Portable detection of nucleic acids case
Technical field
Technical field of pharmaceutical biotechnology is the utility model is related to, specifically portable detection of nucleic acids case.
Background technology
It is well known that nucleic acid and pathogenic genes and genetic disease, gene expression regulation, cell Proliferation and apoptosis and cancer
Occurrence and development there is important contact.The tissue of biology, bacterium, virus are respectively provided with the nucleotide sequence of uniqueness, these are specific
The detection of sequence plays an important role in fields such as genetic analysis, medical diagnosis on disease, food pollution, forensic identification and environmental monitorings.
With the development of science and technology, detection of nucleic acids has been increasingly being applied to medical diagnosis, animal epidemic diagnoses,
The fields such as food safety detection, and there is high sensitivity, without user's blood sampling by nucleic acid to detect pathogenic bacteria.Mesh
Before, common nucleic acid amplification method has based on classical Common Polymerase Chain Reaction(PCR)Amplification technique and emerging constant temperature
Amplification technique, such as cross primer isothermal amplification technology(CPA), loop-mediated isothermal amplification technology(LAMP), strand displacement amplification
(SDA), rely on unwindase amplification(HDA), restructuring polymeric enzymatic amplification(RPA), recombinase-mediated amplification(RAA)Deng.
Site Detection requirement is quick, accurate, easy to operate, and sample virus nucleic acid detection technique universal at present includes sample
This nucleic acid extraction, sample rna reverse transcription and sample DNA positive detection etc., these are required to the talent of specialty, technical equipment and clean
Net environment, and sample is also required to the substantial amounts of time during provincial, and municipal level test in laboratory is sent to, and these conditions are all sample
This Positive test causes greatly difficulty, also adds difficulty for the control of epidemic situation.
Detection of nucleic acids unit type currently on the market is very much, the temperature control mode of its nucleic acid amplification have air heating cooling,
The modes such as semiconductor chilling plate temperature control, heating film temperature control, infrared temperature control, but reagent and sample are generally to be reacted inside PCR pipe,
There are power supply energy loss is big, reagent and sample consumption are big, have with high costs, volume it is big it is heavy, complicated, to using
The shortcomings of person's competency profiling is high.
Utility model content
The utility model provides portable detection of nucleic acids case, and the detection case is easy to carry, full-featured, as a result accurate high
Effect, safety easy to operate, adapts to a variety of environment, and mutual collision belt between equipment can be avoided in case in carrying and transport process
Unnecessary loss.
The utility model be solve the technical solution that uses of above-mentioned technical problem for:Portable detection of nucleic acids case, including case
Body and case lid, box house are vertically arranged with multiple risers, and the inside of babinet is divided into several and is used to put by multiple risers
The locating slot of equipment, is detachably connected, two of which riser between multiple risers and between riser and babinet by buckle
Top offer four telescopic rod grooves, telescopic rod is vertically installed with telescopic rod groove, sterile work is fixedly installed on telescopic rod
Make platform, cover wall offers the aseptic working platform putting groove with aseptic working platform location matches;
The bottom of box is horizontally disposed with two supporting rods along babinet length direction, and the both ends of supporting rod are each provided with a connection
Part, supporting rod are removably connected with pull rod and the handle being connected with pull rod by connector;Bottom of box four corners are equipped with ten thousand
To wheel, wherein, on the length direction of babinet, micromachine is provided with two universal wheels of babinet the same end, it is miniature
Motor driving universal wheel rotates synchronously.
As a preferred embodiment, the locating slot of the box house is arranged to eight.
As a preferred embodiment, eight locating slots are respectively power slot, PCR instrument groove, mini centrifugation trough, pipette tips box
Groove, power adaptation tank, 1-10ul liquid-transfering guns putting groove, 20-200ul liquid-transfering guns putting groove and 100-1000ul liquid-transfering guns are put
Groove.
As a preferred embodiment, it is provided with solar panel on the outer wall of the case lid.
As a preferred embodiment, the vent valve for balance detection case inner and outer air pressure is additionally provided with the babinet.
As a preferred embodiment, the babinet side is hinged by shaft and case lid, opposite with shaft another on babinet
Side is equipped with some locks for being used to fasten babinet and case lid.
As a preferred embodiment, the telescopic rod includes interior bar and outer bar, and the outer bar is set in interior bar, interior bar side
Wall is equipped with multiple snap-ons, corresponds on outer bar side wall at the position of snap-on and is equipped with multiple limit holes.
As a preferred embodiment, the height of the riser is not more than the height of babinet.
As a preferred embodiment, the aseptic working platform is rectangular box, and the top of aseptic working platform is equipped with ultraviolet
Lamp, one end of aseptic working platform are provided with the ultraviolet violet light switch being electrically connected with ultraviolet lamp, one of side of aseptic working platform
Offer door.
As a preferred embodiment, the connector includes knob and limited block, and the radius of the pull rod is less than supporting rod
Radius, and the circular hole for wearing knob is both provided with pull rod and supporting rod, limited block is arranged on the inner wall of supporting rod.
Compared with prior art, the utility model has the advantages that:
(1)The utility model provides portable detection of nucleic acids case, without other auxiliary equipment, can realize field condition
Viral nucleic acid detects, and realizes the function of detecting whenever and wherever possible;This detection case is full-featured, easy to operate, can be when 1.5 is small
Within complete sample rna extraction, the work of detection of nucleic acids and data analysis, by the pattern detection time progress of original 1-3 days
Pole is significantly shortened, and can be determined within the shortest time and symptom management;Bluetooth of mobile phone or tablet computer with it is portable
PCR instrument connection uses, can be according to the flexible option program of situation on the spot, real-time monitoring data;
(2)The utility model provides portable detection of nucleic acids case, this detection case is widely used, and it is anti-to be particularly suitable for health
Epidemic disease department, superintendent office scene carry out the quick detection of livestock and poultry sample and are used, to promptly and accurately judging whether livestock and poultry sample infects disease
Poison plays the role of quick examination;Telescopic rod is provided among babinet, aseptic working platform, sterile work are fixedly installed on telescopic rod
The top for making platform is equipped with ultraviolet lamp, and ultraviolet lamp is opened and can sterilized, avoid during experimental implementation between various viruses with
And when detecting a kind of virus, the pollution caused by sampling process;Meanwhile telescopic rod stretch, can using aseptic working platform as
Operation console, easy to the operation in experimentation;Aseptic working platform side offers door, easy to laboratory technician in aseptic working platform into
Sampling in row RNA extractions and detection of nucleic acids;Offer in case lid and put with the aseptic working platform of aseptic working platform location matches
Groove is put, when closing detection case, telescopic rod is shunk, and aseptic working platform is placed in aseptic working platform putting groove, is saved space, is made
With conveniently;
(3)The utility model provides portable detection of nucleic acids case, on the length direction of babinet, positioned at babinet one end
Be provided with micromachine on two universal wheels, micromachine driving universal wheel rotates synchronously, meanwhile, supporting rod by connector with
Handle is detachably connected, in this way, the universal wheel equipped with micromachine of one end can be used for using when going up a slope, the other end does not fill electricity
The universal wheel of machine can be used for descending or walk level road, and knob is detachably arranged at the both sides of babinet by connector, meanwhile, support
Four knobs set on bar, when babinet is kept flat, knob contacts to earth case supports, can prevent universal wheel from rolling, and rises
To fixation.
Brief description of the drawings
Fig. 1 is the utility model overall structure diagram;
Fig. 2 is supporting rod and handle Detachable connection structure schematic diagram;
Reference numeral:1st, babinet, 2, case lid, 3, pull rod, 4, handle, 5, universal wheel, 6, lock, 7, locating slot, 8, riser,
9th, vent valve, 10, micromachine, 11, aseptic working platform, 12, telescopic rod, 13, shaft, 14, aseptic working platform putting groove, 15,
Supporting rod, 16, knob, 17, ultraviolet lamp, 18, door, 19, ultraviolet violet light switch, 20, limited block.
Embodiment
Elaborate below in conjunction with the accompanying drawings to the embodiment of the utility model, the present embodiment is with the utility model technical side
Premised on case, detailed embodiment and specific operating process are given, but the scope of protection of the utility model is not limited to down
The embodiment stated.
Portable detection of nucleic acids case, including babinet 1 and case lid 2,1 inner vertical of babinet are provided with multiple risers 8, Duo Geli
The inside of babinet 1 is divided into several locating slots 7 for being used to put equipment by plate 8, as preference, the multiple riser 8 wraps
Four risers and three risers parallel to 1 length direction of babinet parallel to 1 width of babinet are included, four parallel to babinet
In the riser of 1 width, wherein being of same size between the length of three risers and cabinet wall, the length of another riser
Degree is less than the width between cabinet wall;In three risers parallel to 1 length direction of babinet, one of riser and length are small
The riser square crossing of width is set between cabinet wall, in the length and babinet that two other riser is vertically disposed on
Between any two for three risers being of same size between wall, to form eight locating slots 7;It is between multiple risers 8 and vertical
It is detachably connected between plate 8 and babinet 1 by buckle, the top of two of which riser 8 offers four telescopic rod grooves, stretches
Telescopic rod 12 is vertically installed with bar groove, aseptic working platform 11 is fixedly installed on telescopic rod 12,2 inner wall of case lid opens up being and not being
The aseptic working platform putting groove 14 of 11 location matches of bacterium workbench;
1 bottom of babinet is horizontally disposed with two supporting rods 15 along 1 length direction of babinet, and the both ends of supporting rod 15 are each provided with one
A connector, supporting rod 15 is removably connected with pull rod 3 and the handle 4 being connected with pull rod 3 by connector, as preferred
, the babinet 1, case lid 2, pull rod 3 and handle 4 are made of PP+ glass synthetic materials, strong, acidproof with anti-impact force
The advantages that caustic corrosion, fire-retardant high, non-aging and waterproof;Universal wheel 5 is equipped with 1 bottom four corners of babinet, wherein, in babinet 1
On length direction, micromachine 10 is provided with two universal wheels 5 of 1 the same end of babinet, micromachine 10 drives universal
Wheel 5 rotates synchronously.
Above be the utility model basic embodiment, can more than on the basis of be improved further, optimize or limit
It is fixed.
Further, the locating slot 7 inside the babinet 1 is arranged to eight.
Further, eight locating slots 7 are respectively power slot, PCR instrument groove, centrifugation trough, pipette tips box groove, power supply adaptor
Groove, 1-10ul liquid-transfering guns putting groove, 20-200ul liquid-transfering guns putting groove and 100-1000ul liquid-transfering gun putting grooves, as preferred
, the PCR instrument is portable PCR instrument, and the centrifuge is palm centrifuge or mini centrifuge, and power supply can be centrifuge
Stable 220V alternating currents are provided with portable PCR instrument, the centrifuge can play centrifugation in sample nucleic acid extraction process and make
With portable PCR instrument plays whether detection sample nucleic acid contains specific virus sequence so as to identify the work whether sample infects
With the pipette tips box is essential consumptive material in extraction and detection process, and the power supply adaptor is PCR instrument and power source charges
The voltage and current of stable matching is provided, using before detection case, need to ensure power supply remaining capacity more than 50% can completely into
Row one-time detection.In addition, instrument and accessory in the casees such as USB flash disk, corresponding power line and connecting line, every kind of USB flash disk pair are further included in babinet 1
A kind of viral diagnosis is answered, PCR instrument can be with the operation sequence in automatic identification USB flash disk, and is out of order in mobile phone or tablet computer
When can replace its use, it is efficient and convenient.
Further, solar panel is provided with the outer wall of the case lid 2, as preference, solar panel is set
In the groove that 1 outer wall of babinet is set, in the case of sunny, outdoor it can charge at any time.
Further, the vent valve 9 for balance detection case inner and outer air pressure is additionally provided with the babinet 1, as preferred
, the vent valve 9 is PP+ glasses+metal brass screws material, and for pressure in release case, waterproof is used.
Further, 1 side of babinet is hinged by shaft 13 and case lid 2, opposite with shaft 13 another on babinet 1
Side is equipped with some locks 6 for babinet 1 and case lid 2 to be fastened, and 1 design structure of babinet is reasonable, and babinet 1 and case lid 2 can be in 120
Degree is opened, and can be worked normally in a variety of contexts.
Further, the telescopic rod 12 includes interior bar and outer bar, and the outer bar is set in interior bar, is set on interior bar side wall
There are multiple snap-ons, correspond on outer bar side wall at the position of snap-on and be equipped with multiple limit holes.
Further, the height of the riser 8 is not more than the height of babinet 1.
Further, the aseptic working platform 11 is rectangular box, and the top of aseptic working platform 11 is equipped with ultraviolet lamp 17,
One end of aseptic working platform 11 is provided with the ultraviolet violet light switch 19 being electrically connected with ultraviolet lamp 17, aseptic working platform 11 one of them
Side offers door 18, and the door 18 has two, easy to laboratory technician by hand put in the inner progress RNA extractions of aseptic working platform 11 and
Sampling in detection of nucleic acids.
Further, as shown in Fig. 2, the connector includes knob 16 and limited block 20, the radius of pull rod 3 is less than support
The radius of bar 15, and the circular hole for wearing knob 16 is both provided with pull rod 3 and supporting rod 15, limited block 20 is arranged on support
On the inner wall of bar 15.Wherein, the both ends of each supporting rod 15 are mounted on a knob 16.
Wherein, Fig. 2 is the supporting rod and the rip cutting profile of handle under the main view direction of Fig. 1, as preference, in Fig. 1
Working status, height when knob 16 is placed vertically is more than the diameter of universal wheel 5, to reach when babinet 1 is kept flat, two supports
Four knobs 16 at 15 both ends of bar contact to earth, and babinet 1 is supported, can prevent universal wheel 5 from rolling, play fixation;Branch
Strut 15 and pull rod 3 are round bar, and pull rod 3 is telescopic rod, can be with telescopic adjustment after pull rod 3 is fixed by connector
The length of pull rod 3.
The step of carrying out the detection of livestock and poultry sample nucleic acid using the portable detection of nucleic acids case of the utility model is exemplified below
(all equipment, detecting instrument etc. are article in the portable detection of nucleic acids case case of the utility model, and detection reagent is separately to purchase this inspection
Measuring tank matched reagent):
Embodiment 1:Avian flu virus detection.
RNA is extracted:The 200 μ l of buccal swab of sample to be tested are taken, coordinate 1000 μ l pipette tips to add extraction examination using liquid-transfering gun
600 μ l of buffer A in agent box stand 3 minutes after mixing, using palm centrifuge 1 minute, are matched somebody with somebody using liquid-transfering gun
Closing pipette tips takes 400 μ l supernatants to add in the new EP pipes without RNase supporting in extracts kit, coordinates liquid-transfering gun and pipette tips
Buffer B in 200 μ l extracts kits is added, is added after mixing in adsorption column supporting in extracts kit, is centrifuged one minute;
The waste liquid in collecting pipe is discarded, adding 800 μ l buffer solutions C into adsorption column using liquid-transfering gun centrifuges one minute;Collecting pipe is put
Enter in palm centrifuge and centrifuge 1min after trim, abandon collecting pipe and be put into adsorption column in the 1.5mlEP pipes of new no RNase,
60ul eluents are added, palm centrifuge is put into, 1min is centrifuged after trim, the liquid being collected into EP pipes is sample rna, this
Outside, the safeguard procedures of specialty must be carried out during sample collection, any position of skin must not direct contact measured sample;Liquid relief
Rifle needs to recall to maximum range after;Palm centrifuge and portable PCR instrument are both needed to symmetrically placed sample in use.
Detection of nucleic acids:Added with liquid-transfering gun extraction 20ul amplification buffers in amplification system, fully add extraction after dissolving
Sample rna 5ul, same method is separately added into 5ul positive controls, negative control in detection kit.After mixing
Use palm centrifuge brief centrifugation.The 100ulEP pipes for needing to detect symmetrically are put into the sample aperture of portable PCR instrument, and
Remember numbering corresponding thereto, each plug in after portable PCR instrument is connected with tablet computer.Open on tablet computer
The corresponding software of portable PCR instrument, the detection program that selection matches with virus to be detected, determines the same computer of portable PCR instrument
It is successfully connected, click brings into operation.
Data analysis:Preserved after EP (end of program) and reopen preservation as a result, using software to data result carry out
Analysis, by data and curves and negative positive control judge its sample to be tested whether virus infection.
Embodiment 2:Detect Pseudorabies virus
DNA is extracted:The serum solution 10ul of the lesions position of test individual is taken to be diluted with water to 200 μ l, in extracts kit
600 μ l of buffer A stand 3 minutes after mixing, using palm centrifuge 5000r/min centrifuge 1 minute, use liquid relief
Rifle coordinates pipette tips to take 400 μ l supernatants to add in extracts kit in the supporting new EP pipes without RNase, coordinate liquid-transfering gun and
Pipette tips add buffer B in 200 μ l extracts kits, are added after mixing in adsorption column supporting in extracts kit, centrifugation one
Minute;The waste liquid in collecting pipe is discarded, adding 800 μ l buffer solutions C into adsorption column using liquid-transfering gun centrifuges one minute;It will collect
Pipe is put into palm centrifuge 5000r/min after trim and centrifuges 1min, abandons collecting pipe and adsorption column is put into new no RNase
In 1.5mlEP pipes, 60ul eluents are added, are put into palm centrifuge, 5000r/min centrifuges 1min after trim, is collected in EP pipes
To liquid be sample DNA.
Detection of nucleic acids:Added with liquid-transfering gun extraction 20ul amplification buffers in amplification system, fully add extraction after dissolving
Sample rna 5ul, same method is separately added into 5ul positive controls, negative control in detection kit.After mixing
Use palm centrifuge brief centrifugation.The 100ulEP pipes for needing to detect symmetrically are put into the sample aperture of portable PCR instrument, and
Remember numbering corresponding thereto.Each plug in after portable PCR instrument is connected with tablet computer.Open on tablet computer
The corresponding software of portable PCR instrument, the detection program that selection matches with virus to be detected, determines the same computer of portable PCR instrument
It is successfully connected, click brings into operation.
Data analysis:Preserved after EP (end of program) and reopen preservation as a result, using software to data result carry out
Analysis, by data and curves and negative positive control judge its sample to be tested whether virus infection.
Embodiment 3:USB connecting detection Pseudorabies virus
DNA is extracted:Take the serum solution 10ul of the lesions position of test individual to be diluted with water as 200 μ l, matched somebody with somebody using liquid-transfering gun
Close the 600 μ l of buffer A that 1000 μ l pipette tips are added in extracts kit and stand 3 minutes after mixing, use palm centrifuge
5000r/min is centrifuged 1 minute, coordinates pipette tips to take 400 μ l supernatants to add using liquid-transfering gun supporting new in extracts kit
In EP pipes without RNase, coordinate liquid-transfering gun and pipette tips to add buffer B in 200 μ l extracts kits, extraction examination is added after mixing
In agent box in supporting adsorption column, centrifuge one minute;The waste liquid in collecting pipe is discarded, is added using liquid-transfering gun into adsorption column
800 μ l buffer solutions C are centrifuged one minute;Collecting pipe is put into palm centrifuge 5000r/min after trim and centrifuges 1min, abandons receipts
Adsorption column is put into the 1.5mlEP pipes of new no RNase by collector, is added 60ul eluents, palm centrifuge is put into, after trim
5000r/min centrifuges 1min, and the liquid being collected into EP pipes is sample DNA.
Detection of nucleic acids:Added with liquid-transfering gun extraction 20ul amplification buffers in amplification system, fully add extraction after dissolving
Sample rna 5ul, same method is separately added into 5ul positive controls, negative control in detection kit.After mixing
Use palm centrifuge brief centrifugation.The 100ulEP pipes for needing to detect symmetrically are put into the sample aperture of portable PCR instrument, and
Remember numbering corresponding thereto.Detection program corresponding with the virus is stored in USB flash disk with .PPF forms, by PCR with even
Connect and the USB flash disk is inserted into portable PCR instrument bottom by USB jack after power supply, PCR instrument is by automatic identification procedure and brings into operation.
The ring of light of PCR instrument position base can be changed into the alternate colour of polychrome after end of run.
Data analysis:USB flash disk is pulled out on computer of the insertion equipped with PCR instrument software kit after EP (end of program), is selected in USB flash disk
Select preservation data opened with software after, judge whether its sample to be tested infects disease by data and curves and negative positive control
Poison.
Embodiment 4:USB connecting detection foot and mouth disease viruses
RNA is extracted:Take the blister fluid 10ul of the lesions position of test individual to be diluted with water as 200 μ l, matched somebody with somebody using liquid-transfering gun
Close the 600 μ l of buffer A that 1000 μ l pipette tips are added in extracts kit and stand 3 minutes after mixing, use palm centrifuge
5000r/min is centrifuged 1 minute, coordinates pipette tips to take 400 μ l supernatants to add using liquid-transfering gun supporting new in extracts kit
In EP pipes without RNase, coordinate liquid-transfering gun and pipette tips to add buffer B in 200 μ l extracts kits, extraction examination is added after mixing
In agent box in supporting adsorption column, centrifuge one minute;The waste liquid in collecting pipe is discarded, is added using liquid-transfering gun into adsorption column
800 μ l buffer solutions C are centrifuged one minute;Collecting pipe is put into palm centrifuge 5000r/min after trim and centrifuges 1min, abandons receipts
Adsorption column is put into the 1.5mlEP pipes of new no RNase by collector, is added 60ul eluents, palm centrifuge is put into, after trim
5000r/min centrifuges 1min, and the liquid being collected into EP pipes is sample rna.
Detection of nucleic acids:Added with liquid-transfering gun extraction 20ul amplification buffers in amplification system, fully add extraction after dissolving
Sample rna 5ul, same method is separately added into 5ul positive controls, negative control in detection kit.After mixing
Use palm centrifuge brief centrifugation.The 100ulEP pipes for needing to detect symmetrically are put into the sample aperture of portable PCR instrument, and
Remember numbering corresponding thereto.Detection program corresponding with the virus is stored in USB flash disk with .PPF forms, by PCR with even
Connect and the USB flash disk is inserted into portable PCR instrument bottom by USB jack after power supply, PCR instrument is by automatic identification procedure and brings into operation.
The ring of light of PCR instrument position base can be changed into the alternate colour of polychrome after end of run.
Data analysis:USB flash disk is pulled out on computer of the insertion equipped with PCR instrument software kit after EP (end of program), is selected in USB flash disk
Select preservation data opened with software after, judge whether its sample to be tested infects disease by data and curves and negative positive control
Poison.
This product the embodiment described is illustrative and not restrictive, and above-described embodiment is only to aid in understanding this reality
With new, therefore the utility model is not limited to the embodiment described in embodiment, every by those skilled in the art
Other embodiments that technical solution according to the present utility model is drawn, also belong to the scope of the utility model protection.