CN211112037U - High-flux nucleic acid extraction system - Google Patents

Abstract

The utility model belongs to the technical field of it is biological, concretely relates to high flux nucleic acid extraction system. The utility model provides a high flux nucleic acid extraction system, sweep sign indicating number device and controlling means including operation storehouse, manipulator, bar magnet, magnetic sleeve, reaction unit, extraction element, liquid distributor, sample, the manipulator includes but at least two three-dimensional motion's third axle, and extraction element movably sets up on a third axle, loads the subassembly including the first that is used for fixed bar magnet and the second that is used for fixed magnetic sleeve to make the bar magnet stretch into the magnetic sleeve or shift out by the magnetic sleeve and make the magnetic sleeve stretch into reaction unit or shift out by reaction unit respectively; the liquid distribution device is movably arranged on another third shaft and comprises at least two distribution needles to be switched between two states of extending into the reaction device and removing from the reaction device respectively. The manipulator is under the controlling means effect, and the running accuracy is high, and stability is good, and the sign indicating number device is swept to the sample automated identification simultaneously, has improved nucleic acid extraction efficiency.

Description

High-flux nucleic acid extraction system

Technical Field

The utility model belongs to the technical field of it is biological, concretely relates to high flux nucleic acid extraction system.

Background

Nucleic acids are an important class of biological macromolecules for storing and transmitting vital information, and are also core molecules of modern biochemical and molecular biological research. With the rapid development of molecular biology in recent years, a large number of molecular biology experiments based on nucleic acid, such as second-generation sequencing, fluorescence quantitative PCR, gene chips, nucleic acid molecular hybridization, etc., have emerged, and the integrity of DNA, the degradation degree of RNA, and the purity and concentration of nucleic acid, etc., all have a greater or lesser effect on the results of molecular biology experiments, so the problem in molecular biology experiments is how to extract nucleic acid with high purity from complex samples quickly and efficiently.

Various nucleic acid extraction methods such as phenol-chloroform extraction, alkali extraction, cetyltrimethylammonium bromide (CTAB) extraction, silica gel membrane adsorption, magnetic bead separation, and the like exist, and a nucleic acid extractor based on the above methods has been developed. However, as the biological industry develops, the number of samples for nucleic acid extraction tends to increase and the complexity tends to increase, and in order to meet the trend, the development of a high-throughput nucleic acid extractor is a hot spot nowadays. Wherein, the nucleic acid separator based on the magnetic bead separation method can be divided into a suction type and a magnetic rod type according to the automatic extraction process, and the two main differences are as follows: the nucleic acid extractor based on the suction type realizes the extraction and purification of nucleic acid by transferring a reaction solution, and comprises the following steps: adding lysis solution into a sample, blowing and uniformly mixing, repeatedly adsorbing by magnetic beads, removing the lysis solution, adding washing solution to wash the magnetic beads, adsorbing the magnetic beads, removing the washing solution, adding eluent and the like to obtain purified nucleic acid, wherein liquid residue exists at the bottom in the liquid transferring process, so that the salt residue is large, the purity of the nucleic acid is relatively low, and if the sample is too viscous, an automatic liquid transferring system for absorbing the liquid is easy to block; the nucleic acid extractor based on the magnetic rod method comprises a magnetic rod, a magnetic sleeve, a deep hole plate and the like, and the steps of the nucleic acid extractor comprise: when extracting nucleic acid, adding a sample into a deep-hole plate for lysis, simultaneously adsorbing the nucleic acid in a lysis solution to the surface of the magnetic bead by the magnetic bead contained in the system, and then transferring the magnetic bead with the nucleic acid by a magnetic rod to finish the impurity washing process in different deep-hole plates, the extracted nucleic acid has low residual salt content and higher purity, and the problem of blockage does not occur, but the traditional nucleic acid extractor based on the magnetic rod method has the following problems: 1) the purified nucleic acid eluent needs to be transferred manually, and manual operation errors are easy to occur in the transferring process, so that the pollution of human factors is caused, and false positives are generated; 2) through salt washing, washing and elution, the steps are multiple, the time consumption is long, particularly when a large number of samples need to be processed in a short time, the cross contamination is serious, and the error rate is greatly improved; 3) in the process of transferring magnetic beads for many times, nucleic acid adsorbed on the magnetic beads is exposed outside and is easily polluted by impurities in the environment, such as dust, enzyme and the like, and especially RNA is easily degraded; 4) the equipment market price is higher, the maintenance cost is high, and each functional module harmony, flexibility are relatively poor.

SUMMERY OF THE UTILITY MODEL

Therefore, the to-be-solved technical problem of the utility model lies in overcoming current nucleic acid extraction appearance based on bar magnet method because of degree of automation is high inadequately, thereby each functional module harmony and flexibility are relatively poor lead to draw the defect that the step is loaded down with trivial details, the error rate is high, there is sample pollution etc to provide a high flux nucleic acid extraction system that each functional module flexibility and integratability are better, draw the step simple, the error rate is low.

In order to solve the technical problem, the utility model discloses a technical scheme is:

the utility model provides a high flux nucleic acid extraction system, which comprises an operation cabin for providing a closed environment, and a manipulator, a magnetic bar, a magnetic sleeve, a reaction device, an extraction device, a liquid distribution device, a sample code sweeping device and a control device which are arranged in the operation cabin;

wherein the manipulator comprises at least two third axes movable in three dimensions; the extraction device comprises a first loading assembly and a second loading assembly, wherein the first loading assembly and the second loading assembly are movably arranged on one third shaft respectively and used for fixing the magnetic rod and the magnetic sleeve so as to enable the magnetic rod to extend into or move out of the magnetic sleeve and enable the magnetic sleeve to extend into or move out of the reaction device respectively; the liquid distribution device comprises at least two distribution needles which are respectively movably arranged on the other third shaft so as to be switched between two states of extending into the reaction device and removing from the reaction device; the sample code scanning device comprises a code scanning mechanism and an automatic auxiliary mechanism so as to automatically identify the sample; the control device is connected with the manipulator.

Preferably, the high throughput nucleic acid extraction system, the first loading assembly comprises a first n-shaped frame and at least one first fixing rod detachably disposed in the first n-shaped frame; the second loading assembly comprises a second n-shaped frame and at least one second fixing rod detachably arranged in the second n-shaped frame.

Further preferably, in the high-throughput nucleic acid extraction system, the first loading assembly is disposed above the second loading assembly, and both are connected to the third shaft through a slide rail.

Further preferably, the high-throughput nucleic acid extraction system, the liquid distribution device further comprises a driving mechanism and an adjusting mechanism arranged on the driving mechanism, the adjusting mechanism comprises at least two gears and a toothed plate respectively engaged with each gear, and the distribution needle is arranged on the toothed plate.

Further preferably, in the high-flux nucleic acid extraction system, the adjusting mechanism includes three-order reference circle gears with sequentially increasing diameters and corresponding three-order toothed plates, the three-order gears are coaxially driven by the driving mechanism, and the three-order toothed plates respectively perform dislocation motion under the driving of each-order gear to adjust the spacing between the distribution needles.

Further preferably, the reaction device of the high-throughput nucleic acid extraction system comprises an array pore channel mechanism, the automatic auxiliary mechanism comprises a plurality of clamping seats for driving the array pore channel mechanism to rotate, limiting holes for limiting the array pore channel mechanism and a moving track, and the code scanning mechanism moves along the moving track to automatically identify the rotating array pore channel mechanism.

Further preferably, the high throughput nucleic acid extraction system, the liquid dispensing device further comprises a syringe pump mechanism coupled to the dispensing needle.

Further preferably, the high-throughput nucleic acid extraction system comprises two first shafts arranged in parallel, two second shafts movably arranged on the first shafts, and two third shafts movably arranged on each of the second shafts.

Preferably, the high-throughput nucleic acid extraction system further comprises a heating and oscillating mechanism, a droplet capturing mechanism, a cleaning and drying device and a disinfection and sterilization device which are arranged in the operation chamber and connected with the control device.

Further preferably, the high-throughput nucleic acid extraction system, the cleaning and drying device comprises a cleaning mechanism and a drying mechanism.

The utility model discloses technical scheme has following advantage:

1. the utility model provides a high flux nucleic acid extraction system, sweep a yard device and controlling means including operation storehouse and manipulator, bar magnet, magnetic sleeve, reaction unit, extraction element, liquid distribution device, the sample of setting in this operation storehouse.

The high-flux nuclear extraction system with the structure comprises a manipulator, a reaction device and a magnetic rod, wherein the manipulator comprises at least two third shafts capable of moving in three dimensions, an extraction device is movably arranged on one of the third shafts, and the extraction device comprises a first loading assembly for fixing the magnetic rod and a second loading assembly for fixing a magnetic sleeve so as to respectively extend the magnetic rod into the magnetic sleeve or move the magnetic rod out of the magnetic sleeve and extend the magnetic sleeve into the reaction device or move the magnetic sleeve out of the reaction device; on the other third shaft a liquid dispensing device is movably arranged, which comprises at least two dispensing needles to be switched in two states of being inserted into or removed from the reaction device, respectively. The manipulator can accurately realize the relative motion between bar magnet and the magnetic sleeve and the liquid-transfering operation of each distribution needle single channel under the control device effect, and the running precision is high, and stability is good, and the sign indicating number device is swept to the sample automated identification to the sample information is typeeed to the sample simultaneously, has improved the efficiency that the nucleic acid drawed.

2. The utility model provides a high flux nucleic acid extraction system, first loading subassembly includes first n type frame and can dismantle the first dead lever of being connected with this first n type frame, the second loading subassembly includes second n type frame and can dismantle the second dead lever of being connected with this second n type frame, the loading mechanism of this structure can selectively load and carry out the experiment with a plurality of sets of bar magnets and the magnetic sleeve that nucleic acid extraction experiment flux is equivalent to can realize the three-dimensional motion of bar magnet and magnetic sleeve in order to carry out the nucleic acid extraction process under the drive of third axle.

3. The utility model provides a high flux nucleic acid extraction system, adjustment mechanism includes the gear that third-order reference circle diameter increases gradually and third-order pinion rack with every order gear correspondence ground, third-order gear is by actuating mechanism coaxial drive, then third-order pinion rack produces the dislocation motion under every order gear drive respectively, make a plurality of distribution needles that set up on every order pinion rack take place relative motion, also, the accurate regulation can be realized to the interval between a plurality of distribution needles, the flexibility is good, the degree of freedom is high, can match the reaction unit of different intervals.

4. The utility model provides a high flux nucleic acid extraction system still catches mechanism, washing drying device and disinfection and isolation device including setting up in the operation storehouse and the heating oscillation mechanism that is connected with controlling means, liquid drop, leads to the structure that realizing high flux nucleic acid that controlling means can automize draws, compares with current like product, has better flexibility and higher integrated nature.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural diagram of a high-throughput nucleic acid extraction system provided in embodiment 1 of the present invention;

FIG. 2 is a schematic view of the internal structure of FIG. 1;

FIG. 3 is a top view of the base of FIG. 1;

FIG. 4 is a schematic view of the electrical control principle of the control device of the high-throughput nucleic acid extraction system provided in embodiment 1 of the present invention;

FIG. 5 is a schematic view of the structure of the heating oscillating mechanism and the droplet capturing mechanism;

FIG. 6 is an exploded view of the heating oscillating assembly of FIG. 1;

FIG. 7 is a schematic view showing a state in which the magnetic rod and the magnetic sleeve are hung on the hanging rack in FIG. 1;

FIG. 8 is a schematic view of the magnetic rod and the magnetic sleeve of FIG. 1 fixed on the loading mechanism;

FIG. 9 is a schematic view of the liquid dispensing apparatus of FIG. 1;

FIG. 10 is a schematic view of the adjustment mechanism of FIG. 9;

FIG. 11 is a schematic structural diagram of the code scanning mechanism in FIG. 1;

FIG. 12 is a schematic diagram of the internal structure of the code scanning mechanism in FIG. 11;

FIG. 13 is a schematic view of the structure of the washing and drying apparatus shown in FIG. 1;

FIG. 14 is a schematic view of the internal structure of the washing and drying apparatus shown in FIG. 13;

description of reference numerals:

1-operating a bin; 11-a base; 12-a chassis; 121-window; 13-windowing; 131-a window;

2-a control device; 21-a power switch; 22-emergency stop button; 23-a PC terminal;

3-a manipulator; 31-a first axis; 32-a second axis; 33-a third axis;

4-a reaction device; 41-array pore channel mechanism; 42-a heating oscillating mechanism; 421-a first housing; 422-heating the oscillating assembly; 4221-a first base; 42211-first groove; 4222-an electromagnet; 4223-a vibrating mass; 4224-cover plate; 4225-heat insulation pad; 4226-hot plate; 4227-heat conducting plate; 4228-limit rack;

5-an extraction device; 51-a magnetic bar; 52-magnetic sleeve; 53-hanging rack; 54-a loading mechanism; 541-a first loading assembly; 5411-first n-type frame; 5412-first fixation bar; 542-a second loading assembly; 5421-second n-type frame; 5422-second fixation bar; 55-a droplet capture mechanism; 551-concave disk; 552-supporting feet; 56-low temperature preservation mechanism;

6-a liquid dispensing device; 61-a second housing; 611-fixing strips; 62-a drive mechanism; 63-an adjustment mechanism; 631-a gear assembly; 632-toothed plate assembly; 64-a dispensing needle; 65-a syringe pump mechanism;

7-a sample code scanning device; 71-a code scanning mechanism; 72-automated assistance mechanism; 721-a third housing; 722-a servo motor; 723-a pulley; 724-cassette; 725-limiting hole; 726-motion track;

8, cleaning and drying the device; 81-a fourth shell; 82-a cleaning mechanism; 82-a drying mechanism; 84-a sewage draining pipe;

9-a disinfection and sterilization device.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Furthermore, the technical features mentioned in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other.

Example 1

The embodiment provides a high-throughput nucleic acid extraction system, as shown in fig. 1, comprising an operation cabin 1, a control device 2, a manipulator 3, a reaction device 4, an extraction device 5, a liquid distribution device 6, a sample code scanning device 7, a cleaning and drying device 8 and a disinfection and sterilization device 9.

As shown in fig. 1, 2 and 3, the operation cabin 1 includes a base 11, a cabinet 12 and a window 13. A plurality of accommodating grooves are formed in the base 11; the chassis 12 is disposed on the base 11, and a window 121 is disposed on a front case of the chassis 12; the upper edge of the window 13 is hinged to the upper edge of the window 121, a window 131 is arranged on the window 13, and the window 131 is made of glass. Through window 13 lock window 121 on in order to keep apart nucleic acid extraction process and external environment, reduce the influence of external environment to nucleic acid extraction, and can look over the nucleic acid extraction process in real time through window 131.

As shown in fig. 1, the control device 2 includes a motion control unit, a temperature control unit, a data transmission unit, a display drive unit, a power switch 21, an emergency stop button 22, and a PC terminal 23. As shown in fig. 4, the motion control unit communicates with the manipulator 3, the reaction device 4, the extraction device 5, the liquid distribution device 6 and the sample code scanning device 7 through a communication protocol of RS232 or RS485, so as to realize the control of precise manipulator motion, heating oscillation, extraction operation, liquid distribution and automatic code scanning; the temperature control unit is used for controlling the temperatures of the reaction device 4, the extraction device 5 and the cleaning and drying device 8, heating or cooling power devices in the reaction device 4, the extraction device 5 and the cleaning and drying device 8 are respectively controlled to keep constant temperature, and the stability precision is controlled to be +/-0.5 ℃. The data of the control device 2 is uploaded to the upper computer through the data transmission unit to realize data recording and analysis, and temperature information display or basic interactive operation information is realized on the liquid crystal display screen through the display driving unit.

As shown in fig. 2, the robot 3 includes a first shaft 31, a second shaft 32, and a third shaft 33. The first shaft 31 comprises two parallel arms, two ends of each arm are erected on the base 11, and the plane where the two parallel arms are located is parallel to the upper surface of the base 11; the second shaft 32 comprises two parallel arms which are perpendicular to the first shaft 31, two ends of each arm are respectively in sliding lap joint with the two arms of the first shaft 31, and can independently reciprocate along the first shaft 31 in the horizontal X direction under the instruction of a motion control unit of the control device 2; the third shaft 33 includes two parallel arms vertically disposed, and disposed on the two arms of the second arm 32, respectively, and can independently reciprocate in the horizontal Y direction along the second shaft 32 or independently reciprocate in the vertical Z direction along the second shaft 32 under the instruction of the motion control unit of the control device 2. In the robot 3 having such a structure, the end of the third shaft 33 facing the base 11 is used for fixing the extraction device 5, the liquid distribution device 6, and the like, and the third shaft 33 can move in the three-dimensional directions XYZ under the instruction of the movement control unit of the control device 2, and can precisely move to any position above the base 11, thereby improving the automation degree of nucleic acid extraction.

As shown in fig. 2 and 3, the reaction device 4 includes an array well mechanism 41 and a heating oscillation mechanism 42. The array pore channel mechanism 41 selects a porous plate or a plurality of connecting pipes, and each pore channel can be used as the occasion of cracking, washing and eluting in the nucleic acid extraction process; the multi-connection tube can be selected from 6-hole plates, 12-hole plates, 24-hole plates, 48-hole plates and 96-hole plates, and the multi-connection tube can be selected from 6-hole channels, 12-hole channels, 24-hole channels, 48-hole channels and 96-hole channels.

As shown in fig. 5 and 6, the heating oscillating mechanism 42 includes a first housing 421 and a plurality of sets of heating oscillating assemblies 422 disposed on the first housing 421, each set of heating oscillating assemblies 422 includes a first base 4221, an electromagnet 4222, a vibrating block 4223, a cover plate 4224, a heat insulating pad 4225, a heating plate 4226, a heat conducting plate 4227, and a limit bracket 4228. The first base 4221 is provided with 4 pieces of first grooves 42211, electromagnets 4222 are respectively arranged around the first grooves 42211, and a vibrating block 4223 is arranged in the first grooves 42211 and is in sliding connection with the first grooves 42211, and is made of a ferromagnetic material, as shown in fig. 7; by turning on and off the 4 electromagnets 4222, the vibrating mass 4223 is attracted to move back and forth in the first groove 42211 in different directions, and oscillation is formed. A cover plate 4224, a heat insulation pad 4225, a heating plate 4226, a heat conduction plate 4227 and a limit frame 4228 are sequentially arranged on the vibrating block 4223; the heat insulation pad 4225 reduces the influence of heat of the heating plate 4226 on the electromagnet 4222 and the vibrating block 4223; the heating plate 4226 is connected with the temperature control unit by a heating blanket; the limiting frame 4228 is used for preventing the array channel mechanism 41 from being arranged, and the heat of the heating plate 4226 is transferred to the array channel mechanism 41 through the heat conducting plate 4227. The heating oscillation mechanism 42 of this structure controls the oscillation state such as the oscillation mode, direction, speed and oscillation interval time by the motion control unit, and the temperature control unit controls the temperature in real time, thereby satisfying the diversified heating oscillation requirements in the nucleic acid extraction process.

As shown in fig. 1, 2, 3, 5, 7, and 8, the extraction device 5 includes a magnetic rod 51, a magnetic sleeve 52, a hanging rack 53, a loading mechanism 54, a droplet capturing mechanism 55, and a cryopreservation mechanism 56. A hanging rack 53 is provided on the base 11 for hanging the magnetic rod 51 and the magnetic sleeve 52 for use in the extraction operation. The loading mechanism 54 is disposed on a third shaft 33, and includes a first loading assembly 541 and a second loading assembly 542 disposed below the first loading assembly 541; the first loading assembly 541 comprises a first n-shaped frame 5411 and a plurality of first fixing rods 5412 arranged in the first n-shaped frame 5411 and used for hanging magnetic rods 51, the first fixing rods 5412 are detachably connected with the first n-shaped frame 5411, the first n-shaped frame 5411 is connected with the third shaft 33 through sliding rails, 8 magnetic rods 51 can be hung on each first fixing rod 5412, 12 first fixing rods 5412 can be arranged on the first n-shaped frame 5411 in parallel, that is, 96 magnetic rods 51 can be hung on the first loading assembly 541; the second loading assembly 542 includes a second n-shaped frame 5421 and a plurality of second fixing rods 5422 arranged in the second n-shaped frame 5421 and used for hanging the magnetic sleeves 52, the second fixing rods 5422 are detachably connected with the second n-shaped frame 5421, the second n-shaped frame 5421 is connected with the third shaft 33 through sliding rails, 8 magnetic sleeves 52 can be hung on each second fixing rod 5422, 12 second fixing rods 5422 can be arranged on the second n-shaped frame 5421 in parallel, that is, 96 magnetic sleeves 52 can be hung on the second loading assembly 542. The first loading assembly 541 and the second transferring assembly 542 can independently move relatively under the instruction of the motion control unit of the control device 2, specifically: when the first loading assembly 541 moves relative to the second loading assembly 542, the magnetic rod 51 is extended into the corresponding magnetic sleeve 52 or the magnetic rod 51 is removed from the magnetic sleeve 52; when the second loading unit 542 moves relative to the array tunnel mechanism 41, the magnetic sleeve 52 is extended into the tunnel of the array tunnel mechanism 41 or the magnetic sleeve 52 is removed from the tunnel of the array tunnel mechanism 41. As shown in fig. 5, the liquid capturing mechanism 55 includes a concave plate 551 and a supporting leg 552 disposed on the lower surface of the concave plate 551, the concave plate 551 corresponds to a set of heating oscillating assemblies 422, the supporting leg 552 is slidably connected to the first housing 421, and the liquid capturing mechanism 55 can move back and forth above the heating oscillating mechanism 42 under the command of the motion control unit of the control device 2 to capture the liquid dropped from the magnetic rod 51 and the magnetic sleeve 52 when the magnetic rod 51 is inserted into or removed from the corresponding magnetic sleeve 52 and the magnetic sleeve 52 is inserted into or removed from the aperture of the array aperture mechanism 41, so as to avoid cross contamination. The nucleic acid extract may be temporarily stored in the cryopreservation mechanism 56, and the cryopreservation mechanism 56 is connected to the temperature control unit of the control apparatus 2 to provide a suitable storage temperature.

As shown in fig. 9 and 10, the liquid dispensing device 6 includes a second housing 61, a driving mechanism 62, an adjusting mechanism 63, a dispensing needle 64, and a syringe pump mechanism 65. The second housing 61 is disposed at one end of the other third shaft 33 facing the base 11, a strip-shaped opening (not shown) and two fixing strips 611 are disposed on a side wall of the second housing 61, and the fixing strips 611 are disposed in the same direction as the strip-shaped opening to support the adjusting mechanism 63. The driving mechanism 62 is fixedly disposed in the second housing 61, and a motor is selected, a rotating shaft of the motor is vertically disposed downward, and the operation state, such as rotating speed and steering, of the motor is controlled by a motion control unit of the control device 2. The adjusting mechanism 63 comprises a gear assembly 631 and a toothed plate assembly 632, wherein the gear assembly 631 is located in the second housing 61, is composed of three-step gears which are sequentially stacked, and is concentrically arranged on the rotating shaft of the driving mechanism 62, and the reference circle diameters of the three-step gears are sequentially increased progressively from top to bottom; the toothed plate assembly 632 is composed of three-step toothed plates stacked in sequence, and the three-step toothed plate portion extends into the second housing 61 through the strip-shaped opening to be meshed with the three-step toothed plates one by one respectively. The three-order toothed plates are respectively arranged on the two fixing strips 611 and can be connected in a sliding manner along the fixing strips 611; one distribution needle 64 is respectively arranged on one fixing strip 611 and each step toothed plate, and 4 distribution needles 64 are arranged in total; the gear assembly 631 is coaxially driven by the driving mechanism 62, and due to the different reference circle diameters of the three-step gears, the amplitude of the dislocation motion of each step of toothed plate along the fixing strip 611 is different, so that the distance between the distribution pins 64 is accurately adjustable to adapt to the array hole mechanism 41 with different sizes. As shown in fig. 1, 2 and 3, the syringe pump mechanism 65 is disposed on the base 11 and includes a plurality of precision syringe pumps, and the distribution needles 64 are connected to the precision syringe pumps in a one-to-one correspondence.

As an alternative embodiment, the gear assembly 631 can also consist of a three-step gear wheel, and correspondingly the toothed plate assembly 632 consists of two-step toothed plates, on each of which one dispense needle 64 is arranged, i.e. a total of two dispense needles 64.

As shown in fig. 1, 2, 3, 11 and 12, the sample code scanning device 7 includes a code scanning mechanism 71 and an automated auxiliary mechanism 72. Wherein, the code scanning mechanism 71 adopts a code scanning gun; the automation assistance mechanism 72 includes a third housing 721, a servomotor 722 provided in the third housing 721, a plurality of pulleys 723 driven by the servomotor 722, a cassette 724 provided on each pulley 723, a stopper hole 725 provided on an upper surface of the third housing 721, and a movement track 726 provided on a side surface of the third housing 721. The servo motor 722 drives a coaxial belt wheel 723 to rotate under the instruction of the motion control unit, and the belt wheel 723 drives other belt wheels 733 to rotate at the same speed through a belt, so that each clamping seat 724 can rotate uniformly; the multiple connecting pipes of the array pore channel mechanism 41 are fixed on each clamping seat 724, are limited by a limiting hole 725 and rotate synchronously with the clamping seats 724; the code scanning mechanism 71 moves on the moving track 726 along the direction parallel to the multi-connected pipe under the instruction of the motion control unit, so as to realize the automatic code scanning operation of each multi-connected pipe bar code.

As shown in fig. 1, 2, 3, 13 and 14, the washing and drying device 8 includes a fourth housing 81, a washing mechanism 82, a drying mechanism 83 and a soil discharge pipe 84. The cleaning mechanism 82 is a cavity structure with an open top end, the structure and the number of the cleaning mechanism are set according to the structure and the number of the distribution needles 64, cleaning liquid, such as deionized water, is sprayed out through the distribution needles 64, firstly, the inner wall surfaces of the distribution needles 64 are cleaned, and then the sprayed cleaning liquid forms vortex in the narrow cavity structure of the cleaning mechanism 82 so as to clean the outer wall surfaces of the distribution needles 64; the bottom of the cavity structure of the cleaning mechanism 82 is provided with a drain pipe 84 which is controlled to be communicated with the cavity structure through a valve, the cleaned sewage is finally discharged by the drain pipe 84 in a unified way, and the cleaned distribution needle 64 extends into the drying mechanism 83 for hot air drying.

As shown in figures 1 and 2, the disinfection and sterilization device 9 is arranged on the inner wall surface of the case 12, comprises two groups of ultraviolet lamps, disinfects the nucleic acid extraction environment, is non-contact sterilization, does not produce impurities, and does not pollute the operation cabin 1.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made without departing from the scope of the invention.

Claims (10)

1. A high-flux nucleic acid extraction system is characterized by comprising an operation cabin for providing a closed environment, and a manipulator, a magnetic rod, a magnetic sleeve, a reaction device, an extraction device, a liquid distribution device, a sample code scanning device and a control device which are arranged in the operation cabin;

wherein the manipulator comprises at least two third axes movable in three dimensions; the extraction device comprises a first loading assembly and a second loading assembly, wherein the first loading assembly and the second loading assembly are movably arranged on one third shaft respectively and used for fixing the magnetic rod and the magnetic sleeve so as to enable the magnetic rod to extend into or move out of the magnetic sleeve and enable the magnetic sleeve to extend into or move out of the reaction device respectively; the liquid distribution device comprises at least two distribution needles which are respectively movably arranged on the other third shaft so as to be switched between two states of extending into the reaction device and removing from the reaction device; the sample code scanning device comprises a code scanning mechanism and an automatic auxiliary mechanism so as to automatically identify the sample; the control device is connected with the manipulator.

2. The high throughput nucleic acid extraction system of claim 1, wherein the first loading assembly comprises a first n-frame and at least one first fixing rod removably disposed within the first n-frame; the second loading assembly comprises a second n-shaped frame and at least one second fixing rod detachably arranged in the second n-shaped frame.

3. The high throughput nucleic acid extraction system of claim 2, wherein the first loading assembly is disposed above the second loading assembly and each is coupled to the third shaft via a slide rail.

4. The high throughput nucleic acid extraction system of claim 3, wherein the liquid dispensing device further comprises a driving mechanism and an adjusting mechanism disposed on the driving mechanism, the adjusting mechanism comprising at least two steps of gears and toothed plates respectively engaged with the gears of each step, the dispensing needle being disposed on the toothed plates.

5. The high-throughput nucleic acid extraction system according to claim 4, wherein the adjusting mechanism comprises three gears with sequentially increasing pitch circle diameters and corresponding three toothed plates, the three gears are coaxially driven by the driving mechanism, and the three toothed plates are respectively driven by the gears at each step to perform a dislocation motion so as to adjust the spacing between the distribution needles.

6. The high-throughput nucleic acid extraction system according to claim 5, wherein the reaction device comprises an array pore channel mechanism, the automatic auxiliary mechanism comprises a plurality of clamping seats for driving the array pore channel mechanism to rotate, a limiting hole for limiting the array pore channel mechanism, and a moving track, and the code scanning mechanism moves along the moving track to automatically identify the rotating array pore channel mechanism.

7. The high throughput nucleic acid extraction system of claim 6, wherein the liquid dispensing device further comprises a syringe pump mechanism coupled to the dispensing needle.

8. The high-throughput nucleic acid extraction system according to claim 7, wherein said manipulator comprises two first shafts disposed in parallel, two second shafts movably disposed on said first shafts, and two said third shafts movably disposed on each of said second shafts.

9. The high-throughput nucleic acid extraction system according to any one of claims 1 to 8, further comprising a heating and shaking mechanism, a droplet capturing mechanism, a washing and drying device and a sterilizing and disinfecting device which are disposed in the operation chamber and connected to the control device.

10. The high throughput nucleic acid extraction system of claim 9, wherein the wash drying device comprises a wash mechanism and a drying mechanism.

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