CN110257372A - A kind of instrument for extracting nucleic acid - Google Patents

A kind of instrument for extracting nucleic acid Download PDF

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Publication number
CN110257372A
CN110257372A CN201910696199.9A CN201910696199A CN110257372A CN 110257372 A CN110257372 A CN 110257372A CN 201910696199 A CN201910696199 A CN 201910696199A CN 110257372 A CN110257372 A CN 110257372A
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China
Prior art keywords
sleeve
nucleic acid
instrument
fixedly connected
extracting nucleic
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Granted
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CN201910696199.9A
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CN110257372B (en
Inventor
方向红
颜友荣
王琳琳
陈光明
戴丽红
徐婷婷
孟婷
刘玉华
高月秀
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Jiangsu Agri Animal Husbandry Vocational College
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Jiangsu Agri Animal Husbandry Vocational College
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Extraction Or Liquid Replacement (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kind of instrument for extracting nucleic acid, including extraction apparatus ontology, air inlet pipe is communicated on the left of the extraction apparatus ontology, the inside of the air inlet pipe is fixedly connected with fixing sleeve by connecting plate, is communicated with positioning pipe on the right side of the extraction apparatus ontology.The present invention twitches the air of extraction apparatus body interior by turning set and positioning pipe by blower, extraneous air is set to enter the inside of extraction apparatus ontology by air inlet pipe and air inlet duct, purified treatment is carried out by gas of the air cleaning unit to extraction again, to have the advantages of capable of carrying out extraction purification to the gas of volatilization, it solves existing instrument for extracting nucleic acid and does not have certain gas treatment means, when opening instrument for extracting nucleic acid, the gas that reagent volatilizes, which will release, causes air pollution, and it is retained in the portion gas in instrument for extracting nucleic acid and is then easy the problem of polluting to the reagent for extracting nucleic acid next time, improve the practicability of instrument for extracting nucleic acid.

Description

A kind of instrument for extracting nucleic acid
Technical field
The present invention relates to extraction apparatus technical field, specially a kind of instrument for extracting nucleic acid.
Background technique
Instrument for extracting nucleic acid is the instrument for being automatically performed sample nucleic acid using matched nucleic acid extracting reagent and extracting work, extensively It is general to apply in Center for Disease Control, clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detection, food safety The multiple fields such as detection, animal husbandry and molecular biology research.
Nucleic acid be by many nucleotide polymerizations at large biological molecule compound, be life one of most basic substance, and Instrument for extracting nucleic acid would generally be used during nucleic acid extraction to extract, but existing instrument for extracting nucleic acid does not have certain gas Body processing mechanism, when opening instrument for extracting nucleic acid, the gas that reagent volatilizes, which will release, causes air pollution, and is retained in core Portion gas in sour extraction apparatus is then easy to pollute the reagent for extracting nucleic acid next time, reduces the reality of instrument for extracting nucleic acid The property used.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of instrument for extracting nucleic acid, having can be to the gas of volatilization The advantages of carrying out extraction purification, solves existing instrument for extracting nucleic acid and does not have certain gas treatment means, is opening nucleic acid When extraction apparatus, the gas that reagent volatilizes, which will release, causes air pollution, and is retained in the portion gas in instrument for extracting nucleic acid Then it is easy the problem of polluting to the reagent for extracting nucleic acid next time.
In order to achieve the above object, the invention provides the following technical scheme: a kind of instrument for extracting nucleic acid, including extraction apparatus sheet Body is communicated with air inlet pipe on the left of the extraction apparatus ontology, and the inside of the air inlet pipe is fixedly connected with fixation by connecting plate It covers, is communicated with positioning pipe on the right side of the extraction apparatus ontology, it is net that the top on the right side of the extraction apparatus ontology is fixedly installed with air Makeup is set, the blower being fixedly connected at the top of positioning pipe on the right side of the extraction apparatus ontology, and the output end of the blower is logical Connecting tube connection is crossed in the left side of air cleaning unit bottom, the inner transverse of the positioning pipe is provided with connecting rod, the company Sealing shroud is fixedly connected on the left of extension bar surface, the surface of the sealing shroud is contacted with the inner wall of positioning pipe, the positioning pipe It is threaded with drive sleeve on the right side of inner cavity, turning set, the input terminal of the blower are socketed on the right side of the drive sleeve surface It is connected to by bellows on the right side of turning set.Positioning pipe is connected to the nucleic acid extraction room of conventional instrument for extracting nucleic acid.
Preferably, the air inlet pipe is internally provided with sealing mechanism, and the sealing mechanism includes sleeve, backing plate, air inlet Slot, rubber sleeve one and tension spring, the sleeve are slidably connected at the inside of fixing sleeve, and the backing plate is fixedly connected on the right side of sleeve Side, the air inlet duct are provided with the right side of air inlet inside pipe wall top and bottom, and the tension spring is fixedly connected on the right side of sleeve lining Side, the left end of the tension spring are fixedly connected on the left side of fixing sleeve inner wall, and the rubber sleeve one is fixedly connected on the left side of backing plate, The left side of the rubber sleeve one is contacted with the inner wall of air inlet pipe.The nucleic acid extraction room of air inlet duct and conventional instrument for extracting nucleic acid connects It is logical.
Preferably, the surface of the connecting rod is fixedly connected with sliding block, and the inside of the positioning pipe offers sliding slot, described Sliding block is slidably connected at the inside of sliding slot.
Preferably, the left end of the connecting rod is fixedly connected with baffle, is fixedly connected on sealing shroud on the right side of the baffle Left side.
Preferably, rubber sleeve two, the left side of the rubber sleeve two and biography are fixedly connected on the right side of the turning set inner wall The right contact of dynamic set, the top and bottom of the drive sleeve are fixedly connected to the bull stick on the outside of positioning pipe.
Preferably, the right end of the connecting rod is provided with bindiny mechanism, the bindiny mechanism include position-arresting disk, limit sleeve and Gag lever post, the position-arresting disk are fixedly connected on the right end of connecting rod, and the limit sleeve is socketed in the surface of position-arresting disk, the limit Bar is fixedly connected on the surface of limit sleeve, and the gag lever post is fixedly connected on the inside of drive sleeve, institute far from one end of limit sleeve The shape of the shape and sleeve surface of stating drive sleeve surface is T shape.
Compared with prior art, the present invention provides a kind of instrument for extracting nucleic acid, have it is following the utility model has the advantages that
1, the instrument for extracting nucleic acid takes out the air of extraction apparatus body interior by turning set and positioning pipe by blower It is dynamic, so that extraneous air is entered the inside of extraction apparatus ontology by air inlet pipe and air inlet duct, then by air cleaning unit to extraction Gas carry out purified treatment, thus have the gas of volatilization can be carried out extraction purification the advantages of, solve existing Instrument for extracting nucleic acid does not have certain gas treatment means, and when opening instrument for extracting nucleic acid, the gas that reagent volatilizes will be let out Air pollution is caused out, and is retained in the portion gas in instrument for extracting nucleic acid and is then easy to cause the reagent for extracting nucleic acid next time The problem of pollution, improves the practicability of instrument for extracting nucleic acid.
2, the instrument for extracting nucleic acid can carry out a degree of pulling to backing plate by elastic force, make to pad by the way that tension spring is arranged Plate can be sealed by rubber sleeve a pair of air inlet pipe, improve the stability of backing plate, can by setting sliding block and sliding slot Connecting rod is limited, the phenomenon that connecting rod rotates when moving is avoided, improves stabilization when connecting rod movement Property, by the way that rubber sleeve two is arranged, the gap between turning set and drive sleeve can be sealed, avoid gas and be easy from seam The phenomenon that gap is blown out improves the leakproofness of drive sleeve, by the way that the shape of the shape on drive sleeve surface and sleeve surface to be all provided with It is set to T shape, sleeve can be limited, sleeve is avoided and occurs the phenomenon that being detached from fixing sleeve when in use, improve sleeve Stability when in use.
Detailed description of the invention
Fig. 1 is schematic structural view of the invention;
Fig. 2 is the enlarged diagram in Fig. 1 at A;
Fig. 3 is the enlarged diagram in Fig. 1 at B.
In figure: 1 extraction apparatus ontology, 2 air inlet pipe, 3 fixing sleeves, 4 positioning pipes, 5 air cleaning units, 6 blowers, 7 connecting rods, 8 sealing shrouds, 9 drive sleeves, 10 turning sets, 11 sealing mechanisms, 111 sleeves, 112 backing plates, 113 air inlet ducts, 114 rubber sleeves one, 115 Tension spring, 12 sliding blocks, 13 sliding slots, 14 baffles, 15 rubber sleeves two, 16 bull sticks, 17 bindiny mechanisms, 171 position-arresting disks, 172 limit sleeves, 173 gag lever posts.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.
Please refer to Fig. 1-3, a kind of instrument for extracting nucleic acid, including extraction apparatus ontology 1, the left side of extraction apparatus ontology 1 be communicated with into The inside of tracheae 2, air inlet pipe 2 is fixedly connected with fixing sleeve 3 by connecting plate, and the right side of extraction apparatus ontology 1 is communicated with positioning pipe 4, positioning pipe 4 is connected to the nucleic acid extraction room of instrument for extracting nucleic acid 1, and it is net that the top on 1 right side of extraction apparatus ontology is fixedly installed with air Makeup sets 5, and air inlet pipe 2 is internally provided with sealing mechanism 11, and sealing mechanism 11 includes sleeve 111, backing plate 112, air inlet duct 113, rubber sleeve 1 and tension spring 115, sleeve 111 are slidably connected at the inside of fixing sleeve 3, and backing plate 112 is fixedly connected on sleeve 111 right side, air inlet duct 113 are provided with the right side of 2 inner wall top and bottom of air inlet pipe, air inlet duct 113 and instrument for extracting nucleic acid 1 The connection of nucleic acid extraction room, tension spring 115 are fixedly connected on the right side of 111 inner wall of sleeve, and the left end of tension spring 115 is fixedly connected on fixation The left side of 3 inner walls is covered, rubber sleeve 1 is fixedly connected on the left side of backing plate 112, left side and the air inlet pipe 2 of rubber sleeve 1 Inner wall contact can carry out a degree of pulling to backing plate 112 by elastic force, enable backing plate 112 by the way that tension spring 115 is arranged It is enough sealed by one 114 pairs of air inlet pipe 2 of rubber sleeve, improves the stability of backing plate 112, the right side of extraction apparatus ontology 1 is solid Surely it is connected with the blower 6 positioned at 4 top of positioning pipe, the output end of blower 6 is connected to by connecting tube in 5 bottom of air cleaning unit Left side, the inner transverse of positioning pipe 4 is provided with connecting rod 7, and the surface of connecting rod 7 is fixedly connected with sliding block 12, positioning pipe 4 Inside offers sliding slot 13, and sliding block 12 is slidably connected at the inside of sliding slot 13, can be to even by setting sliding block 12 and sliding slot 13 Extension bar 7 is limited, and the phenomenon that connecting rod 7 rotates when moving is avoided, and improves the stabilization when movement of connecting rod 7 Property is fixedly connected with sealing shroud 8 on the left of 7 surface of connecting rod, and the left end of connecting rod 7 is fixedly connected with baffle 14, baffle 14 Right side is fixedly connected on the left side of sealing shroud 8, and the surface of sealing shroud 8 is contacted with the inner wall of positioning pipe 4, the right side of 4 inner cavity of positioning pipe Side is threaded with drive sleeve 9, and turning set 10 is socketed on the right side of 9 surface of drive sleeve, and the right side of 10 inner wall of turning set is fixed to be connected It is connected to rubber sleeve 2 15, the left side of rubber sleeve 2 15 and the right contact of drive sleeve 9, the top and bottom of drive sleeve 9 is fixed It is connected with the bull stick 16 for being located at 4 outside of positioning pipe, it, can be between turning set 10 and drive sleeve 9 by setting rubber sleeve 2 15 Gap be sealed, avoid gas and be easy the phenomenon that blowing out from gap, improve the leakproofness of drive sleeve 9, connecting rod 7 Right end is provided with bindiny mechanism 17, and bindiny mechanism 17 includes position-arresting disk 171, limit sleeve 172 and gag lever post 173, and position-arresting disk 171 is solid Surely it is connected to the right end of connecting rod 7, limit sleeve 172 is socketed in the surface of position-arresting disk 171, and gag lever post 173 is fixedly connected on limit The surface of set 172, gag lever post 173 are fixedly connected on the inside of drive sleeve 9 far from one end of limit sleeve 172,9 surface of drive sleeve Shape and the shape on 111 surface of sleeve are T shape, by the way that the shape on 9 surface of drive sleeve and the shape on 111 surface of sleeve to be all provided with It is set to T shape, sleeve 111 can be limited, sleeve 111 is avoided and occurs the phenomenon that being detached from fixing sleeve 3 when in use, improve The input terminal of the stability of sleeve 111 when in use, blower 6 is connected to by bellows on the right side of turning set 10.
When in use, user holds bull stick 16 first and rotates, and bull stick 16 drives drive sleeve 9 to rotate, and drive sleeve 9 is then Connecting rod 7 can be driven to be moved to the left by gag lever post 173 and limit sleeve 172, then start blower 6, blower 6 can then pass through rotation Set 10 and positioning pipe 4 twitch the air inside extraction apparatus ontology 1, since extraction apparatus ontology 1 is sealing state, to take out Backing plate 112 can be pulled to the right while dynamic air, stretch backing plate 112 to tension spring 115 with moving sleeve 111, this When extraneous air can then enter the inside of extraction apparatus ontology 1 by air inlet pipe 2 and air inlet duct 113, and inside extraction apparatus ontology 1 Gas then can by blower 6 inject air cleaning unit 5 inside carry out purified treatment, can be to the gas of volatilization to reach Body carries out the effect of extraction purification.
In conclusion the instrument for extracting nucleic acid, by blower 6 by turning set 10 and positioning pipe 4 to extraction apparatus ontology 1 inside Air is twitched, and so that extraneous air is entered the inside of extraction apparatus ontology 1 by air inlet pipe 2 and air inlet duct 113, then by air The gas of 5 pairs of purification device extractions carries out purified treatment, so that the excellent of extraction purification can be carried out to the gas of volatilization by having Point solves existing instrument for extracting nucleic acid and does not have certain gas treatment means, when opening instrument for extracting nucleic acid, reagent volatilization Gas out, which will release, causes air pollution, and is retained in the portion gas in instrument for extracting nucleic acid and is then easy to extracting next time The problem of reagent of nucleic acid pollutes improves the practicability of instrument for extracting nucleic acid.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment Intrinsic element.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (6)

1. a kind of instrument for extracting nucleic acid, including extraction apparatus ontology (1), it is characterised in that: the left side of the extraction apparatus ontology (1) is connected to Have air inlet pipe (2), the inside of the air inlet pipe (2) is fixedly connected with fixing sleeve (3) by connecting plate, the extraction apparatus ontology (1) it is communicated on the right side of positioning pipe (4), the top on the right side of the extraction apparatus ontology (1) is fixedly installed with air cleaning unit (5), the blower (6) being fixedly connected on the right side of the extraction apparatus ontology (1) at the top of positioning pipe (4), the blower (6) Output end is connected to by connecting tube in the left side of air cleaning unit (5) bottom, and the inner transverse of the positioning pipe (4) is provided with Connecting rod (7) is fixedly connected with sealing shroud (8) on the left of connecting rod (7) surface, the surface of the sealing shroud (8) and fixed The inner wall of position pipe (4) contacts, and is threaded with drive sleeve (9) on the right side of positioning pipe (4) inner cavity, drive sleeve (9) table It is socketed on the right side of face turning set (10), the input terminal of the blower (6) is connected to by bellows on the right side of turning set (10).
2. a kind of instrument for extracting nucleic acid according to claim 1, it is characterised in that: air inlet pipe (2) are internally provided with Sealing mechanism (11), the sealing mechanism (11) include sleeve (111), backing plate (112), air inlet duct (113), rubber sleeve one (114) and tension spring (115), the sleeve (111) are slidably connected at the inside of fixing sleeve (3), and the backing plate (112) is fixedly connected On the right side of sleeve (111), the air inlet duct (113) is provided with the right side of air inlet pipe (2) inner wall top and bottom, the tension spring (115) it is fixedly connected on the right side of sleeve (111) inner wall, the left end of the tension spring (115) is fixedly connected on fixing sleeve (3) inner wall Left side, the rubber sleeve one (114) is fixedly connected on the left side of backing plate (112), the left side of the rubber sleeve one (114) with into The inner wall of tracheae (2) contacts.
3. a kind of instrument for extracting nucleic acid according to claim 1, it is characterised in that: the surface of the connecting rod (7) is fixed to be connected It is connected to sliding block (12), the inside of the positioning pipe (4) offers sliding slot (13), and the sliding block (12) is slidably connected at sliding slot (13) Inside.
4. a kind of instrument for extracting nucleic acid according to claim 1, it is characterised in that: the left end of the connecting rod (7) is fixed to be connected It is connected to baffle (14), the left side of sealing shroud (8) is fixedly connected on the right side of the baffle (14).
5. a kind of instrument for extracting nucleic acid according to claim 1, it is characterised in that: the right side of turning set (10) inner wall is solid Surely rubber sleeve two (15) are connected, the left side of the rubber sleeve two (15) and the right contact of drive sleeve (9), the drive sleeve (9) top and bottom is fixedly connected to the bull stick (16) on the outside of positioning pipe (4).
6. a kind of instrument for extracting nucleic acid according to claim 2, it is characterised in that: the right end of the connecting rod (7) is provided with Bindiny mechanism (17), the bindiny mechanism (17) include position-arresting disk (171), limit sleeve (172) and gag lever post (173), the limit Position disk (171) is fixedly connected on the right end of connecting rod (7), and the limit sleeve (172) is socketed in the surface of position-arresting disk (171), institute State the surface that gag lever post (173) is fixedly connected on limit sleeve (172), the one end of the gag lever post (173) far from limit sleeve (172) It is fixedly connected on the inside of drive sleeve (9), the shape on drive sleeve (9) surface and the shape on sleeve (111) surface are T Shape.
CN201910696199.9A 2019-07-30 2019-07-30 Nucleic acid extraction instrument Active CN110257372B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910696199.9A CN110257372B (en) 2019-07-30 2019-07-30 Nucleic acid extraction instrument

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Application Number Priority Date Filing Date Title
CN201910696199.9A CN110257372B (en) 2019-07-30 2019-07-30 Nucleic acid extraction instrument

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CN110257372A true CN110257372A (en) 2019-09-20
CN110257372B CN110257372B (en) 2024-01-09

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113416630A (en) * 2021-06-21 2021-09-21 上海金鹏分析仪器有限公司 Nucleic acid purification system with anti-pollution effect

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN206751853U (en) * 2017-05-01 2017-12-15 任碧琼 A kind of full-automatic nucleic acid extraction, amplification integrated apparatus
CN206902130U (en) * 2017-07-03 2018-01-19 广州瑞能医学科技有限公司 A kind of anti-pollution instrument for extracting nucleic acid
CN209046942U (en) * 2018-08-29 2019-06-28 深圳市杰恩凯电子科技有限公司 A kind of Gas controller with dust reduction capability

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN206751853U (en) * 2017-05-01 2017-12-15 任碧琼 A kind of full-automatic nucleic acid extraction, amplification integrated apparatus
CN206902130U (en) * 2017-07-03 2018-01-19 广州瑞能医学科技有限公司 A kind of anti-pollution instrument for extracting nucleic acid
CN209046942U (en) * 2018-08-29 2019-06-28 深圳市杰恩凯电子科技有限公司 A kind of Gas controller with dust reduction capability

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113416630A (en) * 2021-06-21 2021-09-21 上海金鹏分析仪器有限公司 Nucleic acid purification system with anti-pollution effect

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