CN104818213B - Sliding type closed pathogen detection card box - Google Patents
Sliding type closed pathogen detection card box Download PDFInfo
- Publication number
- CN104818213B CN104818213B CN201510246613.8A CN201510246613A CN104818213B CN 104818213 B CN104818213 B CN 104818213B CN 201510246613 A CN201510246613 A CN 201510246613A CN 104818213 B CN104818213 B CN 104818213B
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- Prior art keywords
- layer ring
- internal layer
- annular
- pathogen detection
- ring
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Abstract
The invention provides a sliding type closed pathogen detection card box comprising a transparent box body, wherein the box body consists of a front wall, a rear wall and an annular enclosure; both the front wall and the rear wall are respectively provided with a circle of grooves, and two ring openings of the annular enclosure are embedded into the grooves of the front wall and the rear wall in a matching mode; the annular enclosure consists of an inner-layer annular ring and an outer-layer annular ring; the inner-layer annular ring is made of a transparent hard material, and a long elliptical hole is formed in the top surface of the inner-layer annular ring; the outer-layer annular ring is an annular transparent sheet, a round hole is formed in the sheet corresponding to the long elliptical hole, and the annular transparent sheet can slip along the inner-layer annular ring; and a base which is fixed at the bottom of the inner-layer annular ring is arranged in a cavity, a plurality of uniformly-arranged reagent tanks are formed in the base, and all reagents required for a detection process are pre-fed in the reagent tanks. According to the sliding type closed pathogen detection card box provided by the invention, a disease detection process can be completed in a basically closed environment, and the sliding type closed pathogen detection card box is disposable, so that the cross contamination problems and the infection hazards of experimenters can be greatly reduced.
Description
Technical field
The present invention relates to a kind of detection cartridge is and in particular to a kind of slidingtype closing pathogen detection cartridge is it is adaptable to just
Take in the biochemical detection system of formula, automatization, belong to biology, analytical chemistry and medical science.
Background technology
The detection process of disease, is required for pathogen nucleic acid is isolated and purified first, traditional separate nucleic acid technology
Step is numerous and diverse, time-consuming length, extraction efficiency are low, can contact toxic reagent, and is to carry out under an open environment, easily
Produce pollution.Disadvantages mentioned above just can be overcome well using the paramagnetic particle method nucleic acid extraction technology based on magnetic separation technique.The method
Do not need centrifugation, do not need to add multiple toxic reagents, safety non-toxic, fast and reliable, simple to operate.Need after the completion of nucleic acid extraction
Performing PCR amplification is entered to the sample nucleic acid extracting, finally to be detected.Whole process (nucleic acid extraction, PCR expands, detection)
Need to operate on different instruments to carry out, sample needs transfer repeatedly, this considerably increases the machine that sample produces cross-contamination
Rate, pollutes the operating process especially uncapping sampling detection after PCR amplification.Thus leading to last testing result not
Accurately so that extremely serious harm is all brought to patient.
Also some are had to enable sample preparation and test integrated apparatus in the market, such as Cepheid is public
The GeneXpert series of department.These overwhelming majority are all external instruments, and price is sufficiently expensive, are not appropriate for promoting, particularly
Hold explosive place in diseases such as Africa.For the especially strong disease of some infectiousness, such as Ebola virus, experiment is in envelope
Carry out in closed loop border, the probability of the infection virus of experimenter can be substantially reduced.Therefore, the present invention is existing according to current background
Shape, a kind of pathogen detection cartridge of the disease of design slidingtype closing, whole detection process can be allowed complete in closed environment
Become, detection can directly dispose cartridge after terminating.
Content of the invention
It is an object of the present invention to provide a kind of slidingtype closes pathogen detection cartridge, the structure of this cartridge is simple, whole disease
Sick detecting step is easy, quick, efficiency high, and with low cost, operating environment closes safety non-pollution.
The technical scheme is that：A kind of slidingtype closes pathogen detection cartridge, including a transparent cartridge, described
Box body is made up of the annular enclosure of antetheca, rear wall and；Described antetheca and rear wall are staggered relatively, all open at two opposing side edges
There is a circle groove, annular enclosure two circle mouth is supporting to be embedded in the groove of antetheca and rear wall, forms a cavity；
The enclosure of described annular is made up of internal layer ring and outer layer ring；Described internal layer ring is rigid transparent material, top surface
On offer an oblong hole；Described outer layer ring is an annular transparent thin slice, with oblong hole pair on thin slice
A circular port should be had in place, aperture is identical with the minor axis in oblong hole or more smaller than minor axis, described annular transparent thin slice
For a bendable material, it overlays on internal layer ring outer surface, can slide along internal layer ring；
In described cavity, the base of internal layer ring bottom is fixed in setting one, and base offers some evenly distributed examinations
Agent groove, is pre-loaded into all reagent of detection process needs in reagent trough；
Described detection cartridge is furnished with a pipettor, and described pipettor passes through the circular port of outer layer ring to above reagent trough,
Moved left and right in the range of oblong hole along internal layer ring by outer layer ring, realize sample-adding between different reagent troughs and
Liquid relief, completes whole disease detection process.
Further, the material of described reagent trough is inertia and non electrically conductive material, does not all occur with all agent formulations
Chemical reaction.
Further, described internal layer ring is PP material.Heat transfer efficiency height and optical clear.
Further, described antetheca and rear wall material are hard transparent plastic.Low cost is easy to make simple.
Further, the gap between described annular enclosure two circle mouth and groove seals the chamber so that whole with sealing ring
Body forms substantially airtight space.
The present invention also provides a kind of slidingtype to close the loading methods of pathogen detection cartridge, using the shifting with fluid suction head
Sample to be tested drawn by liquid device, enters in cartridge cavity from the circular port of outer layer ring, in the range of oblong hole, left and right is moved
Dynamic pipettor, sample is injected in corresponding reagent trough, and then piping and druming mixes, and cooperation external equipment is reacted.
The present invention also provides a kind of slidingtype to close the liquid relief method of pathogen detection cartridge, using the shifting with fluid suction head
Liquid device is drawn and is treated liquid relief, moves left and right correct reagent trough by inhaling the pipettor needing liquid relief in the range of oblong hole
Position, will treat that liquid relief is injected in reagent trough.
Advantages of the present invention：
The cartridge low cost of the present invention, structure is simple, easy to operate, and is easily integrated and realizes automatization.Disease detection
Process completes in the environment of base closed, and single use, greatly reduces cross-contamination issue and experimenter
Infection harm.Required external instrument device in detection greatly reduces.
Brief description
Fig. 1 cartridge overall appearance figure；
The internal upward view of Fig. 2；
Fig. 3 sectional structure chart.
Wherein：Base 11, reagent trough 12, outer layer ring 13, internal layer ring 14, sample-adding liquid relief hole (opens up on outer layer ring
Circular port) 15, elongated hole 16, groove 17, antetheca 18, rear wall 19.
Specific embodiment
Do and further explain with reference to embodiment and 1,2,3 couples of present invention of accompanying drawing.The following example is only used for
The bright present invention, but be not used to limit the practical range of the present invention.
A kind of slidingtype of the present invention closes pathogen detection cartridge, including a transparent cartridge, described box body by antetheca 18,
Rear wall 19 and an annular enclosure composition.Described antetheca 18 and rear wall 19 are hard transparent plastic, staggered relatively, two opposite faces
Edge all has a circle groove 17, and annular enclosure two circle mouth is supporting to be embedded in the groove of antetheca and rear wall, forms a sky
Chamber.Gap sealing ring sealing between described annular enclosure two circle mouth and groove so that whole cavity formed substantially airtight
Space.
The enclosure of described annular is made up of internal layer ring 14 and outer layer ring 13；Described internal layer ring 14 is clear hard material
Material, can be selected for that heat transfer efficiency is high and optically transparent PP material, and top surface offers an oblong hole.Described outer layer ring
Circle 13 is an annular transparent thin slice, and thin slice has a circular port 15, aperture and oblong with oblong hole corresponding position
Hole minor axis identical or more smaller than minor axis, described annular transparent thin slice be a bendable material, it overlays on outside internal layer ring
Surface, can slide along internal layer ring.
In described cavity, the base 11 of internal layer ring bottom is fixed in setting one, base offers some evenly distributed
Reagent trough 12, for nucleic acid extraction, amplification and detection.The material of described reagent trough is inertia and non electrically conductive material, and all
All there is not chemical reaction in agent formulations.It is pre-loaded into all reagent of detection process needs in reagent trough 12；As lysate, clean
Liquid, eluent, the required buffer of amplification, dNTP, enzyme, primer, probe and prevent reagent to be subject to mineral oil of thermal evaporation etc..
Described detection cartridge is furnished with a pipettor, and described pipettor passes through the circular port of outer layer ring to above reagent trough,
Moved left and right in the range of oblong hole along internal layer ring by outer layer ring, realize sample-adding between different reagent troughs and
Liquid relief, completes whole disease detection process.
Sample-adding process：Draw sample to be tested using the pipettor with fluid suction head, enter card from the circular port of outer layer ring
In box cavity, in the range of oblong hole, move left and right pipettor, sample is injected in corresponding reagent trough, then blows
Beat and mix, the heating outside cooperation, the equipment such as magnetically attractive is reacted.
Pipetting processes：Drawn using the pipettor with fluid suction head and treat liquid relief, ellipse in length by inhaling the pipettor needing liquid relief
Move left and right correct reagent groove location in the range of circular hole, will treat that liquid relief is injected in reagent trough.
Whole detection process only needs a liquid-transfering gun with fluid suction head, then coordinate outside corresponding function device it is possible to
The detection of pathogen is completed, experimental implementation is simple, safety in the environment of base closed.
The preferred embodiment of the present invention described in detail above, but, the present invention is not limited in above-mentioned embodiment
Detail, in the range of the technology design of the present invention, multiple equivalents can be carried out to technical scheme, this
A little equivalents belong to protection scope of the present invention.
Claims (5)
1. a kind of slidingtype closing pathogen detection cartridge it is characterised in that：Including a transparent cartridge, described box body is by front
Wall, rear wall and one annular enclosure composition；Described antetheca and rear wall are staggered relatively, all have a circle ditch at two opposing side edges
Groove, annular enclosure two circle mouth is supporting to be embedded in the groove of antetheca and rear wall, forms a cavity；
The enclosure of described annular is made up of internal layer ring and outer layer ring；Described internal layer ring is rigid transparent material, and top surface is opened
It is provided with an oblong hole；Described outer layer ring is an annular transparent thin slice, with oblong hole corresponding position on thin slice
Have a circular port, aperture is identical with the minor axis in oblong hole or more smaller than minor axis, described annular transparent thin slice is one
Bendable material, it overlays on internal layer ring outer surface, can slide along internal layer ring；
In described cavity, the base of internal layer ring bottom is fixed in setting one, and base offers some evenly distributed reagent
Groove, is pre-loaded into all reagent of detection process needs in reagent trough；
Described detection cartridge is furnished with a pipettor, and described pipettor passes through the circular port of outer layer ring to above reagent trough, passes through
Outer layer ring moves left and right in the range of oblong hole along internal layer ring, realizes the sample-adding between different reagent troughs and shifting
Liquid, completes whole disease detection process.
2. a kind of slidingtype closing pathogen detection cartridge according to claim 1 it is characterised in that：Described reagent trough
Material is inertia and non electrically conductive material, with all agent formulations, chemical reaction does not all occur.
3. a kind of slidingtype closing pathogen detection cartridge according to claim 1 it is characterised in that：Described internal layer ring
For PP material.
4. a kind of slidingtype closing pathogen detection cartridge according to claim 1 it is characterised in that：Described antetheca and after
Wall material is hard transparent plastic.
5. a kind of slidingtype closing pathogen detection cartridge according to claim 1 it is characterised in that：The enclosure of described annular
Gap sealing ring between two circle mouths and groove seals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510246613.8A CN104818213B (en) | 2015-05-14 | 2015-05-14 | Sliding type closed pathogen detection card box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510246613.8A CN104818213B (en) | 2015-05-14 | 2015-05-14 | Sliding type closed pathogen detection card box |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104818213A CN104818213A (en) | 2015-08-05 |
| CN104818213B true CN104818213B (en) | 2017-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510246613.8A Active CN104818213B (en) | 2015-05-14 | 2015-05-14 | Sliding type closed pathogen detection card box |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586256B (en) * | 2016-02-24 | 2017-11-03 | 东南大学 | A kind of closed pathogen detection cartridge |
| CN106526202B (en) * | 2016-11-17 | 2018-07-13 | 百奥森（江苏）食品安全科技有限公司 | A kind of closed pathogen detection cartridge |
| CN107964504B (en) * | 2017-10-28 | 2023-10-27 | 深圳职业技术学院 | Closed sample integrated detection device and detection method |
| CN110404594A (en) * | 2019-07-31 | 2019-11-05 | 深圳麦科田生物医疗技术有限公司 | Sample analysis kit |
| CN113150945B (en) * | 2021-04-14 | 2023-01-10 | 新羿制造科技（北京）有限公司 | Slide-type nucleic acid extraction and PCR amplification detection card box |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204702755U (en) * | 2015-05-14 | 2015-10-14 | 东南大学 | A kind of sliding type closes pathogen detection cartridge |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2426984Y (en) * | 2000-06-07 | 2001-04-18 | 中国水产科学研究院黄海水产研究所 | Prawn pathogeny detecting reagent box |
| US20070116613A1 (en) * | 2005-11-23 | 2007-05-24 | Donat Elsener | Sample tube and system for storing and providing nucleic acid samples |
| JP5707253B2 (en) * | 2011-06-27 | 2015-04-22 | 株式会社日立ハイテクノロジーズ | Cold reagent storage and nucleic acid analyzer |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204702755U (en) * | 2015-05-14 | 2015-10-14 | 东南大学 | A kind of sliding type closes pathogen detection cartridge |
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| CN104818213A (en) | 2015-08-05 |
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