, and thermal capacitance is small, and warming and cooling rate is fast.
Detailed description of the invention
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention,
Objects and advantages will become more apparent upon:
Fig. 1 is the overall structure figure of one embodiment of the invention,
In figure: 10,20,30,40 be thermometric electrode, and 11,21,31,41 be heating electrode, and 100,200,300,400 be exhausted
Thermal modules；
Fig. 2 is the structure chart of the glass sleeve pipe structure pcr chip of one embodiment of the invention,
In figure: 101,105 be thermometric electrode pad, and 102,104 interconnect for transition, and 103 be temperature-sensitive resistor, 107,
110 be heating electrode pad, and adding thermal resistance 108,109 is glass sock；
Fig. 3 is the structure chart of the PCR reaction chamber of one embodiment of the invention,
In figure: 106 be capillary glass tube；
Fig. 4 is the structural front view of the glass sleeve pipe structure pcr chip of one embodiment of the invention；
10,12 be thermometric electrode, and 11,13 be heating electrode.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection scope.
As shown in Figure 1, a kind of glass sleeve pipe structure pcr chip array, by pedestal, bracket, multiple glass sleeve pipe structure PCR
Chip composition, in which: the bracket is fixed on the base；Multiple glass sleeve pipe structure pcr chips are arranged in juxtaposition shape
At pcr chip array, and adiabatic module 100,200,300,400 is respectively adopted with the bracket and sticks together.
As a preferred embodiment, the bracket is made of good plastic material.
As a preferred embodiment, the material conducts heat rate that the adiabatic module 100,200,300,400 uses is low, with
The heating electrode is effectively prevented to distribute heat to the bracket.
Multiple and column distribution glass sleeve pipe structure pcr chip specific compositions and indifference, details are as shown in Figure 2.
As shown in figs 2-4, the structure of multiple glass sleeve pipe structure pcr chips is identical, comprising: capillary glass tube
106, glass sock 109 heats electrode 11,13, thermometric electrode 10,12；Wherein:
The capillary glass tube 106 (as shown in Figure 3) is nested in glass sock 109 (as shown in Figure 2), capillary glass tube
106 are used as PCR reaction chamber；The heating electrode 11,13 is located at 109 upper and lower faces of glass sock and is symmetric (as schemed
Shown in 4)；The thermometric electrode 10,12 is located at the forward and backward two sides of glass sock 109 and is symmetric (as shown in Figure 4).
As shown in figure 4, the heating electrode 13 is identical with heating 11 specific compositions of electrode.
For heating electrode 11, as shown in Fig. 2, the heating electrode 11 is welded by heating electrode pad 107, heating electrode
Disk 110 and adding thermal resistance 108 form, in which: the heating electrode pad 107 and the heating electrode pad 110 are symmetrical
At the both ends of the adding thermal resistance 108.
As preferred embodiment, as shown in Fig. 2, the serpentine-like distribution of the adding thermal resistance 108, passes through heating electrode weldering
Disk 107, heating electrode pad 110 give 108 constant current of adding thermal resistance, to realize to the capillary glass tube 106
The heating of PCR reaction chamber, and guarantee being uniformly distributed for PCR reaction chamber room temperature.
When the heating electrode 11,13 flows into different electric currents, by thermal resistance effect, reaction solution in PCR reaction chamber is adjusted
Temperature, PCR reaction chamber respectively enters different workspaces, i.e. PCR reaction chamber is at different temperatures region, glass pipe sleeve
Structure pcr chip will be worked respectively high temperature untwists, cryogenic absorption, medium temperature extend these three workspaces.
As shown in figure 4, the heating electrode 11 and the heating electrode 13 can be simultaneously to the capillary glass tubies 106
Reaction solution heats in PCR reaction chamber, improves heating efficiency.
As shown in figure 4, the thermometric electrode 12 is identical with 10 concrete composition of thermometric electrode,
By taking thermometric electrode 10 as an example, as shown in Fig. 2, thermometric electrode pad 101, thermometric of the thermometric electrode 10 by both ends
Electrode pad 105, transition interconnection 102, transition interconnection 104 and temperature-sensitive resistor 103 form；Wherein: the temperature is sensitive
Resistance 103 is located at the middle position of thermometric electrode 10, the transition interconnection 102 and the thermometric electrode pad 101, the transition
Interconnection 104 and the thermometric electrode pad 105 are successively symmetrically distributed in the both sides of the temperature-sensitive resistor 103.
The thermometric electrode 10 and the thermometric electrode 12 can reduce pcr chip simultaneously to PCR reaction chamber measuring temperature
Real time temperature error.
As preferred embodiment, as shown in Fig. 2, passing through the thermometric electrode pad 101, thermometric electrode pad 105
Give the temperature-sensitive resistor 103 constant input current through transition interconnection 102, transition interconnection 104, by Ohm's law
The voltage at 103 both ends of temperature-sensitive resistor, and then real-time measurement PCR reaction chamber room temperature can be demarcated.
As preferred embodiment, the glass sock 109 and the capillary glass tube 106 are sleeve pipe structure, and the two can
With separation；
The glass sock 109, the heating electrode 11, heating electrode 13, the thermometric electrode 10, thermometric electrode 12
It recycles.
As preferred embodiment, the glass sleeve pipe structure pcr chip connects firmly forms array on the bracket, can
To carry out the test of multiple reaction systems.
Glass sleeve pipe structure pcr chip array of the present invention, will heating electrode and thermometric electrode respectively with external circuit
It is connected, accesses data acquisition unit；Digital computer controls whole system reaction process, can real-time control and observing response into
Degree.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring substantive content of the invention.
Claims (8)
1. a kind of glass sleeve pipe structure pcr chip array characterized by comprising bracket, pedestal and pcr chip array,
In: the bracket is fixed on the base；The pcr chip array is made of multiple pcr chips, multiple mutually isostructural
Pcr chip is arranged in juxtaposition and is connected on the bracket；
Multiple pcr chips are all made of sleeve pipe structure, comprising: glass sock, glass capillary, two heating electrodes and two surveys
Warm electrode, in which: the glass sock is nested with the capillary glass tube；The capillary glass tube is used as pcr chip reaction chamber,
Inside is used for placing response liquid；Two heating electrodes and two thermometric electrodes are respectively symmetrically produced on the glass sock
Outer surface；
When the heating electrode flows into electric current, heat is generated to adjust the indoor reaction solution of PCR reaction chamber by thermal resistance effect
Temperature；The indoor reacting liquid temperature of PCR reaction chamber described in the thermometric electrode real-time monitoring；By multiple identical structure pcr chips
It is arranged in formation pcr chip array on bracket, for testing a variety of reaction systems simultaneously.
2. a kind of glass sleeve pipe structure pcr chip array according to claim 1, which is characterized in that the pcr chip is logical
Cross adiabatic module bonding on the bracket；The adiabatic module is made of low thermal conductivity material, for preventing the heating electricity
It radiates to the bracket pole.
3. a kind of glass sleeve pipe structure pcr chip array according to claim 1, which is characterized in that the pcr chip
In, the glass sock and the glass capillary use separate structure, consequently facilitating disassembly.
4. a kind of glass sleeve pipe structure pcr chip array according to claim 3, which is characterized in that in the glass sock
Portion is cylinder or square duct.
5. a kind of glass sleeve pipe structure pcr chip array according to claim 3, which is characterized in that the glass capillary
Pipe is round or square duct.
6. a kind of glass sleeve pipe structure pcr chip array according to claim 1, which is characterized in that the heating electrode
It is gathered using snakelike, two heating electrodes are symmetrically distributed in the upper and lower surface of glass sock, to maintain the PCR reaction chamber
The temperature uniformity of interior reaction solution, to accelerate temperature rate.
7. a kind of glass sleeve pipe structure pcr chip array according to claim 1, which is characterized in that the heating electrode
Layer of insulation material protective layer is covered with the outer surface of the thermometric electrode, to reduce heat loss rate.
8. a kind of glass sleeve pipe structure pcr chip array according to claim 1, which is characterized in that the thermometric electrode
Flow into quantitative electric current, the indoor reacting liquid temperature of PCR reaction chamber described in real-time monitoring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710160092.3A CN106929409B (en) | 2017-03-17 | 2017-03-17 | Glass sleeve pipe structure pcr chip array |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710160092.3A CN106929409B (en) | 2017-03-17 | 2017-03-17 | Glass sleeve pipe structure pcr chip array |
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| Publication Number | Publication Date |
|---|---|
| CN106929409A CN106929409A (en) | 2017-07-07 |
| CN106929409B true CN106929409B (en) | 2019-04-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710160092.3A Active CN106929409B (en) | 2017-03-17 | 2017-03-17 | Glass sleeve pipe structure pcr chip array |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109385467A (en) * | 2018-10-17 | 2019-02-26 | 东南大学 | A kind of multiplexed PCR amplification method based on elongated reaction chamber |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101004287A (en) * | 2006-01-19 | 2007-07-25 | 博奥生物有限公司 | Heating up parts of capillary |
| CN102899238A (en) * | 2012-07-19 | 2013-01-30 | 凯晶生物科技(苏州)有限公司 | Micro fluidic chip apparatus by integrating continuous flow PCR and capillary electrophoresis function |
| CN106222068A (en) * | 2016-08-22 | 2016-12-14 | 上海交通大学 | Miniature PCR system of capillary glass tube and preparation method thereof |
-
2017
- 2017-03-17 CN CN201710160092.3A patent/CN106929409B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101004287A (en) * | 2006-01-19 | 2007-07-25 | 博奥生物有限公司 | Heating up parts of capillary |
| CN102899238A (en) * | 2012-07-19 | 2013-01-30 | 凯晶生物科技(苏州)有限公司 | Micro fluidic chip apparatus by integrating continuous flow PCR and capillary electrophoresis function |
| CN106222068A (en) * | 2016-08-22 | 2016-12-14 | 上海交通大学 | Miniature PCR system of capillary glass tube and preparation method thereof |
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| CN106929409A (en) | 2017-07-07 |
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