CN115747025B - Full-automatic nucleic acid extraction and PCR detection all-in-one - Google Patents
Full-automatic nucleic acid extraction and PCR detection all-in-one Download PDFInfo
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- CN115747025B CN115747025B CN202211260269.4A CN202211260269A CN115747025B CN 115747025 B CN115747025 B CN 115747025B CN 202211260269 A CN202211260269 A CN 202211260269A CN 115747025 B CN115747025 B CN 115747025B
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Abstract
The utility model belongs to the technical field of nucleic acid extraction equipment, and in particular relates to a full-automatic nucleic acid extraction and PCR detection integrated machine which comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the nucleic acid extraction module and the PCR detection module are arranged in the machine shell; the nucleic acid extraction module comprises a consumable unit, a pipetting unit and a magnetic adsorption unit, wherein the consumable unit is used for storing consumables required by nucleic acid extraction and PCR reaction; the pipetting unit is arranged between the consumable unit and the PCR detection module and is used for picking up the tip head and sucking the reagent and the sample in the consumable unit into the tip head through the tip head; the magnetic adsorption unit is arranged below the pipetting unit and comprises a magnet, and the magnet is close to the tip head to perform magnetic adsorption. The utility model can realize repeated adsorption to the pyrolysis liquid for many times and recover the magnetic beads, so that the content of the magnetic beads in the waste liquid is controllable; the number of times of extracting the nucleic acid is not limited by the structure, and is favorable for recovering the magnetic beads and subsequent washing and amplifying operations.
Description
Technical Field
The utility model belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a full-automatic nucleic acid extraction and PCR detection integrated machine.
Background
Nucleic acid extraction is an essential link in various molecular biology experiments, and almost every laboratory requires nucleic acid extraction in connection with separation and purification of biomolecules. The nucleic acid extraction instrument is an instrument for automatically completing the nucleic acid extraction work by using matched nucleic acid extraction reagents, and is widely applied to the fields of clinical disease diagnosis, microbiological detection and the like; nucleic acid extraction is followed by PCR detection, a molecular biological technique used to amplify specific DNA fragments. At present, two extraction methods, namely a centrifugal column method and a magnetic bead method, are mainly adopted for nucleic acid extraction, the magnetic bead method is a novel nucleic acid extraction technology which takes nano biological magnetic beads as carriers, nucleic acid molecules can be specifically identified and combined with silicon hydroxyl groups on the surfaces of the magnetic beads, and aggregation or dispersion occurs under the action of an external magnetic field, so that the nucleic acid separation and purification are carried out. Currently, magnetic bead nucleic acid extraction generally comprises four main steps of cleavage, binding, washing and elution, and each step can be realized by a plurality of different methods singly or in combination.
The nucleic acid extraction equipment in the prior art is a nucleic acid extraction and PCR detection integrated machine as disclosed in Chinese patent CN113388507B, and comprises a shell, a nucleic acid extraction module and a PCR detection module, wherein the shell is a hollow shell, and the nucleic acid extraction module and the PCR detection module are arranged inside the shell. And a magnetic adsorption device for nucleic acid extraction disclosed in the prior art CN215906208U comprises a mounting bottom plate, wherein a motor is arranged below the mounting bottom plate, and a magnet fixing seat is arranged above the mounting bottom plate; the output shaft of the motor is connected with a screw rod, and the screw rod penetrates through the mounting bottom plate to be connected with the magnet fixing seat; a row of equidistant grooves are formed in the magnet fixing seat, and magnets are embedded in the grooves; the magnet fixing seat is characterized in that a Hall induction magnet is arranged below the magnet fixing seat, and a Hall sensor is arranged below the mounting bottom plate corresponding to the position of the Hall induction magnet. The utility model automatically realizes the dispersion and aggregation of the magnetic beads; meanwhile, the starting and stopping of the motor are accurately controlled by the Hall sensor, so that the accurate position control of the magnet is realized.
Above-mentioned prior art's magnetism adsorption component all is located the kit below, and through drive arrangement drive magnetism adsorption component up-and-down reciprocating motion, be close to the lysate and realize magnetism adsorption, its step includes: adding a lysate to the sample, blowing and mixing uniformly, adsorbing the beads, removing the lysate, adding a washing solution to wash the beads, adsorbing the beads, removing the washing solution, adding an elution buffer and the like, thereby obtaining purified nucleic acid. However, in the magnetic adsorption mode, after the cleavage is completed, the waste liquid needs to be removed, and the removed waste liquid is placed in a waste liquid pipe far away from the magnet; in order to remove the waste liquid thoroughly as possible, the pipetting tip needs to extend into the bottom of the deep-hole plate, if the tip is too close to the magnetic beads, the magnetic beads are sucked away together with the waste liquid, then the magnetic beads in the waste liquid cannot be removed again through magnetic adsorption, the requirement of the content of the magnetic beads in the waste liquid cannot be met, and the magnetic beads are reduced, so that the subsequent washing and nucleic acid amplification are affected.
In summary, the magnetic adsorption module in the prior art cannot realize repeated and repeated adsorption, and is easy to influence subsequent washing and nucleic acid amplification.
Disclosure of Invention
The utility model provides a full-automatic nucleic acid extraction and PCR detection integrated machine, which aims to solve the technical problems that a magnetic adsorption component in the prior art can not realize repeated and repeated adsorption and is easy to influence subsequent washing and nucleic acid amplification.
In order to achieve the above purpose, the technical scheme of the utility model is as follows:
the full-automatic nucleic acid extraction and PCR detection integrated machine comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the nucleic acid extraction module and the PCR detection module are arranged in the machine shell, and a lifting door assembly for separation is arranged between the nucleic acid extraction module and the PCR detection module; the nucleic acid extraction module comprises a consumable unit, a pipetting unit and a magnetic adsorption unit, wherein the consumable unit is used for storing consumables required by nucleic acid extraction and PCR reaction; the pipetting unit is arranged between the consumable unit and the PCR detection module and is used for picking up the tip head and sucking the reagent and the sample in the consumable unit into the tip head through the tip head; the magnetic adsorption unit is arranged below the pipetting unit and comprises a magnet, and the magnet is close to the tip head for magnetic adsorption.
Further, the pipetting unit comprises a support frame, the support frame is fixed on the casing, a tip head is arranged on one side of the support frame, and the tip head reciprocates up and down along the support frame; the lower end of the supporting frame is provided with the magnetic adsorption unit; the magnetic adsorption unit comprises a mounting plate, and the mounting plate is fixedly connected with the support frame.
Further, the magnetic adsorption unit further comprises a linear driving device, a guide assembly and a magnet fixing plate, wherein the linear driving device is fixedly connected to the mounting plate and is used for driving the magnet fixing plate to reciprocate; the guide assembly comprises a guide shaft and a linear bearing penetrating through the guide shaft, the guide shaft is penetrated through the magnet fixing plate, and the linear bearing is fixedly connected with the magnet fixing plate; the magnet is arranged on the magnet fixing plate.
Furthermore, a fixing frame is arranged below the mounting plate, folded edges bent downwards are arranged at two ends of the fixing frame, and the guide shafts are fixed on the folded edges at two ends of the fixing frame.
Furthermore, a spring is arranged on the guide shaft in a penetrating way, one end of the spring is in contact with the linear bearing, and the other end of the spring is in contact with the folded edge far away from the magnet.
Still further, the straight line drive arrangement includes motor, gear and rack, the motor sets up on the mounting panel, the output fixed connection of motor the gear and drive gear rotation, rack and gear engagement, the rack with magnet fixed plate fixed connection.
Furthermore, the magnet fixing plate is provided with a plurality of grooves at equal intervals on the side surface close to the tip head, the magnets are arranged in the grooves, and the magnets are in one-to-one correspondence with the tip head.
Further, a first air inlet is formed in the upper portion of the shell, a first air outlet is formed in the lower portion of the rear side of the shell, a second air inlet is formed in the upper portion of the first air outlet, and a second air outlet is formed in the upper portion of the second air inlet; a first air duct is arranged between the lower part of the nucleic acid extraction module and the bottom wall of the shell, and is used for air inlet from a first air inlet and air outlet from a first air outlet through the nucleic acid extraction module;
the bottom of the shell is provided with a third air inlet, external air is fed in from the third air inlet and is converged into the air path behind the first air duct, and then is discharged from the first air outlet;
the PCR detection module is internally provided with a second air duct, the second air duct is used for air intake from a second air inlet and air exhaust from a second air outlet through the PCR detection module.
Further, the first air inlet is provided with a first filter screen, the first air outlet is provided with a second filter screen, the second air inlet is provided with a third filter screen, and the second air outlet is provided with a fourth filter screen.
Further, the first filter screen is fixed on the inner wall above the shell through a frame.
Further, a sealing plate is arranged between the upper part of the nucleic acid extraction module and the casing, the right end of the sealing plate is bent upwards and is connected with the casing, and the left end of the sealing plate extends downwards, so that external air enters the casing from the first air inlet and then enters the consumable unit downwards along the sealing plate; a first air duct is arranged below the consumable unit, a fifth filter screen is arranged at the left end of the first air duct, and circulated air enters the first air duct from the fifth filter screen;
the right-hand member in first wind channel sets up the air-blower, and first wind channel right side sets gradually wind channel baffle, fan, cold water radiator, second filter screen.
Further, a third air inlet is formed in the casing below the air duct baffle, external air enters from the third air inlet and sequentially passes through the air duct baffle, the fan, the cold water radiator and the second filter screen, and air is discharged from the first air outlet.
Further, a gun discharging support is arranged on one side of the support frame and is in sliding connection with the support frame, a plurality of connecting pipes are arranged on the gun discharging support, and the tip head is arranged below the connecting pipes;
and a pipetting motor is fixed above the middle part of the support frame and drives the gun rack to reciprocate up and down through a transition assembly.
Further, the transition assembly comprises an adapter plate, and the pipetting motor is connected with the adapter plate through a lead screw and drives the adapter plate to move up and down; two ends of the adapter plate are arranged on the gun row support through buffer components.
Still further, the buffering subassembly includes set screw and wears to establish the spring on the set screw, the keysets both ends set up the fixed orifices, set screw passes the fixed orifices of keysets and is connected with the row rifle support.
Furthermore, a faller is arranged below the gun rack, a plurality of through holes are formed in the faller, and the connecting pipes are arranged in the through holes in a penetrating manner; the needle withdrawing motor is arranged on the supporting frame and drives the needle withdrawing plate to move up and down through the screw rod.
Compared with the prior art, the utility model has the following beneficial effects:
the utility model sets the magnetic adsorption unit under the liquid transfer unit, after adsorbing samples, magnetic beads and the like into the tip head through the liquid transfer unit, drives the magnetic adsorption unit to realize magnetic adsorption close to the tip head, discharges waste liquid into the reagent tube after the magnetic beads are adsorbed to one side of the tip head, and then prevents the residual magnetic beads from carrying out subsequent operation in the washing liquid, if the waste liquid contains the magnetic beads, the tip head can be utilized to absorb the waste liquid again for re-adsorption, and the magnetic beads are recovered, so that the content of the magnetic beads in the waste liquid is controllable; compared with the limit that only one adsorption can be carried out in the prior art, the number of times of extracting the nucleic acid is not limited by the structure, and the method is favorable for recovering the magnetic beads and subsequent washing and amplifying operations.
According to the utility model, by arranging the three air duct systems, the nucleic acid extraction module, the PCR detection module and the PCR water-cooling circulation system are respectively ventilated, so that the reagents of the nucleic acid extraction module and the PCR detection module are prevented from being polluted by air, and the effectiveness of an experiment process is ensured; and the stable operation of the PCR water cooling circulation system is ensured, and the stable operation of the temperature raising and lowering assembly in the PCR detection module is ensured. And meanwhile, an air cooling loop of the cold water radiator is overlapped with the first air duct, so that the volume and the cost of air cooling are reduced.
According to the utility model, the support frame is driven by the pipetting motor through the transition assembly, the pipetting motor does not directly drive the support frame, when the screw rod driven by the pipetting motor swings radially, the adapter plate is inclined along with the screw rod, the spring can buffer and correct the force transmitted by the adapter plate, the influence of the radial swing of the screw rod on the support frame is reduced, and the possibility of inclination of the support frame is further reduced, so that the tip head can pipetting smoothly.
Drawings
Fig. 1 is a schematic structural view of the present utility model.
Fig. 2 is a schematic diagram of the internal structure of the present utility model.
FIG. 3 is a schematic diagram of the structure of the pipetting unit and the magnetic adsorption unit according to the utility model.
Fig. 4 is a schematic structural view of a pipetting unit according to the utility model.
Fig. 5 is a schematic structural diagram of the magnetic adsorption unit according to the present utility model.
FIG. 6 is a schematic diagram of the wind tunnel of the present utility model.
FIG. 7 is a schematic diagram showing the structure of a nucleic acid extraction module according to the present utility model.
Fig. 8 is an exploded view of the first air duct according to the present utility model.
Fig. 9 is a schematic structural view of a transition assembly according to the present utility model.
Reference numerals illustrate:
1-casing, 2-consumable unit, 3-pipetting unit, 301-support frame, 302-tip head, 303-gun rack, 304-connecting tube, 305-pipetting motor, 306-adapter plate, 307-screw, 308-fixing screw, 309-spring, 310-needle withdrawal plate, 311-needle withdrawal motor, 312 screw nut,
4-magnetic adsorption unit, 401-magnet, 402-mounting plate, 403-magnet fixing plate, 404-guide shaft, 405-linear bearing, 406-fixing frame, 407-spring, 408-motor, 409-gear, 410-rack, 411-optocoupler induction piece, 412-optocoupler, 413-end seat,
the device comprises a first air inlet 5-a first air outlet 6-a first air outlet 7-a second air inlet 8-a second air outlet 9-a third air inlet 10-a first filter screen 11-a second filter screen 12-a third filter screen 13-a fourth filter screen 14-a frame 15-a closing plate 16-a fifth filter screen 16-a fixing plate 17-a fixing plate 18-a blower 19-an air duct baffle 20-a fan 21-a cold water radiator 22-a PCR detection module 23-a fan 24-a first air duct 25-a cover plate.
Detailed Description
The technical solutions of the present utility model will be clearly described below with reference to the accompanying drawings, and it is obvious that the described embodiments are not all embodiments of the present utility model, and all other embodiments obtained by a person skilled in the art without making any inventive effort are within the scope of protection of the present utility model.
And the terms "left", "right", "upper" and "lower" are used in the present utility model to describe structural features in conjunction with the drawings, and do not limit the scope of the present utility model.
The utility model provides a full-automatic nucleic acid extraction and PCR detection integrated machine, which comprises a machine shell 1, a nucleic acid extraction module and a PCR detection module 22, wherein the nucleic acid extraction module and the PCR detection module 22 are arranged in the machine shell 1, as shown in fig. 2, the nucleic acid extraction module is arranged on the left side of the machine shell 1, and the PCR detection module 22 is arranged on the right side of the machine shell 1; a lifting door component for separation is arranged between the nucleic acid extraction module and the PCR detection module 22; the nucleic acid extraction module comprises a consumable unit 2, a pipetting unit 3 and a magnetic adsorption unit 4, wherein the consumable unit 2 is arranged at the leftmost side of the nucleic acid extraction module and is used for storing consumables required by nucleic acid extraction and PCR reaction, and the consumables comprise a reagent, a tip head, a reaction tube, a cracking tube, a nuclear lifting tube and the like; the pipetting unit 3 is arranged between the consumable unit 2 and the PCR detection module 22, and is used for picking up the tip head 302 and sucking the reagent and the sample in the consumable unit 2 into the tip head through the tip head 302; the magnetic adsorption unit 4 is arranged below the pipetting unit 3, the magnetic adsorption unit 4 comprises a magnet 401, and the magnet 401 is close to the tip head 302 for magnetic adsorption.
In the process of extracting nucleic acid, if different nucleic acids are extracted at the same time, the types of magnetic beads and adsorption time used for extracting nucleic acids are different, and in the prior art, when different nucleic acids are extracted at the same time, the experiment with the longest magnetic adsorption time is often considered, so that the experiment with shorter magnetic adsorption time needs too long magnetic adsorption time, which results in that the nucleic acid extraction experiment cannot be performed at the same time, or the experimental result is poor. The utility model can repeat magnetic adsorption, so that experiments with shorter magnetic adsorption time can be considered, after experiments with shorter magnetic adsorption time are completed, waste liquid or residual liquid can be transferred through the tip head, and then the waste liquid with longer magnetic adsorption time is re-adsorbed, and the experiments are completed again. Furthermore, the utility model can be compatible with various types of nucleic acid extraction experiments, can be simultaneously carried out, greatly reduces the experiment time and lowers the experiment cost.
As shown in fig. 3 and 4, the pipetting unit 3 includes a support 301, two ends of the support 301 are fixed on the casing 1 by screws, two sets of linear guide rails are disposed on the left side of the support 301, the linear guide rails are disposed along the vertical direction, two sliding blocks are disposed on each linear guide rail, and the gun stand 303 is fixedly connected with the sliding blocks through a fixing plate, so that the gun stand 303 slides relative to the support 301 along the vertical direction. The row gun bracket 303 is provided with a row of equidistant through holes, a connecting pipe 304 is fixed in the through holes, the connecting pipe 304 protrudes out of the lower surface of the row gun bracket 303, the lower end of the connecting pipe 304 is connected with a tip head 302, and the outer surface of the lower end of the connecting pipe 304 is also provided with two O-shaped rings, so that the connection of the connecting pipe 304 and the tip head 302 is facilitated.
The tip head 302 can reciprocate up and down relative to the support 301 along with the gun row bracket 303; the lower end of the supporting frame 301 is provided with the magnetic adsorption unit 4; as shown in fig. 5, the magnetic adsorption unit 4 includes a mounting plate 402, and the mounting plate 402 is fixedly connected with the support 301.
Specifically, the magnetic attraction unit 4 further includes a magnet fixing plate 403, a rack and pinion driving mechanism, and a guide assembly. The mounting panel 402 top sets up motor 408, and motor 408's output shaft passes mounting panel 402 downwardly extending, fixed gear 409 on motor 408's the output shaft, gear 409 are located mounting panel 402 below, and gear 409 and rack 410 meshing, rack 410 and magnet fixed plate 403 fixed connection, the both ends of magnet fixed plate 403 respectively set up a set of guide assembly, guide assembly includes guiding axle 404 and wears to locate the linear bearing 405 on the guiding axle 404, guiding axle 404 wears to establish on the magnet fixed plate 403, linear bearing 405 with magnet fixed plate 403 fixed connection.
A fixing frame 406 is arranged below the mounting plate 402, folded edges bent downwards are arranged at two ends of the fixing frame 406, and two ends of the guide shaft 404 are fixed on the folded edges at two ends of the fixing frame 406. The motor 408 drives the magnet fixing plate 403 to reciprocate along the guide shaft 404 through a rack and pinion mechanism to achieve approaching or separating from the tip head 302.
The guide shaft 404 is provided with a spring 407 in a penetrating manner, one end of the guide shaft 404, which is far away from the magnet 401, is provided with an end seat 413, one end of the spring 407 is in contact with the linear bearing 405, and the other end of the spring 407 is in contact with the end seat 413. The purpose of the spring 407 is to prevent the magnet fixing plate 403 from deflecting by the force of the spring, which may cause inaccurate correspondence between the magnet and the tip head 302, and affect magnetic attraction.
The magnet fixing plates 403 are provided with a row of grooves at equal intervals near the side surfaces of the tip heads 302, the magnets 401 are arranged in the grooves, and the magnets are in one-to-one correspondence with the tip heads 302. An optocoupler 412 is arranged on the lower surface of the fixing frame 406, a connecting plate is arranged on the magnet fixing plate 403, an optocoupler sensing piece 411 is arranged on the connecting plate, and the corresponding arrangement of the optocoupler 412 and the optocoupler sensing piece 411 sequentially detects the moving distance of the magnet fixing plate 403.
As shown in fig. 1 and 6, a first air inlet 5 is arranged above the casing 1, a first air outlet 6 is arranged below the rear side of the casing 1, a second air inlet 7 is arranged above the first air outlet 6, and a second air outlet 8 is arranged above the second air inlet 7; a first air duct 24 is arranged between the lower part of the nucleic acid extraction module and the bottom wall of the shell 1, the first air duct 24 is used for air intake from the first air inlet 5 and air exhaust from the first air outlet 6 through the nucleic acid extraction module;
the bottom of the shell 1 is provided with a third air inlet 9, external air enters the air channel at the rear side of the first air channel 24 from the third air inlet 9, and then is discharged from the first air outlet 6; as in fig. 6 the different arrows indicate the different air flow directions.
The PCR detection module is internally provided with a second air duct, the second air duct is used for air intake from the second air inlet 7, and air is exhausted from the second air outlet 8 through the PCR detection module 22. The influence of external air on the PCR detection module is effectively blocked, and meanwhile, the influence of polluted air generated in the PCR detection process on the external environment is effectively avoided.
The first air inlet 5 is provided with a first filter screen 10, the first air outlet 6 is provided with a second filter screen 11, the second air inlet 7 is provided with a third filter screen 12, and the second air outlet 8 is provided with a fourth filter screen 13.
As shown in fig. 7 and 8, the first filter screen 10 is fixed to the inner wall above the cabinet 1 by a frame 14. A sealing plate 15 is arranged between the upper part of the nucleic acid extraction module and the casing 1, the right end of the sealing plate 15 is bent upwards and is connected with the casing 1, and the left end of the sealing plate 15 extends downwards, so that external air enters the casing 1 from the first air inlet 5 and then enters the consumable unit 2 downwards along the sealing plate 15; a first air duct 24 is arranged below the consumable unit 2, a fifth filter screen 16 is arranged at the left end of the first air duct 24, the fifth filter screen 16 is fixedly connected to the first air duct 24 through a fixing plate 17, and circulated air enters the first air duct 24 from the fifth filter screen 16; the right-hand member of first wind channel 24 sets up air-blower 18, and air-blower 18 top closes the wind channel through apron 25, and first wind channel 24 right side sets gradually wind channel baffle 19, fan 20, cold water radiator 21, second filter screen 11.
A fan 23 is arranged below the consumable unit 2, the fan 23 can promote air entering from the first air inlet 5 to quickly enter the first air duct 24, and the air entering the first air duct 24 is sequentially discharged from the first air outlet 6 through the air duct baffle 19, the fan 20, the cold water radiator 21 and the second filter screen 11. Thereby effectively blocking the influence of air outside the instrument on nucleic acid extraction and simultaneously effectively avoiding the pollution of polluted air generated in the nucleic acid extraction process to the external environment.
The casing 1 below the air duct baffle 19 is provided with a third air inlet 9, external air enters from the third air inlet 9 and sequentially passes through the air duct baffle 19, the fan 20, the cold water radiator 21 and the second filter screen 11, and air is discharged from the first air outlet 6. The smoothness of the whole water cooling circulation system is ensured, and the risk of blockage of the PCR circulation efficiency is reduced.
As shown in fig. 4 and 9, a pipetting motor 305 is fixed above the middle of the support 301, and the pipetting motor 305 drives the gun rack 303 to reciprocate up and down through a transition assembly. The transition assembly comprises an adapter plate 306, and the pipetting motor 305 is connected with the adapter plate 306 through a lead screw 307 and a lead screw nut 312 and drives the adapter plate 306 to move up and down; two ends of the adapter plate 306 are arranged on the gun rack 303 through buffer components. The buffering assembly comprises a fixing screw 308 and a spring 309 penetrating through the fixing screw 308, fixing holes are formed in two ends of the adapter plate 306, and the fixing screw 308 penetrates through the fixing holes of the adapter plate 306 to be connected with the gun rack 303.
A needle withdrawing plate 310 is arranged below the gun rack 303, a plurality of through holes are arranged on the needle withdrawing plate 310, and the connecting pipe 304 is arranged in the through holes in a penetrating manner; the needle withdrawing motor 311 is arranged on the supporting frame 301, and the needle withdrawing motor 311 drives the needle withdrawing plate to move up and down through a screw rod. Specifically, two sides of the pipetting motor 305 are respectively provided with a needle withdrawing motor 311, an output shaft of the needle withdrawing motor 311 is a screw rod, the screw rod of the needle withdrawing motor 311 is in threaded connection with the needle withdrawing plate 310, and the needle withdrawing motor 311 can drive the needle withdrawing plate 310 to move up and down. the diameter of the connection position of the tip head 302 and the connection pipe 304 is larger than the diameter of the through hole on the faller plate 310, so that when the faller plate 310 moves downwards, the faller plate 310 can push the tip head 302 to move downwards, and the tip head 302 is separated from the connection pipe 304.
The above embodiments are only for illustrating the technical solution of the present utility model and not for limiting the same, and although the present utility model has been described in detail with reference to examples, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the scope of the technical solution of the present utility model, which is intended to be covered by the claims of the present utility model.
Claims (9)
1. The full-automatic nucleic acid extraction and PCR detection integrated machine comprises a machine shell, a nucleic acid extraction module and a PCR detection module, wherein the nucleic acid extraction module and the PCR detection module are arranged in the machine shell, and a lifting door assembly for separation is arranged between the nucleic acid extraction module and the PCR detection module; the nucleic acid extraction module is characterized by comprising a consumable unit, a pipetting unit and a magnetic adsorption unit, wherein the consumable unit is used for storing consumables required by nucleic acid extraction and PCR reaction; the pipetting unit is arranged between the consumable unit and the PCR detection module and is used for picking up the tip head and sucking the reagent and the sample in the consumable unit into the tip head through the tip head; the magnetic adsorption unit is arranged below the pipetting unit and comprises a magnet, and the magnet is close to the tip head to perform magnetic adsorption;
the pipetting unit comprises a support frame, the support frame is fixed on the casing, a tip head is arranged on one side of the support frame, and the tip head reciprocates up and down along the support frame; the lower end of the supporting frame is provided with the magnetic adsorption unit; the magnetic adsorption unit comprises a mounting plate, and the mounting plate is fixedly connected with the support frame;
the magnetic adsorption unit further comprises a linear driving device, a guide assembly, a magnet fixing plate and a magnet, wherein the linear driving device is fixedly connected to the mounting plate and is used for driving the magnet fixing plate to reciprocate; the guide assembly comprises a guide shaft and a linear bearing penetrating through the guide shaft, the guide shaft is penetrated through the magnet fixing plate, and the linear bearing is fixedly connected with the magnet fixing plate; the magnet is arranged on the magnet fixing plate;
a fixing frame is arranged below the mounting plate, folded edges bent downwards are arranged at two ends of the fixing frame, and the guide shafts are fixed on the folded edges at two ends of the fixing frame; a spring is arranged on the guide shaft far away from the linear driving device in a penetrating way, one end of the spring is contacted with the linear bearing, and the other end of the spring is contacted with the folded edge far away from the magnet; the linear driving device comprises a motor, a gear and a rack, the motor is arranged on the mounting plate, the output end of the motor is fixedly connected with the gear and drives the gear to rotate, the rack is meshed with the gear, and the rack is fixedly connected with the magnet fixing plate;
the magnet fixing plate is close to the side face of the tip head, a plurality of grooves are formed in the side face of the tip head at equal intervals, magnets are arranged in the grooves, and the magnets correspond to the tip head one by one.
2. The full-automatic nucleic acid extraction and PCR detection integrated machine according to claim 1, wherein a first air inlet is formed in the upper side of the machine shell, a first air outlet is formed in the lower side of the rear side of the machine shell, a second air inlet is formed in the upper side of the first air outlet, and a second air outlet is formed in the upper side of the second air inlet; a first air duct is arranged between the lower part of the nucleic acid extraction module and the bottom wall of the shell, and is used for air inlet from a first air inlet and air outlet from a first air outlet through the nucleic acid extraction module;
the bottom of the shell is provided with a third air inlet, external air is fed in from the third air inlet and is converged into the air path behind the first air duct, and then is discharged from the first air outlet;
the PCR detection module is internally provided with a second air duct, the second air duct is used for air intake from a second air inlet and air exhaust from a second air outlet through the PCR detection module.
3. The full-automatic nucleic acid extraction and PCR detection integrated machine according to claim 2, wherein a first filter screen is arranged at the first air inlet, a second filter screen is arranged at the first air outlet, a third filter screen is arranged at the second air inlet, and a fourth filter screen is arranged at the second air outlet.
4. The full-automatic nucleic acid extraction and PCR detection integrated machine according to claim 3, wherein a sealing plate is arranged between the upper part of the nucleic acid extraction module and the machine shell, the right end of the sealing plate is bent upwards and connected with the machine shell, and the left end of the sealing plate extends downwards, so that external air enters the machine shell from the first air inlet and then enters the consumable unit downwards along the sealing plate; a first air duct is arranged below the consumable unit, a fifth filter screen is arranged at the left end of the first air duct, and circulated air enters the first air duct from the fifth filter screen;
the right-hand member in first wind channel sets up the air-blower, and first wind channel right side sets gradually wind channel baffle, fan, cold water radiator, second filter screen.
5. The full-automatic nucleic acid extraction and PCR detection integrated machine according to claim 4, wherein a third air inlet is formed in a casing below the air duct baffle, external air enters from the third air inlet, sequentially passes through the air duct baffle, the fan, the cold water radiator and the second filter screen, and is discharged from the first air outlet.
6. The full-automatic nucleic acid extraction and PCR detection integrated machine according to claim 1, wherein a gun discharging support is arranged on one side of the support frame, the gun discharging support is in sliding connection with the support frame, a plurality of connecting pipes are arranged on the gun discharging support, and the tip head is arranged below the connecting pipes;
and a pipetting motor is fixed above the middle part of the support frame and drives the gun rack to reciprocate up and down through a transition assembly.
7. The full-automatic nucleic acid extraction and PCR detection integrated machine according to claim 6, wherein the transition assembly comprises an adapter plate, and the pipetting motor is connected with the adapter plate through a screw rod and drives the adapter plate to move up and down; two ends of the adapter plate are arranged on the gun row support through buffer components.
8. The full-automatic nucleic acid extraction and PCR detection integrated machine according to claim 7, wherein the buffer assembly comprises a fixing screw and a spring penetrating through the fixing screw, fixing holes are formed in two ends of the adapter plate, and the fixing screw penetrates through the fixing holes of the adapter plate to be connected with the gun rack.
9. The full-automatic nucleic acid extraction and PCR detection integrated machine according to claim 8, wherein a faller is arranged below the gun rack, a plurality of through holes are formed in the faller, and the connecting pipe is arranged in the through holes in a penetrating manner;
the needle withdrawing motor is arranged on the supporting frame and drives the needle withdrawing plate to move up and down through the screw rod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211260269.4A CN115747025B (en) | 2022-10-14 | 2022-10-14 | Full-automatic nucleic acid extraction and PCR detection all-in-one |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211260269.4A CN115747025B (en) | 2022-10-14 | 2022-10-14 | Full-automatic nucleic acid extraction and PCR detection all-in-one |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115747025A CN115747025A (en) | 2023-03-07 |
| CN115747025B true CN115747025B (en) | 2023-09-22 |
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Family Applications (1)
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